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We are pleased to introduce the 2009 - 2010 Lonza Cell Discovery Research Products Catalog. Inside, you will find all of
your known and trusted Lonza research products and services, as well as many new offerings designed to advance and
accellerate your research:
■ Clonetics® Primary Cells and Media - over 100 authenticated primary cell types and
optimized media, ready to use today
■ Poietics® Stem Cells and Media – authenticated adult stem cells available with
differentiation media
■ BioWhittaker® Media and Sera – classical sera and media, plus an extensive array of
serum free media including protein-free, chemically defined and non-animal
derived formulas
Look for Lonza Molecular Biology Products in the 2009 - 2010 Molecular Biology Research Products Catalog and
Sourcebook for Electrophoresis. The Molecular Biology catalog features our known and trusted products, such as
SeaKem®, NuSieve® and MetaPhor® Agarose; The FlashGel® System; and PAGEr® Precast Protein Gels, as well as our new
Sourcebook for Electrophoresis, featuring protocols and support tools for your most critical molecular biology techniques.
Request a copy of this catalog at www.lonza.com/research.
Purchase any of our products directly from Lonza by calling us at the number provided on the back of this catalog,
or use our e-commerce capabilities at www.lonza.com/research.
Should you require technical information, please contact our Scientific Support Team at the phone number provided on the
back of this catalog, or at scientific.support.eu@lonza.com.
Lonza is committed to providing the highest quality products and services, and to helping researchers worldwide
advance and accellerate life science research. For all of your cell discovery and molecular biology needs, turn to Lonza. We
appreciate your continued support and look forward to serving you.
Table of Contents
Customer Service iii Poietics® Stem Cells and Media
Terms and Conditions v Introduction 81
Trademarks vii Donor Programs 82
E-commerce inside back cover Bone Marrow and Mononuclear Cells 83
Hematopoietic Progenitor Cells and Media 86
Chapter 1 – Clonetics® and Poietics® Mesenchymal Stem Cells and Media 90
Primary Cells and Media Preadipocyte Cells and Media 91
Osteoclast Precursor Cells and Media 92
Clonetics® & Poietics® Quick Reference Guide 3 Immune System Cells and Media 93
Neural Progenitor Cells Media 97
Clonetics® Primary Cells and Media Custom Cell Isolation Services 98
Introduction 7
Clonetics® Conditionally Immortalized Cell Lines 8 Cell Biology Technical Information
Clonetics® Cell Models 9 Clonetics® Frequently Asked Questions 99
Clonetics® Technical Information 101
Hepatocytes and Media Clonetics® Media 111
Human Hepatocytes & Media 14 Poietics® Frequently Asked Questions 121
Poietics® Technical Information 122
Rat Hepatocytes & Media 17
Poietics® Media 123
Human Cells and Media
Chapter 2 – Amaxa® Nucleofection® and Transfection
Airway Epithelial Cells and Media 18
Introduction 127
Bladder Cells and Media 21 Amaxa® Nucleofector® Technology 128
Endothelial Cells and Media 23 Nucleofector® II Device 132
Fibroblast Cells and Media 36 96-well Shuttle® Device 133
Epidermal Keratinocyte Cells and Media 40 Nucleofector® Extended Guarantee and
Epidermal Melanocyte Cells and Media 45 Service Contracts 134
Mammary Epithelial Cells and Media 47
96-well Shuttle® Automation Package 135
Neural Cells and Media 49
Nucleofector® Kits for Primary Cells 138
Prostate Cells and Media 51
Renal Cells and Media 54 Nucleofector® Kits for Cell Lines 183
Cell Line Nucleofector® Kits 185
Skeletal Cells and Media 58
Cell Line Optimization Nucleofector® Kits 186
Skeletal Muscle Cells and Media 61
cGMP Cell Line Nucleofector® Kits 188
Smooth Muscle Cells and Media 64
Basic Parasite Nucleofector® Kits 188
Animal Cells and Media FAQs on Nucleofection® 189
HiFect® Transfection Reagent 191
Introduction 69 Vectors 192
Endothelial Cells and Media 69
Antibiotics 198
Rat Cardiac Myocytes and Media 72
siRNA Test Kit 200
Primary Neural Cells and Media 73
Peptide Transfection Control 201
Primary Neuron Growth Media 77
Cell Culture Reagents 78 Transport™ Protein Delivery Reagent 202
Custom Cell Services 79 Table of Contents continues on next page
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Table of Contents
Continued
Table of Contents
PrimeFect™ DNA & siRNA Transfection Reagent 203 Cell Culture Technical Information
Cell Biology Technical Information 101 Adaptation of Cell Cultures to Serum-free Medium 298
Protocols for Weaning Cell Cultures 299
Chapter 3 – Arrays
Cryopreservation and Reconstitution 300
Introduction 209 Determination of Cell Numbers 301
GeneSieve™ Query 210 Powdered Medium Preparation 303
Global Pattern Recognition™ Analysis Tool 211 Subculturing Procedures for Mammalian Cells 304
StellARray™ qPCR Arrays 211 Media FAQs 306
Sera FAQs 307
Chapter 4 – BioAssays
Cell Culture Reagents FAQs 308
Introduction 219
Formulations for BioWhittaker® Classical Media 309
BioAssay Kits 220
Cell Analysis Reagents 242 Chapter 6 – Endotoxin Detection
G Protein-Coupled Receptors 245
Introduction 325
BioAssay Services 246
Gel Clot LAL Assays 326
BioAssay FAQs 247
Endpoint Chromogenic LAL Assay 328
Chapter 5 – Cell Culture Fluorescent Assay 329
LAL Accessory Products 330
Classical Media
Introduction 251 Chapter 7 – Flexible Bioprocess Containers
BioWhittaker® Classical Media 253 Introduction 333
Platinum UltraPAK™ Bioprocess Containers 336
Specialty Media
Bioprocessing Tank Liners 337
Introduction 261
General Purpose Serum-free Media 262 ProEVA PAK™ Bioprocess Containers 338
X-VIVO™ Chemically Defined, Serum-free
Hematopoietic Cell Media 265
Chapter 8 – Lonza Custom Manufacturing
CHO Media 266 Introduction 340
NAO Freezing Medium 269 Lonza Custom Service Contacts 341
Renal Media 269 Transfection Services 342
Specialty Media - IVF 272
Specialty Media - Cytogenetics 273 Chapter 9 – Distributors and Indices
Hybridoma Media 274 Numerical Index 343
Insect-XPRESS™ Protein-free Insect Cell Medium 278 Alphabetical Index 358
ProNS0™ Chemically Defined, Protein-free Media 279
Cell Culture Reagents
Introduction 281
Balanced Salt Solutions 282
Reagents 283
Antibiotics and Antimycotics 285
Buffers and Buffered Saline 286
Animal and Human Sera
Introduction 289
Fetal Bovine Sera 294
Equine Sera 294
Bovine Sera 295
Human Sera 295
Fetal Bovine Sera
– Available Outside the USA and Canada 296
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Customer Service
U.S. and Canada
Customer Service
Ordering Information Scientific Support
To place an order, please use any of the following convenient Providing world-class technical support for our products
options: is a top priority. Our Scientific Support Representatives rely
Phone on years of laboratory experience to assist with product
Our Customer Service Representatives are ready to assist selection and help you maximize product performance.
you with ordering Monday through Friday, from 8 a.m. to – In the U.S., call us at (800) 521-0390, Monday
6 p.m. Eastern Standard Time (EST). Call from anywhere in through Friday, between 8 a.m. and 5:30 p.m. EST
the U.S. and Canada at: – Outside the U.S., call (207) 594-3400 for Molecular
Toll Free: (800) 638-8174 Biology Products or (301) 898-7025 for all others
Phone: (301) 898-7025
– You may also reach us by e-mail at
Fax scientific.support@lonza.com
(301) 845-8338
– Full product information, instructions, and MSDS are
Mail also available at www.lonza.com/research
Lonza Walkersville, Inc.
Customer Service Department
8830 Biggs Ford Road
Walkersville, MD 21793 USA
Please include all of the following information on all orders:
– Purchase order number
– Shipping address and billing address
– Contact name and telephone number
– Name and department of end user
– Quote or reserve lot information
– Item number, size, quantity, and name of products
ordered
Online Ordering
Ordering for all of our products is available, 24 hours/day,
7 days per week. Our online ordering system is secure,
fast, and convenient. Registered users enjoy the ability
to see their contracted prices, track orders, and manage
their account. Go to www.lonza.com/research for more
information.
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Customer Service
International
Customer Service
Ordering Information Please include all of the following information on all orders:
– Purchase order number
To place an order, please use any of the following convenient
– Shipping address and billing address
options:
– Contact name and telephone number
Phone
Our Customer Service Representatives are ready to assist – Name and department of end user
you with ordering Monday through Friday. – Quote or reserve lot information
International Offices – Item number, size, quantity, and name of products
Australia 61 3 9550 0883 ordered
Austria 0800 201 538
Online Ordering
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Online ordering for all of our products is available, 24
Brazil 55 11 2069 88 00
hours/day, 7 days per week. Our online ordering system
Denmark 45 43 56 74 00
is secure, fast, and convenient. Registered users enjoy the
France 0800 91 19 81
ability to see their contracted prices, track orders, and
Germany 0800 182 52 87
manage their account. Go to www.lonza.com/research for
India 91 22 6697 2883
more information.
Italy 0039 0363 45710
Japan 81 3 5566 0612
Lonza Distributors and Sales Offices
Poland 48 22 833 87 45
Singapore 65 64914214 For current information on Lonza distributors and sales
Spain 34 902 531 366 offices, use our website, www.lonza.com/dist, or contact
Sweden 020 140 4410 your local Lonza distributor to place your order. In the
Switzerland 0800 83 86 20 event that you do not have an authorized distributor,
The Netherlands 0800 022 4525 please contact:
United Kingdom 44 118 979 5234
Lonza Verviers, S.p.r.l.
Fax Verviers, Belgium
International Offices Phone: 00 32 87 321 611
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Austria 0800 201 536 E-mail: info.europe@lonza.com
Belgium 00 32 87 321 634
Brazil 55 11 2274 00 51 Scientific Support
Denmark 45 43 56 74 03
France 0800 91 19 80 Providing world-class technical support for our products
Germany 0800 182 52 78 is a top priority. Our Scientific Support Representatives rely
India 91 22 6697 2888 on years of laboratory experience to assist with product
Italy 39 0363 344063 selection and help you maximize product performance.
Japan 81 3 5566 0619 You may reach Scientific Support by phone or e-mail:
Poland 48 22 669 39 67 – Phone: 49(0)221-99199-400
Singapore 65 62616182
Spain 34 902 531 363 – E-mail:
Sweden 45 43 56 74 03 – scientific.support.eu@lonza.com
Switzerland 0800 83 86 19 – Full product information, instructions, and MSDS are
The Netherlands 0800 022 4942 also available at www.lonza.com/research.
United Kingdom 44 118 936 9441
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Terms and Conditions
Definitions Returns
These Terms and Conditions apply to Lonza Rockland, Inc., Lonza Walkersville, Products may not be returned for credit without first obtaining authorization
Inc. and Lonza Sales Ltd, as the case may be, herein referred to as “Seller” from a Scientific Support or Customer Service Representative. A restocking
and to all products ordered from the Lonza Cell Discovery Research Products fee may be applied for products returned without authorization or for
Catalog 2009-2010. returns in which the purchaser is at fault. The restocking fee is $50 or 25%
of the purchase price, whichever is greater.
Pricing and Terms
Visa®, American Express®, and MasterCard® credit cards are accepted. Prices Force Majeure
and conditions are subject to change without notice. The price applicable Failure of Seller to make, or purchaser to take, any one or more deliveries
to any order accepted by Seller shall be the price in effect on the date of when due, if caused by (a) fire, storm, flood, strike, lockout, accident, act of
shipment. Payment is due within 30 days following the invoice date. There war or terrorism, riot, civil commotion, embargo or similar circumstances,
is a minimum $50 order requirement for all orders. All claims by purchaser (b) any regulation, law, or restriction of any governmental department,
shall be made by written notice to Seller in accordance with these Terms commission, board, bureau, agency, court, or other instrumentality of any
and Conditions, and no offset or deduction from any invoice is permitted. supranational organization of sovereign states, country, state, province,
Acceptance by Seller of bank draft, check, or other media of payment is territory, commonwealth, municipality, or other political subdivision thereof
subject to immediate collection of the full face amount thereof. (a "Governmental Authority"), any seizure or requisition of product by any
Governmental Authority, or any compliance with a demand or request for
If at any time the financial responsibility of purchaser, or the credit risk such product for purposes of national or supranational defense, (c) inability
involved, shall become unsatisfactory to Seller, Seller may require cash or of Seller to obtain any required raw material, energy source, equipment,
satisfactory security prior to subsequent shipments or deliveries hereunder. labor or transportation, at prices and on terms deemed (by Seller) to be
The election by Seller to require such cash or security shall not affect the practicable, from Seller's usual sources of supply, or (d) any other cause
obligation of purchaser to take and pay for the contracted materials. Purchaser or contingency beyond the reasonable control of that party (whether
agrees to pay all costs and expenses, including reasonable attorneys' fees, or not of the same kind or nature as the causes or contingencies above
incurred by Seller in the collection of any sum payable by purchaser to Seller. enumerated), shall not subject the party failing to perform to any liability to
the other during the period such inability to make or take delivery shall exist.
If purchaser breaches any term of the Terms and Conditions or any other Quantities so affected may, at the option of either party, be eliminated from
contractual obligation in favor of Seller, (a) Seller may choose to defer any or the purchase without liability, but the Terms and Conditions shall remain
all further shipments or other performance of any other contractual obligation otherwise unaffected.
in favor of purchaser until purchaser cures its breach, and (b) Seller may, by
delivery of written notice to purchaser describing the breach, immediately In the event of Seller's inability, for any reason, to supply the quantities of
terminate any other contractual obligation to purchaser; provided that product contemplated by the purchase, Seller may allocate its available
purchaser shall have ten (10) days after receipt of the written notice to supply among its purchasers, including departments and divisions of Seller,
reinstate any terminated contractual obligations by curing the breach. In on such basis as Seller may deem fair and practical without liability to
the event of a termination, all outstanding payment obligations or other purchaser for any failure of performance that may result therefrom.
indebtedness of purchaser to Seller shall be due and payable no later than
fifteen (15) days after delivery of notice of termination, subject to the right
of reinstatement.
Indemnification
Seller will make available to purchaser a Material Safety Data Sheet (MSDS)
for each product delivered to purchaser where required. The MSDS sets
Notwithstanding any provision in these Terms and Conditions, Seller shall
forth information concerning such product and describes precautions, if
have no obligation to pay any rebate, issue any credit or make any other
required, to be taken in the transportation, delivery, unloading, discharge,
payment of any kind to purchaser unless purchaser is fully in compliance
storage, handling and use of such product. Purchaser will familiarize itself
with its payment and other obligations under the purchase and any other
with all information and precautions, including but not limited to such
contractual obligation in favor of Seller. In addition, in the event that purchaser
related to safety and health, contained in MSDSs or otherwise transmitted
fails to make any payment when due, Seller shall have the right to offset any
to purchaser by Seller at any time. Seller will instruct its employees, agents,
and all outstanding payment obligations or other indebtedness of purchaser
contractors, customers or any third party which may be exposed to the
to Seller against any outstanding payment obligations or other indebtedness
product about such information and precautions and make available copies
that Seller or any of its affiliates may owe purchaser.
thereof to such parties. Purchaser assumes full liability and responsibility
for compliance with the above-referenced information and precautions, and
Taxes with all laws, statutes, ordinances and regulations of any Governmental
In addition to the purchase price, purchaser shall pay Seller any and all Authority applicable to the processing, transportation, delivery, unloading,
governmental sales taxes of every kind that Seller may be required to pay discharge, storage, handling, sale and use of each product including, without
with respect to the products. Purchaser shall provide Seller, on request, with limitation, the Foreign Corrupt Practices Act and United States export control
properly completed exemption certificates for any tax from which purchaser laws; in particular, without limiting the generality of the foregoing, purchaser
claims exemption. shall not resell or ship to persons on the Denied Parties List or located within
embargoed countries (in both cases as defined under the referenced export
control laws). Purchaser further agrees to protect, defend and hold harmless
Shipping Seller from and against all claims, demands, causes of action, damages,
All orders are shipped via Seller’s recommended carriers unless an alternative
losses, liabilities, costs, expenses (including reasonable attorneys' fees),
agreement has been arranged with the purchaser. Applicable freight
penalties, and judgments (each, a "Claim") associated with the processing,
and handling charges will be added to purchaser’s invoice. Refrigerated
transportation, delivery, unloading, discharge, storage, handling, sale or use
shipments will be charged a nominal fee. Please consult a Customer Service
of any product after delivery which is (i) inconsistent with any information
Representative for confirmed delivery dates. If another carrier is specified, a
provided to purchaser or (ii) in violation of any applicable law, statute,
carrier account number must be provided by purchaser.
ordinance or regulation of any Governmental Authority. Seller assumes no
liability for failure of discharge or unloading implements or materials used
Damaged or Missing Product by purchaser whether or not supplied by Seller.
Contact a Customer Service Representative for replacement of damaged
or missing product. Damage or discrepancies must be reported within 5 Since Seller has no control over purchaser's (or others') use, disposition,
business days after receipt of product. subsequent processing, admixing or reaction of Seller’s products with other
products, chemicals or materials, purchaser assumes the entire liability
and responsibility therefor and agrees to protect, defend and hold harmless
Seller from and against all Claims associated therewith including, without
limiting the generality of the foregoing, Claims associated with infringement
of any third party's intellectual property rights, patents on processes
practiced by purchaser or patents on products made by purchaser.
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Terms and Conditions
Terms and Conditions
World – Lonza Rockland, Inc. / Lonza Walkersville, Inc. / Lonza Sales Ltd
SELLER MAKES NO WARRANTY OF ANY KIND, EITHER EXPRESS OR IMPLIED, Please note that Lonza´s Amaxa® Nucleofector® Technology is not
BY FACT OR LAW, OTHER THAN SELLER'S (I) OBLIGATION TO DELIVER PRODUCT intended to be used for diagnostic purposes, for testing or treatment in
COMPLYING WITH SELLER'S PUBLISHED SPECIFICATIONS (OR AS OTHERWISE humans.
REFERENCED IN THE TERMS AND CONDITIONS) AND (II) IMPLIED WARRANTIES
OF TITLE, FREEDOM FROM ENCUMBRANCE, AND RIGHT TO TRANSFER SAME. The Nucleofector® Technology, comprising Nucleofection® Process,
SELLER MAKES NO WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE, OR Nucleofector® Device, Nucleofector® Solutions, Nucleofector® 96-well
WARRANTY OF MERCHANTABILITY OTHER THAN AS STATED HEREIN. Shuttle® System, and 96-well Nucleocuvette® plates and modules is
covered by patent and/or patent pending rights owned by Lonza.
Seller guarantees the performance of Clonetics® and Poietics® Cells up to
two years from purchase only if appropriate Clonetics® or Poietics® Media Amaxa, HiFect, maxFP, maxGFP, Nucleocuvette, Nucleofection,
and Reagents are used exclusively, and the recommended storage and use Nucleofector, pmaxCloning, XpressNOW and 96-well Shuttle are either
protocols are followed. Cell and media performance is not guaranteed if any registered trademarks or trademarks of Lonza in Germany and/or the
modifications are made to such cell systems. U.S. and/or other countries.
The CMV promoter is covered under the U.S. patents 5,168,062 and
Limitation of Liability 5,385,839 and its use is permitted for research purposes only. Any other
SELLER SHALL NOT BE LIABLE FOR ANY DAMAGES OR INJURY TO PERSONS
use of the CMV promoter requires a license from the University of Iowa
OR PROPERTY ARISING FROM THE PURCHASE OR USE OF THE PRODUCT. IN
Research Foundation, 214 Technology Innovation Center, Iowa City, IA.
ADDITION, SELLER IS NOT LIABLE FOR THE PRODUCT AFTER THE PRODUCT
EXPIRATION DATE OR FOR A PRODUCT THAT HAS BEEN MISUSED OR HAS BECOME
Products contain a proprietary nucleic acid coding for a proprietary
UNUSABLE DUE TO IMPROPER STORAGE OR HANDLING BY PURCHASER.
fluorescent protein(s) intended to be used for research purposes only.
Any use of proprietary nucleic acid or fluorescent proteins coding by
SELLER'S TOTAL LIABILITY AND PURCHASER'S EXCLUSIVE REMEDY FOR ANY
proprietary nucleic acid other than for research use is strictly prohibited.
CAUSE OF ACTION ASSOCIATED WITH THE PURCHASE, WHETHER BASED IN
USE IN ANY OTHER APPLICATION REQUIRES LICENSE FROM EVROGEN. To
TORT, CONTRACT, STRICT LIABILITY OR ANY OTHER LEGAL THEORY IS EXPRESSLY
obtain such a license, please contact Evrogen atlicense@evrogen.com.
LIMITED TO REPLACEMENT OF NONCONFORMING PRODUCT OR PAYMENT IN AN
AMOUNT NOT TO EXCEED THE PURCHASE PRICE OF THE SPECIFIC PRODUCT
ATCC® and the ATCC Catalog Marks are trademarks of ATCC used under
FOR WHICH DAMAGES ARE CLAIMED, AT SELLER'S OPTION. IN NO EVENT SHALL
License.
SELLER BE LIABLE FOR ANY OTHER DAMAGES INCLUDING, WITHOUT LIMITATION,
INCIDENTAL, SPECIAL, PUNITIVE OR CONSEQUENTIAL DAMAGES.
Freedom EVO®, Freedom EVOware®, Tecan® and the Tecan logo are in
major countries a registered trademark of Tecan Group Ltd., Männedorf,
PURCHASER SHALL INSPECT THE PRODUCT SUPPLIED HEREUNDER
Switzerland.
IMMEDIATELY AFTER DELIVERY. PURCHASER'S FAILURE TO GIVE NOTICE TO
SELLER OF ANY CLAIM WITHIN FIVE (5) DAYS AFTER THE DATE OF DELIVERY
BD FACSCalibur is a trademark of Becton, Dickinson and Company.
SHALL CONSTITUTE UNQUALIFIED ACCEPTANCE OF THE PRODUCT AND A
WAIVER BY PURCHASER OF ALL CLAIMS WITH RESPECT THERETO.
Other product and company names mentioned herein are the trademarks
of their respective owners.
Resale
Products may be resold only by the Seller or its affiliate companies and
authorized distributors. A list of authorized distributors and affiliate
companies can be found in the back of this catalog or on our website,
Governing Law for orders made with Lonza Walkersville,
www.lonza.com. Inc. or Lonza Rockland, Inc.
The purchase and Terms and Conditions shall take effect and be construed
in accordance with the laws of the State of New York, USA.
Product Label
Please read the product label carefully for safety information and warnings
related to product hazards. Some products may present flammable, toxic, or Governing Law for orders made with Lonza Sales Ltd
other hazards. More information regarding certain products can be obtained The Purchase and Terms and Conditions shall take effect and be construed
by reading the MSDS, available on request from Customer Service, Scientific in accordance with the laws of Switzerland, without any reference to its
Support or on the web at www.lonza.com. The absence of a warning must conflicts of law.
not be construed as an indication that the product is safe. All possible
hazards may not be known at this time. Some of our products may contain
materials of animal or human origin. Products should only be handled and
used by qualified personnel familiar with the potential hazards and trained
in laboratory procedures. The purchaser assumes all risks of use and/or All rights reserved.
handling. Copyright Lonza 2008.
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Trademarks
The following are trademarks of Lonza Group or its affiliates.
Trademarks
96-well Shuttle® FastLane® maxFP® OGM™ ProEVA PAK™ SkBM®
ABM™ FBM® maxGFP® OPGM™ ProFreeze™ SkGM®
AdipoRed™ FGM® MBM® OsteoAssay™ ProHT™ StellARay™
AggreSolve™ gene transfer begins here™ MDE® OsteoImage™ ProMEDIA Select® SmBM®
AGM™ Genetic Technology Grade™ MEBM® OsteoLyse™ ProNSO™ SmGM®
Amniochrome® Global Patern Recognition™ MEGM® PC-1™ ProPer™ ToxiLight®
Amaxa® GS System™ MGM® PDELight® ProVero™1 Transport™
ApoGlow® GS Gene Expression System™ MsBM™ PGM™ PyroGene® UltraCHO™
BEBM® HBM™ MsGM™ PKLight® PYROGENT® UltraCULTURE™
BEGM® HCM™ MSCGM™ pmaxCloning™ PYROSPERSE® UltraDOMA-PF™
BioPro™ 1 HiFect® MycoAlert® PNGM™ QCL-1000® UltraDOMA™
BioWhittaker® HL-1™ MycoZap™ Poietics® ReagentPack™ UltraMEM™
BulletKit® HMM™ Nephros™ PowerCHO® REBM™ UltraMDCK™
CalciFluor™ HPGM™ NHEPS® PPiLight® REGM™ UltraPAK™
CDM™ Insect-XPRESS™ NPBM™ PrEBM™ Reliant® Versalinx®
CGM™ KBM® NPMM™ PrEGM™ SABM™ ViaLight®
Clonetics® KGM® Nucleocuvette® PrimeFect™ SAGM™ WinKQCL®
EBM® Latitude® Nucleofection® Pro293™ SCBM™ X-VIVO™
EGM® LGM-3® Nucleofector® ProCHO™ SCGM™
ExPro™ Lymphochrome™ OBM™ ProDoma™ SingleQuots®
Lonza Group or its affiliates are owner or licensee** of the following Trademarks from other companies:
patents. Products described herein may be covered by one or more ABI Prism, GeneScan, and True Allele are registered trademarks of PE Corporation,
Applied Biosystems Division
of these United States patents, by pending patent applications, or by
ALFexpress is a registered trademark of Amersham Pharmacia Biotech AB.
corresponding patents or applications in other countries.
Amplitaq, GeneAm and Fugene are registered trademarks of Roche Molecular
4,695,548** 5,219,923 6,365,341 Systems, Inc.
4,749,620* 5,641,626** 6,414,136 ATCC are trademarks of ATCC used under License.
BAX is a registered trademark of Qualicon Inc., a subsidiary of E.I. du Pont de Nemours
4,861,448** 5,836,445 6,464,850 and Co.
5,055,517 6,004,767 6,558,521 BD FACSCalibur is a registered trademark of Becton, Dickinson and Company
5,143,646 6,117,293 6,599,711 Bio-Rad, CHEF-DR, Gene Pulser, Kaleidoscope, PROTEAN, MiniPROTEAN, Ready Gel, and
Sub-Cell are registered trademarks of Bio-Rad Laboratories, Inc.
5,212,299** 6,328,870 7,166,692 Cobe is a registered trademark of Cobe Laboratories, Inc.
D510,770 D511,386 D524,449 Centipede, Millipede, and EasyCast and trademarks of Thermo Fisher Scientific, Inc.
6,521,456 7,320,859 7,332,332 C-Flex is a registered trademark of Seeman Plastics, Inc.
CrossLaps is a registered trademark of Osteometer Biotech A/S
5,168,062 5,385,839
EndoFree and siRNA are registered trademarks of Qiagen
United Kingdom patent: EndoTrap is a registered trademark of Profos, AG
2,357,336 B Eppendorf is a registered trademark of Eppendorf AG
European patents: Freedom EVO, Freedom EVOware, Tecan are registered trademarks Tecan Group, Ltd
Ficoll-Paque PLUS is a registered trademark of Amersham Bioscience/GE Helthcare
1297119 1383901 1390518 FisherBiotech is a registered trademark of Deutsche Bank, AG
1476537 1525900 1522587 Gibco, GlutaMax, Lipofectamine and Stealth are registered trademark of Invitrogen
1702677 1741778 PCT/EP01/07348 GeneSeive, StellARray and Global Pattern Recognition are trademarks of Bar Harbor
BioTechnology, Inc.
PCT/DE02/01489 PCT/DE02/01483 Hoefer is a registered trademark of Hoefer Scientific, Inc.
LI-COR is a registered trademark of LI-COR, Inc.
Matrigel is a registered trademark of Discovery Labware, Inc.
Neupogen is a registered trademark of Amgen, Inc.
Pluronic is a registered trademark of BASF Corporation
PER.C6 is a registered trademark of Crucell Holland, BV
Rainbow is a registered trademark of GE Healthcare UK
Retronectin is a registered trademark of TaKaRa Shuzo Company, LTD.
RNALater is a registered trademark of Ambion, Inc.
SeeBlue is a registered trademark of Novel Experimental Technology Corporation
SpectrMax is a registered trademark of Molecular Devices
Steady-Glo is a registered trademark of Promega
STYROFOAM is a registered trademark of Dow Chemical Corporation
SYPRO, SYBR, Alexa Fluor, Cell Tracker, Lysotracker, Mitotracker, Live/Dead, SYTO, SYTOX,
and Vybrant are registered trademarks of Molecular Probes Corporation, Inc.
Tecan, Freedom EVO and Freedom EVOware are registered trademarks of Tecan Group Ltd.
Transwell is a registered trademark of Corning, Inc.
Tween is a trademark of ICI Americas, Inc.
Versene is a registered trademark of Dow Chemical Company
Windows, Windows XP, Windows 2000 is a registered trademark of Microsoft Corporation
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Notes
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Clonetics® and Poietics®
Primary Cells and Media
Chapter 1
Clonetics® and Poietics® Primary Cells and Media
Clonetics® and Poietics® Quick Reference Guide 3 Poietics® Stem Cells & Media
WARNING: CLONETICS® AND POIETICS® PRODUCTS CONTAIN HUMAN SOURCE MATERIAL, TREAT AS POTENTIALLY INFECTIOUS.
Each donor is tested and found non-reactive by an FDA approved method for the presence of HIV-1, Hepatitis B Virus, and Hepatitis C Virus.
Where donor testing is not possible, cell products are tested for the presence of viral nucleic acid from HIV-1, Hepatitis B Virus, and Hepatitis C
Virus. Testing cannot offer complete assurance that HIV-1, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be
handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products, as recommended in the CDC-NIH Manual, “Biosafety in
Microbiological and Biomedical Laboratories”, 1999. If you require further information, please contact your site Safety Officer or Scientific Support.
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Clonetics® and Poietics® Primary Cells and Media
Cell Types - Quick Reference
Tissue/ Cell Type Cell Cat. No. Recommended Medium Media Cat. No. Page
Adventitial Fibroblasts
Aorta CC-7014 SCGM™ CC-3205 37
Astrocytes
Human Brain CC-2565 AGM™ CC-3186 50
C57 Mouse Brain - Mixed M-AsM-330 AGM™ CC-3186 76
Primary
Rat Brain - Hippocampus R-HiAs-521 AGM™ CC-3186 75
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Clonetics® and Poietics® Primary Cells and Media
Continued
Tissue/ Cell Type Cell Cat. No. Recommended Medium Media Cat. No. Page
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Clonetics® and Poietics® Primary Cells and Media
Continued
Tissue/ Cell Type Cell Cat. No. Recommended Medium Media Cat. No. Page
Primary
Rat Brain - Striatum R-Cp-502 PNGM™ CC-4461 74
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Clonetics® and Poietics® Primary Cells and Media
Continued
Hematopoietic Cells
Cell Type Source Peripheral
Blood Bone Marrow Cord Blood Fetal Liver Page
Fresh Bone Marrow 1M-125 83
Mesenchymal Stem Cells PT-2501 90
CD4 T Cells
+ 2C-200 2W-200 95
CD14+ Monocytes 2W-400 93
CD19+ B Cells 2C-300 95
CD34+ Cells 2M-101 2C-101 2L-101 86 & 87
Cells and Media
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Clonetics® Primary Cells and Media
In vivo relevance. In vitro results.
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Clonetics® Conditionally Immortalized Cell Lines
During recent years, increased pressure has been Characterization for Cell-based Drug Discovery
placed on drug development teams to deliver products
Lonza has qualified the conditionally immortalized cell
that meet the prescribed claims, lack side effects, and
lines for use in high throughput, cell-based functional
perform despite the patients' genetic background. To add
assays. This offers an excellent method to identify novel
to the challenge, drug discovery resourcing has not been
compounds that target insulin resistance, for example.
growing at the same rate as the external costs required to
Compounds can be rapidly identified in such screens to
validate new drugs for the market. Now, more than ever,
produce a lead series that can be integrated into drug
pharmaceutical researchers need tools that facilitate HTS
discovery pipelines.
cell screening in complex biological systems earlier in the
Immortalized Cells
process in order to eliminate poor drug candidates early As part of the characterization of our most promising
Conditionally
and allow focus on more promising candidates. conditionally immortalized human muscle cell lines, we
screened a diverse chemical library, assaying for effects
To discover a new drug, many compounds may be
on glycogen synthesis. This screen identified a novel
screened or evaluated for efficacy and toxicity. Several
series of GSK3 inhibitors.
accepted models exist (using either animals or cells), but
all have inherent limits that delay expedient evaluation
Future Products
using high throughput screening techniques. They also
require species extrapolation to evaluate the data. We are currently developing a set of conditionally
immortalized cell lines from the major insulin target tissues
Live animal models yield a rich supply of data, but
i.e. muscle, visceral and subcutaneous adipocytes.
are not cost effective for high throughput screening.
These human cell lines, optimized for diabetes research
Non-immortalized, primary cells may best represent
and high throughput cell-based assays, originate from
normal physiology, but are typically not available in the
only ethically obtained tissues.
large, uniform quantities needed for HTS applications.
Though conventionally immortalized cell lines can divide For more information concerning licensing opportunities, please call
301-898-7025, ext. 7350.
indefinitely into large homogeneous cell populations
needed for HTS applications, they do not behave like
normal cells. Conditional immortalization allows for, at Conditional Immortalization
a permissive temperature, production of high volumes
of uniform cell populations. Changing the culture 37°C – Normal Phenotype
temperature forces the cells to stop dividing, allowing Cells isolated from
for differentiation and maturation so that the cells can human tissues
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Clonetics® Cell Models
Cell Models
for many years to evaluate and classify a wide range of
chemicals and cosmetic products. The Draize test involves — ET50 values established using 0.3% Triton X-100
the placement of substances directly into the conjunctival — Measurable IL-1a increases in the culture medium
sac of rabbit eyes in vivo with subsequent evaluation for relative to time of irritant exposure
irritation and damage. Ethical concerns calling for the use
— Cultures achieve stratification of 4 – 9 cell layers with
of alternative methods to animal testing and uncertainty
an average of 6 – 7 layers
regarding inter-species comparisons between rabbits
and humans have led to an increased focus on the use of — Supplied ready-to-use in Corning Transwell® Inserts
human tissue models for screening purposes. In order to
create a physiologically relevant in vitro human corneal
epithelial culture model, it is desirable that cells used ■ Product Applications
be of human origin (species-specific) and isolated from – Ocular irritancy evaluation for:
corneal epithelial tissue (tissue-specific). – Pharmaceutical products
– Pharmaceutical raw materials
Due to the difficulty in obtaining consistent high-quality – Personal care products
primary human corneal epithelial cells in sufficient – Cosmetics
numbers, the use of cell lines presents an – Standard household use chemicals
attractive alternative. However, many continuous – Basic research – corneal epithelium
cell lines lose phenotypic and/or morphological
– Ocular diseases
characteristics of the native tissue, and therefore
– Barrier function studies
do not provide a relevant system of study. We have
overcome these difficulties by the introduction ■ Routine QC Testing
of human papilloma virus 16 E6 and E7 (HPV-16 E6/E7) – Trans Epithelial Electrical Resistance – TEER is a
genes into the cells. Through the use of HPV-16 E6/E7 measure of the barrier function of epithelial cellular
in primary human corneal epithelial cells, we have junctions. The tighter the epithelial barrier, the higher
developed functional human corneal epithelial cells the TEER value, expressed as “ohms × cm2” or “Ω × cm2”.
with extended lifespan.
– ET50 (0.3% Triton X-100 as test substance)
— The Clonetics® Human Corneal Epithelial Culture (For Information Only, available upon request)
Model provides a consistent and robust platform for
References
recapitulation of human corneal epithelial tissue in an
in vitro environment 1. Draize, JH., Appraisel of the Safety of Chemicals in Foods, Drugs and
Cosmetics 1959. The Association of Food and Drug officials of the
— The tissue model achieves a histological profile United States. Austin, Texas.
2. Wilhelmus, KR., The Draize Eye Test. Survey of Ophthalmology 2001
similar to that observed with in vivo corneal epithelium May-June 45 (6):495-514.
3. Halbert, CL., Demers, GW., and Galloway, DA. The E7 Gene of Human
— The formation of epithelial junctions, essential in Papillomavirus Type 16 is sufficient for immortalization of human
establishing proper barrier function, is demonstrated epithelial cells. 1991. J. Virol 65 (1): 473-478.
4. Griffith, M., Watsky, MA., and Liu, C., Trinkaus-Randall V. Epithelial Cell
through High TEER values which reflect relevant Culture. Cornea in Methods of Tissue Engineering. ed. Atala, A., Lanza,
barrier properties and RP. 2002 Academic Press. 131-140.
5. Toropainen, E., Ranta, VP., Talvitie, A., Suhonen, P., and Urtti, A. Culture
— The human corneal epithelial culture model is predictive model of human corneal epithelium for prediction of ocular drug
absorption. Invest Ophthalmol Vis Sci. 2001 Nov 42 (12): 2942-8.
of in vivo ocular irritancy via multiple endpoints and 6. Eye Irritation: Reference Chemicals Data Bank. ECETOC Technical Report
exhibits good correlation with Draize scores No. 48, 2nd Edition. June 1998.
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Clonetics® Cell Models
Continued
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Clonetics® Cell Models
Continued
Cell Models
Cat. No. Size Price
REGM™ BulletKit®
Includes Basal Medium and SingleQuots® Kit
1 Lot 1 Lot 2 Lot 3
CC-3190 Kit
Ratios of apical-to-basal expression of γ-GTP were averaged for three lots
of renal cortical epithelial cells in two separate experiments (triplicate wells for REBM™ Basal Medium
each lot).
CC-3191 500 ml
Apical uptake
Basal uptake
Uptake of rhodamine 123 was assessed from both apical and basal culture
medium. Fluorescence of extracted rhodamine 123 was normalized to
apical concentration in each experiment. Results are from three lots of
renal cortical epithelial cells (triplicate wells for each lot) in two separate
experiments.
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Clonetics® Cell Models
Continued
— Tested for the absence of bacterial, mycoplasmal, – DiI-acetylated LDL for endothelial cells
yeast and fungal contamination, and for viability and – Calcein AM assay for P-glycoprotein (P-gp) function,
growth after thawing using verapamil hydrochloride as a P-gp inhibitor
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Clonetics® Cell Models
Continued
Cell Models
50
EMVB BulletKit®
Includes Basal Medium and SingleQuots®
AC-3103 Kit
0 0 µM 25µM
Verapamil HCI Concentration EBM®-2 Endothelial Cell Basal Medium-2
Calcein AM Efflux for P-gp Function for 3 lots of cells using
Verapamil HCl as a P-gp inhibitor. CC-3156 500 ml
10
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Hepatocytes and Media
Clonetics® NHEPS® Normal Human and Animal Primary Hepatocytes are General Ordering and Shipping Information
available cryopreserved, as fresh suspension cultures, or freshly plated in Cryopreserved cells and media products are
flasks or multiwell plates. Hepatocytes are also available as cell pellets in normally shipped Monday – Thursday for next
day delivery. Saturday and Monday deliveries
RNALater®, a reagent that inactivates RNases and stabilizes RNA within unfrozen are available upon special request.
tissues and cells. Plated hepatocytes are supplied on collagen or Matrigel®
For notification of the availability of plated
coated substrates. Other matrices and formats are offered as products through human hepatocytes, contact Customer
our Custom Laboratory. Lonza offers Clonetics® Optimized Media for the Service or your local Sales Representative.
culture of hepatocytes to provide consistent and maximum performance. All For delivery of plated hepatocytes outside the
hepatocytes are guaranteed to perform as indicated when used with Clonetics® continental United States, please contact your
local agent as to feasibility/availability.
Media and Reagents. All media lots are function-tested on the appropriate
Hepatocytes
and Media
Human hepatocytes are obtained and handled in a legal and ethically responsible
manner. Lonza’s animal facilities are AAALAC Accredited.
Human Hepatocytes
albumin expression
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Human Hepatocytes and Media
Continued
Cell Type Description Cryopreserved Plated Seeding Density Viable Time in Culture
h NHEPS® Human Primary Primary ≈150,000 cells/cm 2 6 to 12 hours in suspension,
hepatocytes 4 to 5 days plated
Hepatocytes
and Media
h NHEPS® Human
HCM™ BulletKit®
CC-2591 3 – 6 × 106 viable cells/ampoule Includes Basal Medium and SingleQuots®
CC-3198 Kit
h NHEPS® Suspension Cells (fresh)
(5 × 10 cells minimum order)
6
HBM™ Basal Medium
CC-2690 5 × 106 cells
CC-3199 500 ml
h NHEPS® Flasks – Collagen
HCM™ SingleQuots® Supplements and Growth Factors
CC-2694 T-25 flask
CC-2695 T-75 flask CC-4182 —
h NHEPS® Multiwell Plates – Collagen For more information about these media systems, see page 115. For
more information regarding media selection, please contact Scientific
Support at 1-800-521-0390. Matrigel® or collagen is required for culturing
CC-2691A 6 wells
hepatocytes.
CC-2692A 12 wells
Please inquire for additional formats.
CC-2693A 24 wells
CC-2698A 96 wells
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Ready Heps™ Fresh Human Hepatocytes
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Rat Hepatocytes – Sprague-Dawley and Media
Hepatocytes
and Media
(Phase 1, EROD and ECOD)
Cell Type Description Cryopreserved Plated Seeding Density Viable Time in Culture
rt NHEPS® Hepatocytes, rat, Primary From primary 150,000 cells/cm2 7 days
Sprague-Dawley
rt NHEPS® Multiwell Plates – Matrigel®, pooled Please inquire for additional formats.
AC-2623B 24 wells
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Airway Epithelial Cells and Media
Airway cells are used in the functions of respiration and mucus production.
Clonetics® Human Airway Cells are available as:
— SAEC - Human Small Airway Epithelial Cells – Isolated from the distal portion
of the lung in the 1 mm bronchiole area
— NHBE - Normal Human Bronchial/Tracheal Epithelial Cells – Isolated from the
epithelial cells that line the airway above the bifurcation of the lungs
■ Research Applications NHBE - Excellent packed cuboidal morphology
– Cystic Fibrosis – Drug uptake
– Cancer – Air /Liquid interface
Cells and Media
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Airway Epithelial Cells and Media
Continued
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Airway Epithelial Cells and Media
Continued
For more information about these media systems, see page 112
Related Products
BSMC - Normal Human Bronchial Smooth Muscle Cells 66
HMVEC-L - Human Lung Microvascular Endothelial Cells 26
HMVEC-LLy - Human Lung Lymphatic Microvascular 27
Endothelial Cells
NHLF - Normal Human Lung Fibroblasts 39
Cell Culture Reagents 78
PrimeFect™ Transfection Reagents 203
NHDF - Nucleofector® Kit 151
NHDF - 96-well Nucleofector® Kit 151
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Bladder Cells and Media
The bladder serves as a reservoir for water soluble byproducts generated during
cell metabolism. Clonetics® Bladder Cells are available as: BdSMC - Human
Bladder Smooth Muscle Cells and HMVEC-Bd - Human Bladder Microvascular
Endothelial Cells – isolated from the surrounding bladder tissue.
■ Research Applications
– Overactive bladder
– Cancer HMVEC-Bd
– Urologic disease
Bladder
– BdSMC stain positive for smooth muscle alpha actin and negative for von
Willebrand Factor
– HMVEC-Bd stain positive for von Willebrand Factor and LDL and negative for
smooth muscle alpha actin
– Guaranteed through 10 population doublings when using Clonetics® Media HMVEC-Bd
and Reagents
■ Media and Supplements
– Use SmGM®-2 Smooth Muscle Growth Medium-2 for BdSMC Morphological Performance
– Use EGM®-2MV Microvascular Endothelial Growth Medium-2 for HMVEC-Bd Criteria
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured ■ No multinucleation
in our appropriate media system ■ Cells not enlarged
Ordering information on next page ■ Minimal vacuolation
References
— For a comprehensive list of
references and additional
technical information, please
visit www.lonza.com/research
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Bladder Cells and Media
Continued
SmGM®-2 SingleQuots® Supplements and Growth Factors EGM®-2MV SingleQuots® Supplements and Growth Factors
CC-4149 — CC-4147 —
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Endothelial Cells and Media
Endothelial cells are the membrane or monolayer lining of cells taken from blood
vessel, heart, lymphatic, surface spinal cord and brain, or anterior eye chamber
of normal human tissue.
■ Research Applications
– Atherosclerosis – Inflammation
HAEC
– Angiogenesis – Wound healing
– Arteriosclerosis – Drug uptake or drug discovery
– Oncology – Cell-to-cell junctions
– Positive for von Willebrand Factor Expression/Factor VIII; positive stain ■ Cells refractile
reflects a healthy endothelial cell; von Willebrand Factor synthesis is critical ■ High mitotic index
to blood clotting
■ Small, similar sized cells
– PECAM-positive for lung microvascular cells; stains for adherent junctions
■ No pericytes or smooth muscle
that endothelial cells use to control passage of molecules across the tissue
cells or enlarged cells visible
– HUVEC characterized by morphological observation only
– Up to 15 population doublings guaranteed when using Clonetics® Media and
Reagents; individual cell types may vary
■ Media and Supplements
References
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured
in our appropriate media — For a comprehensive list of
references and additional
– Use EGM® or EGM®-2 Medium Endothelial Media with large vessel cell types
technical information, please
– EGM®-2 Medium is a more defined medium with only 2% FBS and several visit www.lonza.com/research
growth factors replacing BBE - Bovine Brain Extract
– EGM®-MV and EGM®-2MV Media are optimized for use with microvascular
endothelial cells
– The media systems are offered as BulletKit® Products (basal medium and
separately packaged growth factors)
– Provides flexibility to manipulate media components specific to your application
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Endothelial Cells and Media
Continued
HMVEC-dAd Adult dermal microvascular 3rd passage 4th or 5th passage 5,000 cells/cm2 5 to 9 days
Endothelial
HMVEC-dBIAd Adult dermal blood 3rd passage 4th or 5th passage 5,000 cells/cm 2 4 to 7 days
microvascular
HMVEC-dBINeo Neonatal dermal blood 3rd passage 4th or 5th passage 5,000 cells/cm2 4 to 7 days
microvascular
HMVEC-dLyAd Adult dermal lymphatic 3rd passage 4th passage 5,000 cells/cm2 4 to 7 days
microvascular
HMVEC-dNeo Neonatal dermal microvascular 3rd passage 4th or 5th passage 5,000 cells/cm2 5 to 9 days
HMVEC-dLyNeo Neonatal dermal lymphatic 3rd passage 4th passage 5,000 cells/cm2 4 to 7 days
microvascular
HPAEC Pulmonary artery 3rd passage 4th passage 2,500 – 5,000 cells/cm2 5 to 9 days
HUAEC Umbilical artery 3rd passage 4th passage 2,500 – 5,000 cells/cm2 5 to 9 days
HUVEC Umbilical vein 1st passage 2nd passage 2,500 – 5,000 cells/cm2 5 to 9 days
UtMVEC-Myo Uterine microvascular 3rd passage 4th or 5th passage 5,000 cells/cm 2 5 to 9 days
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Endothelial Cells and Media
Continued
Endothelial
CC-3162 Kit CC-3202 Kit
Proliferating Cells – Flasks – in EGM®-2 Medium Proliferating Cells – Flasks – in EGM®-2MV Medium
CC-2635 T-25 flask CC-2685 T-25 flask
CC-0222 T-75 flask CC-0261 T-75 flask
CC2535T150 T-150 flask CC2585T150 T-150 flask
CC2535T225 T-225 flask CC2585T225 T-225 flask
Proliferating Cells – Multiwell Plates – in EGM®-2 Medium Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium
CC-2535W6 6 wells CC-2585W6 6 wells
CC-2535W12 12 wells CC-2585W12 12 wells
CC-2535W24 24 wells CC-2585W24 24 wells
CC-2535W48 48 wells CC-2585W48 48 wells
CC-0132 96 wells CC-0188 96 wells
HAEC – Human Aortic Endothelial Cells HCAEC – Human Coronary Artery Endothelial Cells
Cat. No. Size Price
Cat. No. Size Price
Proliferating Cells – Flasks – in EGM® Medium
Proliferating Cells – Flasks – in EGM® Medium
C2585AT25 T-25 flask
C2535AT25 T-25 flask
C2585AT75 T-75 flask
C2535AT75 T-75 flask
C2585AT150 T-150 flask
C2535AT150 T-150 flask
C2585AT225 T-225 flask
C2535AT225 T-225 flask
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Endothelial Cells and Media
Continued
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
Endothelial
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Endothelial Cells and Media
Continued
Endothelial
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
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Endothelial Cells and Media
Continued
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium Proliferating Cells – Multiwell Plates
CC-2543W6 6 wells CC-2810W6 6 wells
CC-2543W12 12 wells CC-2810W12 12 wells
CC-2543W24 24 wells CC-2810W24 24 wells
CC-2543W48 48 wells CC-2810W48 48 wells
CC-2543W96 96 wells CC-2810W96 96 wells
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Endothelial Cells & Media
Continued
Endothelial
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
CC-3202 Kit CC-3202 Kit
Proliferating Cells – Multiwell Plates Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium
CC-2811W6 6 wells CC-2505W6 6 wells
CC-2811W12 12 wells CC-2505W12 12 wells
CC-2811W24 24 wells CC-2505W24 24 wells
CC-2811W48 48 wells CC-2505W48 48 wells
CC-2811W96 96 wells CC-0112 96 wells
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Endothelial Cells and Media
Continued
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
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Endothelial Cells and Media
Continued
Endothelial
Includes Basal Medium and SingleQuots® Kit
CC-3202 Kit
CC-3202 Kit
Proliferating Cells – Flasks
Proliferating Cells – Flasks – in EGM®-2MV Medium
CC-7030T25 T-25 flask
CC-2616 T-25 flask CC-7030T75 T-75 flask
CC-0288 T-75 flask CC-7030T150 T-150 flask
CC2516T150 T-150 flask CC-7030T225 T-225 flask
CC2516T225 T-225 flask
Proliferating Cells – Multiwell Plates
Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium
CC-7030W6 6 wells
CC-2516W6 6 wells CC-7030W12 12 wells
CC-2516W12 12 wells CC-7030W24 24 wells
CC-2516W24 24 wells CC-7030W48 48 wells
CC-2516W48 48 wells CC-7030W96 96 wells
CC-2516W96 96 wells
Ordering information continues on next page
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Endothelial Cells and Media
Continued
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Endothelial Cells and Media
Continued
Endothelial
CC-3124 Kit
CC-3125 Kit
Proliferating Cells – Multiwell Plates Proliferating Cells – Multiwell Plates – in EGM® Medium
CC-2517W6 6 wells
CC-2520W6 6 wells
CC-2517W12 12 wells
CC-2520W12 12 wells
CC-2517W24 24 wells
CC-2520W24 24 wells
CC-2517W48 48 wells
CC-2520W48 48 wells
CC-0124 96 wells
CC-0085 96 wells
HUAEC – Human Umbilical Artery Endothelial Cells Cat. No. Size Price
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Endothelial Cells and Media
Endothelial
Phenol Red Free
CC-4176 —
CC-3129 500 ml
EGM®-2MV BulletKit®
Includes Basal Medium and SingleQuots® Kit
EGM® SingleQuots® Supplements and Growth Factors
CC-3202 Kit
CC-4133 —
EGM®-2MV SingleQuots® Supplements and Growth Factors EGM®-MV BulletKit®
Includes Basal Medium and SingleQuots® Kit
CC-4147 —
CC-3125 Kit
EGM® Complete Medium
(Supplemented)
EGM®-MV SingleQuots® Supplements and Growth Factors
CC-3024 500 ml
CC-4143 —
For more information about these media systems, see page 113 –114
Endothelial cells must be cultured in their isolation medium for best results.
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Fibroblast Cells and Media
Fibroblasts are found in the stroma of tissue, where they play several important
roles, such as manufacturing growth factors and protein fibers.
■ Research Applications
– Fibrosis – Fibrosarcoma – Hypertension
– Toxicology – Cell signaling – Heart disease
– HPdLF stain negative for pan cytokeratin ■ Cells elongated and fibrous
appearance
– NHLF and NHDF guaranteed through 15 population doublings when using
Clonetics® Media and Reagents ■ Refractile like smooth muscle
cells
– AoAF and HPdLF guaranteed through 10 population doublings when using
Clonetics® Media and Reagents ■ High mitotic index
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Fibroblast Cells and Media
Continued
Fibroblast
Cells Ordering Information Cells Ordering Information
AoAF – Human Aortic Adventitial Fibroblasts HPdLF – Human Periodontal Ligament Fibroblasts
Recommended Medium: SCGM™ BulletKit®, see page 39 Recommended Medium: SCGM™ BulletKit®, see page 39
Cat. No. Size Price Cat. No. Size Price
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Fibroblast Cells and Media
Continued
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
Fibroblast
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Fibroblast Cells and Media
Continued
Fibroblast
CC-3132 Kit
CC-4126 —
Proliferating Cells – Flasks
FGM® BulletKit®
CC-2612 T-25 flask Includes Basal Medium and SingleQuots® Kit
CC-0282 T-75 flask CC-3130 Kit
CC2512T150 T-150 flask
FGM® SingleQuots® Supplements and Growth Factors
CC2512T225 T-225 flask
CC-4134 —
Proliferating Cells – Multiwell Plates
SCGM™ BulletKit®
CC-2512W6 6 wells Includes Basal Medium and SingleQuots® Kit
CC-2512W12 12 wells CC-3205 Kit
CC-2512W24 24 wells
CC-2512W48 48 wells SCBM™ Basal Medium
CC-0164 96 wells CC-3204 500 ml
For more information about these media systems, see pages 115, 120
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Epidermal Keratinocyte Cells and Media
■ Research Applications
– Epithelial cell model – Cancer
– Wound healing – Drug efficacy
NHEK - 100% confluent
– Burn therapy – Immunology
– Dermatology disorders
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Epidermal Keratinocyte Cells and Media
Continued
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Epidermal Keratinocyte Cells and Media
Continued
Note: Cells isolated in KGM®-2 should only be cultured in KGM®-2. Cells isolated in KGM® can be cultured in KGM® or KGM®-2.
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Epidermal Keratinocyte Cells and Media
Continued
Keratinocyte
Includes Basal Medium and SingleQuots® Kit
Includes Basal Medium and SingleQuots® Kit
CC-3107 Kit
CC-3111 Kit
Note: Cells isolated in KGM®-2 should only be cultured in KGM®-2. Cells isolated in KGM® can be cultured in KGM® or KGM®-2.
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Epidermal Keratinocyte Cells and Media
Continued
KGM®-CD SingleQuots® Supplements and Growth Factors KGM® SingleQuots® Supplements and Growth Factors
Keratinocyte
CC-4456 —
CC-4131 —
KGM®-2 BulletKit® KGM® BulletKit® w/o Ca++
Includes Basal Medium and SingleQuots® Kit
Includes Basal Medium and SingleQuots® Kit
CC-3107 Kit
CC-3112 Kit
KBM®-2 Basal Medium KBM® Basal Medium w/o Ca++
CC-3103 500 ml
CC-3104 500 ml
KGM®-2 SingleQuots® Supplements and Growth Factors KGM® Complete System (supplemented)
CC-4152 — CC-3001 500 ml
KGM®-2 BulletKit® w/o Ca ++
ReagentPack™ Subculture Reagents
Includes Basal Medium and SingleQuots® Kit
Trypsin/EDTA, Trypsin Neutralizing Solution, and HEPES Buffered Saline
CC-3108 Kitl Solution
KBM®-2 w/o Ca++ Basal Medium CC-5034 100 ml each
CC-3158 500 ml
Related Products
Cell Culture Reagents 78
PrimeFect™ Transfection Reagents 203
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Epidermal Melanocyte Cells and Media
Clonetics® Normal Human Epidermal Melanocytes are neural crest derived cells
that reside in the basal stratum of the epidermis. These cells are responsible
for producing and storing melanin that, through interaction with keratinocytes,
provide pigment and UV protection to the skin. Clonetics® Melanocyte Cells are
sourced from:
— Human neonatal foreskins available as single donors
— Human adult tissue Mel-5 staining
■ Research Applications:
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Epidermal Melanocyte Cells and Media
Continued
Competitive Analysis of Clonetics® Melanocyte Cells and Medium Media Ordering Information
250,000
Proliferation (RLU)
50,000
CC-4435 —
0 Clonetics A
®
Clonetics B
®
Clonetics C
®
Competitor
Melanocyte Lots Phosphate Buffered Saline (PBS)
Proliferation comparison of 3 unique lots of Clonetics® NHEM - Normal 17-516F 500 ml
Human Epidermal Melanocytes cultured in MGM®-4 Medium were compared
with 1 lot of the competitor’s NHEM culture in the competitor’s growth
medium. Cell proliferation was measured using Vialight® Plus (reported Trypsin/EDTA Solution
as relative luminescent units RLU), which detects ATP levels indicative of
viable cells. Each bar represents a unique lot of Clonetics® NHEM. Results of CC-5012 100 ml
2 unique MGM®-4 Medium lots were averaged with an n = 6 per replicate.
Versene® (EDTA) 0.02%
Cells Ordering Information 17-711E 100 ml
NHEM-Neo – Neonatal Normal Human Epidermal
Trypsin Neutralizing Solution (TNS)
Melanocytes
Recommended Medium: MGM®-4 BulletKit® CC-5002 100 ml
Proliferating Cells – Flasks For more information about this medium, see page 117
CC-2504T25 T-25 flask
CC-2504T75 T-75 flask
CC-2504T150 T-150 flask
CC-2504T225 T-225 flask
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Mammary Epithelial Cells and Media
Clonetics® HMEC - Human Mammary Epithelial Cells are isolated from human
adult breast tissue.
