Lonza Celldiscovery

Cell Discovery
Research Products Catalog

2009–2010
Clonetics® Primary Cells and Media

Poietics® Stem Cells and Media
Amaxa® Nucleofection® and Transfection
BioWhittaker® Media and Sera
StellARray™ qPCR Arrays
MycoAlert®, ToxiLight® and ViaLight®
Cell-based Assays
Endotoxin Detection Products
Flexible BioProcess Containers
Dear Valued Lonza Customer,
We are pleased to introduce the 2009 - 2010 Lonza Cell Discovery Research Products Catalog. Inside, you will find all of
your known and trusted Lonza research products and services, as well as many new offerings designed to advance and
accellerate your research:
■ Clonetics® Primary Cells and Media - over 100 authenticated primary cell types and
optimized media, ready to use today
■ Poietics® Stem Cells and Media – authenticated adult stem cells available with
differentiation media
■ New! Amaxa® Nucleofector® Transfection Technology – unique technology for the

efficient, non-viral transfection of DNA or siRNA
■ MycoAlert® Mycoplasma Detection System - detect mycoplasma contamination in your

cell cultures in less than 20 minutes
■ BioWhittaker® Media and Sera – classical sera and media, plus an extensive array of
serum free media including protein-free, chemically defined and non-animal
derived formulas
Look for Lonza Molecular Biology Products in the 2009 - 2010 Molecular Biology Research Products Catalog and
Sourcebook for Electrophoresis. The Molecular Biology catalog features our known and trusted products, such as
SeaKem®, NuSieve® and MetaPhor® Agarose; The FlashGel® System; and PAGEr® Precast Protein Gels, as well as our new
Sourcebook for Electrophoresis, featuring protocols and support tools for your most critical molecular biology techniques.
Request a copy of this catalog at www.lonza.com/research.
Purchase any of our products directly from Lonza by calling us at the number provided on the back of this catalog,
or use our e-commerce capabilities at www.lonza.com/research.
Should you require technical information, please contact our Scientific Support Team at the phone number provided on the
back of this catalog, or at scientific.support.eu@lonza.com.
Lonza is committed to providing the highest quality products and services, and to helping researchers worldwide
advance and accellerate life science research. For all of your cell discovery and molecular biology needs, turn to Lonza. We
appreciate your continued support and look forward to serving you.
The Lonza Cell Discovery Team
32 (0) 87 321 611 www.lonza.com/research

Table of Contents
Table of Contents
Customer Service iii Poietics® Stem Cells and Media
Terms and Conditions v Introduction 81
Trademarks vii Donor Programs 82
E-commerce inside back cover Bone Marrow and Mononuclear Cells 83
Hematopoietic Progenitor Cells and Media 86
Chapter 1 – Clonetics® and Poietics® Mesenchymal Stem Cells and Media 90
Primary Cells and Media Preadipocyte Cells and Media 91
Osteoclast Precursor Cells and Media 92
Clonetics® & Poietics® Quick Reference Guide 3 Immune System Cells and Media 93
Neural Progenitor Cells Media 97
Clonetics® Primary Cells and Media Custom Cell Isolation Services 98
Introduction 7
Clonetics® Conditionally Immortalized Cell Lines 8 Cell Biology Technical Information
Clonetics® Cell Models 9 Clonetics® Frequently Asked Questions 99
Clonetics® Technical Information 101
Hepatocytes and Media Clonetics® Media 111
Human Hepatocytes & Media 14 Poietics® Frequently Asked Questions 121
Poietics® Technical Information 122
Rat Hepatocytes & Media 17
Poietics® Media 123
Human Cells and Media
Chapter 2 – Amaxa® Nucleofection® and Transfection
Airway Epithelial Cells and Media 18
Introduction 127
Bladder Cells and Media 21 Amaxa® Nucleofector® Technology 128
Endothelial Cells and Media 23 Nucleofector® II Device 132
Fibroblast Cells and Media 36 96-well Shuttle® Device 133
Epidermal Keratinocyte Cells and Media 40 Nucleofector® Extended Guarantee and
Epidermal Melanocyte Cells and Media 45 Service Contracts 134
Mammary Epithelial Cells and Media 47
