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Presence of Glucose and Protein in the Urine Samples of Alcoholics 1

CHAPTER I

THE PROBLEM

Introduction

People who are affected with alcoholism are unable to control their obsession, including

both how much they drink and when they drink. Subsequently, this can lead to health problems

and/or issues at home or at work. Plenty of lifelong alcoholics find themselves alone and in poor

health.

The kidneys take out waste material, minerals, fluids, and other substances from the blood

to be passed in the urine. Urine has hundreds of different body wastes. What you eat and drink,

how much you exercise, and how well your kidneys work can affect what is in your urine.

A urine test checks different components of urine, a waste product made by the kidneys. A

regular urine test may be done to help find the cause of symptoms. The test can give information

about your health and problems you may have.

This study determines the presence of glucose and protein in the urine samples of

alcoholics. The researchers are interested to look into the possible causes that affect the formation

of glucose and protein in the urine of alcoholics. Through this study, the researchers can help

provide insights towards the possible contributors of these phenomena. Thus, this study was

conducted.
Presence of Glucose and Protein in the Urine Samples of Alcoholics 2

STATEMENTS OF THE PROBLEM

This study aims to determine the presence of glucose and protein in the urine samples of

alcoholics.

Specifically, it seeks to answer the following questions:

1. What is the demographic profile of the alcoholics in terms of their:

1.1 sex;

1.2 age;

1.3 frequency of drinking.

2. What is the percentage in the urine samples positive with glucose and protein?

3. Is there a significant relationship between:

3.1 sex and presence of glucose or protein;

3.2 age and presence of glucose or protein;

3.3 frequency of drinking and presence of glucose or protein?


Presence of Glucose and Protein in the Urine Samples of Alcoholics 3

Scope and Delimitation

This research focused on the presence of glucose and protein in the urine samples of

alcoholics. The respondents of this study are 50 individuals, 10 females and 40 males who often

drink alcoholic beverages for at least twice a week. The time frame for the preparation of the urine

samples when the experimental data conducted was prepared at least two hours before it was tested.

Significance of the Study

The findings of the study are beneficial to the following:

Alcoholics. This study will help them identify the possible causes of the presence of glucose and

protein in the urine due to too much drinking of alcoholic drinks.

Family members of alcoholics. This study can be used in assessing the possible health problems

that the alcoholics might encounter due to too much drinking.

Researcher. This study will be a significant endeavor in identifying the possible causes of the

presence of glucose and protein in the urine of alcoholics.

Other researchers. In order for them to acquire relevant information about the presence of glucose

and protein in the urine as a tool for conducting other researches.


Presence of Glucose and Protein in the Urine Samples of Alcoholics 4

Definition of Terms

To further understand this study, the following terms are defined on how it was used in

the study.

Sex. Either of the two main categories (male and female) into which humans and most other living

things are divided on the basis of their reproductive functions.

Age. The length of time that a person has lived or a thing has existed.

Frequency. The number of occurrences of a repeating event per unit of time.

Glucose. It is a simple sugar with the molecular formula C6H12O6. Glucose is the most

abundant monosaccharide and a subcategory of carbohydrates.

Protein. Are large biomolecules, or macromolecules, consisting of one or more long chains

of amino acid residues.

Alcoholic beverage. It is a drink that contains ethanol, a type of alcohol produced

by fermentation of grains, fruits, or other sources of sugar.

Alcoholism. It is a broad term for any drinking of alcohol that results in mental or

physical health problems.


Presence of Glucose and Protein in the Urine Samples of Alcoholics 5

CHAPTER II

CONCEPTUAL FRAMEWORK

Independent Variable Dependent Variable

Too much intake Presence of


of alcoholic glucose and
drinks protein in the urine

Figure 1. Conceptual Paradigm

This study aims to determine the presence of glucose and protein in the urine samples of
terms
alcoholics.

HYPOTHESIS

There is a significant relationship between the presence of glucose and protein and urine

to too much intake of alcoholic drinks.


Presence of Glucose and Protein in the Urine Samples of Alcoholics 6

REVIEW OF RELATED LITE8RATURE

According to Delphi Behavioral Health Group, Alcoholism is the most severe form of

alcohol abuse and involves the inability to manage drinking habits. It is also commonly referred

to as alcohol use disorder. Alcohol use disorder is organized into three categories: mild, moderate

and severe. Each category has various symptoms and can cause harmful side effects. If left

untreated, any type of alcohol abuse can spiral out of control.

Individuals struggling with alcoholism often feel as though they cannot function normally

without alcohol. This can lead to a wide range of issues and impact professional goals, personal

matters, relationships and overall health. Over time, the serious side effects of consistent alcohol

abuse can worsen and produce damaging complications (Galbicsek, 2018).

A urinalysis is a simple test that looks at a small sample of your urine. It can help find

problems that need treatment, including infections or kidney problems. It can also help find

serious diseases in the early stages, like kidney disease, diabetes, or liver disease. A urinalysis is

also called a “urine test.” A urinalysis can help to detect many diseases before you feel symptoms.

Finding and treating a problem early can help keep serious diseases from getting worse.

According from the study conducted by the University of California, normally, the liver

releases glucose to maintain blood sugar levels. But when you drink alcohol, the liver is busy

breaking the alcohol down, so it does a poor job of releasing glucose into the bloodstream. This

lead to a drop in blood sugar levels if you are drinking alcohol on an empty stomach.

Each alcoholic beverage takes about 1-1 ½ hours to finish processing in the liver. For that

entire time, the risk of low blood sugar exists. So, if you have 2 drinks, you double that time to 2

to 3 hours that you are at risk for low blood sugar. The more alcohol consumed, the bigger the risk

for serious low blood sugar.


Presence of Glucose and Protein in the Urine Samples of Alcoholics 7

According to Un Med Center, alcoholic ketoacidosis (AKA) is a clinical syndrome seen

mostly in chronic alcoholics and frequently seen in patients who binge drink.

The etiology of Alcoholic ketoacidosis stems from the patient's inability to ingest, absorb

and utilize glucose from their diet. The vomiting and nausea prevent the patient from keeping

foodstuffs in the GI tract that can cross over and provide nourishment. The alcohol further

depressed gluconeogenesis in the body and keeps blood sugar levels low. An anxiety state and

alcohol withdrawal further exacerbate the patient's ability to eat. The lack of nutrients other than

alcohol causes the creation of ketones and an elevated gap ketoacidosis in the absence of diabetes.

Protein in urine can sometimes be a sign of kidney disease, as well as other health

problems. Along with fats and sugars, proteins form the major building blocks for our bodies. The

right amount of protein is important in our diets, for growth and repair. Protein is present in the

blood; healthy kidneys should only filter tiny (trace) amounts into the urine as most protein

molecules are too large for the filters (glomeruli). It is not usual to lose protein to the urine. When

this does happen it is known as ‘Proteinuria’. Several proteins can be found in the urine, but the

most relevant to kidney disease is albumin. Protein in the urine is not usually obvious, but can be

detected by a simple dip- stick test, or sometimes by more sensitive lab tests. The presence of

protein in the urine can act as a warning signal that not all is well with the kidneys.

Chronic alcohol consumption influences protein synthesis in various organs of the human

body. Specifically , the altered redox state with the generation of excess reduced NADH from

NAD+ via ethanol oxidation in the liver affects the” free energy” transfer potentially needed for

protein synthesis and urea production (D. Agarwal & H. Werner Goedde, 2012).

The upper limit of normal total urine protein excretion in healthy adults in 150 to 200

mg/day. The upper limit of normal albumin excretion is 30 mg/day. Most urinary protein consists

of Tamm-Horsfall protein, a glycoprotein that is formed on the epithelial surface of the thick
Presence of Glucose and Protein in the Urine Samples of Alcoholics 8

ascending limb of the loop of Henle and early distal convoluted tubule. Disruption of a large

amount of high-molecular-weight plasma proteins that overwhelm the limited capacity of tubular

reabsorption and cause protein to appear in the urine, resulting in glomerular proteinuria (Clarkson,

et al, 2010).

