Materials and Methods

D-Limonene Bufotenine Extraction

1. Coarsely grind 50g of seeds into a powder, which contains the Bufotenine in a salt form; convert
this to its freebase form by adding 25g of Sodium Carbonate and just enough water to wet the
combination into a thick, pasty consistency. Stir this for 5-10 minutes, then dry completely.

2. Take the freebased seed powder and add it into the THP over the cotton ball filter. Pour 200ml
of Acetone over the seed powder, which will filter through the cotton and into a container of
your choosing. Take that very same Acetone and pour into the THP 10 more times(its quick so
might as well do it a bunch). Put the filtered Acetone into it’s own container. Take 200ml of
fresh Acetone, and repeat another 10 filters, adding that to the previously filtered Acetone.
Once more, take another 200ml of fresh Acetone, and filter 10 times, adding it to the mix. You
should have approximately 600ml of Acetone which has been run through the ground seed
powder many times.

3. Prepare your FASA by adding your 1g of fumaric acid to 150ml of acetone, and stir. Add the
FASA to the 600ml of filtered Acetone, for the Bufotenine to fumarate salt conversion, which
will settle to the bottom. Continue adding FASA until clouds no longer form in the mixture.
Pour out the Acetone/FASA mixture after all solids precipitate and keep the left-over solids.

4. Convert the left over solids back to Bufotenine freebase by adding a spoonful(2g) of Sodium
Carbonate and just enough water to turn them into a pasty consistency. Dry completely.

5. Pour 50ml of fresh Acetone into the Bufotenine freebase and stir for 5-10 minutes; when done,
pour the Acetone through a cotton filter into a glass dish to allow for it to evaporate over a
hotplate. Do this twice with a fresh 50ml of acetone. This should recover approximately 2g of
Bufotenine freebase.

6. Bring 35ml(more than you need but quite a bit of it evaporates during the boil) of D-limonene to
a boil (176 Celsius) and then add the Bufotenine freebase. The D-limonene will be hot enough
so that the Bufotenine will melt and become soluble in the hot limonene, but the toxins and
other undesirables will not be, and will remain. Let the mixture boil for 5 minutes.

7. Pour off the boiling D-limonene into a dish with a large surface area so that it can cool. When
pouring off the D-limonene you have to wait until it stops boiling, this only takes like 5 seconds
because otherwise the contaminates do not puddle up. After it stops bubbling immediately pour
it off because it is starting to drop the bufotenine. As soon as the D-limonene cools below it’s
boiling point, Bufotenine freebase will quickly fall out of it; emphasis on quickly! It happens in
mere minutes. Allow the D-limonene to continue to cool completely and then pour off the
remaining clear d-limonene. You should be left with approximately 1g of Bufotenine freebase,
allow residual d-limonene to evaporate off, using a slightly warm hotplate speeds this up
greatly.
 8. Repeat steps 6 and 7 again on the black goop that is left after the first d-limonene boil. This will
get out any left over bufotenine.

9. You can then repeat the d-limonene boil on the already purified bufotenine to purify it further.

10. Enjoy!

Materials Required
Source Material:

 50g Anadenanthera colubrina (seeds)

Solvents:

 100ml D-limonene

 800ml Acetone
Reagents/Desiccants:

 27g of Sodium carbonate

 1g (excessive but better safe than sorry) Fumaric acid

Equipment:

 Hotplate

 The Herbal Percolator

 Cotton Balls

 HDPE/Stainless/Glass Containers

 HDPE/Stainless/Glass Dishes