PLD-951 Photometer

Installation and Maintenance Manual

Alfa Wassermann Service Website: http://groups.msn.com/AlfaWassermannBV
Alfa Wassermann Inc. http://www.alfawassermannus.com/
 INSTALLATION AND
MAINTENANCE MANUAL
English

TEUS00012-01-ING
May-2004
 TABLE OF CONTENTS
1. UNPACKING ......................................................................................... 5
1.1. UNPACKING .................................................................................................. 5
1.2. LIST OF CONTENTS ..................................................................................... 5
1.2.1. Contents of the accesories box ....................................................................... 5
1.3. PARTS IDENTIFICATION ............................................................................... 5

2. INSTALLATION .................................................................................... 8
2.1. Location ......................................................................................................... 8
2.2. Versions with printer: Inserting printer paper. ............................................ 8
2.3. Versions with sipper: Installing the tubing .................................................. 8
2.4. Versions with sipper: Installing the waste bottle. ....................................... 9
2.5. CONNECTION TO POWER SUPPLY AND STARTING............................... 10
2.5.1. Quality of the electrical supply....................................................................... 10
2.5.2. Checking the supply voltage.......................................................................... 10
2.5.3. Connection to the power supply .................................................................... 10
2.6. HANDLING, STORAGE AND RESHIPMENT .............................................. 11

3. SUMMARY OF TECHNICAL SPECIFICATIONS............................... 12

4. CARE AND MAINTENANCE OF THE PHOTOMETER ..................... 14

4.1. GENERAL STANDARDS ............................................................................. 14
4.2. MAINTENANCE OF THE SIPPER (only versions with sipper) ................. 14
4.3. CHANGING THE LAMP ............................................................................... 14
4.4. ADDING A FILTER ....................................................................................... 15
4.5 WASTE ELIMINATION................................................................................... 16

5. ACCESSORIES AND REPLACEMENT PARTS................................ 17

6. WARRANTY LIMITS ........................................................................... 18

3
1. UNPACKING

This photometer is a precision instrument, so it is necessary
to exercise special care in its installation and positioning.
It is very important to connect the instrument to a good
electrical system. It should be as exclusive as possible, and
it is absolutely imperative for it to have an earth connection.
1.2.1. Contents of the accesories box
Figure 1.2 shows the possible contents of the accessories
box, depending on the instrument version.
- All versions contain:

a) A macro cuvette (16).

1.1. UNPACKING b) An adapter for test tubes (7).
c) Two fuses of 0.630 A (8).
On receiving the instrument, check that the packing is in d) Four fuses of 1.25 A (9).
perfect condition and that the seals are intact. Open the box e) Two power cables (10).
and take out its contents with care (figure 2.5). Read this f) A dust cover for the photometer (11).
chapter carefully before installing the instrument. Do not
throw the packing material away. In case of re-shipment, - Versions providing the printer contain, in addition:
use it again to guarantee good transport (section 2.6).
g) A cover for paper (12).
h) Two rolls of thermal paper (13).
1.2. LIST OF CONTENTS
- Version providing the sipper contains, in addition:
Figure 1.1 shows the set of items that the user will find on
unpacking the apparatus. i) Two meters of silicone tubing 3 x 6 mm (14).
j) Two peristaltic tubing sets (15).
a) A photometer (1). k) A flow cuvette (16).
b) A waste bottle (2). Only in version with sipper. l) Two teflon tubing (17).
c) A user’s manual (3). m) A cylindrical silicone connector (18).
d) An accessories box (4). n) A cuvette outlet adapter (19).
e) An instructions sheet for unpacking (5). o) A 100 mL bottle with wash solution (20).
f) A release protocol sheet (100)
g) A communication cable RS 232 (42)
h) A cd-rom with the program "Photometer PC program" (43)

# Only in versions with sipper.

43 1
3

42

2#

100 5

Figure 1.1 Contents of the packing

5
Installation and maintenance manual

* Only in versions with printer.

14# 20# 19# 17# # Only in versions with sipper.
$ Only versions without printer
15# and sipper
10

16#$
7

18#

9 8
11 12*
13*

Figure 1.2 Contents of the accesories box

1.3. PARTS IDENTIFICATION

The constituent parts of the photometer are identified and numbered in figures 1.3 and 1.4 and the associated lists. Please,
note that some elements are not present in all the different versions of the photometer.

* Only in versions with printer.