96-well Shuttle® Automation Package 135
Neural Cells and Media 49
Nucleofector® Kits for Primary Cells 138
Prostate Cells and Media 51
Renal Cells and Media 54 Nucleofector® Kits for Cell Lines 183
Cell Line Nucleofector® Kits 185
Skeletal Cells and Media 58
Cell Line Optimization Nucleofector® Kits 186
Skeletal Muscle Cells and Media 61
cGMP Cell Line Nucleofector® Kits 188
Smooth Muscle Cells and Media 64
Basic Parasite Nucleofector® Kits 188
Animal Cells and Media FAQs on Nucleofection® 189
HiFect® Transfection Reagent 191
Introduction 69 Vectors 192
Endothelial Cells and Media 69
Antibiotics 198
Rat Cardiac Myocytes and Media 72
siRNA Test Kit 200
Primary Neural Cells and Media 73
Peptide Transfection Control 201
Primary Neuron Growth Media 77
Cell Culture Reagents 78 Transport™ Protein Delivery Reagent 202
Custom Cell Services 79 Table of Contents continues on next page
i
Table of Contents
Continued
Table of Contents
PrimeFect™ DNA & siRNA Transfection Reagent 203 Cell Culture Technical Information
Cell Biology Technical Information 101 Adaptation of Cell Cultures to Serum-free Medium 298
Protocols for Weaning Cell Cultures 299
Chapter 3 – Arrays
Cryopreservation and Reconstitution 300
Introduction 209 Determination of Cell Numbers 301
GeneSieve™ Query 210 Powdered Medium Preparation 303
Global Pattern Recognition™ Analysis Tool 211 Subculturing Procedures for Mammalian Cells 304
StellARray™ qPCR Arrays 211 Media FAQs 306
Sera FAQs 307
Chapter 4 – BioAssays
Cell Culture Reagents FAQs 308
Introduction 219
Formulations for BioWhittaker® Classical Media 309
BioAssay Kits 220
Cell Analysis Reagents 242 Chapter 6 – Endotoxin Detection
G Protein-Coupled Receptors 245
Introduction 325
BioAssay Services 246
Gel Clot LAL Assays 326
BioAssay FAQs 247
Endpoint Chromogenic LAL Assay 328
Chapter 5 – Cell Culture Fluorescent Assay 329
LAL Accessory Products 330
Classical Media
Introduction 251 Chapter 7 – Flexible Bioprocess Containers
BioWhittaker® Classical Media 253 Introduction 333
Platinum UltraPAK™ Bioprocess Containers 336
Specialty Media
Bioprocessing Tank Liners 337
Introduction 261
General Purpose Serum-free Media 262 ProEVA PAK™ Bioprocess Containers 338
X-VIVO™ Chemically Defined, Serum-free
Hematopoietic Cell Media 265
Chapter 8 – Lonza Custom Manufacturing
CHO Media 266 Introduction 340
NAO Freezing Medium 269 Lonza Custom Service Contacts 341
Renal Media 269 Transfection Services 342
Specialty Media - IVF 272
Specialty Media - Cytogenetics 273 Chapter 9 – Distributors and Indices
Hybridoma Media 274 Numerical Index 343
Insect-XPRESS™ Protein-free Insect Cell Medium 278 Alphabetical Index 358
ProNS0™ Chemically Defined, Protein-free Media 279
Cell Culture Reagents
Introduction 281
Balanced Salt Solutions 282
Reagents 283
Antibiotics and Antimycotics 285
Buffers and Buffered Saline 286
Animal and Human Sera
Introduction 289
Fetal Bovine Sera 294
Equine Sera 294
Bovine Sera 295
Human Sera 295
Fetal Bovine Sera
– Available Outside the USA and Canada 296
ii
Customer Service
U.S. and Canada
Customer Service
Ordering Information Scientific Support
To place an order, please use any of the following convenient Providing world-class technical support for our products
options: is a top priority. Our Scientific Support Representatives rely
Phone on years of laboratory experience to assist with product
Our Customer Service Representatives are ready to assist selection and help you maximize product performance.
you with ordering Monday through Friday, from 8 a.m. to – In the U.S., call us at (800) 521-0390, Monday
6 p.m. Eastern Standard Time (EST). Call from anywhere in through Friday, between 8 a.m. and 5:30 p.m. EST
the U.S. and Canada at: – Outside the U.S., call (207) 594-3400 for Molecular
Toll Free: (800) 638-8174 Biology Products or (301) 898-7025 for all others
Phone: (301) 898-7025
– You may also reach us by e-mail at
Fax scientific.support@lonza.com
(301) 845-8338
– Full product information, instructions, and MSDS are
Mail also available at www.lonza.com/research
Lonza Walkersville, Inc.