■ Applications
– Breast cancer
– Cellular function and differentiation
– Physiology HMEC 95% confluent
– Toxicology
Mammary Epithelial
Cells and Media
■ Cell Testing and Specifications
– HMEC test positive for cytokeratins 14 and 18, and negative for cytokeratin 19
– Guaranteed through 15 population doublings when using Clonetics® Media
and Reagents
References
— For a comprehensive list of
references and additional
technical information, please
visit www.lonza.com/research
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Mammary Epithelial Cells and Media
Continued
Cat. No. Size Price Includes Basal Medium and SingleQuots® Kit
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Neural Cells and Media
Clonetics® Neural Cells are used to study the function of the central nervous
system. Cells are available as:
— NHA - Normal Human Astrocytes
■ Research Applications
– Neurogenesis research – Pharmacology
– Cell physiology – Parkinson’s Disease NHA stained positive for GFAP by indirect
immunofluorescence staining
– Injury – Astrocyte-mediated neurotoxicity
Neural
■ Cell Testing and Specifications
– NHA test positive for GFAP
– NHA guaranteed through 10 population doublings when using Clonetics® Morphological Performance
Media and Reagents Criteria
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Neural Cells and Media
Continued
CC-3186 Kit
Cryopreserved Cells
CC-2565 ≥1,000,000 cells/ampoule ABM™ Basal Medium
CC-3187 500 ml
AGM™ BulletKit®
Includes Basal Medium and SingleQuots® Kit
AGM™ SingleQuots® Supplements and Growth Factors
CC-3186 Kit
CC-4123 —
Proliferating Cells – Flasks
CC-2665 T-25 flask
ReagentPack™ Subculture Reagents
Trypsin/EDTA, Trypsin Neutralizing Solution, and HEPES Buffered Saline
CC-0297 T-75 flask Solution
CC2565T150 T-150 flask CC-5034 100 ml each
CC2565T225 T-225 flask
For more information about medium, see page 155
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Prostate Cells and Media
Prostate
■ Cell Testing and Specifications
– PrEC test positive for cytokeratin (clone 8.13)
– PrSC test positive for vimentin and negative for pan cytokeratin
– Both types of prostate cells guaranteed through 15 population doublings
PrSC stained for vimentin
when using Clonetics® Media and Reagents
– PrSMC stain positive for alpha actin
– PrSMC are guaranteed to 10 population doublings when using Clonetics®
Media and Reagents
Morphological Performance
■ Media and Supplements Criteria
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured ■ Cells rounded and not flat
in our appropriate media ■ Cells shiny and refractile
– Use PrEGM™ Prostate Epithelial Cell Growth Medium for PrEC
■ High mitotic index
– Use SCGM™ Stromal Cell Growth Medium for PrSC
■ No enlarged cells visible
– Use SmGM®-2 Smooth Muscle Growth Medium-2 for PrSMC
– The media systems are offered as BulletKit® Products (basal medium and
separately packaged growth factors)
– Provides flexibility to manipulate media components specific to your application References
Ordering information on next page — For a comprehensive list of
references and additional
technical information, please
visit www.lonza.com/research
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Prostate Cells and Media
Continued
PrEC – Human Prostate Epithelial Cells PrSC – Human Prostate Stromal Cells
Recommended Medium: PrEGM™ BulletKit® Recommended Medium: SCGM™ BulletKit®
Cat. No. Size Price Cat. No. Size Price
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Prostate Cells and Media
Continued
Prostate
CC-3182 Kit
SCGM™ SingleQuots® Supplements and Growth Factors
Proliferating Cells – Flasks
CC-4181 —
CC-2587T25 T-25 flask
For more information about this medium, see page 159
CC-2587T75 T-75 flask
CC2587T150 T-150 flask
CC2587T225 T-225 flask
PrSMC Prostate Smooth Muscle Cell Media
Proliferating Cells – Multiwell Plates Cat. No. Size Price
CC-2587W6 6 wells
SmGM®-2 BulletKit®
CC-2587W12 12 wells Includes Basal Medium and SingleQuots® Kit
CC-2587W24 24 wells CC-3182 Kit
CC-2587W48 48 wells
CC-2587W96 96 wells SmBM® Basal Medium
CC-3181 500 ml
Related Products
Cell Culture Reagents 78
PrimeFect™ Transfection Reagents 203
PrEC - Nucleofector® Kit 162
PrEC - 96-well Nucleofector® Kit 162
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Renal Cells and Media
Renal cells are found in the kidneys. They eliminate waste products and modulate
electrolytes, pH and blood plasma volume. Clonetics® Human Renal Cells are
available as:
— HRE - Human Renal Epithelial Cells
— HRCE - Human Renal Cortical Epithelial Cells
— RPTEC - Human Renal Proximal Tubule Epithelial Cells
— NHMC - Normal Human Mesangial Cells RPTEC stained positive for γ-GTP
■ Research Applications
Cells and Media
– Physiology – Glomerulonephritis
Renal
– Guaranteed through 15 population doublings when using Clonetics® Media – Grow as cell swirl
and Reagents – Slightly refractile
■ Media and Supplements ■ HRCE
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured – Cobblestone appearance
in our appropriate media which can surround RPTEC
and mesangial cells if these
– Use REGM™ Renal Epithelial Growth Medium for HRE, RPTEC and HRCE populations are present
– Use MsGM™ Mesangial Cell Growth Medium for NHMC ■ NHMC
– The media systems are offered as BulletKit® Products (basal medium and – Mesangial cells are large, flat
separately packaged growth factors) cells with more cytoplasm and
– Provides flexibility to manipulate media components specific to your irregular borders
application
References
— For a comprehensive list of
references and additional
technical information, please
visit www.lonza.com/research
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Renal Cells and Media
Continued
Renal
Recommended Medium: REGM™ BulletKit®, see page 57 Recommended Medium: REGM™ BulletKit®, see page 57
Cat. No. Size Price Cat. No. Size Price
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Renal Cells and Media
Continued
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
CC-3190 Kit
CC-3146 Kit
Renal
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Renal Cells and Media
Continued
Renal
REGM™ SingleQuots® Supplements and Growth Factors MsGM™ SingleQuots® Supplements and Growth Factors
CC-4127 — CC-4146 —
For more information about these media systems, see page 118
Related Products
Cell Culture Reagents 78
PrimeFect™ Transfection Reagents 203
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Skeletal Cells and Media
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured – Spindle morphology when
in our appropriate media confluent
– Use OGM™ Osteoblast Growth Medium and OGM™ Differentiation SingleQuots® – Healthy osteoblasts mineralize
Kit to expand and differentiate NHOst under differentiation conditions
– Use CGM™ Chondrocyte Growth Medium and CDM™ Chondrocyte ■ Chondrocytes
Differentiation Medium to expand and differentiate NHAC-kn – Spindle becoming mostly
– We supply sodium alginate, calcium chloride, sodium chloride, and sodium polygonal when confluent
citrate, required reagents for culture and differentiation of our chondrocytes
– The media systems are offered as BulletKit® Products (basal medium and
separately packaged growth factors) References
– Provides flexibility to manipulate media components specific to your — For a comprehensive list of
application references and additional
technical information, please
visit www.lonza.com/research
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Skeletal Cells and Media
Continued
Skeletal
Recommended Medium: OGM™ BulletKit®, see page 60 Recommended Medium: CGM™ BulletKit® for Growth, CDM™ BulletKit® for
Differentiation, see page 60
Cat. No. Size Price
Cat. No. Size Price
Cryopreserved Cells
Cryopreserved Cells
CC-2538 ≥500,000 cells/ampoule
CC-2550 ≥750,000 cells/ampoule
OGM™ BulletKit®
Includes Basal Medium and SingleQuots® Kit CGM™ BulletKit®
Includes Basal Medium and SingleQuots® Kit
CC-3207 Kit
CC-3216 Kit
Proliferating Cells – Flasks
CDM™ BulletKit®
CC-2538T25 T-25 flask Includes Basal Medium and SingleQuots® Kit
CC-2538T75 T-75 flask CC-3225 Kit
CC2538T150 T-150 flask
CC2538T225 T-225 flask Proliferating Cells – Flasks
CC-2550T25 T-25 flask
Proliferating Cells – Multiwell Plates
CC-2550T75 T-75 flask
CC-2538W6 6 wells CC2550T150 T-150 flask
CC-2538W12 12 wells CC2550T225 T-225 flask
CC-2538W24 24 wells
CC-2538W48 48 wells Proliferating Cells – Multiwell Plates
CC-2538W96 96 wells
CC-2550W6 6 wells
CC-2550W12 12 wells
Cell Pellet in RNALater®
CC-2550W24 24 wells
PX-2538RL ≈10 million cells/pellet CC-2550W48 48 wells
CC-2550W96 96 wells
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Skeletal Cells and Media
OGM™ SingleQuots® Supplements and Growth Factors CDM™ SingleQuots® Supplements and Growth Factors
CC-4193 — CC-4408 —
For more information about these media systems, see page 119
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Skeletal Muscle Cells and Media
Skeletal muscle cells form the muscles that coordinate with the skeletal system
of the body, where their contractions result in bodily movement. Clonetics®
Skeletal Muscle Cells are offered as:
— SkMC - Human Skeletal Muscle Cells are isolated as satellite cells, from the
upper arm or upper leg
— HSMM - Human Skeletal Muscle Myoblasts are isolated from post-gestational
tissue, usually from quadriceps or psoas tissue SkMC stained for Desmin
■ Research Applications
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Skeletal Muscle Cells and Media
Continued
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Skeletal Muscle Cells and Media
Continued
DPBS
17-512F 500 ml
For more information about these media systems, see page 157
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Smooth Muscle Cells and Media
Smooth muscle cells are found in many parts of the body, where they work
by controlling the movement of blood, food or air. Clonetics® Smooth Muscle Cells
are obtained from:
— Aorta — Pulmonary artery
— Major bronchia — Umbilical artery
— Coronary artery — Uterine wall
UtSMC stained for smooth muscle a-actin
■ Research Applications
Cells and Media
Smooth Muscle
– Stain positive for a-actin and negative for von Willebrand Factor after
differentiation Morphological Performance
– Guaranteed through 15 population doublings when using Clonetics® Media Criteria
and Reagents
■ Refractile but less so than
■ Media and Supplements epithelial cells
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured ■ Elongated and sometimes
in our appropriate media triangular in shape
– Use SmGM®-2 Smooth Muscle Growth Medium–2 with 5% FBS ■ High mitotic index
– The media systems are offered as BulletKit® Products (basal medium and ■ No cytoskeleton visible
separately packaged growth factors)
– Provides flexibility to manipulate media components specific to your
application References
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Smooth Muscle Cells and Media
Continued
SmGM®-2 BulletKit®
Includes Basal Medium and SingleQuots® Kit
CC-3182 Kit
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
CC-3182 Kit CC-3182 Kit
Related Products
Cell Culture Reagents 78
PrimeFect™ Transfection Reagents 203
Smooth Muscle Cell Nucleofector® Kits 172 - 173
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Smooth Muscle Cells and Media
Continued
PrEGM™ BulletKit®
Includes Basal Medium and SingleQuots® Kit
CC-3166 Kit
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Smooth Muscle Cells and Media
Continued
Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
CC-3182 Kit CC-3182 Kit
SmGM®-2 BulletKit®
Includes Basal Medium and SingleQuots®
CC-3182 Kit
For more information about this medium, see page 120 BdSMC - Human Bladder Smooth Muscle Cells 65
Cell Culture Reagents 53
PrSMC - Human Prostate Smooth Muscle Cells 85
PrimeFect™ Transfection Reagents 203
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Animal Cells and Media
Clonetics® Animal Primary Cells are provided with the same quality standards General Ordering and Shipping Information
as the Clonetics® Human Cell products. All cells are performance tested and test Cryopreserved cells and media products are
negative for mycoplasma, bacteria, yeast and fungi. Clonetics® Cells are guaranteed normally shipped Monday – Thursday for next
day delivery. Saturday and Monday deliveries
to perform as indicated when used with Clonetics® Cells, Media and Reagents. are available upon special request.
Immuno and special staining protocols, as well as characteristic morphology,
Proliferating cell orders must be placed no
are used to characterize the cells and assure they are the designated type. A later than Tuesday at 5 p.m. for delivery the
Certificate of Analysis is available for each cell type. Most cells are available following Monday. Orders placed after Tuesday
will be held until the next production cycle.
cryopreserved or proliferating, in either flasks for plates, or as a cell pellet in As you place your order for proliferating cells,
RNALater®, a reagent that inactivates RNases and stabilizes RNA within unfrozen please order the appropriate media.
Endothelial
days. Please plan accordingly.
— Bovine Endothelial Cells
— Rat Cardiac Myocytes
— Rat and Mouse Neurons
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Endothelial Cells and Media
Continued
bAEC – Bovine Aortic Endothelial Cells, Single Donor bAEC – Bovine Aortic Endothelial Cells, Pooled
Endothelial
Recommended Medium: EGM® MV BulletKit®, see page 31 Recommended Medium: EGM® MV BulletKit®, see page 31
Cat. No. Size Price Cat. No. Size Price
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Endothelial Cells and Media
Continued
Endothelial
CC-3125 Kit $113 CC-3125 Kit
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Rat Cardiac Myocytes and Media
Each lot of these cells test positive for functional syncytium formation, and stain
Rat Cardiac Myocytes
Lonza now offers fully optimized cardiac myocyte medium and growth factors
to complement Clonetics® Rat Cardiac Myocytes as a complete system to study
cardiac biology or toxicology. Clonetics® RCGM Rat Cardiac Myocyte Growth
Medium is specifically formulated for the growth and survival of rat neonatal
cardiac myocytes in culture.
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Primary Neural Cells and Media
Convenient – You can avoid today’s inefficiencies to conduct neuronal research Cortical and striatal rat neurons
Cells were thawed, co-cultured 21 days,
■ Applications
– Transfection
– Evaluation of electrophysiological properties, neurotransmitters, receptor
function
– Research typical inhibitory or excitatory ion-channels
– Receptor signaling research
– Intracellular transport studies
– Neurotoxicity research
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Primary Neural Cells and Media
Clonetics® Cryopreserved Neurons from rat brain are cell suspensions of high
quality primary embryonic brain neuronal cells (including glia) prepared by
standardized methods and are ready for immediate culture.
approximately 4 million viable cells. Each vial (0.25 ml) of hippocampus neurons
contain approximately 1 million viable cells.
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Primary Neural Cells and Media
Continued
Clonetics® Ready-to-use Astrocytes are obtained from rat brain, passaged once,
and prepared as cell suspensions for shipment on dry ice. Each vial (1 ml)
contains approximately 1 million cells. These astrocytes can be simply thawed and
cultured. Following confluence, the astrocytes can be harvested for re-plating or
stored frozen for later use.
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Primary Neural Cells and Media
Continued
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Primary Neuron Growth Media
Optimized medium for culturing primary neurons
Primary Neuron
necessary volumes of L-glutamine, Penicillin/Streptomycin,
Growth Media
and NSF-1 — a supplement supporting neuronal growth CC-3256 500 ml
AGM™ BulletKit®
Includes Basal Medium and SingleQuots® Kit
CC-3186 Kit
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Cell Culture Reagents
The following products are cell culture tested using human primary cells.
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Custom Cell Services
— Cell characterization including functional testing ■ We deliver the cells with medium and culture reagents
and staining of your choice
— Cell performance testing including growth and ■ We take care of details, you get results
differentiation
— ISO 9001:2000 Certified quality management
company
— cGMP compliant manufacturing available upon
request
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Notes
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Poietics® Stem Cells
We do the isolation, you do the research
Poietics® Stem Cells and Media include a collection General Ordering and Shipping Information
of human stem and progenitor cells derived from Differentiating cells are generally available in both fresh and cryopreserved
hematopoietic, adipose, and neural systems. Fresh or formats. Orders for fresh cells are usually scheduled 1 – 2 weeks
ahead of your anticipated use date. Fresh cells are processed on the
cryopreserved cells are available from bone marrow, same day as drawn and shipped for overnight delivery. Fresh and
cord blood, and fetal liver. Media systems are available for cryopreserved cells are shipped Monday through Thursday. Fresh cells are
shipped in HBSS with 5 mM EDTA and 0.5% BSA.
the cell types. All cells are obtained through Institutional
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Donor Programs
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Bone Marrow and Mononuclear Cells
Unprocessed bone marrow orders are shipped Monday Cells Ordering Information
through Thursday and are shipped the same day the bone
Cat. No. Size Price
marrow is drawn for delivery on the following morning.
Please contact your Scientific Support Representative for Unprocessed Human Bone Marrow
(Fresh)
further details on ordering fresh marrow.
1M-105 10 ml
Our high quality human bone marrow is guaranteed to 1M-125 25 ml
contain a minimum of 15 million leukocytes per ml. Order 4 × 1M-125 100 ml
(same donor)
Mononuclear Cells
Bone Marrow and
Poietics® Normal Human Peripheral Blood Leukopak is Cells Ordering Information
a bag of fresh human blood cells collected from normal
Cat. No. Size Price
peripheral blood by automated apheresis in ACD-A
anticoagulant and density gradient centrifugation. Each Normal Human Peripheral Blood Leukopak
leukopak contains a mixture of monocytes, lymphocytes, 1A-125 100 ml
platelets, plasma, and red cells. Leukopaks offer large
quantities of cells from a single donor which can be useful
in research for isolation of novel cell types, applications of
novel isolation methods, and the various other applications
associated with blood and immune cell research.
■ Features
– Monocyte enriched
– Standard leukopaks have a volume up to 100 ml and a
total white cell count ≥60 million per ml
– NOTE: Smaller volumes available upon request
– All donors are screened for general health, and test
negative for HIV-1, Hepatitis B & C
– A product information sheet is provided with each
leukopak
– PMN-enriched leukopaks available upon request
– Leukopaks with special donor characteristics are
available, such as CMV negative and EBV
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Bone Marrow and Mononuclear Cells
cells are available from a large and varied donor pool. Human Bone Marrow Mononuclear Cell Panel
(cryopreserved, 5 donors)
2M-125B 25 million cells/donor
Human Cord Blood Mononuclear Cells
Hematopoietic progenitors exist in higher quantities in
umbilical cord blood than adult peripheral blood. Due to
fewer histocompatibility problems and greater access to Cells Ordering Information
ethnic diversity, cord blood cells are of great interest in
Cat. No. Size Price
transplantation and ex vivo expansion research. Cells are
available cryopreserved. Human Cord Blood Mononuclear Cells
(cryopreserved)
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Bone Marrow and Mononuclear Cells
The Quadratox 4 Species Panel is a panel of bone marrow- Cells Ordering Information
derived mononuclear cells from four different mammalian
Cat. No. Size Price
species including inbred C57BL/6 mice, purebred beagles,
baboons, and humans. Animals and tissues are obtained Quadratox 4 Species Panel
(cryopreserved)
from AAALAC-accredited facilities.
2S-101 ≥5 million cells/species
Quadratox Panels can be used to assess the relative
toxicities of chemotherapeutic drugs in pre-clinical Human Bone Marrow
(cryopreserved)
research. Inter-species differences in the relative toxicities of
2S-101D ≥5 million cells
numerous chemotherapeutic drugs are well documented.
In vitro toxicity data have been shown to contribute to Murine Bone Marrow
both the structuring and subsequent interpretation of (cryopreserved)
clinical trials. Quadratox Panels may be used for selection 2S-101C ≥5 million cells
of the most appropriate animal models for both efficacy
and toxicology studies and prediction of in vivo human Canine Bone Marrow
(cryopreserved)
Mononuclear Cells
Bone Marrow and
myelotoxicity.
2S-101B ≥5 million cells
The Quadratox 4 Species Panel is available as cryo-
Baboon Bone Marrow
preserved vials of ≥5 million cells/species. Bone marrow (cryopreserved)
mononuclear cells from human, murine, canine, and
2S-101A ≥5 million cells
baboon are also available individually.
References
— For a comprehensive list of references and additional
technical information, please visit www.lonza.com/
research
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Hematopoietic Progenitor Cells and Media
The cell surface glycoprotein CD34 is a known marker Cells Ordering Information
for hematopoietic progenitor cells. As these cells mature
Human Bone Marrow CD34+ Progenitor Cells
and differentiate, CD34 expression is lost. CD34+ cells CD34+ Progenitor Cell purity is ≥95%.
are capable of initiating long-term hematopoiesis both in
vitro and in vivo. CD34+ Progenitor Cells are available from Cat. No. Size Price
bone marrow, cord blood, and fetal liver. They are isolated Human Bone Marrow CD34+ Progenitor Cells
from mononuclear cells using positive immunomagnetic (fresh)
selection. Analysis by flow cytometry is included. 1M-101 ≥ 100,000 cells
1M-101A ≥ 300,000 cells
Fresh cells need to be scheduled 1 – 4 weeks in advance 1M-101B ≥ 500,000 cells
depending on the source and volume requirements. 1M-101C ≥ 1,000,000 cells
RFU
4.0E+05
2M-101 ≥ 100,000 cells
Hematopoietic
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Hematopoietic Progenitor Cells and Media
Hematopoietic
progenitor cells (CD34+ cells), with cytokines. HPGM™ Hematopoietic Progenitor Growth Medium
CD34+ cells can be stimulated to undergo a limited PT-3926 500 ml
amount of proliferation and still maintain a relatively
undifferentiated phenotype when grown in this medium.
Additionally, HPGM™ can be used to support the growth
and/or differentiation of other hematopoietic progenitor
cells such as erythroid progenitors, CD133+ cells, and
mononuclear cells.
CD133 is selectively expressed on CD34+ bright cells and Cells Ordering Information
is a subset of hematopoietic progenitor cells. CD133+ cells
Cat. No. Size Price
are isolated from human bone marrow, umbilical cord
blood, and fetal liver by positive immunoselection. Human Bone Marrow CD133+ Progenitor Cells
(cryopreserved)
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Hematopoietic Progenitor Cells and Media
lineages.
Hematopoietic
80
70
60
50
40
30
20
10
References
— For a comprehensive list of references and additional
technical information, please visit www.lonza.com/
research
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Hematopoietic Progenitor Cells and Media
When bone marrow cells are put into culture under specific Cells Ordering Information
conditions, a stromal layer of adherent cells develops over
Cat. No. Size Price
a few weeks. The stromal layer is composed of many cell
types, including fibroblasts, mesenchymal stem cells, Human Bone Marrow Non-Irradiated Stromal Cells
(cryopreserved)
adipocytes, endothelial cells and macrophages. These
stromal cells function as a feeder layer for hematopoietic 2M-302 ≥5 million cells
progenitors, allowing proliferation and differentiation of
progenitors to continue for weeks in these cultures with
no addition of exogenous cytokines. This long-term culture
system allows researchers to study an in vitro model of
the bone marrow microenvironment, including cell-cell
interactions, adhesion molecules and cytokine secretion.
These and many other factors allow for the tight regulation
of blood cell production, progenitor cell commitment and
Hematopoietic
References
— For a comprehensive list of references and additional
technical information, please visit www.lonza.com/
research
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Human Mesenchymal Stem Cells and Media
Poietics® hMSC Mesenchymal Stem Cells are harvested Cells Ordering Information
and cultured from normal human bone marrow. Cell
Cat. No. Size Price
purity is far higher than cells from stromal cell cultures.
Cells are tested for purity by flow cytometry and for their hMSC Human Mesenchymal Stem Cells
(cryopreserved)
ability to differentiate into osteogenic, chondrogenic,
and adipogenic lineages. Cells are positive for CD105, PT-2501 ≥750,000 cells
CD166, CD29, and CD44. Cells test negative for CD14,
CD34, and CD45. Media systems are available to support Media Ordering Information
growth of hMSCs and their differentiation into adipogenic, Mesenchymal Media
chondrogenic, and osteogenic lineages.
Cat. No. Size Price
References MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit®
Includes basal medium and SingleQuots® Kit
— For a comprehensive list of references and additional
technical information, please visit www.lonza.com/ PT-3001 Kit
research MSCGM™ Mesenchymal Stem Cell Basal Medium
Mesenchymal stem cells are licensed by Lonza from Osiris Therapeutics, PT-3238 440 ml
Inc. and are subject to the following limited use license:
The included biological material, including progeny and derivatives, MSCGM™ Mesenchymal Stem Cell Growth Medium
(collectively referred to as “Material”) is licensed to you under specific
Stem Cells and Media
terms. You are responsible for ensuring that the terms of the license SingleQuots® Kit
agreement are met.
Mesenchymal
PT-4105 —
1. Grants of License: Lonza Walkersville, Inc. grants you a nontransferable,
nonexclusive license to use the Material for research.
2. Not for Human Use: The Material may not be used: a) in humans; b) in hMSC Mesenchymal Stem Cell Chondrogenic Differentiation
conjunction with human clinical trials; c) in association with human BulletKit®
diagnostics. Includes Basal Medium and SingleQuots® Kit
3. Material Not Transferable: You may not transfer the Material to any other
person or organization. PT-3003 Kit
4. Patent Notice: Material under license from Osiris Therapeutics, Inc. NOTE: TGF-β3 component sold separately
Material is covered by US patent 5,486,359 and others.
1,000
rhTGF Beta 3 for hMSC Chondrogenic Differentiation Media
1,000
Forward Scatter
Supplement
800 800
PT-4124 2 µg
600 600
PT-3004 Kit
*Refer to page 91 to see our recomended alternative media for adipocyte
differentiation media
Subculturing Reagents
DPBS
hMSC in culture
17-512F 500 ml
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Human Preadipocyte Cells and Media
adipocytes when fully differentiated. Adipocytes perform PGM™-2 Growth Medium SingleQuots® Kit
essential functions of energy metabolism and are PT-9502 Kit
characterized by the accumulation of intracellular
triglycerides. This cell system has been designed for AdipoRed™ Assay Reagent
use in high throughput applications to conduct research
PT-7009 5 × 4 ml
on lipid accumulation and metabolism, obesity, insulin
sensitivity, diabetes, and diet drugs. Subculturing Reagents
Poietics® Cells, Media, and Reagents are quality tested DPBS
together and guaranteed to give optimum performance 17-512F 500 ml
as a complete cell system. NOTE: Please inquire about availability of subcutaneous and visceral
preadipocyte sets from same donor.
2
Differentiated subcutaneous preadipocyte cells
1.5
0.5
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Human Osteoclast Precursor Cells and Media
Poietics® Osteoclast Precursor Cells and Media contain: Cells Ordering Information
— Cryopreserved Human Osteoclast Precursors Cat. No. Size Price
— OCP Osteoclast Precursor Medium BulletKit®, which Human Osteoclast Precursors
includes the basal medium and supplements needed (cryopreserved)
to induce the osteoclast precursors to differentiate into 2T-110 ≥1 million cells/amp
mature osteoclasts; these differentiated osteoclasts
stain positive for TRAP and express the calcitonin Media Ordering Information
receptor
OCP Osteoclast Precursor Media
Osteoclasts are large, multinucleated cells that play an
active role in bone resorption. This cell system has been Cat. No. Size Price
designed for use in high throughput applications to OCP Osteoclast Medium BulletKit®
conduct research on osteoporosis, bone resorption, and Includes basal medium and SingleQuots® Kit
other bone-related diseases. PT-8001 Kit
Poietics® Cells, Media, and Reagents are quality tested OCP Osteoclast Precursor Basal Medium
together and guaranteed to give optimum performance PT-8201 100 ml
as a complete cell system.
OCP Osteoclast Precursor SingleQuots® Kit
Osteoclast Precursor
PT-9501 Kit
Cells and Media
150,000
100,000
50,000
Pits formed from bone resorption activity of differentiated osteoclasts 0 Control Day 5 - 6 Days 0 - 6
Calcitonin (1nM) Exposure
Inhibition of bone matrix resorption by calcitonin. Poietics® Primary Human
Osteoclast Precursors were cultured in differentiation medium containing no
calcitonin, calcitonin added only at day 5 and calcitoinin added on days 0
and 5 and assayed after a total of 6 days. Calcitonin, added at day 0, resulted
in the osteoclasts becoming refractory to calcitonin added on day 5.
Related Products
OsteoAssay™ Human Bone Plate 237
OsteoImage™ Mineralization Assay 240
OsteoLyse™ Assay Kit (Human Collagen) 238
Calcifluor™ Quantitative Bone Resorption Assay 239
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Human Immune System Cells and Media
Normal human monocytes are found in the circulating Cells Ordering Information
peripheral blood; they play an important role in host
Cat. No. Size Price
defense as circulating monocytes and differentiation into
tissue macrophages and can differentiate into dendritic Human Peripheral Blood CD14+ Monocytes
cells with potent antigen-presenting capability, in vivo (cryopreserved)
Immune System
Cells and Media
CD14+ Monocytes
■ Media Recommendations
– The cryopreserved cells can be stored in liquid nitrogen
until they are needed. Once thawed, the cells can be
cultured in a variety of different conditions. To maintain
the monocyte phenotype, serum-containing medium is
recommended (10% FBS is generally recommended).
M-CSF (at 10 ng/ml) can also be added. To produce
osteoclasts, both soluble RANK ligand (30 ng/ml) and
M-CSF (30 ng/ml) need to be added to serum-containing
medium. To produce dendritic cells, a serum-free medium
such as LGM-3® Lymphocyte Growth Medium-3 should
be used, with the addition of GM-CSF (50 ng/ml) and
IL-4 (50 ng/ml).
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Human Immune System Cells and Media
Poietics® Normal Human Dendritic Cells and Media Cells Ordering Information
consist of:
Cat. No. Size Price
— Primary derived cultures of NHDC - Normal Human
Dendritic Cells and/or HPBMC - Human Peripheral Blood NHDC – Normal Human Dendritic Cells
Recommended Medium: LGM-3®
Mononuclear Cells (cryopreserved)
— LGM-3® Lymphocyte Growth Medium-3, a serum-free CC-2701 2.5 × 106 cells
medium for lymphocyte cell growth
This system may be used for experimental applications HPBMC – Human Peripheral Blood Mononuclear Cells
(cryopreserved)
such as drug-induced immunosuppression of cytokine
secretion, antigen presentation and uptake studies, and CC-2702 ≥50 million cells
phenotypic studies of dendritic cells. CC-2702 ≥ 100 million cells
CC-2702 ≥ 200 million cells
All cells are isolated from peripheral blood by apheresis and
density gradient centrifugation. Dendritic cells, peripheral Medium Ordering Information
Immune System
Dendritic cells
■ Media recommendations
– Poietics® LGM-3® Lymphocyte Growth Medium-3
contains albumin, insulin, transferrin, and gentamicin;
to complete NHDC differentiation, cells must be cultured
in IL-4 and GM-CSF
■ Performance and Quality Tests
– All cells test negative for HIV-1, Hepatitis B and C,
mycoplasma, bacteria, yeast, and fungi
– After 4 days in culture, the NHDC stain positive for
CD11c and CD86 surface antigens, by flow cytometric
analysis
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Human Immune System Cells and Media
Immune System
Cells and Media
Cord Blood CD19+ B Cells
Poietics® Cord Blood CD19+ B cells are isolated from cord
blood mononuclear cells using negative immunomagnetic
selection. Purity is ≥85%.
References
— For a comprehensive list of references and additional
technical information, please visit www.lonza.com/
research
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Human Immune System Cells and Media
(cryopreserved)
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Human Neural Progenitor Cells and Media
Poietics® Neural Progenitor Cells and Media contains Cells Ordering Information
NHNP-Normal Human Neural Progenitor Cells and
Cat. No. Size Price
optimized medium for their sustenance. This cell system
can be useful for research applications in drug development, NHNP - Normal Human Neural Progenitor Cells
Recommended Medium: NPMM™ BulletKit®
neurotoxicity, neurogenesis, electrophysiology, CNS
PT-2599 ≥1.2 × 106 cells/ampoule
function, and neurotransmitter disorders.
NHNP - Cell Pellet in RNALater
Poietics® NHNP Cells are cryopreserved in primary passage
as “spheroids”. We guarantee that Poietics® NHNP will PX-2599RL ≈10 million cells/pellet
express markers described when plated onto laminin
coated plates.
Neural Progenitor
Cells and Media
(astrocytes) NPDM™ Neural Progenitor Differentiation Medium BulletKit®
Includes basal medium and SingleQuots® Kit
We now offer two media kits specially formulated to CC-3229 Kit
support the growth, expansion, and differentiation of the
NHNP cells. NPBM™ Neural Progenitor Basal Medium
CC-3210 200 ml
NPMM Neural Progenitor Maintenance Medium BulletKit®
contains the necessary supplements and media for optimal NPMM™ Neural Progenitor Maintenance Medium
NHNP cell growth and differentiation. This kit includes: SingleQuots® Kit
– CC-3210 – Neural Progenitor Basal Medium CC-4241 —
– CC-4241 – Neural Progenitor Maintenance Medium Neural Progenitor Supplement SingleQuots® Kit
SingleQuots® Kit (contains hEGF and hFGF)
CC-4242 —
– CC-4242 – Neural Progenitor Supplement SingleQuots®
Kit (contains NSF-1 and GA) For related neural cell and media systems, see page 49, 73 – 76
References
1. Sold under license from StemCells, Inc. US patents 5,968,829 and
— For a comprehensive list of references and additional 5,851, 832.
technical information, please visit www.lonza.com/
research
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Custom Cell Isolation Services
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Clonetics® FAQs
Clonetics® FAQs
Antibiotics are not provided for the KGM®-CD Kit plates (with the exception of hepatocytes,
and will need to be added if you so desire. bovine brain microvascular endothelial cells, and
the rat/mouse neuronal and dorsal root ganglion
Q. Can I use a medium other than the one you offer
neurons, rat cardiac myocytes which do require a
to culture the Clonetics® Cells?
coating). However, we have found that glass
A. We have an optimized medium for each Clonetics® slides, chamber slides, and other formats may
Cell type. The cells are grown and tested in their require a coating prior to seeding the cells.
specific medium system. We cannot guarantee Please contact Scientific Support for further
cell performance if our recommended medium is information.
not used. Q. Can Clonetics® Cells be transfected?
Q. What is the difference between the EGM® A. Yes, see pages 191, 203-205 for transfection
BulletKit® (CC-3124) and the EGM®-2 BulletKit® reagents that are optimized to work with our
(CC-3162)? primary cells.
A. The primary difference between the two media Q. Your instructions do not mention centrifuging
types is in the growth factors. The EGM® the amp of cells upon thawing. Won’t the DMSO
BulletKit® contains: BBE, hEGF, hydrocortisone, kill the cells?
FBS, and GA-1000. In the EGM®-2 BulletKit®, A. Clonetics® Normal Human Cells are very
we have removed the BBE and replaced it with fragile out of cryopreservation and should
known growth factors: VEGF, hFGF-B, ascorbic not be centrifuged (exception – bovine brain
acid, insulin-like growth factor, and heparin. microvascular endothelial cells and hepatocytes).
The cells proliferate much better in the EGM®-2 Residual DMSO will be removed by a media
Medium. It has been reported that the EGM® change after 24 hours.
BulletKit® works better for VEGF, FGF, and
angiogenesis research. Q. I thawed and plated my cells yesterday and
most of the cells are floating, what happened?
A. Clonetics® Cells will not be confluent on day
one. After 24 hours, you will see few attached
cells per field under the microscope. Aspirate
off the media and the floating cells and replace
with fresh, pre-warmed media. Those attached
cells will begin to proliferate and a confluent
monolayer should be established in 5 –10 days.
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Clonetics® FAQs
Continued
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Clonetics® Technical Information: Cells and Media
Overview of cell culture process for Clonetics® Human and Animal Cells*
▼
BulletKit® Supplemented Media
Prepare Medium
▼ ▼
▼ ▼
Storage Requirements: Calculate number of flasks/
Incubate 3 – 4 hours
chambers/dishes required
Cells
Upon arrival, immediately remove cryo-
preserved cells from dry ice and place Add medium to culture vessel(s)
immediately into liquid nitrogen. If no Replace medium and incubate
and incubate for at least 30
Technical Information
dry ice is left in the package, thaw cells, minutes
immediately place them into culture
vessels and call your Technical Specialist.
Clonetics®
Medium Thaw cells in water bath
*Exceptions: NHEM, Rat and Mouse neural cells, Rat Cardiac Myocytes,
h NHEPS®, sm NHEPS®, rt NHEPS®, and bMVEC-B
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Safety Precautions with Clonetics® Cells
— As a precaution against contamination, follow all procedures for handling products of human origin outlined in
“Guidelines to Avoid Personnel Contamination By Infective Agents in Research Laboratories That Use Human Tissues,”
from the J. of Tissue Culture Methods.
— Always wear gloves and safety glasses when working with all materials. Exercise caution when working with cryopreserved
cells; rapid temperature changes may cause splattering of liquid nitrogen.
— Wash hands thoroughly after performing all procedures.
— Do not smoke, eat or drink in areas where reagents or cells are handled.
— Never pipette by mouth.
— Products of human and animal origin are potential biohazards. Although provided human cells negative for HIV-1,
Hepatitis B and C, proper precautions must be taken to avoid inadvertent exposure.
WARNING: Clonetics® and Poietics® Products contain human sourced material. Treat as potentially infectious.
Media Preparation
Technical Information
Clonetics®
Perform the following steps in a sterile field For BulletKit®, perform the following steps
For a bottle of fully supplemented medium, do the 1. Decontaminate the external surfaces of the
following: SingleQuots® Cryovials and the basal media bottle
1. Add BBE or BPE, if required, to a 500 ml bottle of with ethanol or isopropanol.
basal medium.
2. Aseptically open each cryovial and add the entire
2. Detach the BBE or BPE supplement from the medium amount to the basal medium with a pipette.
bottle.
3. Rinse each cryovial with the medium. It may not
3. Decontaminate the vial and medium bottle with be possible to recover the entire volume listed for
ethanol or isopropanol. each cryovial; small losses, even up to 10%,
4. Add the entire contents of the vial (approximately should not affect the cell growth characteristics of
2 ml) to the medium with a pipette; rinse the vial the supplemented medium.
with medium and pipette the contents back into the 4. Transfer the label provided with each kit to the basal
500 ml bottle. medium bottle being supplemented. Use it to record
5. Replace the cap and swirl the medium gently a few the date and amount of each supplement added; we
times to mix. recommend that you place the completed label over
the basal medium label to avoid confusion or possible
6. Record the date the BBE or BPE was added on the double supplementation.
medium label.
5. Record the new expiration date on the label based on
the shelf life. A fully reconstituted BulletKit® should
be used within 30 days; this supplemented medium
will now be referred to as a growth medium.
NOTE: If there is concern that sterility was compromised during the
supplementation process, the entire newly prepared growth medium may
be re-filtered to assure sterility. If you re-filter, use a sterile 0.2-micron filter.
Routine re-filtration is not recommended as some protein loss may occur
with each filtration.
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Media Preparation
Continued
Technical Information
– Hemacytometer or cell counter
Clonetics®
Growth Area of Common Plasticware
These instructions do not apply to bMVEC-B, hepatocytes, The advantage of setting up this number of T-25 flasks
NHEM, rat & mouse cells, and Poietics® Cells. from the initial cryovial, as opposed to larger flasks, is
1. Calculate the number of vessels to be set up. that it reduces the risk of losing large numbers of cells.
Refer to your Certificate of Analysis for the exact That is, if you experience difficulty trypsinizing the first
number of cells in your cryovial. Refer to table on T-25 flask, there are other remaining T-25 flasks to use.
page 103 “Growth Area of Common Plasticware”, 2. Label each flask with the passage number, cell
for help in adjusting this calculation. type, lot number, and date.
Use the following calculations to determine the number 3. In a sterile field, carefully open the supplemented
of vessels to be set-up for the recommended seeding bottle of growth medium and aseptically transfer
densities of 2,500 cells/cm2, 3,500 cells/cm2, or 5,000
the medium to new culture vessels by adding 1
cells/cm2
ml growth medium for every 5 cm2 surface area
No. of cells available × Percent viability Max. no. of cm2 that can of the flask.
=
Recommended seeding density be plated
Example: 5 ml growth medium for a 25 cm2 flask.
Max. no. of cm2 that can be plated Max. no. of flasks that 4. Tighten vented caps on vessels. If vented caps
=
Effective growth area of flask can be set up
are not being used, twist caps until tight, then
Example: A cryovial of HMVEC-L with 520,000 cells and 80% viability
loosen about 1/2 turn. Allow the culture vessels
to warm and equilibrate in a 37°C, 5% CO2,
520,000 × 0.80
= 83 cm2, to be set up humidified incubator for at least 30 minutes.
5,000
Technical Information
Aseptically add the recommended amount of medium to the color of the thawed cryovial should be pink.
the flask and equilibrate for 30 minutes in a 5% CO2, 37°C If the color is not pink, seed the cells, note the
incubator. color and mention this fact to Scientific Support
1. Have a micropipette ready prior to thawing. if seeding is not successful.
2. Remove the cryovial of cells from storage. Wipe NOTES:
cryovial with ethanol or isopropanol before – If more than one cryovial is to be thawed, thaw one cryovial
at a time and keep other cryovials in liquid nitrogen until
opening. In a sterile field, briefly twist the cap a ready for use
quarter turn to relieve the internal pressure, then – Cryopreserved cells are very delicate; thaw and return them
retighten; do not open the cryovial completely. to culture as quickly as possible with minimal handling
3. Holding the cryovial, dip the bottom 3/4 of the – Wear eye protection when handling frozen cells; rapid
temperature changes may cause splattering of liquid
cryovial in a 37°C water bath and swirl gently for nitrogen
1 – 2 minutes until contents are thawed. Watch – Centrifugation should not be performed to remove cells
the cryovial closely; when the last sliver of ice from the cryoprotectant cocktail; this action is more
damaging than the effects of DMSO residue in the culture
melts remove it; DO NOT submerge it completely.
– It is not recommended to thaw frozen cells directly onto
Thawing the cells for longer than 2 minutes may glass slides, chamber slides, gridded plates or multiwell
result in less than optimal results. plate configurations (6, 12, 24, 96…); optimal performance
is achieved when initial seeding out of cryopreservation is
4. Remove the cryovial immediately, wipe it performed into T-25 flasks; for further instructions follow
dry, and transfer to a sterile field where the the directions in the set-up section in the cell culture
instructions provided
equilibrated flasks should be waiting, ready to
seed. Rinse the cryovial with 70% alcohol, then
wipe to remove excess.
5. Note the color of the thawed cryovial. Ideally,
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Seeding – Adherent Cell Types
Remove the cap, being careful not to touch the interior After Seeding
threads with your fingers. Cells are not tolerant to rapid temperature fluctuations
1. Using a 1,000 µl micropipette set to 800 µl, put the or nutrient-deficient medium. Feeding them with fresh
tip into the cryovial and resuspend the cells with growth medium that has been warmed will avert potential
a gentle, slow and steady up and down pipetting problems. (Remember to warm only the amount needed.)
motion no more than five times. DO NOT resuspend Check and feed the cells on the schedule below, even on
quickly and keep the tip near the bottom to avoid weekends and holidays.
making bubbles. 1. Change the growth medium the day after seeding
2. Dispense an equal amount of cells into the flasks (to remove residual DMSO and unattached cells),
set up earlier (as determined by the recommended then every other day thereafter while examining
seeding density and number of cells/vial). If four them daily.
T-25 flasks were prepared, set micropipette to 250 NOTE: A change of medium requires removal of the medium by aspirating
with a sterile pipette on the opposite side of the flask from where the cells
µl and dispense. If eight T-25 flasks were prepared, are attached. Then warm, fresh medium is added down the same side.
set micropipette to 125 µl and dispense.
3. Replace the cap or cover and gently rock the 2. Successfully recovered cultures will exhibit the
vessels to evenly distribute the cells. Loosen caps if following:
a. Cells with clear non-granular cytoplasm.
necessary to permit gas exchange. b. Numerous mitotic figures after day 2.
4. Return the culture vessels to a 37°C incubator 3. Feed the cells a larger volume of medium as
with 5% CO2. Lay them flat on the shelf, providing
Technical Information
they become more confluent. Use this table as a
the largest surface for cells to attach. The cells will guideline:
anchor to the bottom surface of the flask. If cells are: Then feed them:
Clonetics®
Under 25% confluent… 1 ml per 5 cm2
NOTE: Do not dispense the entire contents of the cryovial into one T-25 From 25 – 45% confluent… 1.5 ml per 5 cm2
flask! Exceeding 45% confluence… 2 ml per 5 cm2
1. Examine the culture microscopically for any signs to 37°C in a sterile container. Warming the entire
of distress during shipment (i.e., detachment, bottle can shorten the life of the medium. Never
rounding-up, or atypical morphology). Check the warm medium under hot running water or any
relative cell density and estimate % confluency. The other uncontrolled temperature source. NEVER
culture should be 30 – 100% confluent upon receipt. MICROWAVE.
Some cellular detachment is normal. Please call 5. In a sterile field, carefully open the cell culture flask
Scientific Support immediately if cells look severely or multiwell dish, remove the medium and replace it
distressed. with the warmed, fresh medium. Aseptically remove
2. Decontaminate the external surface of the cell culture any medium inside the neck or cap area because it
flask or multiwell dish by wiping with 70% ethanol or can facilitate microbial contamination.
isopropanol. 6. If you are using a flask with a non-vented cap, loosen
3. Incubate the sealed flask or multiwell dish at 37°C, 5% the cap and return the flask to the 37°C humidified
CO2, for 3 – 4 hours to equilibrate temperature. incubator with 5% CO2 for at least 24 hours.
4. Warm an appropriate amount of growth medium
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Subculturing – Adherent Cell Types
stored at -20°C until shipped from Lonza’s Distribution 1. Aspirate the medium from one culture vessel.
Centers. 2. Rinse the cells with 5 ml of room temperature
2. Subculture reagents may thaw during transport. HEPES Buffered Saline Solution (HEPES-BSS). DO
They may be refrozen once. NOT forget this step. The medium contains complex
proteins that neutralize the trypsin.
3. Subculture reagents can be stored at -20°C until
expiration date, after thawing once and refreezing. 3. Aspirate the HEPES-BSS from the flask.
4. To keep Trypsin/EDTA fresh and active after thawing, 4. Cover the cells with 2 ml of Trypsin/EDTA solution.
you may aliquot it into sterile centrifuge tubes and 5. Tighten the cap and begin monitoring the flask
refreeze at -20°C. Trypsin/EDTA may be stored frozen under the microscope.
until the expiration date.
6. Continue to examine the cell layer microscopically.
5. We recommend that HEPES-BSS and the Trypsin a. Allow the trypsinization to continue until approximately 90% of
Neutralization Solution once stored at 4°C be used the cells are rounded up.
within one month. NOTE: Rounded up cells are spherical, have smooth edges and are refractile
or shiny. If the cells still have protruding nubs which are still attached to the
The following instructions are for a 25 cm2 flask. flask, they need more time to trypsinize. This entire process takes about
2 – 6 minutes, depending on cell type.
Adjust all volumes accordingly for other size flasks.
Technical Information
b. At this point, rap the flask against the palm of your hand to
Preparation release the majority of cells from the culture surface. If only a few
cells detach, you may not have let them trypsinize long enough.
Preparation for subculturing the first flask:
Clonetics®
Wait 30 seconds and rap again. If cells still do not detach, wait
1. Subculture the cells when they are 60 – 90% confluent and rap every 30 seconds thereafter.
and contain many mitotic figures throughout the NOTE: Do not try to get all cells to detach by rapping them severely. This
action may damage the cells.
flask.
2. For each 25 cm2 of cells to be subcultured: 7. After cells are released, neutralize the trypsin in
a. Thaw 2 ml of Trypsin/EDTA and allow to come to room temperature.
the flask with 4 ml of room temperature Trypsin
b. Allow 7 - 10 ml of HEPES Buffered Saline Solution (HEPES-BSS) to Neutralizing Solution. If the majority of cells do
come to room temperature. not detach within seven minutes, the trypsin is
c. Allow 4 ml of Trypsin Neutralizing Solution (TNS) to come to room either not warm enough or not active enough to
temperature.
release the cells. Harvest the culture vessel as
3. Remove growth medium from 4°C storage and allow described above, and either re-trypsinize with
to start warming to room temperature. fresh, warm Trypsin/EDTA Solution or rinse with
4. Prepare new culture vessels: Trypsin Neutralizing Solution and then add fresh,
a. Prepare 1 – 3 T-75 flasks. The number of flasks needed depends warm medium to the culture vessel and return to
upon confluence and total yield. Larger flasks may be used to an incubator until fresh trypsinization reagents are
save plasticware and time spent on subsequent subcultures.
Smaller flasks reduce the risk of losing a substantial part of your available.
culture.
b. As before, label each flask with the passage number, lot number, 8. Quickly transfer the detached cells to a sterile
cell type, and date. 15 ml centrifuge tube.
c. In a sterile field, carefully open the bottle and transfer growth
medium to new culture vessels by adding 1 ml growth medium 9. Rinse the flask with a final 2 ml of HEPES-BSS to
for every 5 cm2 surface area of the flask. collect residual cells, and add this rinse to the
Example: 15 ml growth medium for a 75 cm2 flask centrifuge tube.
d. If not using vented caps, loosen caps of flasks. Place the new
culture vessels into a 37°C humidified incubator with 5% CO2 10. Examine the harvested flask under the microscope
and equilibrate the flasks for at least 30 minutes. Subculture
one flask at a time. All flasks following the first flask will be
to make sure the harvest was successful by looking
subcultured following an optimization of this protocol (explained at the number of cells left behind. This should be
later in this procedure), based on calculated cell count, cell
viability, and seeding density.
less than 5%.
11. Centrifuge the harvested cells at 220 × g for 5 minutes
NOTE: Use only Clonetics® Trypsin/EDTA. The concentration of Trypsin/EDTA
from other suppliers may be 10X our concentration, which will detrimentally to pellet the cells.
effect Clonetics® Cells. a. Aspirate most of the supernatent, except for 100 µl to 200 µl.
b. Flick the centrifuge tube with your finger to loosen the pellet.
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Subculturing – Adherent Cell Types
Continued
12. Dilute the cells in 4 ml to 5 ml of growth medium 21. After mixing the diluted cells with a 5 ml pipette to
and note the total volume of the diluted cell ensure a uniform suspension, dispense the volume
suspension. of suspension calculated above into the prepared
NOTE: To obtain the best results from your cells, assess cell yield and subculture flasks.
viability with Trypan Blue.
22. After dispensing the cells, gently rock flask to
13. Count the cells with a hemacytometer or cell promote even distribution.
counter and calculate the total number of cells.
(See instructions above.) Make a note of your cell 23. If not using vented caps, loosen caps of flasks.
yield for later use. Place the new culture vessels into a 37°C humidified
NOTE: The cell suspension should contain between 250,000 to
incubator with 5% CO2.
1,000,000 cell/ml for greatest accuracy.
Technical Information
Example: 1,000,000 cells × 60% = 600,000 viable cells
Clonetics®
by using the equation below. The number of flasks
needed depends upon cell yield and seeding density.
Larger flasks may be used to save plasticware and
time spent on subsequent subcultures. Smaller
flasks reduce the risk of losing a substantial part of
your culture if contamination occurs.
The recommended seeding density could be 2,500 cells/cm2, 3,500 cells/cm2,
or 5,000 cells/cm2 for flasks and 10,000 cells /cm2 for well plates
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Subculturing into 96-well plates
Overview Procedure
A culture flask of normal human cells is harvested by 1. Follow the steps for subculture preparation and
trypsinization and subsequent trypsin inhibitor treatment. subculturing. Then follow steps 2 – 4 below.
The cells are centrifuged, resuspended in growth medium 2. Since the cells/ml calculation computed is per ml,
and counted. The desired number of cells is then added the cell concentration must be increased by 4 times
to wells of sterile 96-well tissue culture plates. The plates before seeding 96-well plates (to accommodate the
are incubated in a 37°C, 5% CO2 humidified incubator for 1 1:4 dilution when adding 250 µl of suspended cells
– 3 days to allow for cell adherence and growth. Seeding per well). When making the cell suspension, adjust
densities will vary somewhat with your experimental the cell concentration with growth medium.
requirements. A density of 10,000 cells/cm2 for multiwell 3. Transfer the diluted cell suspension to a sterile
plates is ideal. See the Cell Culture Instruction Sheet* for the reservoir. Using a multichannel (8 or 12 channel)
cell type you are using for specific information. pipette equipped with sterile pipette tips, add 250
*Available at www.lonza.com/research or contact Scientific Support. µl of the diluted cell suspension to each well of the
labeled 96-well flat bottom, tissue culture plate(s).
Required Materials: RESUSPEND THE CELL SUSPENSION OFTEN DURING THE SEEDING
1. T-25 flask of proliferating normal human cells between PROCEDURE TO ENSURE A UNIFORM NUMBER AND DISTRIBUTION OF
60% and 90% confluence CELLS INTO EACH WELL BY PIPETTING UP AND DOWN A FEW TIMES
BETWEEN EVERY OTHER DISPENSING.
2. 96-well flat bottom, tissue culture plates
3. 37°C humidified incubator with 5% CO2/95% air 4. Cover and incubate the plates for 1 – 3 days at
Technical Information
4. Laminar flow hood or other sterile environment 37°C/5% CO2. (Incubation periods exceeding 3 days
5. Adjustable multichannel pipette (8 or 12 channel) or are generally not recommended because of evaporation
Clonetics®
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Instructions for Cryopreservation
Technical Information
freezing canister.
6. Store cells at -70°C overnight.
Clonetics®
7. Within 12 – 24 hours, place in LN2 (-200°C) for
long-term storage. Cells will be compromised by
prolonged storage at -70°C.
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Improving Cell Yield and Viability During Subculture
Several factors, or a combination of factors, can contribute to low cell count and low cell viability. If cell yield or viability is
unsatisfactory, use the following information to increase the success rate of future cultures.
detached but the yield was low numerous mitotic figures throughout the flask
Clonetics®
If your cell viability is low (less than 50%), determine the possible cause(s) and solution(s) using the table below. Then subculture
one more flask applying the appropriate solution(s).
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Clonetics® Media
Clonetics® Media
human cell types. Results of years of media development
are available to you for use in your own research.
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Airway Cell Media Options
Airway cell media are serum-free media that have BEGM® BulletKit®
been optimized for the proliferation of airway cells.
— Best growth of NHBE - Normal Human Bronchial/
Each component of the basal medium and each growth
Tracheal Epithelial Cells in medium containing
supplement are carefully titered for optimal growth by
antimicrobials
Lonza’s R&D team. Lonza currently offers two media
choices for the growth of airway cells, allowing for desired
performance and formulation flexibility. When selecting a SAGM™ BulletKit®
medium to use, refer to specific media recommendations
— Superior growth for SAEC - Human Small Airway
or call Scientific Support for assistance.
