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Names: _______________________________________ Date: __________________

Section: __________________ Laboratory Activity No.___

Introduction:

Laboratory tests contribute vital information about a patient's health. Correct diagnostic
and therapeutic decisions rely, in part, on the accuracy of test results. Adequate patient
preparation, specimen collection, and specimen handling are essential prerequisites for
accurate test results. The accuracy of test results is dependent on the integrity of specimens.

Purpose:
 To see the structure of the red blood cell in the microscope using different
mediums.

Laboratory Equipment:
Frog Lab gown Microscope slide
Fish Gloves Flashlight
Dissecting pan Mask
Microscope Needle
Procedures:

Part I: Getting fish blood


1. Have an assistant pick up the fish in both hands and hold it upside down for you.
2. Keep the body of the fish straight and the tail facing towards you.

3. Insert a syringe under the skin of the ventral midline of the caudal peduncle.
4. Use one hand to keep the tail straight. Gently push the needle under the skin of the fish
at about a 45-degree angle away from you.
5. Push the needle toward the vertebral column until it reaches the base.
6. Gently slide the needle in until you feel it hit the base of the fishes spinal column.
7. Pull it back towards you less than 1 mm so it is not against the base.
8. Be careful to stop pushing the needle as soon as you feel it hit the vertebral column
so you don’t pierce the spine of the fish.

9. Pull the plunger of the syringe to draw the blood.


10. Carefully extract a blood sample from the fish with the syringe. Pull the needle out of
the fish slowly when you have the sample.
11. Make sure your assistant is holding the fish very stable as you withdraw the blood.

12. Carefully get blood sample of the fish using the medicine dropper in the syringe.

13. See the structure of the blood in the microscope.

Part II: Getting frog blood

14. Same process just like in the fish but you can get sample of the blood into their
cardiac area, lower part of their face and posterior part of the body
15. See the structure of the blood in the microscope.
Part III: Getting human blood

16. Wash the hands before doing the activity


17. Tightly hold the wrist where you will draw the blood
18. With the use of the needle being soaked with hot water, prick the finger gently and
carefully.
19. With the use of medicine dropper get the blood sample
20. See the structure of the blood in the microscope.

Anatomy of the fish blood sample: Anatomy of the frog blood sample:

Anatom of the human blood sample: