2 (P dan nP).

exp 09/21/18-20:47:12 1/8

< Experiment title >

Name :

User :

Note :

Date : 09/21/18 Time : 20:47:12

< Protocol parameters >

Experiment file path : 2 (P dan nP).exp

Measurement type : End point

Measurement mode : Continuous

Main_1 filter(nm) : 600

Starting method : Immediate

Need shake : Yes

Shake speed : Low

Shake time(sec.) : 3

Need incubate : No

Need quantitative : Yes

Quant. method : Curve on plate

Quant. standards number : 8

Quant. replicates number : 2

Curve fit method : 4 param logistic

Need Cutoff : No

Need ratio/inhibition : No

< Plate layout >

1 2 3 4 5 6 7 8 9 10 11 12

A STD08-1 STD08-2 SAM01-1 SAM01-2 SAM09-1 SAM09-2 SAM17-1 SAM17-2 SAM25-1 SAM25-2 SAM33-1 SAM33-2

B STD07-1 STD07-2 SAM02-1 SAM02-2 SAM10-1 SAM10-2 SAM18-1 SAM18-2 SAM26-1 SAM26-2 SAM34-1 SAM34-2

C STD06-1 STD06-2 SAM03-1 SAM03-2 SAM11-1 SAM11-2 SAM19-1 SAM19-2 SAM27-1 SAM27-2 SAM35-1 SAM35-2

D STD05-1 STD05-2 SAM04-1 SAM04-2 SAM12-1 SAM12-2 SAM20-1 SAM20-2 SAM28-1 SAM28-2 SAM36-1 SAM36-2

E STD04-1 STD04-2 SAM05-1 SAM05-2 SAM13-1 SAM13-2 SAM21-1 SAM21-2 SAM29-1 SAM29-2 SAM37-1 SAM37-2

F STD03-1 STD03-2 SAM06-1 SAM06-2 SAM14-1 SAM14-2 SAM22-1 SAM22-2 SAM30-1 SAM30-2 SAM38-1 SAM38-2

G STD02-1 STD02-2 SAM07-1 SAM07-2 SAM15-1 SAM15-2 SAM23-1 SAM23-2 SAM31-1 SAM31-2 SAM39-1 SAM39-2

H STD01-1 STD01-2 SAM08-1 SAM08-2 SAM16-1 SAM16-2 SAM24-1 SAM24-2 SAM32-1 SAM32-2 SAM40-1 SAM40-2

< Raw abs. matrix >

P1 1 2 3 4 5 6 7 8 9 10 11 12

A 1.050 1.123 0.762 0.770 0.744 0.760 1.098 1.149 0.844 0.925 0.744 0.902

B 1.068 1.118 0.752 0.761 0.798 0.768 0.989 0.930 0.791 0.912 0.527 0.518

C 1.086 1.010 0.773 0.280 0.667 0.614 1.172 0.822 0.830 1.352 1.448 0.618
 2 (P dan nP).exp 09/21/18-20:47:12 2/8

D 1.041 0.983 0.631 0.616 0.850 0.791 0.851 0.756 0.911 0.886 0.455 0.494

E 0.835 0.832 0.850 0.896 0.571 0.528 0.693 0.672 0.852 0.815 0.810 0.733

F 0.583 0.565 0.843 0.802 0.979 0.923 0.528 0.570 1.956 0.992 1.549 0.903

G 0.443 0.414 0.646 0.655 0.738 1.041 0.915 0.800 0.821 0.719 1.204 0.633

H 0.285 0.303 0.852 0.790 0.820 1.330 1.267 0.887 0.856 1.084 0.665 0.746
 2 (P dan nP).exp 09/21/18-20:47:12 3/8

< Calibration >

Calib. curve

Name Meas. Conc. Fit type : 4 param logistic

C01 STD01 0.285 Meas. scale : linear

0.303 Conc. scale : linear

0.294 25.000

C02 STD02 0.443

0.414 Abs.

0.429 125.000 2

C03 STD03 0.583

0.565

0.574 250.000

C04 STD04 0.835

0.832

0.834 500.000

C05 STD05 1.041

0.983

1.012 750.000

C06 STD06 1.086 0

1.010 0 2100

1.048 1000.000 Formula : Conc.

