Beruflich Dokumente
Kultur Dokumente
Host Transmission
Specification
N E C M u ltiS y n c L C D 1 7 0 0 m +
GEN• S
Manufactured by
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
WARNINGS AND PRECAUTIONS
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING
TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL
INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURER’S RECOMMENDATIONS. IF IN DOUBT AS
TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY
STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO,
PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR
ANY OTHER AUTOMATED LABORATORY ANALYZER.
CAUTION System integrity might be compromised and operational failures might occur if:
r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals.
r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s
computer with software authorized by Beckman Coulter.
r You install software that is not an original copyrighted version. Only use software that is an original copyrighted
version to prevent virus contamination.
IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter
distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannot
guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most
current information bulletins concerning the product. If you purchased this product from a third party and would like
further information concerning this topic, call your Beckman Coulter Representative.
REVISION STATUS
Revision B, 4/02
Software Version 2A. Derived from internal Part Number 0302000G.
Updated Section 4.3, Dilution Factor field comments.
Removed all references to “+ Subset” globally.
Added WBC Threshold Full Histogram Group Fields.
Added new Section 3.18, Collate the ToDo List.
Updated General Information Group Fields from 29 groups to 24 groups.
Added notes to Tables 4-3 and 4-15, respectively.
Updated the following tables: 4-1; 4-3; 4-6; 4-8; 4-12; 4-13; 4-21; 4-30; 4-31; 4-33; 4-39;
4-41; 4-46; 4-48; 4-52; 5-1; 5-2; 5-4. Removed NRBC note from Tables 4-24 and 4-46.
Added “Note: Parameter selection does not affect control transmissions.” to Tables 4-43;
4-44; 4-46; 4-48; and 4-50.Updated Control Information Group 34, removed reference
WBC Count Field.Updated Section 6.2, added SOH to table. Updated Section 8.1.
Revision C, 9/03
Software Version 2B. Derived from internal Part Number 0302000K. Updated the following
tables: 4-23; 4-24; 4-43; 4-4; 4-46; 4-48; 4-50.Added new section 3.19.
Revision D, 9/04
LH 700 Software Version 2B and LH 500 Software Version 2A. Derived from internal Part
Number 0302000N. Updated the following tables: 4.12; 4.20; 4.22; 4.25; 4.28; 4.30; 4.37;
5.1.
Revision E, 9/06
LH 780 Software Version 1A, LH 750 Software Version 2D and LH 500 Software Version 2A.
Derived from internal Part Number 0302000U.
Section 3.3: Added paragraph, "Timeout settings at the host … for all other responses."
Updated the following tables: 5.4.
Note: Changes that are part of the most recent revision are indicated in text by a bar in the
margin of the amended page.
This document applies to the latest software listed and higher versions. When a subsequent software version
changes the information in this document, a new issue will be released to the Beckman Coulter website. For
labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for
your instrument.
PN 4277303EC iii
REVISION STATUS
d HARDWARE INTERFACE
d High Level Protocol
d LIS COM PORT CONFIGURATION
d Figure 8.12, Space for tower-type computers
d Figure 8.13, Space for Small Form Factor (SFF)-type computers
Note: Changes that are part of the most recent revision are indicated in text by a bar in the
margin of the amended page.
This document applies to the latest software listed and higher versions. When a subsequent software version
changes the information in this document, a new issue will be released to the Beckman Coulter website. For
labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for
your instrument.
iv PN 4277303EC
CONTENTS
CONTENTS, v
1 INTRODUCTION, 1-1
PN 4277303EC v
CONTENTS
vi PN 4277303EC
CONTENTS
7 CRC, 7-1
PN 4277303EC vii
CONTENTS
TRADEMARKS, 3
viii PN 4277303EC
Illustrations
3.1 Rear of instrument with DB 25-pin connector for tower-type computers, 3-2
3.2 Rear of instrument with DB 9-pin connector for SFF-type computers, 3-3
8.1 Plotting RBC, WBC Threshold and PLT Histograms, 8-1
8.2 Dataplot Transmission, 8-2
8.3 Dataplot Data Header, 8-2
8.4 Dataplot Data Block Format, 8-2
8.5 Dataplot Data Block Header, 8-3
8.6 Rendering Information Block, 8-3
8.7 Rendering Information Block Header Format, 8-4
8.8 Palette Table Entry, 8-4
8.9 Bitmap Format, 8-5
8.10 Dataplot Header, 8-5
8.11 Dataplot Data Format, 8-6
8.12 Space for tower-type computers, 8-6
8.13 Space for Small Form Factor (SFF)-type computers, 8-7
PN 4277303EC ix
Tables
2.1 Signal and Flow Direction for the DB 25-pin connector, 2-1
2.2 Signal and Flow Direction for the DB 9-pin connector, 2-1
3.1 Communication Parameters Available by Format, 3-1
3.2 COM Port Mapping, 3-2
3.3 Graphics Options, 3-4
4.1 Group Definition Locator, 4-3
4.2 Group Definition: 1G.1 + Retics, 4-6
4.3 General Information -- Group 1, 4-6
4.4 CBC Parameters -- Group 2, 4-9
4.5 Flag Data for CBC Parameter Group Fields, 4-10
4.6 DIFF Count Parameters -- Group 3, 4-11
4.7 Flag Data for Diff Count Parameter Group Fields, 4-12
4.8 DIFF Percent Parameters -- Group 4, 4-12
4.9 Flag Data for DIFF Percent Parameter Group Fields, 4-13
4.10 Comment -- Group 5, 4-14
4.11 Flag -- Group 6, 4-14
4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages, 4-15
4.13 Demographics -- Group 7, 4-18
4.14 RBC Histogram -- Group 11, 4-21
4.15 PLT Histogram -- Group 12, 4-21
4.16 RETIC Parameters -- Group 13, 4-22
4.17 Flag Data for Retic Parameter Group Fields, 4-24
4.18 Transmission Tests and Test IDs by Test Type, 4-25
4.19 Transmitted Groups, 4-28
4.20 General Information -- Group 1, 4-29
4.21 Possible Values for Sample Status, 4-33
4.22 CBC Parameters -- Group 2, 4-34
4.23 Flag Data for CBC Parameter Group Fields, 4-35
4.24 DIFF Count Parameters -- Group 3, 4-35
4.25 Flag Data for DIFF Count Parameter Group Fields, 4-36
4.26 DIFF Percent Parameters -- Group 4, 4-37
4.27 Flag Data for DIFF Percent Parameter Group Fields, 4-38
4.28 Comment -- Group 5, 4-38
4.29 Definitive Flags -- Group 6, 4-39
4.30 Suspect Flags -- Group 7, 4-40
4.31 Conditional Flags -- Group 8, 4-41
4.32 Other Flags -- Group 9, 4-43
4.33 Demographics -- Group 10, 4-44
4.34 RBC Histogram -- Group 14, 4-48
4.35 PLT Histogram -- Group 15, 4-48
4.36 RETIC Parameters -- Group 16, 4-49
4.37 Flag Data for Retic Parameter Group Fields, 4-50
4.38 WBC Threshold Monitor Group 26, 4-51
4.39 DIFF Latex Parameters -- Group 31, 4-51
4.40 Flag Data for DIFF Latex Parameters Group Fields, 4-52
4.41 RETIC Latex Parameters -- Group 32, 4-53
4.42 Flag Data for RETIC Latex Parameters Group Fields, 4-54
4.43 Control Information -- Group 34, 4-54
x PN 4277303EC
4.44 Control CBC Parameters -- Group 35, 4-57
4.45 Flag Data for Control CBC Parameters Group Fields, 4-58
4.46 Control DIFF Count Parameters -- Group 36, 4-58
4.47 Flag Data for DIFF Count Parameter Group Fields, 4-59
4.48 Control DIFF Percent Parameters -- Group 37, 4-60
4.49 Flag Data for Diff Percent Parameter Group Fields, 4-61
4.50 Control RETIC Parameters -- Group 38, 4-61
4.51 Flag Data for Control RETIC Parameter Group Fields, 4-62
4.52 DIFF DataPlot -- Group 50, 4-62
4.53 Retic DataPlot -- Group 51, 4-63
4.54 Research Suspect Flags -- Group 53 (LH 780 Only), 4-63
4.55 Research Population Group Fields -- Group 54 (LH 780 Only), 4-64
5.1 Message Definition Table, 5-7
5.2 Possible Values and Examples of Test Requests, 5-15
5.3 Possible Combinations of Sample Requests with Multiple Test Requests, 5-15
5.4 Patient ID Deletions, 5-17
PN 4277303EC xi
xii PN 4277303EC
1INTRODUCTION 1
1.1 PURPOSE
This document is the specification for communication between the LH Series Workstation
and a host computer. The functionality will remain backward compatible. This document is
intended for use by software engineers needing to either maintain or modify the operation of
the LH Series Workstation Host Communications.
This document describes the transfer of data from the LH Series Workstation to an LIS/HIS
computer in the following formats:
The LH 750 “STKS 1G.1 + Retics” format is provided for use with existing STKS and MAXM
interfaces. Due to the LH Series Workstation’s new features and enhancements, STKS and
MAXM instrument formats are not completely supported (see details in following sections).
