3 (campuran).

exp 09/21/18-21:12:14 1/7

< Experiment title >

Name :

User :

Note :

Date : 09/21/18 Time : 21:12:14

< Protocol parameters >

Experiment file path : new.exp

Measurement type : End point

Measurement mode : Continuous

Main_1 filter(nm) : 600

Starting method : Immediate

Need shake : Yes

Shake speed : Low

Shake time(sec.) : 3

Need incubate : No

Need quantitative : Yes

Quant. method : Curve on plate

Quant. standards number : 8

Quant. replicates number : 2

Curve fit method : 4 param logistic

Need Cutoff : No

Need ratio/inhibition : No

< Plate layout >

1 2 3 4 5 6 7 8 9 10 11 12

A STD08-1 STD08-2 SAM01-1 SAM01-2 SAM09-1 SAM09-2 SAM17-1 SAM17-2 ... ... ... ...

B STD07-1 STD07-2 SAM02-1 SAM02-2 SAM10-1 SAM10-2 SAM18-1 SAM18-2 ... ... ... ...

C STD06-1 STD06-2 SAM03-1 SAM03-2 SAM11-1 SAM11-2 SAM19-1 SAM19-2 ... ... ... ...

D STD05-1 STD05-2 SAM04-1 SAM04-2 SAM12-1 SAM12-2 SAM20-1 SAM20-2 ... ... ... ...

E STD04-1 STD04-2 SAM05-1 SAM05-2 SAM13-1 SAM13-2 SAM21-1 SAM21-2 ... ... ... ...

F STD03-1 STD03-2 SAM06-1 SAM06-2 SAM14-1 SAM14-2 SAM22-1 SAM22-2 ... ... ... ...

G STD02-1 STD02-2 SAM07-1 SAM07-2 SAM15-1 SAM15-2 ... ... ... ... ... ...

H STD01-1 STD01-2 SAM08-1 SAM08-2 SAM16-1 SAM16-2 ... ... ... ... ... ...

< Raw abs. matrix >

P1 1 2 3 4 5 6 7 8 9 10 11 12

A 1.303 1.276 0.739 0.775 0.925 0.641 0.582 0.858 ... ... ... ...

B 1.184 1.222 1.054 0.970 0.451 0.558 0.717 0.804 ... ... ... ...

C 1.183 1.118 0.639 0.569 1.288 1.540 0.718 0.918 ... ... ... ...
 3 (campuran).exp 09/21/18-21:12:14 2/7

D 1.115 0.710 0.723 0.284 0.552 0.782 0.589 0.684 ... ... ... ...

E 0.895 0.933 1.034 0.839 1.553 1.430 1.341 0.476 ... ... ... ...

F 0.599 0.705 0.744 0.640 0.434 1.207 0.378 0.270 ... ... ... ...

G 0.495 0.474 0.679 0.596 0.791 0.770 ... ... ... ... ... ...

H 0.320 0.314 0.592 0.753 0.905 0.709 ... ... ... ... ... ...
 3 (campuran).exp 09/21/18-21:12:14 3/7

< Calibration >

Calib. curve

Name Meas. Conc. Fit type : 4 param logistic

C01 STD01 0.320 Meas. scale : linear

0.314 Conc. scale : linear

0.317 25.000

C02 STD02 0.495

0.474 Abs.

0.485 125.000 2

C03 STD03 0.599

0.705

0.652 250.000

C04 STD04 0.895

0.933

0.914 500.000

C05 STD05 1.115

0.710

0.913 750.000

C06 STD06 1.183 0

1.118 0 2100

1.151 1000.000 Formula : Conc.

C07 STD07 1.184 Abs = (a0-a3)/(1+power(C/a2,a1))+a3

1.222 a0 : 0.2588

1.203 1500.000 a1 : 0.9851

C08 STD08 1.303 a2 : 612.1

1.276 a3 : 1.608

1.290 2000.000 R2 : 0.9845

Residuals table

C(set) Abs. C(cal) Residual

C01 25.000 0.317 26.319 1.319

C02 125.000 0.485 119.691 -5.309

C03 250.000 0.652 248.414 -1.586

C04 500.000 0.914 577.479 77.479

C05 750.000 0.913 574.010 -175.990

C06 1000.000 1.151 1203.498 203.498

C07 1500.000 1.203 1445.956 -54.044

C08 2000.000 1.290 2013.128 13.128

 3 (campuran).exp 09/21/18-21:12:14 4/7

Conc. matrix

1 2 3 4 5 6 7 8 9 10 11 12

A 2013.128 2013.128 355.493 355.493 386.315 386.315 314.804 314.804 ... ... ... ...