Epithelial Cells
Product Information
Cat. No. Product Description
CC-3170 BEGM® BulletKit® Bronchial Epithelial Cell Growth Medium BulletKit®, Serum-free
CC-3171 BEBM® Basal Medium Bronchial Epithelial Cell Basal Medium, Serum-free
CC-4175 BEGM® SingleQuots® Kit Formulates BEBM® to BEGM®
BPE, 2 ml; Hydrocortisone, 0.5 ml;
hEGF, 0.5 ml; Epinephrine, 0.5 ml;
Transferrin, 0.5 ml; Insulin, 0.5 ml;
Clonetics® Media
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Endothelial Cell Media Options
Endothelial cell media are low serum media optimized EGM®-2 BulletKit®
for the proliferation of endothelial cells. Each component
— Refinements to basal medium and the growth factors
of the basal medium and each growth supplement is
carefully titered for optimal growth by Lonza’s R&D team. — Does not contain BBE
Lonza currently offers four Clonetics® Media for the growth — Final serum concentration is 2%
of endothelial cells allowing for desired performance
— Improved cell proliferation over EGM®
and formulation flexibility. When selecting a medium to
use, refer to specific medium recommendations or call — EGM®-2 can be used to grow all of Clonetics® Endothelial
Scientific Support for assistance. Cells except microvascular, coronary artery, umbilical
artery, and iliac artery
EGM® and EGM® BulletKit®
— Basal medium developed for normal human endothelial EGM®-2MV BulletKit®
cells in a low-serum environment — Developed for the enhanced growth of lung micro-
— EGM® is supplemented growth medium and includes vascular endothelial cells
an attached aliquot of Bovine Brain Extract (BBE) — Does not contain BBE
— EGM® BulletKit® includes basal medium with supple- — Final serum concentration increased to 5%
ments and growth factors in separate, frozen aliquots
— EGM®-2MV can be used to grow all Clonetics® Endothelial
— Final serum concentration is 2% Cells except HUVEC
Clonetics® Media
— EGM® can be used to grow all of Clonetics® Endothelial
Cells except microvascular, coronary artery, umbilical
artery, and iliac artery
EGM® MV BulletKit®
— Developed for microvascular and coronary artery
endothelial cells
— Same basal medium as in EGM®
— Final serum concentration is 5%
— EGM®-MV can be used to grow most Clonetics®
Endothelial Cells
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Endothelial Cell Media Options
Continued
Product Information
Cat. No. Product Description
CC-3125 EGM® MV BulletKit® Microvascular Endothelial Cell Growth Medium BulletKit® w/ 5% FBS
CC-3121 EBM® Basal Medium Endothelial Cell Basal Medium, Serum-free
CC-4143 EGM® MV SingleQuots® kit Formulates EBM® to EGM® MV
BBE, 2 ml; hEGF, 0.5 ml;
Hydrocortisone, 0.5 ml; FBS, 25 ml; GA-1000, 0.5 ml
CC-3202 EGM®-2 MV BulletKit® Microvascular Endothelial Cell Growth Medium-2 BulletKit® w/ 5% FBS
CC-3156 EBM®-2 Basal Medium-2 Endothelial Cell Basal Medium-2, Serum-free
CC-4147 EGM®-2 MV SingleQuots® Kit Formulates EBM®-2 to EGM®-2 MV
hEGF, 0.5 ml; Hydrocortisone, 0.2 ml; FBS, 25 ml;
VEGF, 0.5 ml; hFGF-B, 2 ml; R3-IGF-1, 0.5 ml;
Ascorbic Acid, 0.5 ml; GA-1000, 0.5 ml
(No BBE)
CC-3024 EGM® Complete Medium Endothelial Cell Growth Medium w/ 2% FBS
CC-3124 EGM® BulletKit® Endothelial Cell Growth Medium BulletKit® w/ 2% FBS
CC-3121 EBM® Basal Medium Endothelial Cell Basal Medium, Serum-free
CC-3129 EBM®-PRF EBM® w/o Phenol Red
CC-4133 EGM® SingleQuots® Kit Formulates EBM® to EGM®
BBE, 2 ml; hEGF, 0.5 ml;
Hydrocortisone, 0.5 ml; FBS, 10 ml; GA-1000, 0.5 ml
CC-3162 EGM®-2 BulletKit® Endothelial Cell Growth Medium-2 BulletKit® w/ 2% FBS
CC-3156 EBM®-2 Basal Medium-2 Endothelial Cell Basal Medium-2, Serum-free
CC-4176 EGM®-2 SingleQuots® Kit Formulates EBM®-2 to EGM®-2
hEGF, 0.5 ml; Hydrocortisone, 0.2 ml; FBS, 10 ml;
Clonetics® Media
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Fibroblast Cell Media Options
Fibroblast media has been optimized for the proliferation of FGM® BulletKit®
fibroblasts. Each component of the basal medium and each
— FGM® is a defined medium system and does not contain
growth supplement is carefully titered for optimal growth
serum
by Lonza’s R&D team. Lonza currently offers two media
choices for the growth of fibroblasts, allowing for desired
performance and formulation flexibility. When selecting a FGM®-2 BulletKit®
medium to use, refer to specific media recommendations
— Contains a vial of FBS for a final serum concentration
or call Scientific Support for assistance.
of 2%
Product Information
Cat. No. Product Description
CC-3132 FGM®-2 BulletKit® Fibroblast Growth Medium BulletKit®-2, w/ 2% FBS
CC-3131 FBM® Basal Medium Fibroblast Basal Medium
CC-4126 FGM® SingleQuots® Kit Formulates FBM® to FGM-2
hFGF-B, 0.5 ml; Insulin, 0.5 ml FBS, 10 ml;
GA-1000, 0.5 ml
CC-3130 FGM® BulletKit® Fibroblast Growth Medium BulletKit®, Defined
CC-3131 FBM® Basal Medium Fibroblast Basal Medium
CC-4134 FGM® SingleQuots® Kit Formulates FBM® to FGM
hFGF-B, 0.5 ml; Insulin, 0.5 ml; GA-1000, 0.5 ml
Clonetics® Media
Hepatocyte Media
Product Information
Cat. No. Product Description
CC-3198 HCM™ BulletKit® Hepatocyte Culture Medium, Phenol red-free
CC-3199 HBM™ Basal Medium Hepatocyte Basal Medium, Phenol red-free, Serum-free
CC-4182 HCM™ SingleQuots® Kit Formulates HBM™ to HCM™
hEGF, 0.5 ml; Transferrin 0.5 ml; Hydrocortisone, 0.5 ml;
BSA, 10.0 ml; Ascorbic Acid 0.5 ml; GA-1000, 0.5 ml, Insulin, 0.5 ml
CC-3197 HMM™ Medium HMM™, Hepatocyte Maintenance Medium, Phenol red-free, Serum-free
CC-4192 HMM™ SingleQuots® Kit HMM™ SingleQuots®, required supplements for use with HMM™
to provide optimal maintenance of cells, Insulin;
0.5 ml Dexamethasone, 0.5 ml; GA-1000, 0.5 ml
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Keratinocyte Cell Media Options
Keratinocyte media has been optimized for the proliferation KGM®-2 BulletKit®
of keratinocytes. Each component of the basal medium
— Refinements to the basal medium and the growth
and each growth supplement is carefully titered for optimal
factors
growth by Lonza’s R&D team. Lonza currently offers three
media choices for the growth of keratinocytes, allowing — Grows normal human keratinocytes up to 20% faster
for desired performance and formulation flexibility. and allows for additional population doublings when
When selecting a medium to use, refer to specific media compared to KGM® and other commercially available
recommendations in the cell systems sections or call your keratinocyte media
Scientific Support for assistance. — KGM®-2 can be used to grow all Clonetics® NHEK Normal
Human Epidermal Keratinocytes
KGM® and KGM® BulletKit®
KGM®-CD
— Optimized for Clonetics® NHEK – Normal Human
Epidermal Keratinocytes in a serum-free environment — Supports isolation and proliferation of primary human
— KGM® includes supplemented medium and an attached keratinocytes in culture
aliquot of Bovine Pituitary Extract (BPE) — Chemically defined – No serum and no animal or plant
— KGM® BulletKit® includes basal medium with all extracts
supplements and growth factors in separate, frozen — Minimized variable experimental results due to
aliquots unknown effects of animal or plant-derived
Clonetics® Media
Product Information
Cat. No. Product Description
CC-3107 KGM®-2 BulletKit® Keratinocyte Growth Medium-2 BulletKit®, Serum-free
CC-3103 KBM®-2 Basal Medium-2 Keratinocyte Basal Medium-2
CC-3108 KGM®-2 w/o Ca++ BulletKit® Keratinocyte Growth Medium-2 BulletKit®, Calcium-Free, Serum-free
CC-3158 KBM®-2 w/o Ca++ Keratinocyte Basal Medium-2, Calcium-Free, Serum-free
CC-4152 KGM®-2 SingleQuots® Kit Formulates KBM®-2 to KGM®-2
BPE, 2 ml; hEGF, 0.5 ml; Insulin, 0.5 ml
Hydrocortisone, 0.5 ml; Epinephrine, 0.5 ml; Transferrin, 0.5 ml;
GA-1000, 0.5 ml
CC-3001 KGM® Complete Medium Keratinocyte Growth Medium, Serum-free
CC-3111 KGM® BulletKit® Keratinocyte Growth Medium BulletKit®, Serum-free
CC-3101 KBM® Basal Medium Keratinocyte Basal Medium, Serum-free
CC-3112 KGM® BulletKit® w/o Ca++ Keratinocyte Growth Medium BulletKit®, Calcium-free, Serum-free
CC-3104 KBM® w/o Ca++ Keratinocyte Basal Medium, Calcium-free, Serum-free
CC-4131 KGM® SingleQuots® Kit Formulates KBM® to KGM®
BPE, 2 ml; hEGF, 0.5 ml; Insulin, 0.5 ml;
Hydrocortisone, 0.5 ml; GA-1000, 0.5 ml
CC-4455 KGM®-CD BulletKit® Keratinocyte Growth Media – Serum-free, Non-animal origin components
CC-3255 KBM®-CD Basal Medium Keratinocyte Basal Media – Chemically Defined
CC-4456 KGM®-CD SingleQuots® Kit Formulates KBM®-CD to KGM®-CD
Recombinant human insulin 1 ml; growth supplement, 5 ml
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Mammary Epithelial Cell Media, Serum-free
Product Information
Cat. No. Product Description
CC-3051 MEGM® Complete Medium Mammary Epithelial Cell Growth Medium, Serum-free, complete medium
CC-3150 MEGM® BulletKit® Mammary Epithelial Cell Growth Medium BulletKit®, Serum-free
CC-3151 MEBM® Basal Medium Mammary Epithelial Cell Basal Medium, Serum-free
CC-3153 MEBM®-PF free Mammary Epithelial Cell Basal Medium Phenol Red Free, Serum-free
CC-3152 MEBM®-Bicarb free Mammary Epithelial Cell Basal Medium Sodium Bicarbonate Free, Serum-free
CC-4136 MEGM® SingleQuots® Kit Formulates MEBM® to MEGM®
BPE, 2 ml; Hydrocortisone, 0.5 ml;
hEGF, 0.5 ml; Insulin, 0.5 ml; GA-1000, 0.5 ml
Clonetics® Media
Melanocytes in culture. This medium system has been — Melanocyte Growth Media has been qualified and
shown to deliver superior results as compared to other tested together to provide optimum performance
existing commercial media systems. Melanocytes in culture
maintain >90% functionality based on the conversion of — The media system is offered as BulletKit® (basal
L-dopa to dopa-melanin. The Melanocyte Media system medium and separately packaged growth factors) to
also allows for normal morphology and proliferative capacity allow for flexibility with your research project
after recovery from cryopreservation and throughout
serial passaging. — Adult melanocytes also require the addition of Et-3,
sold separately
Product Information
Cat. No. Product Description
CC-3249 MGM® BulletKit® Melanocyte Growth Medium
CC-3250 MBM®-4 Basal Medium-4 Melanocyte Basal Medium, Serum-free
CC-4435 MGM®-4 SingleQuots® Kit Formulates MBM®-4 to MGM®-4
CaCl2, 1.0 ml; hFGF-B, 1.0 ml; PMA, 0.5 ml; rhInsulin, 1.0 ml;
Hydrocortisone, 0.5 ml; BPE, 2.0 ml; FBS, 2.5 ml;
Gentamicin/Amphotericin B, 0.5 ml
Product Information
Cat. No. Product Description
CC-3186 AGM™ BulletKit® Astrocyte Growth Medium BulletKit®, w/3.0% FBS
CC-3187 ABM™ Basal Medium Astrocyte Basal Medium, Serum-free, without L-glutamine
CC-4123 AGM™ SingleQuots® Kit Formulates ABM™, to AGM™
Insulin, 1.25 ml; rhEGF, 0.5 ml;
FBS, 15.0 ml; Ascorbic Acid, 0.5 ml;
L-glutamine, 5.0 ml; GA-1000, 0.5 ml
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Prostate Epithelial Cell Medium, Serum-free
Product Information
Cat. No. Product Description
CC-3166 PrEGM™ BulletKit® Prostate Epithelial Cell Growth Medium BulletKit®, Serum-free
CC-3165 PrEBM™ Basal Medium Prostate Epithelial Cell Basal Medium, Serum-free
CC-4177 PrEGM™ SingleQuots® Kit Formulates PrEBM® to PrEGM®
BPE, 2.0 ml; Hydrocortisone, 0.5 ml; hEGF, 0.5 ml;
Epinephrine, 0.5 ml; Transferrin, 0.5 ml; Insulin, 0.5 ml;
Retinoic Acid, 0.5 ml; Triiodothyronine, 0.5 ml;
GA-1000, 0.5 ml
Product Information
Clonetics® Media
Product Information
Cat. No. Product Description
CC-4461 PNGM™ BulletKit® Primary Neuron Growth Medium BulletKit®
CC-3256 PNBM™ Basal Medium Primary Neuron Basal Medium
CC-4462 PNGM™ SingleQuots® Kit Formulates PNBM to PNGM™
NSF-1, 4 ml; L-glutamine, 2 ml; GA-1000, 0.2 ml
CC-4512 PNGM™-A BulletKit® Primary Neuron Growth Media-Adult
CC-4511 PNGM™-A SingleQuots® Kit Formulates PNBM to PNGM™-Adult
NSF-1, 4 ml; L-glutamine, 2 ml; Pen/Strep, 2 ml
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Skeletal Cell Media
Product Information
Cat. No. Product Description
CC-3207 OGM™ BulletKit® Osteoblast Growth Medium BulletKit®, w/ 10% FBS
CC-3208 OGM™ Basal Medium Osteoblast Cell Basal Medium, Serum-free
CC-4193 OGM™ SingleQuots® Kit Formulates OBM™ to OGM™
FBS, 50 ml; Ascorbic Acid, 0.5 ml; GA-1000, 0.5 ml
CC-4194 OGM™ Differentiation SingleQuots® Kit Induces osteoblast differentiation and bone mineralization.
Hydrocortisone-21-Hemisuccinate, 0.5 ml;
β-Glycerophosphate, 5.0 ml
CC-3216 CGM™ BulletKit® Chondrocyte Growth Medium BulletKit®
CC-3217 CBM Basal Medium Chondrocyte Basal Medium
CC-4409 CGM™ SingleQuots® Kit Formulates CBM to CGM™
R3-IGF-1, 1.0 ml; bFGF, 2.5 ml; Insulin, 1.0 ml;
Transferrin, 0.5 ml; FBS, 25 ml;
Gentamicin/Amphotericin-B, 0.5 ml
CC-3225 CDM™ BulletKit® Chondrocyte Differentiation Medium BulletKit®, Serum-free
CC-3226 CDM™ Basal Medium Chondrocyte Differentiation Basal Medium
CC-4408 CDM™ SingleQuots® Kit Formulates CDM™ Basal Medium to CDM™ Differentiation Medium
TGF-β, 1.25 ml; R3-IGF-1, 0.5 ml; Insulin, 0.5 ml;
Transferrin, 0.5 ml; FBS, 12.5 ml; GA-1000, 0.25 ml
Clonetics® Media
Skeletal Muscle Cell Media
Product Information
Cat. No. Product Description
CC-3160 SkGM® BulletKit® Skeletal Muscle Growth Medium BulletKit®, Serum-free
CC-3161 SkBM® Basal Medium Skeletal Muscle Basal Medium, Serum-free
CC-4139 SkGM® SingleQuots® Kit Formulates SKBM® to SKGM®
hEGF, 0.5 ml; Insulin, 5 ml;
BSA, 5 ml; Fetuin, 5 ml;
Dexamethasone, 0.5 ml; GA-1000, 0.5 ml
CC-3245 SkGM®-2 BulletKit® Skeletal Muscle Myoblast Growth Medium-2 BulletKit®, with 10% FBS
CC-3246 SKBM®-2 Basal Medium-2 Skeletal Muscle Myoblast Basal Medium-2, Serum-free
no L-glutamine
CC-3244 SkGM®-2 SingleQuots® Kit Formulates SKBM®-2 to SKGM®-2
FBS, 50.0 ml; GA-1000, 0.5 ml; rhEGF, 0.5 ml;
Dexamethasone, 0.5 ml; L-glutamine,10.0 ml
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Smooth Muscle Cell Medium
Smooth muscle medium has been optimized for the SmGM®-2 BulletKit®
proliferation of smooth muscle cells. Each component
— SmGM®-2 BulletKit® was developed for better cell
of the basal medium and each growth supplement is
morphology and proliferation
carefully titered for optimal growth by Lonza’s R&D team.
— Final serum concentration is 5%
— SmGM®-2 can be used to grow all smooth muscle cells
Product Information
Cat. No. Product Description
CC-3182 SmGM®-2 BulletKit® Smooth Muscle Growth Medium-2 BulletKit® w/ 5% FBS
CC-3181 SmBM® Basal Medium Smooth Muscle Basal Medium, Serum-free
CC-4149 SmGM®-2 SingleQuots® Kit Formulates SmBM® to SmGM®-2
hEGF, 0.5 ml; Insulin, 0.5 ml;
hFGF- B, 1 ml; FBS, 25 ml; GA-1000, 0. 5 ml
Clonetics® Media
Product Information
Cat. No. Product Description
CC-3205 SCGM™ BulletKit® Stromal Cell Growth Medium BulletKit®, w/ 5% FBS
CC-3204 SCBM™ Basal Medium Stromal Basal Medium, w/o phenol red, Serum-free
CC-4181 SCGM™ SingleQuots® Kit Formulates SCBM™ to SCGM™
hFGF-B, 0.5 ml; Insulin 0.5 ml;
FBS, 25 ml; GA-1000, 0.5 ml
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Poietics® FAQs
Media
Q. What is a BulletKit®? Q. I don’t see the specific cell type I want in your
catalog or on your website, do you do custom
A. A BulletKit® contains basal medium and
SingleQuots® Kit of supplements and growth factors. isolations?
In most cases, it is possible to purchase each of A. Yes, Lonza offers custom isolations and formats
these components separately. Once the basal for various types of non-catalog cell products. See
medium is supplemented with the SingleQuots® page 79 & 98 for additional information.
Kit, it becomes a complete growth or differentiation
medium. Q. Can I expand the mononuclear cells, CD34+
progenitors, or the CD133+ cells without
Q. What are SingleQuots® Supplements and Growth
differentiating them?
Factors?
No, the cells will proliferate and differentiate
A. SingleQuots® Supplement and Growth Factors
simultaneously upon being placed in culture.
contains several vials of growth factors,
Varying the cytokine cocktail can influence the
supplements and antibiotics in premeasured
differentiation and proliferation of the cells. Please
aliquots designed to be added to a bottle of
contact Scientific Support for more information.
basal medium. Serum will be provided in the
SingleQuots® Kit if it is to be added to the Q. Can I expand the human mesenchymal stem
basal medium. The concentrations of these growth cells prior to differentiating them?
factors are proprietary.
A. These cells are frozen in 2nd passage and we
Q. What media should I use for Poietics® Cells? recommend they are used before passage 5.
Poietics® FAQs
A. Information regarding specific media selections Q. Can I expand the preadipocytes prior to differ-
can be found in the Technical Data Sheet or the entiating them?
Instructions. Please contact Scientific Support for
more information. A. The subcutaneous cells can be passaged twice
prior to differentiation. The visceral cells can be
Q. What is the difference between the different cell passaged once prior to being differentiated.
sources: bone marrow, cord blood, or fetal liver?
Q. Can I get subcutaneous and visceral preadipocytes
A. Bone Marrow: provides a hematopoietic micro- from the same donor?
environment and the most number of different
donors. A. Yes, please contact Scientific Support for more
Cord Blood: more naïve cells useful in transplant information.
and stem cell research.
Fetal Liver: best for developmental studies and they
are highly proliferative.
Q. Are the fresh cells you provide equivalent to their
cryopreserved counterparts?
A. Yes, the cells perform equivalently by all
measures we have tested. Some cells do die in the
cryopreservation and thawing processes, but it
does not seem to be selective to a particular cell
type. We put additional cells in each vial to account
for this loss.
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Procedure for Thawing CD34+ and Precursor Cells
1. Warm medium containing 10% FBS or 1% BSA. For 9. Slowly bring the volume up to fill the tube by adding
mononuclear cells and hematopoietic progenitors, 1 ml to 2 ml volumes of medium while gently
DNase I (20 U/ml) should also be added.* swirling after each addition of medium.
2. Quickly thaw the vial of frozen cells in a 37°C water 10. Centrifuge the cell suspension at 200 × g at room
bath. Wipe the outside of the vial with 70% ethanol. temperature for 15 minutes.
3. Aseptically transfer a maximum of 2 ml of cell 11. Carefully remove by pipette all but 2 ml of the wash.
suspension to a 50 ml conical tube. For one million Gently resuspend the cell pellet in the remaining 2
cells or less, use a 15 ml conical tube. ml of medium and count. If cell count is lower than
4. Rinse the vial with 1 ml of medium. Add the rinse expected, centrifuge the wash saved in step 8 at a
dropwise to the cells while gently swirling the tube higher speed, count and combine if necessary.
(≈1 minute). 12. Rest the cells for 1 hour at 37°C and 5% CO2. Count
5. Slowly add enough medium dropwise to the cells the cells a second time. The cells are ready to be
until the total volume is 5 ml, while gently swirling put in culture.
*For the addition of DNase, prepare 20 ml of medium
after each addition of several drops of medium containing 10% FBS and 20 U/ml of DNase I (Sigma D 4513).
(≈3 minutes). Proceed as above, using the DNase-containing medium to
dilute the cells. Centrifuge the cells and continue with step #8.
6. Slowly bring the volume up to fill the tube by adding
1 ml to 2 ml volumes of medium dropwise, while
Technical Information
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Human Mesenchymal Stem Cell Media
Product Information
Cat. No. Product Description
PT-3001 MSCGM™ BulletKit® Mesenchymal Stem Cell Growth Medium BulletKit®
PT-3238 MSCGM™ Basal Medium Mesenchymal Stem Cell Basal Medium
PT-4105 MSCGM™ SingleQuots® Kit Formulates MSCBM to MSCGM™ Growth Medium
MCGS, 50 ml; L-glutamine, 10 ml; GA-1000, 0.5 ml
PT-3002 hMSC Differentiation Kit– Osteogenic Mesenchymal Stem Cell Differentiation Kit – Osteogenic
PT-3924 hMSCBM – Osteogenic Basal Medium Mesenchymal Stem Cell Basal Medium – Osteogenic
PT-4120 hMSC SingleQuots® Kit – Osteogenic Formulates Osteogenic Basal Medium to Osteogenic Differentiation Medium
Dexamethasone, 1 ml; β-Glycerophosphate, 2 ml; Ascorbate, 1 ml;
Penicillin /Streptomycin, 2 ml; MCGS, 20 ml; L-glutamine, 4 ml
PT-3003 hMSC Differentiation Kit – Chondrogenic Mesenchymal Stem Cell Differentiation Kit – Chondrogenic
PT-3925 hMSCBM – Chondrogenic Basal Medium Mesenchymal Stem Cell Basal Medium – Chondrogenic
PT-4121 hMSC SingleQuots® Kit – Chondrogenic Formulates Chondrogenic Basal Medium to Chondrogenic Differentiation Medium
ITS+, 2 ml; Sodium Pyruvate, 2 ml; Proline, 2 ml; Dexamethasone, 1 ml;
Ascorbate, 2 ml; GA-1000, 0.2 ml; L-glutamine, 4 ml
PT-4124 TGF-β3 Required component sold separately
PT-3004 hMSC Differentiation Kit – Adipogenic Mesenchymal Stem Cell Differentiation Kit – Adipogenic
PT-3102A hMSC Adipogenic Maintenance Medium Mesenchymal Stem Cell Maintenance Medium – Adipogenic
PT-3102B hMSC Adipogenic Induction Medium Mesenchymal Stem Cell Induction Medium – Adipogenic
PT-4122 hMSC Maintenance SingleQuots® Kit – Adipogenic Formulates Adipogenic MM to Adipogenic Differentiation Medium
rhInsulin, 2 ml; GA-1000, 0.2 ml; MCGS, 20 ml; L-glutamine, 4 ml
Poietics® Media
PT-4135 hMSC Induction SingleQuots® Kit – Adipogenic Formulates Adipogenic IM to Adipogenic Differentiation Medium
Indomethacin, 0.4 ml; IBMX, 0.2 ml; rhInsulin, 2 ml; Dexamethasone, 1 ml;
GA-1000, 0.2 ml; MCGS, 20 ml; L-glutamine, 4 ml
Product Information
Cat. No. Product Description
PT-8002 PGM™-2 BulletKit® Preadipocyte Growth Medium-2 BulletKit®
PT-8202 PBM-2 Basal Medium-2 Preadipocyte Basal Medium-2
PT-9502 PGM™-2 SingleQuots® Kit Formulates PBM-2 to PGM™-2 FBS (10%), 50 ml; L-Glutamine, 5 ml; GA-
1000, 0.2 ml, 5 ml; rhInsulin, 2 ml; Dexamethasone, 0.2 ml; Indomethacin,
0.4 ml; IBMX, 0.2 ml
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Osteoclast Growth Medium
Product Information
Cat. No. Product Description
PT-8001 Osteoclast Growth Medium BulletKit® Osteoclast Growth Medium BulletKit®
PT-8201 OBM™ Osteoclast Basal Medium
PT-9501 Osteoclast Growth Medium SingleQuots® Kit Formulates OBM™ to Osteoclast Growth Medium FBS (10%), 10 ml;
L-glutamine, 1 ml; Penicillin/Streptomycin, 1 ml; M-CSF, 0.1 ml;
Soluble RANK Ligand, 2 μg
Product Information
Cat. No. Product Description
CC-3209 NPMM™ BulletKit® Neural Progenitor Maintenance Medium BulletKit®
Poietics® Media
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Amaxa® Nucleofection®
and Transfection
Chapter 2
Amaxa® Nucleofection® and Transfection
and Transfection
Nucleofection®
Transfection Reagents
HiFect® Transfection Reagent 191
Transport™ Protein Delivery Reagent 202
PrimeFect™ Transfection Reagent 203 & 204
FAQs Transport Reagent 205
Vectors
pmaxFP® Fluorescent Protein Expression Vectors 192
pmaxFP® Vector Backbone Information 197
pmaxFP®-Green Vectors 198
pmaxFP®-Yellow Vectors 199
pmaxFP®-Red Vectors 200
pmaxCloning™ Vector 201
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Amaxa® Nucleofection® and Transfection
and Transfection
Nucleofection®
Selecting the Ideal Technology and Product
for your Needs
Lonza provides comprehensive information and protocols
regarding the introduction of many bio-molecules and
particles. This information and more, is available online
at www.lonza.com/celldatabase or through Scientific
Support. Nucleofector® Devices and Kits are available
in both single sample and 96-well formats to suit your
individual throughput needs and to ensure that you have
continuity of transfection method as your processing
capacity increases over time. The selection chart below,
and others hosted on the Lonza website, are designed to
help you quickly focus your transfection methodologies.
Identify the right methodology based on your cell type and substrate
Substrate
DNA RNA Protein Other
Plasmid DNA, cDNAs, siRNA, shRNA, Peptide, Protein Morpholinos, pNAs, etc.
Cell type Gene constructs miRNA
Primary cells Nucleofector® Device Nucleofector® Device Nucleofector® Device Nucleofector® Device
96-well Shuttle® System 96-well Shuttle® System 96-well Shuttle® System 96-well Shuttle® System
Standard cell lines HiFect® Reagent HiFect® Reagent Nucleofector® Device Nucleofector® Device
Nucleofector® Device Nucleofector® Device 96-well Shuttle® System 96-well Shuttle® System
96-well Shuttle® System 96-well Shuttle® System
Contact Lonza Scientific Support to review your selection and discover more about the solution that fits your needs!
Scientific Support
Europe/World USA
– Phone: +49(0)221-99199-400 – Phone: 888-632-9110
– Fax: +49(0)221-99199-499 – Fax: (888) 632-9112
– Email: scientific.support.eu@lonza.com – Email: scientific.support@lonza.com
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Amaxa® Nucleofector® Technology
Continued
Nucleofector®
Technology
Amaxa® Nucleofector® Technology and into the nucleus. Transfected cells retain excellent
viability and the function of intracellular systems is
The application of systems biology and multidisciplinary
highly conserved. Whatever your application, Amaxa®
approaches require that cells and model systems display
Transfection Specialists are available to assist you in
’in-vivo-like’ cellular functionality. This means that the
rapidly optimizing your new application.
future of cell transfection is in using primary cell types, and
that transfecting these physiologically relevant cell types
One technology – the broadest choice of cell types
presents researchers with a series of technical challenges.
Using the Nucleofector® Technology, cell lines, as well
The most physiologically relevant cell types are typically
as primary cells, can be reliably transfected at high
the most difficult to transfect using traditional methods. In
efficiency. Delivery of nucleic acid substrates directly into
contrast, when using relevant cell lines as model systems,
both the nucleus and cytoplasm ensures transfection
the critical issues are to achieve reproducibly efficient
efficiencies of up to 99% in both cell lines and primary
transfection with high levels of viability while matching
cells. More than 200 ready-to-use Amaxa® Optimized
throughput capability with the number of transfections
Protocols contain cell-type specific guidance and Lonza's
required at each project phase - from proof of concept,
Amaxa® Cell Database contains user-developed methods
through scale-up and screening-like applications.
and data for more than 1300 cell types.
Plasmid Pre-
miRNA
shRNA
System Biology
DNA Antisense
Oligos
Functional
Antibodies Genomics
Peptide siRNA
Protein
C D RNA
Protein
Small miRNA
Molecules
Drug mRNA
Candidates
Fluoresent
conjugates
Drug Discovery
Normal human dermal fibroblasts - neonatal were transfected with 2.5 µg Substrate flexibility – explore the limits of your
TMR-labeled plasmid DNA encoding eGFP. After 2 hours, cells were fixed
with 3.5% PFA and analyzed by confocal microscopy. TMR label is shown in
imagination
(A), GFP fluorescence in (B), DAPI nuclear staining in (C) and a merge of all The flexibility that Nucleofection® allows in planning
three fluorescent labels in (D).
experimental projects stems from Lonza's proven
capability to transfect the widest range of substrate
Amaxa® Nucleofection® – your unique advantage molecules into the widest range of cell types. Imagine
Amaxa® Nucleofection® is a proprietary technology the flexibility to first complement a knock-out mutant by
based on the momentary creation of small pores in transfecting a DNA vector, then to explore the regulation
cell membranes by applying an electrical pulse. The of the gene(s) of interest using multiple or single shRNA,
comprehensive way in which Nucleofector® Pulse siRNA, or miRNA substrates using the same Nucleofector®
Conditions and cell-type specific solutions are developed Kit and conditions.
ensures that nucleic acid substrates are delivered not only
Reflecting the focus of molecular biologists, the flexibility
to the cytoplasm, but also through the nuclear membrane
of Nucleofection® now extends beyond nucleic acid
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Amaxa® Nucleofector® Technology
Continued
Nucleofector®
Technology
substrates, to include peptides, proteins, and antibodies. The Nucleofector® II Device and 96-well Shuttle® System
This allows yet further characterization and validation of Both the Nucleofector® Device and 96-well Shuttle® System
the cellular functions that interest your research group, deliver unique electrical parameters that are different from
e.g., allowing researchers to identify and characterize other commercially available electroporation instruments.
related signaling or protein modification pathways Electrical settings are pre-programmed for each optimized
using inhibitory peptides, before tracking active protein cell type and can be selected using the Nucleofector®
trafficking, or localization of active target molecules using Device or the PC-based software controlling the
antibody-conjugated nano-fluorophores or quantum-dots. 96-well Shuttle® Device.
Two platforms
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Amaxa® Nucleofector® Technology
Continued
Nucleofector®
Technology
100
50
40
30
20
10
0 1 3 5 7 9 1 13 15 1
Gene silencing in primary neurons by transfection of an shRNA vector – Quantitive down-regulation of CDC10 protein
A B C D E
2 100
40
Calnexin → 20
Rat hippocampal neurons (E17) were transfected with the shRNA vector pSUPERIOR targeting CDC10 using the 96-well Shuttle® System. A and B: Efficient
Nucleofection® of pSUPERIOR is shown by eGFP expression after 1 day in vitro. C: Immunostaining of CDC10 (red fluorescence) shows reduced endogenous
CDC10 protein levels in transfected neurons (green) after 4 days in vitro compared to untransfected cells (red). Western blot analysis (D) and quantification (E)
of CDC10 down regulation.
(Data courtesy of Prof. Kiebler, Medical University of Vienna, Vienna, Austria.)
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Amaxa® Nucleofector® Technology
Continued
Nucleofector®
Technology
The Ideal Tool for Transfection of Cell Lines and Primary Cells
Cell Lines
Broad cell line coverage – From standard to difficult-to-transfect cells, including suspension cells
– More than 100 ready-to-use protocols
– Fast and easy optimization
Fast experiments – Expression within hours – from transfection to analysis in one day
Primary Cells
High efficiencies – Up to 90% efficiency with plasmid DNA
– Up to 99% efficiency with siRNA duplexes
No optimization – Ready-to-use kits for a multitude of cell types, e.g. neurons and stem cells
Reproducible results – Optimized Protocols for each cell type with details on cell isolation
and culture
Unique advantages – Direct transport of nucleic acids into the nucleus thus enabling transfection
even of non-dividing cells
Contact Lonza today regarding your unique application or to arrange to test the Nucleofector® Technology with your cells
and in your lab.
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Nucleofector® II Device
Nucleofector®
Technology
– Program delivery unit for 96-well Shuttle® System Protection Class IP 20, EN 61010-1, UL 61010A-1
Technical Information
Clonetics®
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96-well Shuttle® Device
96-well Shuttle®
Device
The 96-well Shuttle® Device is a high throughput extension Ordering Information
to the Nucleofector® II Device. The complete Nucleofector®
Cat. No. Price
96-well Shuttle® System consists of three components,
the 96-well Shuttle® Device, the Nucleofector® II Device 96-well Shuttle® Device
and a laptop computer. The Nucleofector® II Device serves AAM-1001
as the program delivery unit. The 96-well Shuttle® Device
including:
is the contacting unit which mediates the transfer of Laptop and 96-well Shuttle® Software
the respective 96-well program to a specific well of the The Nucleofector® II Device must be purchased independently
96-well Nucleocuvette® Plate. The interaction between
Nucleofector® II Device and Nucleofector® 96-well Shuttle®
Device is controlled via the 96-well Shuttle® Software
installed on the laptop.
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Nucleofector® Device Extended Guarantee and Service Contracts
Nucleofector® Device
– 1 or 2 additional years of guarantee beyond the basic Service Contract 96-well Shuttle® Device Gold
device guarantee of 1 year AWB-2002
– Diagnosis and repair of your 96-well Shuttle® Device
including all replacement parts
– Costs for return shipments of repaired device
– Replacement of 96-well Shuttle® Device within
5 business days*
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96-well Shuttle® Automation Package
Automation Package
96-well Shuttle®
Lonza’s 96-well Shuttle® Automation Package is the first Ordering Information
high throughput transfection device suitable for the
Cat. No. Size Price
transfection of difficult-to-transfect cell lines and primary
cells. For a complete automation of the Nucleofection® 96-well Shuttle® Automation Package
Process, the Nucleofector® 96-well Shuttle® System has SBA-1001
to be integrated into an automated environment providing
Software and Documentation:
liquid handling and plate handling capabilities such as – CD containing the 96-well Shuttle® Automation Software
Liquid Handling platforms from Tecan, Beckman Coulter, – Manual with detailed interface documentation
Hamilton, or other suppliers. For the integration, additional Support:
software is necessary to control the Nucleofection® – Support to LHS supplier during integration process
– On-site test support
Process via the Liquid Handling Software (LHS). – On-site installation support
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96-well Shuttle® Automation Package
Automation Package
96-well Shuttle®
Example for Fully Automated Cell Transfection with Tecan and Lonza’s
New Combined Platform
Combining the Nucleofector® 96-well Shuttle® System and a Tecan® Freedom EVO®
liquid handling workstation allows fully automated, precise and efficient transfection
of difficult-to-transfect cell lines and primary cells. The integrated system is ideal
for large-scale studies that involve high throughput transfection stages such as
RNAi-based screening for target identification and validation, or screening of cDNA
libraries. The present platform will be used for siRNA experiments based on human T
lymphocytes and Jurkat cells using focused siRNA libraries.
Integration and Interaction
The 96-well Shuttle® System is positioned on the Freedom EVO® Worktable using
a special adapter plate. Nucleocuvette® Plates, which are first prepared using the
eight channel liquid handling arm or the multichannel option with stainless steel or
disposable tips, can be loaded on the 96-well Shuttle® System and retrieved after the
Nucleofection® Process using the robotic manipulator. The 96-well Shuttle® Software is
controlled by Tecan’s Freedom EVOware® Robotics Control Software using the interface
provided by Lonza.
Rapid, Precise and Reliable Liquid Handling
The freely configurable Freedom EVO® Workstation can accommodate a wide range
of modules, including the robotic manipulator arm to transfer plates to and from
the 96-well Shuttle® System. Integrating the 96-well Shuttle® System and a robotic
incubator with the Freedom EVO® Workstation completely substitutes all manual steps
for cell preparation and transfection, generating consistent reproducible results. The
platform can process a 96-well Nucleocuvette® Plate in just minutes, depending on
the protocol used.
The Freedom EVO® Workstation is available in different sizes and with a variety of
options, including:
■ Liquid handling arm with fixed or disposable (filter) tips
■ Multichannel pipettors for plating cells or direct transfer of compounds
from plate to plate
■ Robotic CO2 incubator for automated cell storage, delivery and incubation
■ Option for harvesting of adherent cells from robotic-friendly cell culture flasks
■ Cooled or heated carriers for media, nucleic acids and Nucleofector® Solution
■ Integrated thermocyclers for PCR applications
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96-well Shuttle® Automation Package
Automation Package
96-well Shuttle®
Process Control
Using the Lonza interface included in the 96-well Shuttle® Automation Package the
entire Nucleofection® Process is controlled by the Freedom EVOware® Software, which
allows for the implementation of individual and specific plate preparation protocols.
Nucleofection® conditions for the 96-well Shuttle® System are defined in the Lonza
software and the resulting parameter files are uploaded from Freedom EVOware®
Software and executed on the 96-well Shuttle® System.
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List of Nucleofector® Kits for Primary Cells
Nucleofector® Kits
Blood Cells
B cell, human NF 36% 84 – 92%
B cell, human 96 28% 70%
B cell, mouse, stimulated NF 49 – 59% 27 – 47%
B cell, mouse, stimulated 96 55 – 56% 41 – 87%
CD34+ cell, human NF 71%
Dendritic cell, human NF 49% 71%
Dendritic cell, mouse, immature, BALB/c NF 58% 62%
Dendritic cell, mouse, immature, C57BL/6 NF 54% 52%
Dendritic cell, mouse, mature, BALB/c NF 49% 78%
Dendritic cell, mouse, mature, C57BL/6 NF 37% 63%
Dendritic cell, mouse, immature, BALB/c 96 27 – 43% 37 – 49%
Dendritic cell, mouse, immature, C57BL/6 96 23 – 34% 41 – 58%
Dendritic cell, mouse, mature, BALB/c 96 32% 85%
Dendritic cell, mouse, mature, C57BL/6 96 29% 88%
Macrophage, human NF 55 – 59% 87 – 88%
Macrophage, human 96 42% 60%
Macrophage, mouse, BALB/c NF 34 – 45% 84 – 92%
Macrophage, mouse, C57BL/6 NF 24 – 47% 80 – 88%
Monocyte, human NF 55 – 56% 62 – 81%
Monocyte, human 96 64% 77%
Natural killer (NK) cell, human NF 16% 50 – 60%
T cell, human, stimulated NF 36 – 47%
T cell, human, unstimulated NF 66%
T cell, human, stimulated 96 70% 59%
T cell, human, unstimulated 96 68 – 80% 53 – 79%
T cell, mouse, BALB/c NF 32 – 44% 18 – 55%
T cell, mouse, C57BL/6 NF 20 – 28% 17 – 45%
T cell, mouse, BALB/c 96 45% 32%
T cell, mouse, C57BL/6 96 43% 23%
Dermal Cells
Fibroblast, dermal (NHDF), human, adult NF 42 – 69% 74 – 77%
Fibroblast, dermal (NHDF), human, neonatal NF 82 – 90%
Fibroblast, dermal (NHDF), human, neonatal 96 86 – 98% 86 – 91%
Keratinocyte, human, adult NF 36 – 51% 40 – 60%
Keratinocyte, human, neonatal NF 39 – 53% 50 – 60%
Melanocyte, (NHEM-neo), human, neonatal NF 70% 55 – 60%
Endothelial Cells
Endothelial cell, human, coronary artery (HCAEC) NF 57% 42%
Endothelial cell, human, lung (HMVEC-L) NF 52% 52%
Endothelial cell, human, umbilical vein (HUVEC) NF 90% 74%
Endothelial cell, human, umbilical vein (HUVEC) 96 74% 66%
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List of Nucleofector® Kits for Primary Cells
Continued
Nucleofector® Kits
Cell Type Kit Transfection Viability
Efficiency
Epithelial Cells
Epithelial cell, human, bronchial (NHBE) NF 52% 51%
Epithelial cell, human, bronchial (NHBE) 96 54% 53%
Epithelial cell, human, mammary (HMEC) NF 56 – 73% 66 – 98%
Epithelial cell, human, mammary (HMEC) 96 51% 66%
Epithelial cell, human, prostate (PrEC) NF 43% 64%
Epithelial cell, human, prostate (PrEC) 96 67% 48%
Hepatocytes
Hepatocyte, human 96 54% 59 – 69%
Hepatocyte, mouse NF 54% 80%
Hepatocyte, rat NF 52% 78%
Neural Cells
Astrocyte, mouse NF 60% 60 – 70%
Astrocyte, rat NF 59-67% 70 – 80%
Neuron, chicken, hippocampal NF 43%
Neuron, chicken, dorsal root ganglion NF 30%
Neuron, mouse, hippocampal NF 58%
Neuron, mouse, dorsal root ganglion NF 30 – 60% 25 – 65%
Neuron, rat, hippocampal/cortical NF 54 – 64% 47 – 52%
Neuron, rat, hippocampal/cortical 96 60%
Neuron, rat, dorsal root ganglion NF 41%
Oligodendrocyte, rat NF 44% 60%
Stem Cells
CD34+ cell, human NF 71%
Embryonic stem cell (H9), human 96 64% 98%
Embryonic stem cell, human NF 20 – 78% 50 – 96%
Embryonic stem cell, mouse NF 80%
Embryonic stem cell, mouse 96 77 – 90% 68 – 81%
Mesenchymal stem cell (MSC), human NF 30 – 47% 78 – 86%
Mesenchymal stem cell (MSC), human 96 67%
NOTE: Nucleofector® Kits contain a
Neural stem cell (NSC), mouse NF 40 – 60% proprietary nucleic acid coding for
a proprietary copepod fluorescent
Neural stem cell (NSC), rat NF 42 – 46%
protein intended to be used as positive
control with this Lonza product only.
Any use of proprietary nucleic acid
Other Cells or fluorescent protein other than
Cardiomyocyte, rat, neonatal NF 35% 50 – 60% as positive control with this Lonza
product is strictly prohibited. USE IN
Chondrocyte, human NF 65% 60 – 70% ANY OTHER APPLICATION REQUIRES
Chondrocyte, human 96 74% 84% LICENSE FROM EVROGEN. To obtain
such a license, please contact Evrogen
Embryonic fibroblast (MEF), mouse NF 43% 60 – 80% at license@evrogen.com.
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Human B Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
% CAT activity
gene, a setup required in rescue experiments or as 350
transfection control. 300
Promoter studies in primary B cells prove that NF-κB regulates the activity
of the human CR2 promoter. Primary human B cells were transiently
co-transfected with a CAT reporter plasmid driven by wild-type (WT) CR2
promoter, and plasmids encoding NF-κB subunit p50 or p65 or a control
plasmid. Cells were assayed for CAT activity 15 hours post Nucleofection®.
Values represent percentage of CAT activity, considering the activity of the
empty vector control 0% and the activity of the wild-type CR2 promoter 100%.
The results demonstrate that both NF-κB subunits clearly induce CAT activity.
(Data reproduced from Tolnay et al. (2002) J Immunology, with permission
from the Journal of Immunology.)
Buffy 2
60
– Suitable for siRNA, shRNA, DNA plasmids, etc. 50
40
Technical Information
30
20
10
Clonetics®
C // C C
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 66 68 70 72 74 76 78 80 82 84 86 88 90 92 94 96
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Mouse B Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Mouse B cells are a frequently used model system in Ordering Information
various research fields, such as tumor immunology,
Cat. No. Size Price
signal transduction and B cell development. However,
transfection efficiencies reached by non-viral methods Mouse B Cell Nucleofector® Kit
like lipofection or electroporation were far below scientists'
VPA-1010 25 reactions
expectations. The new Mouse B Cell Nucleofector® Kit
will bridge this methodological gap, therefore enabling
scientific approaches that are otherwise impossible with
today’s technology.
– Up to 59% efficiency and 47% viability
– Expression of cell typical marker proteins not effected A B
– One protocol for DNA, siRNA and RNA transfection
Mouse B cells play a central role in the humoral immune Ordering Information
response, thus understanding their functionality for
Cat. No. Size Price
example by loss and gain of function studies is of vital
interest for research groups in academia and industry. Mouse B Cell 96-well Nucleofector® Kit
The Mouse B Cell 96-well Nucleofector® Kit for stimulated
VHPA-1010 96 reactions
B cells allows high throughput transfection with a wide
VHPA-2010 960 reactions
variety of substrates enabling scientists to perform these
studies with much less time and labor input.
– Allowing best performance on various donor strains
– Up to 55% transfection efficiency
– Cells maintain their functionality A B
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Human CD34+ Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
% Positive cells
% Positive cells
– Up to 70% transfection efficiency 40 40
– No influence on hematopoietic cell differentiation 30 30
– Cited for DNA and siRNA transfection 20 20
% Viability
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Mouse Dendritic Cell Nucleofector® Kit
Nucleofector® Kits
Neurons
Lonza’s R&D team has developed a Nucleofector® Kit for Ordering Information
the transfection of immature and mature mouse dendritic
Cat. No. Size Price
cells. For the first time, it is possible to investigate the
role and function of dendritic cells in the murine model Mouse Dendritic Cell Nucleofector® Kit
system using a non-viral transfection approach. Gene
VPA-1011 (Immature) 25 reactions
over-expression studies or RNAi mediated gene silencing
VPA-1012 (Mature) 25 reactions
can then easily be performed in these cells that play such
a pivotal role within immune defense.
A B
110 Control 110 Efficiency
% Functionality
% Transfection efficiency
Sample Viability
100 100
Functionality post Nucleofection®. The graph displays functionality of 90 90
immature mouse DCs (isolated from mice strain Balb/C) post Nucleofection® 80 80
(Sample) on the standard Nucleofector® Device. 2 hours post Nucleofection®, 70 70
cells were stimulated by LPS. 22 hours later, functionality was analyzed by
IL-6 specific ELISA and is given in percent compared to non-transfected 60 60
control. (A) 50 50
40 40
Transfection Performance. Mouse DC (Balb/C) were transfected according
30 30
to the appropriate optimized protocol for Nucleofection® using pmaxGFP®
Vector. Cells were analyzed 24 hours post Nucleofection® by flow cytometry 20 20
for maxGFP® Reporter Protein expression and viability. Cell viability is given 10 10
in percent compared to non-transfected control. (B) 0 0
Mouse dendritic cells play a central role in the mammalian Ordering Information
immune system. Understanding dendritic cell functionali- Cat. No. Size Price
ty is of vital interest for both research groups and industry.
The two Mouse Dendritic Cell 96-well Nucleofector® Kits Mouse Dendritic Cell 96-well Nucleofector® Kits
allow high throughput transfection with a wide variety of
VHPA-1011 (Immature) 96 reactions
substrates, enabling scientists to perform such studies
VHPA-1012 (Mature) 96 reactions
more efficiently than ever before. Reproducible high
VHPA-2011 (Immature) 960 reactions
performance is guaranteed for both mature and immature
VHPA-2012 (Mature) 960 reactions
mouse dendritic cells using kits dedicated for either
mature or immature cells.
– Up to 43% transfection efficiency
– High cell viability (up to 80%) 100
% Transfection efficiency
60
50
Well-to-well uniformity of reporter gene expression of mouse dendritic
40
cells transfected by 96-well Nucleofection®. Mouse dendritic cells were
transfected by Nucleofection® with the pmaxGFP® Vector using the Mouse 30
Dendritic Cell 96-well Nucleofector® Kit. 24 hours post Nucleofection®, 20
cells were analyzed on a BD FACSCalibur™ Flow Cytometer with HTS option. 10 C C C C // C C C C
Wells without GFP expression are negative controls of cells in Nucleofector®
Solution and plasmid DNA, but without Nucleofection®. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
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Human Macrophage Nucleofector® Kit
Nucleofector® Kits
Blood Cells
C D
24 hours post Nucleofection®, SEAP enzyme activity was measured (n=36, 0.2
SD = ± 14% from mean). Wells without SEAP enzyme activity are negative 0.1 C C // C
controls of cells in 96-well Nucleofector® Solution and plasmid DNA but 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
without Nucleofection®. Well C == Control
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Mouse Macrophage Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Macrophages are frequently used in studies on basic Ordering Information
immunology and cancer research. With the Mouse
Cat. No. Size Price
Macrophage Nucleofector® Kit, you can now address
areas such as gene regulation, signaling pathways or Mouse Macrophage Nucleofector® Kit
identification of genes responsible for differentiation in
VPA-1009 25 reactions
the murine in vitro model system.
– Evaluated for macrophages from C57BL/6 and
BALB/c strains
– For transfection of resting bone-marrow derived
macrophages
– Maintenance of functionality (e.g., activation)
A B
C D
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Human Monocyte Nucleofector® Kits
Nucleofector® Kits
Blood Cells
A B
200 12
96.37%
10
160
8
120
6
80
4
40 2
0 10 101 102 103 104 0 – Control PPARg #1
FL1-H sRNAi
C D
% CX3CR1+ cells
45
40 20
35
30 15
25
20 10
15
10 5
5
0 – 9-HODE 0 – 9-HODE
Lipid Lipid
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Human Monocyte 96-well Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Human monocytes are involved in the pathogenesis Ordering Information
of atherosclerosis. They play an important role in Cat. No. Size Price
immune defense, inflammation and tissue remodeling.
Understanding these processesis a key issue for drug Human Monocyte 96-well Nucleofector® Kits
development. The Human Monocyte 96-well Nucleofector®
VHPA-1007 96 reactions
Kit is tailored to high throughput application needs in
VHPA-2007 960 reactions
industry and academia.
– First high throughput transfection technology for Human Monocyte Nucleofector® Medium
human monocytes
VZB-1003 1 x 500 ml
– Excellent well-to-well reproducibility
VZB-4003 4 x 500ml
– 75% cell viability
– Up to 76% transfection efficiency
– 80% fewer cells required compared to standard
Nucleofector® Device
100
% Transfection efficiency
90
80
70
60
50
40
30
20
10 C C // C C C C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C==Control
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Human Natural Killer Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
SSC
0.5% 16.9%
eGFP eGFP
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Human T Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
The Nucleofection® of unstimulated and stimulated human Ordering Information
T cells has become a significant tool in immunology
Cat. No. Size Price
research. For optimal transfection results, Lonza offers
specially developed optimized protocols for stimulated Human T Cell Nucleofector® Kit
and unstimulated T cells. A huge number of publications
VPA-1002 25 reactions
in which T cells have been transfected with DNA plasmids,
shRNA vectors and siRNA prove that T cells transfected
by Nucleofection® maintain functionality. RNAi-mediated A B C
gene silencing could thus be shown to address a variety
of scientific questions in primary T cells.
side scatter
– For unstimulated and stimulated T cells
CD3-APC
– Up to 70% transfection efficiency
PJ
forward scatter FL1 FL2
D + DNA E + DNA
0.14% 60.64%
CD3-APC
stained with antibody directed against CD3. Dead cells were excluded by
propidium iodide staining and gating (B/C). maxGFP® Reporter Protein
expression of T cells is shown after Nucleofection® without (D) and with
plasmid DNA (E). FL1 FL1
Buffy 2
– Transfected cells preserve their biochemical 90
functionality 80
70
60
50
Well-to-well uniformity of reporter gene expression of human T cells
40
transfected with the Nucleofector® 96-well Shuttle® System. Human T
cells were transfected by Nucleofection® with pmaxGFP® Vector using the 30
Human T Cell 96-well Nucleofector® Kit. 24 hours post Nucleofection®, cells 20
were analyzed on a BD FACSCalibur™ Flow Cytometer with HTS option. Wells 10 C C C C
without GFP expression are negative controls of cells in Nucleofector® //
149
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Mouse T Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
150
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Human Dermal Fibroblast (NHDF) Nucleofector® Kit
Nucleofector® Kits
Dermal Cells
Human dermal fibroblasts are used to study the Ordering Information
biochemical basis of various normal and disease
Cat. No. Size Price
processes. The efficient and reproducible transfection
of NHDFs with the Nucleofector® Technology has Human Dermal Fibroblast (NHDF) Nucleofector® Kit
provided a major advancement in studying diseases
VPD-1001 25 reactions
related to fibroblast cells such as fibrosarcoma, fibrosis,
scleroderma, and xeroderma pigmentosum.
– Up to 90% transfection efficiency
A B
Related Products
Nucleofection® of adult human dermal fibroblasts with eGFP cDNA. Adult
NHDF were transfected by Nucleofection® using the Human Dermal NHDF-Ad - Human Adult Dermal Fibroblasts 38
Fibroblast Nucleofector® Kit, a plasmid encoding eGFP and program
U-23/U-023. 24 hours post Nucleofection®, cells were analyzed by light (A) NHDF-Neo - Neonatal Human Dermal Fibroblasts 38
and fluorescence microscopy (B). FGM®-2 BulletKit® 38
0.1 µg maxGFP™
0.4 µg maxGFP™
Human
100
Dermal Fibroblast (NHDF) 96-well Nucleofector® Kit
% Transfection efficiency
90
80
70
60
50
40
Human dermal fibroblasts are commonly used to
30 Ordering Information
20
elucidate the molecular10
basis of various skin diseases.
C C // C Cat. No. Size Price
Pharmacological
2 4 6 8 10 12 14studies
16 18 20 22 24 to
0 26 28reveal
30 32 66 68the
70 72 74function of88 genes
76 78 80 82 84 86 90 92 94 96
Well C = Control
related to fibroblast based diseases, like fibrosarcoma NHDF 96-well Nucleofector® Kits
or fibrosis, involve high throughput knockdown using
VHPD-1001 96 reactions
siRNA. With the new Human Dermal Fibroblast 96-well
VHPD-2001 960 reactions
Nucleofector® Kit, it is possible to perform Nucleofection®
of various substrates in 96-well format.
0.1 µg pmaxGFP® Vector 0.4 µg pmaxGFP® Vector
100
% Transfection efficiency
90
80
70
60
50
40
30
20
10 C C // C
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 66 68 70 72 74 76 78 80 82 84 86 88 90 92 94 96
Well C = Control
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Human Keratinocyte Nucleofector® Kit
Nucleofector® Kits
Dermal Cells
152
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NHEM-Neo (Normal Human Epidermal Melanocyte-Neo) Nucleofector® Kit
Nucleofector® Kits
Dermal Cells
Melanocytes, the pigment-producing cells of the epider- Ordering Information
mis, undergo malignant transformation in malignant
Cat. No. Size Price
melanoma. Melanoma cells can metastasize to almost
every major organ and tissue. Nucleofection® of neonatal NHEM-Neo Nucleofector® Kit
melanocytes is a major pre-requisite to elucidate this
VPD-1003 25 reactions
transformation process.
– Up to 80% transfection efficiency
– Reproducible non-viral transfection
– For DNA and siRNA transfection
A B
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Basic Nucleofector® Kit for Primary Mammalian Fibroblasts
Nucleofector® Kits
Dermal Cells
cadherin-11
tubulin 2+3
relative intensity 1 0.89 0.99
SIN-CAD
3+4
Related Products
Fibroblast Cells 36 – 39
FGM®-2 BulletKit® 38
154
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Basic 96-well Nucleofector® Kits for Primary Mammalian Fibroblasts
Nucleofector® Kits
Dermal Cells
Fibroblasts are commonly used to elucidate the molecular Ordering Information
basis of various fibroblast-based diseases like fibrosarco-
Cat. No. Size Price
ma or fibrosis. Furthermore, fibroblasts are utilized as
"feeder cells" in human embryonic stem cell research Basic 96-well Nucleofector® Starter Kit for
and for reprogramming into iPS (induced pluripotent Primary Mammalian Fibroblasts
stem) cells. The Basic 96-well Nucleofector® Starter
VHPI-1002 64 reactions
Kit and the Basic 96-well Nucleofector® Kits 1 and 2 for
Primary Mammalian Fibroblasts allow high throughput Basic 96-well Nucleofector® Kit 1 for
Nucleofection® with any type of mammalian fibroblasts. Primary Mammalian Fibroblasts
– Economical starter kit for fast determination
VHPI-1012 96 reactions
of ideal Nucleofection® conditions
VHPI-2012 960 reactions
– Two specialized basic kits suited to your
specific cell type Basic 96-well Nucleofector® Kit 2 for
Primary Mammalian Fibroblasts
VHPI-1022 96 reactions
A B
VHPI-2022 960 reactions
Related Products
Fibroblast Cells 36 – 39
FGM®-2 BulletKit® 38
155
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Human Coronary Artery Endothelial Cell (HCAEC) Nucleofector® Kit
Nucleofector® Kits
Endothelial Cells
A B
A B
Example for the Nucleofection® of HMVEC-L. HMVEC-L were transfected by Related Products
Nucleofection® using the HMVEC-L Nucleofector® Kit and a plasmid encoding
the enhanced green fluorescent protein eGFP. 25 hours post Nucleofection®, HMVEC-L - Lung Microvascular Endothelial Cells 26
cells were analyzed by light (A) and fluorescence microscopy (B). EGM®-2MV BulletKit® 33
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Human Umbilical Vein Endothelial Cell (HUVEC) Nucleofector® Kit
Nucleofector® Kits
Endothelial Cells
HUVEC is an easy-to-isolate endothelial cell type, Ordering Information
commonly used in cancer and cardiovascular research to
Cat. No. Size Price
address areas such as angiogenesis and cardiovascular
diseases. Using the HUVEC Nucleofector® Kit, high HUVEC Nucleofector® Kit
transfection efficiencies and protein expression levels can
VPB-1002 25 reactions
be achieved. It is the ideal tool for protein over-expression
studies or RNAi experiments.
3.5
Chemiluminescence (x104 cpm)
3.0 SOD
- siRNA
2.0
NADH
1.0 + siRNA
0
10 20 30
Time (min)
The 96-well Nucleofector® Kit for HUVEC cells allows non Ordering Information
viral, high throughput transfection of HUVEC with up to 96
Cat. No. Size Price
different substrates in parallel. It opens the possibility to
perform projects like siRNA screening within your drug HUVEC 96-well Nucleofector® Kits
discovery process.
– Excellent performance with up to 85% efficiency VHPB-1002 96 reactions
80
% Transfection efficiency
70
60
50
40
30
20
10 C C C C // C C C C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C == Control
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Basic Nucleofector® Kit for Primary Mammalian Endothelial Cells
Nucleofector® Kits
Endothelial Cells
The Basic Nucleofector® Kit for Primary Endothelial Cells Ordering Information
is the ideal tool to transfect a multitude of endothelial
Cat. No. Size Price
cells from different mammalian species (e.g., mouse, rat,
human) and from various organs (e.g., lung, brain, liver). Basic Nucleofector® Kit for
– Suitable for virtually any primary mammalian Primary Mammalian Endothelial Cells
endothelial cell type
VPI-1001 25 reactions
– Testing of just five selected programs with one
Nucleofector® Solution
– Already tested for porcine capillary endothelial cells,
sheep uterine artery endothelial cells, etc.
A B
Related Products
Endothelial Cells & Media 23 – 35
EGM®-2 BulletKit® 35
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Normal Human Bronchial Epithelial Cell (NHBE) Nucleofector® Kit
Nucleofector® Kits
Epithelial Cells
Revealing a gene’s role in human bronchial cells may help Ordering Information
in understanding the formation and progression of human
Cat. No. Size Price
lung carcinoma. Transfection of primary human bronchial
epithelial cells isolated from a carcinoma is an important Normal Human Bronchial Epithelial Cell (NHBE)
method to study gene function. The NHBE Nucleofector® Nucleofector® Kit
Kit enables you to easily verify previous cell line results in
VPK-1001 25 reactions
the analogous primary cell type.
A B
Related Products
Example of Nucleofection® of NHBE. Normal human bronchial epithelial
cells were transfected with 2 µg pmaxGFP® Vector using the Normal Human
Bronchial Epithelial Cell Nucleofector® Kit. 24 hours post Nucleofection®, NHBE - Bronchial /Tracheal Epithelial Cells 19
cells were analyzed by light (A) or fluorescence (B) microscopy. BEGM® BulletKit® 19
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
C C C C C C C C
0.1
//
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C = Control
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Human Mammary Epithelial Cell (HMEC) Nucleofector® Kit
Nucleofector® Kits
Epithelial Cells
Lucif.-SiRNA
Rit42-siRNA
Related Products
HMEC - Mammary Epithelial Cells 48
MEGM® BulletKit® 48
160
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Human Mammary Epithelial Cell (HMEC) 96-well Nucleofector® Kit
Nucleofector® Kits
Epithelial Cells
HMECs are the ideal model system to elucidate the role of Ordering Information
breast cancer related genes in a primary tissue context.
Cat. No. Size Price
However, time and labor efficient high throughput
studies were hard to perform in these cells due to Human Mammary Epithelial Cell (HMEC)
the lack of appropriate and easy-to-use transfection 96-well Nucleofector® Kits
systems. The Human Mammary Epithelial Cell (HMEC)
VHPK-1002 96 reactions
96-well Nucleofector® Kit now renders RNAi mediated
VHPK-2002 960 reactions
gene silencing, as well as over-expression approaches
possible.
– Up to 51% efficiency
– Around 66% viability
– Low well-to-well variation
– No lot-to-lot variations
1.4
A405
1.3
1.2
1.1
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
C C CC C C C C
0.1
//
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C == Control
Related Products
HMEC - Mammary Epithelial Cells 48
MEGM® BulletKit® 48
161
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Human Prostate Epithelial Cell (hPrEC) Nucleofector® Kit
Nucleofector® Kits
Epithelial Cells
A B
Related Products
Example for Nucleofection® of human PrECs. Normal human prostate epithelial
cells were transfected with 2 µg pmaxGFP® Vector using the Human Prostate PrEC - Prostate Epithelial Cells 52
Epithelial Cell (hPrEC) Nucleofector® Kit. 24 hours post Nucleofection®, cells
were analyzed by light (A) or fluorescence microscopy (B). PrEGM™ BulletKit® 52
Normal human prostate epithelial cells are often used Ordering Information
to study potential causes of cancer by knock down or
Cat. No. Size Price
induction of cancer related genes. The new hPrEC 96-well
Nucleofector® Kit is the perfect tool for such projects. Human Prostate Epithelial Cell (hPrEC)
96-well Nucleofector® Kits
VHPK-1003 96 reactions
1.0 VHPK-2003 960 reactions
A405
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1 C C C C
//
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C == Control
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Basic Nucleofector® Kit for Primary Mammalian Epithelial Cells
Nucleofector® Kits
Epithelial Cells
The Basic Nucleofector® Kit for Primary Epithelial Cells is Ordering Information
the ideal tool to transfect a multitude of epithelial cells from
Cat. No. Size Price
different mammalian species (e.g., mouse, rat, human)
and from various organs (e.g., lung, liver, kidney). Basic Nucleofector® Kit for
Primary Mammalian Epithelial Cells
VPI-1005 25 reactions
Example for Nucleofection® of primary human renal proximal tubular epithelial cells. Human renal proximal tubular epithelial
cells were transfected by Nucleofection® with the Basic Nucleofector® Kit for Primary Mammalian Epithelial Cells, program
U-17/U-017 and a plasmid encoding the green fluorescent protein, eGFP. 48 hours post Nucleofection®, cells were analyzed
by fluorescence microscopy.
(Data courtesy of Chang Xu, Robert L. Bacallao*, and Seth L. Alper. Department of Medicine, Beth Israel Deaconess Medical
Center and Harvard Medical School, Boston, Massachusetts, USA, *University of Indiana School of Medicine, Indianapolis,
USA.)
With the new Basic 96-well Nucleofector® Kit for Primary Ordering Information
Mammalian Epithelial Cells, Nucleofection® of epithelial
Cat. No. Size Price
cells, is now a high throughput application. It can be used
for screening approaches with DNA as well as siRNA and Basic 96-well Nucleofector® Kits for
shRNA vectors. Primary Mammalian Epithelial Cells
VHPI-1005 64 reactions
The Approach
Related Products
Airway Epithelial Cells & Media 18 – 20
Renal Cells & Media 54 – 57
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Human Hepatocyte 96-well Nucleofector® Kit
Nucleofector® Kits
Hepatocytes
A B
0.25
Albumin secretion [pg/cell/hour]
0.2
0.15
0.1
0.05
Nucleofection® of mouse hepatocytes. Primary mouse hepatocytes were Hepatocytes transfected by Nucleofection® maintain functionality. 24 hours
transfected with 4 µg pmaxGFP® Vector using the Mouse Hepatocyte post-plating, primary mouse hepatocytes transfected by Nucleofection®
Nucleofector® Kit. 24 hours post Nucleofection®, cells were analyzed by and non Nucleofection® Transfected Control Cultures were analyzed for
light (A) and fluorescence microscopy (B). albumin secretion (normalized to cell number) by ELISA.