While alcohol supplies calories (a drink contains 300kJ, or 70-100kcal), these are devoid

of nutrients such as minerals, proteins, and vitamins. Alcohol can also interfere with absorption of

vitamins in the small intestine and decrease their storage in the liver with modest effects on folate

(folacin or folic acid), pyridoxine (B6), thiamine (B1), nicotinic acid (niacin B3) and vitamin A.

Chronic intake of modest doses of alcohol can have some beneficial effects. A maximum

of one to two drinks per day may decrease the risk for cardiovascular death, perhaps through an

increase in high-density lipoprotein (HDL) cholesterol or changes in clotting mechanisms. (N.

Chhabra & S. Chhabra, 2012).

Although research dealing with nutritional status in alcoholics without liver disease has

been relatively sparse and only recently performed, there is a wealth of literature concerning

malnutrition in alcoholics with liver disease spanning the last 40-50 years. Studies by Patek and

co-workers in the 1940s reported poor dietary intake (especially with reference to protein), weight

loss, polyneuritis, and other evidence for malnutrition in alcoholic cirrhotics (R.E. Tarter & D.H.

Van Thiel, 2013).

Urine protein electrophoresis (UPE) can be highly informative as to the types of proteins

that are excreted under pathogenic conditions. Under normal circumstances, very little proteins is

lost in the urine that is excreted from the body and not reabsorbed. Whereas total protein in the

plasma is normally between 6 and 8 g/dL, the urinary protein concentration usually does not exceed

10-15 mg/dL. Therefore ther can be ~800 times as much protein in plasma as in urine, Furthermore,

such spot urine protein concentrations are no more than one-third to one-fourth the maximum
Presence of Glucose and Protein in the Urine Samples of Alcoholics 9

normal concentration of cerebrospinal fluid (CSF) protein (e.g., the upper limit of the reference

interval for CSF protein is ~45 mg/dl) (Neil S. Harris, MD & William E. Winter, 2012).
Presence of Glucose and Protein in the Urine Samples of Alcoholics 10

CHAPTER III

METHODOLOGY

This chapter presents the research design and methodology of the study which includes

respondents of the study, the research instrument procedure as well as the statistical treatment.

Research Design

The correlative research design was used to investigate, determine and describe the study.

This study dealt on individuals who drink alcoholic beverages at least twice a week.

Respondents of the Study

The respondents of the study were 10 individuals who drink alcoholic beverages at least

twice a week. There are four females and six males.

Locale of the study

The study was conducted at Purok Mabuhay Brgy. Avancena, Bo.3, Koronadal City, South
Cotabato.

Research Instrument Procedure

Since the researchers used a correlative research design. They applied the following steps:

1. The researchers selected 50 alcoholic individuals randomly to secure them as respondents

of the study.

2. The urine samples from the respondents was gathered an hour before conducting the data

analysis.

3. The researchers collected the result from the experiments conducted.

4. The results were weighted, tabulated, analyzed and interpreted.


Presence of Glucose and Protein in the Urine Samples of Alcoholics 11

Statistical treatment

The researchers utilized the weighted mean, frequency and percentage distribution to

answer problems 1 and 2. Multiple regressions were used to determine the significant relationship

of the problems.
Presence of Glucose and Protein in the Urine Samples of Alcoholics 12

CHAPTER IV

RESULTS AND DISCUSSION

This chapter analyses, interprets, and presents the data gathered. It also includes the
findings, conclusions and recommendations of this research. Thus, the tabular presentations
and discussions were organized based on each of the problem stated in the first chapter of
the study.
Table 1
Profile of the Participants

(n=50)

Indicators f %
Sex:
Male 40 80%
Female 10 20%
Total: 50 100%
Age:
Below 40 33 68%
Above 41 17 32%
Total: 50 100%
Frequency:
3 Times Below 46 92%

4 Times Above 4 8%
Total: 50 100%

Table 1 presents the profile of the participants using the following indicators; sex, age and

frequency of alcoholic beverages intake.

According to the table shown above, the samples are composed of 50 participants

(100%) who are considered as alcoholic individuals. They are divided according to their

sex, age and frequency of drinking. All in all there are forty males and ten females.

