The expertise in culture media

preparation and distribution

PREPARATION
MEDIA PREPARATION IN THE
WORKFLOW
MEDIA PREPARATION IN THE
WORKFLOW
 MEDIA PREP IN THE WORKFLOW

Media preparation

Masterclave
Sample preparation

Alternative method Reference method

Plate Tubes

APS One PMi / XY500

Media Preparation
 MANUAL MEDIA PREPARATION

STERILITY A high temperature enough to sterilize the media

°C-15min, 110°
(Ex.: 121° °C-20min)

FERTILITY But a temperature not too high not to burn the

media (not to destroy sugar, pepton,…)
ex: <121,5 or 122°°C. ex Malt agar

REPEATABILITY The insurance to always have the same media

Homogeneity and temperature accuracy

 MANUAL MEDIA PREPARATION

1. Weighing dry media 2. Stirring: dry media

Drawbacks: + water at 100°C

°C
Risk of burn with agar at 100°

Glass handling:
Risk of cut

Time consuming ~ 4min/ bottles (1 bottle = 1 sample)

No traceability
 CLASSIC MEDIA PREPARATION

3. Autoclaving bottles
Drawbacks:

No accurate temperature control (+/- 3°C)

Temperature different for top/base

No homogeneity of the temperature (no stirring in the bottle)

MEDIA PREPARATION

Masterclave 528
(5-28 litres)

Masterclave 60
(10-60 litres)

Masterclave 09
(1-9 litres)
 MASTERCLAVE PRINCIPLE

Core temperature by a temperature probe

controller inside the media

Rapid cooling by a
water cooling
device

Homogeneous
temperature by
large paddle stirrer
Masterclave 60

Homogeneity and temperature accuracy

 MASTERCLAVE KEY POINT

STANDARDIZATION
The cycle parameters controlled by microprocessor ensure
repeatability of each media preparation.
(heating, sterilization, cooling, dispensing).

40 free programs
 MASTERCLAVE KEY POINT

PRODUCTIVITY
From 1 to 60 liters in one batch

Time saving : no more manual stirring or autoclaving

(10min to run a cycle)

Money saving in comparison with ready-to-use

solutions.
Additional benefits :

Possibility to prepare all types of media or diluent

 MASTERCLAVE KEY POINT

SIMPLICITY
Easy handling : adding powder + distilled water and starting cycle.
(10 min/cycle)

Intuitive and simple graphic screen

Easy programming.

No need to supervise the autopreparator : walk-away device

Additional benefits:
Less cleaning bottles operation (ecological)
 MASTERCLAVE KEY POINT

QUALITY
Insurance to have fresh media everyday.

Media comply with Quality Insurance and GLP

metrology and traceability guarantee the quality of diluent

Better control of media temperature within the auto-preparator

than in autoclave (+/- 1°C)
 MASTERCLAVE KEY POINTS

SECURITY
In compliance with the EC standards, this equipment is equipped with
various safety devices to ensure safe operation for the operator

Safety devices:
protection cover locking
safety valve
thermal cut off switch

Several alarms:
temperature variation error
short circuit or breakage of the probe
 MASTERCLAVE KEY POINT

TRACEABILITY
A printer can be connected to ensure a total
traceability for quality control purposes:
- operator’s name
-batch number
-program used
-date/time
-cycle curve

-
 MASTERCLAVE KEY POINT

TRACEABILITY
 MASTERCLAVE CYCLE

Sterilization

Dispensing

Temperature

Time
 MASTERCLAVE CYCLE

Sterilization

Step

Dispensing

Temperature

Time
 MASTERCLAVE CYCLE

Step 2

Step 3
Step 1 Dispensing

Temperature

Time
Addition
Addition Addition
 MASTERCLAVE KEY POINT

STANDARDIZATION

PRODUCTIVITY

SIMPLICITY

QUALITY

SECURITY

TRACEABILITY
MASTERCLAVE KEY POINT
 MASTERCLAVE 09

LID VESSEL

Light and removable vessel

enables to prepare 1 to 9 liters of media

Large removable magnetic stirrer

Easy single opening
and closing handle INTERFACE

Temperature TRACEABILITY
probe inside the
Optional ticket printer
media

External temperature probe

Easy access to
sterile media by a LCD screen for an easy programming for an external temperature control
cap on the lid
Alpha numerical keypad
 MASTERCLAVE 09

Capacity: 1to9L

Preparation time:
45min for 1 liter (sterilization 121°C, 15min)

Dimensions
w x h x d= 500 x 435 x 590mm

Installation
Electrical connection: 230V, 16A

Water supply:
Maximum hardness: 7°TH
Temperatures: 6 to 20°C
Pressure: 1 to 3 bar
Minimum flow: 6liters per minute
 MASTERCLAVE 528
LID INTERFACE
Easy single opening
and closing handle
Easy access to sterile
media by a cap on the
lid
Grpahic screen for easy
programing
Clear visualisation of settings
VESSEL and the temperature curve
Status indicator lights and beeps
inform the operator

TRACEABILITY
Optional printer
Double skin vessel CLEANING Connection to PC
(5 to 28 liters of media)
Temperature probe at the bottom MOBILITY
of the vessel
Designed to be mobile
Hole for a second temperature Easy cleaning with gate to drain
probe (external control) vessel Four larges wheels
Removable magnetic stirrer Access protected by a safety Two larges handles
hatch protection against shocks
Stirring speed may adjustable
 MASTERCLAVE 528