B 1445.956 1445.956 776.466 776.466 132.890 132.890 358.951 358.951 ... ... ... ...

C 1203.498 1203.498 207.096 207.096 3747.813 3747.813 431.076 431.076 ... ... ... ...

D 574.010 574.010 132.219 132.219 262.214 262.214 234.176 234.176 ... ... ... ...

E 577.479 577.479 617.025 617.025 6690.173 6690.173 567.132 567.132 ... ... ... ...

F 248.414 248.414 286.245 286.245 433.756 433.756 29.700 29.700 ... ... ... ...

G 119.691 119.691 235.051 235.051 382.658 382.658 ... ... ... ... ... ...

H 26.319 26.319 266.915 266.915 416.579 416.579 ... ... ... ... ... ...

< Data list >

SAMPLES

Name Well Replicate Abs. SD CV% Conc. Unit Cutoff R/I(%)

SAM01 A3 1 0.739 ........ ........ ........ ........ N/A ........

SAM01 A4 2 0.775 ........ ........ ........ ........ N/A ........

SAM01_avg ........ ........ 0.757 0.018 2.38 355.493 ug/mL N/A N/A

SAM02 B3 1 1.054 ........ ........ ........ ........ N/A ........

SAM02 B4 2 0.970 ........ ........ ........ ........ N/A ........

SAM02_avg ........ ........ 1.012 0.042 4.15 776.466 ug/mL N/A N/A

SAM03 C3 1 0.639 ........ ........ ........ ........ N/A ........

SAM03 C4 2 0.569 ........ ........ ........ ........ N/A ........

SAM03_avg ........ ........ 0.604 0.035 5.79 207.096 ug/mL N/A N/A

SAM04 D3 1 0.723 ........ ........ ........ ........ N/A ........

SAM04 D4 2 0.284 ........ ........ ........ ........ N/A ........

SAM04_avg ........ ........ 0.504 0.220 43.59 132.219 ug/mL N/A N/A

SAM05 E3 1 1.034 ........ ........ ........ ........ N/A ........

SAM05 E4 2 0.839 ........ ........ ........ ........ N/A ........

SAM05_avg ........ ........ 0.937 0.098 10.41 617.025 ug/mL N/A N/A

SAM06 F3 1 0.744 ........ ........ ........ ........ N/A ........

SAM06 F4 2 0.640 ........ ........ ........ ........ N/A ........

SAM06_avg ........ ........ 0.692 0.052 7.51 286.245 ug/mL N/A N/A

SAM07 G3 1 0.679 ........ ........ ........ ........ N/A ........

SAM07 G4 2 0.596 ........ ........ ........ ........ N/A ........

SAM07_avg ........ ........ 0.638 0.042 6.51 235.051 ug/mL N/A N/A

SAM08 H3 1 0.592 ........ ........ ........ ........ N/A ........

SAM08 H4 2 0.753 ........ ........ ........ ........ N/A ........

SAM08_avg ........ ........ 0.673 0.081 11.97 266.915 ug/mL N/A N/A

SAM09 A5 1 0.925 ........ ........ ........ ........ N/A ........

SAM09 A6 2 0.641 ........ ........ ........ ........ N/A ........

SAM09_avg ........ ........ 0.783 0.142 18.14 386.315 ug/mL N/A N/A
 3 (campuran).exp 09/21/18-21:12:14 5/7

SAM10 B5 1 0.451 ........ ........ ........ ........ N/A ........

SAM10 B6 2 0.558 ........ ........ ........ ........ N/A ........

SAM10_avg ........ ........ 0.505 0.054 10.6 132.890 ug/mL N/A N/A

SAM11 C5 1 1.288 ........ ........ ........ ........ N/A ........

SAM11 C6 2 1.540 ........ ........ ........ ........ N/A ........

SAM11_avg ........ ........ 1.414 0.126 8.91 3747.813 ug/mL N/A N/A

SAM12 D5 1 0.552 ........ ........ ........ ........ N/A ........

SAM12 D6 2 0.782 ........ ........ ........ ........ N/A ........

SAM12_avg ........ ........ 0.667 0.115 17.24 262.214 ug/mL N/A N/A

SAM13 E5 1 1.553 ........ ........ ........ ........ N/A ........

SAM13 E6 2 1.430 ........ ........ ........ ........ N/A ........

SAM13_avg ........ ........ 1.492 0.062 4.12 6690.173 ug/mL N/A N/A

SAM14 F5 1 0.434 ........ ........ ........ ........ N/A ........