Participants who have the age of forty-one and above are sixteen while those who are
Presence of Glucose and Protein in the Urine Samples of Alcoholics 13

forty and below numbers thirty-four. In terms of the frequency of drinking, participants who

drink three times and below numbers forty-six while those who drink four times and above

numbers only four.

Table 2
Profile of Urine Samples

(n=50)

Glucose Albumin Bile

Indicators + % - % + % - % + % - %

Sex Male 40 80% 0 0 0 0 40 80% 0 0 40 80%

Female 10 20% 0 0 0 0 10 20% 0 0 10 20%

Total 50 100% 0 0 0 0 50 100% 0 0 50 100%

Glucose Albumin Bile

Indicators + % - % + % - % + % - %
40 and
Age 33 66% 0 0 0 0 33 66% 0 0 33 66%
below
41 and
17 34% 0 0 0 0 17 34% 0 0 17 34%
above
Total 50 100% 0 0 0 0 50 100% 0 0 50 100%

Glucose Albumin Bile

Indicators + % - % + % - % + % - %

3x a week
Frequency and below 46 92% 0 0 0 0 46 92% 0 0 46 92%

4x a week
4 8% 0 0 0 0 4 8% 0 0 8 8%
and above

Total 50 100% 0 0 0 0 50 100% 0 0 50 100%


Presence of Glucose and Protein in the Urine Samples of Alcoholics 14

Table 2 shows the profile of urine samples according to the following indicators namely:

sex, age and frequency of beverage intake.

For sex, the presence of glucose for 40 males as well as to the 10 females in a

total of one hundred percent of the participants are all positive. On the other hand, the

presence of albumin the same us to the presence of bile for 40 males and 10 females all

in all one hundred percent of the participants are all negative.

In terms of age, according to the table above, the presence of glucose for

participants that ages 41 and above as well as those who ages 40 and below are 100%

positive. While again the presence of albumin and the presence of bile for all participants

ages 41 up and 40 below are 100% negative.

For the frequency of drinking, the table shows that 100% of both participants

who drink alcohol 3 times below and 4 times above in a week are all positive with the

presence of glucose and again, 100% of the participants are negative for the presence

of albumin and bile in their urine samples.

Table 3
Summary table for Phi Correlation

x y r df tcomp t-test at Decision


5%
Presence of 0 49 0 0 Accept
Glucose Ho
Presence of 0 49 0 0 Accept
sex Albumin Ho

Presence of 0 49 0 0 Accept
Bile Ho
Presence of Glucose and Protein in the Urine Samples of Alcoholics 15

Presence of Accept
Glucose 0 49 0 0 Ho

Presence of 0 49 0 0 Accept
age
Albumin Ho

Presence of 0 49 0 0 Accept
Bile Ho

Presence of 0 49 0 0 Accept
Glucose Ho

Presence of 0 49 0 0 Accept
frequency
Albumin Ho

Presence of 0 49 0 0 Accept
Bile Ho

Table 3 shows that using the Phi Coefficient of Correlation, the result in each

indicator categories namely; sex, age and frequency is 0 therefor the decision is

to accept the null hypothesis because there is no significant relationship between

the presence of the said substances to the urine samples of the alcoholics.
Presence of Glucose and Protein in the Urine Samples of Alcoholics 16

Chapter V

FINDINGS, CONCLUSIONS AND RECOMMENDATIONS

This chapter holds the summary of the findings, conclusions, and recommendations based

on the result of the gathered data in accordance to the study.

A. Summary of Findings:

Based on the interpretation of the data gathered the following are the summarized findings:

1. Table 1 provides the profile of participants who are considered alcoholics because of the

amount of alcohol they take per week. They have a total of 50 individuals. The participants are

divided into three categories of classifications which is according to their sex, age and their

frequency of drinking alcoholic beverages.

Under the category of sex, the participants are composed of 40 males and 10 females. For the

category under age, the participants are divided into those who ages 40 and below and 41 and

above. The last category is the frequency of drinking, the participants again are divided to those

who drink 3 time below and 4 times above per week.