C07 STD07 1.068 Abs = (a0-a3)/(1+power(C/a2,a1))+a3

1.118 a0 : 0.3042

1.093 1500.000 a1 : 1.96

C08 STD08 1.050 a2 : 351.7

1.123 a3 : 1.134

1.087 2000.000 R2 : 0.9956

Residuals table

C(set) Abs. C(cal) Residual

C01 25.000 0.294 25.000 0.000

C02 125.000 0.429 144.602 19.602

C03 250.000 0.574 242.213 -7.787

C04 500.000 0.834 468.700 -31.300

C05 750.000 1.012 862.024 112.024

C06 1000.000 1.048 1056.605 56.605

C07 1500.000 1.093 1587.609 87.609

C08 2000.000 1.087 1458.593 -541.407

 2 (P dan nP).exp 09/21/18-20:47:12 4/8

Conc. matrix

1 2 3 4 5 6 7 8 9 10 11 12

A 1458.593 1458.593 394.770 394.770 381.285 381.285 3153.070 3153.070 540.041 540.041 456.482 456.482

B 1587.609 1587.609 385.081 385.081 411.941 411.941 689.265 689.265 492.010 492.010 207.522 207.522

C 1056.605 1056.605 210.160 210.160 288.727 288.727 803.707 803.707 1547.595 1547.595 963.420 963.420

D 862.024 862.024 276.360 276.360 452.909 452.909 433.414 433.414 562.963 562.963 175.851 175.851

E 468.700 468.700 523.170 523.170 225.406 225.406 320.845 320.845 468.700 468.700 399.726 399.726

F 242.213 242.213 455.286 455.286 669.518 669.518 225.406 225.406 0.000 0.000 0.000 0.000

G 144.602 144.602 296.157 296.157 548.030 548.030 500.195 500.195 398.729 398.729 599.013 599.013

H 25.000 25.000 454.095 454.095 1303.691 1303.691 1328.541 1328.541 718.547 718.547 339.598 339.598

< Data list >

SAMPLES

Name Well Replicate Abs. SD CV% Conc. Unit Cutoff R/I(%)

SAM01 A3 1 0.762 ........ ........ ........ ........ N/A ........

SAM01 A4 2 0.770 ........ ........ ........ ........ N/A ........

SAM01_avg ........ ........ 0.766 0.004 0.52 394.770 ug/mL N/A N/A

SAM02 B3 1 0.752 ........ ........ ........ ........ N/A ........

SAM02 B4 2 0.761 ........ ........ ........ ........ N/A ........

SAM02_avg ........ ........ 0.757 0.005 0.59 385.081 ug/mL N/A N/A

SAM03 C3 1 0.773 ........ ........ ........ ........ N/A ........

SAM03 C4 2 0.280 ........ ........ ........ ........ N/A ........

SAM03_avg ........ ........ 0.527 0.247 46.82 210.160 ug/mL N/A N/A

SAM04 D3 1 0.631 ........ ........ ........ ........ N/A ........

SAM04 D4 2 0.616 ........ ........ ........ ........ N/A ........

SAM04_avg ........ ........ 0.624 0.007 1.2 276.360 ug/mL N/A N/A

SAM05 E3 1 0.850 ........ ........ ........ ........ N/A ........

SAM05 E4 2 0.896 ........ ........ ........ ........ N/A ........

SAM05_avg ........ ........ 0.873 0.023 2.63 523.170 ug/mL N/A N/A

SAM06 F3 1 0.843 ........ ........ ........ ........ N/A ........

SAM06 F4 2 0.802 ........ ........ ........ ........ N/A ........