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Rat Hepatocyte Nucleofector® Kit
Nucleofector® Kits
Hepatocytes
Rat hepatocytes are involved in numerous metabolic Ordering Information
pathways and have important functions that include
Cat. No. Size Price
detoxification and synthesis of proteins, lipids and
bile acids. With the first efficient non-viral transfection Rat Hepatocyte Nucleofector® Kit
method for primary rat hepatocytes in hand, you can now
VPL-1003 25 reactions
get a better understanding of new therapeutic agents and
toxicity mechanisms
– Up to 55% transfection efficiency
– Up to 80% viability
– Suitable for both DNA and siRNA
– Maintenance of functional properties
A B
N
*
** *
N
N N
N
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Mouse Astrocyte Nucleofector® Kit
Nucleofector® Kits
Neural Cells
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Chicken Neuron Nucleofector® Kit
Nucleofector® Kits
Neural Cells
Chicken neurons are used as a model system to elucidate Ordering Information
neurological disorders, such as Parkinson’s Disease. The
Cat. No. Size Price
Nucleofector® Technology is providing a new insight that
will enable you to learn more about neurodegenerative Chicken Neuron Nucleofector® Kit
diseases using this in vitro model system.
– Transfection efficiencies of more than 40% VPG-1002 25 reactions
C
Formation of normal growth cones indicates maintenance of functionality
of dorsal root ganglia after Nucleofection®. DRG neurons from chicken were
transfected by Nucleofection® with a plasmid encoding the GFP protein.
After cultivation on pre-coated glass coverslips overnight, single cells were
analyzed for formation of normal growth cones (A-C), F-actin localization
after staining with Alexa 568 conjugated phalloidin (A and C) and GFP
expression (B and C).
(Photograph courtesy of B. Eickholt, King’s College, London, Great Britain.)
A B
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Rat Neuron Nucleofector® Kit
Nucleofector® Kits
Neural Cells
properties
– Proven performance for siRNA, shRNA, miRNA, and
antisense oligos
Rat neurons are a versatile in vitro model system to study Ordering Information
neural cell function and interaction of neurons in the
Cat. No. Size Price
nervous system. Efficient high throughput transfection
of these cells is a key issue to elucidate neurolo- Rat Neuron 96-well Nucleofector® Kits
gical pathways. With the Rat Neuron 96-well Nucleofector®
Kit, this gap is closed. High throughput Nucleofection® of VHPG-1003 96 reactions
A B
➔
96-well Nucleofection® of neonatal rat hippocampal neurons. Freshly Normal neuronal development after 96-well Nucleofection®. Freshly isolated
isolated rat cells (P0) were transfected by Nucleofection® with program neonatal rat hippocampal neurons (P0) transfected by Nucleofection® with
96-CU-133 and pSyn-GFP (eGFP under control of the synapsin promoter). pSyn-GFP and pDsRed were plated onto glass coverslips and examined
After 7 DIV, neurons were fixed and analyzed by light (A) and fluorescence after 7 DIV for GFP (green) and RFP (red) expression. Transfected neurons
(B) microscopy. Neuron morphology was unaltered compared to non- show an extensive dendritic network and develop dendritic protrusions that
transfected neurons. already resemble mature mushroom shaped dendritic spines (arrows).
Surrounding, transfected glia cells are shown by DAPI staining (blue).
(Data courtesy of M. Kiebler, Department of Neuronal Cell Biology, Medical
University of Vienna, Vienna, Austria.)
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Rat Oligodendrocyte Nucleofector® Kit
Nucleofector® Kits
Neural Cells
During multiple sclerosis, oligodendrocytes as well as Ordering Information
the myelin sheath which insulates axons, are destroyed.
Cat. No. Size Price
Nucleofector® Technology is the first technology which
permits an efficient non-viral gene transfer into these Rat Oligodendrocyte Nucleofector® Kit
cells. This provides further insights into oligodendrocyte
biology and dysfunction. VPG-1009 25 reactions
100
% Transfection efficiency
DsRed
EGFP
90
80
Comparison of conventional electroporation, lipofection and Nucleofection® 70
for transfection of rat neuronal progenitor cells. Ventral mesencephalic
progenitor (VMP) cells from rat brain, which are the important source 60
of dopaminergic neurons for cell replacement strategies in Parkinson’s 50
Disease, were transfected with two different plasmids expressing DsRed 40
or EGFP. For transfection, conventional electroporation (EasyjecT from 30
EquiBio, 100 µg plasmid per 500,000 cells), lipofection (Lipofectamine™
20
2000 Reagent, 0.5 µg DNA per 60,000 cells), or Nucleofection® (5 µg DNA
per 2,000,000 cells) were used. 10
(Data from Cesnulevicius et al. (2006) Stem Cells 24(12), 2776-91.) 0 Electroporation Lipofection Nucleofection®
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Basic 96-well Nucleofector® Kit for Primary Mammalian Neurons
Nucleofector® Kits
Neural Cells
the unique Nucleocuvette® Plate guarantees the most VHPI-2003 960 reactions
The Approach
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Basic Neuron SCN Nucleofector® Kit
Nucleofector® Kits
Neural Cells
The Basic Neuron Small Cell Number (SCN) Nucleofector® Ordering Information
Kit makes high efficiency transfection of mammalian
Cat. No. Size Price
primary neurons possible for extremely low cell numbers.
Now you can transfect just the number of cells needed Basic Neuron SCN Nucleofector® Kit
for later culture and analysis (e.g. microscopic analysis
VSPI-1003 25 reactions
of neuron growth and morphology). Benefit from a drastic
reduction in both number of donor animals and amount
of preparation time. The new, optimized SCN Nucleofector®
Solution, SCN Cuvettes and Programs make transfection
efficiencies of up to 60% possible with down to 15,000
cells per Nucleofection®.
– Transfection of down to 15,000 cells per Nucleofection®
(i.e., more experiments with fewer donor animals)
– Excellent non-viral transfection efficiencies
and viabilities (i.e., 50% and higher even for very
difficult-to-transfect neurons like DRGs)
A B
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Human Aortic Smooth Muscle Cell Nucleofector® Kit
Smooth Muscle Cells
Nucleofector® Kits
Example for the transfection of human AoSMC with eGFP. Human AoSMC
were transfected by Nucleofection® using the Human AoSMC Nucleofector®
Kit, program U-25/U-025 and a plasmid encoding the enhanced green
fluorescent protein eGFP. 14 hours post Nucleofection®, the cells were
analyzed by light (A) or, fluorescence microscopy (B).
90
80
70
60
50
40
30
20
10
0 1d 2d 7d 14 d 21 d 28 d
Related Products
AoSMC - Aortic Smooth Muscle Cells 65
SmGM®-2 BulletKit® 65
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Basic Nucleofector® Kit for Primary Smooth Muscle Cells
Smooth muscle cells (SMCs) can be found within the tunica Ordering Information
media layer of arteries and veins, the bladder, uterus,
Cat. No. Size Price
male and female reproductive tracts, gastrointestinal
tract, respiratory tract, the ciliary muscle, and iris of Basic 96-well Nucleofector® Kits for
the eye. Vascular smooth muscle cells represent a Primary Smooth Muscle Cells
well established system to study vascular disorders
such as atherosclerosis and stroke. The Basic 96-well VHPI-1004 96 reactions
Nucleofector® Kit for Primary Smooth Muscle Cells allows VHPI-2004 960 reactions
high throughput Nucleofection® with almost any type of
mammalian SMCs.
A B
Nucleofection® of primary smooth muscle cells. Primary pulmonary artery Related Products
smooth muscle cells were transfected with 0.2 µg the pmaxGFP® Vector
using the Basic 96-well Nucleofector® Kit for Primary Smooth Muscle
Cells. 24 hours post Nucleofection®, cells were analyzed by light (A) and Smooth Muscle Cells & Media 64 – 68
fluorescence (B) microscopy. SmGM®-2 BulletKit® 65
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Human Stem Cell Nucleofector® Kit
Nucleofector® Kits
Stem Cells
Embryonic stem cells (ES cells) are derived e.g. from the Ordering Information
inner cell mass of preimplantation embryos and retain the
Cat. No. Size Price
developmental potency of embryonic founder cells, being
able to differentiate into cells and tissues of all three germ Human Stem Cell Nucleofector® Starter Kit
layers in vitro and in vivo. Since ESCs can be differentiated
into many cell types in vitro, they provide a resource not VPH-5002 18 reactions
only for studying basic human developmental biology, Human Stem Cell Nucleofector® Kit 1
but also for therapeutic clinical approaches to replace
VPH-5012 25 reactions
diseased cells in humans.
– Tedious creation of viruses unnecessary Human Stem Cell Nucleofector® Kit 2
– Higher transfection efficiencies compared
VPH-5022 25 reactions
to other methods
– Less DNA and lower cell number needed
100 Efficiency
Transfection efficiency / viability (%)
Viability
90
80
70
60
50
40
30
20
10
0 H1 H9 H 9.2 Hues-9 BG01V Customer
Related Products
NHNP - Normal Human Neural Progenitors Cells 97
174
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Basic 96-well Nucleofector® Kits for Human Stem Cells
Nucleofector® Kits
Stem Cells
Human embryonic stem cells are the subject of Ordering Information
increasing scientific interest because of their potential
Cat. No. Size Price
utility in numerous biomedical applications. Since stem
cells are a self-renewing population of cells, they can Basic 96-well Nucleofector® Starter Kit for
be continuously cultured in an undifferentiated state Human Stem Cells
and give rise to more specialized cells of the body, such
VHPH-5002 64 reactions
as heart, liver, fat, blood and nerve cells. The new Basic
96-well Nucleofector® Kit for Human Stem Cells now Basic 96-well Nucleofector® Kit 1 for Human Stem Cells
allows high throughput Nucleofection® of other human
VHPH-5012 96 reactions
embryonal stem cell lines combined with the ability to use
VHPH-5212 960 reactions
low cell numbers.
– Economical starter kit for fast determination of ideal Basic 96-well Nucleofector® Kit 2 for Human Stem Cells
Nucleofection® conditions VHPH-5022 96 reactions
– Successfully tested for H1, H9, H14 and HS306 cells VHPH-5222 960 reactions
A B
Related Products
hMSC - Human Mesenchymal Stem Cells 90
NHNP - Nomal Human Neural Progenitors Cells 97
175
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Human Stem Cell (H9) 96-well Nucleofector® Kit
Nucleofector® Kits
Stem Cells
Nucleofection® of the stem cell line H9 in a 96-well format, VHPH-5203 960 reactions
thus enabling high throughput approaches to elucidate
various aspects of stem cell differentiation.
– 64% transfection efficiency
– Up to 98% cell viability
– Excellent preservation of pluripotency
% Positive cells
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Mouse ES Cell Nucleofector® Kit
Nucleofector® Kits
Stem Cells
Mouse ES cells offer the ability to differentiate into various Ordering Information
cell types and tissues, enabling them to be employed for
Cat. No. Size Price
cell differentiation studies or the generation of knock-out
mice. Mouse ES Cell Nucleofector® Kit
– Tested with several mouse ES cell lines
VPH-1001 25 reactions
(e.g., R1, D3, E14)
– Successfully used to generate germline chimeras
– Homogenous transient gene expression pattern
– No influence on in vitro or in vivo A-13 no DNA EP
differentiation potential
Mouse ES cells are often used to study the biological Ordering Information
basics of cell differentiation, tissue formation and embryo
Cat. No. Size Price
development. In addition, transfected Mouse ES cells are
used to generate knock-out mice. Mouse ES Cell 96-well Nucleofector® Kit
– Transfection efficiencies up to 90%
– Viability of up to 80% after 24 hours VHPH-1001 96 reactions
(ability to differentiate)
100 Transfection efficiency
Transfection efficiency / viability (%)
Viability
90
80
70
60
50
40
30
20
10
0 E14 D3
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Human MSC (Mesenchymal Stem Cell) Nucleofector® Kit
Nucleofector® Kits
Stem Cells
100 Efficiency
% Transfection efficiency
Viability
90
80
70
60
50
40
30
20
10
0 Nucleofection® FUGENE® 6 DOTAP
Related Products
hMSC - Human Mesenchymal Stem Cells 90
MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit® 90
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Mouse NSC (Neural Stem Cell) Nucleofector® Kit
Nucleofector® Kits
Stem Cells
NSC from mouse brain can be differentiated into various Ordering Information
neural and glial cell types. This may potentially contribute
Cat. No. Size Price
to the development of therapies for CNS disorders via
replacement of damaged neural tissue. The Nucleofector® Mouse NSC (Neural Stem Cell) Nucleofector® Kit
Technology presents a powerful tool to gain further
VPG-1004 25 reactions
insights into how these cells function.
– Up to 70% transfection efficiency
– Up to 90% viability
– Transgene expression for several days A B
– Differentiation into neurons and astrocytes possible
The use of Rat NSCs in research will lead to a better Ordering Information
understanding of normal brain development. Additionally,
Cat. No. Size Price
neural cells derived from NSCs have the ability to replace
damaged neurons or degenerate tissue. The Nucleofector® Rat NSC (Neural Stem Cell) Nucleofector® Kit
Technology offers the first non-viral method for efficient
gene transfer into primary neural stem cells. VPG-1005 25 reactions
Nucleofection® of Rat NSCs. Primary NSCs isolated from rat embryos (E14)
were transfected by Nucleofection® using the Rat NSC Nucleofector® Kit,
program A-31/A-031 and a plasmid encoding enhanced green fluorescent
protein eGFP under control of an EF1alpha promoter (pcDNAEF1-eGFP).
Post Nucleofection®, cells were cultured with bFGF for 2 days, then for
5 additional days without bFGF to differentiate into neurons. Cells were
analyzed 2 days (A) and 7 days (B) post Nucleofection® by fluorescence
microscopy.
(Photograph courtesy of S.H. Lee, College of Medicine, Dept. of Biochemistry,
Hanyang University, Seoul, South Korea.)
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Rat Cardiomyocyte – Neonatal Nucleofector® Kit
Rat Cardiomyocytes
Nucleofector® Kits
A B
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Human Chondrocyte Nucleofector® Kit
Human Chondrocytes
Nucleofector® Kits
The study of chondrocytes, the matrix-forming cells of Ordering Information
cartilage, is of major importance in tissue engineering and
Cat. No. Size Price
regenerative medicine. Nucleofection® of chondrocytes
allows for a better understanding of the basic principles Human Chondrocyte Nucleofector® Kit
of degenerative processes such as osteoarthritis.
VPF-1001 25 reactions
– Up to 65% transfection efficiency
– First efficient non-viral transfection technology
A B
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MEF (Mouse Embryonic Fibroblast) Nucleofector® Kit
Nucleofector® Kits
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Optimized Protocols for Cell Lines
Optimized Protocols
for Cell Lines
NF: An Optimized Protocol is available using the Cell Type Optimized Transfection Viability
Amaxa® Nucleofector® Device Protocol Efficiency
96: An Optimized Protocol is available using the 293 NF 84%
Amaxa® 96-well Shuttle® System 293 96 83% 93%
32D NF 79% 61%
3T3-L1 ad NF 25% 90%
3T3-L1 pre-ad NF 73% 59%
A-10 NF 64% 74%
A-375 NF 72% 97%
A-431 NF 45% 83%
A20 NF 74% 81%
A2058 NF 81% 94%
A549 NF 72% 81%
A7r5 NF 49% 81%
AGS NF 73% 62%
ARPE-19 NF 83% 92%
ARPE-19 96 87%
B16-F0 NF 84% 91%
B16-F10 NF 91% 96%
BA/F3 NF 88% 79%
BHK-21 NF 85% 78%
BJ NF 52% 76%
BxPC-3 NF 28% 62%
C2C12 NF 82% 93%
C6 NF 70 – 94% 80%
Caco-2* NF 59% 70%
Capan-1 NF 29% 78%
CCRF-CEM NF 68% 79%
CHO [suspension] NF 84 – 92% 82%
CHO-K1 NF 94% 53 – 87%
CHO-K1 96 86% 97%
CHO-S [suspension] NF 90 – 98% 67 – 72%
CHO-S [suspension] 96 84 – 86% 55 – 57%
COS-1 NF 49% 64%
COS-7 NF 99% 94%
COS-7 96 91 – 99% 80 – 96%
D1 ORL UVA NF 61% 97%
DU 145 NF 47% 89%
EL4 NF 65% 76%
EL4 96 70 – 80%
FDC-P1 NF 82% 84%
GH3 NF 77% 84%
GH3 96 60 – 80% 60 – 70%
H9c2(2-1) NF 86% 90%
HaCaT NF 43%
HCT 116 NF 78% 76%
HCT 116 96 70 – 80% 65 – 75%
*Caco-2 was developed by the Memorial Sloan Kettering Cancer Center
and is accordingly subject to certain third party rights and commercial HeLa NF 70%
use restrictions. Please contact Memorial Sloan Kettering Cancer Center or HeLa 96 75% 89%
Navicyte Scientific for licensing information.
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Optimized Protocols for Cell Lines
Optimized Protocols
Continued
for Cell Lines
NF: An Optimized Protocol is available using the Cell Type Optimized Transfection Viability
Amaxa® Nucleofector® Device Protocol Efficiency
96: An Optimized Protocol is available using the Neuro-2a [N2a] NF 76% 79%
Amaxa® 96-well Shuttle® System Neuro-2a [N2a] 96 67% 82%
NG108-15 NF 64% 82%
NIH/3T3 NF 84% 87%
NIH/3T3 96 95% 93%
NK-92 NF 26% 40%
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Nucleofection® of Cell Lines
Nucleofection® of
Cell Lines
For the transfection of cell lines, Lonza offers five different Ordering Information
Amaxa® Cell Line Nucleofector® Solutions: C, L, R, T, and V.
Cat. No. Size Price
Optimized Protocols outlining the optimal Nucleofector®
Kit for a large selection of cell lines are already available Cell Line Nucleofector® Kit C
and can be downloaded from www.lonza.com/celldatabase.
VCA-1004 25 reactions
Lonza’s R&D Team is continuously optimizing the
Nucleofection® Protocols for new cell. Make sure you are Cell Line Nucleofector® Kit L
up-to-date with our latest developments by visiting our
VCA-1005 25 reactions
cell line database.
– Achieve transfection efficiencies of up to 90% with Cell Line Nucleofector® Kit R
high cell viability VCA-1001 25 reactions
– Up to 99% transfection efficiency with siRNA duplexes Cell Line Nucleofector® Kit T
even in suspension cells
VCA-1002 25 reactions
– Expression within hours - from transfection to
analysis in a day Cell Line Nucleofector® Kit V
VCA-1003 25 reactions
For the high throughput transfection of cell lines using Ordering Information
the 96-well Shuttle® System, Lonza offers three different
Cat. No. Size Price
Amaxa® Cell Line 96-well Nucleofector® Kits: SE, SF
and SG which are available in two different sizes (96 Cell Line 96-well Nucleofector® Kit SE
and 960 reactions). Optimized Protocols outlining the
VHCA-1001 96 reactions
optimal Nucleofector® Kit for a selection of cell lines
VHCA-2001 960 reactions
are already available and can be downloaded from
www.lonza.com/celldatabase. Lonza’s R&D Team is Cell Line 96-well Nucleofector® Kit SF
continuously optimizing the Nucleofection® Protocols
VHCA-1002 96 reactions
for new cell lines. Make sure you are up-to-date with
VHCA-2002 960 reactions
our latest development by visiting our cell database.
– High throughput transfection with excellent Cell Line 96-well Nucleofector® Kit SG
performance and high reliability VHCA-1003 96 reactions
– siRNA and shRNA screening in cell lines VHCA-2003 960 reactions
with high medical relevance
– High throughput protein expression in
suspension cell lines
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Cell Line Optimization Nucleofector® Kit
Nucleofection® of
Cell Lines
1 Solution L V 2 Solution V
Related Products
Classical Media 251 – 260
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Cell Line Optimization 96-well Nucleofector® Kit
Nucleofection® of
Cell Lines
With the unique capability of the 96-well Shuttle® System to Ordering Information
address up to 96 different programs in one Nucleocuvette®
Cat. No. Size Price
Plate, cell line optimizations are easily performed. The
Amaxa® Cell Line Optimization 96-well Nucleofector® Kit Cell Line Optimization 96-well Nucleofector® Kit
is the ideal tool to conveniently and rapidly determine
VHCO-1001 96 reactions
the optimal 96-well Nucleofection® Condition of virtually
any difficult-to-transfect cell line within one experiment.
31 different programs plus one control are tested
with the Cell Line 96-well Nucleofector® Solutions SE,
SF and SG in one 96-well Nucleocuvette® Plate. Once
the best Cell Line Nucleofector® Solution and program
have been determined, Lonza‘s Scientific Support Team
is available to assist you in improving the results even
further.
– Transfection of virtually any difficult-to-transfect
cell line
– Simple and rapid optimization within just one
experiment and plate
1 2 3 4 5 6 7 8 9 10 11 12
+
A
B 96-well Nucleofector ®
C Solution SF
D
3
> > >
E
F
G
H
Related Products
Classical Media 251 – 260
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cGMP Cell Line Nucleofector® Kits
Nucleofector® Kit
Basic Parasite
The Amaxa® cGMP Cell Line Nucleofector® Solutions enable Ordering Information
you to perform your transfection experiments under
Cat. No. Size Price
animal component-free conditions. They are, therefore,
the ideal tool for the Nucleofection® of cell lines used to cGMP Cell Line Nucleofector® Kit C
generate stable protein-producing clones for large-scale
VGA-1004 25 reactions
protein production.
– Efficient transfection of cell lines relevant to protein cGMP Cell Line Nucleofector® Kit L
production, e.g., CHO, suspension CHO, suspension
VGA-1005 25 reactions
293, NS0
– Non-animal-origin free formulation cGMP Cell Line Nucleofector® Kit R
– Production under cGMP conditions reduces
VGA-1001 25 reactions
regulatory concerns
– Certified for absence of DNA/RNA, DNase/RNase, cGMP Cell Line Nucleofector® Kit T
particles and endotoxins
VGA-1002 25 reactions
(e.g., in Plasmodium berghei and Trypanosoma brucei) Basic Parasite Nucleofector® Kit 1
compared to standard methods such as electroporation
VMI-1011 25 reactions
or particle bombardment. Due to significant genotypic
and phenotypic diversity between species and life Basic Parasite Nucleofector® Kit 2
cycles, Lonza has developed two Amaxa® Basic Parasite
VMI-1021 25 reactions
Nucleofector® Kits (1 and 2) and an easy-to-use Amaxa®
Basic Parasite Nucleofector® Starter Kit.
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FAQs on Nucleofection®
FAQs on Nucleofection®
Q. Why is the Amaxa® Nucleofector® Technology ideal this. It comes with different cuvettes designed for
for primary cells and difficult-to-transfect cell lines? 20 µl sample volume. With the Small Cell Number
A. There are several reasons to choose the Amaxa® Nucleofector® Kit 20,000-100,000 cells per sample
Nucleofector® Technology for your gene transfer are used.
experiments. One is the direct transfer of DNA For the 96-well Shuttle® System, the sample
into the cell nucleus. This makes gene expression volume is 20 µl (typically cutting the cell number
independent from cell division. Therefore, the recommended for the single cuvette by 1/5). The
technique is the ideal tool for primary cells, even lower limits are often dictated by the minimum
for non-dividing cells, such as neurons. In cell amount of cells required for optimal plating density
lines as well as primary cells, this yields gene on 96-well cell culture plates.
expression shortly after transfection and in many Q. When transfecting cancer cell lines, do I need to be
cases transfection efficiencies of over 50% can be concerned about passage number?
detected in as little as 2-4 hours. A. For the most efficient gene transfer, we recommend
Q. What optimization is necessary to get the technology using cells that are in logarithmic growth phase
to work? and at a passage number of 10-15 (from the time of
A. In most cases none. For each cell type in our pro- thaw). This is because some cell lines differentiate
duct list, we offer a cell-type-specific solution and and change their features after many passages.
thoroughly optimized electrical parameters. These Q. What is the optimal size of DNA that you recommend
are already pre-programmed in the Nucleofector® for Nucleofection®?
Device. Our Amaxa® Optimized Protocols give you A. We routinely use plasmids of 4-7 kb in our
recommendations regarding cell culture and laboratories and plasmids up to approximately
handling for optimum transfection results. 20 kb should not be a problem. The optimized
Q. What can I do when my cell of interest is not in your conditions are cell-type-specific. That means you
range of products? can use the identical set up for DNA, siRNA, mRNA,
A. Lonza is constantly developing new Amaxa® peptide and large molecules like bacterial artificial
Nucleofector® Kits and Optimized Protocols for an chromosomes (BACs) as well.
increasing number of primary cells and cell lines. It is very likely that you will see a decrease in the
In order to obtain the latest information, check our transient efficiency when BACs are used as a
website or contact our Scientific Support Team. substrate, but in general it works very well. You
If your target cell is a cell line, we offer the Cell Line also have to consider that a) by using 5-10 µg
Optimization Nucleofector® Kit. This kit enables DNA you have less copies in your experiment than
a rapid and simple determination of the optimal with standard constructs which leads to lower
Nucleofection® Conditions for your cell line of interest, brightness of the cells, and b) the quality of the
including those which are difficult-to-transfect. DNA is very important. So try to use endotoxin free
Q. What are your recommendations for minimum and DNA preparations, and check the DNA quality on an
maximum cell numbers for Nucleofection®? agarose gel to be sure that the DNA isn’t nicked.
A. The recommended cell number will vary depending
on which Amaxa® Optimized Protocol is being used.
In general for the Nucleofector® Device (single
cuvette format), using less than 2x105 cells per
reaction causes a major increase in cell mortality.
For some cell lines, we have tried cell numbers up
to 3x107. The problem here is that you end up with
a very large cell pellet leading to a dilution of the
Nucleofector® Solution as the maximum cuvette
volume is 100 µl to 110 µl. We suggest increasing
the cell number successively from experiment to
experiment but not exceeding a cell pellet volume
of 50 µl. Our Small Cell Number Nucleofector® Kit
for primary neurons represents an exception to
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FAQs on Nucleofection®
FAQs on Nucleofection®
Q. How do you determine cell death? By comparing the X-values for the untreated sample
A. We determine cell death in two ways: and the transfected samples you can calculate how
1) ToxiLight® Non-Destructive Cytotoxicity many dead cells you have in your treated samples.
BioAssay Kit. ToxiLight® is a patented luminescent Q. Does your system work for co-transfections of
assay that measures the release of adenylate plasmids?
kinase. Unequivocal results are available in less A. In principle, co-transfection of different plasmids
than 10 minutes. It can detect as few as 10 dead should not be a problem. However, the total amount
cells per well without requiring a lysis step. Please of DNA used is a crucial point because high amounts
see more details on page 226. of DNA could cause decreased cell viability. For the
2) Flow cytometry determination of viable/dead Nucleofector® Device (single cuvette format), we
cells by propidium iodide staining. We normally recommend starting co-transfection experiments
analyze transfection efficiency in living cells by flow with 1 µg - 5 µg total DNA first and then successively
cytometry: increasing the DNA amount if necessary.
We first exclude cellular debris by gating for the Also verify that both plasmids will be expressed in
"normal" population, (regarding size and granularity) this cell type. For the 96-well Shuttle® System you
in the forward-sidescatter. From this gated should calculate 1/5 of the amount; 0.2 µg - 1 µg
population we determine dying cells by propidium DNA per vector.
iodide staining and exclude them from analysis by Q Can I use the Amaxa® Nucleofector® Technology for
setting another gate. So, only those cells which are RNAi applications? How do I start?
in the FSC-SSC gate and are not propidium iodide A. Yes. The Amaxa® Nucleofector® Technology can
positive are analyzed for efficiency. be easily applied for any RNAi substrate (siRNA,
To be even more precise in the determination of shRNA, miRNA). You can use the same conditions
the mortality for adherent cells, we also collect the described in the cell-type-specific optimized
detached cells in the supernatant and combine these protocol for DNA vectors (cDNA, shRNA or miRNA
after trypsinization with the former adherent cells expressing plasmids) or oligonucleotides (siRNA,
and include them in the flow cytometry analysis. miRNA inhibtors).
This helps ensure that our data is complete and Prior to beginning comprehensive experiments,
accurate. multiple parameters associated with experimental
3) Flow cytometry determination of total cell loss design need to be optimized:
induced by Nucleofection® (optional method): Selection of appropriate controls (non-targeting
In order to get an idea about the total cell loss, we siRNA, siRNA targeting a house-keeping gene,
compare the initial cell number per sample with the untreated cells).
final cell number per sample. Determination of minimum effective siRNA amount
For that we use APC-labeled beads from BD for your target gene in your cell type of interest.
Biosciences (to detect APC your flow cytometry Determination of the optimal analysis time point
needs a 4 channel laser, because APC is detected on (kinetics of down-regulation depend on target gene,
channel 4). cell type and analysis level).
We prepare a stock of beads (1000 beads/µl in Identification of a suitable and robust analysis
PBS). After 5 minutes of vortexing, we add 50 µl of method (i.e. mRNA, protein or phenotypic analysis).
the beads to the sample we want to analyze by flow Q. Do you have any helpful calculations or conversion
cytometry. information for siRNA?
To count the beads by flow cytometry you have A. On our website, we provide a siRNA calculator
to lower the threshold in the FSC/SSC down to 20 which allows you to calculate from either amount to
because they are much smaller than cells. concentration or from concentration to amount and
After analysis, we do the following calculation to from either molar amount to weight or from weight
define the number of used living cells in the analyzed to molar amount.
sample (input X, unknown value):
input X = number of used beads*/counted beads x
counted cells in R2**
* input: 50 µl = 50000 beads
** R2 gate means: normal cell population in FSC/SSC without cellular
debris (gate R1) AND without PI-postive cells in FL-2/FL-3
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HiFect® Transfection Reagent
Transfection Reagent
HiFect®
The HiFect® Transfection Reagent is Lonza’s stand alone Ordering Information
biochemical transfection reagent for standard cell lines.
Cat. No. Size Price
While the well established Nucleofector® Technology
remains the best system for gene transfer into notoriously HiFect® Reagent
difficult-to-transfect cell lines and primary cells, the
VBA-1001 1 x 825 µl
HiFect® Transfection Reagent is the cost-effective
VBA-4001 4 x 825 µl
alternative for standard cell lines. As a company with
great expertise in transfection technology, Lonza prides
itself on having the ideal solution for any transfection
challenge.
– Superior transfection efficiencies and cell viabilities
– Cost effective
– Suitable for DNA and siRNA transfections
– Suitable for high throughput applications
– Scientific support by our trusted and experienced team
High Performance
Most transfection reagents are optimized for either high
efficiency or high cell viability. With Lonza's HiFect® High Efficiency Combined with Low Cytotoxicity
Transfection Reagent you can address both features You are working on a wide range of standard cell lines
equally. The data clearly show that using HiFect® and would like to have optimized transfection conditions
Reagent enables you to achieve superior efficiencies for all of them. With HiFect® Reagent – the transfection
and high viabilities in parallel, while cells maintain their reagent optimized by Lonza – you no longer have to
morphological and functional properties. keep several reagents in stock. Compared to other leading
transfection reagents, HiFect® Reagent ensures excellent
efficiencies and the lowest mortality rates.
A B
100 HiFect™
% Transfection efficiency
Competitor reagent L
Competitor reagent F
90
80
70
60
C 50
40
30
20
10
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pmaxFP® Fluorescent Protein Expression Vectors
Vectors
Lonza offers pmaxFP® Vectors expressing green, yellow and red fluorescent proteins.
– Monitor gene expression and protein localization in living mammalian cells
– Generate green, yellow or red fluorescent fusion proteins
– Study gene-regulation by using promoter-less (PRL) versions
Your benefits
■ Easy analysis
– Very bright green, yellow or red fluorescence
■ Non-toxic
– Suitable for transient and stable expression
■ Affordable
– Affordable pricing and license-free use for research purposes for for-profit entities
during the first six months after purchase
■ Flexible
– Various fusion constructs available; compatible with Nucleofector® Technology
A B C
HUVECs were transfected by Nucleofection® with 2 µg pmaxFP®-Green-C (A), pmaxFP®-Yellow-C (B) or pmaxFP®-Red-C
Vectors (C) and analyzed by fluorescence microscopy after 24 hours.
NOTE: pmaxFP® Vector products contain a proprietary nucleic acid coding for a proprietary fluorescent protein(s)
intended to be used for research purposes only. Any use of proprietary nucleic acid or fluorescent proteins coding by
proprietary nucleic acid other than for research use is strictly prohibited. USE IN ANY OTHER APPLICATION REQUIRES
LICENSE FROM EVROGEN. To obtain such a license, please contact Evrogen at license@evrogen.com.
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pmaxFP® Vector Backbone Information
Vectors
pmaxFP® Vectors for C-terminal Fusions
Immediate early promoter of cytomegalovirus (PCMV IE) for protein expression
SV40 origin for replication in mammalian cells
pUC origin of replication for propagation in E. coli
f1 origin for single-stranded DNA production
SV40 early promoter provides neomycin resistance gene expression to select stably transfected
eukaryotic cells using G418
Multiple cloning site (MCS) is located between maxFP®-Green, -Yellow or -Red Gene coding sequence and SV40
polyadenylation signal (SV40 poly A)
Multiple cloning site (MCS) is located between PCMV IE and maxFP®-Green, -Yellow or -Red Gene coding sequence
Multiple cloning site (MCS) is located upstream of maxFP®-Green, -Yellow or -Red Gene coding sequence
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pmaxFP®-Green Vectors
Vectors
pmaxFP®-Green Vectors are eukaryotic (mammalian) maxFP®-Green Gene with humanized codon usage, i.e., it is
expression vectors encoding green fluorescent protein optimized for high expression in mammalian cells.
maxFP®-Green, an improved maxGFP® Reporter Protein – Extra fast protein maturation allowing early signal
variant from copepod Pontellina plumata. Different detection
from maxGFP® Reporter Protein, which is expressed by
our positive control pmaxGFP® Vector (provided in all – Bright green fluorescence, easy-to-detect
Nucleofector® Kits), maxFP®-Green Reporter Protein is – No aggregation, good performance in fusions
suitable for stable expression and generation of fusion – Suitable for stable expression
proteins. pmaxFP®-Green Vectors carry a synthetic
pmaxFP®-Green-C Vector
VDF-1011 20 µg
*not unique site.
sites are blocked by methylation. If you
Concentration: 0.5 µg/µl
wish to digest the vector using these sites,
you will need to transform the vector into a
dam- host and make fresh DNA.
Use:
– Generation of fusions to the C-terminus of the maxFP®-Green Reporter
Protein for expression and localization studies
– Expression of maxFP®-Green Reporter Protein in mammalian cells
pmaxFP®-Green-N Vector
VDF-1012 20 µg
Concentration: 0.5 µg/µl
pmaxFP®-Green-PRL Vector
VDF-1013 20 µg
Concentration: 0.5 µg/µl
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pmaxFP®-Yellow Vectors
Vectors
pmaxFP®-Yellow Vectors are eukaryotic (mammalian) generation of fusion to its C-terminus was produced
expression vectors encoding fluorescent maxFP®-Yellow by random mutagenesis of maxFP®-Yellow Reporter
or maxFP®-Yellow-m Reporter Proteins, respectively. Protein. pmaxFP®-Yellow Vectors carry maxFP®-Yellow and
maxFP®-Yellow Reporter Protein is a mutant of a natural maxFP®-Yellow-m Genes with humanized codon usage.
yellow fluorescent protein from jellyfish Phialidium sp. – No aggregation, good performance in fusions
However, maxFP®-Yellow Reporter Protein was found to
be inappropriate for generating fusions to its C-terminus – maxFP®-Yellow-m: for C-terminal fusions
due to folding problems. To overcome this problem, – maxFP®-Yellow: for N-terminal fusions
maxFP®-Yellow-m Reporter Protein mutant suitable for
pmaxFP®-Yellow-C Vector
VDF-1021 20 µg
*not unique site.
# sites are blocked by methylation. If you
Concentration: 0.5 µg/µl
wish to digest the vector using these sites,
you will need to transform the vector into a
dam- host and make fresh DNA.
Use:
– Expression of maxFP®-Yellow-m Reporter Protein in mammalian cells
– Generation of fusions to the C-terminus of maxFP®-Yellow-m Reporter
Protein for expression, localization and cellular dynamic studies
pmaxFP®-Yellow-N Vector
VDF-1022 20 µg
Concentration: 0.5 µg/µl
pmaxFP®-Yellow-PRL Vector
VDF-1023 20 µg
Concentration: 0.5 µg/µl
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pmaxFP®-Red Vectors
Vectors
pmaxFP®-Red Vectors are eukaryotic (mammalian) – True red fluorescence (no interference with green or
expression vectors carrying maxFP®-Red Gene with yellow fluorescence)
humanized codon usage. maxFP®-Red Reporter Protein is – No aggregation, good performance in fusions
a novel red fluorescent protein obtained by mutagenesis
of an Anthomedusae jellyfish chromoprotein. – Suitable for stable expression
pmaxFP®-Red-C Vector
VDF-1031 20 µg
Concentration: 0.5 µg/µl
# sites are blocked by methylation. If you
wish to digest the vector using these sites,
you will need to transform the vector into a Use:
dam- host and make fresh DNA.
– Expression of maxFP®-Red Reporter Protein in mammalian cells
– Generation of fusions to the C-terminus of maxFP®-Red Reporter Protein
for expression and localization studies, protein-protein interaction and
co-localization studies
pmaxFP®-Red-N Vector
VDF-1032 20 µg
Concentration: 0.5 µg/µl
Use:
– Expression of maxFP®-Red Reporter Protein in mammalian cells
– Generation of fusions to the N-terminus of maxFP®-Red Reporter Protein
for expression and localization studies, protein-protein interaction and
co-localization studies
pmaxFP®-Red-PRL Vector
VDF-1033 20 µg
Concentration: 0.5 µg/µl
Use:
– Promoter studies in mammalian cells
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pmaxCloning™ Vector
Vectors
– High expression rates in mammalian cells Ordering Information
– License-free use for research purposes Cat. No. Size Price
– Multiple cloning site for convenient insertion of the
pmaxCloning™ Vector
gene-of-interest
VDC-1040 20 µg
Concentration: 0.5 µg/µl
pmaxCloning™ Vector
NOTE: The CMV promoter is covered under the U.S. patents 5,168,062 and
5,385,839 and its use is permitted for research purposes only. Any other
use of the CMV promoter requires a license from the University of Iowa
Research Foundation, 214 Technology Innovation Center, Iowa City, IA.
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MycoZap™ Mycoplasma Elimination Reagent
MycoZap™
Reports are that 5 - 35% of all cells in continuous culture are Ordering Information
contaminated with mycoplasma. A cell line infection can Cat. No. Size Price
affect every known process in the cell and can seriously
impact the reliability, reproducibility and consistency MycoZap™ Mycoplasma Elimination Reagent
of results. Additionally, mycoplasma infections can LT07-818 1 treatment
cells. 10000
– Mycoplasma elimination 10
100000
10000
2°C – 8°C 1
100
No mycoplasma detected
10
198
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Antibiotics and Antimycotics
and Antimycotics
Antibiotics
Ordering Information Ordering Information
Cat. No. Size Price Cat. No. Size Price
17-745H 20 ml
17-745E 100 ml
Penicillin-Streptomycin-L-glutamine Mixture
contains 25,000 units potassium penicillin, 25,000 µg streptomycin sulfate,
and 29.2 mg L-glutamine per ml
Storage Conditions: -10°C – -20°C
17-718R 25 × 4.5 ml
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siRNA Test Kit
siRNA Test Kit
Establish your siRNA experiments faster than ever before. Ordering Information
The siRNA Test Kit is based on co-transfection of the
Cat. No. Size Price
pmaxGFP® Vector and an siRNA duplex directed against
maxGFP® Reporter Protein. Knock-down of maxGFP® siRNA Test Kit
Reporter Protein expression can be easily detected
VSC-1001 25 - 50 reactions
by fluorescence microscopy. The kit has already been
evaluated in multiple cell types such as Jurkat, THP-1,
U-937, NIH/3T3, or primary NHDF cells. It is not suitable for
use in primary human blood cells.
– Easy set-up of siRNA transfections
– Suitable for cell lines and primary adherent cells
0.5 μg 1 μg 2 μg
A B C
D E F
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Peptide Transfection Control
Transfection Control
Nucleofection® for Peptide Transfection
Peptide
Start Nucleofection® of peptides quickly and easily. The Ordering Information
Amaxa® Peptide Transfection Control offers a simple
Cat. No. Size Price
method to control the peptide transfection process
when used together with your regular cell-type specific Peptide Transfection Control
Nucleofector® or 96-well Shuttle® Kits. The fluorescently
VFP-1001 50 µg (lyophilized)
labeled (FAM) control peptide is visible in the cytoplasm up
to several hours following transfection and shows that the
Nucleofector® Program enabled delivery of extracellular
cargos, such as your experimental peptides.
– Easily control peptide transfection optimizations
– Explore peptide function with direct delivery into the
cytoplasm
– Analyze active protein pathways with the same
program as for DNA and RNA
Bright field and fluorescence images of SH-SY5Y cells 2 hours after Nucleofection® with the Amaxa® Peptide Transfection Control
at the given concentrations. Cells show ‘normal’ morphology and intracellular distribution of the fluorescently labeled (FAM)
peptideis homogenous.
(Image courtesy of Prof. R.Brock, Dept. of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Netherlands.)
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Transport™ Protein Delivery Reagent
Transfection Reagents
■ Storage Conditions
-20°C
TransportTM Reagent
Company A Company B were used to transfect cells with β-Galactosidase. Note higher
3.5
activity of cells transfected with Transport™ Reagent and absence of
precipitation.
3
2.5
■ Simple Protocol
1.5
Combine Transport™ Reagent with your
1 molecule, incubate at room temperature
for 15 – 30 minutes, then add to your
0.5 cells.
0 NIH3T3 HUVEC NHBE AoSMC
Cell Type
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PrimeFect™ DNA and siRNA Transfection Reagents
Transfection Reagents
Optimized for delivery of DNA and siRNA into primary cells
— Low cytotoxicity – Maintain cell density and obtain a PrimeFect™ Dilution Buffer
higher yield of transfections PA-3271 50 ml
— Transfection in the presence or absence of serum –
Does not require media changes and can be carried
out in serum containing media
■ Storage Conditions
2°C – 8°C, do not freeze
■ Applications
– Plasmid DNA delivery
– siRNA delivery
Recommended PrimeFect™ Transfection Reagents for A comparison of PrimeFect™ I Reagent with other leading
Clonetics® and Poietics® Primary Human Cells transfection reagents
80
% of Transfected Cells
Company I
Plasmid DNA siRNA Company R
Clonetics® or Poietics® 70
PrimeFectTM I
Primary Cell Type PrimeFect™ I PrimeFect™ II PrimeFect™ siRNA
60
Human Umbilical Vein
■ ■
Endothelial Cells – HUVEC 50
Human Blood
40
Microvascular Endothelial ■ ■
Cells – HMVEC-dBl 30
Human Mammary
■ ■ 20
Epithelial Cells – HMEC
Human Mesenchymal Stem 10
■ ■
Cells – HMSC
0 AoSMC SkMC HMVEC-dBI HMEC NHEK
Human Coronary Artery Primary Cell Type
■ ■
Endothelial Cells – HCAEC
Plasmid DNA was complexed with reagent and applied to 50 – 80%
Normal Human Epidermal
Keratinocytes – NHEK
■ ■ confluent monolayers in 6-well tissue culture dishes in accordance with
the manufacturer’s instructions. Cells were harvested 48 hours post-
Human Aortic Endothelial transfection and quantitated by flow cytometry.
■ ■
Cells – HAEC
Human Aortic Smooth AoSMC – Human Aortic Smooth Muscle Cells, SkMC – Human Skeletal Muscle
■ ■
Muscle Cells – AoSMC Cells, HMVEC-dBl – Human Blood Microvascular Endothelial Cells, HMEC
Human Skeletal Muscle – Human Mammary Epithelial Cells, NHEK – Normal Human Epidermal
Cells – SkMC
■ ■ Keratinocytes.
Normal Human Bronchial
■ ■
Epithelial Cells – NHBE
Human Prostate Epithelial
■ ■
Cells – PrEC
Related Products
Clonetics® Primary Cells and Media 2 –79
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PrimeFect™ DNA and siRNA Transfection Reagents
Transfection Reagents
Continued
A reporter plasmid expressing green fluorescent protein DNA (1μg) was transfected into HMVEC-d Bl - Human Blood Microvascular Endothelial Cells using
PrimeFect™ I and HAEC - Human Aortic Endothelial Cells using PrimeFect™ II. Cells were photographed and analyzed by flow cytometry at 48 hours post-transfection.
Cy3-labelled siRNA (25 nM final conc.) was transfected into HMEC - Human Mammary Epithelial Cells using PrimeFect™ siRNA. Cells were photographed and analyzed
by flow cytometry at 24 hours post-transfection. (All images are 10X magnification).
NOTE: PrimeFect™ I: Use of this product is covered under patents and patents pending. This product is sold under license from Synvolux Therapeutics BV and its
use is limited solely to research purposes.
NOTE: PrimeFect™ II or PrimeFect™ siRNA: Use of this product is covered under patents and patents pending. This product is sold under license from Mirus Bio
Corporation and its use is limited solely to research purposes.
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Transport™ Reagents FAQ's
Reagents FAQs
Transport™
Q. What type of studies would one do with Transport™
Protein Delivery Reagent?
A. Studies include understanding interactions of
proteins within the cell, using inhibitors to study
signal transduction, effects of transient over-
expression of proteins, and screening peptide
libraries.
Q. What cells have been transfected using Transport™
Reagent?
A. Several cell lines and primary cells have been
successfully transfected with Transport™ Reagent,
including:
– Human bronchial epithelial cells
– Human epidermal keratinocytes
– Human aortic smooth muscle cells
– HUVEC
– Human dermal fibroblasts
– Human neural progenitors
– NIH3T3
– 293
– C2C12
– CHO
– Rat hepatocytes
– Rat hippocampal/cortical neurons
Q. What happens to the Transport™ Reagent-Cargo
complex after it enters the cell?
A. After entering the cell, the disulfide bond
connecting Transport™ Reagent to the cargo
molecule is reduced due to the intracellular
environment and the complex dissociates.
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Notes
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Arrays
Chapter 3
StellARray™ Gene Expression Systems
Expression Systems
StellARray™ Gene
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StellARray™ Gene Expression Systems
Expression Systems
StellARray™ Gene
A fundamental hinderance to the advancement of basic Arrays can come in the form of tissues from several donors
research and drug discovery is the availability of tools for a specific disease and the respective normal margin
that allow simple comparisons of “like” things in one tissue on one plate. It can take the form of oligonucleotide
experiment. Our challenge is to deliver arrays of hard- sequences relevant to a specific genome bound to a
to-source components that, when seen together in one solid support. In the case of StellARray™ qPCR Arrays,
experiment, deliver valuable context to the researcher. PCR primer sets of genes relevant to a specific disease or
cell state are offered on one 96-well or 384-well plate for
real-time PCR gene expression analysis.
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StellARray™ Gene Expression Systems
Expression Systems
StellARray™ Gene
GeneSieve™ Query Single gene normalized fold change values for three
commonly used ‘normalizers’, sorted with respect to GPR
Challenging Dogma – Your cells are telling you more than ranking
you realize. 400
Fold Change
18s Normalizer
GAPDH Normalizer
B2M Normalizer
300
those small changes represent noise and are not relevant. 100
In actuality, small changes in expression of key genes can
significantly impact metabolic pathways. Consequently, 0
CD83
IGHA1
BLK
CD79A
LRMP
IL21R
IGHD
CD80
CD79B
FOSB
FOS
BCL11A
REL
LMO2
CD40
POU2AF1
PTPRC
TNRSF13C
EZH2
DTX1
CD19
LEF1
RAD51
IKZF1
IL8
CD86
IL2RA
TRAF4
PAX5
LTB
Fundamentally, researchers have operated under two
axioms when measuring gene or protein expression. Axiom 1. The top 30 (of 75) GPR-ranked genes are shown. Fold change values were
calculated using the ddCt method. The data was derived from the Lonza Human
Lymphoma and Leukemia 384 StellARray™ qPCR Array (Cat. No. 00188333) deter-
Axiom 1. You can predict a priori which gene is invariant mining the expression level changes in cDNA derived from Human Lymphoma
and thus useful as a normalizer. Yet, the expression levels compared to Normal Adjacent Tissue RNA (Applied Biosystems, Inc., Part No.
AM7268).
of all genes change at some time and there is normalizer Note: The varying profiles represent single gene normalizer bias. GPR ranks the
drift. So how can you select the best normalizer? statistical differences between sample groups AND THEN calculates a fold change
value. The ranking is NOT based on the magnitude of fold change.
By using the logic of pre-selecting normalizers, your lab Global Pattern Recognition™ (GPR) fold change values
could miss significant expression changes that potentially 80
Statistically Ranked Fold Change
GPR Analysis
40
StellARray™ Product
– Return a list of biological pathways and related Axiom 2. The top 30 (of 75) GPR-ranked genes are shown. Fold change values were
StellARray™ qPCR Arrays calculated using the GPR multi-normalizer method. The data was derived from
the Lonza Human Lymphoma and Leukemia 384 StellARray™ qPCR Array (Cat. No.
– Type in a disease name or gene and see relevant 00188333) determining the expression level changes in cDNA derived from Human
Lymphoma compared to Normal Adjacent Tissue RNA (Applied Biosystems, Inc.,
arrays in seconds Part No. AM7268). The ranking via GPR reveals the true profile without normaliza-
tion-based or fold change-based bias.
Note: The GPR statistically ranked list reveals a different profile from the Normalizer/
Fold Change-biased profile. GPR reveals a profile with fold change values asymptoti-
cally approaching the cells’ real state.
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StellARray™ Gene Expression Systems
Expression Systems
Continued
StellARray™ Gene
n StellARray™ qPCR Arrays n Global Pattern Recognition™ (GPR) Analysis Tool
– Primers arranged in 96-well and 384-well formats – Analyze data in minutes without housekeeping
for real-time PCR genes
– Determine gene expression levels or genomic DNA – Allows for quick and reliable analysis of real-tme PCR
copy number data
– Screen an entire biological pathway in one run – Returns a ranked gene list based on p-values
– Each 96-well StellARray™ qPCR Array contains – Normalizers determined by the experiment, not
94 gene-specific PCR primer pairs, plus genomic and a priori by a scientist
RNA controls
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StellARray™ Gene Expression Systems
Expression Systems
Continued
StellARray™ Gene
Electron Transport Toxicology 96 StellARray™qPCR Array Immune System Phenotyping 96 StellARray™ qPCR Array
00188220 2 x 96-well plates 00188237 2 x 96-well plates
00188266 2 x Fast 96-well plates 00188273 2 x Fast 96-well plates
Electron Transport Toxicology 4 x 96 StellARray™ qPCR Array Immune System Phenotyping 4 x 96 StellARray™ qPCR Array
00188302 2 x 384-well plates 00188309 2 x 384-well plates
FoxP3 Target Genes 96 StellARray™ qPCR Array Immunology 384 StellARray™ qPCR Array
00188231 2 x 96-well plates 00188332 2 x 384-well plates
00188267 2 x Fast 96-well plates
Immunological Targets of NFKB 96 StellARray™ qPCR Array
FoxP3 Target Genes 4 x 96 StellARray™ qPCR Array
00188238 2 x 96-well plates
00188303 2 x 384-well plates
00188274 2 x Fast 96-well plates
General Toxicology 96 StellARray™ qPCR Array Immunological Targets of NFKB 4 x 96 StellARray™
00188232 2 x 96-well plates qPCR Array
00188268 2 x Fast 96-well plates 00188310 2 x 384-well plates
General Toxicology 384 StellARray™ qPCR Array Innate Immune Signaling 96 StellARray™ qPCR Array
00188331 2 x 384-well plates
00188239 2 x 96-well plates
General Toxicology 4 x 96 StellARray™ qPCR Array 00188275 2 x Fast 96-well plates
00188304 2 x 384-well plates
Innate Immune Signaling 4 x 96 StellARray™ qPCR Array
Growth and Development Tox 96 StellARray™ qPCR Array 00188311 2 x 384-well plates
00188233 2 x 96-well plates Interferon Signaling & Response 96 StellARray™ qPCR Array
00188269 2 x Fast 96-well plates 00188240 2 x 96-well plates
Growth and Development Tox 4 x 96 StellARray™ qPCR Array 00188276 2 x Fast 96-well plates
00188305 2 x 384-well plates Interferon Signaling & Response 4 x 96 StellARray™
Hematopoetic Cell Lineage 96 StellARray™ qPCR Array qPCR Array
00188234 2 x 96-well plates 00188312 2 x 384-well plates
00188270 2 x Fast 96-well plates Lymphoma and Leukemia 384 StellARray™ qPCR Array
Hematopoetic Cell Lineage 4 x 96 StellARray™ qPCR Array 00188333 2 x 384-well plates
00188306 2 x 384-well plates
Mood Disorder 96 StellARray™ qPCR Array
Huntingtons Disease 96 StellARray™ qPCR Array 00188241 2 x 96-well plates
00188235 2 x 96-well plates 00188277 2 x Fast 96-well plates
00188271 2 x Fast 96-well plates
Mood Disorder 4 x 96 StellARray™ qPCR Array
Huntingtons Disease 4 x 96 StellARray™ qPCR Array 00188313 2 x 384-well plates
00188307 2 x 384-well plates
Mood Disorder 384 StellARray™ qPCR Array
00188334 2 x 384-well plates
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StellARray™ Gene Expression Systems
Expression Systems
Continued
StellARray™ Gene
Human Gene StellARray™ qPCR Arrays
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StellARray™ Gene Expression Systems
Expression Systems
Continued
StellARray™ Gene
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StellARray™ Gene Expression Systems
Expression Systems
Continued
StellARray™ Gene
Mouse Gene StellARray™ qPCR Arrays
Growth and Development Tox 96 StellARray™ qPCR Array Interferon Signaling & Response 96 StellARray™ qPCR Array
00188164 2 x 96-well plates 00188171 2 x 96-well plates
00188378 2 x Fast 96-well plates 00188385 2 x Fast 96-well plates
Growth and Development Tox 4 x 96 StellARray™ qPCR Array Interferon Signaling & Response 4 x 96 StellARray™
00188437 2 x 384-well plates
qPCR Array
00188444 2 x Fast 96-well plates
Hematopoetic Cell Lineage 96 StellARray™ qPCR Array
00188165 2 x 96-well plates Lymphoma and Leukemia 384 StellARray™ qPCR Array
00188379 2 x Fast 96-well plates 00188203 2 x 384-well plates
Hematopoetic Cell Lineage 4 x 96 StellARray™ qPCR Array Mood Disorder 96 StellARray™ qPCR Array
00188438 2 x 384-well plates 00188172 2 x 96-well plates
00188386 2 x Fast 96-well plates
Huntingtons Disease 96 StellARray™ qPCR Array
00188166 2 x 96-well plates Mood Disorder 4 x 96 StellARray™ qPCR Array
00188380 2 x Fast 96-well plates 00188445 2 x 384-well plates
Huntingtons Disease 4 x 96 StellARray™ qPCR Array Mood Disorder 384 StellARray™ qPCR Array
00188439 2 x 384-well plates 00188204 2 x 384-well plates
Immune Complement 96 StellARray™ qPCR Array Natural Killer Cell 96 StellARray™ qPCR Array
00188381 2 x Fast 96-well plates 00188173 2 x 96-well plates
00188167 2 x 96-well plates 00188387 2 x Fast 96-well plates
Immune Complement 4 x 96 StellARray™ qPCR Array Natural Killer Cell 4 x 96 StellARray™ qPCR Array
00188440 2 x 384-well plates 00188446 2 x 384-well plates
Immune System Phenotyping 96 StellARray™ qPCR Array Neurodegeneration 96 StellARray™ qPCR Array
00188168 2 x 96-well plates 00188174 2 x 96-well plates
00188382 2 x Fast 96-well plates 00188388 2 x Fast 96-well plates
Immune System Phenotyping 4 x 96 StellARray™ qPCR Array Neurodegeneration 4 x 96 StellARray™ qPCR Array
00188441 2 x 384-well plates 00188447 2 x 384-well plates
Immunology 384 StellARray™ qPCR Array NFKB Signaling 96 StellARray™ qPCR Array
00188202 2 x 384-well plates 00188183 2 x 96-well plates
00188389 2 x Fast 96-well plates
Immunological Targets of NFKB 96 StellARray™ qPCR Array
NFKB Signaling 4 x 96 StellARray™ qPCR Array
00188169 2 x 96-well plates
00188448 2 x 384-well plates
00188383 2 x Fast 96-well plates
Immunological Targets of NFKB 4 x 96 StellARray™ qPCR Notch Signaling 96 StellARray™ qPCR Array
Array 00188184 2 x 96-well plates
00188442 2 x 384-well plates 00188390 2 x Fast 96-well plates
Notch Signaling 4 x 96 StellARray™ qPCR Array
Innate Immune Signaling 96 StellARray™ qPCR Array
00188449 2 x 384-well plates
00188170 2 x 96-well plates
00188384 2 x Fast 96-well plates Obesity 96 StellARray™ qPCR Array
Innate Immune Signaling 4 x 96 StellARray™ qPCR Array 00188185 2 x 96-well plates
00188443 2 x 384-well plates 00188391 2 x Fast 96-well plates
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StellARray™ Gene Expression Systems
Expression Systems
Continued
StellARray™ Gene
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BioAssays
Chapter 4
BioAssays
New Products
OsteoImage™ Mineralization Assay 240
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BioAssays
BioAssays
When you need to perform high-throughput in vitro unprecedented performance. The bioassay product family
toxicology, drug discovery research, or assess the health provides an integrated assay platform for cell biology
of your cells, we have you covered. We offer a powerful line research that is complemented by our Clonetics® and
of products and services designed to deliver increased Poietics® Cells and Media. Assays are offered in kit format
speed, sensitivity, and convenience over conventional or can be supplied in bulk, thus providing appropriate
methods. Whether you’re studying cell proliferation, reagent quantities for each phase of the research or drug
apoptosis, cytotoxicity, cell differentiation, or enzyme discovery process.
activities, we have a line of bioassay kits that will deliver
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MycoAlert® Mycoplasma Detection Kit
Detect mycoplasma contamination in less than 20 minutes
The MycoAlert® Assay Control Set (sold separately) is Standard assay protocols were performed by an independent testing lab.
available with positive and negative controls for ensuring
proper assay performance. The control set does not
contain mycoplasma. ■ Applications
— Fast – Get results in less than 20 minutes to quickly – Mycoplasma contamination detection
assess the health of your cultures; other methods – Cell culture screening
take several hours to weeks to get results
— Convenient – Simply mix two reagents with 100 µl of ■ Storage Conditions
your culture supernatant and take two readings on 2°C – 8°C, do not freeze
your luminometer
— Universal – Detect all mycoplasma and acholeplasma Simple protocol
infections commonly contaminating cell cultures
— Sensitive – Detect <50 cfu/ml mycoplasma and avoid Cell culture supernatant (100 µl)
▼
false negative results often encountered with PCR and Add MycoAlert® Reagent
fluorescent staining procedures (Wait 5 min)
▼
— Complete – Each kit contains all of the reagents Measure luminescence
(Reading A)
needed to perform the assay, unlike commercial PCR ▼
kits, which require you to supply Taq Polymerase, Add MycoAlert® Substrate
(Incubate 10 min)
nucleotides, restriction enzymes, and agarose gel ▼
electrophoresis reagents (assay controls sold Measure luminescence
(Reading B)
separately) ▼
Ratio B/A >1 equals
MycoAlert® Kit is a mycoplasma detection assay. The assay and kits mycoplasma contamination
are covered by UK patent number GB2,357,336 and pending patent
applications.
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MycoAlert® Mycoplasma Detection Kit
Continued
Assay kinetics of light output for infected and uninfected cells Specifications
10,000
Luminescence (RLU)
MycoAlert® Mycoplasma
10
Detection Kit
Time(minutes) Liquid scintillation counter with
“luminescence” (i.e. out of coincidence)
mode.
Note: Absorbance readers, fluorometers, and ELISA plate readers are not
suitable instruments for the detection of bioluminescence. Check the
luminometer list at www.lonza.com for recommended luminometers.