2. Table 2 presents the result of the experiment for the presence of glucose, albumin and bile

in the urine samples.

The result shows that 100% of the participants both male and female are positive with the

presence of glucose in their urine samples but 100% of them are negative for the presence of

albumin and bile. Similar to the category for sex, in terms of age and the frequency of drinking,

100% of the participants are positive with the presence of glucose but 100% negative with the

presence of albumin and bile in their urine samples.


Presence of Glucose and Protein in the Urine Samples of Alcoholics 17

3. Table 3 shows that using the Phi Correlation, the result came out is zero therefor there is no

significant relationship between the presence of glucose, albumin and bile to the urine samples of

the participants under the category their sex, age and frequency of drinking.

B. Conclusion

Based on the findings of the study, the researchers concluded that there is no significant

relationship between alcohol intake and the presence of glucose, albumin and the bile to the urine.

This means that the presence of the three substances in the urine are not affected by the amount of

alcohol that a person consume. Though the glucose are all positive, the researchers concluded that

due a 100% result, it may resulted from the other foods or drinks that the participants are

consuming. The statement testifies by the result on table 2 and 3 corresponding to the presence of

glucose, albumin and bile to the urine.

C. Recommendations

Based on the conclusion and result of the study, the following recommendations were drawn:

1. Though there is no significant relationship between the presence of glucose and protein to

the urine of alcoholics, those who choose to drink alcoholic beverages should do so

sensibly and in moderation—defined as the consumption of up to one drink per day for

women and up to two drinks per day for men.

2. Alcoholic beverages should not be consumed by some individuals, including those who

cannot restrict their alcohol intake, women of childbearing age who may become pregnant,

pregnant and lactating women, children and adolescents, individuals taking medications

that can interact with alcohol, and those with specific medical conditions.

3. Alcoholic beverages should be avoided by individuals engaging in activities that require

attention, skill, or coordination, such as driving or operating machinery.


Presence of Glucose and Protein in the Urine Samples of Alcoholics 18

References:

Galbicsek C. (2018)… What is Alcoholism?. South Orange Avenue, Suite 1450. 2019.

Retrieved from https://www.alcoholrehabguide.org/alcohol/

National Kidney Foundation, Inc. (2016),… What is a Urinalysis (also called a "urine test")?,

30 East 33rd Street, New York, NY 10016, 2019. Retrieved from

https://www.kidney.org/atoz/content/what-urinalysis

Kennedy M.N. (2018)… Diabetes and Alcohol. Diabetes Teaching Center at the University of

California, San Francisco. 2019. Retrieved from https://dtc.ucsf.edu/living-with-

diabetes/diet-and-nutrition/diabetes-alcohol/

Howard R. and Bokhari S. (2018)… Alcoholic Ketoacidosis (AKA). Tulane Un, Un Med Center

New Orleans. 2019. Retrieved from https://www.ncbi.nlm.nih.gov/books/NBK430922/

Hall N. (2013). Kidney Research UK. Peterborough. 2019 Retrieved from


https://www.kidneyresearchuk.org/health-information/proteinuria

Agarwal D. and Goedde H. (2012). Alcohol Metabolism, Alcohol Intolerance, and Alcoholism:
Biochemical and Pharmacogenetic Approaches. Springer Science & Business Media.

Clarkson M., Brenner B. and Magee C. (2010) Pocket Companion to Brenner and Rector's The

Kidney E-Book. Publisher Elsevier Health Sciences.

Chhabra N. and Chhabra S. (2012) A Case Oriented Approach Towards Biochemistry. JP

Medical Ltd.

Tarter R.E. and Thiel D.H. (2013. Alcohol and the Brain: Chronic Effects. Springer Science &

Business Media.

Harris N. and Winter W. (2012) Multiple Myeloma and Related Serum Protein Disorders:

An Electrophoretic Guide. Demos Medical Publishing.