PN 4277303EC 1-1
INTRODUCTION
PURPOSE
1-2 PN 4277303EC
2HARDWARE INTERFACE 2
The system is equipped with a Host connector on the rear of the LH SeriesWorkstation that
lets the system interface with a host computer via a Standard EIA-type 25-pin connector and
uses EIA Standard RS-232-C signals. The LH 500 is equipped with a 9 pin to 25 pin adapter
allowing the system to interface with a host computer. The LH 500 and LH 700 Series tower
computers must use the standard EIA-type 25 pin connector. The LH 700 Series Small Form
Factor (SFF) must use a standard EIA-type 9-pin connector. This is a Data Terminal
Equipment (DTE) configuration.
Table 2.1 Signal and Flow Direction for the DB 25-pin connector
The LH SeriesWorkstation is the DTE, by RS-232 standards, which explains the RTS/DTR
pin-outs. Whether the HOST is a DTE or DCE will determine its use of CTS/DSR or
RTS/DTR. A DTE HOST requires the use of a NULL MODEM Cable.
Pin number 5 CTS must be active for LH Series Workstation to send any transmissions. If the
host computer does not support the above hardware handshake lines, it will be necessary to
connect pin 4 to pin 5 and to connect pin 20 to pin 6.
Table 2.2 Signal and Flow Direction for the DB 9-pin connector
PN 4277303EC 2-1
HARDWARE INTERFACE
HARDWARE HANDSHAKE
The LH Series Workstation is the DTE, by RS-232 Standards, which explains the RTS/DTR
pin-outs. Whether the HOST is a DTE or DCE will determine its use of CTC/DST or
RTS/DTR. A DTE HOST requires the use of a NULL MODEM cable.
Pin 8 CTS must be active for LH Series workstation to send any transmission. It is necessary
to connect pin 7 to pin 8. The 9-pin configuration cable uses the signals listed in Table 2.2,
Signal and Flow Direction for the DB 9-pin connector. .
Note: If CTS is lowered to hold off the LH Series Workstation host transmission, it must be
raised again within the Workstation time-out period. If CTS is not raised within the time-out
period, the LH Series Workstation transmission to the host will time-out and reset, ready for
the next transmission.
2-2 PN 4277303EC
3COMMUNICATION PARAMETERS 3
3.1 COMMUNICATION PARAMETERS
The LH SeriesWorkstation allows the user to configure the following communication
parameters on the Host Computer Definition screen. These parameters include
communication modes, as well as parameters enabling the transmission of graphic data.
Note: To satisfy the host communication requirements, the receiver MUST parse for all of the
data.
PN 4277303EC 3-1
COMMUNICATION PARAMETERS
LIS COM PORT CONFIGURATION
r For the LH 700 Series tower-type computers, the default COM Port 6 must be used with
a DB 25-pin connector. See Figure 8.12, Space for tower-type computers and Figure 3.1,
Rear of instrument with DB 25-pin connector for tower-type computers.
r For the LH 700 Series Small Form Factor computers (SFF), COM Port 1 must be used
with a DB 9-pin connector. See Figure 8.13, Space for Small Form Factor (SFF)-type
computers and Figure 3.2, Rear of instrument with DB 9-pin connector for SFF-type
computers.
Refer to the following Table 3.2, COM Port Mapping for the correlation between ports and
hardware configuration.
Figure 3.1 Rear of instrument with DB 25-pin connector for tower-type computers
DB 25-pin connector
3-2 PN 4277303EC
COMMUNICATION PARAMETERS
TIME-OUT 3
Figure 3.2 Rear of instrument with DB 9-pin connector for SFF-type computers
Note: The 9-pin serial connector on the computer base, designated as COM 1, is located
beside the video display connector.
3.4 TIME-OUT
The time-out value determines the amount of time the LH SeriesWorkstation waits for a
response from the host before retrying to send data to the host. The LH SeriesWorkstation
continues to transmit at the time-out intervals until the host successfully receives the data.
The time-out values are 30, 60, and 90 seconds. The default value is 90 seconds.
Timeout settings at the host should be configured to optimize the flow of communications.
While the LH Series Workstation responds to most transmissions very quickly, allowing a
longer timeout for worst-case scenarios under heavy load conditions can help keep the
communications synchronized, and therefore maximize overall communication throughput.
We recommend allowing at least 90 seconds at the end of a host order transmission while
waiting for the DLE response, and at least 30 seconds for all other responses.
Note: The minimum recommended baud rate is 2400 for non-graphic transmissions and 9600
Baud for graphic transmissions.
3.7 PARITY
Odd, Even, and Off (None) parity modes are supported. Odd parity is the default value.
PN 4277303EC 3-3
COMMUNICATION PARAMETERS
STOP BITS
3.9 HANDSHAKE
This option determines whether or not the LH SeriesWorkstation looks for a handshake from
the host computer. See Heading 4.1, Data-Link, for additional information.
3.11 GRAPHICS
The LH SeriesWorkstation allows DIFF and Retic graphic transmission if the compatibility
format selected is the LH SeriesWorkstation format.
Table 3.3 lists the graphic items that can be enabled or disabled for the corresponding test
based on the current compatibility format and report profile selected.
Table 3.3 Graphics Options
3.12 COMPATIBILITY
This option determines which transmission format is used by the LH SeriesWorkstation to
send results to an LIS/HIS computer. The Host High Level Communication Protocols
supported are: “STKS 1G.1 + Retics” and “LH 750 Workstation”.
3.13 SPOOLER
Transmissions to the Host are spooled before sending. When a transmission is sent to the
Host, it will be kept on the Spooler and resent until the Host acknowledges that the
transmission was successfully received. If the Host is offline when Transmit To Host is
enabled, all transmissions intended for the Host will be kept on the Spooler until the Host
comes online or the Host Spooler is cleared.
3-4 PN 4277303EC
COMMUNICATION PARAMETERS
REPLACE NULL BY SP 3
3.15 REPLACE NULL BY SP
This is no longer an option because NULL characters are not included in the transmission
buffer.
PN 4277303EC 3-5
COMMUNICATION PARAMETERS
Send LH 780 Specific Fields (LH 780 Only)
3-6 PN 4277303EC
4LH SERIES WORKSTATION TO HOST COMMUNICATIONS 4
4.1 DATA-LINK
A sample transmission to the Host consists of one or more framed data blocks. See Data Block
Structure below. All transmitted data bytes are ASCII characters. All numeric values are ASCII
representations of hexadecimal numbers. Hence, for example, the number FF (hex) is
represented by the two ASCII bytes "FF" (46H, 46H). The first two bytes of the transmission
are the number of blocks to be sent, in ASCII, followed by transmission of data blocks (see
format below).
The final block will be padded with ASCII spaces (20H) to fill a whole block.
The LH Series Workstation supports “full software handshake” and “no software handshake”
protocols.
Up to 255 blocks of data can be sent. The actual number of blocks sent is specified by the
two-byte ASCII Block Count.
A SYN sent by the Host after a block has been transmitted by the LH Series Workstation forces
the Workstation to retransmit all data starting with the first block. This does not include the
PN 4277303EC 4-1
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
DATA-LINK
initial SYN and block count. It is up to the Host to determine under what conditions to
transmit a SYN.
The LH Series Workstation continues to send the same sample transmission until the Host
accepts it entirely (ACKs each block). To abort a transmission, the host must ACK each block
of the transmission and discard it locally.
No Handshake
If “Handshake” option is disabled, the protocol ignores all hardware and software host
handshake responses. In addition, in the No Handshake mode, the LH SeriesWorkstation
does not send a SYN prior to transmitting the data. CTS is always used to indicate that a Host
is MS and turned on.
NOTE: The option to turn off handshaking is suppoprted for backward compatibility. This is
not recommended as it has been shown to cause problems at slower baud rates that may
result in corrupted or last data (during high volume times).
Byte # # of bytes
1 STX 1 byte
2 BLK NBR MS CHAR 1 byte
3 BLK NBR LS CHAR 1 byte
4 DATA BYTES
•
256 (128) C
• bytes R
C
•
259 (131)
260 (132) CRC MSB MS CHAR 1 byte
261 (133) CRC MSB LS CHAR 1 byte
262 (134) CRC LSB MS CHAR 1 byte
263 (135) CRC LSB LS CHAR 1 byte
264 (136) ETX 1 byte
Each data block is framed with a Start Of Text(STX = 02H) and an End Of Text(ETX =03H)
control character.
The next two bytes after STX have the Current Block Sequence Number(BLK NBR) in ASCII
starting with the number 1(MS byte = 30H, LS byte = 31H, to give ‘01’) for the first block. The
block number of the last block must equal the Block Count sent at the beginning of the
transmission.
Every data block will have either 128 or 256 bytes (whichever is chosen). If there is
insufficient data to fill a block, it will be padded with space characters (last block
transmitted).
4-2 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
DATA-LINK 4
The CRC for the block’s data is calculated by the workstation and sent as the last four bytes of
the block before the ETX in the ASCII format shown above. The Most Significant Byte (MSB)
is generated as a Most Significant and Least Significant ASCII character (MS and LS CHAR).
The same is true for the Least Significant Byte (LSB).
The algorithm used to calculate the CRC for each block is a modified CCITT CRC16. CRC is
only calculated on the data bytes.
Chapter 7 details the CRC algorithm and includes application notes for implementing the
algorithm in both C and assembly language.
PN 4277303EC 4-3
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
Preamble
The preamble marks the beginning of a message.
C L C L C L C L C L C L C L
R F R F R F R F R F R F - - - - - - - - - - - - - - R F
Group
The DC1 (11H) character marks the beginning of a Group. There is no relationship between
the order in which the groups are defined in the following sections and the order in which
they appear after the preamble. Groups will never be omitted.