SAM06_avg ........ ........ 0.823 0.021 2.49 455.286 ug/mL N/A N/A

SAM07 G3 1 0.646 ........ ........ ........ ........ N/A ........

SAM07 G4 2 0.655 ........ ........ ........ ........ N/A ........

SAM07_avg ........ ........ 0.651 0.004 0.69 296.157 ug/mL N/A N/A

SAM08 H3 1 0.852 ........ ........ ........ ........ N/A ........

SAM08 H4 2 0.790 ........ ........ ........ ........ N/A ........

SAM08_avg ........ ........ 0.821 0.031 3.78 454.095 ug/mL N/A N/A

SAM09 A5 1 0.744 ........ ........ ........ ........ N/A ........

SAM09 A6 2 0.760 ........ ........ ........ ........ N/A ........

SAM09_avg ........ ........ 0.752 0.008 1.06 381.285 ug/mL N/A N/A
 2 (P dan nP).exp 09/21/18-20:47:12 5/8

SAM10 B5 1 0.798 ........ ........ ........ ........ N/A ........

SAM10 B6 2 0.768 ........ ........ ........ ........ N/A ........

SAM10_avg ........ ........ 0.783 0.015 1.92 411.941 ug/mL N/A N/A

SAM11 C5 1 0.667 ........ ........ ........ ........ N/A ........

SAM11 C6 2 0.614 ........ ........ ........ ........ N/A ........

SAM11_avg ........ ........ 0.641 0.026 4.14 288.727 ug/mL N/A N/A

SAM12 D5 1 0.850 ........ ........ ........ ........ N/A ........

SAM12 D6 2 0.791 ........ ........ ........ ........ N/A ........

SAM12_avg ........ ........ 0.821 0.030 3.6 452.909 ug/mL N/A N/A

SAM13 E5 1 0.571 ........ ........ ........ ........ N/A ........

SAM13 E6 2 0.528 ........ ........ ........ ........ N/A ........

SAM13_avg ........ ........ 0.550 0.021 3.91 225.406 ug/mL N/A N/A

SAM14 F5 1 0.979 ........ ........ ........ ........ N/A ........

SAM14 F6 2 0.923 ........ ........ ........ ........ N/A ........

SAM14_avg ........ ........ 0.951 0.028 2.94 669.518 ug/mL N/A N/A

SAM15 G5 1 0.738 ........ ........ ........ ........ N/A ........

SAM15 G6 2 1.041 ........ ........ ........ ........ N/A ........

SAM15_avg ........ ........ 0.890 0.152 17.03 548.030 ug/mL N/A N/A

SAM16 H5 1 0.820 ........ ........ ........ ........ N/A ........

SAM16 H6 2 1.330 ........ ........ ........ ........ N/A ........

SAM16_avg ........ ........ 1.075 0.255 23.72 1303.691 ug/mL N/A N/A

SAM17 A7 1 1.098 ........ ........ ........ ........ N/A ........

SAM17 A8 2 1.149 ........ ........ ........ ........ N/A ........

SAM17_avg ........ ........ 1.124 0.026 2.27 3153.070 ug/mL N/A N/A

SAM18 B7 1 0.989 ........ ........ ........ ........ N/A ........

SAM18 B8 2 0.930 ........ ........ ........ ........ N/A ........

SAM18_avg ........ ........ 0.960 0.030 3.07 689.265 ug/mL N/A N/A

SAM19 C7 1 1.172 ........ ........ ........ ........ N/A ........

SAM19 C8 2 0.822 ........ ........ ........ ........ N/A ........

SAM19_avg ........ ........ 0.997 0.175 17.55 803.707 ug/mL N/A N/A

SAM20 D7 1 0.851 ........ ........ ........ ........ N/A ........

SAM20 D8 2 0.756 ........ ........ ........ ........ N/A ........

