Manual de Servicio PDF

HumaCount 30 TS and 80TS
| Service Manual
Cat No. 16420/802

REVISION LIST OF THE MANUAL
Rev. /DATE. REVISION DESCRIPTION
01/2015-06 First edition
02/2015-12 Addition Cabling Diagram
03/2017-11 Special Service Settings & Removal/ reassembling of sample rotor
SYSTEM VERSION
HumaCount 80TS and HumaCount 30TS with software version 1.4 or higher
COPYRIGHT
Copyright 2017, Human Gesellschaft für Biochemica und Diagnostica mbH, Wiesbaden,
Germany. All rights reserved.
No part of this documentation may be reproduced in any form, nor processed, copied or
distributed by means of electronic systems, without prior permission of HUMAN in
writing. Since all precautionary measures were taken into account in producing these operating
instructions, the manufacturer accepts no responsibility for any errors or omissions. This
includes any liability for damage that could arise from possible incorrect operation based on this
information. Subject to changes without notice as result of technical development.
SERVICE UND SUPPORT

CONTENTS
TABLE OF CONTENTS
1 SAFETY INSTRUCTIONS 5
1.1 INTRODUCTION 5
1.2 USER WARRANTY 5
1.3 INTENDED USE OF THE INSTRUMENT 5
1.4 GENERAL SAFETY WARNINGS 6
1.5 DISPOSAL MANAGEMENT CONCEPT 6
1.6 BIOHAZARD WARNING 7
1.7 INSTRUMENT DISINFECTION 7
1.8 REPAIRS 7
2 SYSTEM DESCRIPTION 9
2.1 NAME AND SERIAL NUMBER 9
2.2 INTEGRATED SOFTWARE 9
3 INSTALLATION OF THE INSTRUMENT 11
4 REMOVAL OF THE INSTRUMENT 13
5 MAINTENANCE 15
5.1 SELF TEST 15
5.2 DEVICE STATISTICS 15
5.3 CLEANING AND GREASING DILUTOR BLOCK 15
5.4 CHECKING AND LUBRICATING DILUTOR PISTON TIPS 15
5.5 MICRO PISTON SEALING 15
5.6 CHECKING AND REPLACING WASHING HEAD 15
5.7 BLEACHING OF FLUIDIC SYSTEM 16
6 TROUBLE SHOOTING 17
6.1 EXPORT LOG FILE 17
6.2 SELF TEST 18
6.2.1 Selftest Screens 18
6.2.2 Actions in case of failed self test parameters 20
6.3 DEVICE INFORMATION 21
6.4 STATISTICS 21
6.5 LOG IN AS SERVICE 22
6.6 SERVICE MENU 25
6.6.1 Troubleshooting menu 26
6.6.2 Stress test 30
6.6.3 Needle position check 32
6.7 AMPLIFIER SETTING 32
6.7.1 Setting RBC and WBC amplifier gain 32
6.8 POSSIBLE CAUSES OF NOISE 32
6.8.1 Contaminated reagent 33
6.8.2 Bad earth grounding 33
6.8.3 External electrical noise 33
6.8.4 Internal noise sources 34
6.9 WARNING FLAGS 37
7 ASSEMBLED ANALYZER 39
7.1 FRONT PANEL 39
7.2 REAR PANEL 39
7.3 FRONT VIEW INSIDE 40
7.4 LEFT SIDE VIEW INSIDE HC30TS 40
7.5 RIGHT SIDE VIEW INSIDE HC30TS 41
7.6 LEFT SIDE VIEW INSIDE HC80TS 41
7.7 RIGHT SIDE VIEW INSIDE HC80TS 42
8 ELECTRONICS 43
8.1 COUNTING CHAMBER WITH ELECTRODES AND MEASURING TUBE 47
8.2 HGB MEASURING HEAD 48
8.3 CELL COUNTER AMPLIFIER BOARD 48
8.4 MAIN CPU BOARD 50
8.5 DIMMBOARD MODULE 52
8.6 OPTO SENSORS 53
8.7 REAGENT LOCK MODULE 53
8.8 VALVE ASSEMBLIES 54
8.9 FRONT PANEL ELECTRONICS 55
8.10 EXTERNAL POWER SUPPLY 56
9 MECHANIC AND FLUIDIC PARTS 57

9.1 SAMPLE PROBE MECHANICS 59
9.1.1 Vertical movement, setting timing belt tension 59
9.1.2 Vertical opto sensor and needle settings 60
9.1.3 Setting the horizontal movement 62
9.1.4 Setting the needle shaft 64
9.1.5 Removal/reassembling instructions of sample rotor mechanics 64
9.1.6 Setting the position of the chambers 80
CONTENTS
9.2 SAMPLE PROBE 80

9.3 WASHING HEAD 80
9.4 PUFFER RESERVOIR 81
9.4.1 HC30TS 82
9.4.2 HC80TS 83
9.5 MEASURING BLOCK 84
9.5.1 HC30TS 84
9.5.2 HC80TS 85
9.6 PUMP 85
10 OPERATION OF THE FLUIDIC SYSTEM 87

10.1 INITIALIZATION OF THE FLUIDIC SYSTEM 87
10.2 OPERATION OF THE FLUIDIC SYSTEM 88
10.2.1 HC30TS 88
10.2.2 HC80TS 95
11 FURTHER SERVICE OPTIONS AND FUNCTIONS 103

11.1 MAINTENANCE MENU 104
11.1.1 Cleaning 104
11.1.2 Calibration 105
11.1.3 Reagent status 108
11.2 SETTINGS 110
11.2.1 General 110
11.2.2 Measurement 111
11.3 SPECIAL SETTINGS SERVICE 112
11.3.1 Submenu “General” 112
11.3.2 Submenu “Maintenance Settings” 113
11.3.3 Submenu Hard Cleaning 114
11.3.4 Submenu “Control Measurement” 115
11.3.5 Submenu “Calibrator Measurement” 116
11.3.6 Submenu “Special Tubes” 117
11.3.7 Demo Mode 119
12 MISCELLANEOUS 121
12.1 MDA SCREEN 121
12.1.1 Event log 121
12.1.2 Command input 122
12.2 USB B CONNECTOR COMMUNICATION 122
12.3 RECOMMENDED KIT OF TOOLS 122
Safety Instructions 5
1 SAFETY INSTRUCTIONS
1.1 Introduction
This manual is considered part of the instrument and must be available to the
operator and the maintenance personnel. For accurate installation, use and
maintenance, please read the following instructions carefully.
In order to avoid damage to the instrument or personal injury, carefully read
the ”GENERAL SAFETY WARNINGS”, describing the appropriate operating pro-
cedures. Please contact your HUMAN authorised local Technical Service in the
event of instrument failure or other difficulties with the instrument.
1.2 User Warranty

HUMAN warrants that instruments sold by one of its authorised representa-
tives shall be free of any defect in material or workmanship, provided that this
warranty shall apply only to defects which become apparent within one year
from the date of delivery of the new instrument to the purchaser.
The HUMAN representative shall replace or repair any defective item within this
warranty period at no charge, except for transportation expenses to the point
of repair.
This warranty excludes the HUMAN representative from liability to replace any
item considered as expendable in the course of normal usage, e.g.: lamps,valves,
syringes, glassware, fuses, tubing etc.
The HUMAN representative shall be relieved of any liability under this warranty
if the product is not used in accordance with the manufacturer‘s instructions,
altered in any way not specified by HUMAN, not regularly maintained, used with
equipment not approved by HUMAN or used for purposes for which it was not
designed.
1.3 Intended Use of the Instrument

The instrument must be used for its intended purpose (see paragraph 2). It must
be operated in perfect technical conditions, by qualified personnel, in such [IVD]
working conditions and maintained as described in this manual, in the GENERAL
SAFETY WARNINGS. This manual contains instructions for qualified professional
operators.
6
1.4 General Safety Warnings

Use only chemical reagents and accessories specified and supplied by HUMAN
and/or mentioned in this manual. Place the product so that it has proper ven-
tilation.
The instrument should be installed on a flat, stationary working surface, that is
free of vibrations.
Do not operate in area with excessive dust.
Operate at temperature and at a humidity level in accordance with the specifi-
cations listed in this manual.
Do not operate this instrument with covers and panels removed.
Use only the power cord specified for this product, with the grounding
conductor of the power cord connected to earth ground.
Use only the fuse type and rating specified by the manufacturer for this instru-
ment.
The use of fuses with improper ratings may pose electrical and fire hazards.
To avoid fire or shock hazard, observe all ratings and markings on the instru-
ment.
Do not power the instrument in environments that are potentially explosive or
at risk of fire.
Prior to cleaning and/or performing maintenance on the instrument, switch off
the instrument and remove the power cord.
Only cleaning materials described in this manual may be used, as other mate-
rials may damage parts. It is recommended to always wear protective clothing
and eye protection while using this instrument.
All warning symbols that appear in this manual must be carefully observed.
1.5 Disposal Management Concept

The applicable local regulations governing disposal must be observed. It is the
user‘s responsibility to arrange for proper disposal of the individual components.
All parts which may contain potentially infectious materials must be disinfect-
ed by suitable, validated procedures (autoclaving, chemical treatment) prior to
disposal. Applicable local regulations for disposal must be carefully observed.
The instruments and electronic accessories (without batteries, power packs etc.)
must be disposed of according to the applicable local regulations for the dispos-
al of electronic components.
Batteries, power packs and similar power sources must be removed from elec-
tric/electronic parts and disposed of in accordance with applicable local regula-
tions.
HumaCount 30TS and 80TS | Service manual

Safety Instructions 7
1.6 Biohazard Warning

Analytical instruments for in vitro diagnostic application involve the handling of
human samples and controls which should be considered at least potentially in-
fectious. Therefore every part and accessory of the respective instrument which
may have come into contact with such samples must equally be considered as
potentially infectious.
The „BIOHAZARD“ warning label must be affixed to the instrument prior to first
use with biological material!
Figure 1
Biological Hazard Symbol
1.7 Instrument Disinfection

Before performing any servicing on the instrument it is very important to thor-
oughly disinfect all possibly contaminated parts. Before the instrument is re-
moved from the laboratory for disposal or servicing, it must be decontaminated.
Decontamination must be performed by authorised well-trained personnel, and
in observance of all necessary safety precautions.
1.8 Repairs
-- For all the operations described in this manual, wear latex

or nitrile gloves and wash hands thoroughly after comple-
tion of work.
-- Be careful with the sharp edges of the metal sheets after
removal of the covers.
8

System Description 9
2 SYSTEM DESCRIPTION
Since HumaCount 30TS and HumaCount 80TS have so much common character-
istics, we issue a common Service Manual covering both instruments. Informa-
tion herein applies for all instruments unless otherwise noted.
To be well up in the instruments, please read this manual carefully to have the
knowledge for servicing the instruments perfectly and avoid extra costs and
wasting precious time.
In this manual, we are using the following conventions:
HC30TS – stands for HumaCount 30TS

HC80TS – stands for HumaCount 80TS
This HumaCount 30TS / HumaCount 80TS Service Manual contains the functional
descriptions of all analyzers, operation of the fluidic systems, adjustments and
settings, and very important information for the service personnel about the
service operations and possible problems.
2.1 Name and serial number

Name: HumaCount 30TS / HumaCount 80TS Hematology Analyzer
Every instrument has its own serial number, which is printed
on the rear panel label and it can be read out from Device In-
Serial No.:
formation or from the self test submenu. This identity number
is write-protected.
2.2 Integrated software

The integrated software controls the instrument operations, displays, stores,
recalls data, and allows the user to perform QC and calibration procedures and
modify the user settings. The software version number can be read out from the
Device Information or from the Self test submenu.
Every HC30TS/HC80TS software version is upgradeable (using a USB flash drive)
by the latest program developed by HUMAN, and it can be downloaded from:
http://www.human.de
10

Installation of the instrument 11
3 INSTALLATION OF THE INSTRUMENT

For the installation of the instrument please refer to the relevant user manual.
12

Removal of the instrument 13
4 REMOVAL OF THE INSTRUMENT

To remove the instrument for e.g. service it must be shutdown according to the
instructions given in the user manual for turning the instrument off, preparing
for shipment. For transport make use of the original packaging material to pro-
tect the instrument from damage.
Disposal has to be done in accordance with local regulations.
14

MAINTENANCE 15
5 MAINTENANCE
The instruments should be checked and maintenance must be carried out every
6 months, or after 10 000 measurement cycles.
5.1 Self test

Run the built-in Self test and check the overall test result. Regarding failed self
test see section 6.2.2.
5.2 Device statistics

Check the device statistics to find common problems.
For details see section 6.4.
5.3 Cleaning and greasing dilutor block

The dilutor block driving wheels and gear bar should be cleaned from dirt and
must be greased between the gear bar and the support, and between cogged
wheels. Use the grease in the red/orange Eppendorf cup (Machine grease LX2)
for the cogged wheel connections and the white grease in the clear Eppendorf
cup (Photolube) for sliding bars. Both are part of the ‚Lubrication Kit‘.
5.4 Checking and lubricating dilutor piston tips

The cogged end of PTFE dilutor pistons should be cleaned and lubricated. Apply
just a thin layer, and move it along the perimeter of the piston, so that some of
the material goes into the gaps between the sealing rings. Use the clear silicon
grease in the blue Eppendorf cup which is part of the ‚Lubrication Kit‘.
5.5 Micro piston sealing

Check the condition of the micro piston sealing, and replace if necessary.
5.6 Checking and replacing Washing Head

Check the state of the washing head, and replace if necessary. After replacing
washing head, do not forget to perform correct adjustment of sampling needle
height (see section 9.1.2).
16
5.7 Bleaching of fluidic system

It is recommended to run a bleaching procedure to remove stains from the
fluidic system.
1. Connect 2-5%, hand warm, clean bleach solution to all reagent inputs.
2. Perform a stress test with 3 cycles.
3. Connect D.I. water to all inputs immediately after the 3 cycles are completed.
4. Immediately after connecting D.I. water perform a stress test with 5 cycles.
5. Connect the reagents to the reagent inputs again.
6. Perform a stress test with 5 cycles.

Trouble shooting 17
6 TROUBLE SHOOTING
6.1 Export Log file

When you are logged in as Service:
There will be two aditional buttons in the menu “Diagnostics”
1. Export Log: This Button will be helpful for Human Service Engineers for trou-
bleshooting the customers problems by connecting an USB stick you can ex-
port the analyzer log file with. Please with all claims you report to Human
send as well this file.
2. Clear Log: by pressing this button you will erase all stored data from the log
recording function.
Figure 2
Export log and Clear Log Buttons
18
6.2 Self test

The Self test can be used to check the operation of the instrument. It can be
selected by Maintenance, Diagnostics, Self test. By pressing the Start button the
Self test will be performed. Pressing Mode allows to chose between a full Self
test and Fast Self test.
Fast Self test checks a lower number of functions of the instrument. The result of
the selftest can be printed by pressing Print. The result of the Self test can saved
to USB flash disk by pressing Save. A USB flash memory must be connected to
the instrument. Human Tech-Service may ask for this file in case of any issue.
Figure 3
6.2.1 SELFTEST SCREENS

Every measured value has a check mark if it is in the acceptable range, or an X
and a minus or plus sign if it is below or above the normal range.
Figure 4

Trouble shooting 19
Overall result indicates Success only if all individual results are within accept-
able range. Otherwise it will show Failed.
HGB measured impulses per second
Vacuum reports pump operation (vacuum made by the pump in a 10-second
period of time).
Drift represents pressure loss of vacuum measured in a 10-second period of
time.
Measuring Electrode voltage, current and offset.
Amplifier Noise test during a 5-second period.
Amplifier test by generating 20000 test pulses, incl. gain related Peak value,
noise related Deviance.
Fast blank measure, the device performs a fast blank measurement. This num-
ber is the PLT count. Fast blank probe min and Fast blank probe max voltage
are relative numbers during fast blank measurement. For HC30TS this is single
measurement while for HC80TS separate results are shown for both measure-
ment chambers and amplifier channels.
20
6.2.2 ACTIONS IN CASE OF FAILED SELF TEST PARAMETERS

Following recommended actions are given in case of failed parameters of the
self test:
Table 1
Parameter Mark Possible reason Remedy
HGB Low HGB head not connected or Check HGB LED during meas-
HGB LED or sensor out of urement. Check light pathway
order. Pptical pathway through chamber.
disturbed. Check HGB head electrical
connection. Replace if necessary.
High Stray light or HGB LED too
Close door or check optical path-
bright or HGB head damaged.
way. Replace if necessary.
Vacuum High Pump or sensor failure or Check function of pump and

leakage. sensor. Check for leakage.
Drift High Leakage in pneumatics. Check tubing in pneumatics.
Electrode voltage Low or High Fault on Main or Amplifier
Change Main or Amplifier board.
board.
Electrode offset Low or High Fault on Main or Amplifier
Change Main or Amplifier board.
board.
Electrode current Low or High Fault on Amplifier board. Check measuring circuit.
Measuring tube clogged?
Noise test High Instrument not grounded. Check mains ground lead
Amplifier test Low Amplifier Boards is not con- Check cables and connectors
nected to Main board. coming from the Amplifier
High Instrument not grounded. Check mains ground lead
Peak Low or High Fault on Amplifier board. Change Main or Amplifier
board
Deviance High Instrument not grounded. Check mains ground lead
Fast blank measure High Contaminated system. Run cleaning cycle
Fast blank probe High or Low Fault on MAIN or Amplifier Check measuring circuit for in-
min board or measuringcircuit terruptions. Check for correct liq-
disturbed uid in chamber (diluent). Change
depending on observation:
chamber, measuring tube, ref-
Fast blank probe High or Low erence elctrode, cables, amplifier
max or Main board.

Trouble shooting 21
6.3 Device information

Under Maintenance-Diagnostics you will also find the function Device informa-
tion. This function displays in a short form the serial number of the instrument
and the versions of the different software.
Figure 5
6.4 Statistics
In this screen the statistics of different actions and events is shown. This helps
to identify issues which need attendance. Once checked for service issues press
Clear to reset the count to zero.
Figure 6
22
6.5 Log in as SERVICE

To log in as Service procede as follows:
1. In Main menu press the Exit button.
Figure 7
2. Press Logout (USERNAME).
Figure 8
3. Press Login
Figure 9

Trouble shooting 23
4. At Log In screen, touch the screen at the 4 positions as indicated in the pic-
ture below, so the Login name will change to Service.
Figure 10
1 4
2 3
5. Touch the Password field and type in the Service password which is 6484 and
press Enter to confirm
24
Figure 11
6. Press Accept button in Log in screen.
Figure 12
The display will show the Main menu again. Several Service options are now
available in different parts of the software.

Trouble shooting 25
6.6 Service Menu

Touch Maintenance-Diagnostics-Service to enter the Service menu.
The Service menu is intended for servicing and operation checking purposes.
Figure 13
26
6.6.1 TROUBLESHOOTING MENU

The Troubleshooting menu provides tools to test different components of the
instrument.
Figure 14
6.6.1.1 Motors
From the Motors submenu each or all motor movements can be checked.
Pressing Needle will move the sampling needle up and down.
Pressing Horizontal will initiate the horizontal movement of the needle in com-
bination with the rotation of the sample door.
Pressing Dilutor will move the dilutor(s) up and down.
Pressing Test all will initiate a combined movement of all mechanically moving
parts.

Trouble shooting 27
Figure 15
By testing the movements the optos are also checked since the end points of
a movement in one direction is dependent on the corresponding opto switch.
However a separate testing of the optos might be necessary.
While the motor test is running the progress will be displayed on the screen. Any
test must be stopped by pressing Abort.
Figure 16
28
6.6.1.2 Optos
Optosensor states can be checked in this screen.
The mechanical parts must be moved manually for this test. By moving the
flags/holes in and out of the optos the state of the correspondig opto switch
can be observed on the screen by the change of the colour of the square box
between black and white.
-- Black colour indicates that the opto switch is interrupted (no light passes
through).
-- White colour indicates that the opto switch is free (light passes through).
Figure 17
6.6.1.3 Valves
This menu allows to check the correct function of the valves and the pumps.
Pressing the buttons of the valves switches them. 1 stands for electronically
forced state. By pressing a Pump button the corresponding peristaltic pump is
switched on/off. The current vacuum in the puffer reservoir is displayed under
Pressure.

Trouble shooting 29
Figure 18
6.6.1.4 Amp. Offset

You can check the offset on the amplifier board. The current offset and the ac-
ceptable range are displayed.
30
Figure 19
6.6.2 STRESS TEST

During Stress test the instrument performs measuring cycles without sample
(blank measurements) continuously. This can be used for burn-in tests or to
check the pneumatic system after changing any main fluidic part.
You can have information about stability, cleanliness, HGB operation, and
counting time stability. Results are displayed in table format.
You can detect any kind of noise, or bubbles in the system if the PLT is not stable
low.
To start stress cycles press Start or Start 10. Start will start stress cycles until the
process is stoppedby pressing Abort. Start 10 will perform 10 stress cycles.
Figure 20
...
While Stress test is running the progress will be displayed.

Trouble shooting 31
Figure 21
...
Once Stress test is finished the result can be printed by pressing Print or stored
to a USB flash memory be pressing Save.
Figure 22
32
6.6.3 NEEDLE POSITION CHECK

With Needle position check the correct height of the needle can be set. If the
needle opto is set correctly, the lower end of the needle is co-planar with the
bottom plane of the washing head. If it does not, adjust the needle opto up or
down and check the position again (see section 9.1.2).
Pressing Needle will move the needle into a defined position.
6.7 Amplifier setting

To modify the gain of the pre-amplifier(s) select Settings-Measurement-Service.
6.7.1 SETTING RBC AND WBC AMPLIFIER GAIN

If the correct MCV value cannot be obtained by calibration the amplifier gain
of RBC measurement can be increased or decreased by approx. ±10%. Please be
advised that changing this value requires to recalibrate the device to get proper
results!
If the WBC diagram is shifted too far to the left or right in WBC histogram, the
amplifier gain of WBC measurement can be increased or decreased by approx.
±10%. Please be advised that changing this value requires to recalibrate the de-
vice to get proper results! Confirm the changes by pressing Accept.
Figure 23
6.8 Possible causes of noise

Generally high count of any particle - even if you think it should be low, or near
zero - can be caused by noise, i.e. something interfere with the measurement.
The most important thing in these cases is to identify the source of the noise,
otherwise you cannot protect the system against it. Noise can come from sever-
al sources, and the different sources add up. However only one of them may be
enough to cause problems.

Trouble shooting 33
6.8.1 CONTAMINATED REAGENT

The most probable cause are particles in the reagent, and therefore the PLT
blank is continuously high (e.g. always 30-40). You can easily sort out this case
by replacing diluent with a new container. PLT blank must go down during
several blank measurements (below 10).
How can a good reagent become bad with time?