Each Group contains a DC1 (11H) character, marking the beginning of the section, a two
character field count and a variable number of fields each ending with a CR (0DH) and an LF
(0AH).
Note: When there are no data for a Group, the Group will appear as follows:
D C L
C 0 0 R F
1
Field Count
Each Group has a Field Count which identifies the number of fields contained within the
Group. Valid field counts can be from 0-255. The field count uses two characters to give the
ASCII representation of the hexadecimal value.
4-4 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
The field count will reflect the total number of fields transmitted within the Group. The field
count will always be the sum of CR/LF pairs within the Group minus one.
Fields
Each Group is made up of variable length fields which may or may not be padded with
SP (20H) characters. A field may have a variable length tag preceding the data separated with
one SP (20H) character. The data portion of a field will only contain ASCII characters in the
range of 20H to 7EH inclusive. Chapter 6 tabulates the valid ASCII characters. Fields are
separated by a CR (0DH) and an LF (0AH) character.
Note: A field's position within a Group must not be used to identify it. Fields within a Group
may appear in any order or may be omitted. The CR/LF should be used to find a field and the
Tag used to identify it.
A - Alpha characters (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H)
N - Numeric characters (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH)
X - Printable characters (20H..7EH)
Sep - Separator one or more SP characters
SP Space (ASCII 20H). Please note that all field transmissions will
include a SP between the tag and data format (this will be implied
in the following sections), as illustrated below:
Postamble
D C L C L C L
C R F R F - - - - - - - - - - - - - - R F
1
The postamble marks the end of the current message. There are 16 groups defined in the
current implementation of the “STKS 1G.1 + Retics” format. Future revisions may include
additional groups. Any future additions will not disrupt the numbering of the groups as
presently defined.
PN 4277303EC 4-5
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
Group Definition
Table 4.2 lists the groups supported by the 1G.1 + Retics format.
Table 4.2 Group Definition: 1G.1 + Retics
Group
Number Group Name 1G.1 +Retics
1 General Information X
2 CBC Parameters X
3 DIFF Count Parameters X
4 DIFF Percent Parameters X
5 Comments X
6 Flags X
7 Demographics X
8 DF1 DataPlot *
9 DF2 DataPlot *
10 DIFF (VCS) Histogram *
11 RBC Histogram X
12 PLT Histogram X
13 Retic Parameter X
14 DF5 DataPlot *
15 DF6 DataPlot *
16 Retic Histogram *
X = Supported by the LH 750, STKS 1G.1 + Retics ” format.
* = Not supported the LH 750 “STKS 1G.1 + Retics ” format. The Group is transmitted with a field count of 00.
4-6 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
Table 4.3 General Information -- Group 1
ToDo List Status C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, CDR, CR, R C, CD, R
.
ID #1 Field:
ID #2 Field:
PN 4277303EC 4-7
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
Cassette/Position Field:
Sequence Number:
1E Cassette/Position Field:
ID #1 Status Field:
4-8 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
ToDo List Status:
*Data Values:
‘GOOD’ = O.K. (Complete)
‘PART ASP’ = Partial aspiration
‘NO READ’ = Bar code not read
‘NO MATCH’ = Match not found in ToDo list
‘ ’ (20H) = Primary ID is cassette position and sample ID does not match preassigned ID
Note: On Japanese and Chinese systems, if the “Enable DBCS Transmission” option is
enabled, the characters may be in combination of single and double byte characters, using the
DBCS format.
PN 4277303EC 4-9
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
Each field in the CBC Parameters Group will have the following format:
Result Format:**
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH SeriesWorkstation.
Numeric Data: **
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following: Flag Data: **
Result Comments
..... Incomplete computation
+++++ Parameter overrange
----- Parameter voteout
The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.5.
Table 4.5 lists all the possible flags generated on the LH Series Workstation and how they
translate to the STKS “1G.1 + Retics” format.
4-10 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
Table 4.5 Flag Data for CBC Parameter Group Fields
This group is applicable to “CBC/DIFF” and “CBC/DIFF/Retic” sample runs. For all other
sample runs, this group will have a field count of 00.
Each field in the DIFF Count Parameters Group will have the following format:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data: **
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
PN 4277303EC 4-11
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
Flag Data: **
The flag data characters for the DIFF Count Parameter Group fields are as follows in
Table 4.7:
Table 4.7 Flag Data for Diff Count Parameter Group Fields
This group is applicable to “CBC/DIFF” and “CBC/DIFF/Retics” sample runs. For all other
sample runs, this group will have a field count of 00.
4-12 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
Each field in the DIFF Percent Parameters Group will have the following format:
Result Format:**
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data: **
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
+++++ Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is
greater than 600.0 x 106 cells/µL (predilute).
Flag Data: **
The flag data characters for the DIFF Percent Parameter Group fields will be as follows in
Table 4.9:
Table 4.9 Flag Data for DIFF Percent Parameter Group Fields
PN 4277303EC 4-13
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
Table 4.9 Flag Data for DIFF Percent Parameter Group Fields
The Comment Group can have a variable number of fields which consist of a variable number
of characters. Each Comment field is made up of an ASCII string identifying the appropriate
flag.
Note: On Japanese and Chinese systems, if the “Enable DBCS Transmission” option is
enabled, the characters may be in combination of single and double byte characters, using the
DBCS format.
Note: This group will always be transmitted and will only be sent once per sample
transmission.
The Flag Group can have a variable number of fields that consist of a variable number of
bytes. There are no Tags associated with a Flag field. Each Flag field is made up of an ASCII
string identifying the appropriate flag.
4-14 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
Table 4.12 lists all the possible flags generated on the LH Series Workstation and how they
translate to the “STKS 1G.1 + Retics” format. For example, a Low Event # suspect flag
message in LH 750 format is a Review message and an Abnormal WBC Pop message in “STKS
1G.1 + Retics ” format.
Suspect Flags
Low Event # Review Slide Abnormal WBC Pop
Verify Diff Review Slide Abnormal WBC Pop
Ne Blasts Blasts Abnormal WBC Pop
Ly Blasts Blasts Abnormal WBC Pop
Mo Blasts Blasts Abnormal WBC Pop
Imm. NE 1 Imm Grans/Bands 1 Abnormal WBC Pop
Imm. NE 2 Imm Grans/Bands 2 Abnormal WBC Pop
Variant LY Variant Lymphs Abnormal WBC Pop
NRBC NRBCs Abnormal RBC Pop
WBC Interference (N/A) (N/A)
RBC Interference (N/A) (N/A)
Dimorphic Reds Dimorphic RBC Pop Abnormal RBC Pop
Red Cell Agglutination RBC Agglutination Abnormal RBC Pop
Verify Retic (N/A) Verify Retic
Abnormal Retic Pattern (N/A) (N/A)
Platelet Clumps Platelet Clumps Abnormal PLT Pop
Giant Platelets Giant Platelets Abnormal PLT Pop
WBC Exceeds Linearity (N/A) (N/A)
Definitive Flags
Leukopenia Leukopenia Abnormal WBC Pop
Leukocytosis Leukocytosis Abnormal WBC Pop
Neutropenia % Neutropenia % Abnormal WBC Pop
Neutropenia # Neutropenia # Abnormal WBC Pop
Neutrophilia % Neutrophilia % Abnormal WBC Pop
Neutrophilia # Neutrophilia # Abnormal WBC Pop
Lymphopenia % Lymphopenia % Abnormal WBC Pop
Lymphopenia # Lymphopenia # Abnormal WBC Pop
Lymphocytosis % Lymphocytosis % Abnormal WBC Pop
Lymphocytosis # Lymphocytosis # Abnormal WBC Pop
Monocytosis % Monocytosis % Abnormal WBC Pop
PN 4277303EC 4-15
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
4-16 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
Table 4.12 LH 750 Workstation/STKS 1G.1 + Retics Messages (Continued)
Parameter Flags
PN 4277303EC 4-17
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
STKS Messages
PCT IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
PDW IS FOR RESEARCH ONLY, NOT FOR DIAGNOSTIC PROCEDURES
Note: These flags will be produced only for the research parameters that are transmitted.
Note: This group will be transmitted only once per sample transmission.
4-18 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
Date of Birth Field:
Sex Field:
Location Field:
PN 4277303EC 4-19
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
Physician Field:
Note: On Japanese and Chinese systems, if the “Enable DBCS Transmission” option is
enabled, the characters may be in combination of single and double byte characters, using the
DBCS format.
4-20 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
DIFF (VCS) Histogram Group Fields
The 1G.1 + Retics format does not support transmission of this group. This group will be
transmitted with field count of 00.
If RBC histogram graphic transmission is disabled, this group is transmitted with a field of 00.
If PLT histogram graphic transmission is disabled, this group is transmitted with a field of 00.
Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field is
always sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter
sent in the CBC Parameter Group.
PN 4277303EC 4-21
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
4-22 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT 4
Retic Run Time Field:
Retic ID Field:
Result Format:**
1. The parameter result will be transmitted in the Reporting Units with the extra digit
criteria selected on the LH Series Workstation.
PN 4277303EC 4-23
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
1G.1 + RETICS FORMAT
Numeric Data: **
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
????? Invalid data: could not calculate RET#.