Customer Service Department
8830 Biggs Ford Road
Walkersville, MD 21793 USA
Please include all of the following information on all orders:
– Purchase order number
– Shipping address and billing address
– Contact name and telephone number
– Name and department of end user
– Quote or reserve lot information
– Item number, size, quantity, and name of products
ordered
Online Ordering
Ordering for all of our products is available, 24 hours/day,
7 days per week. Our online ordering system is secure,
fast, and convenient. Registered users enjoy the ability
to see their contracted prices, track orders, and manage
their account. Go to www.lonza.com/research for more
information.
Ordering Information – Outside the U.S.

For current information on Lonza distributors, use our
website, www.lonza.com/dist, or contact your local Lonza
distributor to place your order. In the event that you do
not have an authorized distributor, please contact:
Lonza Walkersville, Inc.

Customer Service Department
8830 Biggs Ford Road
Walkersville, MD 21793 USA
Phone: (301) 898-7025 Fax: (301) 845-8338
iii
Customer Service
International
Customer Service
Ordering Information Please include all of the following information on all orders:
– Purchase order number
To place an order, please use any of the following convenient
– Shipping address and billing address
options:
– Contact name and telephone number
Phone
Our Customer Service Representatives are ready to assist – Name and department of end user
you with ordering Monday through Friday. – Quote or reserve lot information
International Offices – Item number, size, quantity, and name of products
Australia 61 3 9550 0883 ordered
Austria 0800 201 538
Online Ordering
Belgium 00 32 87 321 611
Online ordering for all of our products is available, 24
Brazil 55 11 2069 88 00
hours/day, 7 days per week. Our online ordering system
Denmark 45 43 56 74 00
is secure, fast, and convenient. Registered users enjoy the
France 0800 91 19 81
ability to see their contracted prices, track orders, and
Germany 0800 182 52 87
manage their account. Go to www.lonza.com/research for
India 91 22 6697 2883
more information.
Italy 0039 0363 45710
Japan 81 3 5566 0612
Lonza Distributors and Sales Offices
Poland 48 22 833 87 45
Singapore 65 64914214 For current information on Lonza distributors and sales
Spain 34 902 531 366 offices, use our website, www.lonza.com/dist, or contact
Sweden 020 140 4410 your local Lonza distributor to place your order. In the
Switzerland 0800 83 86 20 event that you do not have an authorized distributor,
The Netherlands 0800 022 4525 please contact:
United Kingdom 44 118 979 5234
Lonza Verviers, S.p.r.l.
Fax Verviers, Belgium
International Offices Phone: 00 32 87 321 611
Ausralia 61 3 9550 0890 Fax: 00 32 87 351 967
Austria 0800 201 536 E-mail: info.europe@lonza.com
Belgium 00 32 87 321 634
Brazil 55 11 2274 00 51 Scientific Support
Denmark 45 43 56 74 03
France 0800 91 19 80 Providing world-class technical support for our products
Germany 0800 182 52 78 is a top priority. Our Scientific Support Representatives rely
India 91 22 6697 2888 on years of laboratory experience to assist with product
Italy 39 0363 344063 selection and help you maximize product performance.
Japan 81 3 5566 0619 You may reach Scientific Support by phone or e-mail:
Poland 48 22 669 39 67 – Phone: 49(0)221-99199-400
Singapore 65 62616182
Spain 34 902 531 363 – E-mail:
Sweden 45 43 56 74 03 – scientific.support.eu@lonza.com
Switzerland 0800 83 86 19 – Full product information, instructions, and MSDS are
The Netherlands 0800 022 4942 also available at www.lonza.com/research.