Comparison of mycoplasma detection methods
Test Method
MycoAlert® Kit
PCR Amplification
Fluorescence
Staining
14-21 days
Culture Method
0 1 2 3 4 5 6
Time (hours)
Related Products
MycoZap™ Mycoplasma Elimination Agent 222
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MycoZap™ Mycoplasma Elimination Reagent
Reports are that 5 - 35% of all cells in continuous culture are Ordering Information
contaminated with mycoplasma. A cell line infection can
Cat. No. Size Price
affect every known process in the cell and can seriously
impact the reliability, reproducibility and consistency MycoZap™ Mycoplasma Elimination Reagent
of results. Additionally, mycoplasma infections can LT07-818 1 treatment
spread easily within the culture environment. To monitor LT07-918 5 treatments
for such infections, regular testing of cell lines should
be performed using Lonza’s MycoAlert® Mycoplasma Competitor 1 –
MycoZap™ Mycoplasma
Detection Kit. Where contamination has occurred, and the Effect on1 —
cell viability and and mycoplasma removal
Elimination Reagent
cells. 10000
– Mycoplasma elimination 10
100000
10000
2°C – 8°C 1
100
No mycoplasma detected
10
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ViaLight® Cell Proliferation and Cytotoxicity BioAssay Kits
Fast and accurate cell proliferation or cytotoxicity analysis
ViaLight® Plus Cell Proliferation and Cytotoxicity ViaLight® HS Cell Proliferation and Cytotoxicity
BioAssay Kit BioAssay Kit
LT07-211 500 tests
The ViaLight® Plus Cell Proliferation and Cytotoxicity LT07-111 1,000 tests
BioAssay Kit is designed to deliver high, stable LT07-311 10,000 tests
luminescent signals for an extended period of time, for
greater experimental design flexibility. The easy, two-step ViaLight® MDA Plus Microbial Proliferation and
assay is scalable for high throughput applications and Cytotoxicity Kit
can be run on a variety of luminometers or scintillation LT07-122 1,000 tests
counters. LT07-322 10,000 tests
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ViaLight® Cell Proliferation and Cytotoxicity BioAssay Kits
Continued
ViaLight® Cell Proliferation and Cytotoxicity BioAssay Kits ViaLight® Plus BioAssay Kit signal stability
are: 1,000,000
Luminescence (RLU)
Highly sensitive – The high sensitivity of the ViaLight®
100,000
Plus and HS BioAssay Kits enable lower seeding densities
to be used, reducing the high costs of culture media and 10,000
plastics that other assays require.
1,000
— Fast – Results from a complete 96-well plate are
available in <15 minutes (including the cell lysis step) 100
ViaLight® BioAssay Kits
– Cell viability studies 0% 0 20 40 60 80 100 120 140 160 180 200 220
Time (minutes)
– High-throughput screening ViaLight® Plus Competitor
■ Storage Conditions Light output with ViaLight® Plus BioAssay Kit and a competitive luminescent
assay kit with A549 cells was compared over time. The consistent light
2°C – 8°C, do not freeze output, 10-fold lower background and exceptional lysis capabilities of the
ViaLight® BioAssay Kit ensure superior results.
Background
Related Products
0.1 0.1 10 100 1,000 10,000 100,000
Cells/Well
ATP Standard 241
Light output at various times after reagent addition and with increasing Clear Bottom, White Walled Tissue Culture Plates 241
numbers of K562 cells demonstrate the exceptional sensitivity and dynamic
range delivered by the ViaLight® Plus BioAssay Kit. ExPro™ Luminometer Injector Cleaning Solution 241
ToxiLight® Non-destructive Cytotoxicity BioAssay Kit 226
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ViaLight® Cell Proliferation and Cytotoxicity BioAssay Kits
Continued
Specifications
Examples of cell types and treatments tested using ViaLight® BioAssay Kits
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ToxiLight® Non-destructive Cytotoxicity BioAssay Kit
Single-reagent, high content cytotoxicity assay
using the bioluminescent firefly luciferase reaction. As ToxiLight® 100% Lysis Control Set (sold separately)
the level of cytolysis increases, the amount of AK in the LT07-517 10 ml (200 tests)
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ToxiLight® Non-destructive Cytotoxicity BioAssay Kit
Continued
ViaLight® RLUs
ToxiLight® RLUs
60,000
Luminescence (RLU)
60,000
25,000
50,000
50,000
20,000
40,000 40,000
15,000
30,000
30,000
ToxiLight® Non-destructive
10,000
ToxiLight® BioAssay Kit is sensitive to 10 cells Adenylate kinase is stable over 3 days
120,000
RLUs
Luminescence (RLU)
100,000 7000
R2 = 0.9979
80,000 6000
60,000 5000
40,000 4000
20,000 3000
0 2000
0 10,000 20,000 30,000 40,000 50,000
Cells/Well
1000
Exceptional sensitivity and wide dynamic range results in exceptional
experimental flexibility.
0
Day 1 Day 2 Day 3
Days after lysate
Jurkat cells were seeded at 105 cells/ml and immediately lysed using
the ToxiLight® 100% Lysis Reagent. The stability of the released AK was
measured at 24h, 48h, and 72h after release with no significant loss in
activity.
Specifications
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ApoGlow® Rapid Apoptosis Screening Kit
Differentiate between apoptosis, necrosis and cell proliferation with a single assay
ApoGlow® Screening Kit is an apoptosis assay. The assay and kits are
covered by US patent number 6,004,767 and UK patent number
GB2,323,167.
0.35 60
% Apoptosis
Ratio
% Apoptosis (PI)
0.3 50
0.25 40
0.2 30
0.15 20
0.1 10
Dexamethasone (nM)
Related Products
Graph shows a comparison between ApoGlow® Screening Kit ratios and %
apoptosis determined by flow cytometric analysis of the sub G0/G1 peak ATP Standard 241
obtained with PI staining. CEM-7 cells were incubated with increasing Clear Bottom, White Walled Tissue Culture Plates 241
concentrations of dexamethasone for 72 hours. The data is shown as means
+/- SEM for 83 separate experiments. ExPro™ Luminometer Injector Cleaning Solution 241
ToxiLight® Non-destructive Cytotoxicity BioAssay Kit 226
ViaLight® Cell Proliferation and Cytotoxicity BioAssay Kits 223
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ApoGlow® Rapid Apoptosis Screening Kit
Continued
Specifications
Screening Kit
also be used, but sample throughput is compromised); microplate liquid scintillation counter with
“luminescence” (i.e. out of coincidence) model
Apoptotic Models Tested Using the ApoGlow® Rapid Apoptosis Screening Kit
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PKLight® HTS Protein Kinase Assay Kit
Rapid, generic assay for protein kinases
The PKLight® HTS Protein Kinase Assay Kit is designed Ordering Information
for rapid screening of protein kinase activity. The assay
Cat. No. Quantity Price
utilizes the bioluminescent measurement of ATP to provide
a generic endpoint detection system for determination PKLight® HTS Protein Kinase Assay Kit
of a wide range of protein kinases. The PKLight® Kit is LT07-500 500 tests
non-radioactive, homogeneous, robust and simple, and is LT07-501 5,000 tests
suitable for the screening of potentially all protein kinases 10 +
in 96-, 384- and 1536-well formats. Bulk reagents for high-throughput screening applications are also available.
PKLight® HTS Protein
Please Inquire.
Kinase Assay Kit
PKLight® Kit is a protein kinase assay. The assay and kits are covered by
UK patent number 2,375,171, US patent numbers 6,599,711 and 6,911,319,
Swiss patent number CH694,096, and pending patent applications.
Related Products
Clear Bottom, White-walled Tissue Culture Plates 241
ExPro™ Luminometer Injector Cleaning Solution 241
PDELight® HTS cAMP Phosphodiesterase Assay Kit 232
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PKLight® HTS Protein Kinase Assay Kit
Continued
PKLight® Assay results with protein kinase SAPK2a Kinase inhibitor (SB203580) inhibition of Kinase p38B
300,000 280,000
Luminescence (RLU)
Luminescence (RLU)
260,000
250,000
240,000
200,000 220,000
200,000
150,000
180,000
100,000 160,000
0 100,000
0 1.25 2.50 5.00 10.00 20.00 40.00 Neg 0 0.1 1 10 100 1,000 10,000
Control (SB203580) nM
(SAPK2 A) µg/ml
Concentration dependent consumption of ATP (starting concentration 10 100 ng of kinase was used to phosphorylate 10 µg of dephosphorylated
µM) by SAPK2α mediated phosphorylation of myelin basic protein (10 µg myelin basic protein in the presence of 10 µM ATP over a 1 hour incubation
in reaction mixture) as measured using the PKLight® HTS Protein Kinase period. A concentration dependent effect can clearly be observed using the
Assay Kit. assay. Western blot data clearly verifies the phosphorylation event.
Specifications
Number of tests per kit: 500 (500 assay points per 96- or 384-well plate)
5,000 (5,000 assay points per 96- or 384-well plate)
ATP range: <1 µM – 20 µM
Assay time: < 3 minutes per plate
Protein kinases: Serine/threonine, tyrosine, lipid kinases
Operating temperature: Ambient
Reproducibility: Typical Coefficient of Variation (CV) is <5%. Z´ Factor is >0.7
Recommended equipment: Microplate luminometer
Microplate liquid scintillation counter with “luminescence” (i.e. out of coincidence) mode
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PDELight® HTS cAMP Phosphodiesterase Assay Kit
Rapid, generic assay for cAMP dependent phosphodiesterases
the PDELight® Kit does not require costly radioactivity, – IC50 determinations
the use of beads, modified substrates, or antibodies.
■ Storage Conditions
The PDELight® Kit is ideally suited for primary and 2°C – 8°C, do not freeze
secondary screening of cAMP dependent phospho-
diesterases.
— Simple, homogeneous assay – A single reagent is Linear detection of AMP
added to the completed reaction and read 107
Luminescence (RLU)
r2 = 0.9992
— Generic platform – The same assay can be used for all
106
cAMP dependent phosphodiesterases
— Non-radioactive – The assay contains no hazardous 105 AMP
cAMP
reagents, eliminating costly disposal expenses
104
— Rapid assay – Complete a 384-well plate in less than
3 minutes 103
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PDELight® HTS cAMP Phosphodiesterase Assay Kit
Continued
Rapid screening and IC50 determinations with the PDELight® Assay Kit
100 1,000,000
% Inhibition
Luminescence (RLU)
MMPX
80 3-IBMX
IC50 = 6.2 µM
750,000
60
40 500,000
20
250,000
-20
0
Assay Kit
-40 0.01 0.1 1 10 100 1,000
Compounds
IBMX (µM)
Left Image. A library containing 150 pharmacologically active compounds was screened using the PDELight® Kit. The compounds MMPX and 3-IBMX were identified
as inhibiting phosphodiesterase activity greater than 50% at 10 µM. Right Image. An IC50 of 6.2 µM was determined for 3-IBMX.
Specifications
Number of tests per kit: 500 (500 assay points per 96- or 384-well plate)
AMP range: <10 nM – 20 µM
Assay time: < 3 minutes per plate
Phosphodiesterases: cAMP phosphodiesterases
Operating temperature: Ambient
Reproducibility: Typical Coefficient of Variation (CV) is <5%. Typical Z´ value > 0.8
Recommended equipment: Microplate luminometer
Microplate liquid scintillation counter with “luminescence” (i.e. out of coincidence) mode
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PPiLight® Inorganic Pyrophosphate Assay
formed from a number of different enzymatic reactions. PPiLight® Inorganic Pyrophosphate Assay
Enzymes that utilize PPi as a substrate may include LT07-610 500 tests
phosphotransferases (EC 2.7) and pyrophosphatases (EC
3.6.1.1). Enzymes that generate PPi are more numerous ■ Applications
and may include cyclases (EC 4.6.1), hydrolases (EC 3) – Measure activity of:
Pyrophosphate Assay
– Phosphotransferases
The PPiLight® Inorganic Pyrophosphate Assay is a – Pyrophosphatases
non-radioactive bioluminescent assay for the detection – Cyclases
of inorganic pyrophosphate. In the presence of PPi, the
detection reagent catalyses the conversion of AMP to – Ligases
ATP. The assay uses luciferase, which produces light from – Hydrolases
the newly formed ATP and luciferin following the reaction – DNA polymerases
schematic shown below.
■ Storage Conditions
— Fast – Measure enzyme activity via pyrophosphate
consumption in 1 hour 2°C – 8°C
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PPiLight® Inorganic Pyrophosphate Assay
Continued
Light produced directly proportional to PPi present Light increases proportionally to PPi concentration
8,000,000
RLUs
1,600,000
RLUs
6,000,000 1,200,000
4,000,000 800,000
300,000
200,000
0.156
Pyrophosphate Assay
2,000,000 100,000 400,000 0.313
PPiLight® Inorganic
PPi (µM)
0.625
0
0.0 0.1 0.2 0.3 0.4 1.25
Typical linear PPi standard curve using the PPiLight® Inorganic Pyrophosphate PPi signal increases over time at a steady, proportional rate as PPi
Assay. Sensitivity is typically 0.02 µM to 10 µM with r 2 values >0.95. concentration increases. Signal linearity is constant throughout a 1 hour
incubation.
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AdipoRed™ Assay Reagent
Quantify intracellular lipid accumulation
80,000
8,000
60,000
6,000
40,000
4,000
20,000
2,000
0 0 0 4 7 10
Day 0 Day 4 Day 4 Day 4
TNFA Mifeprestone Days of Induction
Poietics® Primary Human Preadipocytes were induced to differentiate in Poietics® Primary Human Preadipocytes were induced to differentiate and
the presence of TNFα, Mifepristone or no inhibitor. Lipid accumulation was assayed using the AdipoRed™ Assay Reagent.
assayed after 4 days in culture.
Related Products
Human Subcutaneous or Visceral Preadipocyte Cells 91
PGM™-2 Preadipocyte Growth Medium-2 BulletKit® 91
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OsteoAssay™ Human Bone Plate
Measure osteoclastic bone resorption
OsteoAssay™
EIA Kit
or animal bone slices 400
cell-based assays
— Novel – Contains real human bone for more biologically 100
relevant results
■ Applications 0
24 hr 48 hr 96 hr
– Bone resorption The release of collagen peptides from the OsteoAssay™ Plate by
differentiating primary human osteoclasts is linear with time. Poietics®
– Osteoclast precursor differentiation Osteoclast Precursors were seeded onto an OsteoAssay™ Plate at 10,000
cells/well and cultured in medium containing M-CSF +/- soluble RANK ligand.
– Osteoclast enzymatic activity After 5 days of culture, the medium was renewed. Samples of supernatant
were harvested after an additional 24, 48 and 96 hours and used in an EIA
■ Storage Conditions assay for a telopeptide.
-20°C
160
100
80
60
40
20
0 Differentiated Control
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OsteoLyse™ Assay Kit (Human Collagen)
Measure bone resorption in minutes
Human osteoclast activity measured by collagen release A comparison of TRAP and OsteoLyse™ Assay Kits in
using the OsteoLyse™ Assay Kit alendronate-mediated osteoclast function
1,200,000 250 1.6
Multinucleated TRAP-Positive Cells
Activity (RFU)
800,000 1.0
150
0.8
600,000
100
0.6
400,000 TRAP stain
OsteoLyse™ 0.4
50
200,000 0.2
0 0
0 0.12 0.23 0.47 0.94 1.88 3.75 8 15
0 Day 7 Day 8 Day 9 Day 10 Alendronate (mM)
Time in culture
Poietics® Primary Human Osteoclast Precursors were seeded onto an Poietics® Primary Human Osteoclast Precursors were seeded onto an
OsteoLyse™ Plate at 10,000 cells/well and differentiated with M-CSF and OsteoLyse™ Plate at 10,000 cells/well and differentiated with M-CSF and
soluble RANK ligand. At days 7, 8, 9 and 10 of culture, 10 µl of supernatant soluble RANK ligand in the presence of interferon γ. At day 10 of culture,
was removed and counted. The blue bars represent counts obtained when 10 µl of supernatant was removed and counted. The red line denotes TRAP
the precursors were cultured with M-CSF only. data (day 10 multinucleated TRAP-positive cells/well) while the green line
represents OsteoLyse™ Assay data.
Related Products
OCP Osteoclast Precursor BulletKit® 92
Human Osteoclast Precursors 92
CalciFluor™ Quantiative Bone Resorption Assay 239
OsteoLyse™ Assay Kit (Human Collagen) 238
OsteoImage™ Mineralization Assay 240
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CalciFluor™ Quantitative Bone Resorption Assay
Quantitative measurement of bone resorption
CalciFluor™ Quantitative
Bone Resorption Assay
CalciFluor™ Assay works well with human osteoclast
precursors and murine bone marrow mononuclear cells.
(Fig. 1a, 1b)
Poietics® Human Osteoclast Precursor Cells Murine Bone Marrow Mononuclear Cells
Figure 1a Figure 1b
1.4
Calcium (mM)
Calcium (mM)
control
differentiated
0.8 control 1.2
differentiated
1.0
0.6
0.8
0.4
0.6
0.2
0.4
0.2
0.0 8 9 10
Time of sampling (days)
0.0 11 12 13
Time of sampling (days)
-0.2
-0.2
Figure 1a. Human OCPs were seeded onto a human bone substrate at 10,000 Figure 1b. Murine BM-MNCs were seeded into a T-25 flask and cultured
cells/well and cultured with M-CSF +/- sRANKL. Medium was renewed on overnight. Nonadherent cells were harvested and seeded onto a human
day 7 and on days 8, 9, and 10 of culture, supernatants were removed and bone substrate at 50,000 cells/well and cultured with M-CSF +/- sRANKL.
assayed for calcium. Medium was renewed on days 5 and 10 of culture and on days 11, 12, and
13 supernatants were removed and assayed for calcium.
Related Products
OCP Osteoclast Precursor BulletKit® 92
Human Osteoclast Precursors 92
OsteoAssay™ Human Bone Plate 237
OsteoLyse™ Assay Kit (Human Collagen) 238
OsteoImage™ Mineralization Assay 240
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OsteoImage™ Mineralization Assay
Rapid, flourescent assay for bone mineralization
The OsteoImage™ Mineralization Assay is a rapid, The OsteoImage™ Mineralization Assay can quantitate
fluorescent, in vitro assay for assessing bone cell in vitro mineralization by osteogenic stem cells,
mineralization. The OsteoImage™ Assay can quantitate primary osteoblasts, and osteoblast-like cell lines
in vitro mineralization by osteogenic stem cells, primary (Figure 1). The assay is sufficiently sensitive to detect
osteoblasts, and osteoblast-like cell lines. It is based the time-dependent increases in mineralization in
on specific binding of the fluorescent OsteoImage™ differentiating osteoblast cultures (Figure 2).
Staining Reagent to the hydroxyapatite portion of bone-
like nodules deposited by cells. Works with stem cells, primary cells, and cell lines
Mineralization Assay
1,000
RFU (492/520)
Unlike typical histochemical methods such as von Kossa Undifferentiated
and Alizarin red, neither of which is hydroxyapatite Differentiated
OsteoImage™
800
specific, the OsteoImage™ Assay eliminates multiple
steps or tedious extraction steps. 600
Undifferentiated
stem cells, and osteoblast cell lines 500
Differentiated
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BioAssay Accessory Products
Clear Bottom, White Walled Tissue Culture Plates ExPro™ Luminometer Injector Cleaning Solution
Clear Bottom White Walled Tissue Culture Plates are white- ExPro™ Luminometer Injector Cleaning Solution is a
walled 96-well plastic plates designed specifically for use specially formulated detergent specifically designed for
with any bioluminescent bioassay kit. bioluminescent applications. ExPro™ Solution is intended
to clean injector lines (e.g., on luminometers and sample
Ordering Information processors) between running different luminescent
applications where there is a risk of transferring reagent
Cat. No. Size Price components between different assays. For example,
Clear Bottom, White Walled Tissue Culture Plates reagents used in ATP measurement (i.e., ViaLight®
LT27-102 25 plates BioAssay Kits) contain high levels of luciferase, which
BioAssay Accessory
can contaminate luciferase assays when used in reporter
gene analysis. ExPro™ Solution will neutralize the
Products
ATP Standard luciferase enzyme and prevent the risk of contaminating
one assay with reagents used in a different assay.
The ATP Standard is a specialized aqueous preparation of
adenosine triphosphate (ATP) and is primarily intended
NOTE: ExPro™ Solution has only been tested with luciferase-
for use in research to calibrate ATP assays based on the
containing assays. Performance is not guaranteed for
luciferase bioluminescence technique. Each vial contains
other applications.
5 ml of 10 µM ATP.
■ Storage Conditions
■ Storage Conditions
2°C – 8°C
-20°C
Ordering Information Ordering Information
Cat. No. Size Price Cat. No. Size Price
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Cell Analysis Reagents
Fluorescent reagents selected for optimal performance with Clonetics® and Poietics® Primary Cells
Phalloidin, Alexa Fluor® 488 Conjugate stains for F-actin MitoTracker™ Red CMxRos Mitochondrial Probe
at nanomolar concentrations. Labeled phalloidin has the PA-3017 5 × 50 µl
same affinity for large and small filaments, binding in a
stoichiometric ratio with one molecule per actin subunit
in muscle and nonmuscle cells from plants and animals.
It is suitable for labeling and identifying F-actin in tissue
sections or cell cultures.
■ Storage Conditions
-20°C, protect from light
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Cell Analysis Reagents
Continued
■ Storage Conditions
Vybrant® DiI Cell-Labeling Solution
-20°C, protect from light
PA-3020 1 ml
SYTOX® Green Nucleic Acid Stain Annexin V, Alexa Fluor® 488 Conjugate
SYTOX® Green is chemically similar to SYTO® Green but is PA-3021 125 µl (25 assays)
not cell permeant. SYTOX® Green may be used as a green
nuclear counterstain for multicolor fluorescence of fixed
cells or as a dead cell indicator in viability assays.
■ Storage Conditions
-20°C, protect from light
Differentiated NHNP stained live with Vybrant® Dil (red) and SYTO® Differentiated human skeletal muscle myoblasts stained with SYTOX®
Green Green
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G Protein-Coupled Receptors
Quality, quantity, quickly
G protein-coupled receptors (GPCRs) are a large super- sense that it is not known what endogenous molecules
family of cell-surface proteins whose function is to activate these receptors). They represent a tremendous
transduce information from extracellular space to opportunity to uncover novel ligands and novel therapeutic
the cell interior by stimulating (or inhibiting) second approaches to major diseases.
messenger systems. Included in this family are receptors
for neurotransmitters (such as receptors for dopamine, In the past few years, a new paradigm for drug discovery
acetylcholine, histamine, etc.) as well as receptors for has been elucidated, the “reverse pharmacological”
brain and gut peptides (e.g. bradykinin, gallanin, and approach to drug discovery. In a “normal” drug discovery
various neuropeptides). project, a target would be identified and validated as a
G Protein-Coupled
proper target for a particular therapeutic intervention,
GPCRs are an extremely important superfamily comprised then a screening program would be constructed around
Receptors
of some 800 different members. Drugs currently on the that target. In a reverse pharmacological approach, the
market that interact with GPCRs interact with only about screening of an orphan receptor precedes the target’s
100 out of the 800 identified GPCRs. Approximately 30% validation for a particular therapeutic intervention.
of all pharmaceuticals on the market today exert their
therapeutic effect by interacting with a GPCR. GPCRs still Many orphan GPCRs have been characterized in the past
represent untapped potential for drug discovery. few years by the discovery of the endogenous substances
which activate these receptors. The remaining orphan
Of these 800 genes, approximately 400 represent olfactory GPCRs are likely to contribute additional targets to these
receptors, and approximately 50 represent pseudogenes. and other disease areas.
Of the remaining 340 or so genes, approximately 200
represent known receptors and 140 represent unknown We are pleased to present this GPCR offering and look
or orphan receptors. Orphan receptors are receptors that forward to announcing new GPCRs to you routinely.
are known to be part of the GPCR family by virtue of their
molecular sequence but whose biology is unknown (in the
■ Storage Conditions
Storage at -80°C is recommended, repeated freeze-
thawing of this product is not recommended
Representative GPCR characterization data
7,500
GTPG 35S Bound (cpm)
5,000
2,500
0
-10 -8 -6 -4
Additional available information through the web site,
Carbachol (M) www.lonza.com/research, or Scientific Support at
Recombinant human M1 muscarinic receptor. 1-800-521-0390
A full listing of cell lines and membranes offered
Your Sales Representative contact information and quote request
General recommended assay conditions
Host cell
Protein concentration
GenBank accession number
EC50
Binding assay data lot specific
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Bioservices
Combining cell culture expertise with innovative bioassays and development capabilities
Bioassays are a critical part of the drug development Cytotoxicity and Apoptosis BioAssays
pipeline, from discovery through clinical trials to lot
Lonza Bioservices offers a number of bioassays to
release. The diverse skills of our Bioservices group make
address cell viability, cytotoxicity, apoptosis, and
us an excellent partner, whatever your bioassay needs,
proliferation in culture to determine test compound
whether for research and development purposes, or per
toxicity in the drug discovery process. These bioassays
cGMP or cGLP guidelines. We combine client-specific
take advantage of the power of ATP bioluminescent
project management with rapid and flexible turnaround
detection for exceptional sensitivity, reproducibility, and
time to meet your requirements. Bioservices specializes
rapid turnaround.
in the development, qualification, and validation of cell-
based bioassays. We apply the same world class expertise — Exceptionally sensitive with a detection limit of as few
Bioservices
used in development of more than a thousand cell culture as 10 cells per well
products. No one knows more about cells than we do. — Highly reproducible CV’s of <5%
— Effective tests for adherent and suspension
BioAssay Method Development
mammalian cells as well as bacteria and yeast
We have developed a wide range of bioassays to — Apoptosis bioassays correlate well with established
characterize biological and biopharmaceutical products. apoptosis assays, including TUNEL, propidium iodide,
Our capabilities include, but are not limited to: Annexin V, and caspase assays
Cell-Based BioAssays Biological Systems Analyzed
— Apoptosis — Adipogenesis
— Differentiation — Angiogenesis
— Proliferation — Hematopoiesis Typical cytotoxicity results for several drug compounds
using hepatocytes and hematopoietic progenitors
— Purity — Neurology
— Cytotoxicity — Osteogenesis Primary Human Hepatocytes
Luminescence (RLU)
12,000
— Necrosis
10,000
— Neutralization
8,000
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BioAssays FAQs
MycoAlert® Mycoplasma Detection Kit ViaLight® Cell Proliferation and Cytotoxicity Assays
Q. Does the kit recognize the whole spectrum of Q. How does the performance of the ViaLight®
mycoplasma? Assays compare with other assays routinely
used in cell proliferation/cytotoxicity assays?
A. The mycoplasma specific metabolism detected
by the MycoAlert® Kit is present in all members A. The combination of extreme sensitivity and wide
of the mollicutes family. 96% of all mycoplasma dynamic range of bioluminescent measurement
infections of cells grown in culture are caused allows the ViaLight® Assays to determine very
by just six species. The MycoAlert® Kit can subtle changes in the viability status of cultures
detect these species and many more; the list which reveal cytotoxicity missed by other
is ongoing. Please contact Scientific Support for
BioAssay FAQs
methods such as tetrazolium dyes, resazurin
further information. reagents and LDH assays.
Q. What type of sample do I need? Q. What is the difference between the ViaLight®
Can it be stored? HS and ViaLight® Plus Cell Proliferation and
A. The MycoAlert® Kit requires the use of cell free Cytotoxicity BioAssay Kits?
supernatant only! Ideally you should sample A. ViaLight® HS Assay can detect less than 10
your cell cultures and test immediately. If mammalian cells per microwell and measures
needed you can store the supernatant at room over a dynamic range of 5 decades. The ViaLight®
temperature for 8 hours, 2°C – 8°C for 1 week Plus Assay is designed to give enhanced signal
or 2 months at -20°C. (Thaw sample without stability and light output – signal half-life is in
the aid of artificial heat and equilibrate to room excess of 5 hours with greater reagent stability
temperature before use in assay). with the same sensitivity of the ViaLight® HS
Q. What about a positive control? Assay.
A. The MycoAlert® Assay Control Set (LT07-518) is
available separately and includes a lyophilized Continued on next page
positive control (1 ml) and assay buffer (2
ml) for reconstitution. The assay buffer also
serves as a negative control. The positive and
negative controls are designed for use with the
MycoAlert® Mycoplasma Detection Kit for the
detection of mycoplasma contaminations in cell
cultures. The Assay Control Set may be used to
ensure that the operator and all of the assay
reagents are performing properly.
The positive control is proprietary, but does NOT
contain mycoplasma. The positive or negative
control is simply substituted for a sample in the
standard assay protocol. The positive control
will give a ratio of >1 while the negative control
will give a ratio <1.
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BioAssays FAQs
Continued
Q. Can the ToxiLight® Assay be used for all cell Q. How does the ApoGlow® Assay differentiate
types? between apoptosis and necrosis?
A. Yes, the ToxiLight® Kit is intended for use with all A. Interpretation of the results is based on the
mammalian cell types, with both adherent and ratio of ATP to ADP. The interpretation of different
non-adherent cells. As adenylate kinase (AK) is ratios obtained, within each experimental
ubiquitous, the principle can also be applied to situation, may vary according to the cell types
bacterial, fungal and plant cells. and conditions used. However, in general terms,
if a test gives considerably lower ATP levels than
Q. How does the ToxiLight® Kit compare with
BioAssay FAQs
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BioWhittaker® Cell Culture
Chapter 5
BioWhittaker® Cell Culture
BioWhittaker®
Cell Culture
For further manufacturing or laboratory use. Not for use in diagnostic procedures.
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BioWhittaker® Classical Media
Classical Media
BioWhittaker®
Liquid Cell Culture Media
Chemically defined liquid media are used to provide Chemicals used to prepare liquid media products are
nutrients for cell culture growth in research, diagnostic, purchased according to the raw material qualifications
and manufacturing applications. In order to meet the from approved suppliers. Each lot must meet established
diverse needs of the markets we serve, the production component specifications before it is released by Quality
and quality control procedures for liquid media emphasize Assurance for use. We manufacture all liquid cell culture
control. Our goal is to provide customers with high quality media using Water for Injection (WFI) quality water, which
products that are consistent from batch to batch. With all has been prepared by ultrafiltration, reverse osmosis,
of the time that you take to optimize your systems, we want deionization, and distillation. Liquid media is sterile filtered
to provide you with products you can trust. through pharmaceutical-grade sterilizing filters. The for-
mulations used for standard liquid media products are
All BioWhittaker® Classical Media products produced are those recommended by the Tissue Culture Association.
manufactured in accordance with cGMP regulations. A continued on next page
Device Master Record (DMR) is prepared for every liquid
medium product. It defines the procedures for production
from receipt of raw materials to final product release,
the environmental and processing controls required,
and product specifications, including packaging and
labeling. Product manufacturing is consistent with the
requirements defined in the DMR, which ensures that
each lot of a product is consistent with all other lots of the
same product.
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BioWhittaker® Classical Media
Continued
Classical Media
BioWhittaker®
Quality Control
The combined result of all in-process monitoring and final
In order to maintain consistent quality in sterile cell culture
product testing is the further assurance that each lot has
media products, strict quality control of each production lot
been prepared not only according to approved written
is essential. Written procedures in accordance with current
procedures, but has passed test criteria, and will meet
Good Manufacturing Practices (cGMPs) provide quality
design specifications. Samples from each lot of standard
control from start to finish for each product produced.
product are retained and stored at label temperature in
Final product testing includes the following:
the event follow-up testing is required. Subsequent testing
may be in response to customer inquiries or for shelf life
USP Sterility – Tests are performed on representative samples studies.
using the membrane filtration procedure in accordance
with the US Pharmacopoeia or EP Pharmacopoeia. Culture Micronized Media (Powder)
media used are fluid thioglycollate medium (FTM) and
trypticase soy broth (TSB). All test media must pass Some tissue culturists prefer to prepare their own liquid
growth promotion testing before use. The test samples are medium from powder. Therefore, we are committed to
filtered and the filters are immersed in FTM and TSB. TSB offering you the same high quality powdered media as our
cultures are incubated at 22.5°C ± 2.5°C. FTM cultures are customers have come to expect with our liquid media. We
incubated at 32.5°C ± 2.5°C. The cultures are incubated for utilize unique manufacturing processes, which produce
14 days, during which they are periodically examined and the powder in a controlled environment, guaranteeing
sterility results are recorded. maximum stability and minimum degradation due to
chemical oxidation. Standard package sizes are 10 L
Chemistries (pH and osmolality) – Tests are performed and 50 L.
on representative samples from each lot using routinely
calibrated equipment. Osmolality is determined by means Quality Control Testing
of the highly-repeatable freezing point method. All powdered media are subject to rigorous quality control
testing. These tests include: pH, osmolality, cell growth,
Cell Growth Promotion – Media and supplements are endotoxin, moisture content, and a sieve test.
tested for their growth promoting properties using
primary, diploid, and heteroploid cell lines unless other Shelf Life
cells are appropriate based on the product use. Results Powdered media are generally very stable at refrigerated
are obtained using the Lowry protein method or manual temperatures. Shelf life is two years on most powdered
cell count to assess cell replication. Visual screening products.
of cultures is also done to ensure characteristic cell
For information about how to prepare powdered media, please refer to the
morphology and lack of toxicity. Technical Information section at the end of this chapter, page 303
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BioWhittaker® Classical Media
Continued
Classical Media
BioWhittaker®
Ordering Information Ordering Information
Basal Medium Eagle (BME) Cat. No. Size Price
A minimal medium suitable for a variety of cell types. It is a historical precursor
to Minimum Essential Medium (MEM).
Dulbecco’s Modified Eagle’s Medium
with 4.5 g/L glucose, without L-glutamine
Storage Conditions: 2°C – 8°C
Cat. No. Size Price
12-614F 500 ml
Cryoprotective Medium 12-604F/12 12 x 500 ml
without L-glutamine, with 15% DMSO (Use 1:1 with growth medium)
12-614Q 1L
Storage Conditions: 15°C – 30°C
12-604Q/12 12 x 1 L
12-132A 100 ml
Dulbecco’s Modified Eagle’s Medium, Powder
See also ProFreeze™-CDM, Non-Animal Origin, Chemically Defined Freeze Medium, with 4.5 g/L glucose, without L-glutamine or sodium pyruvate
Cat. No. 12-769E, page269. Storage Conditions: 2°C – 8°C
See page 300 for a basic procedure for cryopreservation of cells.
15-614D 1 × 10 L
Basal Medium Eagle with Earle’s BSS
without L-glutamine Dulbecco’s Modified Eagle’s Medium
Storage Conditions: 15°C – 30°C with 4.5 g/L glucose, with L-glutamine
Storage Conditions: 2°C – 8°C
12-105F 500 ml
12-604F 500 ml
12-604F/12 12 x 500 ml
Dulbecco’s Modified Eagle’s Medium (DMEM) 12-604Q 1L
12-604Q/12 12 x 1 L
DMEM is used in a wide range of mammalian cell culture applications. The
high glucose version is well suited to high density suspension culture. The
low glucose formula is used for adherent dependent cells. Dulbecco’s Modified Eagle’s Medium
with 4.5 g /L glucose, with UltraGlutamine I
Storage Conditions: 2°C – 8°C
BE12-604F/U1 500 ml
15-604D 1 × 10 L
Without sodium pyruvate
Hybridoma screened
With UltraGlutamine I
With 1.0 g/L glucose
With 4.5 g/L glucose
Without L-glutamine
BE15-604F 1 × 50 L
Without phenol red
With L-glutamine
15-604D 1 ✕ 10 L
■ ■ ■ ■
BE15-604F 1 ✕ 50 L
12-914F 500 ml ■ ■ ■
12-917F 500 ml ■ ■ ■
12-707F 500 ml ■ ■
12-708F 500 ml ■ ■ ■
12-709F 500 ml ■ ■ ■
12-733F 500 ml
■ ■ ■
12-733Q 1L
12-741F 500 ml ■ ■ ■
With UltraGlutamine I
With L-glutamine
12-707F 500 ml
Without HEPES
With HEPES
Dulbecco’s Modified Eagle’s Medium
with 1.0 g/L glucose and 25 mM HEPES Buffer, without L-glutamine
Storage Conditions: 2°C – 8°C Cat. No. Size
12-719F 500 ml
■ ■
12-708F 500 ml 12-719Q 1L
BE04-687Q 1L ■ ■
Dulbecco’s Modified Eagle’s Medium
with 4.5 g/L glucose and 25 mM HEPES Buffer, without L-glutamine BE04-687F/U1 500 ml ■ ■
Storage Conditions: 2°C – 8°C
Please see the Product Formulations section for exact compositions.
12-709F 500 ml
Ordering Information
Dulbecco’s Modified Eagle’s Medium
with 4.5 g/L glucose, without L-glutamine or sodium pyruvate Cat. No. Size Price
Storage Conditions: 2°C – 8°C
DMEM:F-12, 1:1 Mixture
12-733F 500 ml with 3.151 g/L glucose, L-glutamine, and 15 mM HEPES Buffer
Storage Conditions: 2°C – 8°C
12-733Q 1L
12-719F 500 ml
Dulbecco’s Modified Eagle’s Medium
with 4.5 g/L glucose, with L-glutamine, without sodium pyruvate 12-719Q 1L
Storage Conditions: 2°C – 8°C
DMEM:F-12 1:1 Mixture
12-741F 500 ml with 3.151 g/L glucose, L-glutamine, without HEPES
Storage Conditions: 2°C – 8°C
BE04-687Q 1L
BE04-687F/U1 500 ml
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BioWhittaker® Classical Media
Continued
Classical Media
BioWhittaker®
Glasgow Minimum Essential Medium (GMEM) Iscove’s Modified Dulbecco’s Medium (IMDM)
Designed to support BHK-21 cells. IMDM is suitable for fast growing cells. All formulas contain HEPES for added
buffering.
Ordering Information
Cat. No. Size Price
Hybridoma screened
Without L-glutamine
Glasgow Minimum Essential Medium
With L-glutamine
(G-MEM, BHK-21)
With HEPES
Storage Conditions: 2°C – 8°C
12-726F 500 ml
Cat. No. Size Price ■ ■
12-726Q 1L
Grace’s Insect Medium 12-915F 500 ml ■ ■ ■
without lactalbumin hydrolysate, yeastolate, gentamicin or FBS
Storage Conditions: 2°C – 8°C Please see the Product Formulations section for exact compositions.
12-618F 500 ml
BE02-014F 500 ml
12-615F 500 ml
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BioWhittaker® Classical Media
Continued
Classical Media
BioWhittaker®
12-669E 100 ml
With UltraGlutamine I
Without L-glutamine
With nucleosides
With L-glutamine
Without calcium
With HEPES
With HBSS
With NEAA
With EBSS
Powder
Cat. No. Size
12-169F 500 ml ■ ■ ■
BE02-002F 500 ml ■ ■ ■ ■
12-611F 500 ml
■ ■
12-611Q 1L
15-611D 1 ✕ 10 L ■ ■ ■ ■
12-125F 500 ml
■ ■
12-125Q 1L
12-662F 500 ml
■ ■ ■ ■
12-662Q 1L
12-136F 500 ml
■ ■ ■
12-136Q 1L
12-736E 100 ml
■ ■ ■ ■ ■
12-736F 500 ml
12-684F (10X) 500 ml ■ ■ ■
06-174G 450 ml ■ ■ ■ ■
BE02-020F 500 ml ■ ■ ■
12-127F 500 ml ■ ■
12-137F 500 ml ■ ■ ■
04-719Q (Joklik’s) 1L ■ ■
Classical Media
BioWhittaker®
Ordering Information Ordering Information
MEM Alpha Eagle with Earle's BSS MEM Eagle with Earle’s BSS
without L-glutamine, deoxyribonucleosides or ribonucleosides Cell culture maintenance medium 12-136 with NEAA, L-glutamine, 25
Storage Conditions: 2°C – 8°C mM HEPES buffer, 10 µg/ml gentamicin, 50 units/ml penicillin, 50 µg/ml
streptomycin, 2.5 µg/ml amphotericin B, and 2.0% heat-inactivated FBS
12-169F 500 ml Storage Conditions: 2°C – 8°C
BE02-002F 500 ml
MEM Eagle with Earle’s BSS (10X)
without NaHCO3 or L-glutamine
Storage Conditions: 15°C – 30°C
MEM Eagle with Earle’s BSS
with L-glutamine 12-684F 500 ml
Storage Conditions: 2°C – 8°C
12-611F 500 ml
MEM Eagle with Earle’s BSS (2X)
without L-glutamine or phenol red (virus plaquing medium)
12-611F/12 12 x 500 ml Storage Conditions: 15°C – 30°C
12-611Q 1L 12-668E 100 ml
12-611Q/12 12 x 1 L BE12-668F 500 ml
MEM Eagle with Earle’s BSS (Powder) MEM Eagle with Earle’s BSS
with L-glutamine with non-essential amino acids and L-glutamine, without calcium
Storage Conditions: 2°C – 8°C Storage Conditions: 2°C – 8°C
15-611D 1 × 10 L 06-174G 450 ml
MEM Eagle with Earle’s BSS MEM Eagle with Earle’s BSS D-Val Medium
without L-glutamine without L-glutamine, with D-valine substituted for L-valine, use dialyzed serum
Storage Conditions: 15°C – 30°C (14-810F) to supplement
Storage Conditions: 15°C – 30°C
12-125F 500 ml
12-125F/12 12 x 500 ml BE02-020F 500 ml
12-125Q 1L
MEM Eagle with Hanks’ BSS
12-125Q/12 12 x 1 L without L-glutamine
Storage Conditions: 15°C – 30°C
MEM Eagle with Earle’s BSS
with non-essential amino acids and sodium pyruvate, without L-glutamine 12-127F 500 ml
Storage Conditions: 2°C – 8°C
MEM Eagle with Hanks’ BSS
12-662F 500 ml with 25 mM HEPES Buffer, without L-glutamine
12-662Q 1L Storage Conditions: 15°C – 30°C
12-137F 500 ml
MEM Eagle with Earle’s BSS
with 25 mM HEPES Buffer, without L-glutamine
Storage Conditions: 15°C – 30°C MEM Eagle Joklik’s Formulation
for suspension cultures, with L-glutamine
12-136F 500 ml Storage Conditions: 2°C – 8°C
12-136Q 1L 04-719Q 1L
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BioWhittaker® Classical Media
Continued
Classical Media
BioWhittaker®
12-923E 100 ml
With L-glutamine
With HEPES
With HBSS
With EBSS
Richter’s CM Medium
Cat. No. Size Also known as I-MEM – Improved MEM with zinc.
12-109F 500 ml ■ ■ ■ Cat. No. Size Price
12-117F 500 ml
12-117Q 1L
■ ■ ■ Richter’s CM Medium
with L-glutamine
12-118F 500 ml ■ ■ ■ ■ Storage Conditions: 2°C – 8°C
12-119F 500 ml ■ ■ 04-648F 500 ml
Please see the Product Formulations section for exact compositions. RPMI 1640 Medium
RPMI is a general purpose media with a broad range of applications
Ordering Information for mammalian cells, especially hematopoietic cells. RPMI with MOPS
(04-525F) is for certain mycological assays.
With UltraGlutamine I
Without L-glutamine
Without D-glucose
With MOPS buffer
With L-glutamine
Medium 199 with Earle’s BSS
with L-glutamine, 25 mM HEPES Buffer, and 2.2 g/L NaHCO3
With HEPES
Storage Conditions: 2°C – 8°C
Powder
12-117F 500 ml Cat. No. Size
08-012B 10 L ■
Medium 199 with Earle’s BSS
with L-glutamine and 2.2 g/L NaHCO3 BE12-115E 100 ml
Storage Conditions: 2°C – 8°C 12-115F 500 ml ■ ■
12-115Q 1L
12-119F 500 ml
BE12-115F/U1 500 ml ■ ■
04-525F 500 ml ■ ■ ■
09-774E 100 ml
■ ■ ■
09-774F 500 ml
12-918F 500 ml ■ ■
BE12-752F 500 ml ■ ■
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BioWhittaker® Classical Media
Continued
Classical Media
BioWhittaker®
Ordering Information Ordering Information
Williams' Medium E
without L-glutamine or phenol red
Storage Conditions: 2°C – 8°C
BE02-019F 500 ml
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Notes
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Specialty Media
Specialty Media
Serum-free media and reagents have a wide range of Serum-free media formulations must satisfy a number
applications, including production of monoclonal antibodies, of nutritional and physical requirements of cells that
viral antigens, and recombinant proteins using a variety of are normally addressed by the presence of serum.
mammalian and invertebrate cell lines. There are numerous Serum proteins such as albumin, fibronectin, and fetuin
advantages associated with the use of serum-free media serve a variety of functions that include adsorbing toxic
formulations: compounds, providing protection against shear forces
■ Increased definition in bioreactors, creating a matrix for cellular attachment
to surfaces, and acting as a carrier for lipids and other
■ Increased lot-to-lot consistency growth factors.
■ Simplified purification and downstream processing
For information about adaptation of cell cultures to serum-
■ Better control over the physiological condition of cultures
free media, please refer to the Technical Information
■ Ability to optimize formulations for specific cell types section, page 298.
Specialty Media
Introduction 261 ProVero™ 1 Serum-free Medium 270
UltraCULTURE™ Serum-free Medium 262 ProPer™ 1 Chemically Defined, Serum-free Medium 271
PC-1™ Chemically Defined, Serum-free Medium 263 Human Tubal Fluid 272
UltraMEM™ Reduced Serum Media 264 Lymphochrome™ Medium 272
X-VIVO™ Chemically Defined, Serum-free Amniochrome® II Modified Medium 273
Hematopoietic Cell Media 265 Amniochrome® Plus Medium 273
UltraCHO™ Serum-free CHO Cell Media 266 Amniochrome® Pro Medium 273
ProCHO™ Protein-free CHO Media 267 HL-1™ Chemically Defined, Serum-free Media 274
PowerCHO® Chemically Defined, UltraDOMA™ Serum-free Hybridoma Medium 276
Serum-free CHO Media 268 ProDoma™ Serum-free Hydridoma Media 277
ProFreeze™ -CDM, NAO, Chemically Defined UltraDOMA-PF™ Protein-free Hydridoma Media 277
Freeze Medium (2X) 269 Insect-XPRESS™ Protein-free Insect Cell Medium 278
UltraMDCK™ Chemically Defined, ProNS0™ Chemically Defined, Protein-free Media 279
Serum-free Renal Cell Medium 269
Pro293™ Chemically Defined, Serum-free Media 270
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UltraCULTURE™ Serum-free Medium
General purpose serum-free medium
(Cat. No. 12-132A) to cryopreserve cells in a serum-free HuL-1,2 Human Liver (normal)
environment. UltraCULTURE™ Medium does not contain HuK-1 Human Kidney (normal)
L-glutamine; add 5 ml of 200 mM L-glutamine solution HuS-1AT Human Skin
(Cat. Nos. 17-605 or 17-905) prior to use. HEC Human Embryonic cancer
HL-60 Human Acute promyelocytic leukemia
The formulation for UltraCULTURE™ Medium has been Raji Human Burkitt’s lymphoma
submitted to the FDA as a Master File. Permission to cross- EB-3 Human Burkitt’s lymphoma
reference the Master File may be obtained by contacting K-562 Human Chronic myelocytic leukemia
the Regulatory Affairs Department. HNK Human Neonatal kidney (primary)
HTC29 Human Colon cancer
■ Applications
TT Human Medullary thyroid tumor
– Cultivation of adherent and non-adherent mammalian MB231 Human Breast carcinoma
cells U138 Human Glioma
– Generation of viral particles for vaccine production FM3A Mouse Breast cancer
NS-1 Mouse Myeloma
■ Performance and Quality Tests
L Mouse Subcutaneous
– Turbidity may develop in UltraCULTURE™ Medium; P388D1 Mouse Macrophage-like
experiments have determined that the turbidity will
P815 Mouse Mast cell tumor
not alter the performance of the product
T3 Mouse Pituitary
■ Storage Conditions B82 Mouse L cells - connective tissue
2°C – 8°C RPL-1 Rat Peritoneum
RSP-2 Rat Spleen
RLG-1 Rat Lung
Lym-1 Rat Lymph node
RCR-1 Rat Brain
235-1, MMQ Rat Pituitary
GC, GH3 Rat Pituitary
CA77 Rat Medulary thyroid tumor
Rat-1 Rat Fibroblast
JTC-12 Monkey Kidney
COS1, COS7 African green SV40 transformed kidney
monkey
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined, Freeze Medium (2X) 269
262
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PC-1™ Chemically Defined, Serum-free Medium
General purpose serum-free medium
Serum-free Medium
using defined components for optimal cell growth, while
maintaining the lowest possible protein content.
■ Applications
PC-1™
– Cultivation of primary and anchorage-dependent cells
■ Storage Conditions
2°C – 8°C
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined, Freeze Medium (2X) 269
263
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UltraMEM™ Reduced Serum Media
General purpose serum-free media
12-743F 500 ml
Further reduction in serum concentration (<2%) can be
UltraMEM™
ITS/ITES should not be refrozen. It can be kept refrigerated (2°C – 8°C) for
up to a month.
1. ITS/ITES means either one supplement or the other.
2. In the following cell lines Fetal Bovine Serum can be replaced with Serum
Supreme (Cat. No. 14-492F): CRFK, MDCK, 3T3, CHO-K1, PK-15, BGMK
and MK2.
Related Products
ITES 283
ITS 283
264
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X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Media
Serum-free media for hematopoietic cells
X-VIVO™ Media
with L-glutamine, without gentamicin or phenol red
and murine lymphocytes. The formulations are complete
and contain pharmaceutical grade human albumin, 04-743Q 1L
recombinant human insulin, and pasteurized human
transferrin. X-VIVO™ 15 Chemically Defined, Serum-free
Hematopoietic Cell Medium
with L-glutamine, gentamicin, and phenol red
All X-VIVO™ Media products are manufactured under current
GMPs and are listed with the FDA in a product Master File. BE04-418F 500 ml
15-724F 1 × 50 L
UltraCHO™ Media are manufactured under FDA current
GMPs and the formulation has been submitted to the FDA as UltraCHO™ Supplement
non-sterile, for use with UltraCHO™ Powdered medium
a Master File. Permission to cross-reference the file may be
obtained from the Regulatory Affairs Department. 17-811A 100 ml
■ Applications
– Growth of CHO cells
– Recombinant protein production
■ Storage Conditions ■ Partial list of cell types cultivated with UltraCHO™
Liquid medium: 2°C – 8°C Medium
Powdered medium: 2°C – 8°C – Transfected and non-transfected CHO cell lines
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ProCHO™ Protein-free CHO Media
Non-animal origin CHO expression media
Protein-free Media
— ProCHO™ 4 Medium – For concurrent transition of with 0.1% Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or
L-glutamine
adherent CHO cells to serum-free and suspension
ProCHO™
culture; supports faster doubling times 04-919Q 1L
— ProCHO™ 5 Medium – For CHO cells already growing in ProCHO™ 4 Protein-free CHO Medium, Powder
suspension; supports increased protein production includes base powder and required supplements
with 0.1% Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or
— ProCHO™ AT Medium – For adherent culture of CHO L-glutamine
cells BE15-029D 1 × 10 L
BE15-029F 1 × 50 L
ProMEDIA SELECT® CHO Media Optimization Kit
Kit contains 16 distinct formulas that contain no materials ProCHO™ 5 Protein-free CHO Medium
with 0.1% Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or
of animal origin. It can be used to develop the most L-glutamine
optimized formulas for protein expression in CHO cells.
Media formulations are scalable to production volumes. 12-766Q 1L
BE02-016Q 1L
BE18-002Q Kit, 16 x 1 L
Related Products
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
ProHT™ Supplement (100X) 283
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PowerCHO® Chemically Defined, Serum-free CHO Media
Non-animal origin CHO expression media
00189269 1L
2000
1500
1000
500
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
268
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ProFreeze™- CDM, NAO, Chemically Defined Freeze Medium (2X)
Non-animal origin freezing medium
Renal Media
ProFreeze™ Medium requires the addition of 15% reagent
or spectrophotometric grade dimethylsulfoxide (DMSO) at
time of use. One bottle will make 117.6 ml of complete 2X
concentrated freezing medium after the addition of 17.6
ml DMSO. For best results, keep on ice during use.
■ Storage Conditions
2°C – 8°C
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Pro293™ Chemically Defined, Serum-free Media
Non-animal origin renal cell expression media
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
Related Products
ProFreeze™- CDM, NAO, Chemically-Defined Freeze Medium (2X) 269
270
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ProPer™ 1 Chemically Defined, Serum-free Medium
Non-animal origin medium for human embryonic retinoblast cells
Serum-free Medium
– Recombinant protein and virus production
– Growth of human embryonic retinoblast cells (PER.C6®
ProPer™ 1
and related cell lines) in suspension
■ Storage Conditions
2°C – 8°C
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
271
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IVF Culture Media
Mouse embryo tested
BE02-036F 500 ml
IVF Culture
Lymphochrome™ Medium
Karyotyping medium
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Cytogenetics Media
Specialty Media
BE12-756EZM 100 ml
laboratory for the cultivation of amniotic fluid cells for
Cytogenetics
BE12-756FCM 500 ml
cytogenetic analysis.
CE marked according to IVD Directive 98/79/EC
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
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HL-1™ Chemically Defined, Serum-free Media
Continued
Partial list of cell cultures cultivated with HL-1™ Chemically Defined, Serum-free Medium
Hybridoma Media
HL-1™ Serum-free
NIH ZR-75 Human Breast carcinoma HB45 Mouse Sp2/0-Ag14
COLO 302 HSR Human Colon carcinoma HB56 Mouse NS-1
J82 Human Bladder carcinoma HB59 Mouse NS-1
SW 1738 Human Bladder carcinoma HB60 Mouse P3X63Ag 8.653
SW780 Human Bladder carcinoma 53-7.313 Mouse NS-1
CCL 119 Human Lymphoid MI/9.3.4HL-2 Mouse NS-1
CCL 213 Human Burkitt lymphoma MI/70.15.1 Mouse NS-1
C91/PL Human T lymphoma ARB Mouse Hybridoma
Undesignated Human Astrocytoma P3U Mouse P3X63Ag 8.653
Undesignated Human Hepatoma BCS12 Mouse P3X63Ag 8.653
MOLT-3 Human Acute lymphoblastic leukemia BCS 2002 Mouse P3X63Ag 8.653
MOLT-4 Human Acute lymphoblastic leukemia undesignated Mouse NS-1
NAMALWA Human Burkitt lymphoma undesignated Mouse P3X63Ag 8.653
THP-1 Human Monocytic leukemia TIB 175 Rat/mouse S194
BB88 Mouse Erythroid (leukemia) TIB 104 Rat/mouse NS-1
P815 Mouse Macrophage TIB 105 Rat/mouse NS-1
P388D1 Mouse Macrophage TIB 109 Rat/mouse NS-1
WeHi3 Mouse Monocyte TIB 128 Rat/mouse NS-1
JLS-V5 Mouse Spleen cell TIB 166 Rat/mouse NS-1
70Z-3 Mouse Pre-B lymphoma TIB 168 Rat/mouse NS-1
70Z/3.12 Mouse B lymphoma RS Rat/mouse P3X63Ag 8.653
S49 and variants Mouse T lymphoma
RAW309F1.1 Mouse T lymphoma
WeHi7 Mouse T lymphoma
Primary cells
I-10 Mouse Leydig-tumor
EL4 Mouse T lymphoma Cell Type
RL1 Mouse T lymphoma Human peripheral blood mononuclear cells
BW5147.3 Mouse T lymphoma Mink lymphocytes
LBRM-33 Mouse T lymphoma Human fetal adrenal
Friend leukemia Mouse Leukemia Human blood monocytes
C57BL6 Mouse Embryo (C57 X DBA) Human peripheral blood T lymphocytes
L5178Y Mouse Lymphoma (DBA/2)
VERO African green monkey Fibroblast
MDCK Dog Madin Darby canine kidney
CHO K1 Hamster Chinese hamster ovary
(epithelial-like)
GCL2 Hamster/mouse B lymphoma X Normal B
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UltraDOMA™ Serum-free Hybridoma Medium
Hybridoma medium
bovine albumin. The total protein concentration is 30 µg/ 12-723B 500 ml (glass bottle)
ml. UltraDOMA™ Medium does not contain L-glutamine. BE12-723F 500 ml (plastic bottle)
UltraDOMA™ Serum-free
can be maintained in the medium indefinitely and can be Partial list of cell types cultivated with UltraDOMA™ Medium
cryopreserved in UltraDOMA™ Medium supplemented with
Cell Type
Cryoprotective Freezing Medium (Cat. No. 12-132A).
Murine hybridomas
■ Applications NS-1 derived myelomas
– Hybridoma cell growth SP-2 derived myelomas
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
UltraGlutamine 284
276
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ProDoma™ Serum-free Hybridoma Media
Non-animal origin hybridoma media
Hybridoma Media
with 0.1% Pluronic® F-68, without phenol red, or L-glutamine
– Monoclonal antibody production
BE02-032Q 1L
■ Storage Conditions
2°C – 8°C
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Insect-XPRESS™ Protein-free Insect Cell Medium
Insect cell expression medium
Cell densities in excess of 8.3 × 106 cells/ml can be achieved Insect-XPRESS™ Protein-free Insect Cell Medium
with suspension cultures of Sf9 cells using Insect-XPRESS™ with L-glutamine
Medium and an excess of oxygen. This formulation can 12-730F 500 ml
also be used for stationary monolayer cultures and 12-730Q 1L
shake-flask cultures. Insect-XPRESS™ Medium contains
Protein-free Medium
Related Products
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
278
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ProNS0™ Chemically Defined, Protein-free Media
Non-animal origin NS0 expression media
The NS0 cell line is widely used for recombinant mammalian Ordering Information
protein expression. Some of the reasons to select this mouse
Cat. No. Size Price
myeloma platform are:
— Forms stably producing hybrid cells with high levels of ProNS0™ 1 Chemically Defined, Protein-free Medium
with HEPES and Pluronic®, without L-glutamine, phenol red, or cholesterol
protein production
12-773Q 1L
— Lacks ability to secrete endogenous antibody or
antibody fragments
ProNS0™ 2 Chemically Defined, Protein-free Medium
— Resists aggregation clumping in suspension culture with HEPES and Pluronic®, without L-glutamine, phenol red, or cholesterol
Protein-free Media
— Lacks adverse protease activity on recombinant 12-774Q 1L
ProNS0 ™
protein product (in many cases)
ProNS0™ Lipid Chemically Defined Supplement
ProNS0™ 1 and 2 CD Media, together with ProNS0™ Lipid with cholesterol, suggested use is 5 ml/L.
CD Supplement, are designed to meet a growing demand
12-775J 5 ml
for optimized NS0 formulations.
— Two optimized formulas are available to cover the
broadest range of NS0 nutritional needs Protein Production – ProNS0™ Media
IgG (mg/ml)
0.2 Day 6 mg/ml
— Further maximize protein production by titration with
ProNS0™ Lipid CD Supplement 0.15
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
279
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Notes
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Cell Culture Reagents
BioWhittaker® Cell Culture Reagents include a number of Chemicals used to prepare cell culture reagents are
different products, such as amino acids, antibiotics, and purchased according to the raw material qualifications
buffers, all of which are used routinely in research, and from approved suppliers. Each lot must meet established
manufacturing applications involving cell culture. component specifications before it is released by Quality
Assurance for use. We manufacture all liquid cell culture
Products are manufactured under the same cGMP conditions reagents using Water for Injection (WFI) quality water,
notE: All balanced salt solutions contain phenol red and Hanks’ BSS (1X)
naHCo3, unless otherwise indicated.
04-315Q 1L ■ ■
08-003A 4 L (bag) ■ ■
Ordering Information
10-508F 500 ml
10-508Q 1L
10-543F 500 ml
10-543Q 1L
10-527F 500 ml
10-527F/12 12 × 500 ml
10-547F 500 ml
04-315Q 1L
08-003A 4L
the 4 L size is provided in a flexible package with one female luer connector,
one blood spike receptacle, and one male MPC connector with cap.