Presence of Glucose and Protein in the Urine Samples of Alcoholics 19

APPENDIX

RAW DATA

Frequency
Respondents Age Sex Glucose Albumin Bile
(per week)

A
A1 46 M 3x + - -
A2 30 F 3x + - -
A3 19 M 3x + - -
A4 40 M 3x + - -
A5 46 F 3x + - -
A6 31 M 4x + - -
A7 26 F 3x + - -
A8 37 M 3x + - -
A9 21 F 3x + - -
A10 43 M 3x + - -
B
B1 23 F 2x + - -
B2 49 M 3x + - -
B3 25 M 2x + - -
B4 22 M 2x + - -
B5 27 M 2x + - -
B6 45 M 2x + - -
B7 43 F 3x + - -
B8 28 M 3x + - -
B9 46 M 2x + - -
B10 22 M 3x + - -
C
C1 42 M 3x + - -
C2 44 M 2x + - -
C3 23 M 2x + - -
C4 24 M 2x + - -
C5 25 M 2x + - -
C6 30 M 2x + - -
C7 46 M 3x + - -
C8 25 M 2x + - -
C9 42 M 2x + - -
C10 39 M 2x + - -
Presence of Glucose and Protein in the Urine Samples of Alcoholics 20

D
D1 40 M 3x + - -
D2 46 M 3x + - -
D3 41 M 3x + - -
D4 27 M 3x + - -
D5 25 M 3x + - -
D6 27 M 3x + - -
D7 22 M 2x + - -
D8 22 F 3x + - -
D9 22 F 3x + - -
D10 25 F 3x + - -
E
E1 56 M Daily + - -
E2 32 M Daily + - -
E3 43 M 2x + - -
E4 67 M 2x + - -
E5 29 M 2x + - -
E6 37 F 1x + - -
E7 52 M 2x + - -
E8 42 M Daily + - -
E9 33 M 1x + - -
E10 30 M 2x + - -
Presence of Glucose and Protein in the Urine Samples of Alcoholics 21

COMPUTATIONS

PHI COEFFICIENT OF CORRELATION

SEX

Glucose

AGE Positive Negative Total


t-test
40 and
40 (a) 0 (b) 40 (e)
below 𝑁−2
41 and 𝑡 = 𝑟√
10 (c) 0 (d) 10 (f) 1 − 𝑟²
above
total 50 (g) 0 (h) 50
50 − 2
𝑡 = 0√
1 − 0²
Θ = ad-bc
48
√𝑒𝑓𝑔ℎ 𝑡 = 0√
1
Θ = (40)(0)-(0)(10)
𝑡 = 0(6.92820323)
√(40)(10)(50)(0)
t=0
Θ= 0
Decision: Accept HΘ
√𝟎

Θ=0

Albumin

AGE Positive Negative Total


t-test
40 and
0 (a) 40 (b) 40 (e)
below
𝑁−2
41 and 𝑡 = 𝑟√
0 (c) 10 (d) 10 (f) 1 − 𝑟²
above
total 0 (g) 50 (h) 50
50 − 2
𝑡 = 0√
1 − 0²
Θ = ad-bc
√𝑒𝑓𝑔ℎ 48
𝑡 = 0√
1
Θ = (0)(10)-(40)(10)
√(40)(10)(0)(50) 𝑡 = 0(6.92820323)

Θ= 0 t=0

√𝟎 Decision: Accept HΘ

Θ=0
Presence of Glucose and Protein in the Urine Samples of Alcoholics 22

Bile

AGE Positive Negative Total t-test


40 and
0 (a) 40 (b) 40 (e) 𝑁−2
below 𝑡 = 𝑟√
41 and 1 − 𝑟²
0 (c) 10 (d) 10 (f)
above
total 0 (g) 50 (h) 50 50 − 2
𝑡 = 0√
1 − 0²

Θ = ad-bc 48
𝑡 = 0√
√𝑒𝑓𝑔ℎ 1

Θ = (0)(10)-(40)(10) 𝑡 = 0(6.92820323)
√(40)(10)(0)(50) t=0

Θ= 0 Decision: Accept HΘ
√𝟎

Θ=0

AGE

Glucose

SEX Positive Negative Total t-test

Male 33 (a) 0 (b) 33 (e) 𝑁−2


Female 17 (c) 0 (d) 17 (f) 𝑡 = 𝑟√
1 − 𝑟²
total 50 (g) 0 (h) 50
50 − 2
𝑡 = 0√
1 − 0²
Θ = ad-bc
√𝑒𝑓𝑔ℎ
48
𝑡 = 0√
Θ = (33)(0)-(0)(17) 1