United Kingdom 44 118 936 9441
iv
Terms and Conditions

World – Lonza Rockland, Inc. / Lonza Walkersville, Inc. / Lonza Sales Ltd
Definitions Returns
These Terms and Conditions apply to Lonza Rockland, Inc., Lonza Walkersville, Products may not be returned for credit without first obtaining authorization
Inc. and Lonza Sales Ltd, as the case may be, herein referred to as “Seller” from a Scientific Support or Customer Service Representative. A restocking
and to all products ordered from the Lonza Cell Discovery Research Products fee may be applied for products returned without authorization or for
Catalog 2009-2010. returns in which the purchaser is at fault. The restocking fee is $50 or 25%
of the purchase price, whichever is greater.
Pricing and Terms
Visa®, American Express®, and MasterCard® credit cards are accepted. Prices Force Majeure
and conditions are subject to change without notice. The price applicable Failure of Seller to make, or purchaser to take, any one or more deliveries
to any order accepted by Seller shall be the price in effect on the date of when due, if caused by (a) fire, storm, flood, strike, lockout, accident, act of
shipment. Payment is due within 30 days following the invoice date. There war or terrorism, riot, civil commotion, embargo or similar circumstances,
is a minimum $50 order requirement for all orders. All claims by purchaser (b) any regulation, law, or restriction of any governmental department,
shall be made by written notice to Seller in accordance with these Terms commission, board, bureau, agency, court, or other instrumentality of any
and Conditions, and no offset or deduction from any invoice is permitted. supranational organization of sovereign states, country, state, province,
Acceptance by Seller of bank draft, check, or other media of payment is territory, commonwealth, municipality, or other political subdivision thereof
subject to immediate collection of the full face amount thereof. (a "Governmental Authority"), any seizure or requisition of product by any
Governmental Authority, or any compliance with a demand or request for
If at any time the financial responsibility of purchaser, or the credit risk such product for purposes of national or supranational defense, (c) inability
involved, shall become unsatisfactory to Seller, Seller may require cash or of Seller to obtain any required raw material, energy source, equipment,
satisfactory security prior to subsequent shipments or deliveries hereunder. labor or transportation, at prices and on terms deemed (by Seller) to be
The election by Seller to require such cash or security shall not affect the practicable, from Seller's usual sources of supply, or (d) any other cause
obligation of purchaser to take and pay for the contracted materials. Purchaser or contingency beyond the reasonable control of that party (whether
agrees to pay all costs and expenses, including reasonable attorneys' fees, or not of the same kind or nature as the causes or contingencies above
incurred by Seller in the collection of any sum payable by purchaser to Seller. enumerated), shall not subject the party failing to perform to any liability to
the other during the period such inability to make or take delivery shall exist.
If purchaser breaches any term of the Terms and Conditions or any other Quantities so affected may, at the option of either party, be eliminated from
contractual obligation in favor of Seller, (a) Seller may choose to defer any or the purchase without liability, but the Terms and Conditions shall remain
all further shipments or other performance of any other contractual obligation otherwise unaffected.
in favor of purchaser until purchaser cures its breach, and (b) Seller may, by
delivery of written notice to purchaser describing the breach, immediately In the event of Seller's inability, for any reason, to supply the quantities of
terminate any other contractual obligation to purchaser; provided that product contemplated by the purchase, Seller may allocate its available
purchaser shall have ten (10) days after receipt of the written notice to supply among its purchasers, including departments and divisions of Seller,
reinstate any terminated contractual obligations by curing the breach. In on such basis as Seller may deem fair and practical without liability to
the event of a termination, all outstanding payment obligations or other purchaser for any failure of performance that may result therefrom.
indebtedness of purchaser to Seller shall be due and payable no later than
fifteen (15) days after delivery of notice of termination, subject to the right
of reinstatement.
