Daftar Peralatan dalam penelitian Bioteknologi

1. T100 Thermal Cycler

Image

Description  The T100 thermal cycler is a small thermal cycler offering a

comprehensive set of convenient features in a small footprint. This
compact thermal cycler features an intuitive touch-screen user
interface to make running PCR easy. Thermal gradient technology
allows you to quickly optimize your reaction in a single run. With its
robust design, the T100 system is a reliable personal thermal cycler
that delivers exceptional performance for years — test drive the T100
thermal cycler today
 Intuitive touch screen — easy programming using a large color
touch screen and intuitive interface
 Consistent results — robust design ensures effective and
consistent performance run to run
 Easy optimization — this gradient thermal cycler allows fast PCR
optimization using a unique thermal gradient
 Easy protocol management — protocols can be organized using
personalized folders or a USB flash drive
 Small space-saving footprint — the T100 is a compact thermal
cycler that fits in any laboratory

Applications and Uses for the T100 Thermal Cycler

 Nucleic acid amplification (PCR)

 Gene cloning and analysis
 Gene expression analysis
 Mutational analysis
 Cycle sequencing
Spesification Sample capacity 96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x
96-well plate
Maximum ramp 4
rate, °C/sec
Avarage 2.5
Temperature range 4–100°C
Temperature accuracy ±0.5°C of programmed target
Temperature ±0.5°C well-to-well within 30 sec of arrival
uniformity at target temperature
Input power 100–150 VAC, 50–60 Hz; 220–240 VAC,
50–60 Hz; 700 W maximum
Display 5.7'' VGA color touch screen
Port 1 USB A
Memory 500 typical programs; unlimited with USB
flash drive expansion
Dimensions (W x D x 26 x 47 x 23 cm (10 x 18 x 9'')
H)
Weight 9 kg (20 lb)
Gradient range 30–100°C
Temperature 1-25°C
differential range
Price $4,995

2. The CFX Connect Real-Time PCR Detection System

Image

Description The CFX Connect Real-Time PCR Detection System offers

two-target analysis, excellent thermal cycler specifications, and the
same reliable performance as the CFX96 Touch™ Real-Time PCR
Detection System. The system incorporates innovative optical
technologies with powerful software to provide maximal reliability
and efficiency for all your real-time PCR needs.
Key Features and Benefits
The CFX Connect Real-Time PCR Detection System offers:

 Easy startup — obtain great results right away with

factory-calibrated dyes, quick setup, and intuitive software
 Effortless optimization — save time and reduce costs by
optimizing assays in a single run using the thermal gradient
 Powerful data analysis tools — quickly and accurately validate
and analyze data with the advanced analysis modules of CFX
Maestro™ Software
 Accelerated publication submission — include MIQE
annotations and generate RDML files using Biogazelle's qbase+
Software

Spesification Chassis CFX Connect

Maximum ramp 5
rate, °C/sec
Average ramp 3.3
rate, °C/sec
Heating and cooling Peltier
method
Lid, °C Heats up to 105
Temperature
Range, °C 0–100
Accuracy, °C ±0.2 of programmed target at 90°C
Uniformity, °C ±0.4 well-to-well within 10 sec of arrival
at 90°C
Gradient
Operational range, °C 30–100
Programmable span, °C 1–24
Optical Detection
Excitation 3 filtered LEDs
Detection 3 filtered photodiodes
Range of 450–580
excitation/emission
wavelengths, nm
Sensitivity Detects 1 copy of target sequence in
human genomic DNA
Dynamic range 10 orders of magnitude
Scan Time
All channels, sec 12
FAM/SYBR® Green 3
only, sec
Software
Operating systems Windows 7, Windows 8, Windows 10
Multiplex analysis Up to 2 targets per well
System
Licensed for real-time Yes
PCR
Sample capacity, wells 96
Sample size, µl 1–50 (10–25 recommended)
Communication interface USB 2.0
Electrical approvals IEC, CE
 Dimensions (W x L x H), 33 x 46 x 36/13 x 18 x 14
cm/in
Weight, kg/lb 21/47
Price $17,995