25 29 22 12* # Only in versions with sipper.

13*

23*

24

21

17# 26# 27# 28*

Figure 1.3 Front view

6
 # Only in versions with sipper.

30

39

31

41

32

36
35 38# 34 37 39 40

Figure 1.4 Rear view

(MODEL/SERIES)
12. PAPER ACCESS DOOR 36. LINE VOLTAGE SELECTOR
13. THERMIC PAPER 37. SERIAL CONNECTOR RS-232-C
17. SIP TUBING 38. WASTE OUTLET
21. KEYBOARD 39. SCREWS FOR SECURING THE CASE
22. DISPLAY 40. CASE
23. PRINTER 41. BASE
24. ON/OFF INDICATOR
25. CUVETTELODGING
26. PUMP KEY
27. WASH KEY
28. PAPER KEY
29. BRAND LABEL
30. SWITCH
31. FUSES
32. POWER SOCKET
34. FAN
35. IDENTIFICATION LABEL

7
Installation and maintenance manual
2. INSTALLATION
In order to ensure an optimal instrument operation, follow
the instruction given in this chapter to install the photometer.
2.1. Location
The photometer must be situated in a dry and non-corrosive
environment. In addition, it must not be near a source of
electro-magnetic radiation (such as motors, centrifuges,
etc.), nor a source of heat, nor directly receive intense lamp
or sun light.
It must be located on a flat and spacious surface, taking
care that no object obstructs the outlet of air from the fan.
Leave at least 15 cm to the nearest wall.
In the versions providing a sipper, the waste bottle must be
placed on the same level or lower than the instrument.
2.2. Versions with printer: Inserting printer
paper.
a) Open the paper access cover (12) and put a paper roll (13)
in its lodging ensuring that the thermosensible face of the
paper is facing to the bottom of the lodging.
b) Insert the paper into the slot in the rear part of the printer
and press PAPER (28) to feed the paper until it is in the right
position.
c) Finally, place the cover.
15#
C
B
A
Figure 2.1 Installing the tubing in the cuvette
8
NOTE
The intensity of printing may be adjusted
following the procedure described in section
3.4.2.6 of user’s manual.
2.3. Versions with sipper: Installing the tubing
To install the tubing see figures 2.1 and 2.2, and the
following instructions:
a) Screw the outlet adapter (19) into the flow cuvette (16),
making certain that it is firmly secured.
b) Insert the peristalting tubing (15) by the shorter end (C)
into the cuvette outlet adapter (19).
c) Secure the adapter with the teflon tube (17) in the cuvette
inlet adapter.
d) Insert the cylindrical silicone connector (18) into the teflon
tube (17) by the end with the smaller interior diameter.
e) Place the cuvette (16) into its lodging (42) with the face
(A) of the cuvette located towards the front part of the
instrument.
f) Insert the teflon tube (17) into the guide tube (43), securing
the cylindrical silicone connector (18) to it.
g) Place the peristaltic tubing (15) into the peristaltic pump
by connecting its free end to the waste adapter (44),
ensuring that it is well inserted.
18#
18#
17#
19#
16#
# Only in versions with sipper.
 44

15#

47#

42

19#
16#

# Only in versions with sipper.

47 7 46 43# 18# 17#

Figure 2.2 Sipping circuit

2.4. Versions with sipper: Installing the waste bottle.

Figure 2.3 illustrates the installation of this bottle.

a) Take the silicone tubing (14) and cut off the piece required.
b) Insert an end of the tubing into the adapter of the waste bottle.
c) Insert the other end of the tube into the waste outlet adapter (38) located in the rear part of the apparatus.

# Only in versions with sipper.

38#

14#

Figura 2.3 Installing the waste bottle

9
Installation and maintenance manual

2.5. CONNECTION TO POWER SUPPLY In the rear part of the apparatus there is the power socket.
AND STARTING When you receive the apparatus, this will be covered with a
label (45) indicating the voltage for which it is set (figure 2.8).
Starting the photometer involves observing some aspects of Although only 115 V or 230 V are stated, they correspond
the electrical supply to which it is to be connected, as well to the tolerance margins given above. Check the voltage of
as carrying out some simple checks. your electrical supply, and if it does not correspond to what
is specified in your apparatus, proceed as follows:
2.5.1. Quality of the electrical supply
a) Change the fuses (31) in accordance with the table
It is very important to connect the instrument to a good printed on the rear part of the apparatus, reproduced further
electrical system. It should be as exclusive as possible, and on.
it is absolutely imperative for it to have an earth connection.
b) Select on the line voltage selector (36) the voltage of your
If a malfunctioning of the instrument is noticed (program electrical supply.
crashes, sporadic re-starts, etc.), check that it is not near
centrifuges or machinery containing motors or electro-
magnets which can generate strong electrical noise. In
such a case, place the photometer far from such equipment. NOMINAL FUSE SPEED
230 V 0.63 A T
Installation Category (overvoltage category) : II 115 V 1.25 A T