+++++ Retic parameter result overrange
RET# > 999.9 x 106cells/µL
RET% > 100%
Flag Data: **
The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.17:
If a Retic parameter is disabled, results for that parameter will NOT be transmitted. The IRF
parameter result is transmitted with the label MI for backward compatibility reasons.
4-24 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
RETIC Histogram Group
1G.1 + Retics format does not support transmission of this group. This group will be
transmitted with field count of 00.
C L C L C L C L C L C L C L
R F R F R F R F R F R F - - - - - - - - - - - - - - R F
Transmission Identification:
TRANSMISSION TEST COUNT defines the number of tests that will be transmitted in this
particular transmission. Not all Test Types are sent in all transmissions.
Each Transmission Test is identified by the order in which it appears after the preamble. The
transmission test will never be transmitted out of order.
The Transmission Tests are up to 32 characters long. Table 4.18 lists the available
Transmission Tests and Test IDs by Test Type.
Table 4.18 Transmission Tests and Test IDs by Test Type
PN 4277303EC 4-25
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Test Identification:
T TEST C L GROUP C L
ID R F COUNT R F
GROUP COUNT defines the number of groups of data in this particular TEST Identification.
Group:
The ASCII character "G" marks the beginning of a group. A group follows the test
identification fields.
FIELD COUNT defines the number of fields in a particular group. See Field Count.
Note: When there is no data for a Group, the Group will not be sent.
Group Count:
Each test identification has a Group Count that identifies the number of groups contained
within the test. Valid group counts can be from 0-255. The group count uses two characters
to give the ASCII representation of the hexadecimal value.
Group Number:
Each group identifier has a Group Number that identifies the group number contained within
4-26 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
the group identifier. Valid group number can be from 0-255. The group number uses two
characters to give the ASCII representation of the hexadecimal value.
Field Count:
Each Group has a Field Count that identifies the number of fields contained within the
Group. Valid field counts can be from 0-255. The field count uses two characters to give the
ASCII representation of the hexadecimal value.
Fields:
Each Group is made up of variable length fields which may or may not be padded with SP
(20H), or HT (09H) characters. A field may have a variable length tag preceding the data,
separated by one SP (20H) character. Please note that all field transmissions will include a SP
between the tag and data format (this will be implied in the following sections), as illustrated
below:
The data portion of a field will only contain ASCII characters in the range of 20H to 7EH
inclusive. Heading 6.2, VALID HOST COMMUNICATIONS ASCII CODES identifies the valid
ASCII characters. Fields are separated with a CR (0DH) and an LF (0AH) character.
Note: A field's position within a Group must not be used to identify it. Fields within a Group
may appear in any order or may be omitted. The CR/LF should be used to find fields and the
Tag used to identify it.
A - Alpha characters (a..z and A..Z and space) or (61H..7AH and 41H..5AH and 20H)
N - Numeric characters (0..9 and + - .) or (30H..39H and 2BH 2DH 2EH)
X - Printable characters (20H..7EH)
H - Hexadecimal (0..9 and A..F)
characters
O - Other characters (CR and LF) or (0DH and 0AH)
PN 4277303EC 4-27
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Postamble:
C L C L C L
R F R F - - - - - - - - - - - - - - R F
There are 24 groups defined in the current implementation of the LH 750 Workstation
Format. Future revisions of LH Series Workstation may include additional groups. Any future
additions will not disrupt the order of the groups as currently defined.
Group Definition
The abbreviations used to identify tests the group applies to are:
C CBC
D DIFF
R RETICS
Table 4-19 lists the groups transmitted under 2A, 3A, GEN•S, and LH 750 Workstation
formats.
Table 4.19 Transmitted Groups
4-28 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Table 4.19 Transmitted Groups
PN 4277303EC 4-29
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Sample ID Field:
4-30 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Pre Assigned Sample ID Field:
Cassette/Position Field:
PN 4277303EC 4-31
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Sample Status:
Instrument ID:
Operator ID:
Dilution Factor::
4-32 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Body Fluid::
Stat::
Note: On Japanese and Chinese systems, if the “Enable DBCS Transmission” option is
enabled, the characters may be in combination of single and double byte characters, using the
DBCS format.
PN 4277303EC 4-33
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Each field in the CBC Parameters Group will have the following format:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data: **
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
4-34 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
+++++ Parameter overrange
----- Parameter voteout
Flag Data: **
The flag data characters for the CBC Parameter Group Fields are as follows in Table 4.23:
LH Series
Workstation Flags
4 (characters) Comments
R Review parameter
H Exceeds reference interval high limit
L Exceeds reference interval low limit
a H Exceeds action high limit
a L Exceeds action low limit
c H Exceeds critical high limit
c L Exceeds critical low limit
E Operator edited result
e Recalculated result from edited result
* MCV threshold failure
D Delta check failed
P Partial aspiration/blood detector disabled
+ Result exceeds linearity
V Single Aperture Voteout (For LH 500 ONLY)
v Result calculated from Single Aperture Voteout (For LH 500 Only)
Each field in the DIFF Count Parameters Group will have the following format:
PN 4277303EC 4-35
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
NOTE: NRBC# will only be present if the NRBC Suspect Flag was triggered.
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data: **
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
????? Invalid data: could not calculate DIFF#.
+++++ WBC parameter overrange or sum of DIFF# parameters is greater than 500.0 x
106 cells/µL (whole blood) or sum of DIFF# parameters is greater than 600.0 x
106 cells/µL (diluted).
Flag Data: **
The flag data characters for the DIFF Count Parameter Group fields are as follows in
Table 4.25:
Table 4.25 Flag Data for DIFF Count Parameter Group Fields
LH Series
Workstation Flags
(4 characters) Comments
R Review parameter: WBC threshold failure or WBC exceeds linearity or sum
of DIFF% results exceeds 100.
H Exceeds reference interval high limit
L Exceeds reference interval low limit
a H Exceeds action high limit
a L Exceeds action low limit
c H Exceeds critical high limit
4-36 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Table 4.25 Flag Data for DIFF Count Parameter Group Fields (Continued)
Each field in the DIFF Percent Parameters Group will have the following format
NOTE: NRBC% will only be present if the NRBC Suspect Flag was triggered.
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data: **
PN 4277303EC 4-37
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
+++++ Sum of DIFF% parameter results is not equal to 100% +0.1, or DIFF# parameters is
greater than 600.0 x 106 cells/µL (predilute).
Flag Data: **
The flag data characters for the DIFF Percent Parameter Group fields will be as follows in
Table 4.27:
Table 4.27 Flag Data for DIFF Percent Parameter Group Fields
LH Series
Workstation Flags
(4 characters) Comments
R Review parameter
H Exceeds reference interval high limit
L Exceeds reference interval low limit
a H Exceeds action high limit
a L Exceeds action low limit
c H Exceeds critical high limit
c L Exceeds critical low limit
E Operator edited result
e Recalculated result from edited result
D Delta check failed
P Partial aspiration/blood detector disabled
+ Result exceeds linearity
Note: This group will be transmitted only once per sample transmission.
4-38 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
The Comment Group can have a variable number of fields which consist of a variable number
of bytes. Each Comment field is made up of an ASCII string identifying the appropriate flag.
When WBC correction is performed, the Uncorrected WBC will be printed at the beginning
of the Comments field as UWBC=XXXX. For non-English applications, the UWBC will be
translated into the language being used. (For LH 750 ONLY)
Note: On Japanese and Chinese systems, if the “Enable DBCS Transmission” option is
enabled, the characters may be in combination of single and double byte characters, using the
DBCS format.
Note: This group will always be transmitted and will only be sent once per sample
transmission.
PN 4277303EC 4-39
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Note: This group will always be transmitted and will only be sent once per sample
transmission.
Note: When the LH System generates a “Cellular Interference” suspect message, the Host
transmission will replace the message with “WBC Interference”.
4-40 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
** The possible Suspect flags generated on the LH Series Workstation are:
Note: This group will be transmitted with the first test only in each sample transmission.
PN 4277303EC 4-41
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Completed Report
Blood Detector Disabled
Sample validated
Sample not validated
AutoValidated
AutoValidation:
The auto validation flags show the validation status of test requests for a sample on printouts
and within the results transmission. A functional example of the validation codes is
described below.
A specimen is run in CDR mode with a CDR report format requested. Suppose
results are acceptable for CBC and Retic, but not for the Diff (e.g., a Decision
Rule fails, such as “If Blast [suspect flag], Perform Manual Diff”).
Note: The configuration of the Workstation affects the validation messages and
codes.
The CBC and Retic test groups are AUTOVALIDATED, but the Diff group is NOT
VALIDATED. There are two ways to indicate this. A conditional text message
SAMPLE NOT VALIDATED would be transmitted. Additionally, validation
codes C AV, R AV, D NV indicate the status of the individual test groups. The Diff
can later be validated manually, resulting in
SAMPLE VALIDATED, C AV, R AV, D V.
It is possible through use of the report format to have validation messages based on the report
profile (report format). For example, using the scenario above, if a CBC report profile was
requested, validation would be based solely on the results of the CBC group. For a complete
explanation, refer to the LH Series Workstation on-line help.
Validation Flags
AV AutoValidated
NV Not Validated
V Validated (manually)
Validation Codes
C CBC parameter block
D Diff parameter block
H H&H parameter block
R Retic parameter block
W WBC/Hgb/Plt parameter block
4-42 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
If “Enable Validation Codes” in Sytem Setup - [Communications] is checked (as described in
Heading 3.17, ENABLE VALIDATION CODES), the individual test group validation status
will be transmitted based on the profile selected and test mode run. The following columns
display the possible codes transmitted for the individual groups:
Note: This group will be transmitted only once in each sample transmission.
