Beruflich Dokumente
Kultur Dokumente
PROCESSING
chromatographic
separations of life
science molecules. published by srl
Via Cesare da Sesto, 10
20123 Milano (Italy)
SUMMARY
Scale-up and
design of plant
scale column
chromatography
can follow different
Figure 5 – Peak profile obtained using a non-binding
guidelines for
tracer molecule (or during product elution)
small molecule
adsorption and
macromolecular REFERENCES
adsorption.
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diffusion limited than Transfer and Fluid Mechanics” in Scale-up
and Optimization in Preparative
small molecules, and
Chromatography; Rathore A.S., Velayudhan
hence while small A. Eds.; Chromatographic Science Series,
molecule Marcel Dekker, 2003
chromatography can be 2) CHASE H.A. “Purification of Proteins by
scaled up using simpler Adsorption Chromatography in Expanded
mean residence time Beds” Trends Biotechnol. 1994, 12,
criterion, macromolecule 296-303
behaviour during operation as well, and chromatography required more careful 3) LALI A.M. “Expanded Bed Affinity
can be used in analyzing product peak scale up. Larger the product molecule Chromatography” in Methods for Affinity
(e.g. pDNA) larger is the effect of fluid based Separations of Enzymes and
profiles.
Proteins; Gupta M.N. Ed.; Birkhauser Verlag:
In large columns use of small particle dynamics on binding capacity and peak
Basel, 2002
size adsorbent is more likely to result in resolutions. 4) RATHORE, A.S., KENNEDY R.M., O’DONNELL
variation of performance between Production scale packed and J.K., BEMBERIS I., KALTENBRUNNER O.,
subsequent packing. Hence, larger particle expanded bed adsorption columns “Quantification of a Chromatographic Column,
size may be preferred even at the expense need specific attention to design details Why and How to Do It” Biopharm
of the lower efficiency in terms of HETP. and use of methods to check and International 2003, March 30