CH62

USN 1 M S

RAMAIAH
Institute of Technology
(Autonomous Institute, Affiliated to VTU)
(Approved by AICTE, New Delhi & Govt. of Karnataka) Accredited by NBA & NAAC with ‘A’ Grade

SEMESTER END EXAMINATIONS – MAY/JUNE 2018

Course & Branch : B.E. : Chemical Engineering Semester : VI
Subject : Biochemical Engineering Max. Marks : 100
Subject Code : CH62 Duration : 3 Hrs

Instructions to the Candidates:

 Answer one full question from each unit.

UNIT- I
1. a) Explain with a neat diagram, the structure of DNA proposed by Watson CO1 (10)
and Crick.
b) Classify carbohydrates and explain in detail about aldoses and ketoses. CO1 (10)

2. a) Distinguish between prokaryotes and eukaryotes with neat diagrams. CO1 (10)
b) Write short notes on classification of lipids and their structures. CO1 (10)

UNIT – II
3. a) Discuss classification of enzymes. CO2 (10)
b) From a series of batch runs with a constant enzyme concentration, CO2 (10)
thefollowing initial rate data were obtained as a function of initial
substrateconcentration.

Evaluate the Michaelis-Menten kinetic parameters by employing the

Lineweaver-Burk plot and the Eadie-Hofstee plot.

4. a) Explain the structures of proteins with neat diagrams. CO2 (10)

b) Derive M-M kinetics using rapid equilibrium approach. CO2 (10)

UNIT- III
5. a) The following data were obtained from enzymatic oxidation of phenol CO3 (10)
by phenol oxidase at different phenol concentrations.

Page 1 of 2
 CH62
S (mg/l)  (mg/l-h)
10 5
20 7.5
30 10
50 12.5
60 13.7
80 15
90 15
110 12.5
130 9.5
140 7.5
150 5.7
i) What type of inhibition is this?
ii) Determine the MM constants and inhibition constant.
iii) Determine the oxidation rate at [S] = 70 mg/l
b) Derive rate expressions for competitive inhibition. CO3 (10)

6. a) Explain the effect of temperature on enzyme kinetics. CO3 (10)

b) When the inhibitor was added, the initial reaction rate was decreased CO3 (10)
as follows

Is this competitive inhibition or noncompetitive inhibition? Justify your

answer by showing the effect of the inhibitor graphically.

UNIT- IV
7. a) Write about various types of culture media used for cultivation of CO4 (10)
bacteria.
b) Write short notes on: CO4 (10)
i) Fluidized bed bioreactor ii) Continuous sterilization.

8. a) Give an account of design of batch sterilization equipment assuming CO4 (10)

the death rate of cells depends on temperature and follows Arrhenius
equation.
b) Explain in detail about operation and maintenance of aseptic aerobic CO4 (10)
fermentation processes.

UNIT- V
9. a) Derive an expression for optimum dilution rate. CO5 (12)
b) Explain stage wise batch production of oxytetracycline by filamentous CO5 (08)
organisms.

10. a) What are the various growth cycle phases for a batch cell cultivation. CO5 (10)
b) Discuss the steps involved in product purification . CO5 (10)
****************

Page 2 of 2