-- If the reagent tube was contaminated, and some bacteria begin to grow
inside, once you put an infected reagent tube into a new tank, by time it
can become infected as well, i.e. the background (PLT blank) becomes high.
Wash the reagent tube - which is in connection with the reagent - with 1% of
bleach solution, then rinse with clean distilled water or diluent. It can avoid
the bacteria to grow inside.
-- If container is open – and cap is not installed or closed - external dust can fall
into the reagent.
6.8.2 BAD EARTH GROUNDING

In this case external - ground referenced - noise can get into the system by
ground coupling. If system ground is not good enough, ground terminal can be-
come a noise source as well, i.e. external signals will be coupled into the system
instead of protecting it. If no earth ground is available, you can use a screw at
the rear panel to connect a ground potential to the case, so that noise immu-
nity can be increased. Measure voltage on ground terminal to make sure earth
grounding is correct. AC voltage lower than 1V is accepted in this case.
At some places - as a bad practice - electricians like to connect earth ground

terminal to neutral wire. Depending on the resistance of the neutral back
wire (where it is really earthed), several volts can appear, and this way any
inductive noise will be coupled into the instrument. It is better to create a real
earth grounding and connect it to the rear screw.
6.8.3 EXTERNAL ELECTRICAL NOISE

If another instrument is near the analyzer can radiate electromagnetic sig-
nals in the 1 kHz - 100 kHz frequency region it can be picked up by the system
(especially if they are very close to each other, or the grounding is not quite per-
fect).
34
You can easily identify this noise source: by relocating the instrument
noise (high PLT blank) disappears. In this case you have to identify the possible
noise source (switch mode power supplies, computer monitors, since they are
not shielded, centrifuges due to high switching noise of rotor contacts, etc.), the
power of the electromagnetic source, because if high power is present, maybe
relocation does not solve your problems, sometimes the electric power supply
makes the coupling, so UPS solves the problem.
Another source of coupling in external noise can be the reagent tanks and tubes.
Especially radio transmitters can cause problems of radiating so that even the
reagents (diluent) guides in the noise. A metal pack for the diluent tank, then
a good earth grounding of this metal box allows this coupling to disappear
forever.
6.8.4 INTERNAL NOISE SOURCES

The most annoying but real cause is some sort of internal noise. The reason for
this phenomenon is that inside electrode - hot point - of the measuring circuit
must be well insulated from surrounding electronics, otherwise inside noise
sources can take their effect.
6.8.4.1 Bad chamber insulation

-- Bad shielding of the chamber (floating shield couples signals to the
chamber, and does not prevent against them). Check grounding of shield,
remove it and clean the surface between the shield and the metal base.
-- Bad reference electrode connection (floating ground reference). Repair is
required.
-- Bad sealing of aperture. Replacement of measuring tube is required.
-- Broken measuring chamber starts to conduct through the gaps (ground
path). Replacement of chamber is required.
-- Contaminated draining tube starts to conduct due to protein or lipid build-
up. It is very easy to identify this case. After replacing the drain tube of the
measuring chamber (mainly WBC), WBC histogram peak, or PLT becomes
low soon. Normally a good cleaner is required to dissolve lipid or protein
build-up. Sometimes the cleaner is not strong enough to keep this tube clean
enough. Periodic washing using 1% hand warm bleach solution helps.

Trouble shooting 35
6.8.4.2 Bad insulation of electronic signal paths:

In these cases check for any capacitive coupling of electronic signals to the
chamber:
-- Interference with HGB head (high-frequency signal is coupled to the cham-

ber). HGB head metal parts must be grounded. The ground comes externally,
it must be in place, otherwise HGB head does not shield, but couples in noise.
-- Interference with internal high voltage inverter (high-frequency signal is
coupled to the chamber). Repair is required: avoid near contact of HVB cable
to chamber or shielded amplifier cable.
-- Interference with internal start button (polling signal to start button may
cause noise). Guide start button wires as far from chamber as possible. You
may try mix them up on the start micro-switch if applicable.
-- Interference with display cable (high-frequency LCD signal is coupled to the
chamber by the ribbon cable). Keep the ribbon cable far from the chamber.
-- Interference with CPU fan or other digital logic traces (CPU fan or other
digital signal radiates to chamber or to the shielded amplifier cable). Try
keeping the ribbon cables far from the chamber and shielded cable.
6.8.4.3 Bad components or connections:

-- Bad soldering, salt residuals or component failure on amplifier (especially
if some reagent could get in the amplifier section). Cleaning of PCB/elec-
trode socket or replacement of amplifier is required. Check for the correct
soldering of reference cable and its connector.
-- Circuit board bad soldering or component failure. Check the shielded cable
connections as well. Sometimes inside out connection (hot electrode goes
outside as a shield) is the problem: both ends of amplifier signal cable must
be reversed.
-- Analog signal ribbon cable (it picks up noise). Check the ribbon cable
between the circuit board and the amplifier. Maybe it is pinched under some
screws or components. This may cause trouble and even noise.
36
6.8.4.4 Pneumatic failures, liquid paths that conduct noise into the chamber:
-- Liquid remains under the chamber in drain tube (during measurement the
conducting liquid remains inside the drain tube making noise to appear
there).
• Check chamber draining path for clogging or salt crystals.

• Check the pump operation. Since draining of the chamber goes under
pressure control, maybe a bad pressure sensor or connection can cause
trouble.
• Clean the draining path. Do not use alcohol, but bleach. Replace chamber
if necessary.
-- Liquid remains in the washing inlet at top of the chamber (during measure-
ment the conducting liquid remains inside the chamber wash tube making
noise to appear). The software is not compatible with the mechanics, or re-
lated valve is bad/partly clogged, or the tubing is clogged/loose.
-- Lyse path guides in noise (during counting, if the a liquid in the draining tube
is touching lyse reagent in T-fitting, noise can appear). Check the lyse path,
and the lyse valve as well.

Trouble shooting 37
6.9 Warning flags

Analyzer SW displays warning flags for each individual measurement to noti-
fy user about status of results. The following table summarizes warning flags
and gives explanation of their possible cause and a few hints to overcome the
problem.
Uppercase letters refer to WBC or HGB problems.
Flag Meaning Recommended user action
No WBC 3-part Possible lyse problem. May occur in pathological

E
differential lymphocytosis.
Repeat the blank measurement. If HGB blank is

HGB blank is high, not stable there are probably bubbles in the WBC
H
or no HGB blank chamber: Run a cleaning and try blank again.
Close the side door if open during measurement.
Repeat the blank measurement, or run prime lyse
WBC blank is high,
B and try blank again. Possible lyse contamination,
or no WBC blank
or noise problem.
The analyzer found that the cell count is higher
linearity range
than the linearity range of the analyzer. Make a
M exceeded in WBC
pre-dilution, and run the same sample in predilut-
stage
ed mode.
RBC cells were detected during the WBC meas-
RBC cells found in
urement. Either the lyse reagent is not effective
R sample during WBC
enough (volume should be increased) or the RBC’s
stage
in the sample are somewhat lyse resistive.
Probably large PLTs or clumped PLTs are present

in the sample. Usually caused by the nature of the
sample. cat and goat samples tend to clump. In-
tensive, but careful mixing of the sample (e.g. Vor-
W WBC 3-part warning
tex) can help remove the clumps. If the rerun sam-
ple gives the same results, consider that WBC and
NEU values seem higher because of the clumps.
Lyse modification can’t solve the problem.
38
Typically insufficiently lysed RBC’s interfere with

the start of the WBC histogram. Repeating the
measurement with an increased lyse volume
RBC-WBC limit
L should provide better separation. If the repeated
warning
run reports very similar results then the MON and
NEU results are VALID but the WBC and LYM re-
sults may be higher because of interfering RBCs.
Aperture clogging. Perform cleaning and repeat
the measurement. If it is a general problem, please
contact your Service Personnel. Low temperature
C WBC clogging
reagents can cause it as well (mainly diluent), in
this case you will have to wait until they reach
room temperature.
Warning flags in lowercase refer to RBC or PLT problems.
Flag Meaning Recommended user action

The analyzer found that the cell count is higher
Linearity range
than the linearity range of the analyzer. Make a
m exceeded in PLT/RBC
predilution, and run the same sample in predilut-
stage
ed mode.
Multiple or incorrect RBC peak(s) detected. Try to
k RBC peak error
run the sample again.
PLT and RBC cells could not be separated, or the
PLT / RBC limit not
l histogram remained high in the PLT/RBC valley
correct
range.
c RBC/PLT clogging The same action as in case of the C warning flag.
Run cleaning and repeat the blank measurement
PLT blank is high, Diluent or system cleanliness problem. If it is
p
or no PLT blank stable high, replace the diluent by opening a new
tank.
RBC blank is high or
b Same action as in case of warning flag p.
no RBC blank

Assembled Analyzer 39
7 ASSEMBLED ANALYZER
7.1 Front Panel
Figure 24
HCxxTS front panel
1 LCD
2 LED
3 Sample door
1
7.2 Rear Panel
Figure 25
2
HCxxTS rear panel
1 Reagent and waste connec-

3 tors
2 Power switch
3 USB A connectors
4
4 USB B connector
5
5 Ethernet connector
6
6 Grounding connector
7
7 Reagent key connector
8
8 Power connector 12V DC
1
40
7.3 Front view inside
Figure 26
Font view inside
4
1 Main board
2 Waste pump
5
3 Left side USB connector 1
4 Vacuum buffer
5 Dilutor motor
6 Sample probe vertical motor 6
7 Sample probe and door hori-

zontal movement mechanics
8 Sample probe and door 7
2
movement motor
3
8
7.4 Left Side view inside HC30TS
Figure 27
HC30TS electronics side
1 Main board 1
2 DIMMBOARD

Assembled Analyzer 41
7.5 Right side view inside HC30TS

5
Figure 28
6 HC30TS pneumatics side
7 1 Micro/Sample syringe
1
2 Sampling needle with wash-
8
ing head
3 Measuring chamber
2
4 Valves 6-10
5 Valves 1-5
3
6 Vacuum buffer
7 Macro/Diluent syringe
8 Lyse syringe
7.6 Left side view inside HC80TS
Figure 29
HC80TS electronics side
1 1 Main board
2 DIMMBOARD
2 3 2 amplifier boards
3
42
7.7 Right side view inside HC80TS
1
Figure 30
HC80TS pneumatics side
7
1 Valves 1-5
2 Micro/Sample syringe 2 8
3 Sampling needle with wash-
ing head 9
3
4 RBC/PLT measuring chamber 10
5 WBC/HGB measuring cham-
4
11
ber 5
6 Valves 6-11
7 Vacuum buffer
8 Lyse syringe
9 3 diluent syringes
6
10 Dilutor 1
11 Dilutor 2

Electronics 43
8 ELECTRONICS
HC30TS/HC80TS contain the following electronic parts:
1. Counting chamber (2 pcs in HC80TS) with electrodes and measuring aper-

ture
2. HGB Measuring Head
3. Cell Counter Amplifier Board
4. MAIN CPU Board with Dimm-PC and measurement processing unit, 4 mo-
tor controllers, valve & pneumatic controller/driver, pump driver and power
supply for internal printer (+7.5V) and digital circuitry (+5V, +3.3V)
5. DIMM-PC module
6. Motors with opto-boards of needle moving motor (H) and sample rotor/nee-
dle moving motor (V)
7. Dilutor block with opto-board for sampling, diluent, lyse and cleaner
8. Valve boards
9. Peristaltic Pump (2 pcs in HC80TS)
10. USB interface
11. Graphic LCD Display Module with touch-screen
12. Start Button Panel
13. Internal Printer
44
Figure 31
diagram
HCxxTS electronics block