Capacity: 5 to 28L
MAIN APPLICATIONS
Combine system for contract labs:
Preparation time: 28 liters of diluent in the morning
90 min for 28liters of media (121°C/15min) 5-10 liters of agar in the afternoon

Medium autoprep for manufacturer

Dimensions
w x h x d= 760 x 1100 x 580mm ECO MODE
• Fast or Eco mode for
saving cooling water or
Installation time
• Eco mode for stop
Electrical connection: printing once in
400V triphase with neutral, 16A per phase dispensing phase.
OR
230V triphase without neutral 20A per phase
Water supply:
Maximum hardness: 7°TH
Temperature: 6 to 20°C
Pressure: 1 to 3 bar
Minimum flow: 6liters per minute
 MASTERCLAVE 60

LID INTERFACE

Effort less opening

Lid fixed on arm lifted
by a gas jack

VESSEL
Graphic screen for easy
programing
Clear visualization of settings
and the temperature curve
Beeps inform the operator

Double skin vessel

(20 to 60 liters of media) TRACEABILITY
Temperature probe at the bottom PRIMING Built-in printer
of the vessel Connection to PC
Hole for a second temperature Priming by an anti-retention
probe (external control) range gate at the bottom of the MOBILITY
vessel.
Removable magnetic stirrer
OPTION: priming by the lid Designed to be mobile
Stirring speed may adjustable Four large wheels
Two large handles
 MASTERCLAVE 60

Capacity: 10 TO 60L
MAIN APPLICATION
Preparation time: - Agar for agar manufacturer
120 min for 60 liters of media (121°C/15min) - Diluent for labs

Dimensions
w x h x d= 1,03 x 0,65x 1,10m

Installation
Electrical connection:
400V triphase with neutral, 20A per phase
OR
230V triphase without neutral 32A per phase
Water supply
Maximum hardness: 7°TH
Temperatures: 6 to 20°C
Pressure: 1 to 3 bar
Minimum flow: 6liters per minute
 PREPARE & DISPENSE MEDIA

PREPARE MEDIA DISPENSE MEDIA

Masterclave

Agar in plate
APS One

Sample
dilution Dilumat

Broth in tube PMi + XY500

MEDIA PREPARATION
 MEDIA DISPENSING MANUAL METHOD

1. Autoclaving bottles 2. Keeping in water bath 3. Manual dispensing

MEDIA DISPENSING KEY POINT
 MEDIA DISPENSING KEY POINT

ACCURACY
accuracy of dispensed volume +/- 0,25mL (<20mL)
automatic calibration

QUALITY
work in compliance with GLP
sterile environment for the dispensing
combined with Masterclave: dispensing at low temperature
reduce condensation
rapid cooling

AUTONOMY
Complete walk away system productivity

SPEED
750 plates/hour (15mL)
650 plates/hour (18mL)
MEDIA DISPENSING KEY POINT
 APS ONE KEY POINT

SIMPLICITY
User friendly interface:
• Big colour screen
• Intuitive display (icon)
Direct access to main application

Quick loading (5 by 5 stacks)

Easy to load: reduced plate stacking hight

Optimal access for easy cleaning

MEDIA DISPENSING LABAUTO SYSTEM

PMi

APS One

XY500
DISPENSING MEDIA IN TUBE
 DISPENSING MEDIA : PMi

ISO COMPLIANT EASY TO USE SMART

ISO Compliant: Large touch screen 4 different tubing:
• 7218 • 1.6mm
• 6887-1 • 3.2mm
• 16189 Easy to program • 4.8mm
• 6.4mm
Dispensing accuracy:
1% accuracy ≤ 225ml LED status indicator Wide range of volume:
(to help manipulator) • From 2ml to 20L
Up to 20L (2% accuracy)
Speed dispensing:
• Up to 600 rpm
 DISPENSING MEDIA IN TUBE

PMi XY500
Semi-automatic Complete walk away system

Automation and volume accuracy

DISPENSING MEDIA IN TUBE
 DISPENSING MEDIA : XY500

PRODUCTIVITY EASY TO USE SMART

1500 tubes/hour (9ml) Easy tube Loading with All kind of containers:
optional stands Bottles
Vials
250 bottle/hours (225ml) Tubes

4 differents 96 tubes
Large capacity: rack
up to 500 tubes in one cycle Easy to program
Step by step program Flexible loading

Walk away system

DISPENSING MEDIA : Pmi/XY500
Pmi/XY500
The expertise in culture media preparation and distribution - DISPENSING

XY500 FEATURES

CAPACITY MOTOR
• 504 tubes in one cycle The step by step motor allows a very big
• Speed: 1000 tubes/hour (9mL) accuracy for the dispensing
• Maximum height : 210 mm In case of of arm jamming, once the problem
solved, the cycle starts exactly from where it
stopped.
APPLICATION
For dispensing liquid (sterile or not) in tubes, INTERFACE
bottles, Erlen Meyer… Numerical
keyboard and
LCD screen
allows a simple
DIMENSIONS programming
• L x P x H = 710 x 650 x 450 mm with storage of
the rack
• Weight : 25kg
configurations

WALK AWAY SYSTEM

Complete walk away system, no
ERGONOMY
need to supervise it Different types of rack is available
(96 to 252 tubes)

Easy tubes load,

Developped by AES
The expertise in culture media preparation and distribution - DISPENSING

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