SAM20_avg ........ ........ 0.804 0.048 5.91 433.414 ug/mL N/A N/A

SAM21 E7 1 0.693 ........ ........ ........ ........ N/A ........

SAM21 E8 2 0.672 ........ ........ ........ ........ N/A ........

SAM21_avg ........ ........ 0.683 0.011 1.54 320.845 ug/mL N/A N/A

SAM22 F7 1 0.528 ........ ........ ........ ........ N/A ........

SAM22 F8 2 0.570 ........ ........ ........ ........ N/A ........

SAM22_avg ........ ........ 0.549 0.021 3.83 225.406 ug/mL N/A N/A

SAM23 G7 1 0.915 ........ ........ ........ ........ N/A ........

SAM23 G8 2 0.800 ........ ........ ........ ........ N/A ........

SAM23_avg ........ ........ 0.858 0.058 6.71 500.195 ug/mL N/A N/A

SAM24 H7 1 1.267 ........ ........ ........ ........ N/A ........

SAM24 H8 2 0.887 ........ ........ ........ ........ N/A ........

 2 (P dan nP).exp 09/21/18-20:47:12 6/8

SAM24_avg ........ ........ 1.077 0.190 17.64 1328.541 ug/mL N/A N/A

SAM25 A9 1 0.844 ........ ........ ........ ........ N/A ........

SAM25 A10 2 0.925 ........ ........ ........ ........ N/A ........

SAM25_avg ........ ........ 0.885 0.041 4.58 540.041 ug/mL N/A N/A

SAM26 B9 1 0.791 ........ ........ ........ ........ N/A ........

SAM26 B10 2 0.912 ........ ........ ........ ........ N/A ........

SAM26_avg ........ ........ 0.852 0.061 7.11 492.010 ug/mL N/A N/A

SAM27 C9 1 0.830 ........ ........ ........ ........ N/A ........

SAM27 C10 2 1.352 ........ ........ ........ ........ N/A ........

SAM27_avg ........ ........ 1.091 0.261 23.92 1547.595 ug/mL N/A N/A

SAM28 D9 1 0.911 ........ ........ ........ ........ N/A ........

SAM28 D10 2 0.886 ........ ........ ........ ........ N/A ........

SAM28_avg ........ ........ 0.899 0.013 1.39 562.963 ug/mL N/A N/A

SAM29 E9 1 0.852 ........ ........ ........ ........ N/A ........

SAM29 E10 2 0.815 ........ ........ ........ ........ N/A ........

SAM29_avg ........ ........ 0.834 0.019 2.22 468.700 ug/mL N/A N/A

SAM30 F9 1 1.956 ........ ........ ........ ........ N/A ........

SAM30 F10 2 0.992 ........ ........ ........ ........ N/A ........

SAM30_avg ........ ........ 1.474 0.482 32.7 0.000 ug/mL N/A N/A

SAM31 G9 1 0.821 ........ ........ ........ ........ N/A ........

SAM31 G10 2 0.719 ........ ........ ........ ........ N/A ........

SAM31_avg ........ ........ 0.770 0.051 6.62 398.729 ug/mL N/A N/A

SAM32 H9 1 0.856 ........ ........ ........ ........ N/A ........

SAM32 H10 2 1.084 ........ ........ ........ ........ N/A ........

SAM32_avg ........ ........ 0.970 0.114 11.75 718.547 ug/mL N/A N/A

SAM33 A11 1 0.744 ........ ........ ........ ........ N/A ........

SAM33 A12 2 0.902 ........ ........ ........ ........ N/A ........

SAM33_avg ........ ........ 0.823 0.079 9.6 456.482 ug/mL N/A N/A

SAM34 B11 1 0.527 ........ ........ ........ ........ N/A ........

SAM34 B12 2 0.518 ........ ........ ........ ........ N/A ........

SAM34_avg ........ ........ 0.523 0.005 0.86 207.522 ug/mL N/A N/A

SAM35 C11 1 1.448 ........ ........ ........ ........ N/A ........