MAIN Board (CPU, Pneumatic and Power Board) Fluidic System
Amplifier Board
Internal printer
interface
Motor driver 1 Rotor motor Counting chamber
(MIX/WBC in Cell counter Amplifier
HC60TS)
Motor driver 2 Motor driver 1
12 VDC
external Power Dilutor motor( 2 in HGB measuring head
Supply Motor driver 3 HGB interface
HC60TS)
Motor driver 4 (used

in HC60TS only) Snap in optosensors
RBC chamber
Digital power 5V (in HC60TS only)
Opto sensor interface
Valve block I _II
External printer
Digital power 3.3V Valve drivers
Pump
Pump driver
USB A 1
FPGA Pressure sensor
XCS30XL
USB A 2
External keyboard Measure control
DIGIO Display
USB A 3 Assembly
HVB(50V, 150V)
USB A 4
USB stick
DC/DC ±12V
320*240 (QVGA)
USB DIMMPC TFT modul
HUB AMD Elan SC-520 Start
PIC24 w/ touchscreen
μController To PIC ADC button & w/ LED backlight
Status
LED board
USB B
LCD backlight driver
Sign Collection Board
External PC
BLTS v1.0
Touchscreen
interface
Start Button, Status

LEDs interface
LCD driver
Valve board Valve board P1 Vertical optosensor
Built-in Printer
1-5 6-10
1-10 10-20 Sample rotor/Horizontal
movement optosensors
Valves cable 16420/176 Left Right

13-15
Dilu Vertical Horizontal

Motor (3) Motor (2) Motor (1)
Electronics
M M M Printer cable 16420/179 1-3 4-6
Opto cable 16420/175

…... …... …... …...
USB cable USB6

USB4
Display USB5 Dilutor optosensors
USB cable
LCD 16420/531 Upper Lower
Touch Control
Board
16420/533 Front USB
Internal USB cable panel 7-9 10-12
USB Display
Other side of
Panel Power switch
the board Internal USB cable 16420/619
17
Amplif cable
16350/176
Power input
4
ACS Main board …...
LCD Backlight 16420/528
Panel
16420/532
2 LED control cable
LED USB panel Reag prot

16430/218 USB cable 4 pin panel
LED cable
16430/169
Display power cable
Digio cable 16420/328
Amplifier
board
16420/321
Size Cat. no. Drawing name Version
Drown by: Eszter Tóth A3 New Humacount30TS cabling diagram V1.0
Released: 13.10.2015 Scale N/A CO#: Page 1/1
Figure 32
HC30TS cabling diagram
45
46
Figure 33
HC80TS cabling diagram

Valve board Valve board P2 P1 Vertical optosensor
Built-in Printer
1-5 6-11
1-10 10-20 Sample rotor/Horizontal
movement optosensors
Valves cable 16420/176 Left Right

13-15
Dilu motor Dilu motor Vertical Horizontal

left (4) right (3) Motor (2) Motor (1)
M M M M Printer cable 16420/179 1-3 4-6
Opto cable 16420/175

…... …... …... …...
Dilu opto cable 16420/623
USB cable USB6

USB cable USB4
Display Dilutor optosensors
USB5
16420/531 Upper left Lower left
Touch Control
Board
16420/533 Front USB
Internal USB cable panel 4-6 7-9
USB Display
Panel Other side of
the board
Upper right Lower right

17
Amplif cable
16420/517
4
Power input 7-9 10-12
LCD Backlight
Panel ACS Main board …...
16420/532 16420/528
2 LED control cable
LED
LED cable
Display power cable USB panel Reag prot
16430/218 USB cable 4 pin panel
Amplif cable
16430/169
16420/172
Digio cable
EIB cable RBC
Amplifier Amplifier
board board
EIB cable 16420/516 16420/516
WBC
Measuring block Size Cat. no. Drawing name Version
Drown by: Eszter Tóth A3 Humacount80TS cabling diagram V1.0
Released: 14.10.2015 Scale N/A CO#: 1759 Page 1/1
Electronics 47
8.1 Counting chamber with electrodes and measuring tube

Impedance method is used for determination of volume and number of cells.
In this method a known volume of dilution is drawn through a small aperture.
Constant current is passed through the aperture from one side to the other.
When a cell passes through the aperture, it causes a change in resistance, which
generates a voltage pulse.
The amplitude of the voltage pulse is proportional to the ratio of cell volume
per aperture volume. This is used to determine the volume of cells. The num-
ber of cells can be obtained by counting the pulses. In the HC30TS there is one
cell-counter probe: the aperture size is 70 µm and has a reference electrode
assembly and U-shaped metal fixing as it is shown in the figure below.
The aperture is made of ruby and it is molded into the end of the measuring tube.
In the HC80TS there are two separate chambers: one for counting RBC with an
aperture of 70 µm, and another for MIX/WBC/HGB with 100 µm aperture.
Figure 34
Measuring chamber
1 Chamber
2 Electrode
3 Draining connection
8 4 Opening for measuring tube

1
5 Measuring tube
6 U-shaped metal fixing
7 Reference electrode
7 8 Washing inlet
3 4 5 6
48
8.2 HGB Measuring Head

Hemoglobin head is placed around one measuring chamber in all instruments.
It contains: light source (LED) at 540 nm wavelength and Photo Detector
(TSL235). The Photo Detector converts the light to frequency. The HGB concen-
tration is a logarithmic function of this frequency measured by the FPGA circuit
on the MAIN board.
Figure 35 3
HGB measuring head
2
1
1 LED
2 Connection to amplifier
3 TSL 235
The frequency output signal of TSL235 is counted by a digital counter in the

FPGA circuit.
This counter counts up while the LED is on and counts down while the LED is
off. The LED and direction of counting are switched with a 100 Hz signal. This
method provides “real time backlight correction”, which makes the HGB meas-
urement more precise in changing backlight environment situation as well.
There are two kinds of HGB measurement:
-- Sample measurement (before RBC counting)
-- Diluent/blank measurement (in WBC washing phase)
! Due to enhanced HGB tech-

nology, HC30TS/HC80TS
less sensitive to incident light
is
The HGB result is calculated from these measurements by:
HGB ≅ log (CNTdiluent light / CNTsample light)
changes. However, it is recom-

mended to keep side door closed 8.3 Cell Counter Amplifier Board
during measurements. The amplifier board includes its own voltage regulator, connection interfaces to
HGB head and to MAIN board. There is a current generator circuit on it, which
works from 50V measuring voltage (generated by MAIN) and the probe voltage
(DC) is amplified with a voltage follower (output: ELV). Nominal measuring cur-
rent is 870 µA.
While HC30TS has 1 amplifier board, HC80TS is equipped with 2 amplifier boards.
The board used in HC30TS differs from those used in HC80TS, even though the
difference can not bee seen. As a consequence the cat. nos. are different.

Electronics 49
Figure 36
Amplifier board (front)
1 Connection to DIGIO on
3
Main board
1 2 Connection to CSA1 on Main

board
3 Connection to HVB on Main
board
Figure 37
Amplifier board (back)
1 Connection to electrode
2 Connection to HGB head
The amplifier board includes one input connector for the chamber (measuring
electrode). There are two opto switches (U1, U3) to connect high voltage to the
probe with HSW signal and isolate the input of the amplifier. Test circuit makes
possible to generate test pulses (with TEST and PLS signals through FETs) for
checking the proper operation of the amplifier channel.
50
Amplifier board includes a 3-stage main amplifier channel, which gains input
signal to the 0...3.3 V range (this is the input range of the A/D converter, which
is placed on the MAIN board). The RSW signal changes the gain (RBC, WBC) in
the feedback of the second amplifier stage with U2 (MAX319) analog switch.
Amplifier gain and offset are adjusted by software.
DHON signal switches on the LED and the MVON signal – which is active during
counting – switches off the Photo Detector in the HGB head, to prevent noise
generated by the HGB detector.
The other side of the amplifier board contains special connectors for the cham-
ber and the HGB head (JP4).
8.4 MAIN CPU Board

This board contains all electronic componentes to control the instrument. It has
a central connection to the DIMMBOARD which is the computing unit of the
instrument.

Electronics 51
Figure 38
Main board (front)
1 DIMMBOARD
1
52
Figure 39 21 20 19 18 17
Main board (back)
1 Horizontal motor (sample)

2 Vertical motor (sample)
3 Dilutor motor 16
1
4 Second dilutor motor
2
(HC80TS) 15
3
5 not used ???
4 14
6 LED front panel
13
7 Reagent protection board
5 12
8 Measure cable amplif. (2nd
connector HC80TS only) 6
9 DIGIO cable to amplifier 7
10 Power for front panel
11 Power switch
12 Touchscreen USB
11
13 Left side USB connector
14 LCD USB
8
15 Second dilutor opto sensors
(HC80TS only)
16 Opto sensors 9
17 Internal printer 10
18 Waste pump
19 Waste pump (2nd pump
HC80TS only)
20 Valves
21 Pressure sensor
8.5 DIMMBOARD module

The DIMMBOARD is the central computing device in the instrument. It keeps the
software to run the instrument and the acquired data.

Electronics 53
Figure 40
DIMMBOARD
8.6 Opto sensors

Opto sensor snap-in modules are responsible for checking motor positions.
There are 6 opto sensors in HC80TS, and 5 in HC30TS (see cabling diagram).
Figure 41
Opto sensor
8.7 Reagent lock module

The reagent lock module ensures, that only reagents provided by Human can be
used on the instruments.
Figure 42
54
8.8 Valve assemblies

There are two kinds of valve assemblies: Valve board 1-5 and Valve board 6-10
or 6-11.
HC30TS has 10 valves, while HC80TS has 11 valves. The valve boards are con-
nected to controller and driver chips which are located on the MAIN board.
Figure 43
Valve assembly

Electronics 55
8.9 Front panel electronics
Figure 44
Front panel assembly
1
1 Flat cable to touch panel
2 Touch panel controller board
3 Cable for LCD contrast
2 11 4 Flat cable to LCD

5 LCD power cable
6 LCD backlight connector
3 7 LED board
8 LCD driver board
10 9 LCD USB communication
4
cable
10 LCD controller board
11 Touch panel USB communi-
cation cable
5
9
7
56
8.10 External power supply

The analyzer works with an external power supply. The figure below shows the
power supply unit generating 12VDC.
The power supply modules have an auto range input, which makes possible
to use them with 230V or 115V mains outlet and it has the CE and UL safety
certificate. The input socket of the power supply is a standard 3-terminal plug,
with power cable connection; the output is a coaxial power connector.