Indemnification
Seller will make available to purchaser a Material Safety Data Sheet (MSDS)
for each product delivered to purchaser where required. The MSDS sets
Notwithstanding any provision in these Terms and Conditions, Seller shall
forth information concerning such product and describes precautions, if
have no obligation to pay any rebate, issue any credit or make any other
required, to be taken in the transportation, delivery, unloading, discharge,
payment of any kind to purchaser unless purchaser is fully in compliance
storage, handling and use of such product. Purchaser will familiarize itself
with its payment and other obligations under the purchase and any other
with all information and precautions, including but not limited to such
contractual obligation in favor of Seller. In addition, in the event that purchaser
related to safety and health, contained in MSDSs or otherwise transmitted
fails to make any payment when due, Seller shall have the right to offset any
to purchaser by Seller at any time. Seller will instruct its employees, agents,
and all outstanding payment obligations or other indebtedness of purchaser
contractors, customers or any third party which may be exposed to the
to Seller against any outstanding payment obligations or other indebtedness
product about such information and precautions and make available copies
that Seller or any of its affiliates may owe purchaser.
thereof to such parties. Purchaser assumes full liability and responsibility
for compliance with the above-referenced information and precautions, and
Taxes with all laws, statutes, ordinances and regulations of any Governmental
In addition to the purchase price, purchaser shall pay Seller any and all Authority applicable to the processing, transportation, delivery, unloading,
governmental sales taxes of every kind that Seller may be required to pay discharge, storage, handling, sale and use of each product including, without
with respect to the products. Purchaser shall provide Seller, on request, with limitation, the Foreign Corrupt Practices Act and United States export control
properly completed exemption certificates for any tax from which purchaser laws; in particular, without limiting the generality of the foregoing, purchaser
claims exemption. shall not resell or ship to persons on the Denied Parties List or located within
embargoed countries (in both cases as defined under the referenced export
control laws). Purchaser further agrees to protect, defend and hold harmless
Shipping Seller from and against all claims, demands, causes of action, damages,
All orders are shipped via Seller’s recommended carriers unless an alternative
losses, liabilities, costs, expenses (including reasonable attorneys' fees),
agreement has been arranged with the purchaser. Applicable freight
penalties, and judgments (each, a "Claim") associated with the processing,
and handling charges will be added to purchaser’s invoice. Refrigerated
transportation, delivery, unloading, discharge, storage, handling, sale or use
shipments will be charged a nominal fee. Please consult a Customer Service
of any product after delivery which is (i) inconsistent with any information
Representative for confirmed delivery dates. If another carrier is specified, a
provided to purchaser or (ii) in violation of any applicable law, statute,
carrier account number must be provided by purchaser.
ordinance or regulation of any Governmental Authority. Seller assumes no
liability for failure of discharge or unloading implements or materials used
Damaged or Missing Product by purchaser whether or not supplied by Seller.
Contact a Customer Service Representative for replacement of damaged
or missing product. Damage or discrepancies must be reported within 5 Since Seller has no control over purchaser's (or others') use, disposition,
business days after receipt of product. subsequent processing, admixing or reaction of Seller’s products with other
products, chemicals or materials, purchaser assumes the entire liability
and responsibility therefor and agrees to protect, defend and hold harmless
Seller from and against all Claims associated therewith including, without
limiting the generality of the foregoing, Claims associated with infringement
of any third party's intellectual property rights, patents on processes
practiced by purchaser or patents on products made by purchaser.
v
World – Lonza Rockland, Inc. / Lonza Walkersville, Inc. / Lonza Sales Ltd
Warranty Product Use

DUE TO THE VARIOUS FACTORS AFFECTING RESEARCH TEST RESULTS, SELLER In general, all products listed in this catalog are “For Research Use Only”
WARRANTS ONLY AND NOT FOR ANY PARTICULAR PURPOSE OF THE PURCHASES, or “For Laboratory Use Only” and are not intended for use in diagnostic
THAT ALL PRODUCTS SOLD WILL PERFORM ACCORDING TO ESTABLISHED procedures unless otherwise noted. Such products are not to be used for
PRODUCT SPECIFICATIONS. PRODUCTS ARE SOLD WITH THE UNDERSTANDING diagnostic or drug purposes, or for administration to humans.