SAM14 F6 2 1.207 ........ ........ ........ ........ N/A ........

SAM14_avg ........ ........ 0.821 0.387 47.11 433.756 ug/mL N/A N/A

SAM15 G5 1 0.791 ........ ........ ........ ........ N/A ........

SAM15 G6 2 0.770 ........ ........ ........ ........ N/A ........

SAM15_avg ........ ........ 0.781 0.011 1.35 382.658 ug/mL N/A N/A

SAM16 H5 1 0.905 ........ ........ ........ ........ N/A ........

SAM16 H6 2 0.709 ........ ........ ........ ........ N/A ........

SAM16_avg ........ ........ 0.807 0.098 12.14 416.579 ug/mL N/A N/A

SAM17 A7 1 0.582 ........ ........ ........ ........ N/A ........

SAM17 A8 2 0.858 ........ ........ ........ ........ N/A ........

SAM17_avg ........ ........ 0.720 0.138 19.17 314.804 ug/mL N/A N/A

SAM18 B7 1 0.717 ........ ........ ........ ........ N/A ........

SAM18 B8 2 0.804 ........ ........ ........ ........ N/A ........

SAM18_avg ........ ........ 0.761 0.044 5.72 358.951 ug/mL N/A N/A

SAM19 C7 1 0.718 ........ ........ ........ ........ N/A ........

SAM19 C8 2 0.918 ........ ........ ........ ........ N/A ........

SAM19_avg ........ ........ 0.818 0.100 12.22 431.076 ug/mL N/A N/A

SAM20 D7 1 0.589 ........ ........ ........ ........ N/A ........

SAM20 D8 2 0.684 ........ ........ ........ ........ N/A ........

SAM20_avg ........ ........ 0.637 0.048 7.46 234.176 ug/mL N/A N/A

SAM21 E7 1 1.341 ........ ........ ........ ........ N/A ........

SAM21 E8 2 0.476 ........ ........ ........ ........ N/A ........

SAM21_avg ........ ........ 0.909 0.433 47.61 567.132 ug/mL N/A N/A

SAM22 F7 1 0.378 ........ ........ ........ ........ N/A ........

SAM22 F8 2 0.270 ........ ........ ........ ........ N/A ........

SAM22_avg ........ ........ 0.324 0.054 16.67 29.700 ug/mL N/A N/A

STANDARDS

Name Well Replicate Abs. SD CV% Conc. Unit Cutoff R/I(%)

STD01 H1 1 0.320 ........ ........ ........ ........ N/A ........

STD01 H2 2 0.314 ........ ........ ........ ........ N/A ........

 3 (campuran).exp 09/21/18-21:12:14 6/7

STD01_avg ........ ........ 0.317 0.003 0.95 26.319 ug/mL N/A N/A

STD02 G1 1 0.495 ........ ........ ........ ........ N/A ........

STD02 G2 2 0.474 ........ ........ ........ ........ N/A ........

STD02_avg ........ ........ 0.485 0.011 2.17 119.691 ug/mL N/A N/A

STD03 F1 1 0.599 ........ ........ ........ ........ N/A ........

STD03 F2 2 0.705 ........ ........ ........ ........ N/A ........

STD03_avg ........ ........ 0.652 0.053 8.13 248.414 ug/mL N/A N/A

STD04 E1 1 0.895 ........ ........ ........ ........ N/A ........

STD04 E2 2 0.933 ........ ........ ........ ........ N/A ........

STD04_avg ........ ........ 0.914 0.019 2.08 577.479 ug/mL N/A N/A

STD05 D1 1 1.115 ........ ........ ........ ........ N/A ........

STD05 D2 2 0.710 ........ ........ ........ ........ N/A ........

STD05_avg ........ ........ 0.913 0.203 22.19 574.010 ug/mL N/A N/A

STD06 C1 1 1.183 ........ ........ ........ ........ N/A ........

STD06 C2 2 1.118 ........ ........ ........ ........ N/A ........

STD06_avg ........ ........ 1.151 0.032 2.82 1203.498 ug/mL N/A N/A

STD07 B1 1 1.184 ........ ........ ........ ........ N/A ........

STD07 B2 2 1.222 ........ ........ ........ ........ N/A ........

STD07_avg ........ ........ 1.203 0.019 1.58 1445.956 ug/mL N/A N/A

STD08 A1 1 1.303 ........ ........ ........ ........ N/A ........

STD08 A2 2 1.276 ........ ........ ........ ........ N/A ........

STD08_avg ........ ........ 1.290 0.013 1.05 2013.128 ug/mL N/A N/A

< End >

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