Mechanic And Fluidic PARTS 57
9 MECHANIC AND FLUIDIC PARTS

HC30TS and HC80TS Hematology Analyzers consist of the following mechanic
and fluidic parts:
1. Sample rotor
2. Sampling needle
3. Washing head
4. Sample/Horizontal moving unit
5. Micro Dilutor
6. Dilutor
7. Chamber
8. Cell-counter probe
9. Puffer reservoir
10. Pump
11. Valves
12. Tubing
58
Figure 45
Fluidic scheme HC30TS V2 DilWash
1
V4 DilNeedle
3
1
2
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure
V1 Cleaner
Meter 3
P V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
3
Micro Dilutor Lyse Dilutor V8 Bubble
2
M3
Macro Dilutor HGB
2
V7 DrainAperture
M2
1
LYSE Ver
2
V6 DrainChamber
2 1
1
3
V10 DrainPuffer Sample/

M1 H motor
Pump
2 1 1 2 3-way
Stepper Motor 2 -way Valve
M1 Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
Figure 46 P
Fluidic scheme HC80TS V1 DilWash

2
3
Buffer
1 reservoir
V4 DilChamber 3
2
V2 DilNeedle
2 1
3
DILUENT
V3 Cleaner
1 2 V5 LyseWBC
3
2 1
1 2
V10 3
3
1 2
V7 Aperture
CLEANER
Sampling
probe 1 2
1
Vertical MIX
RBC
SAMPLING
M2 WBC V9 Bubble 3
motor 2
DILU
DILU
LYSE
70µm 100µm
Aperture Aperture
HGB
head
2
Sample M1 V6 Drain
Rotor Chamber 2
1 V8 Drain
Chamber
1
2
V11 DrainPuffer 3
1
M3 M4
Dilutor Dilutor LYSE
motor motor
Pump 1
2 1
M1 Stepper Motor Pump 2
2 1
1 2 2-way Valve
Closed = Off WASTE
Open = On
3 Size Cat number Drawing number Version

3-way Valve
1 2 1-3 = Off A4 HC80TS fluidic schematics V1.0
2-3 = On Drawn by Eszter Tóth
Release 17.09.2015 Scale No scale CO# Page 1/1

9.1 Sample probe mechanics

The sample rotor unit uses a stepper motor. The rotor has opto sensors for po-
sitioning. A second stepper motor is used for vertical probe movement. An opto
sensor at the upper end is used to determine the home position.
Figure 47
Sample probe and door
mechanics
9.1.1 VERTICAL MOVEMENT, SETTING TIMING BELT TENSION

The timing belt tension could be set with positioning the vertical motor using
the oval holes in the mounting plate.
1. Untighten the screws which fix the vertical motor
2. Push the motor down. Tighten the screws.
60
Figure 48
Setting the belt tension
3. Verify the tension. The vertical rod side of the belt must be pushed towards
the rod. The distance between the rod and the belt must be 2-3mm.
Figure 49
Verifying the belt tension
9.1.2 VERTICAL OPTO SENSOR AND NEEDLE SETTINGS

The vertical opto sensor should be set as follows:
The flag (2) mounted on the vertical needle moving mechanism must run freely
between the two parts (1) of the opto-sensor.

Figure 50
Home opto sensor and flag for
vertical needle movement
1 Opto sensor
2 Flag
The vertical position can be set by loosening the two mounting screws of the
opto sensor and moving it up or down. In the correct setting, the lower end of
the needle is coplanar of the lower plane of needle washing head. The vertical
position of the needle is checked in the Service menu (see section 6.5.3 on page
61). If the the end of the needle is not coplanar with the lower plane of the
washing head adjust the opto sensor accordingly.
Figure 51
Setting opto sensor for vertical
needle movement
62
9.1.3 SETTING THE HORIZONTAL MOVEMENT

Check the correct position of the needle bracket according to the description
below. If the bracket is not close to the cantilever do the following:
1. Release the set screw which fixes the cogwheel segment to the vertical shaft
2. Push the needle bracket forward while the cogwheel segment stays in the
same position. It might be necessary to push the bracket backwards first
with the cogwheel segment moving turning synchronously.
3. Start the horizontal movement from Maintenance-Diagnostics-Service-Trou-
bleshooting-Motor test by pressing Horizontal.
4. The needle bracket will be moved forward. The movement of the bracket will
be stopped by the cantilever, while the cogwheel segment still moves.
5. Press Abort to stop the movement. Do not touch the needle bracket.
6. Tighten the fixing screw again.
7. Check if the position is correct according to the description below.
9.1.3.1 HC30TS
The setting of the horizontal movement is correct, when the opto wheel is in the
position shown in the picture, then the aspirating needle bracket is as close to
the cantilever as possible.

Figure 52
Horizontal probe mechanics
setting HC30TS
9.1.3.2 HC80TS
The setting of the horizontal movement is correct, when the opto wheel is in the
position shown in left picture, then the aspirating needle bracket is as close to
the cantilever as possible.
Figure 53
Horizontal probe mechanics
setting HC80TS
64
9.1.4 SETTING THE NEEDLE SHAFT

The needle shaft must be fastened. If it was loose it could be adjusted with the
set screw on top end of the shaft.
Figure 54
9.1.5 REMOVAL/REASSEMBLING INSTRUCTIONS OF SAMPLE ROTOR MECHANICS
9.1.5.1 Purpose of this Document

Type of procedure: Repair/service
Frequency: As needed
Sample Rotor Assembly is factory adjusted, practically maintenance-free unit.

Sample probe movement and (sample)door movement strongly depend on
correct adjustment of this unit. Do not remove and/or disassemble it unless the
driving belt or the stepper motor has to be replaced. Any other repair can be
accomplished without removal and disassembly of Sample Rotor.
9.1.5.2 List of necessary tools:

-- hex screwdriver, size: 2,5mm
-- hex screwdriver, size: 1,5 mm
-- torx screwdriver, size 10
-- Forceps

9.1.5.3 Removal and disassembling

1. Power off the unit, disconnect power connector from the back
2. Remove covers(side and front)
3. Pay attention to disconnect all cables from the covers when removing them
After removing the cover, potentially hazardous parts become

accessible, electronic boards, motors, moving parts, sampling needle,
chamber(s), tubes and valves.
4. Locate the sample rotor assembly and identify the main components:
Figure 55
1 Belt for vertical sample

3 probe movement
2 Stepper motor for vertical
movement (not part of the
1
Sample rotor assembly)
3 Sample probe shaft upper
4
fixing socket with tensioning
screw
5 4 Sample probe holder arm

5 Wash head holder arm
6 Sample rotor door
7 Home-End optos for horizon-
6
2
tal movement
8 Sample rotor stepper motor
for horizontal movement
7
5. Disconnect cables from the home-end optosensors. Connectors are sensitive,

avoid damaging them!
6. Disconnect the sample rotor motor cable from connector C_MOTOR1 of the
Mainboard.
7. Loose the vertical belt.
66
Use a 1,5 hex screwdriver to release the tensioning screw of the sample probe
driver shaft. Unscrew it until you can move the shaft by hand 1-2 mm up and
down:
Figure 56
1
1 Vertical belt tensioning
roller
8. Open the side door and remove the probe and wash head from its holders.
Figure 57
1 Probe
2 Wash Head
9. Use a 2,5 mm hex screwdriver and remove the screw which holds the belt
tensioning roller
Figure 58

Find a safe place for the roller and screw.
10. Remove the 4 screws which hold the two needle driver shaft sockets Lift up
the shaft and place it aside. Note that the belt does not allow to completely
removing it from the unit.
Figure 59 Figure 60
11. Remove the white holder with bearings ball from the rotor mechanism. It is
located under the shaft:
Figure 61
12. The sample rotor door has to be released by unscrewing the two screws
which secure the upper socket of the door:
68
Figure 62
13. The sample rotor assembly is ready to be removed from the analyzer. 4
screws hold it to the baseplate. 3 pcs. of hex screws (size: 2,5) and 1 pc. torx
screw (size:10). Remove all 4 screws:
 
 
Figure 63 Figure 64
14. Move the sample door in middle, unlocked position and remove the sample
rotor door by lifting it up.
Figure 65

15. Remove the sample rotor assembly from the instrument referring to the
pictures:
Figure 66 Figure 67 Figure 68
16. Remove the plate with the optosensors. The two screws which hold this
plate are common for the stepper motor as well and they are longer than
the two screws on the other side.
Figure 69
17. Remove the cogwheel with clutch:

Use a 1,5 mm hex screwdriver to loose (do not take out) the screws which
hold the cogwheel to the motor shaft, then pull the cogwheel upwards. It
should slip off easily from the shaft.
Figure 70
70
18. Remove the optowheel screws and the wheel.

Figure 71
1 Screws 1
2 Optowheel 2
19. Loose motor screws until they almost released

Figure 72
1
1 Screws
20. Pull the stepper motor towards the door base (white plastic part) as much as
it goes as indicated on the picture:
Figure 73

21. Remove the door shaft, the belt and the motor cogwheel as indicated on
pictures below:
Figure 74 Figure 75
9.1.5.4 Reassembling
Correct assembling of sample rotor mechanics is crucial for smooth

functioning. Please follow the instructions closely!
1. Place the belt in the door shaft housing close to the wall as indicated on
picture and replace the shaft:
Figure 76
72
2. Position the belt on the cogwheel and the cogwheel onto the motor shaft.
Figure 77
3. Replace back motor screws. Don’t tighten them yet…

4. Please pay extra attention to the next step! Set the motor cogwheel and
door shaft in the position indicated on picture. The pin must be as close to
the corner (indicated by the arrow) as possible, somewhere between the
two signs.
Figure 78
5. Hold the motor as indicated on picture. Tighten the belt by pulling the motor
until the holes on the plate match with the free holes of the motor (indicated
by arrows). Verify if positions described in previous step are correct! Tighten
screws.
Figure 79

6. Rotate the door shaft with 180°; the pin is on the opposite side with the
corner. Refer to picture and place the optowheel as indicated there. The
yellow line between the two marked pins is parallel to the belt. Place and
fasten optowheel screws.
Figure 80
The wheel setting can be checked in another position: Rotate the wheels right
until the yellow marked pins are on one line with the two shafts (blue arrows):
Figure 81
74
7. Install back optosensors and the cogwheel:
Figure 82
8. Set the cogwheel as indicated on picture for Humacount:
Figure 83
Driving rod on cogwheel must be moved freely on the longer way between the
two pins of the optowheel.
9. Install back the Sampler unit into the system. Fix it with baseplate screws.
Figure 84 Figure 85 Figure 86

10. Connect the motor and sensor cables.

11. Install the sample rotor door back and rotate it into the sample position. –
The Sample Holder is inside position!
Figure 87
12. Install back bearings ball into the rotor mechanism
Figure 88
13. Install back the holder arm shaft. Do not fixed the Sample probe shaft fixing
sockets jet, because the next setting is very sensitive. You must install the
3rd tooth of the cogged arc to the driver cogwheel in the proper sampling
arm position. The sample door is still turned inside; sampling position and
locked:
76
Figure 89 Figure 90
14. Position the wash head holder arm so that it is as close as possible to the
door upper positioner:
Figure 91
15. Position so that you can see 3 free teeth of the cogged arc to the right from
where it meet the cogwheel. Cogwheel has to be in the closest position to
the pin (as indicated on the picture), preferably touching the pin. It is the
sample aspiration end position:
• The optowheel hole is in the left opto sensor (picture in middle);
• Rotor is inner and locked position;
• The Wash head on its arm touches the metal holder of rotor door

To perform this step shared attention is needed and might not be successful at
first try. Repeat the procedure if you feel that any of affected components (wash
head holder arm, cogged arc, cogwheel) isn’t in correct place.
16. Tighten the needle shaft tensioning screw until the shaft can’t be moved
by hand in up-down direction. DO NOT OVERTIGHTEN THE TENSIONING
SCREW!
Figure 92
17. Connect the stepper motor and optosensor cables to their corresponding
connectors.
18. Place the front panel of the analyzer near and connect the LCD, Status LED
and BLTSST cables to their sockets.
19. Turn ON the analyzer, activate Service menu, and go to Troubleshooting/
Optos.
78
20. With the positions indicated on next picture adjust the optowheel if neces-
sary until „Rotor home” sensor is OFF (square is WHITE on the screen). In this
position sample rotor door must be locked inside that means the sample
holder is under the sample probe, 3 teeth of the cogged arc must be free,
cogwheel has to touch the pin and wash head arm must be in front, closest
to the door.
Figure 93
21. Manually turn the optowheel clockwise until „Rotor end” sensor goes OFF.
Rotate the cogged arc manually until the cogwheel touch the other pin. In
this position the wash head must be above the WBC chamber, door must be
start position ( Sample tube holder is outside) and locked.
22. Run „Sample rotor test” from Service menu, and then run „Needle position
check” function to verify all positions.
23. Adjust the Measuring unit horizontally if needed.