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Reagents
ITS (500X) MEM Non-Essential Amino Acid Solution (10 mM, 100X)
Supplement of insulin, transferrin, and selenium 100X stock solution contains a 10 mM concentration of each non-essential
Storage Conditions: -10°C – -20°C amino acid
Storage Conditions: 2°C – 8°C
17-838Z 5 ml
13-114E 100 ml
ITES (500X)
Supplement of insulin, transferrin, ethanolamine, and selenium MEM Eagle Vitamin Mixture (100X)
Storage Conditions: -10°C – -20°C Storage Conditions: -20°C
17-839Z 5 ml 13-607C 50 ml
Reagents
17-605E 100 ml
17-605F 500 ml Sodium Bicarbonate (Powder)
Storage Conditions: 15°C – 30°C
L-glutamine (200 mM) 15-613I 500 g
Supplied at 29.3 mg/ml in 0.85% naCl and hybridoma screened
Storage Conditions: -10°C – -20°C
Sodium Pyruvate Solution (100 mM)
17-905C 50 ml 11.1 g/L
Storage Conditions: 2°C – 8°C
17-942E 100 ml
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Reagents
Continued
BE17-605E/U1 100 ml
BE04-684E 100 ml
284
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Antibiotics and Antimycotics
and Antimycotics
Penicillin-Streptomycin Mixture
Storage Conditions: 15°C – 30°C contains 25,000 units potassium penicillin and 25,000 µg streptomycin
Antibiotics
sulfate per ml in 0.85% saline
17-519Z 1 × 10 ml Storage Conditions: -10°C – -20°C
17-519L 10 × 10 ml
17-719R 25 × 4.5 ml
Gentamicin Sulfate
10 mg/ml Penicillin-Streptomycin-Amphotericin B Mixture
Storage Conditions: 15°C – 30°C contains 10,000 units potassium penicillin, 10,000 µg streptomycin sulfate
and 25 µg Amphotericin B per ml in 0.85% saline
BE02-012E 100 ml Storage Conditions: -10°C – -20°C
17-745H 20 ml
17-745E 100 ml
Penicillin-Streptomycin-L-glutamine Mixture
contains 25,000 units potassium penicillin, 25,000 µg streptomycin sulfate,
and 29.2 mg L-glutamine per ml
Storage Conditions: -10°C – -20°C
Penicillin-Streptomycin Mixtures
17-718R 25 × 4.5 ml
25 µg/ml Amphotericin B
20,000 µg streptomycin
25,000 µg streptomycin
10,000 µg streptomycin
With L-glutamine
17-602E 100 ml
■
17-602F 500 ml
09-757F 500 ml ■
17-719R 25 ✕ 4.5 ml ■
17-745H 20 ml
■ ■
17-745E 100 ml
17-718R 25 ✕ 4.5 ml ■ ■
17-512F 500 ml
Dulbecco’s Phosphate Buffered Saline 17-512F/12 12 x 500 ml
17-512F/24 24 x 500 ml
17-512Q 1L
Without calcium or magnesium
With calcium and magnesium
17-512Q/12 12 x 1 L
With sodium pyruvate
of 17-512
Storage Conditions: 15°C – 30°C
Powder
Buffered Salines
1 × 10 L
17-513F 500 ml
17-513Q 1L
■ ■ 15-456F 1 × 50 L
17-512F 500 ml BE15-512D 1 × 10 L
■ ■
17-512Q 1L
BE15-512F 1 × 50 L
15-456D 1 ✕ 10 L
15-456F 1 ✕ 50 L
BE15-512D 1 ✕ 10 L
■ ■ ■ Dulbecco’s Phosphate Buffered Saline (1X)
BE15-512F 1 ✕ 50 L 0.0095 M (Po4) with calcium, magnesium, 1 g/L glucose, and 36 mg/L
sodium pyruvate. Used in animal embryo transfer procedures.
04-479Q 1L ■ ■ ■ ■ Storage Conditions: 15°C – 30°C
17-515F (10X) 500 ml
■ ■ 04-479Q 1L
17-515Q (10X) 1L
Please see the Reagents Formulations section for exact compositions. Dulbecco’s Phosphate Buffered Saline (10X)
0.095 M (Po4) without calcium or magnesium
Storage Conditions: 15°C – 30°C
17-515F 500 ml
17-515Q 1L
HEPES Buffer (1 M)
contains 238.3 g/L in normal saline
Storage Conditions: 15°C – 30°C
17-737E 100 ml
17-737F 500 ml
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Buffers and Buffered Salines
Continued
With HEPES
PBS EDTA
used as buffer in procedures of generating human dendritic cells from Cat. No. Size
monocytes, pH 7.5 solution Phosphate Buffered Saline
Storage Conditions: 15°C – 30°C
17-516F 500 ml
■ ■
BE02-017F 500 ml 17-516Q 1L
04-409R (Carboy) 15 L ■ ■
Buffered Salines
17-516Q/12 12 x 1 L
Buffers and
Phosphate Buffered Saline (1X)
0.0067 M (Po4) without calcium or magnesium
Storage Conditions: 15°C – 30°C
04-409R 15 L
Carboy with dispensing rigging, one male luer fitting and 5 feet of tubing.
17-517Q 1L
TL HEPES Solution
Storage Conditions: 2°C – 8°C
04-616F 500 ml
UltraSaline A
HEPES buffered saline without phenol red; enhances trypsin action when
used to rinse monolayer before subculture
Storage Conditions: 2°C – 8°C
12-747F 500 ml
12-624E 100 ml
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Notes
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Animal and Human Sera
BioWhittaker® Brand Sera and Media products from Lonza All sera, including bovine, equine and human, are aseptically
have been the leaders in quality for over 50 years. Our collected and must meet established component specifi-
strict adherence to current Good Manufacturing Practices cations before they are accepted and released by Quality
(cGMP) results in the highest quality serum products Assurance for use in further manufacturing. Daily
available. From raw material collection to final product collection records indicate the day, month and year the
release, in-process quality assurance tests are designed raw blood was collected and provide traceability to the
to ensure that the products we supply meet our own individual pool, which provides traceability back to the
high quality standards as well as our clients’ stringent USDA establishment number and abattoir or donor herd
requirements. from which the raw material originated.
All US-origin animal sera are collected from abattoirs Prior to filtration, the serum is dispensed into a batch
inpected by the USDA and located within the contiguous tank and mixed to assure bottle-to-bottle homogeneity.
48 states. USDA approved source fetal bovine serum (Cat. All fetal bovine serum is sterile filtered through three
No. 14-502) is USDA certified and approved for importation 0.1-micron filters.
continued on next page
into the United States.
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Animal and Human Sera
Continued
Serum Donor
Animal and Human Sera
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Animal and Human Sera
Continued
Cell Growth Promotion – All serum lots of animal origin Chlamydia Functional Testing—Fetal bovine serum (Cat.
are tested for their growth promoting properties using No. 14-902E) labeled for chlamydia isolation is tested in
primary, diploid, and heteroploid cell lines unless other a controlled functional test. Dilutions of LGV1 or LGV2 are
cells are appropriate based on the product’s use. Results isolated in McCoy or L929 cells. Cultures are stained with
are obtained using the Lowry protein method to assess Jones Iodine for inclusion counts which must be >75% of
cell replication. Visual screening of cultures is also done to the control.
ensure characteristic cell morphology and lack of toxicity.
Origin and Types of Sera
Chemistry – pH, osmolality, total protein and hemoglobin Fetal Bovine Serum, Premium, US Origin
determinations are performed for each lot of serum. All whole fetal bovine serum is aseptically collected using a
Osmolality is determined by means of the highly repeatable cardiac puncture technique from USDA inspected abattoirs
freezing point method. Total protein is determined by the located within the 48 contiguous United States. Each lot
Biuret method. An extensive panel of chemical tests, is completely traceable back to the USDA establishment
including a biochemical profile, is run on each lot of animal number from which the raw material was obtained. Donor
serum. The biochemical profile includes: animals receive ante- and post-mortem inspection by an
Albumin Gamma Globulin SGOT on-site USDA veterinary inspector and appear to be free of
Alkaline Phosphatase Glucose SGPT infectious diseases.
Blood Urea Nitrogen Glutamyl Transpeptidase Sodium
Calcium % Gamma Globulin Total Bilirubin Fetal Bovine Serum, USDA Approved Source
Chloride Iron Total Protein All whole fetal bovine serum is aseptically collected using a
Cholesterol Lactic Dehydrogenase Triglycerides cardiac puncture technique from USDA approved sources.
Creatinine Phosphorus Uric Acid Each lot produced is safety tested for exotic viruses and
Free Fatty Acids Potassium
approved for importation into the United States by the
USDA. Donor animals receive ante- and post-mortem
Serum Identity – Samples are tested for characteristic
inspection by an on-site USDA veterinary inspector and
electrophoretic separation of proteins that appear
appear to be free of infectious diseases.
as visible bands or precipitate when the appropriate
antiserum is allowed to react with them. This technique Fetal Bovine Serum, Premium, US Origin, Irradiated
allows verification of serum speciation. Irradiated fetal bovine serum is produced in the same
manner and from the same high quality US origin fetal
bovine serum as our premium FBS and irradiated to reduce
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Animal and Human Sera
Continued
FBS, US Origin, Irradiated (14-471) is routinely tested for Heat Inactivation of Serum
the presence of bovine viruses by full compliance 9CFR We recommend the following protocol for heat inactivation:
test procedures. These results include test results for the 1. Place the serum at room temperature for 3 – 4 hours
following bovine viruses: Bovine Viral Diarrhea (BVD), IBR before thawing the serum completely in a 37°C water
(CPE), PI3 (hemadsorption), Bovine parvovirus, Bovine bath.
adenovirus 3 and 5, Rabies virus, Blue tongue, Bovine
syncitial respiratory virus (BRSV) and Reovirus. 2. Preheat a water bath to 56°C.
3. Agitate the bottles to mix the contents. Set them into
In addition, each lot receives our full cell growth test the water bath so that the water level is above serum
following irradiation and a Certificate of Irradiation is level.
provided confirming the product received the proper dose 4. Place a thermometer in a bottle with an equivalent
required to inactivate bovine viruses. volume of water in the water bath as a control.
Special Serum Treatments 5. Mix the serum occasionally as the temperature rises.
Monitor the temperature of the water bath to ensure
We will heat inactivate or gamma irradiate serum on an that it stays at 56°C. When the temperature of the
individual basis. Customers may designate approved lots water in the control bottle reaches 56°C, set a timer
to be heat inactivated or gamma irradiated by informing for 30 minutes.
customer service when placing an order. There will be an 6. At regular intervals, ensure that the temperature is
additional charge for these services. Please allow 4 weeks or 56°C and swirl the bottles.
more, for these procedures.
7. At the end of 30 minutes, remove the bottles from the
Special Handling Fees water bath and label them as “heat inactivated”.
NOTE: Unless there is a compelling reason to heat inactivate your FBS, we
Heat Inactivation Irradiation recommend that it not be done. Heat inactivation was historically done for
many reasons that are no longer valid. Generally, FBS performs better in
$3.00/2,50 € per 50 ml $11.00/9,00 € per 50 ml cell culture without heat treatment.
$3.00/2,50 € per 100 ml $11.00/9,00 € per 100 ml
$6.00/5,00 € per 500 ml $11.00/9,00 € per 500 ml
$6.00/5,00 € per 1,000 ml $11.00/9,00 € per 1,000 ml
Minimum Charge: Minimum Charge:
NOTE: Please contact your local Lonza office or your local distributor for
pricing outside the US or Europe.
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Fetal Bovine Sera
Fetal Bovine Serum Premium, US Origin Fetal Bovine Serum Premium, US Origin, Heat Inactivated
Screened for viruses by full compliance 9CFR (113.53) and the absence of Heat inactivated at 56°C for 30 minutes; screened for viruses by
mycoplasmas; 3 × 0.1-micron filtered full compliance 9CFR (113.53) and the absence of mycoplasmas;
Storage Conditions: -10°C – -20°C 3 × 0.1-micron filtered
Storage Conditions: -10°C – -20°C
14-501E 100 ml
14-503E 100 ml
14-501F 500 ml
14-503F 500 ml
Fetal Bovine Serum, USDA Approved Source
Screened for viruses by full compliance 9CFR (113.53)and the absence of Fetal Bovine Serum Premium, US Origin,
mycoplasmas; 3 × 0.1-micron filtered
Storage Conditions: -10°C – -20°C
Hybridoma Screened
Screened to support hybridoma growth; screened for viruses by
full compliance 9CFR (113.53) and the absence of mycoplasmas;
14-502E 100 ml 3 × 0.1-micron filtered
14-502F 500 ml Storage Conditions: -10°C – -20°C
14-901E 100 ml
Fetal Bovine Serum, USDA Approved Source, 14-901F 500 ml
Heat Inactivated
Heat inactivated at 56°C for 30 minutes; screened for viruses by full
compliance 9CFR (113.53) and the absence of mycoplasmas; 3 × Fetal Bovine Serum Premium, US Origin, for Testing
0.1-micron filtered Chlamydia
Storage Conditions: -10°C – -20°C Screened to support chlamydia growth; contains 10X L-glutamine and
10X nystatin, a chlamydia isolation component; screened for viruses
14-507E 100 ml by full compliance 9CFR (113.53) and the absence of mycoplasmas;
14-507F 500 ml 3 × 0.1-micron filtered
Storage Conditions: -10°C – -20°C
14-471E 100 ml
14-471F 500 ml
Fetal Bovine and
Equine Sera
Equine Sera
Ordering Information
14-403E 100 ml
14-403F 500 ml
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Bovine Sera
14-492E 100 ml
14-492F 500 ml
Human Sera
Ordering Information
Cat. No. Size Price
Human Serum
14-402E 100 ml
Human Serum, AB
Storage Conditions: -10°C – -20°C
14-490E 100 ml
14-498E 100 ml
14-491E 100 ml
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Fetal Bovine Serum (Available Outside USA and Canada)
The following sera are ONLY available for customers outside the USA and Canada
All sera listed follow EC regulations on import for serum Ordering Information
into the European community. Products manufactured
Cat. No. Size Price
using those sera might be subject to import restrictions
in some countries (e.g. USA). Fetal Bovine Serum, EU Standard (Low IgG, IgG content
lower than 100 microgram/ml)
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and
The EC approved fetal bovine sera are manufactured in a the absence of mycoplasma, 0.2 µm filtered
European ISO 9001 certified facility. Storage Conditions: -10°C – -20°C
DE14-870E 100 ml
■ Finished Product Quality Control
DE14-870F 500 ml
Samples of each lot of finished product are selected and
tested in accordance with written product specifications. Fetal Bovine Serum, EU Standard (Delipidized)
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and
Final product quality control includes the following: the absence of mycoplasma, 0.2 µm filtered
Storage Conditions: -10°C – -20°C
– Sterility – Tests are performed on representative DE14-840E 100 ml
samples using a modification of the membrane DE14-840F 500 ml
filtration method described in the EP Pharmacopoeia
– Mycoplasma – Final products are tested for myco- Fetal Bovine Serum, EU Standard (ES qualified serum)
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and
plasma using the culture test described European the absence of mycoplasma, 0.2 µm filtered
Storage Conditions: -10°C – -20°C
Pharmacopoeia (EP)
– Adventitious Viruses – All fetal bovine serum lots receive DE14-850E 100 ml
■ Applications:
– Support growth of undifferentiated embryonic stem
cells
– Low unspecific stimulation
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Fetal Bovine Serum (Available Outside USA and Canada)
Continued
Fetal Bovine Serum EU Standard (UltraLow IgG, Fetal Bovine Serum EU Standard, Dialyzed
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and
<8 mg/ml) the absence of mycoplasma. Dialyzed against physiological NaCl solution
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and or PBS using a 10 kDa molecular weight cut-off membrane until the glucose
the absence of mycoplasma, 0.2 µm filtered level is < 10 mg/dl. To prevent precipitation and inactivation of serum peptides
Storage Conditions: -10°C – -20°C we do not perform exhaustive dialysis. Thus we do not certify that all low
molecular weight dialyzable components, such as amino acids, are totally
DE14-860F 500 ml removed by our processing. The dialyzed serum is sterile filtered using
0.2 µm filter.
Storage Conditions: -10°C – -20°C
Fetal Bovine Serum EU Standard
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and
DE14-810E 100 ml
the absence of mycoplasma, 0.2 µm filtered
Storage Conditions: -10°C – -20°C DE14-810F 500 ml
DE14-801E 100 ml
Fetal Bovine Serum EU Standard, Charcoal Dextran
DE14-801F 500 ml
Treated
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and
Fetal Bovine Serum EU Standard, Research Grade the absence of mycoplasma, Charcoal Dextran treated, 0.2 µm filtered
South American origin, screened for viruses (IBR, PI3 and BVD) and the absence Storage Conditions: -10°C – -20°C
of mycoplasma, 0.2 µm filtered
Storage Conditions: -10°C – -20°C DE14-820E 100 ml
DE14-820F 500 ml
DE14-802E 100 ml
DE14-802F 500 ml
Fetal Bovine Serum EU Standard, Tetracycline Free
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and
Fetal Bovine Serum, USDA Country of Origin the absence of mycoplasma, 0.2 µm filtered
Australian origin, screened for viruses (IBR, PI3 and BVD) and the absence Storage Conditions: -10°C – -20°C
of mycoplasma, 0.2 µm filtered
Storage Conditions: -10°C – -20°C DE14-830E 100 ml
DE14-830F 500 ml
DE14-701E 100 ml
DE14-701F 500 ml
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Cell Culture Technical Information
We offer an extensive line of cell culture media and of the commonly asked questions relating to cell culture
reagents backed by years of experience and innovation. techniques by providing instructions and tips for adapting
Through our own internal efforts as well as work with cultures to serum-free medium, cryopreservation and
exceptional collaborators, we are able to provide ongoing reconstitution, preparing powdered media, and more. Our
technical support in the form of protocols, detailed product Scientific Support Department is prepared to assist you
information, and troubleshooting tips for our broad range with many other technical questions and concerns related
of media and reagents. In this section we address many to cell culture and media.
ensure that the subpopulation selected exhibits the same outcome. We recommend that the cells be maintained
characteristics with respect to cellular function as the above 3.0 × 105/ml for attachment independent and above
population that was cultivated in the presence of serum. 30% confluency for attachment dependent cells.
These functions are diverse and may include receptor
expression, viral susceptibility, monoclonal antibody
production, and recombinant gene expression. In many
cases, an increase in product yield has been noted when
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Protocols for Weaning Cell Cultures
Protocol #1: Medium Replacement – for adherent Protocol #2: Serum Dilution – for adherent dependent
independent cells (suspensions) cells
Approximate time required: 2 weeks – 6 weeks Approximate time required: 2 weeks–6 weeks
Culture conditions: Culture conditions:
– Mammalian cells: 95% air, 5% CO2, 35°C – 37°C – Mammalian cells: 95% air, 5% CO2, 35°C – 37°C
– Invertebrate cells: air, 25°C – 27°C – Invertebrate cells: air, 25°C – 27°C
1. Begin with cultures at maximum cell density. 1. Begin with cultures at maximum cell density.
note: Attachment dependent cells that are exposed to trypsin during
subculturing should be converted to serum-free growth using Protocol #2.
2. Trypsinize attachment dependent cultures and
transfer to an appropriately sized centrifuge tube.
2. Split cells 1:2 using serum-free medium as the Attachment independent cells may be transferred
diluent. directly to the centrifuge tube.
3. Incubate cells until the maximum cell density is 3. Sediment the cells by centrifugation at 350 × g for
achieved. 5 minutes.
4. Split cells 1:5 or to 3.0 × 105 cells/ml for attachment 4. Resuspend the cells in serum-free medium containing
independent cells or 30% confluency for attachment 5% serum (v/v).
dependent cells using serum-free medium as the
diluent. 5. Adjust the cell concentration using the serum
supplemented serum-free medium to a maximum of
5. Incubate cells until the maximum cell density is 3.0 × 105 cells/ml for attachment independent cells or
achieved. a density to achieve not less than 30% confluency for
6. If the cell viability is >85% at this point, and the attachment dependent cells.
generation time is similar to that observed with serum- 6. Plant the cells and incubate until a maximum cell
containing medium, the culture may be maintained in density is achieved.
serum-free medium using a similar split schedule as
originally optimized for serum-containing medium. 7. Repeat steps 2 – 6 using a lower concentration of
serum at each split. We recommend beginning at 5%
7. If the cells exhibit slow growth or low viability, maintain serum and lowering to 2%, 1%, 0.5%, and finally 0.1%
the split ratio at 1:2 or 1:5 for 3 successive splits. The prior to eliminating serum from the culture.
minimum cell density should be above 3.0 × 105 cells/ml
or 30% confluency during this period. note: If the culture viability drops below 80% or if the generation time
increases markedly following a decrease in the serum concentration,
8. Gradually increase the split ratio to obtain a maximum increase the serum level to the previous value and maintain the cells for
2 split cycles before lowering the level of serum again. It may be necessary
value for the cell type being used. to institute a more gradual decline in serum concentration with these cells.
Some cell types may require a small amount of serum for growth. If the
note: Some cells may require a small amount of serum for growth. If the cells have not adapted to serum-free cultivation using the protocol described
cells have not adapted to serum-free cultivation using the above protocol, above, add 0.1 – 0.5% serum to the culture or contact Scientific Support at
add 0.1% – 0.5% serum to the culture or contact Scientific Support at scientific.support.eu@lonza.com.
scientific.support.eu@lonza.com.
Protocols for Weaning
Cell Cultures
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Cryopreservation and Reconstitution
4. Add the above cell suspension to an equal volume of 1. Remove an ampoule from the freezer and place it into
cold (+4°C) Cryoprotective Freezing Medium (Cat. No. a 37°C waterbath. Do not submerge the ampoule or
12-132A). allow water to get under the cap.
5. Mix continuously to ensure homogeneity. 2. After thawing, disinfect the ampoule with 70%
isopropanol, then open aseptically in a laminar flow
6. Dispense either 4 ml of the cells suspended in safety cabinet. Dilute the contents 1:10 in the appropriate
Cryoprotective Freezing Medium into 5 ml glass growth medium.
ampoules, or 1 ml into plastic screw cap vials suitable
for freezing in the liquid or vapor phase of liquid 3. Determine the viability and cell concentration of the
nitrogen. thawed cells by using the Trypan Blue exclusion cell
counting method.
7. Cells are now ready for the freezing cycle. Cells should
not be allowed to remain in the Cryoprotective Freezing 4. Adjust the cell concentration as desired for seeding
Medium for more than 1 hour before freezing. culture vessels.
8. The temperature of the contents in the ampoule 5. Twenty-four hours after cells have been seeded, remove
must then be lowered at a rate of 0.5°C – 2°C/minute the medium and refeed with the appropriate growth
throughout the range of +4°C to -30°C. medium. This will remove the cryopreservative, if the
alternative method described below is not used.
9. After the temperature has reached -30°C, the rate of
the temperature drop to -70°C (which is the warmest 6. As an alternative, the cryopreservative may be removed
temperature at which cells can be stored) can be prior to cell viability determination by centrifugation.
done very quickly. An automatic programmable This is done by centrifuging the resuspended cells at
freezer system is the most reliable means of obtaining low speed for 10 minutes (200 × g). The supernatant is
controlled rate freezing. removed and the cells are resuspended in the appropriate
growth medium.
10. Storage of the ampoules or vials must be at -70°C
or colder. A storage temperature of -196°C (liquid
nitrogen) is best. It is essential that the temperature Reference
of the contents of the ampoule be -70°C or colder Freshney, R.I. (2000) Culture of Animal Cells: A Manual of Basic Technique,
4th edition, Wiley-Liss, Inc., New York, pp. 297 – 308.
at all times until reconstitution. For prolonged or
indefinite storage, the use of liquid nitrogen is strongly
Cryoprerservation and
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Determination of Cell Numbers
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Determination of Cell Numbers
Continued
Materials Procedure
1. Clean hemacytometer and cover glass 1. Dilute 0.2 ml of Trypan Blue with 0.8 ml of HBSS.
2. Pasteur pipettes 2. Place cover glass over hemacytometer chamber.
3. Hanks’ Balanced Salt Solution (HBSS) 3. Transfer 0.5 ml of agitated cell suspension to a 12 × 75
(Cat. No. 10-543) mm tube and add 0.5 ml of diluted Trypan Blue.
4. Trypan Blue, 0.4% in BSS (Cat. No. 17-942E) 4. With Pasteur pipette, fill both chambers of the
5. Microscope hemacytometer (without overflow) by capillary action.
Cells will settle in the tube and in the pipette by gravity
6. Tubes (12 × 75 mm) within a few seconds. Work quickly.
7. Hand counter 5. Using a microscope with a 10X ocular and a 10X
8. Cell suspension objective, count the cells in each of 10 squares (1 mm2
each). If over 10% of the cells represent clumps, repeat
entire sequence. If fewer than 200 or more than 500
cells are present in the 10 squares, repeat with a more
suitable dilution factor.
6. Calculate the number of cells per ml, and total number of
cells in the original culture as follows:
Cells/ml = average count per square × 104 × dilution factor (i.e., 2, if 0.5 ml
of cells plus 0.5 ml of Trypan Blue is used)
Total cells = cells/ml × total volume of cell preparation from which sample
was taken
7. Repeat count to check reproducibility.
Determination of
Cell Numbers
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Powdered Media Preparation
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Subculturing Procedures for Mammalian Cells
NOTE: Not applicable to Clonetics® and Poietics® Primary Human or Animal Trypsinization Procedure
Cells.
In cell culture there is frequently the need to subculture Cell cultures are normally subcultured (“split”) when the
cells. In doing so, cells can be propagated for the purposes cultures are at or near confluency. As a general rule, the
of increasing cell numbers or providing cells in a culture longer the time frame between when confluency is first
vessel suitable to one’s needs. There are a number of ways achieved and subculturing, the longer it will take for the
to remove cells from one culture vessel and pass them to trypsin to act.
another vessel. Cells may be removed from surfaces on 1. Decant medium from the culture vessel. Serum
which they are attached by: inhibits trypsin activity, so complete removal of
serum-containing medium is necessary.
1. Mechanical means (scraping)
2. Rinse the cell sheet with BSS without calcium
2. Chelating agents, ethylenediaminetetraacetic acid and magnesium before addition of Trypsin/Versene®
[EDTA] (Cat. No. 17-161). The monolayer should be thoroughly
3. Enzymes (trypsin, pronase, collagenase) covered with BSS. This rinse is instantaneous but the
Enzymes and chelating agents are often used in combi- BSS can remain on the cell sheet for up to 4 hours, if
nation. Trypsin is an aqueous crude extract prepared desired.
from porcine pancreas. It is the most common means 3. Pour off rinse medium. Trypsin/Versene® is to be added
used for removal of cells from surfaces and from intact to each vessel as follows:
tissue. Trypsin is, to some extent, a misnomer because 75 cm2 flask 2.5 ml to 5.0 ml
in addition to trypsin, the preparation contains other 150 cm2 flask 5.0 ml to 10.0 ml
proteases, lipases, and carbohydrases. The multitude 850 cm2 roller bottle 10.0 ml to 20.0 ml
of digestive enzymes produced by the pancreas would 4. Cover the monolayer thoroughly with Trypsin/Versene®.
be expected to be found in trypsin preparations. Pure Since different lots of Trypsin/Versene® may vary in
crystalline trypsin can be used, but it is more expensive strength, it is acceptable to monitor the trypsinization
than crude trypsin and often does not work as well, process at room temperature for the first 30 seconds.
especially when preparing cells from intact tissue. This will ensure that the trypsinization process is not
occurring too rapidly.
The optimum conditions for trypsin activity are a pH
range of 7.6 – 7.8 and a temperature of 37°C. The effect 5. The culture vessel should then be moderately hit
of trypsin is to break down the intracellular matrix that against the palm of the hand to see if the cells are
binds cells to each other or to a substrate surface. being dislodged. Hold the vessel up to a light in a
vertical position and look for signs of the cell sheet
There are no chemical standards for trypsin activity. We sloughing off of the surface. If the entire monolayer is
conduct quality assurance tests on trypsin to determine dislodged, proceed to step #6. If not, incubate at 37°C
its capacity to detach cells from a substrate surface in a and observe the vessel every minute for dissociation.
standard time period without damage. This is in addition The culture vessel should again be hit against the palm
to the usual tests for sterility. of the hand to ensure all cells have been dislodged.
Remove culture vessel from the incubator.
Trypsin is typically used at concentrations between 6. Immediately transfer dissociated cells to a vessel
0.05% and 0.25%, although some applications may containing medium supplemented with 10% serum. All
Subculturing Procedures
require concentrations outside this range. Versene® of the cells should be removed. Aspirate the medium
for Mammalian Cells
(EDTA) enhances trypsin action, and therefore lowers the plus cells with a pipette onto the surface to remove
required trypsin concentration for effective performance. all remaining cells. It is essential that this aspiration
Concentrated trypsin (2.5%, Cat. No. 17-160) should be be done as completely as possible with a small bore
diluted in calcium- and magnesium-free balanced salt pipette so as to obtain individual, dispersed cells. If the
solution (BSS) (Hanks’ BSS, Cat. No. 10-543; or Dulbecco’s cells are not separated, the new culture will contain
Phosphate Buffered Saline, Cat. No. 17-512). Dilution in numerous microcolonies. Cells added to the vessel
water is not recommended since the solution will be should be stirred with a magnetic stir bar at a speed
hypotonic and produce cell damage. Dilution in saline that avoids vortexing (approximately 100 – 200 rpm),
alone is also damaging to cells.
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Subculturing Procedures for Mammalian Cells
Continued
Trypsinization Procedure, continued the number of population doublings that have elapsed.
or agitated frequently. It is important at this point to With a 1:2 split ratio this is achieved by simply adding
add medium containing serum at least 10 times the “1” to each split since this ratio yields one population
volume of Trypsin/Versene® used. This will ensure that doubling. Larger split ratios can be used. For example,
the digestive agent is inhibited. a split ratio of 1:4 would yield 2 doublings per 1:4 split;
a 1:8 split ratio would yield 3 doublings per 1:8 split. In
7. Add sufficient fresh medium to the aspirated order to have knowledge of the approach of cessation,
suspension so that the total volume will cover the it is essential to keep records of the number of elapsed
surface of two culture vessels, each having the same population doublings.
surface area as the original culture vessel (or use a
single culture vessel having twice the floor area of the 15. Since human diploid cells multiply by fission, the
original vessel). This is a 1:2 split. Other split ratios increase in population may be expressed per cell as
can be used for vigorously growing cell populations. follows:
1 2 4 8 16 …Number of cells
8. Incubate the culture vessel (or vessels) at 37°C. 0 1 2 3 4 …Population Doubling Level (PDL)
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Cell Culture FAQs
Media FAQs
Q. Are all SFM (serum-free media) protein-free? Q. If medium is accidentally frozen, will it still
function properly?
A. No, this is a common misconception.
Q. What is chemically defined, serum-free medium? A. In most cases medium that is frozen should
not be adversely affected. Upon thawing the
A. Chemically defined media contain only medium should be examined for precipitate.
components of known molecular structure. If any evidence of precipitation is present, the
Constituents such as extracts or hydrolysates medium should be incubated in a 37°C water
are examples of undefined materials. bath for a few minutes. The bottles should be
gently swirled while in the water bath and the
Q. What are the advantages of using serum-free precipitate should go back into solution. If the
media? precipitate remains, the medium should be
A. – Reduces media variables discarded.
– Eliminates the expense and time required Q. Can you manufacture custom media formu-
to perform the extensive testing required by lations?
most labs to qualify new lots of serum A. Yes, Lonza provides custom media formulations
in bottles and bags of various sizes. If you
– Improves cell performance consistency
are interested in a custom production, please
– Eases downstream protein purification contact your sales representative.
Q. Does Lonza manufacture a serum-free medium Q. Why might customers want phenol red-free
that supports the growth of a wide variety of media?
cell lines?
A. Phenol red is a somewhat variable material
A. UltraCULTURE™ Medium (12-725F) is a complete shown to contain trace impurities that can mimic
serum-free medium designed for the cultivation the effects of estrogen. Some customers want
of a wide variety of mammalian cell types to avoid the risk of potential adverse impact of
including adherent and non-adherent primary these estrogen-like effects.
cells and established cell lines. Q. Can I expect comparable results using
Q. Can media products be used past their expiration ProFreeze™-CDM Medium as compared to
date? Why do certain media products not have serum-containing cryopreservation cocktails?
an expiration date? A. ProFreeze™-CDM Medium achieves these same
A. We do not recommend the use of a product past protective results, but with a chemically defined
its expiration date. Lonza has an ongoing shelf- formulation that contains no animal derived
life monitoring program that supports the labeled components. ProFreeze™-CDM Freezing Medium
expiration dates for its products. is universally suitable for cryopreserving many
cell types in the absence of fetal bovine serum
(FBS). However, it is used to greatest advantage
Products that are labeled for Research Use are with cells cultured in a serum-free and animal
non-In Vitro Diagnostic (IVD) products and are component-free environment. This protein-free
not required to be labeled with an expiration freezing medium contains no animal derived
date. Establishing appropriate shelf-life may components, insulin, or hydrolysate, and
be problematic because of the variety of maintains high cell viability upon recovery from
applications for which these products are used. frozen storage.
Lonza labels non-IVD products with the date of
Cell Culture FAQs
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Cell Culture FAQs
Continued
Sera FAQs
Q. The medium I purchased from Lonza already Q. What is the difference between Fetal Bovine
contains L-glutamine. Should I further supplement Serum (FBS)-USA and FBS-USDA?
the medium with additional L-glutamine prior to
A. FBS-USA are sera collected from USDA approved
use? sites within the United States. FBS-USDA are
A. Further supplementation of L-glutamine is not sera collected from USDA approved sites outside
necessary if the medium is used within expiration the United States.
period and stored according to product label. See Q. Is FBS tested for viruses?
the L-glutamine technical data sheet for more
information on the web at https://bcprd.lonza. A. Yes, all lots are full 9CFR 113.53 (c) [113.46,
com/shop/deeplink/area.do?area=1133510 113.47] tested for Bovine Viral Diarrhea (BVD),
Parainfluenza virus type 3 (P13), Bovine rhino
Q. What is the difference between L-glutamine and tracheitis (IBR), Bovine parvovirus, Bovine
UltraGlutamine? adeno 3, Bovine adeno 5, Rabies virus, Blue
A. UltraGlutamine is an extremely stable dipeptide tongue, Bovine respiratory syncytial virus
form of L-glutamine that avoids accumulation (BRSV), and Reovirus.
of toxic ammonia produced by spontaneous Q. What is the difference between calf serum and
degradation in the medium. Applications that newborn calf serum?
may benefit from UltraGlutamine are ammonia
sensitive systems (bioreactors), long-term A. Newborn calf serum is obtained from animals up
toxicity studies, and long incubations without to 10 days old. Calf serum is obtained from calves
feeding (cloning assays). 10 days to six months of age and weighing up to
200 kg.
Q. What are your endotoxin and hemoglobin
specifications for FBS?
A. We sell only Type I FBS. Our specifications for
FBS are endotoxin ≤10 EU/ml and hemoglobin
of ≤20 mg/deciliter. Typical endotoxin and
hemoglobin results of our FBS are undetected
and 6 – 9 mg/deciliter, respectively. Type II sera
have endotoxin and hemoglobin above these
threshold levels. Endotoxin and hemoglobin
levels exceeding these threshold levels are
indicative of poor handling and processing
practices. Endotoxin impacts over 30 biological
activities and can lead to cell death.
Q. Do I need to heat inactivate the fetal bovine
serum?
A. Unless you have a compelling scientific
reason to heat inactivate your FBS, we would
not recommend doing it. Heat inactivation was
historically done for many reasons that are no
longer valid. Generally, FBS performs better in
cell culture without heat treatment.
Cell Culture FAQs
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Cell Culture FAQs
Continued
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Formulations for BioWhittaker® Classical Media
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Formulations for BioWhittaker® Classical Media
Continued
Nicotinamide 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
Pyridoxine•HCl 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
Riboflavin 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40
Thiamine•HCl 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
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Formulations for BioWhittaker® Classical Media
Continued
Choline Chloride 8.98 8.98 8.98 Folic Acid 1.32 1.32 1.32
Folic Acid 2.66 2.66 2.66 i-Inositol 18.00 0.54 0.54
i-Inositol 12.51 12.50 12.50 Nicotinamide 0.037 0.620 0.62
Nicotinamide 2.02 2.02 2.02 Pyridoxine•HCl 0.062 0.210 0.21
Pyridoxine•HCl 2.03 2.03 2.03 Riboflavin 0.038 0.380 0.38
Riboflavin 0.22 0.22 0.22 Thiamine•HCl 0.34 1.01 1.01
Thiamine•HCl 2.17 2.17 2.17 Vitamin B12 1.36 1.36 1.36
Vitamin B12 0.68 0.68 0.68
1. Ham, R.G. (1965) Proc. Natl. Acad. Sci. USA 53: 288 – 293.
2. Ham, R.G. (1963) Exp. Cell Res. 29:515 – 526.
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Continued
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Formulations for BioWhittaker® Classical Media
Continued
Iscove’s Modified Dulbecco’s Medium (IMDM) L-15 (Leibovitz) Medium McCoy’s 5A Medium
Cat. No. 12-722 12-726 12-915 Cat. No. 12-669 12-700 Cat. No. 12-168 12-688
Liquid Liquid Liquid 2X Liquid Liquid Liquid Liquid
COMPONENT mg/L mg/L mg/L COMPONENT mg/L mg/L COMPONENT mg/L mg/L
INORGANIC SALTS INORGANIC SALTS INORGANIC SALTS
CaCl2 (anhyd.) 165.00 165.00 165.00 CaCl2(anhyd.) 280.00 140.00 CaCl2 (anhyd.) 100.00 100.00
KCl 330.00 330.00 330.00 KCl 800.00 400.00 KCl 400.00 400.00
KNO3 0.076 0.076 0.076 KH2PO4 120.00 60.00 MgSO4•7H2O 200.00 200.00
MgSO4 (anhyd.) 97.66 97.67 97.67 MgCl2 anhyd.) 187.34 93.67 NaCl 5,460.00 6,460.00
NaCl 4,505.00 4,505.00 4,505.00 MgSO4 (anhyd.) 195.46 97.70 NaHCO3 2,200.00 2,200.00
NaHCO3 3,024.00 3,024.00 3,024.00 NaCl 16,000.00 8,000.00 NaH2PO4•H2O 580.00 580.00
NaH2PO4•H2O 125.00 125.00 125.00 Na2HPO4 (anhyd.) 380.00 190.00 OTHER COMPONENTS
Na2SeO3•5H20 0.01 0.01 0.01 OTHER COMPONENTS Bactopeptone 600.00 600.00
OTHER COMPONENTS D(+) Galactose 1,800.00 900.00 Glucose 3,000.00 3,000.00
Glucose 4,500.00 4,500.00 4,500.00 Phenol Red•Na 10.00 Glutathione (reduced) 0.50 0.50
HEPES 5,958.00 5,958.00 5,958.00 Sodium Pyruvate 1,100.00 550.00 HEPES 5,957.40
Phenol Red•Na 15.00 15.00 15.00 AMINO ACIDS Phenol Red•Na 10.00 10.00
Sodium Pyruvate 110.00 110.00 110.00 DL-Alanine 900.00 450.00 AMINO ACIDS
AMINO ACIDS L-Arginine 1,000.00 500.00 L-Alanine 13.36 13.36
L-Alanine 25.00 25.00 25.00 L-Asparagine 500.00 250.00 L-Arginine•HCl 42.14 42.14
L-Arginine•HCl 84.00 84.00 84.00 L-Cysteine 240.00 120.00 L-Asparagine•H2O 45.03 45.03
L-Asparagine•H2O 28.40 28.40 28.40 Glycine 400.00 200.00 L-Aspartic Acid 19.97 19.97
L-Aspartic Acid 30.00 30.00 30.00 L-Histidine 500.00 250.00 L-Cysteine•HCl•H2O 35.12 35.12
L-Cystine•2HCl 91.24 91.24 91.24 DL-Isoleucine 500.00 250.00 L-Glutamic Acid 22.07 22.07
L-Glutamic Acid 75.00 75.00 75.00 L-Leucine 250.00 125.00 L-Glutamine 219.15 219.15
L-Glutamine 584.00 584.00 L-Lysine 150.00 75.00 Glycine 7.51 7.51
Glycine 30.00 30.00 30.00 DL-Methionine 300.00 150.00 L-Histidine•HCl•H2O 20.96 20.96
L-Histidine•HCl•H2O 42.00 42.00 42.00 DL-Phenylalanine 500.00 250.00 Hydroxy L•Proline 19.67 19.67
L-Isoleucine 105.00 105.00 105.00 L-Serine 400.00 200.00 L-Isoleucine 39.36 39.36
L-Leucine 105.00 105.00 105.00 DL-Threonine 1,200.00 600.00 L-Leucine 39.36 39.36
L-Lysine•HCl 146.00 146.00 146.00 L-Tryptophan 40.00 20.00 L-Lysine•HCl 36.54 36.54
L-Methionine 30.00 30.00 30.00 L-Tyrosine•2Na•2H2O 370.00 370.00 L-Methionine 14.92 14.92
L-Phenylalanine 66.00 66.00 66.00 DL-Valine 400.00 200.00 L-Phenylalanine 16.52 16.52
L-Proline 40.00 40.00 40.00 VITAMINS L-Proline 17.27 17.27
L-Serine 42.00 42.00 42.00 DL-Ca Pantothenate 2.00 1.00 L-Serine 26.28 26.28
L-Threonine 95.00 95.00 95.00 Choline Chloride 2.00 1.00 L-Threonine 17.87 17.87
L-Tryptophan 16.00 16.00 16.00 Folic Acid 2.00 1.00 L-Tryptophan 3.06 3.06
L-Tyrosine•2Na•2H2O 104.20 104.20 104.20 i-Inositol 4.00 2.00 L-Tyrosine 18.12 18.12
L-Valine 94.00 94.00 94.00 Nicotinamide 2.00 1.00 L-Valine 17.57 17.57
VITAMINS Pyridoxine•HCl 2.00 1.00 VITAMINS
d-Biotin 0.013 0.013 0.013 Riboflavin-5-PO4•Na 0.20 0.10 p-Aminobenzoic Acid 1.00 1.00
D-Ca Pantothenate 4.00 4.00 4.00 Thiamine 2.00 1.00 Ascorbic Acid 0.50 0.50
Choline Chloride 4.00 4.00 4.00 •Monophosphate d-Biotin 0.20 0.20
Folic Acid 4.00 4.00 4.00 Leibovitz:, A. (1963) Am. J. Hyg. 78: D-Ca Pantothenate 0.20 0.20
i-Inositol 7.20 7.20 7.20 173 – 180. Choline Chloride 5.00 5.00
Nicotinamide 4.00 4.00 4.00 Folic Acid 10.00 10.00
Pyridoxine•HCl 4.00 4.00 4.00 i-Inositol 36.00 36.00
Riboflavin 0.40 0.40 0.40 Nicotinamide 0.50 0.50
Thiamine•HCl 4.00 4.00 4.00 Nicotinic Acid 0.50 0.50
Vitamin B12 0.013 0.013 0.013 Pyridoxal•HCl 0.50 0.50
Iscove, N.N. and F. Melchers (1978) J. of Exptl.Med.147: Pyridoxine•HCl 0.50 0.50
923 – 933. Riboflavin 0.20 0.20
Thiamine•HCl 0.20 0.20
Vitamin B12 2.00 2.00
McCoy, T.A., M. Maxwell, and P.F. Kruse, Jr.
Formulations for
Classical Media
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Formulations for BioWhittaker® Classical Media
Continued
Folic Acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 2.00
i-Inositol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 4.00
Nicotinamide 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 2.00
Pyridoxal•HCl 1.00 1.00 1.00 1.00 2.00
Pyridoxine•HCl 1.00 1.00 1.00 1.00 1.00 1.00
Riboflavin 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.20
Thiamine•HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 2.00
Vitamin B12 1.33
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Formulations for BioWhittaker® Classical Media
Continued
L-Tyrosine 16.40
L-Valine 25.00
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Formulations for BioWhittaker® Classical Media
Continued
RPMI Medium
— Europe Only —
Cat. No. 09-774 12-115 12-167 12-918 12-702 04-525 15-702 BE12-752 BE12-115/U1 BE12-702/U1
Liquid Liquid Liquid Liquid Liquid Liquid Powder Liquid Liquid Liquid
COMPONENT mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L
INORGANIC SALTS
Ca(NO3)2•4H2O 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00
KCl 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00
MgSO4 (anhyd.) 48.83
MgSO4•7H2O 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00
NaCl 5,000.00 5,000.00 6,000.00 6,000.00 6,000.00 6,000.00 6,000.00 6,000.00 5,000.00 6,000.00
NaHCO3 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00
Na2HPO4 800.49
Na2HPO4•7H2O 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00
OTHER COMPONENTS
Glucose 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00
Glutathione (reduced) 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
HEPES 5,957.50 5,957.40 5,957.50
MOPS 34,535.00
Penicillin G Potassium (units/ml) 100
Phenol Red•Na 5.00 5.00 5.00 5.00 5.00 5.10 5.00 5.00 5.00
Streptomycin (mcg/ml) 50.00
AMINO ACIDS
L-Arginine 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00
L-Arginine•HCl 241.86
L-Asparagine•H2O 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00
L-Aspartic Acid 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Cystine•2HCl 65.19
L-Cystine 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00
L-Glutamic Acid 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Glutamine 300.00 300.00 300.00 300.00 300.00 300.00
L-Alanyl-L-Glutamine (UltraGlutamine 1) 446.00 446.00
Glycine 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00
L-Histidine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00
L-Histidine•HCl•H2O 20.27
Hydroxy L•Proline 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Isoleucine 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00
L-Leucine 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00
L-Lysine•HCl 40.00 40.00 40.00 40.00 40.00 40.00 40.00 40.00 40.00 40.00
L-Methionine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00
L-Phenylalanine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00
L-Proline 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Serine 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00
L-Threonine 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Tryptophan 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00
L-Tyrosine 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Tyrosine•2Na 28.83
L-Valine 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
VITAMINS
p-Aminobenzoic Acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
d-Biotin 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20
D-Ca Pantothenate 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
Choline Chloride 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00
Folic Acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
i-Inositol 35.00 35.00 35.00 35.00 35.00 35.00 35.00 35.00 35.00 35.00
Formulations for
Classical Media
Nicotinamide 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
Pyridoxine•HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
Riboflavin 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20
Thiamine•HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
Vitamin B12 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
Moore, G.E., R.E. Gerner, and H.A. Franklin (1967) J. Am. Med. Assoc. 199:87 – 92.
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Antibiotic Solutions
Cat. No. 09-757 17-518 17-519 17-602 17-603 17-718 17-719 17-745 17-836 02-012
17-528
COMPONENT/UNITS Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid
Amphotericin B (mg/L) 25.00 250.00
Gentamicin sulfate (mg/L) 50,000.00 10,000.00 10,000.00
L-Glutamine (mg/L) 29,200.00
NaCl (mg/L) 8,500.00 8,500.00 8,500.00 8,500.00
Potassium Penicillin 20,000.00 10,000.00 5,000.00 25,000.00 25,000.00 10,000.00
(units/ml)
Streptomycin sulfate 20,000.00 10,000.00 5,000.00 25,000.00 25,000.00 10,000.00
(mcg/ml)
Lymphocyte Separation
Balanced Salt Solutions (EBSS and HBSS) Medium
— EBSS Europe Only — — HBSS — Cat. No. 17-829
Cat. No. 10-502 02-027 04-315 10-508 10-527 10-543 10-547 Liquid
Liquid Liquid Liquid Liquid Liquid Liquid Liquid COMPONENT mg/L
COMPONENT mg/L mg/L mg/L mg/L mg/L mg/L mg/L Ficoll 400 62,000.00*
INORGANIC SALTS Sodium Diatrizoate 104,000.00
CaCl2•2H2O 265.00 265.00 186.0 186.0 Specific Gravity 1.077 g/ml
KCl 400.00 400.00 400.00 400.00 400.00 400.00 400.00 *Slight adjustments may be required
KH2PO4 60.00 60.00 60.00 60.00 60.00 to obtain the specified specific
MgSO4•7H2O 200.00 200.00 200.00 200.00 gravity
NaCl 6,800.00 6,800.00 8,000.00 8,000.00 8,000.00 8,000.00 8,000.00
NaHCO3 2,200.00 1,800.00 350.00 350.00 350.00 350.00 350.00
Na2HPO4•2H2O 158.00 158.00 MEM Non-essential
Na2HPO4•7H2O 90.00 90.00 90.00 90.00 90.00 Amino Acids
OTHER COMPONENTS
Glucose (anhyd.) 1,000.00 1,000.00 1,000.00 1,000.00 1,000.00 Cat. No. 13-114
Glucose•H2O 1,100.00 1,100.00 Liquid
HEPES 4,766.00 COMPONENT mg/L
Phenol Red•Na 10.60 10.60 20.00 20.00 L-Alanine 890.00
L-Asparagine 1,321.00
L-Aspartic Acid 1,330.00
L-Glutamic Acid 1,470.00
Glycine 750.00
Dulbecco’s Phosphate Buffered Saline (DPBS) L-Proline 1,150.00
L-Serine 1,050.00
Cat. No. 04-479 17-512 17-513 17-515
Liquid Liquid Liquid Liquid 10X
COMPONENT mg/L mg/L mg/L mg/L MEM Vitamin Solution
INORGANIC SALTS
CaCl2•2H2O 130.00 130.00 Cat. No. 13-607
KCl 200.00 200.00 200.00 2,000.00 Liquid
KH2PO4 200.00 200.00 200.00 2,000.00 COMPONENT mg/L
MgCl2•6H2O 100.00 100.00 D-Ca Pantothenate 100.00
MgCl2 (anhyd.) Choline Chloride 100.00
NaCl 8,000.00 8,000.00 8,000.00 80,000.00 Folic Acid 100.00
Na2HPO4 i-Inositol 200.00
Na2HPO4•7H2O 2,160.00 2,160.00 2,160.00 21,600.00 Nicotinamide 100.00
OTHER COMPONENTS Pyridoxine•HCl 100.00
Glucose 1,000.00 Riboflavin 10.00
Formulations for
Classical Media
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Cell Culture Reagent Formulations
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Notes
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Endotoxin Detection
Chapter 6
Endotoxin Detection
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Endotoxin Detection
Fast and easy endotoxin detection for your research
Endotoxin is a pyrogen from Gram-negative bacteria. As an industry leader in endotoxin testing sales
Endotoxin impacts over 30 biological activities (including and technology, Lonza offers a wide variety of
macrophage activation, mitogenic activity, induction of assays for endotoxin detection. For the research
interferon and colony stimulating factor). Endotoxin can customer, we have included in this catalog Gel Clot
lead to cell death by intitiating complement activation. LAL (PYROGENT®), Endpoint Chromogenic LAL
(QCL-1000®), and PyroGene® Recombinant Factor
Endotoxin Detection
Blood cells (amebocytes) from horseshoe crabs were C Assays, as well as instrumentation, software and
found to clot in the presence of endotoxin and this accessories to run these assays.
technology was used in the development of endotoxin
detection assays. The Limulus Amebocyte Lysate (LAL) In addition, Lonza offers kinetic quantitative assays and a
test was commercially introduced in the 1970’s. More Testing Service. More information can be found on the web
recently, Lonza scientists have produced a recombinant at www.lonza.com or call Scientific Support.
form of Factor C, the first component in the Limulus
polyphemus clotting cascade activated by endotoxin.
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Gel Clot LAL Assays
endotoxin. For your convenience, the Certificate of PYROGENT® Plus Gel Clot LAL Assay
Analysis documenting the FDA and USP required RSE/ Contains:
1 ✕ 10 ng/vial endotoxin
CSE correlation is available at www.lonza.com/coa. 64 Tests = 4 ✕ 16 tests/vial of lysate
PYROGENT® Plus Kits are available with sensitivities of 200 Tests = 4 ✕ 50 tests/vial of lysate
0.03, 0.06, 0.125 and 0.25 EU/ml. Standard kit sizes are N294-03 200 tests 0.03
200 tests, 64 tests, and a 24-test kit in a single test vial N283-06 64 tests 0.06
format is available. N294-06 200 tests 0.06
■ Storage Conditions N283-125 64 tests 0.125
N294-125 200 tests 0.125
2°C – 8°C
N284-25 200 tests 0.25
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Gel Clot LAL Assays
2°C – 8°C
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Fluorescent Assay
time zero and after a one-hour incubation in a fluorescent For instrumentation and software see page 324
microplate reader using excitation/emission wavelengths
of 380/440 nm.
Advantages of PyroGene® Assay:
— Greater endotoxin specificity The rFC Assay and LAL Assays measure similar endotoxin
— Elimination of false positive glucan reactions potency
Relative Endotoxin Potency
— Less lot-to-lot variability Kinetic-QCL® Assay
2.0 PyroGene® Assay
Fluorescent Assay
The PyroGene® Assay represents a reliable, 1.5
■ Storage Conditions
2°C – 8°C 0.5
1e+5
Lot 061101
Lot 051401
1e+4 Lot 111201
Lot 041601
1e+3
1e+2
1e+1
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LAL Accessory Products
Ordering Information
Name Cat. No. Size Storage Conditions Comments Price
Reagents
b-G-Blocker N190 5 ✕ 5 ml vials 2°C – 8°C Used to block glucan interference
PYROSPERSE® Dispensing Agent N188 5 ✕ 5 ml vials 2°C – 30°C Dispersing agent
MgCl2 S50-641 30 ml vial 2°C – 30°C 10 mM Solution
Tris Buffer S50-642 30 ml vial 2°C – 30°C 50 mM Solution
LAL Reagent Water W50-640 30 ml 2°C – 8°C <0.005 EU/ml
W50-100 100 ml 15°C – 30°C <0.005 EU/ml
W50-500 500 ml 15°C – 30°C <0.005 EU/ml
Ordering Information
Name Cat. No. Size Quantity Comments Price
Labware
Pyrogen-free Test Tubes N201 10 ✕ 75 mm 50/pkg w/polypropylene screw caps
Pyrogen-free Test Tubes N205 10 ✕ 75 mm 50/pkg Foil wrapped without caps
LAL Accessory Products
Pyrogen-free Dilution Tubes N207 13 ✕ 100 mm 30/pkg Foil wrapped without caps
Reagent Reservoir 25-364 — 10/pkg For use with 8-channel pipettes
Sample Container, 80-507L 10 ml 25/box Glass bottles with screw caps
Depyrogenated 80-507U 100/box
Eppendorf® Biopur 25-415 200 µl 5 trays/pkg;
Pipette Tips 96 tips/tray
25-416 300 µl Only for multichannel pipettors
25-417 1,000 µl
Stepped Neutral 25-342 — For use in ELx808LBS plate readers
Density Plate
Ground Aluminum 25-038 — Each Holds a 96-well plate in a dry bath
Block with Lid multiblock heater
LAL Reagent Grade Plates 25-340 — 50/case 96-well, <0.0005 EU/well
Ordering Information
Name Cat. No. Comments Price
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Flexible Bioprocess Containers
Chapter 7
Flexible Bioprocess Containers
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Flexible Bioprocess Containers
Lonza is committed to providing innovative products durability and flexibility. Aside from having superior
for the life science community. Our Platinum UltraPAK™ gas barrier properties, undetectable endotoxin, and
Bioprocess Containers are no exception, providing the certification as a USP Class VI material, our film is
best single-use container for your bioprocessing needs. non-animal in origin (NAO). As a result, our film provides
complete protection and reliability to many products that
Flexible bioprocess containers are the efficient alternative come in contact with it such as:
Flexible Bioprocess
packaging to traditional, rigid wall containers used for the
■ Cell culture media
transfer of liquid media, buffers, or product intermediates.
Containers
Platinum UltraPAK™ Single-use, Pillow-style Containers ■ Buffers
are available in multiple configurations using a 4-web ■ Water for Injection (WFI)
proprietary film. For the past 10 years, Lonza has
■ Final product yielded from downstream manufacturing
continually produced high quality, flexible packaging
for the pharmaceutical and biotech community. Lonza processes
bioprocess containers offer extensive customization We chose a four-web, polyethylene film design, providing
capabilities with a large variety of connectors and tubing more flexibility and less rigidity compared to single-web
types, while maintaining a short turn around time for films, without sacrificing durability. More rigid films have
custom made bags. a tendency to generate pinhole leaks.
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Flexible Bioprocess Containers
Physiochemical Assay – The physical and chemical Brittleness Temperature Test – Samples of irradiated
properties of the film and its extracts, using the and artificially aged irradiated film were subjected to
Non-volatile Residue, Residue on Ignition, Heavy Metals –80˚C in accordance with ASTM D1790-02. The Platinum
and Buffering Capacity Assays, were determined UltraPAK™ Film showed no visible evidence of damage
according to the protocol referenced from USP <661> when subjected to this rigorous test.
Containers Physiochemical Tests-Plastics.
Freezing Integrity Study – To ensure the durability of
Flexible Bioprocess
In vitro and In vivo Biological Reactivity Tests – Samples our flexible packaging as a complete system for frozen
of irradiated and artificially aged irradiated film were applications and conditions, bioprocess containers
Containers
assayed for biological reactivity with mammalian cell produced with irradiated Platinum UltraPAK™ Film were
culture and biological response of animals following the filled with Trypticase Soy Broth. The filled systems were
requirements of Class VI plastics, as specified in USP incubated at two separate temperatures: (1) at 20° C - 25°C
<87> Biological Reactivity Test, in vitro and in vivo. for two weeks followed by; (2) 30°C - 35°C for an additional
two weeks, to demonstrate sterility prior to freezing.
Moisture Vapor Transmission Rate – We demonstrated After incubation, the filled bioprocess containers were
the barrier properties of irradiated and artificially aged frozen to – 20°C and incubated a second time at the two
irradiated film by determining the Rate of Water Vapor separate temperature ranges and durations as described
Transmission (in grams/100 square inches/day). The previously. Integrity and sterility were maintained during
quantitative data was determined by performing the this rigorous test with no evidence of contamination.
protocol referenced from ASTM F1249-01.
Polyethylene with Additives for Containers for Parenteral
Oxygen and Carbon Dioxide Transmission Rate – We Preparation EP 5.4, 2006, 3.1.5 – Irradiated bags were
demonstrated the barrier properties of irradiated and tested to meet the requirements of the European
artificially aged irradiated film by determining the Rate of Pharmacopoeia regarding “polyethylene with additives for
Oxygen and Carbon Dioxide Transmission (in grams/100 containers for parenteral preparation and for ophthalmic
square inches/day). The quantitative data was determined preparations”. The study was based upon the European
by performing the protocol referenced from ASTM D3985. Pharmacopoeia current version: Chapter 3.1.5. Based on
the evaluation criteria, Platinum UltraPAK™ Bioprocess
Heat Seal and Fitment Seal Strength – To ensure the Containers meet the requirements of the EP current
strength of a heat sealed seam (either the film to itself version, Chapter 3.1.5.
or the film to a port), we performed an assay referenced
from ASTM D882. To test the heat seal strength, all four Plastic Containers for Parenteral Infusion EP 5.4, 2006,
sides of a pillow-style flexible container were assayed. 3.2.2.1 – Irradiated bags were tested to meet the
The data demonstrated superior strength. requirements of the European Pharmacopoeia regarding
plastic containers for aqueous solutions for parenteral
Integrity Testing – To ensure the durability of our flexible infusion. Based on the evaluation criteria, Platinum
packaging as a complete system, bioprocess containers UltraPAK™ Bioprocess Containers meet the requirements
produced with irradiated and artificially aged irradiated of the EP 5.4, 2006, Chapter 3.2.2.1.
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Flexible Bioprocess Containers
Flexible Bioprocess
– Left Seal 27.57 lb/in 29.76 lb/in
Fitment Seal Strength
Containers
– Width 41.7 lb/in 26.4 lb/in
– Length 28.3 lb/in 39.0 lb/in
– Perpendicular 102.4 lb/in 105.0 lb/in
Shipping Integrity Test Sterile Sterile
Brittleness Temperature Test -80° C, no evidence of visible damage -80° C, no evidence of visible damage
Freezing Integrity Test Integrity of the bag is kept; no visual signs of N/A
contamination of the medium after incubation
Polyethylene with additives for Meets EP 3.1.5 N/A
containers for parenteral preparation
and for ophthalmic preparations
Plastic containers for aqueous solutions Meets EP 3.2.2.1 N/A
for parenteral infusion
{
Polyetheylene
EVOH
Outer film layer Polyethylene
construction Tie layer
PVDC
Nylon
The Platinum UltraPAK™ Film fluid contact layer is a high quality polyethylene.