3. Benchmark Accuris myGel Mini Electrophoresis system

Image

Description The myGel mini gel box attaches directly to the power supply for a
secure connection every time. Selectable parameters include 3 preset
voltages - 35V, 50V or 100V - and a 99-minute timer. All are set
using the membrane keypad. The safety lid is vented to help
dissipate heat generated during electrophoresis and features a
viewing area above the gel. Current will only run through the
system when the lid is properly in place on top of the gel box. Trays
and casting stands for both 10.5 x 6cm and 5 x 6cm gels are
included with the myGel Mini system, as are double-sided combs.
The smaller tooth combs are compatible with multichannel pipettes.
Casting is quick and easy - just place the tray in the casting stand
and pour. The trays feature a dark contrast strip to help visualize
wells while loading and lines that fluoresce under UV and blue light
to track the progress of the run.
Benchmark Accuris myGel Mini Electrophoresis System features
and benefits:

 Includes: gel box, safety lid, power supply, two 1.5 x 6 cm and
 four 5 x 6 cm gel trays, casting stands for gel trays and two
double-sided combs for each gel size
 Smaller tooth combs compatible with multichannel pipettes
 Power supply connects to gel box
 Selectable parameters: 35V, 50 V and 100
 0-99 minutes or continuous timer
 Vented, clear safety lid dissipates heat generated during
electrophoresis
 Unit runs when lid is securely in place
 Dark contrast strip on tray helps visualize wells when loading
 Two-Year Manufacturer Warranty

Spesification Dimensions 7.5W x 5.1D x 2.1H in (190.5 x 129.54 x 53.34

mm)
Electrical 115V, 50-60 Hz/230V, 50-50Hz
Output Voltage 35V, 50V or 100V
Timer 0-99 minutes or continuous
Max. Buffer 230 mL
Volume
Gel Capacity One 10.5 x 6 cm or Two 5 x 6 cm
Weight 1 lb. (.45 kg)
Safety Magnetic and mechanical lid interlock
Price $495

4. Eppendorf 5424 Centrifuge

Image

Description The Eppendorf 5424 R Centrifuge is a fast, accurate, and

durable centrifuge which satisfies any application. With their
24-place capacity and speed up to 21,130 × g they are perfectly
equipped for all modern molecular biology applications in
Eppendorf and PCR tubes. When working with spin columns kits,
the Eppendorf Kit rotor® with raised rim solves the issue of open
tube caps shearing off during the elution step. The refrigerated
Eppendorf 5424 R Centrifuge features state of the art cooling
technology to ensure energy efficiency and high-temperature
accuracy for maximum sample protection. For ease-of-operation and
time savings, the Eppendorf 5424 R Centrifuge cools down to 4 °C
in only 8 minutes.

Eppendorf 5424 R Centrifuge features and benefits:

 Max. rotor capacity: 24 × 1.5/2.0 mL vessels

 Max. speed: 20,238 × g (14,680 rpm)
 OptiBowl® design for quiet operation, even without a rotor lid
 Soft-touch one-finger lid closure for ergonomic operation

 SOFT brake to protect sensitive samples

 Full digital display with time and speed display
 Separate short-spin key for quick centrifugation
 Automatic lid opening at the end of the run – prevents sample
warming and allows easy access to samples (only Eppendorf 5424
Centrifuge)
 Certified aerosol-tight rotor comes standard for safe centrifugation
of hazardous samples
  Available as a rotary knob version for fast parameter selection, or
keypad version for easy cleaning
 Refrigerated Centrifuge 5424 R Features:
o Temperature range from -10 °C to +40 °C
o Guaranteed temperature of 4 °C at maximum rotational speed
o FastTemp function for quick pre-cooling of the centrifuge, e.g.
from ~ 21 °C to 4 °C in only 8 minutes

Spesification Max g-force 21,130 rcf

Speed range 100-15,000 rpm
Capacity 24 x 1.5/2.0 mL tubes
Number of rotors 4
Capacity for 1.5 24
mL tubes
Refrigeration Yes
Temperature -10°C to 40°C
control range
Power supply 120 V, 50/60 Hz
Acceleration time 15 s (in FA-45-24-11)
to max speed
Braking time from 16 s (in FA-45-24-11)
max speed
Acceleration/Brak 2
e speed settings
Timer 30 s to 9:59 h, with cont. mode
Noise level <54 dB(A)
Dimensions (W x 20 in / 51 cm
D x H)
Weight 46.3 lb / 21 kg
Price $5,983