2.5.2. Checking the supply voltage 2.5.3. Connection to the power supply
This photometer is designed to work at the following Once the voltage selected in the apparatus corresponds to
voltages: that of the electrical supply, proceed as follows:
- 115 V (tolerances: 99-137 V) a) Remove the label (45) covering the power socket (figure
- 230 V (tolerances: 198-264 V) 2.4).
b) Check that the switch is in the off (O) position.
ATTENTION c) Connect the power cable, first to the apparatus, then to
Working beyond the tolerance limits will cause the electrical supply.
the apparatus to function incorrectly and the d) Put the switch in the on ( I ) position.
photometer may be damaged.

45 31 36 37

Figure 2.4 Connection to power supply

10
2.6. HANDLING, STORAGE AND RESHIPMENT
It must be beared in mind that this photometer is a precision
instrument so that it must be handled with attention and
special care.
If the photometer must be stored for a long time it is
recommended to clean exhaustively all the sipping circuit.
First with a washing solution and then rinse gently with
distilled water. The sipping circuit must be emptied when
Figure 2.5 Packing
11
the cleaning process is finished. Take the peristaltic tubing
out from its mounting. The photometer must be protected
from environmental aggressions, like dust, excess of humidity
or direct solar light.
If it is necessary to re-ship the photometer for any reason,
or to make a move involving the use of any transport vehicle,
it is important to use the original packing to ensure that the
apparatus does not suffer any damage. Figure 2.5 shows
you how the apparatus and its accesories must be packed.
Installation and maintenance manual

3. SUMMARY OF TECHNICAL SPECIFICATIONS

A) Optical system

Absorbance range: 0 to 2 A
Filters wheel with up to: 9
Filter wheel configuration: 340,405,505,546,600,635,670
Wavelenght error: ± 2 nm
Bandwidth: 10 ± 2 nm
Digital Resolution: 0.0001 A
Base Line Stability: max. 0.005A in 30 minutes at 405nm
Repeatability: ±0.0012 at 0.1A, 20 readings at 505nm (CV <= 1.2 %)
±0.0015 at 1.5A, 20 readings at 505nm (CV <= 0.1 %)
Accuracy: For 340, 405 and 505 nm:
± 0.005A at 0,1 A (± 5%)
± 0.020A at 1,0 A (± 2 %)
± 0.040A at 2,0 A (± 2 %)
B) Thermostatic control

Thermostatic range: 23 to 40º C

Accuracy: ± 0.5ºC
Stability: ± 0.2º C

C) Installation

Voltage: 115V (99-137V) / 230V (198-264V)

Frecuency: 50/60 Hz
Maximum apparent power: 60 VA
Dimensions: 387 x 337 x 150 mm (15.2 x 13.3 x 5.9 in)
Weight: 9.5 Kg (21 lb)

D) Cuvettes

Flow cuvette (only version with sipper)

Common cuvettes (macro, semimicro, micro)
Round test tubes (12 mm diameter x 75 mm length)

E) Sipper system (only version providing sipper)

Continuous flow system, with peristaltic pump included in the apparatus itself.
Pump operated by means of a low-voltage stepper motor
Sipping volume programmable from 100 to 4000 mL
Capacity of the flow cuvette: 18 mL
Automatic adjust of the sample volume
Automatic adjust of the sample positioning

F) Enviromental conditions

Indoor use
Altitude: < 2000m
Temperature: 15 - 35º
Maximum relative humidity: 75%
Installation Categorie (overvoltage categorie): II
Pollution degree: 2