Figure 94
24. Check the performance of parameter measurements. Run Background, con-

trols and fresh patient samples.
80
9.1.6 SETTING THE POSITION OF THE CHAMBERS

After setting the needle position, the horizontal position of the chamber bracket
should be checked. The needle must not go down exactly in the center of the
chamber. Chamber bracket can be moved left or right if necessary.
Figure 95
Chamber position setting
9.2 Sample probe

The sampling needle is assembled in the H&V moving unit and it makes the
sample transfer. Correct setting of sampling needle according to the previous
sections is necessary and very important.
9.3 Washing head

The washing head is located at the bottom of the H&V moving unit and it is for
cleaning the outer surface of the sampling needle. This washing process is made
with diluent reagent and the fluid is drained by the pump.

Figure 96
Wash head
1 Diluent inlet
2 Waste outlet
9.4 Puffer reservoir

The glass puffer reservoir is directly connected to the pressure sensor.
During measurement, there is no pump activity, so the puffer reservoir main-
tains measuring vacuum stable.
The instrument measures relative pressure so measuring vacuum is independ-
ent of atmospheric pressure.
Figure 97
Puffer reservoir
82
9.4.1 HC30TS
In HC30TS this unit includes one dilutor stepper motor. The Micro dilutor
syringe makes the aspirating while the motor moves down. The syringes are
mechanically connected with a loose mechanism, so there is a phase along the
track, where the micro dilutor doesn’t move.
Figure 98
HC30TS dilutor block
1 Micro syringe 1 4
2 Positioners
3
3 Lyse syringe
4 Dliuent syringe
9.4.1.1 Setting the dilutor mechanics

The micro-dilutor’s movement must be set by the following procedure:
1. Push the dilutor pistons up as possible.
2. Fasten the set screw of the upper positioner.
3. Fasten the lower positioner’s set screw in the position shown in picture on
the left.
The distance between the lower positioner and the fixation must be:
HC30TS = 10mm
HC80TS = 13mm

Figure 99
HC30TS dilutor mechanics
9.4.2 HC80TS
In HC80TS this unit includes two dilutor channels – one for diluent and sam-
pling, and another one for lyse and diluent reagents. There are two stepper mo-
tors, 4 opto sensors, five syringes and piston rods with gear transmission. The
Micro dilutor syringe makes the aspirating while the motor moves down. The sy-
ringes are mechanically connected with a loose mechanism, so there is a phase
along the track, where the micro dilutor doesn’t move.
Figure 100
HC80TS dilutor block
1 1 Micro syringe
4
2 Positioners
3
3 Diluent syringes
4 Lyse syringe
2
84
9.4.2.1 Setting the dilutor mechanics

The micro-dilutor’s movement must be set by the following procedure:
1. Push the dilutor pistons up as possible.
2. Fasten the set screw of the upper fixing ring.
3. Fasten the lower fixing ring’s set screw in the position shown in picture on
the left.
Figure 101
HC80TS dilutor mechanics
9.5 Measuring block
9.5.1 HC30TS
The measuring block contains all components, counting chamber, measuring
tubes, HGB head, draining tubes.
Figure 102
HC30TS measuring unit

9.5.2 HC80TS
The measuring block contains all components, counting chambers, measuring
tubes, HGB head, draining tubes.
Figure 103
HC80TS measuring unit
9.6 Pump
Pump generates regulated vacuum and drains the fluidic system. It is connected
to the MAIN board and it has its own driver circuit (Power FET). In HC80TS there
are two pumps.
The pump is maintenance free.
Figure 104
Waste and vacuum pump
86

OPERATION OF THE FLUIDIC SYSTEM 87
10 OPERATION OF THE FLUIDIC SYSTEM

This section describes the main fluidic steps of HC30TS/HC80TS measurement
cycle. The instrument’s fluidic schematics are shown in section 12.3 on page 71
of this manual. The following figures show total measurement flow diagram
and detailed descriptions of processes for understanding the fluidic system
work.
The following steps are introduced in this section:
1. Flow diagram of measurement

2. Initialization process
3. Sampling process
4. Needle washing process
5. Diluting process
6. Lysing process
7. Counting process
8. Chamber draining process
9. Shutdown process
In the detailed process description figures, the active tube is filled with black
color, while an arrow shows the direction of the flow. Moving mechanic parts
have another arrow indicating direction of movement. Only opened (On) valves
are mentioned in this section while all the other valves are closed (Off).
HC30TS/HC80TS employs software counters to estimate waste (and other rea-
gent) level. Software integrates volume of the reagents used, and gives a mes-
sage when this volume reaches the preset tank capacity.
10.1 Initialization of the Fluidic System

Fluidic initialization process performs the following steps:
-- Checking of pump and pressure sensor by generating measuring vacuum
-- Positioning all mechanical components by scanning moving range (with
opto sensors)
-- Priming of reagents
-- Cleaning of tubing & measuring chamber
-- Cleaning of aperture with high-pressure back-flush, cleaner reagent &
high-voltage burning
88
10.2 Operation of the fluidic system

The details of the operation of the instrument can help to identify the subpro-
cess which causes an error.
10.2.1 HC30TS
Figure 105 Previous RBC Diluent of standby

dilution in state in chamber
HC30TS flow diagram chamber (3mL)
Needle washing Draining
Fill up with 1mL

Chamber draining Lysing process diluent for RBC
dilution
Fill up with 1mL Generating

Dilution process
diluent for WBC measuring
(1:256000)
dilution vacuum
WBC counting
Sampling process Needle washing
process
Generating
HGB
Needle washing measuring
measurement
vacuum
Diluting process Extensive RBC

(1:160) cleaning measurement
Sampling process
Blank HGB
(from primary END
measurement
dilution)

10.2.1.1 Sampling process

The aspirating needle aspirates 25 µL (50 µL in prediluted mode) of blood sam-
ple. The Micro dilutor syringe makes the aspirating while the M3 Micro-dilu-
tor motor moves down. The syringes are mechanically connected with a loose
mechanism, so there is a phase along the track, where the micro dilutor doesn’t
move.
V2 DilWash
1
V4 DilNeedle
2
1
2 1
V3 DilChamber Figure 106
3
3
2
V5 LyseWbc DILUENT
1 2 Sampling process
3 2 1
Pressure V1 Cleaner
Meter 3
P V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
Macro Lyse
3
Micro dilutor V8 Bubble

2
Dil M3
HGB
LYSE
2
V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
V10 DrainPuffer
Sample/
M1 H motor
Pump
2 1 1 2
3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
There is also another sampling process for the second (RBC) dilution, 25 µL of
primary dilution is aspirated by the sampling needle from the chamber but it
is kept in the sampling needle during the WBC measurement and the cleaning
process.
10.2.1.2 Needle washing process

Both instruments clean the sampling needle with diluent in the washing head
after sampling. It is important to clean the outer surface of the sampling needle
to avoid inaccurate sampling. The Macro syringe doses and the pump drains the
diluent from the washing head, while sampling needle moves upwards so that
the total length of it is washed and cleaned.
This process is called total sampling needle washing, and it is mainly used after
taking primary sample from sample tube. Another process, which is washing
only a smaller part of the sampling needle, is the same but the needle does not
move in the total length. Some procedures perform this kind of sampling needle
washing.
90
Figure 107
Needle washing V2 DilWash
1
V4 DilNeedle
3
1
2
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
Macro Lyse
3
2
Dil M3
HGB
LYSE
2
V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
3

M1 H motor
Pump
2 1 1 2 3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
The Macro syringe pushes the diluent through V4 (Off), V3 (Off), V2 (On). The
Pump aspirates the diluent from the washing head through V10(On), while the
M2 Vertical motor moves the sampling needle up.
10.2.1.3 Diluting process

The parts of the fluidics are rinsed with diluent reagent. The measuring chamber
is filled up with 1 ml of diluent. This method prevents the chamber from dirt and
makes the diluting process faster.
The sampling process has aspirated 25 µL of sample, which is in the sampling

needle. In the first diluting step the sample is dispensed into the measuring
chamber with 3 ml of diluent, which comes from the Macro syringe through
V4 (On) and Micro-dilutor, while the M3 Dilutor motor moves upwards. This
process makes the 1:160 first dilution rate in the chamber.

Figure 108
V2 DilWash
Diluting process
1
V4 DilNeedle
2
1
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter 3
P V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
Macro Lyse
3

2
Dil M3
HGB
LYSE
2
V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
Sample/
V10 DrainPuffer
M1 H motor
Pump
2 1 1 2 3-way
M1 Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
The second sample – 25 µL of primary dilution – is stored in the sampling needle

during the WBC measurement and the cleaning process. The instrument makes
the second (RBC) dilution into the chamber after these processes.
10.2.1.4 Lysing process

In this step the set lysing reagent is added into the measuring chamber through
V5 (On), while the Lyse syringe moves upwards. This process makes the WBC/
HGB dilution with lyse reagent.
Figure 109
V2 DilWash
Lysing process
1
V4 DilNeedle
3
1
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
Macro Lyse
3

2
Dil M3
HGB
LYSE
2
V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1

M1 H motor
Pump
2 1 1 2 3-way
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
92
For better mixing the macro syringe pushes some air bubbles (aspirated through
the washing inlet of the chamber and V8) after the lysing process through V4
(Off), V3 (On), V9 (Off) V8 (On).
10.2.1.5 Counting process

The regulated vacuum (it is generated by the pump in the puffer reservoir) aspi-
rates the diluted sample (WBC or RBC) from the chamber through V7 (On) valve.
There is no volume limiter in the system, the instrument counts the cells for
8.5 seconds in both counting phases (WBC and RBC).
Figure 110 V2 DilWash
1
V4 DilNeedle
3
1
2
2 1
V3 DilChamber
Counting process
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
Macro Lyse
3

2
Dil M3
HGB
LYSE
2
V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1

M1 H motor
Pump
2 1 1 2
3-way
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
For noise prevention there is no mechanical or electronic activity during the

counting process and the door should be closed for better shielding.