THAT THE PURCHASER WILL DETERMINE IF THE PRODUCT IS SUITABLE FOR ITS
APPLICATION. SELLER WILL REPLACE ANY PRODUCT, FREE OF CHARGE, THAT Clonetics® and Poietics® Cell products are intended for research purposes
DOES NOT MEET SELLER’S ESTABLISHED PRODUCT RELEASE SPECIFICATIONS. only and the purchaser has no rights to transfer the products, components,
or materials made using these products, or use these products for
ANY TECHNICAL ADVICE FURNISHED OR RECOMMENDATION MADE BY SELLER Commercial Purposes. Commercial Purposes include 1) use of the products
OR ANY REPRESENTATIVE THEREOF CONCERNING ANY USE OR APPLICATION OF or their components in manufacturing; 2) use of the products or their
ANY PRODUCT IS BELIEVED TO BE RELIABLE BUT SELLER MAKES NO WARRANTY, components to provide a service, information or data; 3) use of the products
EITHER EXPRESS OR IMPLIED, AS TO ITS ACCURACY OR COMPLETENESS OR OF or their components for therapeutic or diagnostic purposes; 4) resale of the
THE RESULTS TO BE OBTAINED. NO STATEMENT IS INTENDED OR SHOULD BE products or their components. Contact Marketing at Lonza Walkersville for
CONSTRUED AS A RECOMMENDATION TO INFRINGE ANY EXISTING PATENT. WITH further information.
REGARD TO ANY PROCESSING OF ANY PRODUCT, PURCHASER ASSUMES FULL
RESPONSIBILITY FOR QUALITY CONTROL, TESTING AND DETERMINATION OF For the Amaxa® brand of products the following additional terms and
SUITABILITY OF PRODUCT FOR ITS INTENDED APPLICATION OR USE. conditions apply:
SELLER MAKES NO WARRANTY OF ANY KIND, EITHER EXPRESS OR IMPLIED, Please note that Lonza´s Amaxa® Nucleofector® Technology is not
BY FACT OR LAW, OTHER THAN SELLER'S (I) OBLIGATION TO DELIVER PRODUCT intended to be used for diagnostic purposes, for testing or treatment in
COMPLYING WITH SELLER'S PUBLISHED SPECIFICATIONS (OR AS OTHERWISE humans.
REFERENCED IN THE TERMS AND CONDITIONS) AND (II) IMPLIED WARRANTIES
OF TITLE, FREEDOM FROM ENCUMBRANCE, AND RIGHT TO TRANSFER SAME. The Nucleofector® Technology, comprising Nucleofection® Process,
SELLER MAKES NO WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE, OR Nucleofector® Device, Nucleofector® Solutions, Nucleofector® 96-well
WARRANTY OF MERCHANTABILITY OTHER THAN AS STATED HEREIN. Shuttle® System, and 96-well Nucleocuvette® plates and modules is
covered by patent and/or patent pending rights owned by Lonza.
Seller guarantees the performance of Clonetics® and Poietics® Cells up to
two years from purchase only if appropriate Clonetics® or Poietics® Media Amaxa, HiFect, maxFP, maxGFP, Nucleocuvette, Nucleofection,
and Reagents are used exclusively, and the recommended storage and use Nucleofector, pmaxCloning, XpressNOW and 96-well Shuttle are either
protocols are followed. Cell and media performance is not guaranteed if any registered trademarks or trademarks of Lonza in Germany and/or the
modifications are made to such cell systems. U.S. and/or other countries.
The CMV promoter is covered under the U.S. patents 5,168,062 and
Limitation of Liability 5,385,839 and its use is permitted for research purposes only. Any other
SELLER SHALL NOT BE LIABLE FOR ANY DAMAGES OR INJURY TO PERSONS
use of the CMV promoter requires a license from the University of Iowa
OR PROPERTY ARISING FROM THE PURCHASE OR USE OF THE PRODUCT. IN
Research Foundation, 214 Technology Innovation Center, Iowa City, IA.
ADDITION, SELLER IS NOT LIABLE FOR THE PRODUCT AFTER THE PRODUCT
EXPIRATION DATE OR FOR A PRODUCT THAT HAS BEEN MISUSED OR HAS BECOME
Products contain a proprietary nucleic acid coding for a proprietary
UNUSABLE DUE TO IMPROPER STORAGE OR HANDLING BY PURCHASER.
fluorescent protein(s) intended to be used for research purposes only.