12
G) Compliance with applicable directives and norms

Directive 98/79/CE related with In Vitro Diagnostic products

UNE EN 61326-1 “Electromagnetic equipment for measurement, control and laboratory use –ECM requirements. Part
1: General requirements”.
IEC 1000-3-2 “Electromagnetic compatibility (EMC) – Part 3: Limits –Section 2: Limits for harmonic current
emissions”.
IEC 1000-3-3 “Electromagnetic compatibility (EMC) – Part 3: Limits –Section 3: Limitation of voltage fluctuations
and flicker in low-voltage supply systems”.
CISPR 22 “Information technology equipment – Radio disturbance characteristics - Limits and methods of
measurement”.
Continuous conducted: Class A
Radiated: Class A
IEC 1000-4-2 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques – Section 2:
Electrostatic discharge immunity test”.
IEC 1000-4-3 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques – Section 3:
Radiated, radio-frequency, electromagnetic field immunity test”.
IEC 1000-4-4 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques – Section 4:
Electrical fast transient/burst immunity test”.
IEC 1000-4-5 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques – Section 5:
Surge immunity test”.
IEC 1000-4-6 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques – Section 6:
Immunity to conducted disturbances, induced by radio-frequency fields”.
IEC 1000-4-11 “Electromagnetic compatibility (EMC) – Part 4: Testing and measurement techniques – Section 11:
Voltage dips, short interruptions and voltage variations immunity tests”.
UNE-EN 61010-1 “Safety requirements for electrical equipment for measurement, control and laboratory use. Part 1 –
General requirements”.

The manufacturer reserves the right to modify any technical specification without prior notice.

13
Installation and maintenance manual
4. CARE AND MAINTENANCE OF THE PHOTOMETER
To get optimal functioning of the photometer, it is necessary
to follow some minimum standards of maintenance. This
chapter explain them and also provides instructions for
changing the lamp or inserting a new filter.
4.1. GENERAL STANDARDS
a) Never use detergents nor abrasive products for cleaning
the outside of the instrument. Use only a cloth with water
and neutral soap.
b) If a reagent or any corrosive product is spilt on the
apparatus, clean it immediately with a damp cloth and
water.
c) The cuvette holder tray is equipped with watertight joints
in order to prevent penetration of liquid into the inner part
of the instrument. If liquid is spilt into the tray, clean it with
damp paper or cloth. A drainage hole connected to the
outside by a silicone tubing is in the front left part of the
tray to facilitate drainage of poured liquid.
d) If a cuvette breaks into the cuvette holder or for any reason
liquid pours into it, the holder has a drainage hole
connected to the outside. It will, however, be necessary
to rinse and dry the inside of the cuvette holder
e) Cover the photometer with its dust cover when not in use.
4.2. MAINTENANCE OF THE SIPPER (only
versions with sipper)
) It is necessary to clean the SIPPING CIRCUIT properly
after each series of measurements and at the end of the
day.
On finishing a series of measurements, wash the sipping
circuit with abundant distilled water.
At the end of the working day, wash thoroughly with a
detergent solution such as the one provided with the
instrument. Lastly, rinse with distilled water and empty
the circuit by performing wash cycles with air.
Finally, to maximize the life of the peristaltic tubing, it is
advisable to take it out from its mounting, so that it
remains loose and without tension. On starting a new
work session, re-insert it in its place.
b) If the outside end of the sip tubing has deteriorated, you
can cut a few milimeters of the end making a perpendicu-
lar and clean cut. In this case, you must re-adjust the
POSITIONING parameter.
14
c) Replace tubing by a new one in case of deterioration.
Always use original parts.
4.3. CHANGING THE LAMP
This instrument is equipped with a halogen lamp of 6 Volts,
10 Watts, with a lifetime of about 2,000 hours. When it is
necessary to change the lamp, proceed in the following
manner (figure 4.1):
a) With the apparatus cold and switched off, remove the
power cable.
b) Remove the printer paper roll, and take the sip tubing off
its guide.
c) Remove the 2 screws (1) that are in the lower front part
of the instrument.
d) Remove the 2 screws (2) that are in the rear part of the
instrument.
e) Lift the case carefully, pull it up and incline it to the rear,
taking care that the connecting strip that joins it to the
main board is not stretched.
f) Loosen the Allen screw (3) of the lamp’s radiator.
g) Pull back the flange (2) for securing the lamp-holder.
h) Take the lamp-holder (5) out, loosen the Allen screw (6)
and take the lamp out.
i) Insert the new lamp (7), introducing the terminals until they
reach the limit. Tighten the Allen screw (6) until the lamp
is well secured. Take care not to touch its glass bulb with
your fingers; you can use the cover in which it came
packed, cutting it on the terminals’side, and pressing until
they come out.
j) Re-insert the lamp-holder in its place. Position the flange
(4) over it and tighten the Allen screw (3).
k) Put the case back and screw in the retaining screws
again.
l) Re-position the printer paper roll (see 2.4.2) and the sip
tubing (see 2.4.3).
m) The lamp does not require any adjustment. Nevertheless
it is necessary to check that it is properly centered. To do
so, go to the CHECK LAMP option of the menu OTHER
OPTIONS (section 3.7.2. of user’s manual).
4.4. ADDING A FILTER
If you have acquired a filter with a wavelength different from
those coming with the instrument, you must insert it in one
of the two positions that are vacant in the FILTERS WHEELE.
To do so, proceed as follows (figure 4.2).
e) Lift the case carefully, pull it up and incline it to the rear,
taking care that the connecting strip that
joins it to the main board is not stretched.
f) Insert the filter (3) in a vacant position by pressing it. Avoid
touching the faces of the filters with the fingers.