10.2.1.6 hamber draining process

Chamber draining is made under pressure control. Pump drains chamber while
puffer reservoir and thus the pressure sensor is connected to the draining tube.
The instrument can detect the empty state of the chamber from drop of vacuum
V2 DilWash Figure 111
1
V4 DilNeedle
3
Chamber draining process
1
2
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
Macro Lyse
3

2
Dil M3
HGB
LYSE
2
V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1

M1 H motor
Pump
2 1 1 2
3-way
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
10.2.1.7 Cleaning process

The pump aspirates the cleaner through the V1 (On), V9 (On), V7 (On) valves to
puffer the cleaner reagent in the tubes between V1 and V7.
Figure 112
V2 DilWash
1
V4 DilNeedle
3
1
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure
V1 Cleaner
Meter 3
P V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
WBC
1
3
Micro Dilutor Lyse Dilutor V8 Bubble

2
M3
Macro Dilutor HGB

2
V7 DrainAperture
M2
1
LYSE Ver
2
V6 DrainChamber
2 1
1

M1 H motor
Pump
2 1 1 2 3-way
Stepper Motor 2 -way Valve
M1 Valve
Closed = Off 3
WASTE Open = On
1-3 = Off
2-3 = On
94
10.2.1.8 Shutdown process

The fluidic shutdown performs the following steps:
-- Priming chamber with reagent to avoid drying out of aperture
-- Sampling needle is positioned above counting chamber, needle up
-- Lyse syringe are positioned up
-- Diluent syringes are positioned up
-- Sample rotor moved out
10.2.1.9 Tubing scheme
MAIN Board
Figure 113
Pressure
Sensor PUFFER
V1 V2 V3 V4 V5
2 2 2 2 2
3 3 3 3
1 1 1 1 1
M D L
70u
Dilutor
11 1 1 1 1
3 3 3
2 2 2 2 2
V6 V7 V8 V9 V10
out in
Pump
D

10.2.2 HC80TS
The HC80TS fluidic system operates in two parallel pneumatic processes. The
first subsystem consists of the following components: Microdilutor, Diluent I.
dilutor, V1 valve, washing head, Pump 2. This subsystem is marked with a dot-
ted line outline in the charts. The second subsystem consists of the remaining
fluidic components. Thanks to this two parallel subsystems the measuring is
started during the chamber washing processes. The separate RBC and MIX/WBC
chambers make two different diluents mixes possible simultaneously.
MIX/WBC Needle
RBC chamber Both chambers
chamber subsystem
Figure 114
Cleaning
Sampling process HC80TS flow diagram
chambers
HGB Blank
measurement
Chamber draining Needle washing
Diluting process Diluting process

(1:160) (1:160)
Sampling process
(from primary
dilution)
Lysing process Needle washing
Dilution process Dilution process

(1 : 23000) (1 : 23000)
Generating
measuring
vacuum
WBC counting
process, HGB
measurement
RBC counting
process
Draining
Cleaning
Fast Blank
measurement
END
96
10.2.2.1 Sampling process

The aspirating needle aspirates 25 µL (50 µL in prediluted mode) of blood sam-
ple. The Micro dilutor syringe makes the aspirating while the M3 Micro-dilu-
tor motor moves down. The syringes are mechanically connected with a loose
mechanism, so there is a phase along the track, where the micro dilutor doesn’t
move.
Figure 115 V2 DilNeedle V1 DilWash
1
2 1
3
3
2
Sampling process
1
V4 DilChamber
2
P V3 Cleaner
DILUENT
Puffer 2 1
Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3
1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1
MIX
RBC
V9 WBC
3
Bubble
Diluent
Micro dilutor HGB

M3 M4 M2
LYSE Ver
2
V6 DrainChamber V8 Drainchamber
2
1
1
V11 DrainPuffer
1
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
The second sample – 35 µL of primary dilution – is made in the RBC chamber.

The instrument makes the second (RBC) dilution into the chamber after straight
after the WBC dilution.
10.2.2.2 Needle washing process

Both instruments clean the sampling needle with diluent in the washing head
after sampling. It is important to clean the outer surface of the sampling needle
to avoid inaccurate sampling.
The Macro syringe doses and the pump drains the diluent from the washing
head, while sampling needle moves upwards so that the total length of it is
washed and cleaned. This process is called total sampling needle washing, and
it is mainly used after taking primary sample from sample tube.
Another process, which is washing only a smaller part of the sampling needle,
is the same but the needle does not move in the total length. Some procedures
perform this kind of sampling needle washing.

Figure 116
V2 DilNeedle V1 DilWash
1
2 1
3
3
2
1 V4 DilChamber
3
P V3 Cleaner
DILUENT
Puffer 2 1
Reservoir
V5 LyseWBC 3
V10
1 2
1 2
3 3
1 2
CLEANER
1
MIX
RBC
V9 WBC
3
Bubble
Diluent
Micro dilutor HGB

M3 M4 M2
LYSE Ver
2
2
2
1
1
V11 DrainPuffer
1
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
10.2.2.3 Diluting process

The parts of the fluidics are rinsed with diluent reagent. The measuring chamber
is filled up with 1 ml of diluent. This method prevents the chamber from dirt and
makes the diluting process faster.
The sampling process has aspirated 25 µL of sample, which is in the sampling
needle. In the first diluting step the sample is dispensed into the measuring
chamber with 3 ml of diluent, which comes from the Diluent I. syringe through
V2 (On) and Micro-dilutor, while the M3 Dilutor motor moves upwards. This pro-
cess makes the 1:160 first dilution rate in the chamber.
98
1
2 1
3
3
Diluting process
2
1
V4 DilChamber
2
P V3 Cleaner
DILUENT
Puffer 2 1
Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3
1 2
CLEANER
1
MIX
RBC
V9 WBC
2
Bubble
Diluent
Micro dilutor HGB

M3 M4 M2
LYSE Ver
2
2
1
1
3
V11 DrainPuffer
1
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
The second sample – 25 µL of primary dilution – is made right after the first di-
lution. The second dilution is made in the RBC chamber.
10.2.2.4 Lysing process

In this step the set lysing reagent is added into the measuring chamber through
V10 (On), while the Lyse syringe moves upwards. This process makes the WBC/
HGB dilution with lyse reagent.

1
2 1
3
3
Lysing process
2
1
V4 DilChamber
3
P V3 Cleaner
DILUENT
Puffer 2 1
Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3
1 2
CLEANER
1
MIX
RBC
V9 WBC
3
Bubble
Diluent
Micro dilutor HGB

M3 M4 M2
LYSE Ver
2
2
1
1
V11 DrainPuffer
1
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE

For better mixing the macro syringe pushes some air bubbles (aspirated through
the washing inlet of the chamber and V9) after the lysing process through V4
(Off), V10 (On), V9 (Off).
10.2.2.5 Counting process

The regulated vacuum (it is generated by the pump in the puffer reservoir) as-
pirates the diluted sample (WBC or RBC) from the chamber through V11 (On)
valve. There is no volume limiter in the system, the instrument counts the cells
for 16 seconds in counting phases (WBC and RBC).
1
2 1
Figure 119
3
3
2
1
V4 DilChamber
Counting process
3
P V3 Cleaner
DILUENT
Puffer 2 1
Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3
1 2
CLEANER
1
MIX
RBC
V9 WBC
3
Bubble
Diluent
Micro dilutor HGB

M3 M4 M2
LYSE Ver
2
2
1
1
V11 DrainPuffer
1
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
For noise prevention there is no mechanical or electronic activity during the

counting process and the door should be closed for better shielding.
10.2.2.6 Chamber draining process

Chamber draining is made under pressure control. Pump drains chamber while
puffer reservoir and thus the pressure sensor is connected to the draining tube.
The instrument can detect the empty state of the chamber from drop of vacuum.
100
1
2 1
3
3
Chamber draining process
2
1
V4 DilChamber
2
P V3 Cleaner
DILUENT
Puffer 2 1
Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3
1 2
CLEANER
1
MIX
RBC
V9 WBC
2
Bubble
Diluent
Micro dilutor HGB

M3 M4 M2
LYSE Ver
2
2
1
1
3
V11 DrainPuffer
1
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
10.2.2.7 Cleaning process

The pump aspirates the cleaner through the V3 (On), V10 (On), V7 (On) valves to
puffer the cleaner reagent in the tubes between V10 and V3.
1
2 1
3
3
2
Figure 121
1
V4 DilChamber
3
Cleaning process
2
P V3 Cleaner
DILUENT
Puffer 2 1
Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3
1 2
CLEANER
1
MIX
RBC
V9 WBC
3
Bubble
Diluent
Micro dilutor HGB

M3 M4 M2
LYSE Ver
2
2
1
1
V11 DrainPuffer
1
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
M1 Valve
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
After that the Diluent 2 syringes pushes the cleaner reagent remaining in the
tube between V10 and V3 into the chamber.

10.2.2.8 Shutdown process

The fluidic shutdown performs a fluidic system cleaning with cleaner.
10.2.2.9 Tubing scheme

Figure 122
MAIN Board
V1 V2 V3 VV4
12 V5
Pressure
2 2 2 2 2
3
1
3 1 1
3
1
3
1 Sensor
M D1 D2 L
D2
MEA
PUFFER
W W
II.
out in
2 1
3
2 1
3
1
3
1
3
Pump
1 2 1 2 2 2
V6 V7 V6 V9 V10 V11
out I. in
Pump
102

Further Service options and functions 103
11 FURTHER SERVICE OPTIONS AND FUNCTIONS

For the user Service several other options and fucntions are available within the
software.
Figure 123
Saving data for review at Human can be done from different places in the soft-
ware. The function to save data is always available under Manage-Backup-Save
datafile. A USB flash memory must be connected to the instrument to save the
data. The function is accessible under:
1. Database
2. Quality control-any Lot-Database
3. Maintenance-Calibration-Database
104
11.1 Maintenance menu
11.1.1 CLEANING
Under Maintenance-Cleaning an additional function to perform 5 cycles of
cleaning is available.
Figure 124
After pressing Cleaning 5 cycles the instrument will perform these cleaning cy-
cles and the progress will be shown on the display.. The process can be stopped
by pressing Abort.
Figure 125

11.1.2 CALIBRATION
Select Maintenance-Calibration.
Figure 126
In result calculations the service calibration factors are used as the user calibra-
tion factors, so they are multiplied for each parameter:
ParameterResult = UserCalibFactor * ServiceCalibFactor * ParameterMeasured
If the user calibration factor is near the bound (0.70 or 1.30), by setting the cor-
responding service calibration factor, the user factor can be adjusted to 1.00
by pressing the button Apply user factors. Example: UserCalibFactor = 1.19 and
ServiceCalibFactor = 0.96, and UserCalibFactor = 1.00 and ServiceCalibFactor =
1.14 gives the same result for the corresponding parameter.
11.1.2.1 Apply user factors

This function is used to combine user and service calibration factors. The soft-
ware will multiply the existing factors, and move them to the Service level to set
user factors to 1.00.
106
11.1.2.2 Service
The following menu is availabel under Maintenance-Calibration-Service.
Figure 127
Factors
In this dialog the service calibration factors are shown and can be modified.
Figure 128
...

Press Accept to proceed with new settings, or Cancel to keep values unchanged.
By pressing Print the factors are printed.
Press white data field to modify a calibration factor. A numeric input screen will
show up so that you can enter values. Each change must be confirmed by press-
ing Enter. All values must be in the 0.7…1.3 range.
Figure 129
108
History
You can check the previous calibration factors with the date of change in a table
form. The information can be printed by pressing Print.
Figure 130
...
11.1.3 REAGENT STATUS

The volumes for the different reagent bottle can be set under Maintenance-Re-
agent status by pressing Volume.
Figure 131
...