5. ZOE Fluorescent Cell Image

Image

Description The ZOE Fluorescent Cell Imager eliminates the complexities

of cell imaging associated with traditional microscopes. This
fluorescence imaging system combines the ease of use of a personal
tablet with the power of an inverted microscope.
An Android-based platform, the ZOE Cell Imager uses an
intuitive touch-screen interface to control the brightfield, three
fluorescence channels, and the integrated digital camera.
The ZOE Fluorescent Cell Imager is a complete digital
imaging system, allowing users to view samples, capture and store
images, and create multicolor overlays. Thanks to the built-in light
shield, the ZOE Cell Imager does not require a darkroom for
fluorescence imaging.

Features and Benefits of the ZOE Fluorescent Cell Imager

 Simplified cell imaging — the intuitive touch-screen interface

allows users to view cells, capture images, and create
multichannel merges with minimal training
 Flexible operation — brightfield and three fluorescence channels
enable use for routine cell culture applications and more
sophisticated imaging applications
 Fluorescence imaging at your bench — light shield permits
fluorescence imaging in ambient light
 Robust construction — fully integrated system with long-life
LEDs, ready for intensive daily use
 LED light sources — thousands of hours of illumination that are
instantly ready after power-on
 Large viewing area — the motorized stage and wide field of
view allow you to see more of your sample, faster
 Small footprint — compact size accommodates crowded lab
benches

Applications of the ZOE Fluorescent Cell Imager

 Use the ZOE Cell Imager to check/screen samples prior to
high-content analysis (HCA), high-throughput screening (HCS),
confocal imaging, or fluorescence-activated cell sorting (FACS).
With a brightfield and three fluorescent channels, the ZOE Cell
Imager has all the features needed for daily cell culture work as well
as fluorescent applications:

 Visual estimation of cell confluency

 Observation of general cell health and morphology
 Cell growth and proliferation monitoring
 Capturing cell images (with or without fluorescent labels)
 Visualization of expressed fluorescent proteins
 Immunofluorescent protein localization
 Estimation of transfection efficiency

Illumination Light Sources

 Blue channel uses a UV LED

 Green channel uses a blue LED
 Red channel uses a green LED
 Brightfield channel uses a ring of multiple green LEDs for
reduced chromatic aberration

Spesification Imaging channels Brightfield channel and 3 fluorescence

channels (blue, green, and red)
Light source Blue channel: UV LED
Green channel: blue LED
Red channel: green LED
Brightfield channel: multiple green LEDs
(reduces chromatic aberration)
User interface 10.1 in. color (26 cm) touch-screen LCD
monitor, with anti-glare and anti-fingerprint
treatment, 1,280 x 768 pixel image resolution,
80–180° angle tilt range
Focusing Coarse and fine, manual adjustment
mechanism
Camera Monochrome camera, 12 bit CMOS, 5
megapixels
Data format JPEG, TIFF, or RAW image files
Image merge Images from up to 4 channels can be overlaid
Data storage 16 GB internal memory (~2,500 JPEG
files,1,500 TIFF files, 400–800 RAW files)
Data export Yes, 2 USB ports
 Display output Yes, 1 HDMI port
Objective 20x
Numerical 0.40
aperture
Display Standard: 175x; zoom: 700x
magnification
Maximum 0.70 mm2 field of view
imaging area
Motorized stage 6 mm travel in X, Y direction, touch-screen
control of travel speed and direction
Compatible with Flasks: T25, T75, or T225
Multiwell plates: 6-, 12-, 24-, 48-, 96-, or
384-well microplates
Dishes: 35 mm, 60 mm, or 100 mm
Slides: chamber slides or standard glass
microscopy slides
Software Stand-alone Android operating system; PC is
not required for operation
Instrument size (L 33 x 32 x 30 cm (13 x 12.6 x 11.6 in.)
x W x H)
Instrument weight 9 kg (19.7 lb)
Price Inquire

6. ZE5 Cell Analyzer

Image

Description The ZE5 Cell Analyzer is the first flow cytometer with a fully
integrated and flexible loader that can accommodate any sample,
tube, or rack, complete with temperature control and vortexing. The
sample delivery system allows maximized sample usage efficiency
by returning unused sample to a well or tube. The ZE5 has
unparalleled speed that can detect up to 100,000 events per second
with no aborts. The ZE5 offers high parameter capabilities with up
to 30 parameters, 5 lasers, and dual FSC options that allow for small
particle detection.
Everest™ Software offers user-friendly features for setup,
such as the Spectral Viewer for experiment design and QC checks
with the ZE5 EYE that ensures filters are in the correct position
prior to analysis.
The ZE5 Analyzer was created by gathering input from leading flow
cytometry experts across the world and was designed to address
user-reported gaps in currently available systems.