a) With the instrument cold and switched off, remove the g) Put the case back and screw in the retaining screws
power cable. again.

b) Remove the printer paper roll, and take the sip tubing off h) Re-position the printer paper roll (see 2.4.2) and the sip
its guide. tubing (see 2.4.3).

c) Remove the 2 screws (1) that are in the lower front part
of the instrument.
d) Remove the 2 screws (2) that are in the rear part of the
instrument.
i) Now it is necessary to incorporate the filters in the
FILTERS TABLE. To do so, switch the instrument on and
select the path: PROGRAMMING/FILTERS TABLE
(section 3.4.3 of user’s manual), and add the value of the
filter in the position where it has been inserted. It will then
be ready to be used.

A 2 B

4
3

C D
5

4
3

Figure 4.1 Changing the lamp

15
Installation and maintenance manual

4.5 WASTE ELIMINATION

The photometer is provided with a waste bottle where all

samples are stored once the measurement is done. When
you want to eliminate in a correct and secure form all the
waste products you must apply the general criterion base
on the good practices of laboratories. This criterion and the
current legislation concerning where to install the instrument
must be known by all the laboratory staff.

A 2

Figure 4.2 Adding a filter

16
5. ACCESSORIES AND REPLACEMENT PARTS
If any accessory deteriorates or is lost, or if you need consumable material such as printer paper, always use original
material. Below are listed all those parts you may need. Please contact your usual distributor and make your order by using
the codes that are also listed. This will make the work easier and will avoid errors. You can identify the parts by referring
to figure 5.1.

CODE DESCRIPTION
User Manual
AC10450 Paper Access door
AC10410 Roll of paper *
AC10451 Flow-cuvette #
AC10452 Macro cuvette $
TU10249 Teflon tubing
AC10411 Cuvette outlet adapter #
TU10412 Cylindrical silicone connector #
AC10413 Peristaltic tubing #
AC10414 Silicone tubing 3x6 (200 cm) #
AC10453 Adapter for test tubes
AC10454 Waste bottle #
Bottle 100mL washing solution #
FU10880 Fuse 0.63 A
FU10881 Fuse 1.25 A
CA10455 European mains leed
CA10456 American mains leed
AC10457 Dust cover
LA10429 Halogen lamp 6V / 10W
FI10872 Filter set 340 nm
FI10873 Filter set 405 nm
FI10875 Filter set 505 nm
FI10876 Filter set 546 nm
FI10877 Filter set 600 nm
FI10878 Filter set 635 nm
FI10879 Filter set 670 nm

Table 5.1 Accessories and replacement parts

17
Installation and maintenance manual

Manual de usuario AC10457 Ver tabla 5.1 AC10451# AC10454#

AC10414 AC10450* AC10410* AC10452$ TU10249#

Ver tabla 5.1 AC10453 TU10412# AC10411# Solución Lavado (#)

LA10429 AC10413# FU10880 FU10881

Figura 5.2 Accessories and replacement parts

6. WARRANTY LIMITS
Any misuse (fall, negligence, outside-tolerance mains conditions, inadequate environment or location conditions), as
well as any internal manipulation of the instrument by personnel not authorised by manufacturer or any use of non original
consumable and spare parts (tubes, fuses, etc.) will invalidate the guarantee.

18