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0 INTRODUCTION

Filtration is basically to separate solid-liquid mixture by using a membrane filtration.

There are two type of filtration which are conventional and cross flow filtration. Tangential
flow filtration (TFF) is one type of cross flow filtration. The different between these two
filtration is the flow of feed through the membrane filter. Membrane filtration is a separation
technique that widely used depending on membrane porosity, it can be classified as a
microfiltration or ultrafiltration process. In this lab, ultrafiltration is used in the TFF.

Tangential flow filtration (TFF) is a rapid and efficient method for separation and
purification of biomolecules. It can be used to fractionate large from small biomolecules,
harvest cell suspensions, and clarify fermentation broths and cell lysates (Larry Schwartz,
2018). TFF is use as fractionation of large from small biomolecules, clarification and removal
of cells as well as cellular debris from fermentation or cell culture broths (Larry Schwartz,
2018)

The basic flat sheet tangential flow unit consists of a shallow channel with rectangular
flat sheet membranes on one or both sides (Raja Ghosh, 2006). The membranes are supported
on grooved plates which allow easy collection of permeate. The feed is pumped into the channel
and permeate which crosses the membrane is collected by the grooved plates. The advantages
of the flat sheet tangential flow membrane module is the ease of cleaning and replacement of
defective membranes. To add to that, it have ability to handle viscous feeds and feed with high
levels of suspended solids. TFF membrane also can be used for ultrafiltration, microfiltration
or nanofiltration.

In this experiment, yeast suspension is use in the TFF. In the yeast suspension contains
high solid particles. Thus, using TFF it can filtrate the pure yeast liquid and can use for further
experimental usage.
OBJECTIVES

1. Study on Tangential Flow Filtration and perform the procedure.

2. Identify the components of TFF membrane filters
3. Explain the principle and operation of TFF membrane filters
4. Understand correlation of concentration polarization between flow rate and trans
membrane pressure.
REFERENCES
1) Roger G. Harisson, P. W. (2003). Bioseparations Sciences and Engineering.

2) Raja Ghosh. (2006). Principle of Bioseparations Engineering, World Scientific Publishing

Co. Pte. Ltd, Singapore

3)

Larry Schwartz, S. T. (2018). Introduction to Tangential Flow Filtration for Laboratory and
Process Development. Retrieved from Pall Corporation:
https://laboratory.pall.com/content/dam/pall/laboratory/literature-library/non-gated/id-
34212.pdf