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Flexible Bioprocess Containers
Port 2: 18" of 1/4" PVC tubing with male MPC with female cap
— Robust ports – Lonza's goal was to maximize the
08-E1322 1 L, 25 bags per case
Containers
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Flexible Bioprocess Containers
— Non-Animal Origin – The film is completely non-animal 08-E275 560 L, 10 bags per 30" ✕ 48"
case
origin (NAO), including slip agents used in the 08-E276 790 L, 10 bags per 38" ✕ 48"
manufacturing process case
— Integrity Tested – Complete systems were rigorously 08-E277 1,090 L, 10 bags per 42" ✕ 48"
case
tested to ensure shipping stability and durability
08-E278 1,360 L, 10 bags per 48" ✕ 48"
— Brittleness Temperature Tested – Subjected to -80°C case
Flexible Bioprocess
in accordance with ASTM D1790-02
Gusseted-Style Tank liners
— Broad Range of Sizes – Pillow-style tank liners range
Containers
08-E282 50 L, 20 bags per case 13.5” x 29”
in size from 50 L to 1360 L, while our gusseted tank
08-E283 100 L, 20 bags per 18.5" ✕ 33"
liners range in size from 50 L to 200 L case
— Convenient Single-Use – Eliminates cross-contami- 08-E284 200 L, 20 bags per 22.5" ✕ 38"
case
nation; cleaning of dedicated equipment and cleaning
validation
— Gamma Irradiated – Off-the-shelf ready; no in-plant
sterilization needed
— Flexibility – Tank liners provide quick scale-up and
scale-down; reduces loss time between manufacturing
campaigns
— Minimal Training – Nominal operator training and
preparation time
All bioprocess tank liners are manufacturing in a cGMP,
FDA Registered, class 10,000 clean room to ensure
product consistency and quality assurance. Quality
testing includes complete cGMP traceability and exceeds
ISO 9001 standards.
The LLDPE film is tested for thickness, seal properties,
and bond strength prior to manufacturing. A thorough
visual inspection is conducted on tank liner film after
manufacturing and prior to irradiation.
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Flexible Bioprocess Containers
ProEVA PAK™ 1 L
Lonza ProEVA PAK™ Bioprocess Containers have the Port 1: male luer with 3/16" barb
Port 2: female luer with 3/16" barb
following features: Port 3: stays the same break-off injection site
— Ethylene-Vinyl Acetate – Product contact layer and a 08-E287 1 L, 25 units per case
polyethylene/EVOH barrier layer that offers durability
ProEVA PAK™ 5 L, 10 L, and 20 L
and superior gas barrier properties
Port 1: male MPC with 1/4" barb
— Unmatched Versatility in Connectivity – Our standard Port 2: male MPC with 1/4" barb
Port 3: female luer with 3/16" barb with male luer cap and built in injection
systems offer MPC and luer connections, along with site
needleless injection sites for sampling 08-E279 5 Liters, 20 units per case
— Continuous Sealing Process – Generated by our auto- 08-E280 10 Liters, 20 units per case
Flexible Bioprocess
mated sealing process, eliminating possible seam 08-E281 20 Liters, 20 units per case
leaks
Containers
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Custom Services
Chapter 8
Lonza Custom Manufacturing
— Recombinant proteins
— Cell-based therapeutics
— Drug conjugates
— Vaccines
— Plasmid DNA
— Peptides
— Oligonucleotides
— Fine chemicals
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Lonza Custom Manufacturing
Slough Verviers
Braine Kourim
Conshohocken Visp
Portsmouth
Porriño
Walkersville Hopkinton
Nansha
Custom Services
Singapore
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Transfection Services
■ Protocol optimization for either your Nucleofector® II For more information about protocol optimization or
Device or your 96-well Shuttle® System development of stably transfected cells, please contact
■ Generation of fully characterized stable transfectants your local Sales Representative.
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Indices
Chapter 9
Numerical Index
Cat. No. Description Page Number Cat. No. Description Page Number
50568 Transport™ Reagent 400 µg . . . . . . . . . . . . . . . . . . . . . . 202 00188235 Huntington’s Disease 2 x 96-well . . . . . . . . . . . . . . . . . .212
77201 HL-1™ Liquid w/o L-Glut 2 × 500 ml . . . . . . . . . . . . . . . . 274 00188236 Immune Complement 2 x 96-well . . . . . . . . . . . . . . . . .212
77204 HL-1™ Powder Kit w/o L-Glut, 50 L . . . . . . . . . . . . . . . . . 274 00188237 Immune System Phenotyping 2 x 96-well . . . . . . . . . .212
77227 HL-1™ Supplement 100X 10 ml . . . . . . . . . . . . . . . . . . . . 274 00188238 Immunological Targets of NFKB 2 x 96-well . . . . . . . . .212
77232 PC-1™ Liquid 2 × 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 263 00188239 Innate Immune Signaling 2 x 96-well . . . . . . . . . . . . . .212
00188072 Alzheimer’s Disease 2 x 96-well . . . . . . . . . . . . . . . . . .214 00188240 Interferon Signaling & Response 2 x 96-well . . . . . . .212
00188073 Antigen Processing & Presentation 2 x 96-well . . . . .214 00188241 Mood Disorder 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . .212
00188074 Apoptosis 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .214 00188242 Neurodegeneration 2 x 96-well . . . . . . . . . . . . . . . . . . . .213
00188075 Autophagy 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .214 00188243 NFKB Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . .213
00188076 B cell Developmental and Function 2 x 96-well . . . . .214 00188244 Notch Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . .213
00188077 B cell Receptor and Signaling 2 x 96-well . . . . . . . . . . .214 00188245 Obesity 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .213
00188078 Blood Coagulation 2 x 96-well . . . . . . . . . . . . . . . . . . . . .214 00188246 Osteoporosis 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . .213
00188079 Cell Cycle Tox and Cancer 2 x 96-well . . . . . . . . . . . . . .214 00188247 p53 Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . .213
00188139 Cytokines and Receptors 2 x 96-well . . . . . . . . . . . . . .214 00188248 Parkinson’s 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . .213
00188140 DNA Damage and Repair 2 x 96-well . . . . . . . . . . . . . . .214 00188249 Stress Response 2 x 96-well . . . . . . . . . . . . . . . . . . . . . .213
00188161 Electron Transport Toxicology 2 x 96-well . . . . . . . . . .214 00188250 T cell Receptor Signaling 2 x 96-well . . . . . . . . . . . . . . .213
00188162 FoxP3 Target Genes 2 x 96-well . . . . . . . . . . . . . . . . . . .214 00188251 T helper 17 (Th17) 2 x 96-well . . . . . . . . . . . . . . . . . . . . .213
00188163 General Toxicology 2 x 96-well . . . . . . . . . . . . . . . . . . . .214 00188252 T Regulatory Phenotyping 2 x 96-well . . . . . . . . . . . . .213
00188164 Growth and Development Tox 2 x 96-well . . . . . . . . . . .215 00188253 Targets of Wnt/ß-catenin Signaling 2 x 96-well . . . . .213
00188165 Hematopoetic Cell Lineage 2 x 96-well . . . . . . . . . . . . .215 00188254 TGF-ß Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . .213
00188166 Huntington’s Disease 2 x 96-well . . . . . . . . . . . . . . . . . .215 00188255 Toll-like Receptor Signaling 2 x 96-well . . . . . . . . . . . . .213
00188167 Immune Complement 2 x 96-well . . . . . . . . . . . . . . . . .215 00188256 Wnt Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . .213
00188168 Immune System Phenotyping 2 x 96-well . . . . . . . . . .215 00188257 Alzheimer’s Disease 2 x Fast 96-well . . . . . . . . . . . . . 211
00188169 Immunological Targets of NFKB 2 x 96-well . . . . . . . . .215 00188258 Antigen 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . . . 211
00188170 Innate Immune Signaling 2 x 96-well . . . . . . . . . . . . . .215 00188259 Apoptosis 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . 211
00188171 Interferon Signaling & Response 2 x 96-well . . . . . . .215 00188260 Autophagy 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . 211
00188172 Mood Disorder 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . .215 00188261 B cell Receptor and Signaling 2 x Fast 96-well . . . . . 211
00188173 Natural Killer Cell 2 x 96-well . . . . . . . . . . . . . . . . . . . . . .215 00188262 Blood Coagulation 2 x Fast 96-well . . . . . . . . . . . . . . . 211
00188174 Neurodegeneration 2 x 96-well . . . . . . . . . . . . . . . . . . . .215 00188263 Cell Cycle Tox and Cancer 2 x Fast 96-well . . . . . . . . . 211
00188183 NFKB Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . .215 00188264 Cytokines and Receptors 2 x Fast 96-well . . . . . . . . . 211
00188184 Notch Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . .215 00188265 DNA Damage and Repair 2 x Fast 96-well . . . . . . . . . .212
00188185 Obesity 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 00188266 Electron Transport Toxicology 2 x Fast 96-well . . . . . .212
00188186 Osteoporosis 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . 216 00188267 FoxP3 Target Genes 2 x Fast 96-well . . . . . . . . . . . . . . .212
00188187 p53 Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . 216 00188268 General Toxicology 2 x Fast 96-well . . . . . . . . . . . . . . . .212
00188188 Parkinson’s 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . 216 00188269 Growth and Development Tox 2 x Fast 96-well . . . . . .212
00188189 Pathogen Recognition 2 x 96-well . . . . . . . . . . . . . . . . 216 00188270 Hematopoetic Cell Lineage 2 x Fast 96-well . . . . . . . .212
00188190 Stress Response 2 x 96-well . . . . . . . . . . . . . . . . . . . . . 216 00188271 Huntington’s Disease 2 x Fast 96-well . . . . . . . . . . . . .212
00188191 T cell Receptor Signaling 2 x 96-well . . . . . . . . . . . . . . 216 00188272 Immune Complement 2 x Fast 96-well . . . . . . . . . . . . .212
00188192 T helper 17 (Th17) 2 x 96-well . . . . . . . . . . . . . . . . . . . . 216 00188273 Immune System Phenotyping 2 x Fast 96-well . . . . .212
00188193 T Regulatory Phenotyping 2 x 96-well . . . . . . . . . . . . 216 00188274 Immunological Targets of NFKB 2 x Fast 96-well . . . .212
00188194 Targets of Wnt/ß-catenin Signaling 2 x 96-well . . . . 216 00188275 Innate Immune Signaling 2 x Fast 96-well . . . . . . . . . .212
00188195 TGF-ß Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . 216 00188276 Interferon Signaling & Response 2 x Fast 96-well . . .212
00188196 Toll-like Receptor Signaling 2 x 96-well . . . . . . . . . . . . 216 00188277 Mood Disorder 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . .212
00188197 Wnt Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . 216 00188278 Neurodegeneration 2 x Fast 96-well . . . . . . . . . . . . . . .213
00188199 Alzheimer’s Disease 2 x 384-well . . . . . . . . . . . . . . . . . .214 00188279 NFKB Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . .213
00188200 Diabesity 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .214 00188280 Notch Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . .213
00188201 General Toxicology 2 x 384-well . . . . . . . . . . . . . . . . . . .214 00188281 Obesity 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . . . .213
00188202 Immunology 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . .215 00188282 Osteoporosis 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . .213
00188203 Lymphoma and Leukemia 2 x 384-well . . . . . . . . . . . .215 00188283 p53 Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . .213
00188204 Mood Disorder 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . .215 00188284 Parkinson’s 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . .213
00188205 Stem Cell 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . 216 00188285 Stress Response 2 x Fast 96-well . . . . . . . . . . . . . . . . .213
00188211 Alzheimer’s Disease 2 x 96-well . . . . . . . . . . . . . . . . . . 211 00188286 T cell Receptor Signaling 2 x Fast 96-well . . . . . . . . . .213
00188212 Antigen 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 00188287 T helper 17 (Th17) 2 x Fast 96-well . . . . . . . . . . . . . . . .213
00188213 Apoptosis 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 00188288 T Regulatory Phenotyping 2 x Fast 96-well . . . . . . . . .213
00188214 Autophagy 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 00188289 Targets of Wnt/ß-catenin Signaling 2 x Fast 96-well 213
00188215 B cell Receptor and Signaling 2 x 96-well . . . . . . . . . . 211 00188290 TGF-ß Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . .213
00188216 Blood Coagulation 2 x 96-well . . . . . . . . . . . . . . . . . . . . 211 00188291 Toll-like Receptor Signaling 2 x Fast 96-well . . . . . . . .213
Indices
00188217 Cell Cycle Tox and Cancer 2 x 96-well . . . . . . . . . . . . . 211 00188292 Wnt Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . .213
00188218 Cytokines and Receptors 2 x 96-well . . . . . . . . . . . . . 211 00188293 Alzheimer’s Disease 2 x 384-well . . . . . . . . . . . . . . . . . 211
00188219 DNA Damage and Repair 2 x 96-well . . . . . . . . . . . . . . .212 00188294 Antigen 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211
00188220 Electron Transport Toxicology 2 x 96-well . . . . . . . . . .212 00188295 Apoptosis 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . 211
00188231 FoxP3 Target Genes 2 x 96-well . . . . . . . . . . . . . . . . . . .212 00188296 Autophagy 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . 211
00188232 General Toxicology 2 x 96-well . . . . . . . . . . . . . . . . . . . .212 00188297 B cell Receptor and Signaling 2 x 384-well . . . . . . . . 211
00188233 Growth and Development Tox 2 x 96-well . . . . . . . . . . .212 00188298 Blood Coagulation 2 x 384-well . . . . . . . . . . . . . . . . . . . 211
00188234 Hematopoetic Cell Lineage 2 x 96-well . . . . . . . . . . . . .212 00188299 Human Cell Cycle Tox and Cancer 2 x 384-well . . . . . 211
344
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Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
00188300 Cytokines and Receptors 2 x 384-well . . . . . . . . . . . . 211 00188394 Parkinson’s 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . 216
00188301 DNA Damage and Repair 2 x 384-well . . . . . . . . . . . . . .212 00188395 Pathogen Recognition 2 x Fast 96-well . . . . . . . . . . . . 216
00188302 Electron Transport Toxicology 2 x 384-well . . . . . . . . .212 00188396 Stress Response 2 x Fast 96-well . . . . . . . . . . . . . . . . 216
00188303 FoxP3 Target Genes 2 x 384-well . . . . . . . . . . . . . . . . . .212 00188397 T cell Receptor Signaling 2 x Fast 96-well . . . . . . . . . 216
00188304 General Toxicology 2 x 384-well . . . . . . . . . . . . . . . . . . .212 00188398 T helper 17 (Th17) 2 x Fast 96-well . . . . . . . . . . . . . . . 216
00188305 Growth and Development Tox 2 x 384-well . . . . . . . . .212 00188399 T Regulatory Phenotyping 2 x Fast 96-well . . . . . . . . 216
00188306 Hematopoetic Cell Lineage 2 x 384-well . . . . . . . . . . . .212 00188400 Targets of Wnt/ß-catenin Signaling 2 x Fast 96-well 216
00188307 Huntington’s Disease 2 x 384-well . . . . . . . . . . . . . . . . .212 00188401 TGF-ß Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . 216
00188308 Immune Complement 2 x 384-well . . . . . . . . . . . . . . . .212 00188402 Toll-like Receptor Signaling 2 x Fast 96-well . . . . . . . 216
00188309 Immune System Phenotyping 2 x 384-well . . . . . . . .212 00188403 Wnt Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . 216
00188310 Immunological Targets of NFKB 2 x 384-well . . . . . . . .212 00188424 Alzheimer’s Disease 2 x 384-well . . . . . . . . . . . . . . . . . .214
00188311 Innate Immune Signaling 2 x 384-well . . . . . . . . . . . . .212 00188425 Antigen Processing & Presentation 2 x 384-well . . . .214
00188312 Interferon Signaling & Response 2 x 384-well . . . . . .212 00188426 Apoptosis 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . .214
00188313 Mood Disorder 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . .212 00188427 Autophagy 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . .214
00188314 Neurodegeneration 2 x 384-well . . . . . . . . . . . . . . . . . .213 00188428 B cell Developmental and Function 2 x 384-well . . . .214
00188315 NFKB Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .213 00188429 B cell Receptor and Signaling 2 x 384-well . . . . . . . . .214
00188316 Notch Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .213 00188430 Blood Coagulation 2 x 384-well . . . . . . . . . . . . . . . . . . . .214
00188317 Obesity 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . .213 00188431 Cell Cycle Tox and Cancer 2 x 384-well . . . . . . . . . . . . .214
00188318 Osteoporosis 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . .213 00188432 Cytokines and Receptors 2 x 384-well . . . . . . . . . . . . .214
00188319 p53 Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . .213 00188433 DNA Damage and Repair 2 x 384-well . . . . . . . . . . . . . .214
00188320 Parkinson’s 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . .213 00188434 Electron Transport Toxicology 2 x 384-well . . . . . . . . .214
00188321 Stress Response 2 x 384-well . . . . . . . . . . . . . . . . . . . . .213 00188435 FoxP3 Target Genes 2 x 384-well . . . . . . . . . . . . . . . . . .214
00188322 T cell Receptor Signaling 2 x 384-well . . . . . . . . . . . . . .213 00188436 General Toxicology 2 x 384-well . . . . . . . . . . . . . . . . . . .214
00188323 T helper 17 (Th17) 2 x 384-well . . . . . . . . . . . . . . . . . . .213 00188437 Growth and Development Tox 2 x 384-well . . . . . . . . .215
00188324 Human T Regulatory Phenotyping 2 x 384-well . . . . .213 00188438 Hematopoetic Cell Lineage 2 x 384-well . . . . . . . . . . . .215
00188325 Targets of Wnt/ß-catenin Signaling 2 x 384-well . . . .213 00188439 Huntington’s Disease 2 x 384-well . . . . . . . . . . . . . . . . .215
00188326 TGF-ß Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .213 00188440 Immune Complement 2 x 384-well . . . . . . . . . . . . . . . .215
00188327 Toll-like Receptor Signaling 2 x 384-well . . . . . . . . . . .213 00188441 Immune System Phenotyping 2 x 384-well . . . . . . . .215
00188328 Wnt Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . .213 00188442 Immunological Targets of NFKB 2 x 384-well . . . . . . . .215
00188329 Alzheimer’s Disease 2 x 384-well . . . . . . . . . . . . . . . . . 211 00188443 Innate Immune Signaling 2 x 384-well . . . . . . . . . . . . .215
00188330 Diabesity 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 00188444 Interferon Signaling & Response 2 x 384-well . . . . . .215
00188331 General Toxicology 2 x 384-well . . . . . . . . . . . . . . . . . . .212 00188445 Mood Disorder 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . .215
00188332 Immunology 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . .212 00188446 Natural Killer Cell 2 x 384-well . . . . . . . . . . . . . . . . . . . .215
00188333 Lymphoma and Leukemia 2 x 384-well . . . . . . . . . . . .212 00188447 Neurodegeneration 2 x 384-well . . . . . . . . . . . . . . . . . .215
00188334 Mood Disorder 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . .212 00188448 NFKB Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .215
00188335 Stem Cell 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .213 00188449 Notch Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .215
00188365 Alzheimer’s Disease 2 x Fast 96-well . . . . . . . . . . . . . .214 00188450 Obesity 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . 216
00188366 Antigen Processing & Presentation 2 x Fast 96-well 214 00188451 Osteoporosis 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . 216
00188367 Apoptosis 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . .214 00188452 p53 Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . 216
00188368 Autophagy 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . .214 00188453 Parkinson’s 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . 216
00188369 B cell Developmental and Function 2 x Fast 96-well 214 00188454 Pathogen Recognition 2 x 384-well . . . . . . . . . . . . . . . 216
00188370 B cell Receptor and Signaling 2 x Fast 96-well . . . . . .214 00188455 Stress Response 2 x 384-well . . . . . . . . . . . . . . . . . . . . 216
00188371 Blood Coagulation 2 x Fast 96-well . . . . . . . . . . . . . . . .214 00188456 T cell Receptor Signaling 2 x 384-well . . . . . . . . . . . . . 216
00188372 Cell Cycle Tox and Cancer 2 x Fast 96-well . . . . . . . . . .214 00188457 T helper 17 (Th17) 2 x 384-well . . . . . . . . . . . . . . . . . . 216
00188373 Cytokines and Receptors 2 x Fast 96-well . . . . . . . . . .214 00188458 T Regulatory Phenotyping 2 x 384-well . . . . . . . . . . . 216
00188374 DNA Damage and Repair 2 x Fast 96-well . . . . . . . . . .214 00188459 Targets of Wnt/ß-catenin Signaling 2 x 384-well . . . 216
00188375 Electron Transport Toxicology 2 x Fast 96-well . . . . . .214 00188460 TGF-ß Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . 216
00188376 FoxP3 Target Genes 2 x Fast 96-well . . . . . . . . . . . . . . .214 00188461 Toll-like Receptor Signaling 2 x 384-well . . . . . . . . . . 216
00188377 General Toxicology 2 x Fast 96-well . . . . . . . . . . . . . . . .214 00188462 Wnt Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . 216
00188378 Growth and Development Tox 2 x Fast 96-well . . . . . .215 00188657 AMPK Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . 211
00188379 Hematopoetic Cell Lineage 2 x Fast 96-well . . . . . . . .215 00188658 AMPK Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . 211
00188380 Huntington’s Disease 2 x Fast 96-well . . . . . . . . . . . . .215 00188659 AMPK Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . 211
00188381 Immune Complement 2 x Fast 96-well . . . . . . . . . . . . .215 00188660 AMPK Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .214
00188382 Immune System Phenotyping 2 x Fast 96-well . . . . .215 00188661 AMPK Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . .214
00188383 Immunological Targets of NFKB 2 x Fast 96-well . . . .215 00188662 AMPK Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . .214
00188384 Innate Immune Signaling 2 x Fast 96-well . . . . . . . . . .215 00189269 PowerCHO® -GS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268
00188385 Interferon Signaling & Response 2 x Fast 96-well . . .215 00189910 WinKQCL® 4 Software Package . . . . . . . . . . . . . . . . . . . 330
Indices
00188386 Mood Disorder 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . .215 04-351Q Schneider’s Drosophila 1 L . . . . . . . . . . . . . . . . . . . . . . . 259
00188387 Natural Killer Cell 2 x Fast 96-well . . . . . . . . . . . . . . . . .215 04-380Q X-VIVO™ 10 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
00188388 Neurodegeneration 2 x Fast 96-well . . . . . . . . . . . . . . .215 04-409R PBS-1X 15 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
00188389 NFKB Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . .215 04-418Q X-VIVO™ 15 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
00188390 Notch Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . .215 04-448Q X-VIVO™ 20 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
00188391 Obesity 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . . . .215 04-457F Grace’s Insect Medium 500 ml . . . . . . . . . . . . . . . . . . . 255
00188392 Osteoporosis 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . 216 04-479Q DPBS (1X) w/Ca++ and Mg++ 1 L . . . . . . . . . . . . . . . . . . . 286
00188393 p53 Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . 216 04-501Q Grace’s Complete Insect 1 L . . . . . . . . . . . . . . . . . . . . . . 255
345
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Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
04-525F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-167F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-585P Water for Cell Culture 20 L . . . . . . . . . . . . . . . . . . . . . . . 284 12-167F/12 RPMI 1640 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-616F TL HEPES Buffer 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 287 12-167F/24 RPMI 1640 24 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-648F Richter’s CM Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 258 12-167Q RPMI 1640 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-649F Grace’s Insect 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 255 12-167Q/12 RPMI 1640 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-719Q MEM Eagle Joklik 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-168F McCoy’s 5A 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
04-743Q X-VIVO™ 10 1 L w/o gentamicin or phenal red . . . . . . 265 12-169F MEM, Alpha 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
04-744Q X-VIVO™ 15 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265 12-604F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 253
04-919Q ProCHO™ 4 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267 12-604F/12 DMEM 4 .5 g/L Glucose 12 x 500ml . . . . . . . . . . . . . . . 253
06-174G MEM Eagle-EBSS 450 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-604Q DMEM 4 .5 g/L Glucose 1 L . . . . . . . . . . . . . . . . . . . . . . . 253
08-003A Hanks’ BSS (1X) 4 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282 12-604Q/12 DMEM 4 .5 g/L Glucose 12 x 1 L . . . . . . . . . . . . . . . . . . . 253
08-012B RPMI 1640 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-611F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
08-199D Water for Cell Culture 100 L . . . . . . . . . . . . . . . . . . . . . . 284 12-611F/12 MEM Eagle EBSS 12 x 500 ml . . . . . . . . . . . . . . . . . . . . 257
08-199E Water for Cell Culture 200 L . . . . . . . . . . . . . . . . . . . . . . 284 12-611Q MEM Eagle EBSS 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
08-199F Water for Cell Culture 20 L . . . . . . . . . . . . . . . . . . . . . . . 284 12-611Q/12 MEM Eagle EBSS 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . 257
08-E1322 Platinum UltraPAK™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-614F DMEM 4 .5 g/L Glucose 500ml . . . . . . . . . . . . . . . . . . . . 253
08-E1323 Platinum UltraPAK™ 5 L . . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-612F/12 DMEM 4 .5 g/L Glucose 12x 500ml . . . . . . . . . . . . . . . . 253
08-E1324 Platinum UltraPAK™ 10 L . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-614Q DMEM 4 .5 g/L Glucose 1 L . . . . . . . . . . . . . . . . . . . . . . . 253
08-E1325 Platinum UltraPAK™ 20 L . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-614Q/12 DMEM 4 .5 g/L Glucose 12x 1 L . . . . . . . . . . . . . . . . . . . 253
08-E269 Pillow Tank Liner 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-615F Ham’s F-12 Med w/L-Gln 500 ml . . . . . . . . . . . . . . . . . . 255
08-E270 Pillow Tank Liner 130 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-618F Ham’s F10 Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 255
08-E271 Pillow Tank Liner 200 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-624E Veronal Buffer (5X) 100 ml . . . . . . . . . . . . . . . . . . . . . . 287
08-E272 Pillow Tank Liner 340 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-662F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
08-E273 Pillow Tank Liner 400 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-662Q MEM Eagle EBSS 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
08-E275 Pillow Tank Liner 560 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-668E MEM Eagle EBSS (2X) 100 ml . . . . . . . . . . . . . . . . . . . . 257
08-E276 Pillow Tank Liner 790 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-669E L-15 (Leibovitz) (2X) 100 ml . . . . . . . . . . . . . . . . . . . . . 256
08-E277 Pillow Tank Liner 1090 L . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-684F MEM Eagle EBSS (10X) 500 ml . . . . . . . . . . . . . . . . . . . 257
08-E278 Pillow Tank Liner 1360 L . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-688F McCoy’s 5A 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
08-E279 ProEVA PAK™ 5 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 12-700F L-15 (Leibovitz) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 256
08-E280 ProEVA PAK™ 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 12-700Q L-15 (Leibovitz) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
08-E281 ProEVA PAK™ 20 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 12-702F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
08-E282 Gusseted Tank Liner 50 L . . . . . . . . . . . . . . . . . . . . . . . . 337 12-702F/12 RPMI 1640 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 259
08-E283 Gusseted Tank Liner 100 L . . . . . . . . . . . . . . . . . . . . . . . 337 12-702Q RPMI 1640 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
08-E284 Gusseted Tank Liner 200 L . . . . . . . . . . . . . . . . . . . . . . . 337 12-702Q/12 RPMI 1640 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
08-E287 ProEVA PAK™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 12-707F DMEM 1 .0 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254
08-E313 Platinum UltraPAK™ 50 L . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-708F DMEM 1 .0 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254
08-E314 Platinum UltraPAK™ 100 L . . . . . . . . . . . . . . . . . . . . . . . . 336 12-709F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254
08-E315 Platinum UltraPAK™ 200 L . . . . . . . . . . . . . . . . . . . . . . . . 336 12-719F DMEM:F12 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 254
09-757F Pen/Strep Stock 500 ml . . . . . . . . . . . . . . . . . . . . 199, 285 12-719Q DMEM:F12 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 254
09-774E RPMI 1640 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-722F IMDM 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
09-774F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-722Q IMDM 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
10-508F Hanks’ BSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282 12-723B UltraDOMA™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 276
10-508Q Hanks’ BSS 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282 12-724Q UltraCHO™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 266
10-539B DGV Buffer 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 12-725F UltraCULTURE™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 262
10-548E ACK Lysing Buffer 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 286 12-726F IMDM 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-029Q ProCHO™ 4 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267 12-726F/12 IMDM 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-105F BME with Earle’s BSS 500 ml . . . . . . . . . . . . . . . . . . . . . 253 12-726Q IMDM 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-109F Medium199 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-726Q/12 IMDM 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-115F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-727F UltraDOMA™-PF™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 277
12-115F/12 RPMI 1640 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-730F Insect-XPRESS™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 278
12-115Q RPMI 1640 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-730Q Insect-XPRESS™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 278
12-115Q/12 RPMI 1640 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-733F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254
12-117F Medium199 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-733Q DMEM 4 .5 g/L Glucose 1 L . . . . . . . . . . . . . . . . . . . . . . . 254
12-117Q Medium199 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-736E MEM Eagle EBSS 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
12-118F Medium199 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-736F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
12-119F Medium 199 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-739F MEM, Glasgow’s 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-125F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
Indices
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Continued
Cat. No. Description Page Number Cat. No. Description Page Number
12-770Q PowerCHO®-1 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 17-516Q PBS (1X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
12-771Q PowerCHO®-2 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 17-516Q/12 PBS (1X) 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
12-772Q PowerCHO®-3 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 17-517Q PBS (10X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
12-773Q ProNS0™ 1 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 17-518L Gentamicin Sulfate 50 mg/ml . . . . . . . . . . . . . . . 199, 285
12-774Q ProNS0™ 2 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 17-518Z Gentamicin Sulfate 50 mg/ml . . . . . . . . . . . . . . . 199, 285
12-775J ProNS0™ Lipid 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 17-519L Gentamicin Sulfate 10 mg/ml . . . . . . . . . . . . . . . 199, 285
12-914F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 253 17-519Z Gentamicin Sulfate 10 mg/ml . . . . . . . . . . . . . . . 199, 285
12-915F IMDM 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255 17-528Z Gentamicin Sulfate 50 mg/ml . . . . . . . . . . . . . . . 199, 285
12-917F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 253 17-602E Pen/Strep stock 100 ml . . . . . . . . . . . . . . . . . . . . 199, 285
12-918F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 17-602F Pen/Strep stock 500 ml . . . . . . . . . . . . . . . . . . . . 199, 285
12-923E NCTC-109 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 17-603E Pen/Strep stock 100 ml . . . . . . . . . . . . . . . . . . . . 199, 285
13-114E MEM NEAA 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283 17-605C L-glutamine 50 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
13-115E Sodium Pyruvate 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 283 17-605E L-glutamine 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
13-607C MEM Eagle Vitamin mix 50 ml . . . . . . . . . . . . . . . . . . . . 283 17-605F L-glutamine 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-401E Calf Serum 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-613E Sodium Bicarb . 7 .5% sol 100 ml . . . . . . . . . . . . . . . . . . 283
14-401F Calf Serum 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-711E Versene® (EDTA), 0 .02% 100 ml . . . . . . . . . . . . . . 46, 284
14-402E Human Serum 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-718R Pen/Strep/L-Gln mixture 25 x 4 .5 ml . . . . . . . . . 199, 285
14-403E Horse Serum, Donor 100 ml . . . . . . . . . . . . . . . . . . . . . . 294 17-719R Pen/Strep stock 25 x 4 .5 ml . . . . . . . . . . . . . . . . . 199, 285
14-403F Horse Serum, Donor 500 ml . . . . . . . . . . . . . . . . . . . . . . 294 17-724F Water for Cell Culture 500 ml . . . . . . . . . . . . . . . . . . . . . 284
14-416E Calf Serum, Newborn 100 ml . . . . . . . . . . . . . . . . . . . . . 295 17-724Q Water for Cell Culture 1 L . . . . . . . . . . . . . . . . . . . . . . . . . 284
14-416F Calf Serum, Newborn 500 ml . . . . . . . . . . . . . . . . . . . . . 295 17-737E HEPES Buffer 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
14-471E FBS Premium Irradiated 100 ml . . . . . . . . . . . . . . . . . . 294 17-737F HEPES Buffer 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
14-471F FBS Premium Irradiated 500 ml . . . . . . . . . . . . . . . . . . 294 17-745E Pen/Strep/Amphotericin B 100 ml . . . . . . . . . . . 199, 285
14-490E Human Serum AB 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 295 17-745H Pen/Strep/Amphotericin B 20 ml . . . . . . . . . . . . 199, 285
14-491E Human Serum AB, Male Plasma Derived 100 ml . . . 295 17-811A UltraCHO™ Supplement 100 ml . . . . . . . . . . . . . . . . . . . 266
14-492E Serum Supreme 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-829E Lymphocyte Separation 100 ml . . . . . . . . . . . . . . . . . . 283
14-492F Serum Supreme 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-829E/24 Lymphocyte Separation 24 x 100 ml . . . . . . . . . . . . . 283
14-498E Human Serum AB, Male 100 ml . . . . . . . . . . . . . . . . . . . 295 17-836E Amphotericin B,antifungal 100 ml . . . . . . . . . . . 199, 285
14-501E FBS Premium 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-836R Amphotericin B,antifungal 20 ml . . . . . . . . . . . . 199, 285
14-501F FBS Premium 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-838Z ITS 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-502E FBS USDA 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-839Z ITES 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-502F FBS USDA 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-905C L-glutamine 50 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-503E FBS Premium HI 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-942E Trypan Blue 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-503F FBS Premium HI 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 294 1A-125 Normal Peripheral Blood Leukopak100 ml . . . . . . . . . 83
14-507E FBS USDA HI 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 1M-101 Bone Marrow CD34+ 100,000 cells . . . . . . . . . . . . . . . . 86
14-507F FBS USDA HI 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 1M-101A Bone Marrow CD34+ 300,000 cells . . . . . . . . . . . . . . . . 86
14-901E FBS Premium Hybr 100 ml . . . . . . . . . . . . . . . . . . . . . . . 294 1M-101B Bone Marrow CD34+ 500,000 cells . . . . . . . . . . . . . . . . 86
14-901F FBS Premium Hybr 500 ml . . . . . . . . . . . . . . . . . . . . . . . 294 1M-101C Bone Marrow CD34+ 1 million cells . . . . . . . . . . . . . . . . 86
14-902E FBS Premium Chy 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 294 1M-105 Human Bone Marrow, Fresh 10 ml . . . . . . . . . . . . . . . . . 83
15-456D DPBS 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 1M-125 Human Bone Marrow, Fresh 25 ml . . . . . . . . . . . . . . . . . 83
15-456F DPBS 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 1M-125A Bone Marrow Mono Cells 200 million cells . . . . . . . . . . 84
15-604D DMEM (Powder) 4 .5 g/L Glucose 10 L . . . . . . . . . . . . 253 1M-125C Bone Marrow Mono Cells 25 million cells . . . . . . . . . . . 84
15-605G L-glutamine Powder 25 g . . . . . . . . . . . . . . . . . . . . . . . . 283 1M-125D Bone Marrow Mono Cells 100 million cells . . . . . . . . . . 84
15-611D MEM Eagle EBSS 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . 257 1M-125E Bone Marrow Mono Cells 300 million cells . . . . . . . . . 84
15-613I Sodium Bicarbonate powder 500 g . . . . . . . . . . . . . . . 283 25-038 Aluminum Block w/lid . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
15-614D DMEM Powder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253 25-315 ELx808LBS Plate Reader . . . . . . . . . . . . . . . . . . . . . . . . 330
15-724D UltraCHO™ 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 266 25-340 LAL grade 96 well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
15-724F UltraCHO™ 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 266 25-342 Stepped Neutral Density Plate . . . . . . . . . . . . . . . . . . . 330
15-727D UltraDOMA™-PF Powder 10 L . . . . . . . . . . . . . . . . . . . . . . 277 25-344 FLx800LBS Fluorescent Plate Reader . . . . . . . . . . . . . 330
17-160E Trypsin(10x) 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284 25-364 Reagent reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
17-160F Trypsin(10x) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284 25-415 Eppendorf® Biopur Tips 200 µl . . . . . . . . . . . . . . . . . . . 330
17-161E Trypsin-Versene® (EDTA) 100 ml . . . . . . . . . . . . . . . . . 284 25-416 Eppendorf® Biopur Tips 300 µl . . . . . . . . . . . . . . . . . . . 330
17-161F Trypsin-Versene® (EDTA) 500 ml . . . . . . . . . . . . . . . . . 284 25-417 Eppendorf® Biopur Tips 1,000 µl . . . . . . . . . . . . . . . . . 330
17-512F DPBS (1X) 500 ml . . . . . . . . . . . . . . . . . . 63, 90 - 91 , 286 2C-101 Cord Blood CD34+ 1 million cells . . . . . . . . . . . . . . . . . . . 87
17-512F/12 DPBS (1X) 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-101A Cord Blood CD34+ 500,000 cells . . . . . . . . . . . . . . . . . . 87
17-512F/24 DPBS (1X) 24 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-101B Cord Blood CD34+ 100,000 cells . . . . . . . . . . . . . . . . . . . 87
17-512Q DPBS (1X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-102 Cord Blood CD133+ 500,000 cells . . . . . . . . . . . . . . . . . 87
Indices
17-512Q/12 DPBS (1X) 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-102A Cord Blood CD133+ 100,000 cells . . . . . . . . . . . . . . . . . 87
17-513F DPBS (1X) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-150 Cord Blood Mononuc Cells 200 million cells . . . . . . . . 84
17-513Q DPBS (1X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-150A Cord Blood Mononuc Cells 100 million cells . . . . . . . . 84
17-515F DPBS (10X) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-200 Cord Blood CD4+ T cells 20 million cells . . . . . . . . . . . . 95
17-515Q DPBS (10X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-250 Cord Blood Erythroid Prog 1 million cells . . . . . . . . . . . 88
17-516F PBS (1X) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46, 287 2C-300 Cord Blood CD19+ B cells 5 million cells . . . . . . . . . . . . 95
17-516F/12 PBS (1X) 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 287 2C-300A Cord Blood CD19+ B Cells 2 .5 million cells . . . . . . . . . . 95
17-516F/24 PBS (1X) 24 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 287 2C-300B Cord Blood CD19+ B Cells 1 million cells . . . . . . . . . . . . 95
347
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Continued
Cat. No. Description Page Number Cat. No. Description Page Number
2L-101A Fetal Liver CD34+ 500,000 cells . . . . . . . . . . . . . . . . . . . 87 AWD-1001 Nucleofector® Guarantee, 1 year ext . . . . . . . . . . . . . . 134
2L-101B Fetal Liver CD34+ 100,000 cells . . . . . . . . . . . . . . . . . . . 87 AWD-1002 Nucleofector® Guarantee, 2 year ext . . . . . . . . . . . . . . 134
2L-102 Fetal Liver CD133+ 500,000 cells . . . . . . . . . . . . . . . . . . 87 AWM-1001 96-well Shuttle® Guarantee, 1 year ext . . . . . . . . . . . . 134
2L-102A Fetal Liver CD133+ 100,000 cells . . . . . . . . . . . . . . . . . . 87 AWM-1002 96-well Shuttle® Guarantee, 2 year ext . . . . . . . . . . . . 134
2M-101 Bone Marrow CD34+ 100,000 cells . . . . . . . . . . . . . . . . 86 AXA-1001 Nucleofector® 96-well Shuttle® System Rack . . . . . . 133
2M-101A Bone Marrow CD34+ 300,000 cells . . . . . . . . . . . . . . . . 86 BE02-002F MEM Alpha 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
2M-101B Bone Marrow CD34+ 500,000 cells . . . . . . . . . . . . . . . . 86 BE02-007E Trypsin/EDTA (10X) 100 ml . . . . . . . . . . . . . . . . . . . . . . 284
2M-101C Bone Marrow CD34+ 1 million cells . . . . . . . . . . . . . . . . 86 BE02-011F TC-100 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
2M-101D Bone Marrow CD34+ 2 million cells . . . . . . . . . . . . . . . . 86 BE02-012E Gentamicin Sulfate 100 ml . . . . . . . . . . . . . . . . . . 199, 285
2M-102 Bone Marrow CD133+ 500,000 cells . . . . . . . . . . . . . . . 87 BE02-014F Ham’s F10 Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 255
2M-102A Bone Marrow CD133+ 100,000 cells . . . . . . . . . . . . . . . 87 BE02-015BOX Lymphochrome™ 10 × 5 ml . . . . . . . . . . . . . . . . . . . . . . 272
2M-125A Bone Marrow Mono Cells 200 million . . . . . . . . . . . . . . 84 BE02-015E Lymphochrome™ 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 272
2M-125B Bone Marrow Mono Cells >25 million . . . . . . . . . . . . . . 84 BE02-015F Lymphochrome™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . 272
2M-125C Bone Marrow Mono Cells 25 million . . . . . . . . . . . . . . . . 84 BE02-016Q ProCHO™ AT 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267
2M-125D Bone Marrow Mono Cells 100 million . . . . . . . . . . . . . . . 84 BE02-017F PBS EDTA 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
2M-302 Bone Marrow Stromal Cells 5 million cells . . . . . . . . . . 89 BE02-018T PBS-ET 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
2S-101 Quadratox™ 4 Species Panel . . . . . . . . . . . . . . . . . . . . . . . 85 BE02-019F William’s Medium E 500 ml . . . . . . . . . . . . . . . . . . . . . . 259
2S-101A Baboon Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 BE02-020F EMEM with EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
2S-101B Canine Bone Marrow 5 million cells . . . . . . . . . . . . . . . . 85 BE02-026E Amniochrome® Plus 100 ml . . . . . . . . . . . . . . . . . . . . . . 273
2S-101C Murine Bone Marrow 5 million cells . . . . . . . . . . . . . . . . 85 BE02-026F Amniochrome® Plus 500 ml . . . . . . . . . . . . . . . . . . . . . 273
2S-101D Human Bone Marrow 5 million cells . . . . . . . . . . . . . . . 85 BE02-027F Earles BSS with HEPES 500 ml . . . . . . . . . . . . . . . . . . . 282
2T-110 Osteoclast Precusors 1 million cells . . . . . . . . . . . . . . . 92 BE02-028Q ProPer™ 1 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
2W-200 CD4+ Cells 10 million cells . . . . . . . . . . . . . . . . . . . . . . . . 95 BE02-029Q ProDoma™ 1 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 277
2W-400A CD14+ Cells 40 million cells . . . . . . . . . . . . . . . . . . . . . . . 93 BE02-030Q ProVero™ 1 serum-free medium, 1 L . . . . . . . . . . . . . . 270
2W-400B CD14+ Cells 20 million cells . . . . . . . . . . . . . . . . . . . . . . . 93 BE02-032Q ProDoma™ 3 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 277
2W-400C CD14+ Cells 10 million cells . . . . . . . . . . . . . . . . . . . . . . . 93 BE02-035E Amniochrome® Pro 100 ml . . . . . . . . . . . . . . . . . . . . . . . 273
2W-501 Human NK Cells neg selection . . . . . . . . . . . . . . . . . . . . 96 BE02-035F Amniochrome® Pro 500 ml . . . . . . . . . . . . . . . . . . . . . . 273
2W-502 Human NK Cells pos selection . . . . . . . . . . . . . . . . . . . . 96 BE02-036F HTF w/gentamicin 500 ml . . . . . . . . . . . . . . . . . . . . . . . 272
50-647U QCL-1000® 120 tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . 328 BE02-037F HTF HEPES w/gentamicin 500 ml . . . . . . . . . . . . . . . . 272
50-648U QCL-1000® 300 tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . 328 BE02-038P20 Water for Cell Culture 20 L . . . . . . . . . . . . . . . . . . . . . . . 284
50-658U PyroGene®-rFC Kit 192 tests . . . . . . . . . . . . . . . . . . . . . 329 BE02-038P200 Water for Cell Culture 200 L . . . . . . . . . . . . . . . . . . . . . . 284
80-507L Sample Container 25/box . . . . . . . . . . . . . . . . . . . . . . . . 330 BE04-418F X-VIVO™ 15 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
80-507U Sample Container 100/box . . . . . . . . . . . . . . . . . . . . . . . 330 BE04-684E UltraGlutamine II 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 284
AAD-1001 Nucleofector® II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 BE04-687F/U1 DMEM:F12 1:1 w/o HEPES 500ml . . . . . . . . . . . . . . . . 254
AAM-1001 Nucleofector® 96-well Shuttle® . . . . . . . . . . . . . . . . . . . 133 BE04-687Q DMEM:F12 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 254
AC-2509 bMVEC-B Brain MV Endo cryo amp . . . . . . . . . . . . . . . . . .13 BE10-502F Earle’s BSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282
AC-2621A Rat Hepatocytes 6 well plate . . . . . . . . . . . . . . . . . . . . . . .17 BE12-115E RPMI 1640 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-2622A Rat Hepatocytes 12 well plate . . . . . . . . . . . . . . . . . . . . . .17 BE12-115F/U1 RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-2623A Rat Hepatocytes 24 well plate . . . . . . . . . . . . . . . . . . . . . .17 BE12-604F/U1 DMEM 4 .5 g/L glucose, w/ UltraGlutamine 500 ml . 253
AC-2623B Rat Hepatocytes 24 well plate . . . . . . . . . . . . . . . . . . . . . .17 BE12-668F MEM Eagle EBSS (2X) 500 ml . . . . . . . . . . . . . . . . . . . . 257
AC-2624A Rat Hepatocytes 48 well plate . . . . . . . . . . . . . . . . . . . . .17 BE12-702F/U1 RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-2625A Rat Hepatocytes 96 well plate . . . . . . . . . . . . . . . . . . . . .17 BE12-723F UltraDOMA™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 276
AC-2627A Rat Hepatocytes T-25 flask . . . . . . . . . . . . . . . . . . . . . . . .17 BE12-738F Waymouth’s MB 752/1 500 ml . . . . . . . . . . . . . . . . . . . 259
AC-2628A Rat Hepatocytes T-75 flask . . . . . . . . . . . . . . . . . . . . . . . .17 BE12-752F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-2628B Rat Hepatocytes T-75 flask . . . . . . . . . . . . . . . . . . . . . . . .17 BE12-756EZM Amniochrome® II 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 273
AC-2630 Rat Hepatocytes suspension . . . . . . . . . . . . . . . . . . . . . .17 BE12-756FCM Amniochrome® II 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 273
AC-3103 EMVB Brain MV BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . .13 BE12-761F William’s Medium E 500 ml . . . . . . . . . . . . . . . . . . . . . . 259
AC-4207 EMVB Brain MV Endo Medium SingleQuots® . . . . . . . . .13 BE15-029D ProCHO™ 4 powder 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6001T25 bAEC T-25 flask, single donor . . . . . . . . . . . . . . . . . . . . . . 70 BE15-029F ProCHO™ 4 powder 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6001T75 bAEC T-75 flask, single donor . . . . . . . . . . . . . . . . . . . . . . 70 BE15-512D DPBS powder 1 × 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
AC-6001W96 bAEC 96 well plate, single donor . . . . . . . . . . . . . . . . . . . 70 BE15-512F DPBS powder 1 × 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
AC-6002T25 bAEC T-25 flask, pooled . . . . . . . . . . . . . . . . . . . . . . . . . . 70 BE15-604F DMEM (Powder) 4 .5 g/L glucose 50 L . . . . . . . . . . . . . 253
AC-6002T75 bAEC T-75 flask, pooled . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 BE15-702D RPMI 1640 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-6002W96 bAEC 96 well plate, pooled . . . . . . . . . . . . . . . . . . . . . . . . 70 BE15-766D ProCHO™ 5 powder 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6004T25 bPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE15-766F ProCHO™ 5 powder 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6004T75 bPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE15-771D PowerCHO® 2 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268
AC-6004W96 bPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE15-771J PowerCHO® 2 100L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268
Indices
348
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Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
C2501AT150 NHEK-Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2519AW6 HUVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2501AT225 NHEK-Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2519AW96 HUVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2501AT25 NHEK-Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AT150 HUAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AT75 NHEK-Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AT225 HUAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW12 NHEK-Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AT25 HUAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW24 NHEK-Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AT75 HUAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW48 NHEK-Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AW12 HUAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW6 NHEK-Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AW24 HUAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW96 NHEK-Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AW48 HUAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2503A NHEK-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2520AW6 HUAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2503AT150 NHEK-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2520AW96 HUAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2503AT225 NHEK-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AT150 HPAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AT25 NHEK-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AT225 HPAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AT75 NHEK-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AT25 HPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW12 NHEK-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AT75 HPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW24 NHEK-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AW12 HPAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW48 NHEK-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AW24 HPAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW6 NHEK-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AW48 HPAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW96 NHEK-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AW6 HPAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AT150 HMVEC-d Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530AW96 HPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AT225 HMVEC-d Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BT150 HPAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AT25 HMVEC-d Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BT225 HPAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AT75 HMVEC-d Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BT25 HPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW12 HMVEC-d Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BT75 HPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW24 HMVEC-d Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BW12 HPAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW48 HMVEC-d Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BW24 HPAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW6 HMVEC-d Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BW48 HPAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW96 HMVEC-d Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BW6 HPAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507A NHEK-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530BW96 HPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AT150 NHEK-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CT150 HPAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AT225 NHEK-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CT225 HPAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AT25 NHEK-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CT25 HPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AT75 NHEK-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CT75 HPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW12 NHEK-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW12 HPAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW24 NHEK-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW24 HPAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW48 NHEK-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW48 HPAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW6 NHEK-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW6 HPAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW96 NHEK-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW96 HPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2516AT150 HMVEC-d Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AT150 HAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AT225 HMVEC-d Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AT225 HAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AT25 HMVEC-d Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AT25 HAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AT75 HMVEC-d Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AT75 HAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW12 HMVEC-d Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW12 HAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW24 HMVEC-d Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW24 HAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW48 HMVEC-d Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW48 HAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW6 HMVEC-d Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW6 HAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW96 HMVEC-d Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW96 HAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2517A HUVEC Cells, EGM®-2, cryo amp . . . . . . . . . . . . . . . . . . . 33 C2543AT150 HMVEC-d Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AT150 HUVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AT225 HMVEC-d Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AT225 HUVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AT25 HMVEC-d Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AT25 HUVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AT75 HMVEC-d Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AT75 HUVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW12 HMVEC-d Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW12 HUVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW24 HMVEC-d Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW24 HUVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW48 HMVEC-d Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW48 HUVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW6 HMVEC-d Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW6 HUVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW96 HMVEC-d Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW96 HUVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Indices
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32 (0) 87 321 611 www.lonza.com/research
Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
C2585AT150 HCAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0285 NHBE T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
C2585AT225 HCAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0288 HMVEC-d Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 31
C2585AT25 HCAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0291 HIAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
C2585AT75 HCAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0294 SAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
C2585AW12 HCAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0297 NHA T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
C2585AW24 HCAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0310 PrEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
C2585AW48 HCAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0313 UtSMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
C2585AW6 HCAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0322 HUAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2585AW96 HCAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0331 UtMVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
CC-0084 UtMVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2501 NHEK-Ad cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0085 HUAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC2501T150 NHEK-Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0088 PrEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC2501T225 NHEK-Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0089 UtSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2501W12 NHEK-Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0093 NHA 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 CC-2501W24 NHEK-Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0094 SAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2501W48 NHEK-Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0095 HIAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2501W6 NHEK-Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0096 CASMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2503 NHEK-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0100 NHBE 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 CC2503T150 NHEK-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0104 NHEK-Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 CC2503T225 NHEK-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0108 NHEK-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 42 CC-2503W12 NHEK-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0112 HMVEC-d Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 29 CC-2503W24 NHEK-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0116 NHDF-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2503W48 NHEK-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0124 HUVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2503W6 NHEK-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0128 HPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2504 NHEM-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0132 HAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2504T150 NHEM-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0136 NHBE 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2504T225 NHEM-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0140 HMEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2504T25 NHEM-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0144 SkMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2504T75 NHEM-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0148 AoSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 CC-2504W12 NHEM-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0152 PASMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 CC-2504W24 NHEM-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0156 NHEK-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 43 CC-2504W48 NHEM-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0160 NHDF-Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2504W6 NHEM-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0164 NHLF 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2504W96 NHEM-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0168 RPTEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2505 HMVEC-d Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0172 HRCE 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2505T150 HMVEC-d Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0176 NHMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2505T225 HMVEC-d Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0180 BSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2505W12 HMVEC-d Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0184 HMVEC-L 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2505W24 HMVEC-d Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0188 HCAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2505W48 HMVEC-d Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0192 UASMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2505W6 HMVEC-d Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0201 NHEK-Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 CC-2507 NHEK-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0204 NHEK-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 CC2507T150 NHEK-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0207 HMVEC-d Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 CC2507T225 NHEK-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0210 NHDF-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2507W12 NHEK-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0216 HUVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2507W24 NHEK-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0219 HPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2507W48 NHEK-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0222 HAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2507W6 NHEK-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0225 NHBE T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2508 PrSC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0228 HMEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC2508T150 PrSC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0231 SkMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC2508T225 PrSC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0234 AoSMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 CC-2508T75 PrSC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0237 PASMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 CC-2508W12 PrSC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0240 BSMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2508W24 PrSC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0243 UASMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2508W48 PrSC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0246 HMVEC-d Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29 CC-2508W6 PrSC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0252 NHDF-Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2508W96 PrSC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0255 NHEK-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Indices
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Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
CC2511T225 NHDF-Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2533W96 BdSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65
CC-2511W12 NHDF-Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2535 HAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2511W24 NHDF-Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC2535T150 HAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2511W48 NHDF-Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC2535T225 HAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2511W6 NHDF-Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2535W12 HAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2512 NHLF cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2535W24 HAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC2512T150 NHLF T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2535W48 HAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC2512T225 NHLF T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2535W6 HAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2512W12 NHLF 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2538 NHOst cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2512W24 NHLF 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC2538T150 NHOst T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2512W48 NHLF 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC2538T225 NHOst T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2512W6 NHLF 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2538T25 NHOst T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516 HMVEC-d Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538T75 NHOst T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC2516T150 HMVEC-d Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W12 NHOst 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC2516T225 HMVEC-d Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W24 NHOst 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516W12 HMVEC-d Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W48 NHOst 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516W24 HMVEC-d Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W6 NHOst 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516W48 HMVEC-d Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W96 NHOst 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516W6 HMVEC-d Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2540 NHBE cryo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2516W96 HMVEC-d Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 31 CC2540T150 NHBE T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2517 HUVEC Cells, EGM®, cryo amp . . . . . . . . . . . . . . . . . . . . . 33 CC2540T225 NHBE T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC2517T150 HUVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2540W12 NHBE 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC2517T225 HUVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2540W24 NHBE 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2517W12 HUVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2540W48 NHBE 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2517W24 HUVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2540W6 NHBE 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2517W48 HUVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2541 NHBE cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2517W6 HUVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC2541T150 NHBE T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2519 HUVEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC2541T225 NHBE T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC2519T150 HUVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2541W12 NHBE 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC2519T225 HUVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2541W24 NHBE 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2519W12 HUVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2541W48 NHBE 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2519W24 HUVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2541W6 NHBE 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2519W48 HUVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2543 HMVEC-d Ad cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2519W6 HUVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC2543T150 HMVEC-d Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2519W96 HUVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC2543T225 HMVEC-d Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2520 HUAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W12 HMVEC-d Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC2520T150 HUAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W24 HMVEC-d Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC2520T225 HUAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W48 HMVEC-d Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2520W12 HUAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W6 HMVEC-d Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2520W24 HUAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W96 HMVEC-d Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2520W48 HUAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2545 HIAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2520W6 HUAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC2545T150 HIAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2527 HMVEC-L cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC2545T225 HIAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC2527T150 HMVEC-L T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2545W12 HIAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC2527T225 HMVEC-L T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2545W24 HIAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2527W12 HMVEC-L 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2545W48 HIAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2527W24 HMVEC-L 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2545W6 HIAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2527W48 HMVEC-L 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2547 SAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2527W6 HMVEC-L 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC2547T150 SAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2530 HPAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC2547T225 SAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC2530T150 HPAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2547W12 SAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC2530T225 HPAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2547W24 SAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2530W12 HPAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2547W48 SAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2530W24 HPAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2547W6 SAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2530W48 HPAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2550 NHAC-kn cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2530W6 HPAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC2550T150 NHAC-kn T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2533 BdSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65
Indices
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Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
CC2551T150 HMEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2565 NHA Cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC2551T225 HMEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC2565T150 NHA T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2551W12 HMEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC2565T225 NHA T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2551W24 HMEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2565W12 NHA 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2551W48 HMEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2565W24 NHA 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2551W6 HMEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2565W48 NHA 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2553 RPTEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2565W6 NHA 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC2553T150 RPTEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2571 AoSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC2553T225 RPTEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2571T150 AoSMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2553W12 RPTEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2571T225 AoSMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2553W24 RPTEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2571W12 AoSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2553W48 RPTEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2571W24 AoSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2553W6 RPTEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2571W48 AoSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2554 HRCE cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2571W6 AoSMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC2554T150 HRCE T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2576 BSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC2554T225 HRCE T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2576T150 BSMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2554W12 HRCE 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2576T225 BSMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2554W24 HRCE 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2576W12 BSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2554W48 HRCE 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2576W24 BSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2554W6 HRCE 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2576W48 BSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2555 PrEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2576W6 BSMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC2555T150 PrEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2579 UASMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC2555T225 PrEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC2579T150 UASMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2555W12 PrEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC2579T225 UASMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2555W24 PrEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2579W12 UASMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2555W48 PrEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2579W24 UASMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2555W6 PrEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2579W48 UASMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2556 HRE cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2579W6 UASMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC2556T150 HRE T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580 HSMM cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC2556T225 HRE T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2580T150 NHAC-kn T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556T25 HRE T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2580T225 NHAC-kn T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556T75 HRE T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580T25 HSMM T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W12 HRE 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580T75 HSMM T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W24 HRE 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580W12 HSMM 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W48 HRE 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580W24 HSMM 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W6 HRE 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580W48 HSMM 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W96 HRE 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580W6 HSMM 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2559 NHMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2580W96 HSMM 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC2559T150 NHMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2581 PASMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC2559T225 NHMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2581T150 PASMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2559W12 NHMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2581T225 PASMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2559W24 NHMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2581W12 PASMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2559W48 NHMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2581W24 PASMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2559W6 NHMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2581W48 PASMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2561 SkMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2581W6 PASMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC2561T150 SkMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2583 CASMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC2561T225 SkMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC2583T150 CASMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2561W12 SkMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC2583T225 CASMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2561W24 SkMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2583W12 CASMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2561W48 SkMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2583W24 CASMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2561W6 SkMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2583W48 CASMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2562 UtSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2583W6 CASMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC2562T150 UtSMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2585 HCAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC2562T225 UtSMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC2585T150 HCAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2562W12 UtSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC2585T225 HCAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2562W24 UtSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2585W12 HCAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2562W48 UtSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Indices