PN 4277303EC 4-43
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Note: These flags will be produced only for the research parameters that are transmitted.
Demographics
Table 4.33 identifies the field names, test types, and applicable format of Demographics
Group Field transmissions.
Note: This group will be transmitted only once per sample transmission.
4-44 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Date of Birth Field:
Sex Field:
Location Field:
PN 4277303EC 4-45
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Physician Field:
Patient Identification:
4-46 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Patient First Name:
Sequence Number:
Profile Selection:
PN 4277303EC 4-47
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Note: On Japanese and Chinese systems, if the “Enable DBCS Transmission” option is
enabled, the characters may be in combination of single and double byte characters, using the
DBCS format.
If CBC histograms graphic transmission is disabled, this group will NOT be transmitted.
If CBC histograms graphic transmission is disabled, this group will NOT be transmitted.
Note: When the PLT histogram graphic transmission is enabled the data in the PLTF field is
always sent. Ignore the data sent in the PLTF field if an R flag is present for the PLT parameter
sent in the CBC Parameter Group.
4-48 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
PLT Fit Histogram Field:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data: **
PN 4277303EC 4-49
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
????? Invalid data: could not calculate RET#.
+++++ Retic parameter result overrange
RET# > 999.9 x 106 cells/µL
RET% > 100%
Flag Data: **
The flag data characters for the Retic Parameter Group fields will be as follows in Table 4.37:
LH Series
Workstation Flags
(4 characters) Comments
R Review parameter
H Exceeds reference interval high limit
L Exceeds reference interval low limit
a H Exceeds action high limit
a L Exceeds action low limit
c H Exceeds critical high limit
c L Exceeds critical low limit
E Operator edited result
e Recalculated result from edited result
D Delta check failed
P Partial aspiration/blood detector disabled
+ Result exceeds linearity
v Result calculated from Single Aperture Voteout (For LH 500 ONLY)
MRV, MSCV, HLR%, HLR# , IRF, RDWRSD, RDWRCV, and RSF results will be transmitted
only if they have been enabled. If a Retic parameter is disabled, results for that parameter will
NOT be transmitted.
The LH Series Workstation does not support transmission of MI field. This field will NOT be
transmitted.
4-50 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
WBC Threshold Group Fields
Table 4.38 identifies the field names, test types, and applicable format of the WBC Threshold
Monitor Group Field transmissions.
Table 4.38 WBC Threshold Monitor Group 26
If CBC histograms graphic transmission is disabled, this group will NOT be transmitted.
PN 4277303EC 4-51
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Each field in the DIFF Latex Parameters Group will have the following format:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data: **
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
Flag Data: **
The flag data characters for the DIFF Latex Parameter Group fields will be as follows as in
Table 4.40:
Table 4.40 Flag Data for DIFF Latex Parameters Group Fields
LH 750 Workstation
Flags (1 character) Comments
H Greater than expected value range.
L Less than expected value range.
4-52 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
RETIC Latex Parameters Group Fields
Table 4.41 identifies the field names, test types, and applicable format of RETIC Latex
Parameters Group transmissions.
Table 4.41 RETIC Latex Parameters -- Group 32
Each field in the RETIC Latex Parameters Group will have the following format:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data:**
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
Flag Data: **
PN 4277303EC 4-53
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
The flag data characters for the RETIC Latex Parameter Group fields will be as follows as in
Table 4.42:
Table 4.42 Flag Data for RETIC Latex Parameters Group Fields
LH Series Workstation
Flags (1 character) Comments
H Greater than expected value range.
L Less than expected value range.
Table 4.43 identifies the field names, test types, and applicable format of Control Information
Group transmissions.
The LH Series Workstation does not support transmission of this field. This field will NOT be
transmitted.
4-54 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
User Control Name Field:
Operator Field:
PN 4277303EC 4-55
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
IQAP ID Field:
Shift Field:
Cassette/Position Field:
4-56 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Reference WBC Count Field:
Each field in the Control CBC Parameters Group will have the following format:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
PN 4277303EC 4-57
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Numeric Data:**
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
----- Total voteout
+++++ Overrange
Flag Data: **
The flag data characters for the Control CBC Parameter Group fields will be as follows as in
Table 4.45:
Table 4.45 Flag Data for Control CBC Parameters Group Fields
LH Series
Workstation Flags
(4 characters) Comments
R Review parameter.
H Exceeds expected range high limit
L Exceeds expected range low limit
* MCV threshold failure
P Partial aspiration/blood detector disabled
+ Result exceeds linearity
4-58 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Each field in the Control DIFF count Parameters Group will have the following format:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data:**
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
Flag Data: **
The flag data characters for the DIFF Count Parameter Group fields will be as follows in
Table 4.47:
Table 4.47 Flag Data for DIFF Count Parameter Group Fields
LH 700 Series
Flags (4 characters) Comments
R Review parameter.
H Exceeds expected range high limit
L Exceeds expected range low limit
P Partial aspiration/blood detector disabled
+ Result exceeds linearity
PN 4277303EC 4-59
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Each field in the Control DIFF Percent Parameters Group will have the following format:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data:**
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
+++++ Sum of DIFF% parameter results not equal to 100% +0.1
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
Flag Data: **
The flag data characters for the DIFF percent Parameter Group fields will be as follows in
Table 4.49:
4-60 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Table 4.49 Flag Data for Diff Percent Parameter Group Fields
LH 700
Workstation Flags
(4 characters) Comments
R Review parameter.
H Exceeds expected range high limit
L Exceeds expected range low limit
P Partial aspiration/blood detector disabled
Each field in the Control RETICS Parameters Group will have the following format:
Result Format: **
The parameter result will be transmitted in the Reporting Units with the extra digit criteria
selected on the LH Series Workstation.
Numeric Data:**
PN 4277303EC 4-61
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
If the numeric data of each format (for example, xx.xx) does not contain a decimal number,
then it contains one of the following:
Result Comments
..... Incomplete computation
????? Invalid data: could not calculate RET#.
+++++ Retic parameter result overrange
RET# >999.9 x 106cells/µL
RET% >100%
::::: Flow cell clogged (Full clog, Partial clog 1 or Partial clog 2)
Flag Data: **
The flag data characters for the Control RETIC Parameter Group fields will be as follows in
Table 4.51:
Table 4.51 Flag Data for Control RETIC Parameter Group Fields
LH 750
Workstation Flags
(4 characters) Comments
R Review parameter.
H Exceeds expected range high limit
L Exceeds expected range low limit
P Partial aspiration/blood detector disabled
DIFF Graphics
Table 4.52 identifies the field names, test types, and applicable format of DIFF Graphics
Group Field transmissions.
4-62 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
Retic Graphics
Table 4.53 identifies the field names, test types, and applicable format of Retic Graphics
Group Field transmissions.
Table 4.53 Retic DataPlot -- Group 51
Research Suspect
Table 4.54 identifies the field name, test type and applicable format of Research Suspect Flag
transmissions.
Table 4.54 Research Suspect Flags -- Group 53 (LH 780 Only)
Note: This group will always be transmitted and will only be sent once per sample
transmission.
** The possible Research Suspect flags generated on the LH Series Workstation are:
PN 4277303EC 4-63
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
Research Population
Table 4.55 1dentifies the field name, test type and applicable format of Research Population
Group fields.
Table 4.55 Research Population Group Fields -- Group 54 (LH 780 Only)
Field Name Test Type LH 780
NEVOLMN CD, CDR CD, CDR
NEVOLSD CD, CDR CD, CDR
NECONDMN CD, CDR CD, CDR
NECONDSD CD, CDR CD, CDR
NESCATMN CD, CDR CD, CDR
NESCATSD CD, CDR CD, CDR
LYVOLMN CD, CDR CD, CDR
LYVOLSD CD, CDR CD, CDR
LYCONDMN CD, CDR CD, CDR
LYCONDSD CD, CDR CD, CDR
LYSCATMN CD, CDR CD, CDR
LYSCATSD CD, CDR CD, CDR
MOVOLMN CD, CDR CD, CDR
MOVOLSD CD, CDR CD, CDR
MOCONDMN CD, CDR CD, CDR
MOCONDSD CD, CDR CD, CDR
MOSCATMN CD, CDR CD, CDR
MOSCATSD CD, CDR CD, CDR
EOVOLMN CD, CDR CD, CDR
EOVOLSD CD, CDR CD, CDR
EOCONDMN CD, CDR CD, CDR
EOCONDSD CD, CDR CD, CDR
EOSCATMN CD, CDR CD, CDR
EOSCATSD CD, CDR CD, CDR
RETVOLMN CDR, CR, R CDR, CR, R
RETVOLSD CDR, CR, R CDR, CR, R
RETCONDMN CDR, CR, R CDR, CR, R
RETCONDSD CDR, CR, R CDR, CR, R
RETSCATMN CDR, CR, R CDR, CR, R
4-64 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
RETSCATSD CDR, CR, R CDR, CR, R
NONRETVOLMN CDR, CR, R CDR, CR, R
NONRETVOLSD CDR, CR, R CDR, CR, R
NONRETCONDMN CDR, CR, R CDR, CR, R
NONRETCONDSD CDR, CR, R CDR, CR, R
NONRETSCATMN CDR, CR, R CDR, CR, R
NONRETSCATSD CDR, CR, R CDR, CR, R
PN 4277303EC 4-65
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
4-66 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
PN 4277303EC 4-67
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
4-68 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT 4
PN 4277303EC 4-69
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
LH 750 WORKSTATION FORMAT
4-70 PN 4277303EC
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
HOST PARSING OF TRANSMITTED DATA 4
4.4 HOST PARSING OF TRANSMITTED DATA
Beckman Coulter reserves the right to make additions, deletions, and/or change the order of
parameters in future releases. However, if the communications protocol is adhered to as
specified in this document, future releases will not require reprogramming on the part of the
host.