Capable of High Parameter & Complex Experiments

 Up to five spatially separated lasers ranging from 355 nm to 640

nm
 Up to 27 fluorescence detectors
 Dual forward scatter design allows standard FSC detection while
simultaneously collecting small particle or alternate wavelength
scatter data
 Up to 100,000 events per second reduces analysis time for rare
cell detection

Flexible Loading Options Enable True Walk-Away

Functionality

 Up to 384 well plates for high-throughput capacity

 Single tube loading for stat samples
  Temperature-controlled sample loading for sensitive samples
 Sample agitation on board to keep cells in suspension

Spesification Fluorescence <100 MESF for FITC, PE, and APC

sensitivity
Scatter sensitivity <0.3 μm FSC resolution with small-particle
detection module
Sample loading Standard microplates (127.75 x 85.5 mm): up
to 384-well
5 ml tubes (12 x 75 mm): stat tube position for
single tube, 40-tube rack
Sample <15 min for 96-well plate in high-throughput
throughput mode
Excitation Up to five spatially separated lasers. Standard
sources options include:
355 nm, 50 mW
488 nm, 100 mW
640 nm, 100 mW
405 nm, 100 mW
561 nm, 50 mW
Detection Up to 30 detectors, including FSC and SSC;
optional second FSC detector
Cuvette Fused silica with 145 x 265 μm channel
Speed Up to 100,000 events per second with all
parameters enabled
Data processing Simultaneous measured peak, area, and width
for every channel
24-bit data for peak and area
17-bit data for width with high-resolution linear
interpolation at the half height
Fluidics Bulk fluids: 4 x 4 L bulk fluid tanks on board
for sheath and waste
Onboard additive concentrate and cleaner
Operational flow 0.025–3.5 μl/sec
rates
Dimensions (W x 74 x 69 x 66 cm (29 x 27 x 26 in)
D x H)
Weight 110 kg (240 lb) maximum
Data format Flow Cytometry Standard (FCS) 3.1
Price Inquire

7. TC20 automated cell counter

Image

Description The TC20 automated cell counter counts mammalian cells in one
simple step using its innovative auto-focus technology and
sophisticated cell counting algorithm to produce accurate cell
counts in less than 30 seconds. Upon insertion of a counting slide,
the TC20 cell counter rapidly provides a total cell count (with or
without trypan blue staining) and assesses cell viability via trypan
blue exclusion.
Key Features and Benefits

 Compatible with a broad range of cell sizes and types —

counts cell lines, primary cells (from tissue or blood), and stem
cells
 Innovative auto-focus technology — removes the variation
associated with manual focusing and leads to precise cell counts
in 30 seconds
 Cell size gates — user selects a population of interest in complex
samples, such as primary cells, or lets the cell counting algorithm
do all the work
 Cell viability — analyzes cells accurately using multifocal plane
analysis
 Easy to archive and analyze — stores up to 100 counts in the
onboard memory for access any time, or use the optional TC20
data analyzer software on your PC to further analyze exported cell
images

Getting all the data you need about your cell cultures is fast and
easy; the TC20 cell counter and disposable counting slides eliminate
 the need for tedious setup, cleaning, or maintenance. The TC20 cell
counter is simple and intuitive to use — learn to use the TC20
automated cell counter in the introduction video or take a virtual test
drive.
Spesification Counting time, 30
sec
Cell concentration 5 x 104–1 x 107
range, cells/ml
Optimal cell 1 x 105–5 x 106
concentration
range, cells/ml
Cell diameter 6–50
range, µm
Sample volume, 10
µl
Data storage, 100
counts
Data export Via USB flash drive
Printer Yes
connectivity
Dilution Yes
calculator
Image of cells Yes
available
Dimensions (W x 9 x 15 x 25.4 (7.5 x 6 x 10)
D x H), cm (in)
Weight without 2.2 (4.8)
the external power
supply, kg (lb)
Price Inquire