11.1.3.1 Changing reagent container volume

To change the volume of a reagent bottle touch the white entry field and type
the desired number. Confirm the entered value with Enter. To save all changes
press Accept. It is only recommended to change the volume of waste to 0 in
case the waste is directly connected to a standard drain. Changing one of the
other values will result in incorrect behaviour of the instrument with respect to
the monitoring of reagent volume. As a consequence the user will be informed
incorrectly of the need to change a reagent bottle.
Figure 132
...
11.1.3.2 Measurement credits

By pressing Measuremenst credits a dialog will be opened, which allows to grant
additional measurements to the instrument. This might be useful if the service
engineer provides his own reagents for servicing tasks. At the end no reagents
from the customers stock is used and the service engineer can grant the used
number of measurements back. The service engineer must use the reagent pro-
tection key, which came with his own set of reagents.
110
Figure 133
...
11.2 Settings
Under Settings the veterinary mode can be activated and functions for the
measurements can be set by service personnel.
11.2.1 GENERAL
Select Settings-General to be able to activate the veterinary mode. Press on
the field beside Veterinary to toggle between Disabled and Enabled. Confirm
changes by pressing Accept.
Figure 134

11.2.2 MEASUREMENT
Under Settings-Measurement several functions are available for service person-
nel.
11.2.2.1 Autosample ID
The automatically generated sample ID can de defined in this dialog. Modify
the different options by pressing the white fields and enter the desired text or
parameter. An example of the currently choosen format will be shown. Confirm
all changes by pressing Accept.
Figure 135
11.2.2.2 Normal Ranges

Under Settings-Measurement-Normal ranges the result ranges accepted as nor-
mal can be modifies by the user. If it is necessary the default values can be load-
ed by pressing the button Load defaults. Confirm the reset of the normal ranges
by pressing Accept.
Figure 136
112
11.3 Special Settings Service

There will be a new “Special Settings Service” option in the menu “Settings”. This
option “Special Settings Service” will be described in detail in the following
pages.
Figure 137
Specical Settings
Service Button
11.3.1 SUBMENU “GENERAL”

1. Auto Clean Cycle: If this function is enabled the user of the analyzer will
be requested to perform an “ Auto Clean Cycle ” after 60 measurements or
once a day. For the Auto Clean Cycle the analyzer uses the HC-Clean onboard
cleaner. After the cleaning cycle the analyzer requests a new blank measure-
ment.
2. Auto Wash Head Cleaning, if this function is enabled the user of the analyzer
will be requested to perform the wash head cleaning once a week.

Figure 138
Auto Clean Cycle and Auto Wash
Head Cleaning functions
11.3.2 SUBMENU “MAINTENANCE SETTINGS”
1. Maintenance request: If this function is enabled the user of the analyzer will
be automatically requested to contact the responsible service partner (This
could be entered in the field marked with “3” as shown in the figure).
The user can ignore this request up to two months. After two months all meas-
urements will be flagged with “M” for maintenance. This could only be recov-
ered by a service technician with a performed maintenance.
For a valid maintenance it is required to perform a Service calibration with

Human Calibrator. And additionally a measure of all three levels of control with
all parameters being recalled. After this the maintenance will be valid for 6
months again.
2. Last successful calibration: The last service calibration will be displayed as

the date of the preformed maintenance.
3. Service Engineer, Telephone number and Email address: Information about

the service partner will also be displayed for the user with the maintenance
request.
114
Figure 139
Submenu Maintenance Settings
11.3.3 SUBMENU HARD CLEANING

Hard-Clean cycle: If this function is enabled the analyzer will automatically
request to perform a hard cleaning cycle each 100 measurements or once a day.
We recommend using Hypochlorite solution with a concentration of 1%.
Figure 140
Submenu Hard Cleaning

11.3.4 SUBMENU “CONTROL MEASUREMENT”

1. Control Measurement Request: If this function is enabled the user of the
analyzer will be requested to measure Controls daily. (Function 2 & 1 can
only be enabled if function 1 is enabled)
2. Control Measurement Compulsory: If this function is enabled the user of the

Analyzer will be forced to perform a QC measurement before the analyzer
allows any sample measurements.
3. 3 level QC Check: If this function is enabled the user of the analyzer will be
forced to measure all three levels of QC before the analyzer allows any sam-
ple measurements.
4. Control Material Expire: If this function is enabled the analyzer will not allow
any QC measurements with an expired control material.
5. Control parameter out of range flagging (q/Q FLAG): If this function is en-
abled, - the specific sample parameter for which the control measurement
was out of range, - will be flagged with “Q” or “q” for the QC measurement.
For example: MPV was out of range in the QC measurement and the func-
tion “control parameter out of range flagging (Q FLAG)” is enabled , in this
case the analyzer will flag MVP for the entire sample measurements with “Q”
The difference between “Q” and “q” is whether a primary measured param-
eter (WBC, RBC, MCV, RDWcv, PLT, MPV) was out of control. For the other
derived parameters a “q” flag will be shown.
6. Control parameter no target value (t flag): If this function is enabled all pa-
rameters with no target value entered in the control measurement will be
flagged with the letter “t” for this specific parameter in the sample count.
For example: For PDW-SD no target value is entered during the control
measurement and the function “Control parameter no target value (t flag)”
was enabled, the analyzer will flag PDV-SD “t” in the sample measurement.
116
Figure 141
Submenu for Control Measure-
ment
11.3.5 SUBMENU “CALIBRATOR MEASUREMENT”

1. Calibration Measurement Request: If this function is enabled the analyzer
will automatically request a calibration from the user every 6 month.
2. Calibration Measurement Compulsory: If this function is enabled the ana-
lyzer will force the user to perform a calibration before the analyzer allows
any sample measurements. The performed calibration is valid for 6 months.
3. Calibration Material Expire: If this function is enabled the analyzer will not
allow any further measurement with an invalid calibrator material.

Figure 142
11.3.6 SUBMENU “SPECIAL TUBES”

In this menu you have the possibility to adapt needle position by three profiles
(Sarstedt tube, Mini tube, Custom tube) to set up each height individually. (You
are free to adjust the sample needle height in the range of -2mm up to + 15mm)
118
Figure 143
Submenu “Special Tubes”

11.3.7 DEMO MODE

This mode allows you to demonstrate the analyzer without using reagents. This
mode is particular interesting at a fair, where you would like to show custom-
ers the software features available, without getting a series of error messages
To activate the Demo mode please connect a USB keyboard to the analyzer and
press Ctr+Alt+Del, this activates the MDA Screen.
Now please type in dontmove and press enter.
Pressing again the Ctr+Alt+Del will switch back to the normal screen.
De-activating the Demo mode is exact the same procedure.
Figure 144
120

Miscellaneous 121
12 MISCELLANEOUS
12.1 MDA screen

As part of the system all commands by the software and possible documenta-
tion are displayed on the MDA scrren. This screen appears like the command line
window on usual operating systems. The content looks similar to the following:
Date 2015/03/17
OEM Human GmbH.
Model Humacount 80TS
Serial 925001
Version 1.2.808
Compiled Mar 10 2015 17:06:37
--------------------------------------------------------
Software version: 1.2.808
CPU Vendor ID:Vortex86 SoC
SPARTAN2 parallel config ver. 1.3
SPARTAN2 parallel config ver. 1.3
PIC boot ver. 0.4
PIC main ver. 2.1
RP status read from PIC is failed
Starting loader thread
...
12.1.1 EVENT LOG

The content of this screen is like an event log and can be helpful for trouble-
shooting. To store the content of this MDA screen the following steps need to
be performed:
1. Connect an external keyboard
2. Connect a USB flash memory
3. Press Ctrl-Alt-Del on the keyboard. The MDA screen will be shown.
4. Press Ctrl-PrtScr. The information on the MDA screen will be stored as text
file on the USB flash memory.
122
12.1.2 COMMAND INPUT

Two commands may be entered while MDA screen is active
Print2File: any print command from the software will be directed to a file.
Dontmove: Entering this command will toggle between demo mode and nor-
mal mode.
12.2 USB B connector communication

The communication protocol is described in a separate document, which is
available on request.
12.3 Recommended kit of tools
-- Screwdrivers:
• Cross Slot Screwdrivers (Philips)
• Slot Screwdrivers
• Hexagon Screwdrivers (3.5, 2.5, 2.0, 1.5 mm sizes)
-- Pocket digital multimeter
-- Diagonal Cutter (plier)
-- Nipper

123
124

HUMAN
Gesellschaft für Biochemica und Diagnostica mbH
Max-Planck-Ring 21 • 65205 Wiesbaden • Germany
Tel.: +49 6122/9988 0 • Fax: +49 6122/9988 100
eMail: human@human.de • www.human.de

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HumaCount 30 TS and 80TS

Cat No. 16420/802

SERVICE UND SUPPORT

3 INSTALLATION OF THE INSTRUMENT 11

4 REMOVAL OF THE INSTRUMENT 13

9 MECHANIC AND FLUIDIC PARTS 57

9.2 SAMPLE PROBE 80

10 OPERATION OF THE FLUIDIC SYSTEM 87

11 FURTHER SERVICE OPTIONS AND FUNCTIONS 103

1.2 User Warranty

1.3 Intended Use of the Instrument

1.4 General Safety Warnings

1.5 Disposal Management Concept

HumaCount 30TS and 80TS | Service manual

1.6 Biohazard Warning

1.7 Instrument Disinfection

-- For all the operations described in this manual, wear latex

HumaCount 30TS and 80TS | Service manual

HC30TS – stands for HumaCount 30TS

2.1 Name and serial number

2.2 Integrated software

HumaCount 30TS and 80TS | Service manual

3 INSTALLATION OF THE INSTRUMENT

HumaCount 30TS and 80TS | Service manual

4 REMOVAL OF THE INSTRUMENT

HumaCount 30TS and 80TS | Service manual

5.1 Self test

5.2 Device statistics

5.3 Cleaning and greasing dilutor block

5.4 Checking and lubricating dilutor piston tips

5.5 Micro piston sealing

5.6 Checking and replacing Washing Head

5.7 Bleaching of fluidic system

HumaCount 30TS and 80TS | Service manual

6.1 Export Log file

6.2 Self test

6.2.1 SELFTEST SCREENS

HumaCount 30TS and 80TS | Service manual

6.2.2 ACTIONS IN CASE OF FAILED SELF TEST PARAMETERS

Vacuum High Pump or sensor failure or Check function of pump and

Fast blank measure High Contaminated system. Run cleaning cycle

HumaCount 30TS and 80TS | Service manual

6.3 Device information

6.5 Log in as SERVICE

2. Press Logout (USERNAME).

HumaCount 30TS and 80TS | Service manual

6. Press Accept button in Log in screen.

HumaCount 30TS and 80TS | Service manual

6.6 Service Menu

6.6.1 TROUBLESHOOTING MENU

HumaCount 30TS and 80TS | Service manual

HumaCount 30TS and 80TS | Service manual

6.6.1.4 Amp. Offset

6.6.2 STRESS TEST

While Stress test is running the progress will be displayed.

HumaCount 30TS and 80TS | Service manual

6.6.3 NEEDLE POSITION CHECK

6.7 Amplifier setting

6.7.1 SETTING RBC AND WBC AMPLIFIER GAIN

6.8 Possible causes of noise

HumaCount 30TS and 80TS | Service manual

6.8.1 CONTAMINATED REAGENT

How can a good reagent become bad with time?

3 INSTALLATION OF THE INSTRUMENT 11

4 REMOVAL OF THE INSTRUMENT 13

9 MECHANIC AND FLUIDIC PARTS 57

9.2 SAMPLE PROBE 80

10 OPERATION OF THE FLUIDIC SYSTEM 87

11 FURTHER SERVICE OPTIONS AND FUNCTIONS 103