SAM35 C12 2 0.618 ........ ........ ........ ........ N/A ........

SAM35_avg ........ ........ 1.033 0.415 40.17 963.420 ug/mL N/A N/A

SAM36 D11 1 0.455 ........ ........ ........ ........ N/A ........

SAM36 D12 2 0.494 ........ ........ ........ ........ N/A ........

SAM36_avg ........ ........ 0.475 0.019 4.11 175.851 ug/mL N/A N/A

SAM37 E11 1 0.810 ........ ........ ........ ........ N/A ........

SAM37 E12 2 0.733 ........ ........ ........ ........ N/A ........

SAM37_avg ........ ........ 0.772 0.039 4.99 399.726 ug/mL N/A N/A

SAM38 F11 1 1.549 ........ ........ ........ ........ N/A ........

SAM38 F12 2 0.903 ........ ........ ........ ........ N/A ........

SAM38_avg ........ ........ 1.226 0.323 26.35 0.000 ug/mL N/A N/A

SAM39 G11 1 1.204 ........ ........ ........ ........ N/A ........

 2 (P dan nP).exp 09/21/18-20:47:12 7/8

SAM39 G12 2 0.633 ........ ........ ........ ........ N/A ........

SAM39_avg ........ ........ 0.919 0.286 31.08 599.013 ug/mL N/A N/A

SAM40 H11 1 0.665 ........ ........ ........ ........ N/A ........

SAM40 H12 2 0.746 ........ ........ ........ ........ N/A ........

SAM40_avg ........ ........ 0.706 0.040 5.74 339.598 ug/mL N/A N/A

STANDARDS

Name Well Replicate Abs. SD CV% Conc. Unit Cutoff R/I(%)

STD01 H1 1 0.285 ........ ........ ........ ........ N/A ........

STD01 H2 2 0.303 ........ ........ ........ ........ N/A ........

STD01_avg ........ ........ 0.294 0.009 3.06 25.000 ug/mL N/A N/A

STD02 G1 1 0.443 ........ ........ ........ ........ N/A ........

STD02 G2 2 0.414 ........ ........ ........ ........ N/A ........

STD02_avg ........ ........ 0.429 0.014 3.38 144.602 ug/mL N/A N/A

STD03 F1 1 0.583 ........ ........ ........ ........ N/A ........

STD03 F2 2 0.565 ........ ........ ........ ........ N/A ........

STD03_avg ........ ........ 0.574 0.009 1.57 242.213 ug/mL N/A N/A

STD04 E1 1 0.835 ........ ........ ........ ........ N/A ........

STD04 E2 2 0.832 ........ ........ ........ ........ N/A ........

STD04_avg ........ ........ 0.834 0.001 0.18 468.700 ug/mL N/A N/A

STD05 D1 1 1.041 ........ ........ ........ ........ N/A ........

STD05 D2 2 0.983 ........ ........ ........ ........ N/A ........

STD05_avg ........ ........ 1.012 0.029 2.87 862.024 ug/mL N/A N/A

STD06 C1 1 1.086 ........ ........ ........ ........ N/A ........

STD06 C2 2 1.010 ........ ........ ........ ........ N/A ........

STD06_avg ........ ........ 1.048 0.038 3.63 1056.605 ug/mL N/A N/A

STD07 B1 1 1.068 ........ ........ ........ ........ N/A ........

STD07 B2 2 1.118 ........ ........ ........ ........ N/A ........

STD07_avg ........ ........ 1.093 0.025 2.29 1587.609 ug/mL N/A N/A

STD08 A1 1 1.050 ........ ........ ........ ........ N/A ........

STD08 A2 2 1.123 ........ ........ ........ ........ N/A ........

STD08_avg ........ ........ 1.087 0.037 3.36 1458.593 ug/mL N/A N/A

< End >

2 (P dan nP).exp 09/21/18-20:47:12 8/8