Note that each information item is an ASCII character string. Because of delimiters, field
terminators and so on, these information strings may be extracted from the transmitted data
block without having to identify the strings at that time.
PN 4277303EC 4-71
LH SERIES WORKSTATION TO HOST COMMUNICATIONS
HOST PARSING OF TRANSMITTED DATA
4-72 PN 4277303EC
5HOST TO LH SERIES WORKSTATION COMMUNICATIONS 5
(HOST ToDo LIST)
5.1 DATA-LINK
Protocol
The LH Series Workstation requires full handshaking to receive data. The protocol is similar
to that used for LH Series Workstation to Host transmissions with the SYN character replaced
with the ENQ character.
The data is transmitted as a sequence of up to 255 blocks of data of 256 [or 128] bytes each.
Generally, these blocks contain 256 data bytes each, but due to the unique needs of differing
hosts, the system is configurable to allow shorter blocks with 128 data bytes. The final block
must be padded with ASCII spaces (20H) if it has less than the number of data bytes
necessary to complete.
ENQ
ENQ/SYN
Send block and await response. Repeat data block and response until done.
ACK/NAK/ENQ
ENQ
“All Done”
ACK/NAK/ENQ
DLE
Accept / Reject
PN 4277303EC 5-1
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
DATA-LINK
The LH Series Workstation ACKs or NAKs each block sent by the Host. If the LH Series
Workstation NAKs a block, the Host must retransmit that block. If the Workstation NAKs the
same block 3 times in a row, the Workstation aborts and is then prepared to receive the next
transmission. If the LH Series Workstation sends an ENQ after the reception of any block, the
Host must retransmit all blocks starting with the first, but should not resend the initial ENQ
character. Some common reasons why the LH Series Workstation would NAK a block are: bad
block sequence number, incorrect block byte count, CRC mismatch, etc. If the Data Link
transmission successfully completes, the LH Series Workstation then transmits a DLE
character followed by a single ASCII character indicating whether the transmission is
accepted or rejected at the presentation level. The ASCII characters following the DLE are
listed below:
# of data blocks
(2 bytes)
Data
Block
5-2 PN 4277303EC
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
DATA-LINK 5
•
•
•
ENQ (all done)
Return Codes
For the Delete request, codes A, B, C, and D still apply. In addition to these, the following
codes are returned for the Delete Function only:
PN 4277303EC 5-3
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
PRESENTATION
Byte # # of bytes
1 STX 1 byte
2 BLK NBR MS CHAR 1 byte
3 BLK NBR LS CHAR 1 byte
4 DATA BYTES
•
256 (128) C
• bytes R
C
•
259 (131)
260 (132) CRC MSB MS CHAR 1 byte
261 (133) CRC MSB LS CHAR 1 byte
262 (134) CRC LSB MS CHAR 1 byte
263 (135) CRC LSB LS CHAR 1 byte
264 (136) ETX 1 byte
5.2 PRESENTATION
Message Structure
The data which is blocked for transmission will consist of a sequence of ASCII lines each
terminated by a CR/LF pair. The block boundaries for the 256 [or 128] byte blocks would
have no special significance with respect to this data. The data may be thought of as a large
ASCII buffer which is being sent 256 [or 128] bytes at a time.
Preamble
Field
SOH 2-byte CR LF
num fields
“00” to “FF”
5-4 PN 4277303EC
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
PRESENTATION 5
Intro
Field
CR LF
2-byte 2-byte
record type operation
Each
Field Repeat until all fields given.
ASCII ASCII CR LF
Tag Data
The 2-byte num fields indicate the number of fields that follow the preamble field, that is, the
intro field plus all data fields.
The record type indicates the target data set, for example, "WL" for ToDo list (TDL).
The ASCII Tag is unique to each field within each record type.
DLE Responses From The Workstation And Effects After A ToDo List Download
INTRO FIELD: AD Add or Update
1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the
same Primary Identifier(s) and no samples are in the ToDo List or Completed list with
the same Patient ID.
a. Workstation Response - DLE A or DLE B
b. Effect - Add the downloaded request to the ToDo list.
2. Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the
same Primary Identifier(s) but there are sample(s) are in the ToDo List and/or
Completed list with the same Patient ID.
a. Workstation Response - DLE A or DLE B
b. Effect - Add the downloaded request to the ToDo list. Update the Last Name, First
Name, Gender and Birth Date for all samples in the ToDo List and/or Completed list
that have the same Patient ID.
3. Conditions prior to ToDo List Download - Sample(s) are in the ToDo list with the same
Primary Identifier(s) and sample(s) are in the ToDo List and/or Completed list with the
same Patient ID.
a. Workstation Response - DLE+E, or DLE+C if the workstation was configured to
replace DLE+E with DLE+C
b. Effect - Update the Last Name, First Name, Gender and Birth Date for all samples in
the ToDo list and/or Completed List that have the same Patient ID.
PN 4277303EC 5-5
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
PRESENTATION
4. Conditions prior to ToDo List Download - The downloaded ToDo list format is not
correct.
a. Workstation Response - DLE+C or DLE+D.
b. Effect - No modifications are made to the ToDo list or to the Completed list.
1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the
same Primary Identifier(s) and no samples are in the ToDo List or Completed list with
the same Patient ID. If samples are deleted or edited to remove the patient ID then the
Workstation will have to be rebooted before downloading a sample with the same patient
ID using the Unique intro tag. Logging off and back on is not sufficient to allow the reuse
of a patient ID.
a. Workstation Response - DLE A or DLE B
b. Effect - Add the downloaded request to the ToDo list.
2. Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the
same Primary Identifier(s) but there are sample(s) are in the ToDo List and/or
Completed list with the same Patient ID.
a. Workstation Response - DLE F
b. Effect - No modifications are made to the ToDo list or to the Completed list.
3. Conditions prior to ToDo List Download - Sample(s) are in the ToDo list with the same
Primary Identifier(s) and sample(s) are in the ToDo List and/or Completed list with the
same Patient ID.
a. Workstation Response - DLE F
b. Effect - No modifications are made to the ToDo list or to the Completed list.
4. Conditions prior to ToDo List Download - The downloaded ToDo list format is not
correct.
a. Workstation Response - DLE+C or DLE+D.
b. Effect - No modifications are made to the ToDo list or to the Completed list.
1. Conditions prior to ToDo List Download - No samples are in the ToDo list with the
same Primary Identifier(s) and no samples are in the ToDo List or Completed list with
the same Patient ID.
a. Workstation Response - DLE A or DLE B
b. Effect - Add the downloaded request to the ToDo list.
2. Conditions prior to ToDo List Download - No sample(s) are in the ToDo list with the
same Primary Identifier(s) but there are sample(s) in the ToDo List and/or Completed
list with the same Patient ID.
a. Workstation Response - DLE A or DLE B
5-6 PN 4277303EC
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION 5
b. Effect - Add the downloaded request to the ToDo list. Remove the Last Name, First
Name, Gender and Birth Date for all samples in the ToDo List and/or Completed list
3. Conditions prior to ToDo List Download - Sample(s) are in the ToDo list with the same
Primary Identifier(s) and sample(s) are in the ToDo List and/or Completed list with the
same Patient ID.
a. Workstation Response - DLE+E, or DLE+C if the workstation was configured to
replace DLE+E with DLE+C
b. Effect - Remove the Last Name, First Name, Gender and Birth Date for all samples
in the ToDo list and the Completed list that have the same Patient ID.
4. Conditions prior to ToDo List Download -. The downloaded ToDo list format is not
correct.
a. Workstation Response - DLE+C or DLE+D.
b. Effect - No modifications are made to the ToDo list or to the Completed list
DE in this field is a command by the host to delete one or more test entries in the LH Series
Workstation ToDo List. See Section 5.4, Delete Message, for a specification for this function.
After the Table is the list of possible fields, their field width and the appropriate ASCII Tag to
be used for transmission.
A field will have a variable length TAG preceding the data separated by one SP (20H)
character. A field tag must be transmitted with data. If a field tag is transmitted without data,
the Workstation rejects the whole sample request. If an invalid or unrecognized tag is
transmitted, the LH Series Workstation will ignore the field and go to the next field provided
the field to be ignored has the correct field format (i.e. is terminated by a CRLF).
Table 5.1 Message Definition Table
LH 750 Field
Field Name Workstation Requires
Patient ID X Test
Patient Last Name X Patient ID
Patient First Name X Patient ID
Sequence Number X Test
Birth Date X Patient ID
Sex X Patient ID
PN 4277303EC 5-7
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION
Location X Test
Physician Last Name X Test
Physician First Name X Test
User Field 1 X Test
User Field 2 X Test
User Field 3 X Test
Comments X Test
Report Profile X Test
Sample Drawn Date X Test
Sample Drawn Time X Test
Test X
Slide Request X Test
Stat * X Test
X = Supported by the LH 750 Workstation format.