8. Eppendorf Galaxy 170 Stackable CO2 Incubator

Image

Description These models can be conveniently placed on or under the bench or

even double-stacked, making them ideal for a wide range of
specialized applications. Galaxy 170 R models provide a tremendous
amount of usable space that can be optimized with options such as
High Temperature Disinfection, O2 control, and split inner doors.
Galaxy 170 R incubators feature an integrated controller for simple
on-screen and data logging.
Features and Benefits:

Standard features:
o Sealed inner glass door
o Reliable IR CO2 sensor
o RS-232 communication port
o 25 mm access ports
 Two O2 control options (0.1 – 19 %, 1 – 19 %) for hypoxic
studies, also available with split inner doors
 Further Options:
 o High Temperature Disinfection (4 hour at 120 °C)
o Copper chamber and interior for optimized protection
against contamination
o Humidity alert package
 Fanless design offers more usable space and eliminates the fan,
an often repeated source of contamination
 Six-sided direct heating system provides a uniform incubated
environment to gently bathe cells
 Deep-drawn, stainless steel chamber eliminates seams or welds
and allows easy cleaning
 Low gas consumption
 Alarm relay standard

Spesification CO2 control ±0.1 %

CO2 range 0.2 – 20 %
CO2 stability at ±0.2 %
5 % CO2
CO2 uniformity ±0.1 %
Display readout Temperature and CO2 level, 72-hour
data/chart logger, alarm settings
In-line gas regulator Standard
Maximum Number of 8
shelving racks
Capacity 170 L
Power supply 120 V, 50/60 Hz
Dimensions 70.8 × 71.1 × 84.5 cm
(W × D × H)
Weight w/o 89.9 kg
accessories
Temperature range Ambient +4 °C to 50 °C
Price $7,130
9. Labnet Mini Digital Dry Bath
Image

Description A broad temperature range, from ambient to 150 ºC, makes the
Labnet Digital Dry Baths useful for a variety of applications in
molecular biology, histology, clinical, environmental and industrial
laboratories. Although the price of these baths is comparable to
analog models, they feature microprocessor control and digital
setting/display of temperature. Digital performance at analog prices.
Labnet Dry Bath features and benefits:
  Temperature range from 0 to 150 ºC
 Digital display and setting
 Microprocessor controlled
 broad application range
 Computer connectability
 Variety of block attachments available

Spesification Temperature Ambient +5º to 150ºC, in 0.1º increments

range
Temperature 0.1ºC
resolution
Temperature ±0.2ºC
uniformity
Temperature ±0.3ºC
accuracy
Temperature Microprocessor
control
Block chamber Stainless steel
construction
Dimensions (W x 7.9 x 10.4 x 3.3 in (20 x 26.5 x 8.3 cm)
D x H)w
Weight 4.8 lb/2.2 kg
Electrical 230V~ or 120V~, 50/60 Hz
Price $273

10. Eppendorf Research Plus

Image

Description Benefits of the Eppendorf Research plus

 Ultra lightweight. Made of Forton®, an organic polymer which

is resistant to heat, mildew, bleaches, acids and alkalies,
sunlight, aging, and abrasion.
 Ergonomically placed control buttons. Control buttons are
placed in user-friendly position to minimize user exertion.
 Low tip ejection force. The Eppendorf Research plus tip
ejection force can be as low as only 3.6 N.
 Completely flexible. The lower part of the Research plus is
autoclavable and can be removed easily.
 Variety of choices. Available in single channel, multi-channel
(8- and 12- channel), and fixed-volume options.
 Easy-to-read volume display. Displays in 4 digits and has a
magnifying shape.

The Research Plus Pipette reduces risk of RSI with spring loaded
tip cones
Designed to alleviate stress on the users hand when mounting the
pipette tip, the Research Plus absorbs the pressure the user is
applying when mounting the tip. Thus, you never have to jam the
 pipette onto the tip again.
Certified Refurbished - Keep the Quality, Ditch the Price
We also offer refurbished pipettes - certified for quality by an
in-house lab equipped for calibration, preventative maintenance, and
repair. We have the Eppendorf Research Plus Pipette as a single and
multichannel refurbished pipette.
Spesification

Price $913