√(33)(17)(50)(0) 𝑡 = 0(6.92820323)

Θ= 0 t=0
√𝟎 Decision: Accept HΘ
Θ=0
Presence of Glucose and Protein in the Urine Samples of Alcoholics 23

Albumin

SEX Positive Negative Total t-test


Male 0 (a) 33 (b) 33 (e)
Female 0 (c) 17 (d) 17 (f) 𝑁−2
𝑡 = 𝑟√
0 (g) 50 (h) 50 1 − 𝑟²
total

50 − 2
𝑡 = 0√
1 − 0²
Θ = ad-bc
√𝑒𝑓𝑔ℎ 48
𝑡 = 0√
1
Θ = (0)(17)-(33)(0)
√(33)(17)(0)(50) 𝑡 = 0(6.92820323)

Θ= 0 t=0

√𝟎 Decision: Accept HΘ

Θ=0

Bile

SEX Positive Negative Total


t-test
Male 0 (a) 33 (b) 33 (e)
Female 0 (c) 17 (d) 17 (f) 𝑁−2
total 0 (g) 50 (h) 50 𝑡 = 𝑟√
1 − 𝑟²

Θ = ad-bc 50 − 2
𝑡 = 0√
1 − 0²
√𝑒𝑓𝑔ℎ

Θ = (0)(17)-(33)(0) 𝑡 = 0√
48
1
√(33)(17)(0)(50)
𝑡 = 0(6.92820323)
Θ= 0
√𝟎 t=0

Θ=0 Decision: Accept HΘ


Presence of Glucose and Protein in the Urine Samples of Alcoholics 24

FREQUENCY (per week)

Glucose

FREQUENCY Positive Negative Total


t-test
3x a week
46 (a) 0 (b) 40 (e)
and below
𝑁−2
4x a week 𝑡 = 𝑟√
4 (c) 0 (d) 10 (f) 1 − 𝑟²
and above
total 50 (g) 0 (h) 50
50 − 2
𝑡 = 0√
1 − 0²

Θ = ad-bc 48
𝑡 = 0√
√𝑒𝑓𝑔ℎ 1
Θ = (46)(0)-(0)(4)
𝑡 = 0(6.92820323)
√(46)(4)(50)(0)
t=0
Θ= 0
√𝟎 Decision: Accept HΘ

Θ=0

Albumin

FREQUENCY Positive Negative Total


t-test
3x a week
0 (a) 46 (b) 46 (e)
and below 𝑁−2
4x a week 𝑡 = 𝑟√
0 (c) 4 (d) 4 (f) 1 − 𝑟²
and above
total 0 (g) 50 (h) 50 50 − 2
𝑡 = 0√
1 − 0²
Θ = ad-bc
√𝑒𝑓𝑔ℎ 48
𝑡 = 0√
1
Θ = (0)(4)-(46)(0)
𝑡 = 0(6.92820323)
√(46)(4)(0)(50)
t=0
Θ= 0
√𝟎 Decision: Accept HΘ

Θ=0

Bile
Presence of Glucose and Protein in the Urine Samples of Alcoholics 25

FREQUENCY Positive Negative Total


3x a week t-test
0 (a) 46 (b) 46 (e)
and below
4x a week 𝑁−2
0 (c) 4 (d) 4 (f) 𝑡 = 𝑟√
and above 1 − 𝑟²
total 0 (g) 50 (h) 50
50 − 2
𝑡 = 0√
1 − 0²
Θ = ad-bc
√𝑒𝑓𝑔ℎ 48
𝑡 = 0√
1
Θ = (0)(4)-(46)(0)
√(46)(4)(0)(50) 𝑡 = 0(6.92820323)

t=0
Θ= 0
√𝟎 Decision: Accept HΘ

Θ=0

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