* LH 780 Only
5-8 PN 4277303EC
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION 5
Patient ID Field:
Patient ID uniquely
identifies the set of
demographics for a
given patient. If new
demographic
information is sent with
a Patient ID that already
exists on the LH
Workstation, the
demographics are
treated as an update to
that patient and update
any existing samples
with the same Patient
ID, unless the Intro field
specifies "UN" (Unique)
or "RU" (Re-use). For a
complete description of
how samples are
processed with existing
demographics for each
Intro field option, see
the heading "DLE
Responses From The
Workstation And Effects
After A ToDo List
Download" in
section 5.2.
PN 4277303EC 5-9
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION
Sex Field:
5-10 PN 4277303EC
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION 5
Location Field:
Comments Field:
PN 4277303EC 5-11
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION
Profile Selection:
If the combined
Date/Time of the sample
is annotated later than
when the actual
Date/Time the sample is
received, the sample
drawn will be rejected.
(LH 500 Only)
If the combined
Date/Time of the sample
is annotated more than
30 days later than when
the actual Date/Time the
sample is received, the
sample drawn will be
rejected.
(LH 700 Series Only)
5-12 PN 4277303EC
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION 5
Sample Drawn Time Field:
If the combined
Date/Time of the sample
is annotated later than
when the actual
Date/Time the sample is
received, the sample
drawn will be rejected.
(LH 500 Only)
If the combined
Date/Time of the sample
is annotated more than
30 days later than when
the actual Date/Time the
sample is received, the
sample drawn will be
rejected.
(LH 700 Series Only)
PN 4277303EC 5-13
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION
Test Field:
Note: On Japanese and Chinese systems, if the “Enable DBCS Transmission” option is
enabled, the characters may be in combination of single and double byte characters, using the
DBCS format.
Stat Field:
5-14 PN 4277303EC
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
MESSAGE DEFINITION 5
S T X 1 byte Value: “S” indicates the
sample is to be
assigned heightened
“STAT” priority.
(LH 780 Only)
Table 5.3 Possible Combinations of Sample Requests with Multiple Test Requests
A sample request with multiple test requests for CBC+DIFF or Retic only, would appear
similar to the following:
.
.
.
“TS DIFF,123456,000101”
“TS RETIC,123,000203”
.
.
.
PN 4277303EC 5-15
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
Delete Message
Patient ID
Sample ID Number
Cassette Position
Test Type
The Sample ID, Cassette Position and Test Type are part of the Test Field. The formats for the
Patient ID and Test fields are described in 5.3 Message Definitions. The Patient ID and Test
field are the only fields of the message definition section used in the Deleted Function. All
others are ignored and need not be sent.
If the Patient ID field and the Test field are empty, the Workstation deletes all entries from
the ToDo List.
According to the above definition, these fields are empty when they have the following form:
It is possible for a pre assignment to be made to the ToDo List that has no Patient ID. If the
host requests deletion of this pre assignment, or some part of it, the transmission of the
deletion request must contain no Patient ID field. Note that this is not the same as an empty
Patient ID field. No field with an ID tag should be sent. See example 10.
On page 5-83, the Data Format of the Test field is given as “TEST,TUBE ID, CASS/POS” or
“TEST,TUBE ID,” or “TEST,CASS/POS”. The TUBE ID here is the SAMPLE ID. The commas
are required as separators. Note that a comma is required after TUBE ID even if it is not
followed by a CASS/POS. This Test Field Data Format has additional flexibility in the Delete
Function only.
5-16 PN 4277303EC
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
Delete Message 5
“,,CASS/POS” shall represent empty TEST and TUBE ID indictors.
NOTE: The last entry has the same effect as an empty Test field.
In the Delete context, an Empty Indicator is interpreted to refer to all values of that indicator
relative to the next highest indicator in the ToDo List. For example; If the TUBE ID indicator
is not empty and the TEST indicator is empty, this is interpreted to mean “delete all TEST’s
preassigned to this TUBE ID”. An empty Test field refers to all preassigned tests relative to a
given Patient ID (this is also true for the Test Field Data Format”,,”).
It should be noted again, if there is no Patient ID field, this is not the same as an empty
Patient ID field. This is interpreted as referring to a preassignment with no Patient ID.
1 ID_<CR><LF> TS_<CR>< LF> Delete all entries of all types in ToDo list.
2 ID_123456 TS_<CR><LF> Delete all Patient ID “123456” entries of all test types in
<CR><LF> ToDo list.
3 ID_<CR><LF> TS_CBC,,<CR><LF> Delete all CBC tests from all Patient ID entries in ToDo list.
4 ID_123456 TS_CBC,,<CR><LF> Delete all CBC tests from Patient ID 123456.
<CR><LF>
5 ID_123456 TS_CDR,987654,<CR>< LF> Delete CDR test from Tube ID “987654”and all Cass/Pos
<CR><LF> for Patient ID 123456.
6 ID_123456 TS_CDR,001001<CR>< LF> Delete all Patient ID “123456” entries of CDR test type in
<CR><LF> ToDo list with Cass/Pos “001001”.
7 ID_123456 TS_CBC,987654,001001 Delete all Patient ID “123456” entries of CBC test type in
<CR><LF> <CR>< LF> ToDo list with Tube ID “987654” and Cass/Pos “001001”.
8 ID_<CR><LF> TS_CBC,001001,<CR><LF> Delete CBC test for all the Tube IDs with Cass/Pos
“001001”for all Patient IDs.
9 ID_<CR><LF> * Delete all entries of all types in ToDo list.
10 * TS_ , ,<CR><LF> Delete all entries in ToDo list without Patient IDs.
11 ID_<CR><LF> TS_ ,ID12345,<CR><LF> Delete Tube ID from all test types.
PN 4277303EC 5-17
HOST TO LH SERIES WORKSTATION COMMUNICATIONS (HOST ToDo LIST)
Delete Message
5-18 PN 4277303EC
6ASCII Tables 6
6.1 7-BIT ASCII CODES
+ ) ) ) ) ) ) 0 0 0 0 1 1 1 1
* Ú ) ) ) ) ) 0 0 1 1 0 0 1 1
+
* + ) ) ) ) 0 1 0 1 0 1 0 1
Ú
7 6 5 4 3 2 1
X X X 0 0 0 0 NUL DLE SP 0 @ P ` p
X X X 0 0 0 1 SOH DC1 ! 1 A Q a q
X X X 0 0 1 0 STX DC2 “ 2 B R b r
X X X 0 0 1 1 ETX DC3 # 3 C S c s
X X X 0 1 0 0 EOT DC4 $ 4 D T d t
X X X 0 1 0 1 ENQ NAK % 5 E U e u
X X X 0 1 1 0 ACK SYN & 6 F V f v
X X X 0 1 1 1 BEL ETB ‘ 7 G W g w
X X X 1 0 0 0 BS CAN ( 8 H X h x
X X X 1 0 0 1 HT EM ) 9 I Y I y
X X X 1 0 1 0 LF SUB * : J Z j z
X X X 1 0 1 1 VT ESC + ; K [ k {
X X X 1 1 0 0 FF FS , < L \ l |
X X X 1 1 0 1 CR GS - = M ] m }
X X X 1 1 1 0 SO RS . > N ^ n `
X X X 1 1 1 1 SI US / ? O _ o DEL
PN 4277303EC 6-1
ASCII Tables
VALID HOST COMMUNICATIONS ASCII CODES
6-2 PN 4277303EC
7CRC 7
7.1 CRC ALGORITHM
The CRC algorithm used is a modified CCITT CRC16 algorithm and is the same as used in
the STKS analyzer.
CRCLSB,CRCMSB = data byte. Least significant and most significant CRC accumulator
bytes.
//------------------------------------------------------------------------------------------------------------------
at beginning,
CRCLSB = 0FFH (octal 377) (decimal 255)
CRCMSB = 0FFH (octal 377) (decimal 255)
and at end,
CRCLSB = CRCLSB XOR 0FFH
CRCMSB = CRCMSB XOR 0FFH
//------------------------------------------------------------------------------------------------------------------
PN 4277303EC 7-1
CRC
CRC EXAMPLE WRITTEN IN ASM86
;-------------------------------------------------------------------------------------------------------------------
GET_CRC_BYTES PROC
PUSH CX
POP CX
RET
GET_CRC_BYTES ENDP
;-------------------------------------------------------------------------------------------------------------------
7-2 PN 4277303EC
CRC
CRC EXAMPLE WRITTEN IN C 7
7.3 CRC EXAMPLE WRITTEN IN C
The following is an example of CRC16 code written in C.
//------------------------------------------------------------------------------------------------------------------
void calc_crc ( unsigned char DataByte )
{
DataByte ^= crcmsb;
DataByte ^= ( DataByte / 16 );
crcmsb = crclsb ^ ( DataByte / 8 ) ^ ( DataByte * 16 );
crclsb = DataByte ^ ( DataByte * 32 );
}
//------------------------------------------------------------------------------------------------------------------
PN 4277303EC 7-3
CRC
CRC EXAMPLE WRITTEN IN C
7-4 PN 4277303EC
8GRAPHIC RECONSTRUCTION 8
8.1 RBC, WBC THRESHOLD, AND PLT HISTOGRAMS
The histograms display a simple line graph which is representative of the count of events
occurring in each A/D channel (or voltage level). The histograms are transmitted as 512
ASCII bytes for RBC (256 bytes for PLT, 112 bytes for WBC and 512 bytes for WBC Full
Histogram) representing a 256-byte for RBC (128-byte for PLT, 56 bytes for WBC and 256
bytes for WBC Full Histogram) Hex array. It takes two ASCII bytes to represent one Hex byte.
