OFFICIAL ABSTRACT and CERTIFICATION 2.

I/we worked
Puffer Fish (Lagocephalus inermis) Tetrodotoxin as an Insecticide or used
equipment in
a regulated
Verny Jane D. Hucalinas, Denise Danielle B. Dilag, Krizia Nicole R. Dequilla research
Doña Montserrat Lopez Memorial High school , Rizal Street, Silay City, Neg. Occ., Philippines institution
Yes
Tetrodotoxin, a natural powder toxin which is 1 250 times No or
deadlier than cyanide, is found in puffer fish also known industrial
as globefish or swellfish. Puffer fish has long been setting:
praised in Japan as the most delicious of all fishes but
also feared because it has caused fatal poisoning. Because 3. This project is
the property of this component, the researchers opted to
a continuation
investigate the possibility of extracting the toxin and
of previous
tapping it as insecticide by determining the best
research.
concentration through the length of time of effectivity.
The subject used in the study is the cockroach, a nocturnal Yes
pest which is a potent germ carrier. The toxin found in No
organs of puffer fish like the liver may be extracted by
mashing the liver, then boiling it until liver dissolved in 4. My display
water. To further remove other particles, the mixture was board includes
passed through a filter paper. The liquid was again boiled non-published
and made to evaporate, leaving a white powdery residue. To photographs/v
determine the proportion of components, the volume of isual Yes
residue was held constant with the volume of water No
manipulated then added to a control mass of cooked rice fed depictions
upon by a control number of cockroaches, the effectivity of humans
was measured in terms of length of time the cockroaches (other than
were killed it was found out that 100% of the subjects died myself):
regardless of the ratio the components. However, that
length of time of effectivity varies with each proportion.
The purpose of this study is to eliminate cockroaches. Our
goal is to produce an insecticide out of tetrodotoxin taken
from the puffer fish that can kill pests, specifically,
cockroaches. The researchers provided an aquarium which has
five cubicles. In each cubicle, there were ten cockroaches
settled. There are different concentrations placed in each
ten cockroaches. As a result, the powdery residue has
killed several cockroaches in terms of the relationship
between the volume of the liquid component and the
mortality rate of cockroaches. Therefore, the lower the
liquid component, the faster the cockroaches died. We
conclude that our product is effective and less expensive
1. As a part of this research project, the student directly handled, manipulated, or interacted
with (check ALL that apply):
human participants potentially hazardous biological agents vertebrate
animals microorganisms rDNA tissue
Category Pick one only — mark an “X” in box at right
5. This abstract describes only procedures performed by me/us, Yes No
reflects my/our own independent research, and represents
one year’s work only

Animal Sciences 
6. I/we hereby certify that the abstract and responses to the
Behavioral & Social Sciences 
Yes No above statements are
correct and properly reflect my/our own
Biochemistry 
work.
Biomedical & Health
Sciences Biomedical  This stamp or embossed seal attests that this project is in
compliance with all federal and state laws and regulations and that
Engineering 
all appropriate reviews and approvals have been obtained including
Cellular & Molecular the final clearance by the Scientific Review Committee.
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