352
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Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
CC-2587W12 PrSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810 HMVEC-dLyAd cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2587W24 PrSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810T150 HMVEC-dLyAd T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2587W48 PrSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810T225 HMVEC-dLyAd T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2587W6 PrSMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810T25 HMVEC-dLyAd T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2587W96 PrSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810T75 HMVEC-dLyAd T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2591 h NHEPS® cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 CC-2810W12 HMVEC-dLyAd 12 well plate . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2601 NHEK-Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 CC-2810W24 HMVEC-dLyAd 24 well plate . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2603 NHEK-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 CC-2810W48 HMVEC-dLyAd 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2605 HMVEC-d Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29 CC-2810W6 HMVEC-dLyAd 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2607 NHEK-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 CC-2810W96 HMVEC-dLyAd 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2608 PrSC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2811 HMVEC-dBlAd cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2609 NHDF-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2811T150 HMVEC-dBlAd T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2611 NHDF-Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2811T225 HMVEC-dBlAd T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2612 NHLF T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2811T25 HMVEC-dBlAd T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2616 HMVEC-d Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2811T75 HMVEC-dBlAd T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2617 HUVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2811W12 HMVEC-dBlAd 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2619 HUVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2811W24 HMVEC-dBlAd 24 well plate . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2620 HUAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2811W48 HMVEC-dBlAd 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2627 HMVEC-L T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2811W6 HMVEC-dBlAd 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2630 HPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2811W96 HMVEC-dBlAd 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2635 HAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2812 HMVEC-dLyNeo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2640 NHBE T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2812T150 HMVEC-dLyNeo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2641 NHBE T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 CC-2812T225 HMVEC-dLyNeo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2643 HMVEC-d Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 CC-2812T25 HMVEC-dLyNeo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2644 UtMVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2812T75 HMVEC-dLyNeo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2645 HIAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2812W12 HMVEC-dLyNeo 12 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2647 SAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2812W24 HMVEC-dLyNeo 24 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2651 HMEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2812W48 HMVEC-dLyNeo 48 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2653 RPTEC T-25 Flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2812W6 HMVEC-dLyNeo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2654 HRCE T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2812W96 HMVEC-dLyNeo 96 well plate . . . . . . . . . . . . . . . . . . . . . 30
CC-2655 PrEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2813 HMVEC-dBlNeo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2659 NHMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2813T150 HMVEC-dBlNeo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2661 SkMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2813T225 HMVEC-dBlNeo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2662 UtSMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2813T25 HMVEC-dBlNeo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2665 NHA T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 CC-2813T75 HMVEC-dBlNeo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2671 AoSMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 CC-2813W12 HMVEC-dBlNeo 12 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2676 BSMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2813W24 HMVEC-dBlNeo 24 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2679 UASMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2813W48 HMVEC-dBlNeo 48 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2681 PASMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 CC-2813W6 HMVEC-dBlNeo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2683 CASMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2813W96 HMVEC-dBlNeo 96 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2685 HCAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2814 HMVEC-Lly cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2690 h NHEPS® suspension . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 CC-2814T150 HMVEC-Lly T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2691A h NHEPS® collagen, 6 well plate . . . . . . . . . . . . . . . . . . . .15 CC-2814T225 HMVEC-Lly T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2691B h NHEPS® Matrigel®, 6 well plate . . . . . . . . . . . . . . . . . . .15 CC-2814T25 HMVEC-Lly T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2692A h NHEPS® collagen, 12 well plate . . . . . . . . . . . . . . . . . . .15 CC-2814T75 HMVEC-Lly T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2692B h NHEPS® Matrigel®, 12 well plate . . . . . . . . . . . . . . . . . .15 CC-2814W12 HMVEC-Lly 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2693A h NHEPS® collagen, 24 well plate . . . . . . . . . . . . . . . . . . .15 CC-2814W24 HMVEC-Lly 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2693B h NHEPS® Matrigel®, 24 well plate . . . . . . . . . . . . . . . . . .15 CC-2814W48 HMVEC-Lly 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2694 h NHEPS® collagen, T-25 flask . . . . . . . . . . . . . . . . . . . . . .15 CC-2814W6 HMVEC-Lly 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2694B h NHEPS® Matrigel®, T-25 flask . . . . . . . . . . . . . . . . . . . . .15 CC-2814W96 HMVEC-Lly 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2695 h NHEPS® collagen, T-75 flask . . . . . . . . . . . . . . . . . . . . . .15 CC-2815 HMVEC-LBl cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2695B h NHEPS® Matrigel®, T-75 flask . . . . . . . . . . . . . . . . . . . . .15 CC-2815T150 HMVEC-LBl T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2696 h NHEPS® collagen, T-150 flask . . . . . . . . . . . . . . . . . . . . .15 CC-2815T225 HMVEC-LBl T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2696B h NHEPS® Matrigel®, T-150 flask . . . . . . . . . . . . . . . . . . . .15 CC-2815T25 HMVEC-LBl T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2698A h NHEPS® collagen,96 well plate . . . . . . . . . . . . . . . . . . .15 CC-2815T75 HMVEC-LBl T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2698B h NHEPS® Matrigel®, 96 well plate . . . . . . . . . . . . . . . . . .15 CC-2815W12 HMVEC-LBl 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2701 NHDC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Indices
353
32 (0) 87 321 611 www.lonza.com/research
Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
CC-3104 KBM® Basal Medium w/o Ca++ 500 ml . . . . . . . . . . . . . . 44 CC-3275 RCBM Basal Medium, 200ml . . . . . . . . . . . . . . . . . . . . . . .72
CC-3107 KGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 -44 CC-4009 Bovine Pit . Extract (BPE) 2 ml . . . . . . . . . . . . . . . . . . . . 78
CC-3108 KGM®-2 BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . 44 CC-4068 hFGF 1 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3111 KGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . .41, 42, 43, 44 CC-4098 Bovine Brain Ext . (BBE) 5 ml . . . . . . . . . . . . . . . . . . . . . . 78
CC-3112 KGM® BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . 44 CC-4107 hEGF 3 µg/mi 0 .5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3118 SAGM™ BulletKit® (CC-3119 and CC-4124) . . . . . . . 19, 20 CC-4123 AGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 50, 77
CC-3119 SABM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 20 CC-4124 SAEC SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-3121 EBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 35 CC-4126 FGM®-2 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 39
CC-3124 EGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . 33, 34, 35 CC-4127 REGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . 11, 57
CC-3125 EGM®-MV BulletKit® . . . . . . . . . . . . . . . . . . . . 33, 35, 70, 71 CC-4131 KGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
CC-3129 EBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 35 CC-4133 EGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
CC-3130 FGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-4134 FGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
CC-3131 FBM® Fibroblast Basal 500 ml . . . . . . . . . . . . . . . . . . . . . 39 CC-4136 MEGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 48
CC-3132 FGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38, 39 CC-4139 SkGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 63
CC-3146 MsGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56, 57 CC-4143 EGM®-MV SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 35
CC-3147 MsBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 57 CC-4146 MsGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 57
CC-3150 MEGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-4147 EGM®-2 MV SingleQuots® Kit . . . . . . . . . . . . . . . . . . . 22, 35
CC-3151 MEBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 48 CC-4149 SmGM®-2 SingleQuots® Kit . . . . . . . . . . . . . .22, 53, 65, 68
CC-3152 MEBM® Basal CC-4152 KGM®-2 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 44
Sodium Bicarbinate-free Medium 500 ml . . . . . . . . . 48 CC-4175 BEGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-3153 MEBM® Basal Phenol Red-free Medium 500 ml . . . . . 48 CC-4176 EGM®-2 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 35
CC-3156 EBM®-2 Basal Medium 500 ml . . . . . . . . . . . . . 13, 22, 35 CC-4177 PrEGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . 53, 67
CC-3158 KBM®-2 Basal Medium w/o Ca++ 500 ml . . . . . . . . . . . . 44 CC-4181 SCGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . 39, 53
CC-3160 SkGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 - 63 CC-4182 HCM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 15, 17
CC-3161 SkBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 63 CC-4192 HMM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 15, 17
CC-3162 EGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . 25, 32, 33, 34, 35 CC-4193 OGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
CC-3165 PrEBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . 53, 67 CC-4194 OGM™ Diff . SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . 60
CC-3166 PrEGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . 52, 53, 67 CC-4202 Calcium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3170 BEGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19, 20 CC-4205 Human Transferrin 0 .5 ml . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3171 BEBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 20 CC-4241 Neural Prog . Maint . Med . . . . . . . . . . . . . . . . . . . . . . . . . . 97
CC-3181 SmBM® Basal Medium 500 ml . . . . . . . . . .22, 53, 65, 68 CC-4242 Neural Progenitor Suppl . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
CC-3182 SmGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . 22, 53, 65 - 68 CC-4323 NSF-1 4 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3186 AGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . 50, 75 - 77 CC-4398 Ascorbic Acid 0 .5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 60, 78
CC-3187 ABM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . 50, 77 CC-4408 CDM SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
CC-3190 REGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . 11, 55 - 57 CC-4409 CGM SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
CC-3191 REBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . 11, 57 CC-4435 MGM®-4 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-3197 HMM™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15, 17 CC-4444 Corneal Model BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
CC-3198 HCM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15, 17 CC-4455 KGM®-CD BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
CC-3199 HBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . 15, 17 CC-4456 KGM®-CD SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 44
CC-3202 EGM®-2 MV BulletKit® . . . . . . . . . . . . . .22, 25 - 31, 34, 35 CC-4461 PNGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . .74, 76, 77
CC-3204 SCBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . 39, 53 CC-4462 PNGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 77
CC-3205 SCGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . 37, 39, 52, 53 CC-4510 Endothelin-3 (ET-3), 130 µg . . . . . . . . . . . . . . . . . . . . . . . 46
CC-3207 OGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59, 60 CC-4511 PNGM™-A SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 77
CC-3208 OBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 60 CC-4512 PNGM™-A BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . 74, 77
CC-3209 NPMM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 CC-4515 RCGM BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72
CC-3210 NPBM™ Medium 200 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 CC-4516 RCGM SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . .72
CC-3211 LGM-3® Growth Medium 500 ml . . . . . . . . . . . . . . . . . . . 94 CC-5002 Trypsin Neutralizing Soln 100 ml . . . . . . . . . . . . . . . 46, 78
CC-3216 CGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 - 60 CC-5012 Trypsin/EDTA Solution 100 ml . . . . . . . . . . . . . . . . . . 46, 78
CC-3217 CBM Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . . 60 CC-5022 HEPES Buffered Saline 100 ml . . . . . . . . . . . . . . . . . . . . 78
CC-3225 CDM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 - 60 CC-5024 HEPES Buffered Saline 500 ml . . . . . . . . . . . . . . . . . . . . 78
CC-3226 CDM™ Basal Medium 250 ml . . . . . . . . . . . . . . . . . . . . . . . 60 CC-5034 ReagentPack™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
CC-3229 NPDM BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 . . . . 20, 22, 35, 39, 44, 48, 50, 53, 57, 60, 63, 68, 77, 78
CC-3232 Trypsin/EDTA for MSC 100 ml . . . . . . . . . . . . . . . . . . . . . . 90 CC-7014 AoAF cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3233 Chondrocyte ReagentPack™ . . . . . . . . . . . . . . . . . . . . . . . 60 CC7014T150 AoAF T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3234 Sodium Alginate 50 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 CC7014T225 AoAF T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3235 Calcium Chloride Solution 500 ml . . . . . . . . . . . . . . . . . 60 CC-7014T25 AoAF T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3236 Sodium Chloride Solution 500 ml . . . . . . . . . . . . . . . . . . 60 CC-7014T75 AoAF T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Indices
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Cat. No. Description Page Number Cat. No. Description Page Number
CC-7016T25 HMVEC-Bd T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-418 MycoAlert® Kit 50 Tests . . . . . . . . . . . . . . . . . . . . . . . . . 220
CC-7016T75 HMVEC-Bd T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-500 PKLight® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230
CC-7016W12 HMVEC-Bd 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-501 PKLight® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230
CC-7016W24 HMVEC-Bd 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-517 ToxiLight® Control 10 ml . . . . . . . . . . . . . . . . . . . . . . . . . 226
CC-7016W48 HMVEC-Bd 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-518 MycoAlert® Control Set 10 Tests . . . . . . . . . . . . . . . . . . 220
CC-7016W6 HMVEC-Bd 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-600 PDELight® Kit 500 tests . . . . . . . . . . . . . . . . . . . . . . . . . 232
CC-7016W96 HMVEC-Bd 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-610 PPiLight™ Assay 500 tests . . . . . . . . . . . . . . . . . . . . . . . 234
CC-7030 HMVEC-C cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT07-818 MycoZap™ 1 Treatment . . . . . . . . . . . . . . . . . . . . . 198, 222
CC-7030T150 HMVEC-C T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT07-918 MycoZap™ 5 Treatments . . . . . . . . . . . . . . . . . . . . 198, 222
CC-7030T225 HMVEC-C T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT17-217 ToxiLight® Assay Kit with, 500 tests . . . . . . . . . . . . . . 226
CC-7030T25 HMVEC-C T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT17-221 ViaLight® Plus 500 tests . . . . . . . . . . . . . . . . . . . . . . . . . 223
CC-7030T75 HMVEC-C T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT27-008 ATP Standard 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241
CC-7030W12 HMVEC-C 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT27-040 ExPro™ Cleaning Solution 50 ml . . . . . . . . . . . . . . . . . . 241
CC-7030W24 HMVEC-C 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT27-102 Tissue Culture Plate, White . . . . . . . . . . . . . . . . . . . . . . . 241
CC-7030W48 HMVEC-C 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 M-AsM-330 Mouse C57 Mixed cryo amp . . . . . . . . . . . . . . . . . . . . . . . 76
CC-7030W6 HMVEC-C 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 M-AsM-430 Mouse CD1 Mixed Astrocyte cryo amp . . . . . . . . . . . . . 76
CC-7030W96 HMVEC-C 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 M-Cp-302 Mouse C57 Striatum Neuron cryo amp . . . . . . . . . . . . . 76
CC-7049 HPdLF cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 M-Cp-402 Mouse CD1 Striatum Neuron cryo amp . . . . . . . . . . . . . 76
CC-7049T150 HPdLF T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 M-Cx-300 Mouse C57 Cortex Neurons cryo amp . . . . . . . . . . . . . . 76
CC-7049T225 HPdLF T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 M-Cx-400 Mouse CD1 Cortex Neurons cryo amp . . . . . . . . . . . . . . 76
CC-7049T25 HPdLF T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N183-06 PYROGENT® 80 test kit 0 .06 . . . . . . . . . . . . . . . . . . . . . 326
CC-7049T75 HPdLF T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N183-125 PYROGENT® 80 test kit 0 .125 . . . . . . . . . . . . . . . . . . . . . 326
CC-7049W12 HPdLF 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N184-25 PYROGENT® 250 test kit 0 .25 . . . . . . . . . . . . . . . . . . . . 326
CC-7049W24 HPdLF 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N185 Endotoxin (E.coli 055:B5) . . . . . . . . . . . . . . . . . . . . . . . 327
CC-7049W48 HPdLF 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N186 Endotoxin (E.coli 055:B5) . . . . . . . . . . . . . . . . . . . . . . . 327
CC-7049W6 HPdLF 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N188 Pyrosperse® Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
CC-7049W96 HPdLF 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N189-06 PYROGENT®-25 SingleTest Vial 0 .06 . . . . . . . . . . . . . . 326
CMS-2000 Renal Epithelial Cell Model . . . . . . . . . . . . . . . . . . . . . . . . 11 N189-125 PYROGENT®-25 SingleTest Vial 0 .125 . . . . . . . . . . . . . . 326
CMS-2015 Corneal Epithelial Model . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 N189-25 PYROGENT®-25 SingleTest Vial 0 .25 . . . . . . . . . . . . . . . 326
DE14-417E Calf Serum Newborn 100 ml . . . . . . . . . . . . . . . . . . . . . 295 N190 b-G-Blocker™ 5 × 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
DE14-417F Calf Serum Newborn 500 ml . . . . . . . . . . . . . . . . . . . . . 295 N194-03 PYROGENT® 250 test kit 0 .03 . . . . . . . . . . . . . . . . . . . . 326
DE14-701E FBS USDA 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 297 N194-06 PYROGENT® 250 test kit 0 .06 . . . . . . . . . . . . . . . . . . . . 326
DE14-701F FBS USDA 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 297 N194-125 PYROGENT® 250 test kit 0 .125 . . . . . . . . . . . . . . . . . . . 326
DE14-801E FBS EU Standard 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 297 N201 Pyrogen-free Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . 330
DE14-801F FBS EU Standard 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 297 N205 Pyrogen-free Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . 330
DE14-802E FBS EU Standard, Research Grade, 100 ml . . . . . . . . . 297 N207 Pyrogen-free Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . 330
DE14-802F FBS EU Standard, Research Grade, 500 ml . . . . . . . . 297 N283-06 PYROGENT® Plus 64 tests with CSE 0 .06 . . . . . . . . . . 326
DE14-810E FBS EU Standard (Dialyzed) 100 ml . . . . . . . . . . . . . . 297 N283-125 PYROGENT® Plus 64 tests 0 .125 . . . . . . . . . . . . . . . . . . 326
DE14-810F FBS EU Standard (Dialyzed) 500 ml . . . . . . . . . . . . . . 297 N284-25 PYROGENT® Plus 200 tests 0 .25 . . . . . . . . . . . . . . . . . . 326
DE14-820E FBS EU Standard (charcoal) 100 ml . . . . . . . . . . . . . . 297 N289-06 PYROGENT® Plus single-test with CSE 0 .06 . . . . . . . . 326
DE14-820F FBS EU Standard (charcoal) 500 ml . . . . . . . . . . . . . . 297 N289-125 PYROGENT® Plus single-test with CSE 0 .125 . . . . . . . 326
DE14-830E FBS EU Std (tetracycline free) 100 ml . . . . . . . . . . . . 297 N289-25 PYROGENT® Plus single-test with CSE 0 .25 . . . . . . . . 326
DE14-830F FBS EU Std (tetracycline free) 500 ml . . . . . . . . . . . . 297 N294-03 PYROGENT® Plus 200 tests with CSE 0 .03 . . . . . . . . . 326
DE14-840E FBS EU Standard 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 296 N294-06 PYROGENT® Plus 200 tests with CSE 0 .06 . . . . . . . . . 326
DE14-840F FBS EU Standard 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 296 N294-125 PYROGENT® Plus 200 tests 0 .125 . . . . . . . . . . . . . . . . . 326
DE14-850E FBS EU Standard ES 100 ml . . . . . . . . . . . . . . . . . . . . . . 296 N594-03 PYROGENT® Ultra Gel Clot 0 .03 . . . . . . . . . . . . . . . . . . . 327
DE14-850F FBS EU Standard ES 500 ml . . . . . . . . . . . . . . . . . . . . . . 296 N594-06 PYROGENT® Ultra Gel Clot 0 .06 . . . . . . . . . . . . . . . . . . . 327
DE14-860F FBS EU Standard Ultralow IgG 500 ml . . . . . . . . . . . . . 297 N594-125 PYROGENT® Ultra Gel Clot 0 .125 . . . . . . . . . . . . . . . . . . 327
DE14-870E FBS, EU Standard, low IgG 100 ml . . . . . . . . . . . . . . . . . 296 PA-1000 OsteoAssay™ Bone Plate . . . . . . . . . . . . . . . . . . . . . . . . . 237
DE14-870F FBS, EU Standard, low IgG 500 ml . . . . . . . . . . . . . . . . . 296 PA-1500 OsteoLyse™ Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 238
E50-640 Endotoxin for QCL-1000® . . . . . . . . . . . . . . . . . . . . . . . . . 328 PA-1503 OsteoImage™ Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 240
LT07-111 ViaLight® HS 1,000 tests . . . . . . . . . . . . . . . . . . . . . . . . 223 PA-3010 Phalloidin AlexaFluor®150 U . . . . . . . . . . . . . . . . . . . . . 242
LT07-115 ApoGlow® Screening Kit . . . . . . . . . . . . . . . . . . . . . . . . . 228 PA-3011 Cell Tracker™ Green 250 µg . . . . . . . . . . . . . . . . . . . . . . . 242
LT07-117 ToxiLight® Assay Kit 1,000 tests . . . . . . . . . . . . . . . . . 226 PA-3012 Cell Tracker™ Orange 250 µg . . . . . . . . . . . . . . . . . . . . . . 242
LT07-118 MycoAlert® Kit 10 Tests . . . . . . . . . . . . . . . . . . . . . . . . . . 220 PA-3013 DAPI 10 mg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 243
LT07-121 ViaLight® Plus 1,000 tests . . . . . . . . . . . . . . . . . . . . . . . 223 PA-3014 Hoechst 33342 Dye 10 ml . . . . . . . . . . . . . . . . . . . . . . . 243
LT07-122 ViaLight® Plus MDA 1,000 tests . . . . . . . . . . . . . . . . . . 223
Indices
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Cat. No. Description Page Number Cat. No. Description Page Number
PA-3269 PrimeFect™ siRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 PX-2591RL h NHEPS® Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
PA-3271 PrimeFect™ Diluent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 PX-2599RL NHNP Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
PA-4490 CalciFluor™ Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 239 R-AsM-530 Rat Brain Cx-Hi-Cp Mix cryo amp . . . . . . . . . . . . . . . . . . . 75
PA-6001RL bAEC Cell Pellet, single donor . . . . . . . . . . . . . . . . . . . . . . 70 R-Cb-503 Rat Cerebellar Neurons cyro amp . . . . . . . . . . . . . . . . . . .74
PA-6002RL bAEC Cell Pellet, pooled . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 R-CM-561 Rat Cardiac Myocytes cryo amp . . . . . . . . . . . . . . . . . . . .72
PA-6004RL bPAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 R-Cp-502 Rat Brain Striatum Neuron cryo amp . . . . . . . . . . . . . . . .74
PA-6005RL bCAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 R-CpAs-522 Rat Brain Striatum Astro cryo amp . . . . . . . . . . . . . . . . 75
PT-2501 Mesenchymal Stem Cells cryo amp . . . . . . . . . . . . . . . . 90 R-Cx-500 Rat Brain Cortex Neurons cryo amp . . . . . . . . . . . . . . . . .74
PT-2599 NHMP cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 R-CxAs-520 Rat Brain Cortex Astro . cryo amp . . . . . . . . . . . . . . . . . . 75
PT-3001 MSCBM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90 R-Drg-505 Rat Dorsal Root Ganglion cryo amp . . . . . . . . . . . . . . . . .74
PT-3002 hMSC Osteogenic BulletKit® . . . . . . . . . . . . . . . . . . . . . . . 90 R-Hi-501 Rat Brain HC Neurons cryo amp . . . . . . . . . . . . . . . . . . . .74
PT-3003 hMSC Chondro BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . 90 R-HiAs-521 Rat Brain HC Astrocytes cryo amp . . . . . . . . . . . . . . . . . 75
PT-3004 hMSC Adipogenic BulletKit® . . . . . . . . . . . . . . . . . . . . . . . 90 S50-641 10 mM MgCl2 Solution 10 ml . . . . . . . . . . . . . . . . . . . . . 330
PT-3238 MSCBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . 90 S50-642 50 mM Tris Buffer 30 ml . . . . . . . . . . . . . . . . . . . . . . . . . 330
PT-3926 HPGM™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 SBA-1001 96-well Shuttle® Automation Package . . . . . . . . . . . . 135
PT-4105 MSCGM™ hMSC SingleQuots® Kit . . . . . . . . . . . . . . . . . . . 90 T100A Retronectin® Rec 0 .5 mg . . . . . . . . . . . . . . . . . . . . .78, 204
PT-4124 rhTGF-b3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90 T100B Retronectin® Rec 2 .5 mg . . . . . . . . . . . . . . . . . . . . .78, 204
PT-5001 Pre-adipocytes, sub cutan . . . . . . . . . . . . . . . . . . . . . . . . 91 T110A Retronectin® Rec 35 mm dishes . . . . . . . . . . . . . .78, 204
PT-5005 Pre-adipocytes, visceral . . . . . . . . . . . . . . . . . . . . . . . . . . 91 VBA-1001 HiFect™ Kit, 1 x 825 µl . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
PT-5020 Pre-adipocytes, sub cutan . . . . . . . . . . . . . . . . . . . . . . . . 91 VBA-4001 HiFect™ Kit, 4 x 825 µl . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
PT-7009 AdipoRed™ Assay Reagent . . . . . . . . . . . . . . . . . . . .91, 236 VCA-1001 Cell Line Nucleofector® Kit R . . . . . . . . . . . . . . . . . . . . . 185
PT-8001 OCP BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92 VCA-1002 Cell Line Nucleofector® Kit T . . . . . . . . . . . . . . . . . . . . . . 185
PT-8002 PGM™-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 VCA-1003 Cell Line Nucleofector® Kit V . . . . . . . . . . . . . . . . . . . . . 185
PT-8201 OCP Osteoclast Precur . Basal Medium 100 ml . . . . . . 92 VCA-1004 Cell Line Nucleofector® Kit C . . . . . . . . . . . . . . . . . . . . . 185
PT-8202 PGM™-2 Basal Medium, 500 ml . . . . . . . . . . . . . . . . . . . . 91 VCA-1005 Cell Line Nucleofector® Kit L . . . . . . . . . . . . . . . . . . . . . 185
PT-9501 OCP Osteoclast Precur . SingleQuots® Kit . . . . . . . . . . . 92 VCO-1001 Cell Line Optimization . . . . . . . . . . . . . . . . . . . . . . . . . . . 186
PT-9502 PGM™-2 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 91 Nucleofector® Kit, 18 reactions
PX-2501RL NHEK-Ad Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 VDC-1040 pmax Cloning™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 197
PX-2503RL NHEK-Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 VDF-1011 pmaxFPTM-Green-C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194
PX-2505RL HMVEC-d Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . 29 VDF-1012 pmaxFPTM-Green-N . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194
PX-2507RL NHEK-Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 VDF-1013 pmaxFPTM-Green-PRL, . . . . . . . . . . . . . . . . . . . . . . . . . . 194
PX-2508RL PrSC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 VDF-1021 pmaxFPTM-Yellow-C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
PX-2509RL NHDF-Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 VDF-1022 pmaxFPTM-Yellow-N . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
PX-2511RL NHDF-Ad Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 VDF-1023 pmaxFPTM-Yellow-PRL . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
PX-2512RL NHLF Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 VDF-1031 pmaxFPTM-Red-C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
PX-2516RL HMVEC-d Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . 31 VDF-1032 pmaxFPTM-Red-N . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
PX-2517RL HUVEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 VDF-1033 pmaxFPTM-Red-PRL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
PX-2519RL HUVEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 VFP-1001 Peptide Transfection Control . . . . . . . . . . . . . . . . . . . . . 201
PX-2520RL HUAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 VGA-1001 cGMPCell Line Nucleofector® Kit R . . . . . . . . . . . . . . . . 188
PX-2527RL HMVEC-L Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 VGA-1002 cGMPCell Line Nucleofector® Kit T . . . . . . . . . . . . . . . . 188
PX-2530RL HPAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 VGA-1003 cGMPCell Line Nucleofector® Kit V . . . . . . . . . . . . . . . . 188
PX-2535RL HAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 VGA-1004 cGMPCell Line Nucleofector® Kit C . . . . . . . . . . . . . . . . 188
PX-2538RL NHOst Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 VGA-1005 cGMPCell Line Nucleofector® Kit L . . . . . . . . . . . . . . . . 188
PX-2540RL NHBE Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 VHCA-1001 Cell Line 96-well Nucleofector® Kit SE, 96 rxns . . . . 185
PX-2541RL NHBE Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 VHCA-1002 Cell Line 96-well Nucleofector® Kit SF, 96 rxns . . . . . 185
PX-2543RL HMVEC-d Ad Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 VHCA-1003 Cell Line 96-well Nucleofector® Kit SG, 96 rxns . . . . 185
PX-2545RL HIAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 VHCA-2001 Cell Line 96-well Nucleofector® Kit SE, 960 rxns . . . 185
PX-2547RL SAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 VHCA-2002 Cell Line 96-well Nucleofector® Kit SF, 960 rxns . . . . 185
PX-2550RL NHAC-kn Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 VHCA-2003 Cell Line 96-well Nucleofector® Kit SG, 960 rxns . . . . 185
PX-2551RL HMEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 VHCA-4001 HeLaS3 96-well Automation Nucleofector® Kit . . . . . 135
PX-2553RL RPTEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 VHCA-4003 Neuro2a 96-well Automation Nucleofector® Kit . . . . 135
PX-2554RL HRCE Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 VHCA-4004 HeLa 96-well Automation Nucleofector® Kit . . . . . . . 135
PX-2555RL PrEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 VHCO-1001 Cell Line Optimization 96-well . . . . . . . . . . . . . . . . . . . 187
PX-2556RL HRE Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Nucleofector® Kit, 96 reactions
PX-2559RL NHMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 VHPA-1001 Human B Cell 96 reactions . . . . . . . . . . . . . . . . . . . . . . . 140
PX-2561RL SkMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 VHPA-1002 Human T Cell 96 reactions . . . . . . . . . . . . . . . . . . . . . . . 149
PX-2562RL UtSMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Indices
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Numerical Index
Continued
Cat. No. Description Page Number Cat. No. Description Page Number
VHPA-2007 Human Monocyte 960 reactions . . . . . . . . . . . . . . . . . 147 VPA-1003 Human CD34+ 25 reactions . . . . . . . . . . . . . . . . . .142, 176
VHPA-2008 Human Macrophage 960 reactions . . . . . . . . . . . . . . . 144 VPA-1004 Human Dendritic 25 reactions . . . . . . . . . . . . . . . . . . . 142
VHPA-2010 Mouse B Cell 960 reactions . . . . . . . . . . . . . . . . . . . . . . 141 VPA-1005 Human NK Cell 25 reactions . . . . . . . . . . . . . . . . . . . . . 148
VHPA-2011 Mouse Dendritic Cell (Imm .) 960 reactions . . . . . . . 143 VPA-1006 Mouse T Cell 25 reactions . . . . . . . . . . . . . . . . . . . . . . . 150
VHPA-2012 Mouse Dendritic Cell (Mat .) 960 reactions . . . . . . . . 143 VPA-1007 Human Monocyte 25 reactions . . . . . . . . . . . . . . . . . . . 146
VHPB-1002 HUVEC 96-well 96 reactions . . . . . . . . . . . . . . . . . . . . . 157 VPA-1008 Human Macrophage 25 reactions . . . . . . . . . . . . . . . . 144
VHPB-2002 HUVEC 96-well 960 reactions . . . . . . . . . . . . . . . . . . . . 157 VPA-1009 Mouse Macrophage 25 reactions . . . . . . . . . . . . . . . . . 145
VHPD-1001 Human Dermal Fibroblast 96 reactions . . . . . . . . . . . 151 VPA-1010 Mouse B Cell 25 reactions . . . . . . . . . . . . . . . . . . . . . . . 141
VHPD-2001 Human Dermal Fibroblast 960 reactions . . . . . . . . . . 151 VPA-1011 Mouse Dendritic (Imm .), 25 reactions . . . . . . . . . . . . 143
VHPF-1001 Human Chondrocyte 96 reactions . . . . . . . . . . . . . . . . 181 VPA-1012 Mouse Dendritic (Mat .), 25 reaction . . . . . . . . . . . . . . 143
VHPF-2001 Human Chondrocyte 960 reactions . . . . . . . . . . . . . . 181 VPB-1001 Human Coronary Artery Endothelial Cell . . . . . . . . . . 156
VHPG-1003 Rat Neuron 96 reactions . . . . . . . . . . . . . . . . . . . . . . . . . 168 25 reactions
VHPG-2003 Rat Neuron 960 reactions . . . . . . . . . . . . . . . . . . . . . . . 168 VPB-1002 Human Umbilical Vein Endothelial Cell 25 reactions 157
VHPH-1001 Mouse Embryonic Stem 96 reactions . . . . . . . . . . . . . 177 VPB-1003 Human Microvascular Endothelial Lung Cell
VHPH-2001 Mouse Embryonic Stem 960 reactions . . . . . . . . . . . . 177 25 reactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
VHPH-5002 Basic Starter Kit for Human Stem Cells . . . . . . . . . . . . 175 VPC-1001 Human Aortic Smooth Muscle Cell 25 reactions . . . 172
VHPH-5003 Human Stem Cell (H9) Kit . . . . . . . . . . . . . . . . . . . . . . . 176 VPD-1001 Human Dermal Fibroblast 25 reactions . . . . . . . . . . . 151
VHPH-5222 Basic Nucleofector® Kit 2 . . . . . . . . . . . . . . . . . . . . . . . . 175 VPD-1002 Human Keratinocyte 25 reactions . . . . . . . . . . . . . . . 152
for Human Stem Cells, 960 reactions VPD-1003 Human Neonatal Melanocytes (NHEM-Neo) . . . . . . 153
VHPH-5012 Basic Nucleofector® Kit 1 . . . . . . . . . . . . . . . . . . . . . . . . 175 25 reactions
for Human Stem Cells, 96 reactions VPD-1004 Mouse Embryonic Fibroblast . . . . . . . . . . . . . . . . . . . . 182
VHPH-5203 Human Stem Cell (H9) Kit . . . . . . . . . . . . . . . . . . . . . . . 176 Nucleofector® Kit 1, 25 reactions
VHPH-5212 Basic Nucleofector® Kit 1 for Human Stem Cells . . . 175 VPD-1005 Mouse Embryonic Fibroblast . . . . . . . . . . . . . . . . . . . . 182
960 reactions Nucleofector® Kit 2, 25 reactions
VHPH-5022 Basic Nucleofector® Kit 2 for Human Stem Cells . . . 175 VPD-1006 Mouse Embryonic Fibroblasts Starter 10 reactions 182
VHPI-1002 Basic Starter Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 VPE-1001 Human Mesenchymal Stem Cell 25 reactions . . . . . 178
for Primary Mammalian Fibroblasts, 64 reactions VPE-1002 Rat Cardiomyocyte Neonatal 25 reactions . . . . . . . . 180
VHPI-1003 Basic Kit for Primary Mammalian Neurons, . . . . . . . 170 VPF-1001 Human Chondrocyte 25 reactions . . . . . . . . . . . . . . . . 181
96 reactions VPG-1001 Mouse Neuron 25 reactions . . . . . . . . . . . . . . . . . . . . . . 167
VHPI-1004 Basic Kit for Primary Smooth Muscle Cells, . . . . . . . . 173 VPG-1002 Chicken Neuron 25 reactions . . . . . . . . . . . . . . . . . . . . 167
96 reactions VPG-1003 Rat Neuron 25 reactions . . . . . . . . . . . . . . . . . . . . . . . . . 168
VHPI-1005 Basic Nucleofector® for Primary . . . . . . . . . . . . . . . . . . 163 VPG-1004 Mouse Neural Stem Cell 25 reactions . . . . . . . . . . . . . 179
Mammalian Epithelial Cells, 64 reactions VPG-1005 Rat Neural Stem Cell 25 reactions . . . . . . . . . . . . . . . . 179
VHPI-1012 Basic Kit 1 for Primary Mammalian Fibroblasts, . . . 155 VPG-1006 Mouse Astrocyte 25 reactions . . . . . . . . . . . . . . . . . . . 166
96 reactions VPG-1007 Rat Astrocyte 25 reactions . . . . . . . . . . . . . . . . . . . . . . . 166
VHPI-1015 Basic Nucleofector® Kit 1 for Primary . . . . . . . . . . . . 163 VPG-1009 Rat Oligodendrocyte 25 reactions . . . . . . . . . . . . . . . . 169
MammalianEpithelial Cells, 96 reactions VPH-1001 Mouse Embryonic Stem Cell 25 reactions . . . . . . . . . 177
VHPI-1022 Basic for Primary Mammalian Fibroblasts, . . . . . . . . 155 VPH-5002 Human Stem Cell Starter 18 reactions . . . . . . . . . . . . 174
96 reactions VPH-5012 Human Stem Cell Kit 1, 25 reactions . . . . . . . . . . . . . . 174
VHPI-1025 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . 163 VPH-5022 Human Stem Cell Kit 2, 25 reactions . . . . . . . . . . . . . . 174
Mammalian Epithelial Cells, 96 reactions VPI-1001 Basic Kit for Primary Mammalian Endothelial Cells, 158
VHPI-2003 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . 170 25 reactions
Mammalian Neurons, 960 reactions VPI-1002 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . . 154
VHPI-2004 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . 173 Fibroblasts, 25 reactions
Smooth Muscle Cells, 960 reactions VPI-1003 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . . 169
VHPI-2012 Basic Nucleofector® Kit 2 for Primary . . . . . . . . . . . . 155 Neural Cells, 25 reactions
Mammalian Fibroblasts, 960 reactions VPI-1004 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . 173
VHPI-2015 Basic Nucleofector® Kit 1 for Primary . . . . . . . . . . . . 163 Smooth Muscle Cells, 25 reactions
Mammalian Epithelial Cells, 960 reactions VPI-1005 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . . 163
VHPI-2022 Basic for Primary Mammalian Fibroblasts, . . . . . . . . 155 Mammalian Epithelial Cells, 25 reactions
960 reactions VPK-1001 Normal Human Bronchial Epithelial 25 reactions . . 159
VHPI-2025 Basic for Primary Epithelial Cells, 960 reactions . . . 163 VPK-1002 Human Mammary Epithelial Cell 25 reactions . . . . . 160
VHPK-1001 Human Bronchial Epithelial 96 reactions . . . . . . . . . . 159 VPK-1003 Human Prostate Epithelial Cell 25 reactions . . . . . . . 162
VHPK-1002 Human Mammary Epithelial Cell 96 reactions . . . . . 161 VPL-1002 Mouse Hepatocyte 25 reactions . . . . . . . . . . . . . . . . . . 164
VHPK-1003 Human Prostate Epithelial Cell 96 reactions . . . . . . . 162 VPL-1003 Rat Hepatocyte 25 reactions . . . . . . . . . . . . . . . . . . . . . 165
VHPK-2001 Human Bronchial Epithelial 960 reactions . . . . . . . . 159 VSC-1001 siRNA Test Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200
VHPK-2002 Human Mammary Epithelial Cell 960 reactions . . . . 161 VSPI-1003 Basic Neuron SCN 25 reactions . . . . . . . . . . . . . . . . . . .171
Indices
VHPK-2003 Human Prostate Epithelial Cell 960 reactions . . . . . . 162 VZB-1003 Human Monocyte Nucleofector® Medium, . . . .146, 147
VHPL-1001 Human Hepatocyte 96 reactions . . . . . . . . . . . . . . . . . 164 500 ml
VHPL-1002 Human Hepatocyte 960 reactions . . . . . . . . . . . . . . . . 164 VZB-4003 Human Monocyte Nucleofector® Medium, . . . .146, 147
VMI-1001 Basic Parasite Starter Nucleofector® Kit . . . . . . . . . . . 188 4 x 500 ml
VMI-1011 Basic Parasite Starter Nucleofector® Kit 1 . . . . . . . . . 188 W50-100 LAL Reagent Water 100 ml . . . . . . . . . . . . . . . . . . . . . . . 330
VMI-1021 Basic Parasite Starter Nucleofector® Kit 2 . . . . . . . . . 188 W50-500 LAL Reagent Water 500 ml . . . . . . . . . . . . . . . . . . . . . . . 330
VPA-1001 Human B Cell Nucleofector® Kit . . . . . . . . . . . . . . . . . . 140 W50-640 LAL Reagent Water 30 ml . . . . . . . . . . . . . . . . . . . . . . . . 330
VPA-1002 Human T Cell 25 reactions . . . . . . . . . . . . . . . . . . . . . . . 149
357
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Alphabetical Index
96-well Shuttle® Automation Package . . . . . . . . . . . . . . . . . . . . . . . . .135 - 137 L-15 (Leibovitz) Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
HeLaS3 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . . . . . .135 McCoy’s 5A Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
Neuro2a 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . . . . 135 Minimum Essential Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256 - 257
HeLa 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . . . . . . . .135 Medium 199 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
NCTC-109 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
■ A Richter’s CM Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
RPMI 1640 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
ACK Lysing Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .286, 236 Schneiders' Drosophila Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
AdipoRed™ Assay Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91, 236 TC 100 Insect Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
Adult Human Dermal Blood Microvascular Endothelial Cells . . . . . . . . . . .29 Waymouth's MB 752/1 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
Adult Human Dermal Lymphatic Microvascular Endothelial Cells . . . . . .28 Williams' Medium E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
Adult Human Dermal Microvascular Endothelial Cells . . . . . . . . . . . . . . . . .28 Bladder Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21 - 22
Adult Normal Human Epidermal Keratinocytes . . . . . . . . . . . . . . . . . . . . . . . 41 BdSMC – Human Bladder Smooth Muscle Cells . . . . . . . . . . . . . . . 22, 65
Airway Epithelial Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 - 20 Bladder Microvascular Endothelial Cell Growth Media . . . . . . . . . . . . . .22
BEGM® Bronchial Epithelial Cell Growth Media . . . . . . . . . . . . . . . . . 19, 20 Bladder Smooth Muscle Cell Growth Media . . . . . . . . . . . . . . . . . . . . . . .22
NHBE – Normal Human Bronchial/Tracheal Epithelial Cells . . . . . . . . . 19 HMVEC-Bd – Human Bladder Microvascular Endothelial Cells . . . . . .22
SAEC – Human Small Airway Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . 19 Bladder Microvascular Endothelial Cell Growth Media . . . . . . . . . . . . . . . . .22
SAGM™ Small Airway Epithelial Cell Growth Media . . . . . . . . . . . . . 19, 20 Bladder Smooth Muscle Cell Growth Media . . . . . . . . . . . . . . . . . . . . . . . 22, 65
Amaxa® Nucleofection® and Transfection . . . . . . . . . . . . . . . . . . . . . .126 - 127 SmBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65
Amaxa® Nucleofector® Technology . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 - 131 SmGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65
Amniochrome® II Modified Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273 SmGM®-2 SingleQuots® Supplements and Growth Factors . . . . . 22, 65
Amniochrome® Plus Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273 Blood Brain Barrier Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Amniochrome® Pro Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273 bMVEC-B – Bovine Brain Microvascular Endothelial Cells . . . . . . . . . . . . . . 13
Amphotericin B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .199, 285 Bone Marrow and Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 - 85
Animal and Human Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289 - 297 Human Cord Blood Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .84
Handling Serum Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 292 Human Bone Marrow Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . .84
Inactivation of Viruses and Mycoplasma by Gamma Irradiation . . 292 Normal Human Peripheral Blood Leukopaks . . . . . . . . . . . . . . . . . . . . . .83
Origin and Types of Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .291 Quadratox 4 Species Panel of Bone Marrow Mononuclear Cells . . . . .85
Quality Assurance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 290 Unprocessed Human Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Serum Tests for Special Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . .291 Bone Marrow CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Special Handling Fees . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 Bone Marrow Non-Irradiated Stromal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . .89
Special Serum Treatments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 Bovine Aortic Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70
Animal Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 - 77 Bovine Aortic Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . . . . . . . .70
Annexin V, Alexa Fluor® 488 Conjugate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244 Bovine Brain Extract (BBE) 9 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Antibiotics and Antimycotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .199, 285 Bovine Brain Microvascular Endothelial Cell Growth Media . . . . . . . . . . . . 13
AoAF – Human Aortic Adventitial Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . 37 Bovine Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
AoSMC – Human Aortic Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . 65 Bovine Pituitary Extract (BPE) 13 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
ApoGlow® Rapid Apoptosis Screening . . . . . . . . . . . . . . . . . . . . . . . . . 228 - 229 Bovine Pulmonary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Ascorbic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60 Bovine Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
Ascorbic Acid 25 .5 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
ATP Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 Calf Serum, Newborn . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
bPAEC – Bovine Pulmonary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . 71
■ B Bronchial Epithelial Cell Growth Media . . . . . . . . . . . . . . . . . . . . . . . . . . . 19, 20
BSMC – Normal Human Bronchial Smooth Muscle Cells . . . . . . . . . . . . . . .66
b-G-Blocker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330 Buffers and Buffered Salines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 - 287
bAEC – Bovine Aortic Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . . . . .70
bAEC – Bovine Aortic Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . .70 ■ C
Basal Medium Eagle (BME) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253
Basic Parasite Nucleofector® Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .188 Calcium Chloride Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
bCAEC – Bovine Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . 71 CalciFluor™ Quantitative Bone Resorption Assay . . . . . . . . . . . . . . . . . . . . .239
BdSMC – Human Bladder Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . 22, 65 Calcium Chloride 300 mM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
BEGM® Bronchial Epithelial Cell Growth Media . . . . . . . . . . . . . . . . . . . . 19, 20 Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
BioAssay Accessory Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 CASMC – Human Coronary Artery Smooth Muscle Cells . . . . . . . . . . . . . . .66
BioAssays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217 - 219 CD14+ Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93
BioAssays FAQs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247 - 248 CD34+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Bioservices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 246 CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Cell Analysis Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242 - 244 Cell Culture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281 - 287
G Protein-Coupled Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .245 Balanced Salt Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282
Indices
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QCL-1000® Endpoint Chromogenic LAL Assay . . . . . . . . . . . . . . . . . . . 328 Validation Test Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .335
Epidermal Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 Flx 800LBS Fluorescent Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
Epidermal Keratinocyte Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . 40 - 44
NHEK-Ad – Adult Normal Human Epidermal Keratinocytes . . . . . . . . . 41 ■ G
NHEK-Neo – Neonatal Normal Human Epidermal Keratinocytes . . . .42
NHEK-Neo – Neonatal Normal Human Epidermal . . . . . . . . . . . . . . . . .43 G Protein Coupled Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .245
Keratinocytes, Pooled Grace's Insect Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255
Epidermal Keratinocyte Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 Gel Clot LAL Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326
KBM®-2 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 PYROGENT® Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326
KBM®-2 w/o Ca++ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 PYROGENT® Plus Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . 326
KBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 PYROGENT® Ultra Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . .327
KBM®-CD Keratinocyte Basal Medium, Chemically Defined . . . . . . . . .44 Gene Expression Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209 - 216
KBM® Basal Medium w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 Gentamicin Sulfate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285
KGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41, 42, 43, 44 Glasgow Minimum Essential Medium (GMEM) . . . . . . . . . . . . . . . . . . . . . .255
KGM®-2 BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
KGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . .44 ■ H
KGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
KGM® BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 HAEC – Human Aortic Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
KGM®-CD BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 Ham's Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255
KGM®-CD SingleQuots® Supplements and Growth Factors . . . . . . . . . .44 Hanks' BSS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282
KGM® Complete System (supplemented) . . . . . . . . . . . . . . . . . . . . . . . .44 HCAEC – Human Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . .25
KGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .44 hEGF 3 µg/ml Epidermal Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 Hematopoietic Progenitor Cells and Media . . . . . . . . . . . . . . . . . . . . . . .86 - 89
Epidermal Melanocyte Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 - 46 Hepatocyte Culture Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15, 17
NHEM-Neo – Neonatal Normal Human Epidermal Melanocytes . . . .46 Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Epidermal Melanocyte Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 HEPES Buffered Saline Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78, 286
ET-3 Growth Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 hFGF 1 µg/ml Human Fibroblastic Growth Factor . . . . . . . . . . . . . . . . . . . . . 78
MBM®-4 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 HIAEC – Human Iliac Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . .26
MGM®-4 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 HiFect® Transfection Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .191
MGM®-4 SingleQuots® Supplements and Growth Factors . . . . . . . . . . .46 HL-1™ Chemically Defined, Serum-free Medium . . . . . . . . . . . . . . . . 274 - 275
Phosphate Buffered Saline (PBS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 HMEC – Human Mammary Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . .48
Equine Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 hMSC Human Mesenchymal
Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 hMSC Mesenchymal Stem Cell Adipogenic Differentiation BulletKit® . . .90
Erythroid Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88 hMSC Mesenchymal Stem Cell Chondrogenic
ET-3 Growth Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Differentiation BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90
ExPro™ Luminometer Injector Cleaning Solution . . . . . . . . . . . . . . . . . . . . .241 hMSC Mesenchymal Stem Cell Osteogenic Differentiation BulletKit® . . .90
HMVEC-Bd – Human Bladder Microvascular Endothelial Cells . . . . . . . . . .22
■ F HMVEC-C – Human Cardiac Microvascular Endothelial Cells . . . . . . . . . . . 31
HMVEC-dAd – Adult Human Dermal Microvascular Endothelial Cells . . . .28
Fetal Bovine Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 HMVEC-dBlAd – Adult Human Dermal Blood
Fetal Bovine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Fetal Bovine Serum (Available Outside USA and Canada) . . . .296, 297 HMVEC-dBlNeo – Neonatal Human Dermal Blood
Fetal Liver CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
Fibroblast Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36 - 39 HMVEC-dLyAd – Adult Human Dermal Lymphatic
AoAF – Human Aortic Adventitial Fibroblasts . . . . . . . . . . . . . . . . . . . . . 37 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
HPdLF – Human Periodontal Ligament Fibroblasts . . . . . . . . . . . . . . . . 37 HMVEC-dLyNeo – Neonatal Human Dermal Lymphatic
NHDF-Ad – Human Adult Dermal Fibroblasts . . . . . . . . . . . . . . . . . . . . . .38 Microvascular Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . .30
NHDF-Neo – Human Neonatal Dermal Fibroblasts . . . . . . . . . . . . . . . . .38 HMVEC-dNeo – Neonatal Human Dermal
NHLF – Human Lung Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Microvascular Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . . . . . . . 31
Fibroblastic Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
HMVEC-dNeo – Neonatal Human Dermal
Fibroblast Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
Microvascular Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . .29
FBM® Fibroblast Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
HMVEC-LBl – Human Lung Blood
FGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38, 39
Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
FGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . .39
HMVEC-L – Human Lung Microvascular Endothelial Cells . . . . . . . . . . . . .26
FGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
HMVEC-LLy – Human Lung Lymphatic Microvascular Endothelial Cells 27
FGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .39
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 h NHEPS® – Human Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Hoechst 33342 Nuclear Counterstain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .243
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361
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Cell Line Optimization 96-well Nucleofector® Kit . . . . . . . . . . . . . . . . .187 List of Nucleofector® Kits for Primary Cells . . . . . . . . . . . . . . . . . 138, 139
High Throughput Nucleofection® Nucleofector® Kits of Cell Lines . .185 MEF (Mouse Embryonic Fibroblast) . . . . . . . . . . . . . . . . . . . . . . . . . . . .182
Nucleofection® of Cell Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185 - 187 Neural Cell Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166 - 169
Optimized Protocols for Cell Lines . . . . . . . . . . . . . . . . . . . . . . . . 183 - 184 Basic Neuron SCN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
Nucleofector® Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132 Chicken Neuron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167
96-well Shuttle® Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 Mouse Astrocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .166
96-well Shuttle® Device Extended Guarantee and Service . . . . . . . .134 Mouse Neuron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167
Nucleofector® Device Extended Guarantee and Service . . . . . . . . . . .134 Primary Mammalian Neurons (Basic) . . . . . . . . . . . . . . . . . . . . . .169
362
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Primary Mammalian Neurons 96-well . . . . . . . . . . . . . . . . . . . . . .170 hEGF 3 µg/ml Epidermal Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Astrocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 HEPES Buffered Saline Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Neuron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 hFGF 1 µg/ml Human Fibroblastic Growth Factor . . . . . . . . . . . . . . . . . . 78
Rat Neuron 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 Human Transferrin 10 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Oligodendrocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169 NSF-1 Neural Survivor Factor-1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Cardiomyocyte Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . 180 ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Cardiomyocyte – Neonatal . . . . . . . . . . . . . . . . . . . . . . . . . . . 180 Retronectin Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Smooth Muscle Cells Nuceofector Kits . . . . . . . . . . . . . . . . . . . . .172 - 173 Retronectin® Recombinant Human Fibronectin Fragment . . . . . . . . . 78
Human Aortic Smooth Muscle Cell (Basic) . . . . . . . . . . . . . . . . . . 172 Trypsin/EDTA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Primary Smooth Muscle Cells (Basic) . . . . . . . . . . . . . . . . . . . . . .173 Trypsin Neutralizing Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Primary Smooth Muscle Cells 96-well (Basic) . . . . . . . . . . . . . . .173 PrimeFect™ DNA and siRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 - 204
Stem Cell Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .174 - 179 Primary Neural Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 - 77
Human CD34+ Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .176 Mouse C57 Brain Cortex Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Human MSC (Mesenchymal Stem Cell) . . . . . . . . . . . . . . . . . . . . .178 Mouse C57 Brain Striatum Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Human Stem Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174 Mouse CD1 Brain Cortex Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Human Stem Cell (H9) 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .176 Mouse CD1 Brain Mixed Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Human Stem Cells 96-well (Basic) . . . . . . . . . . . . . . . . . . . . . . . . .175 Mouse CD1 Brain Striatum Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Mouse ES Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 Mouse CD57 Brain Mixed Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Mouse ES Cell 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 Rat Brain Cortex Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Mouse NSC (Neural Stem Cell) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .179 Rat Brain Cortex Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Rat NSC (Neural Stem Cell) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .179 Rat Brain Cx-Hi-Cp Mix Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Rat Brain Hippocampus Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
■ O Rat Brain Hippocampus Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Rat Brain Striatum Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
OCP Osteoclast Precursor Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Rat Brain Striatum Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Optimized Protocols for Cell Line Transfection . . . . . . . . . . . . . . . . . 183 - 184 Rat Cerebellar Neurons (Granular Cells) . . . . . . . . . . . . . . . . . . . . . . . . . . 74
OsteoAssay™ Human Bone Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .237 Rat Dorsal Root Ganglion Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Osteoclast Precursor Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Primary Neuronal Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74 - 77
OsteoImage™ Mineralization Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 240 ABM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
OsteoLyse™ Assay Kit (Human Collagen) . . . . . . . . . . . . . . . . . . . . . . . . . . .238 AGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 - 77
AGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .77
■ P PNBM™ Primary Neuron Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . .77
PNGM™-A Primary Neuron Growth Medium - Adult BulletKit® . . . 74, 77
PASMC – Human Pulmonary Artery Smooth Muscle Cells . . . . . . . . . . . . . 67 PNGM™-A Primary Neuron Growth
PBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46, 287 Medium - Adult SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
PDELight® HTS cAMP Phosphodiesterase . . . . . . . . . . . . . . . . . . . . . 232 - 233 PNGM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
Penicillin-Streptomycin Mixture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285 PNGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74, 76 - 77
Penicillin-Streptomycin-Amphotericin B Mixture . . . . . . . . . . . . . . . . . . . . 285 PNGM™ SingleQuots® Supplement and Growth Factor . . . . . . . . . . . . . .77
Penicillin-Streptomycin -L-glutamine Mixture . . . . . . . . . . . . . . . . . . . . . . 285 ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
Peptide Transfection Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .201 Pro293™ Chemically Defined, Serum-free Media . . . . . . . . . . . . . . . . . . . . .270
Peripheral Blood CD14+ Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 ProCHO™ Protein-free CHO Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .267
Phalloidin, Alexa Fluor® 488 Conjugate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242 ProDoma™ Serum-free Hybridoma Media . . . . . . . . . . . . . . . . . . . . . . . . . . .277
Phosphate Buffered Saline (PBS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) . . . . . 269
PKLight® HTS Protein Kinase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230 - 231 Progenitor Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
pmaxFP® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192 HPGM™ Hematopoietic Progenitor Growth Medium . . . . . . . . . . . . . . . .87
pmaxFP®-Green . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .194 Neural Progenitor Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
pmax®-Yellow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195 NPBM™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
pmax®-Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196 NPDM™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
pmaxCloning™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 NPMM™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
Poietics® FAQs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 ProHT™ Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
Poietics® Technical Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 ProNS0™ Chemically Defined, Protein-free Media . . . . . . . . . . . . . . . . . . . .279
Human Mesenchymal Stem Cell Media . . . . . . . . . . . . . . . . . . . . . . . . . .123 ProPer™ 1 Chemically Defined, Serum-free Medium . . . . . . . . . . . . . . . . . .271
Neural Progenitor Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 Prostate Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 - 53
Osteoclast Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 PrEC – Human Prostate Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . .52
Preadipocyte Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 PrSC – Human Prostate Stromal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
Procedure for Thawing CD34+ and Precursor Cells . . . . . . . . . . . . . . . .122 PrSMC – Human Prostate Smooth Muscle Cells . . . . . . . . . . . . . . . 53, 67
PowerCHO® Chemically Defined, Serum-free CHO Media . . . . . . . . . . . . . 268 Prostate Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
PowerCHO® -GS Chemically Defined, Serum-free CHO Media . . . . . . . . . 268 PrEBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67
Indices
363
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Alphabetical Index
Continued
SmGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . .53 SmGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .53
PrSC – Human Prostate Stromal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 Schneiders' Drosophila Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
PrSMC – Human Prostate Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . 53, 67 Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289 - 297
PyroGene® Recombinant Factor C Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . .329 Serum Supreme . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
PYROGENT® Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326 siRNA Test Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200
PYROGENT® Plus Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326 Skeletal Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58, 60
PYROGENT® Ultra Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .327 NHAC-kn – Normal Human Articular Chondrocytes, Knee . . . . . . . . . .59
PYROSPERSE® Dispensing Agent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330 NHOst – Normal Human Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . .59
Skeletal Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
■ Q Ascorbic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Calcium Chloride Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
QCL-1000® Endpoint Chromogenic LAL Assay . . . . . . . . . . . . . . . . . . . . . . 328 CBM Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Quadratox 4 Species Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CDM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Baboon Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CDM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Canine Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CDM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .60
Human Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59, 60
Murine Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .60
Quick Reference - Primary Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . 3 - 6 Chondrocyte ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . .60
Sodium Chloride Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
■ R OBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
OGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59, 60
Rat Cardiac Myocyte Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
OGM™ Differentiation SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . .60
RCGM Rat Cardiac Myocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
OGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .60
Rat Cardiac Myocyte Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
RCBM Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
SkBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
RCGM BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Sodium Alginate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
RCGM SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Sodium Citrate Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Rat Hepatocytes – Sprague-Dawley & Media . . . . . . . . . . . . . . . . . . . . . . . . . 17
Skeletal Muscle Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 - 63
Ready Heps™ Fresh Human Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
HSMM – Human Skeletal Muscle Myoblasts . . . . . . . . . . . . . . . . . . . . . .62
ReagentPack™ Subculture Reagents
SkMC – Human Skeletal Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
. . . . . . . . . . . . . . . . . . . 20, 35, 39, 44, 48, 50, 53, 57, 60, 63, 68, 77, 78
Skeletal Muscle Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
REGM™ Renal Epithelial Cell Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . 11
DPBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
REBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
REGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11, 55
SkBM®-2 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
REGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . 11
SkGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62 - 63
Renal Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54 - 57
SkGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . 63
HRCE – Human Renal Cortical Epithelial Cells . . . . . . . . . . . . . . . . . . . . .55
SkGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62, 63
HRE – Human Renal Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
SkGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . 63
NHMC – Normal Human Mesangial Cells . . . . . . . . . . . . . . . . . . . . . . . . .56
SkMC – Human Skeletal Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
RPTEC – Human Renal Proximal Tubule Epithelial Cells . . . . . . . . . . . .56
Smooth Muscle Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 - 68
Renal Epithelial Cell Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10, 11
AoSMC – Human Aortic Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . 65
Plated cells – polyester Transwell® Membranes . . . . . . . . . . . . . . . . . . 11
BdSMC – Human Bladder Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . 65
Renal Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
BSMC – Normal Human Bronchial Smooth Muscle Cells . . . . . . . . . . .66
MsBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
CASMC – Human Coronary Artery Smooth Muscle Cells . . . . . . . . . . . .66
MsGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 - 57
PASMC – Human Pulmonary Artery Smooth Muscle Cells . . . . . . . . . . 67
MsGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . 57
PrSMC – Human Prostate Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . 67
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
UASMC – Human Umbilical Artery Smooth Muscle Cells . . . . . . . . . . . .68
REBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
UtSMC – Human Uterine Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . .68
REGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55 - 57
Smooth Muscle Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65 - 68
REGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . 57
PrEGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Retronectin Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78, 204
PrEBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Retronectin® Recombinant Human Fibronectin Fragment . . . . . . . . 78, 204
PrEGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . 67
rhTGF Beta 3 for hMSC Chondrogenic Differentiation
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68
Media Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90
SmBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 - 68
Richter’s CM Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
SmGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 - 68
RPMI Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 - 259
SmGM®-2 SingleQuots® Supplements and Growth Factors . .22, 65, 68
RPTEC – Human Renal Proximal Tubule Epithelial Cells . . . . . . . . . . . . . . . .56
Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
rt NHEPS® – Rat Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Sodium Alginate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Indices
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Alphabetical Index
Continued
■ T
TC 100 Insect Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
TL HEPES Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .287
ToxiLight® Non-destructive Cytotoxicity . . . . . . . . . . . . . . . . . . . . . . 226 - 227
Transfection Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .191
HiFect® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .191
PrimeFect™ DNA and siRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 - 204
Transport™ Protein Delivery Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . 202
Transport™ Reagents FAQ's . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205
Transfection Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 342
Transport™ Protein Delivery Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 202
Transport™ Reagents FAQ's . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205
Tris Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
Trypan Blue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
Trypsin 1:250 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284
Trypsin/EDTA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78, 284
Trypsin/EDTA (for use with human primary cells) . . . . . . . . . . . . . . . . . . . .90
Trypsin/EDTA Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
Trypsin-Versene® (EDTA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284
Trypsin Neutralizing Solution (TNS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46, 78
■ U
Indices
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Notes
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Notes
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