KENDRIYA VIDYALAYA NO.

1 BBSR (1ST SHIFT)

SECOND PREBOARD 2018-19
SUB: BIOTECHNOLOGY
TIME: 3 HOURS MM: 70

1. Name the company which first generated the recombinant humulin. 1

2. Write the complementary sequence of 5’ AWGDBVSH 3’ 1
3. An animal cell culture medium has turned yellow after some time of culture. Suggest 1
the possible reason.
4. Which type of proteomics must be carried out to study the structure & nature of 1
protein complexes in a cell?
5. Suggest any two applications of rHuEPO. 1
6. A researcher desires to grow seedless variety of apple. Suggest a method by which 1
he can achieve it.
7. Name and explain the two types of microbial continuous culture system. 2
8. Name the institute and it’s contributions that was established under the leadership of 2
Max Perutz & has produced nine noble prize winners.
9. a) Name the most commonly used media in plant tissue culture. 2
b) Name one cryoprotective agent and its role.
10. You have collected an animal cell line from a Culture collection centre. 2
a) How will you be benefited?
b) How will you characterize and confirm the given cell line?
11. What is germplasm? Mention any two benefits of germplasm conservation. 1+1
12. Classify the vectors which you can use for the following purpose: 2
a) To insert foreign gene of size 22kb
b) For generating ssDNA templates for DNA sequencing
c) A vector that can grow in both prokaryotes and eukaryotes.
d) An animal vector
13. Identify the mutated proteins that result in the following diseases in human: 2
a) Mad cow disease.
b) Thalassemia
c) SCID
d) Sickle cell anemia
14. a) What is the molecular basis of SNP? 2
b) What is SNP map?
c) Name the gene in which a SNP can result in AIDS resistance.
d) Write one use of SNP in molecular biology.
15. a) What is the modern method of microbial strain improvement called? 3
 b) Write any two problems associated with it and its solutions.
16. a) What is the principle of Mass Spectrometry? 1+2
b) Draw a labeled diagram of the components of MS.
17. You want to generate a large number of identical clones of a species of plant. 3
a) How can you do so?
b) You now identify a mutant out of that clone of plants. What is it called?
c) The mutant has a desirable characteristic. How can you preserve it?
18. Explain the two types of plant regeneration pathways. 3
19. a) Explain in brief any two types of gene level sequences. 2+1
b) Distinguish between homologous and paralogous sequences.
20. a) Define specific growth rate. 3
b) Calculate the specific growth rate of a microbial culture where the number of cells
has increased from 105 to 1010 in 5 hours of culture.
21. Explain the selection method which provides visual screening of recombinant and 2+1
non recombinant cells. Why is it called insertional inactivation?
22. a) Why is the technique for production of MoAB called hybridima technology? 3
b) Why are MoAb preferred over serum antibodies in diagnostics and therapeutics?
c) Give one example of MoAb with its use.
23. a) Draw the steps of tissue engineering technique. 3
b) What are the advantages of this technique?
24. Give a brief account of the different types of sequences that are submitted in 3
different databases.
Or,
Expand BLAST and describe the steps of BLAST.
25. You want to create a very specific point mutation in the gene of your interest for 3
enhancing the functional stability of its product. Name and explain the technique
with a diagram that you will use.
26. a) What are the unique properties shown by embryonic stem (ES) cells? (any five) 21/2+
b) Name the scientist who first isolated ad cultured human ES cells. 1+
c) Name one adult stem cell and its function. 11/2
27. a) Why is Agrobacterium tumefacien called as a natural genetic engineer of plants? 2+2+1
b) What is co-cultivation?
c) Name one bacteriostatic agent used after co-cultivation.
28. a) What is Philadelphia chromosome and what does it cause? 2+3
b) Name and explain the technique used to detect the disease caused by the
Philadelphia chromosome.