Each Hex byte represents a scaled count for each channel 0-255 (14-142 for PLT). The
histograms can be constructed by plotting the count for each channel then drawing a line
between plots.
To display (or print) the histogram you must plot the channel vs. count. The channel
represents the X-axis while the count is the Y-axis (see Figure 8.1). There are 128 channels for
the platelet histogram, 256 channels for RBC, 56 channels for WBC and 256 channels for
WBC Full Histogram. The value range is 14-255 (0D-FF hex) for the platelet histogram and
0-255 (00-FF hex) for the WBC and RBC histograms.
y
C
O
U
N
T
<< x
CHANNEL
Dataplots
The dataplots consist of two major components: dataplot data and rendering information.
Taken together, these two components allow a receiving application to display the dataplots
in the same fashion as the LH Series Workstation.
The dataplot portion of the host transmission consists of a dataplot data header and one
dataplot data block.
PN 4277303EC 8-1
GRAPHIC RECONSTRUCTION
LH 750 DATAPLOT FORMAT
8-2 PN 4277303EC
GRAPHIC RECONSTRUCTION
LH 750 DATAPLOT FORMAT 8
Figure 8.5 Dataplot Data Block Header
Dataplot Type code Dataplot Type Code is an 8-bit integer which represents the
run type of the sample which produced this dataplot. It will
be one of the following values:
Rendering information
block header
Color palette
Dithered display bitmap
library
PN 4277303EC 8-3
GRAPHIC RECONSTRUCTION
LH 750 DATAPLOT FORMAT
Color Palette
Each color palette entry contains three 8-bit integers which represent the red, green and blue
values for that index as shown in Figure 8.8.
Red Component
Green Component
Blue Component
8-4 PN 4277303EC
GRAPHIC RECONSTRUCTION
LH 750 DATAPLOT FORMAT 8
Figure 8.9 Bitmap Format
Bitmap width
0 in pixels
Bitmap height
in pixels 7306002B
Each bitmap is stored as a column-major array of pixels represented as 8-bit integers. The
array contains:
Dataplot Header
The dataplot header contains instructions of how the dataplot data is to be interpreted. The
structure of the dataplot header is shown in Figure 8.10.
Dataplot data options Dataplot data options may be one of the following flags:
PN 4277303EC 8-5
GRAPHIC RECONSTRUCTION
COMPUTERS
Scatterplot
0
width
Scatterplot
height 7306003B
8.3 COMPUTERS
Suggested setting for the tower and small form factor computers. See Figure 8.12, Space for
tower-type computers and Figure 8.13, Space for Small Form Factor (SFF)-type computers.
Refer to the Instructions for Use manuals for installation specifics.
33 cm (13 in.)
CLEARANCE
44.5 cm
45.7 cm (17.5 in.)
(18 in.) 68.6 cm
(27 in.) 88.9 cm
(35 in.)
30 cm (12 in.) 47 cm
50.2 cm (18.5 in.)
CLEARANCE
(19.75 in.) 50.2 cm NEC MultiSync LCD 1700m+
(19.75 in.)
CLEARANCE LH 780
12.7 cm
(5 in.) 74.9 cm (29.5 in.) 101.6 cm (40 in.) 45.7 cm (18 in.)
61 cm 45.7 cm
(24 in.) (18 in.)
CLEARANCE CLEARANCE
43.1 cm
63.5 cm (17 in.)
(25 in.) 15 cm
(6 in.)
66 cm
(26 in.) 55.9 cm
38.1 cm
(22 in.)
(15 in.)
8-6 PN 4277303EC
GRAPHIC RECONSTRUCTION
COMPUTERS 8
Figure 8.13 Space for Small Form Factor (SFF)-type computers
CLEARANCE
30 cm (13 in.) 25.4 cm (10 in.) 38 cm (15 in.)
44.5 cm
(17.5 in.)
68.6 cm
76.2 cm (27 in.) 88.9 cm
(30 in.)
45.7 cm (35 in.) 64.8 cm
30 cm (12 in.)
(18 in.) (25.5 in.)
CLEARANCE NEC MultiSync LCD 1700m+
50.2 cm
(19.75 in.) 50.2 cm
DVD
(19.75 in.)
RW disc
CLEARANCE LH 780
64.5 cm
25.4 cm (25.4 in.)
(10 in.) 60.3 cm (23.75 in.) 74.9 cm (29.5 in.) 101.6 cm (40 in.) 45.2 cm (17.8 in.)
61 cm
(24 in.)
CLEARANCE CLEARANCE
63.5 cm
(25 in.)
66 cm
38.1 cm (26 in.)
(15 in.)
PN 4277303EC 8-7
GRAPHIC RECONSTRUCTION
COMPUTERS
8-8 PN 4277303EC
Numerics G
7-BIT ASCII CODES, 6-1 General Information Group Fields, 4-6
Graphics, 3-4
Group Definition Locator Table, 4-3
A
ASM86, 7-2
H
Hardware Handshake Mode, 2-2
B Host Communications
Block Size, 3-4 Disabling, 3-5
HOST COMMUNICATIONS ASCII CODES, 6-1
C
CBC Parameter Group Fields, 4-9 L
CBC Parameters Group Fields, 4-34 LH 750
Communication Parameters, 3-1 DATAPLOT Format, 8-1
Communication Protocol LH 780
High Level Protocol, 3-1 Specific Fields, 3-5
Communication Protocols, 3-1
Low Level Protocol, 3-1
Compatibility, 3-4 M
Control DIFF Percent Parameters Group Message Structure, 4-4
Fields, 4-60
CRC
ASM86, 7-2 N
C, 7-3 Null, 3-5
CRC ALGORITHM, 7-1
O
D Other Flags Group Fields, 4-43
Data Bits, 3-3
data block structure, 4-2 P
Data Link
Parity, 3-3
Full software handshake, 4-1
Presentation
software handshake, 4-1
Message Structure, 5-4
DATA-LINK
Protocol, 5-1
Data-Link R
Data Block Structure, 5-4 RBC Histogram Group Fields, 4-48
Delete Message, 5-16 Research Population, 4-64
Demographics, 4-44 Retic Graphics, 4-63
DIFF Count Parameters Group Fields, 4-35 RETIC Latex Parameters Group Fields, 4-53
DLE E RETIC Parameters Group Fields, 4-49
DLE C, 3-5
S
F Signal Names, 2-1
Flag Data, 4-10 Spooler, 3-4
Clearing the Spool, 3-4
STKS and MAXM, 1-1
PN 4277303EC INDEX - 1
Stop Bits, 3-4
Suspect Flags Group Fields, 4-40
T
Time-Out, 3-3
Transmitted Groups, 4-28
V
Validation Codes
Enable, 3-5
W
WBC Threshold Group Fields, 4-51
INDEX - 2 PN 4277303EC
BECKMAN COULTER, INC. CUSTOMER END USER LICENSE AGREEMENT
This Product contains software that is owned by Beckman Coulter, Inc. or its suppliers and is protected by
United States and international copyright laws and international trade provisions. You must treat the
software contained in this Product like any other copyrighted material. This license and your right to use
the Product terminate automatically if you violate any part of this agreement.
This is a license agreement and not an agreement for sale. Beckman Coulter hereby licenses this Software
to you under the following terms and conditions:
You May:
1. Use this software in the computer supplied to you by Beckman Coulter;
2. Maintain one copy of this software for backup purposes (the backup copy shall be supplied by
Beckman Coulter);
3. After written notification to Beckman Coulter, transfer the entire Product to another person or entity,
provided you retain no copies of the Product software and the transferee agrees to the terms of this
license agreement.
Limited Warranty
Beckman Coulter warrants that the software will substantially conform to the published specifications for
the Product in which it is contained, provided that it is used on the computer hardware and in the
operating system environment for which it was designed. Should the media on which your software
arrives prove defective, Beckman Coulter will replace said media free of charge within 90 days of delivery
of the Product. This is your sole remedy for any breech of warranty for this software.
Except as specifically noted above, Beckman Coulter makes no warranty or representation, either
expressed or implied, with respect to this software or its documentation including quality, performance,
merchantability, or fitness for a particular purpose.
General
This agreement constitutes the entire agreement between you and Beckman Coulter and supersedes any
prior agreement concerning this Product software. It shall not be modified except by written agreement
dated subsequent to the date of this agreement signed by an authorized Beckman Coulter representative.
Beckman Coulter is not bound by any provision of any purchase order, receipt, acceptance, confirmation,
correspondence, or otherwise, unless Beckman Coulter specifically agrees to the provision in writing. This
agreement is governed by the laws of the State ofCalifornia.
TRADEMARKS
Beckman Coulter, the stylized logo, and COULTER are trademarks of Beckman Coulter, Inc.
and are registered with the USPTO.
All other trademarks, service marks, products, or services are trademarks or registered
trademarks of their respective holders.
Made in USA
PN 4277303EC
Documentation
The information in the Reference manual, Operator’s Guide, Special Procedures and Troubleshooting manual, SlideMaker
Operator’s Guide, SlideStainer Operator’s Guide, and the Body Fluids Instructions for Use comes from the Online Help
System.