Any use of proprietary nucleic acid or fluorescent proteins coding by
SELLER'S TOTAL LIABILITY AND PURCHASER'S EXCLUSIVE REMEDY FOR ANY
proprietary nucleic acid other than for research use is strictly prohibited.
CAUSE OF ACTION ASSOCIATED WITH THE PURCHASE, WHETHER BASED IN
USE IN ANY OTHER APPLICATION REQUIRES LICENSE FROM EVROGEN. To
TORT, CONTRACT, STRICT LIABILITY OR ANY OTHER LEGAL THEORY IS EXPRESSLY
obtain such a license, please contact Evrogen atlicense@evrogen.com.
LIMITED TO REPLACEMENT OF NONCONFORMING PRODUCT OR PAYMENT IN AN
AMOUNT NOT TO EXCEED THE PURCHASE PRICE OF THE SPECIFIC PRODUCT
ATCC® and the ATCC Catalog Marks are trademarks of ATCC used under
FOR WHICH DAMAGES ARE CLAIMED, AT SELLER'S OPTION. IN NO EVENT SHALL
License.
SELLER BE LIABLE FOR ANY OTHER DAMAGES INCLUDING, WITHOUT LIMITATION,
INCIDENTAL, SPECIAL, PUNITIVE OR CONSEQUENTIAL DAMAGES.
Freedom EVO®, Freedom EVOware®, Tecan® and the Tecan logo are in
major countries a registered trademark of Tecan Group Ltd., Männedorf,
PURCHASER SHALL INSPECT THE PRODUCT SUPPLIED HEREUNDER
Switzerland.
IMMEDIATELY AFTER DELIVERY. PURCHASER'S FAILURE TO GIVE NOTICE TO
SELLER OF ANY CLAIM WITHIN FIVE (5) DAYS AFTER THE DATE OF DELIVERY
BD FACSCalibur is a trademark of Becton, Dickinson and Company.
SHALL CONSTITUTE UNQUALIFIED ACCEPTANCE OF THE PRODUCT AND A
WAIVER BY PURCHASER OF ALL CLAIMS WITH RESPECT THERETO.
Other product and company names mentioned herein are the trademarks
of their respective owners.
Resale
Products may be resold only by the Seller or its affiliate companies and
authorized distributors. A list of authorized distributors and affiliate
companies can be found in the back of this catalog or on our website,
Governing Law for orders made with Lonza Walkersville,
www.lonza.com. Inc. or Lonza Rockland, Inc.
The purchase and Terms and Conditions shall take effect and be construed
in accordance with the laws of the State of New York, USA.
Product Label
Please read the product label carefully for safety information and warnings
related to product hazards. Some products may present flammable, toxic, or Governing Law for orders made with Lonza Sales Ltd
other hazards. More information regarding certain products can be obtained The Purchase and Terms and Conditions shall take effect and be construed
by reading the MSDS, available on request from Customer Service, Scientific in accordance with the laws of Switzerland, without any reference to its
Support or on the web at www.lonza.com. The absence of a warning must conflicts of law.
not be construed as an indication that the product is safe. All possible
hazards may not be known at this time. Some of our products may contain
materials of animal or human origin. Products should only be handled and
used by qualified personnel familiar with the potential hazards and trained
in laboratory procedures. The purchaser assumes all risks of use and/or All rights reserved.
handling. Copyright Lonza 2008.
vi
Trademarks
The following are trademarks of Lonza Group or its affiliates.
Trademarks
96-well Shuttle® FastLane® maxFP® OGM™ ProEVA PAK™ SkBM®
ABM™ FBM® maxGFP® OPGM™ ProFreeze™ SkGM®
AdipoRed™ FGM® MBM® OsteoAssay™ ProHT™ StellARay™
AggreSolve™ gene transfer begins here™ MDE® OsteoImage™ ProMEDIA Select® SmBM®
AGM™ Genetic Technology Grade™ MEBM® OsteoLyse™ ProNSO™ SmGM®
Amniochrome® Global Patern Recognition™ MEGM® PC-1™ ProPer™ ToxiLight®
Amaxa® GS System™ MGM® PDELight® ProVero™1 Transport™
ApoGlow® GS Gene Expression System™ MsBM™ PGM™ PyroGene® UltraCHO™
BEBM® HBM™ MsGM™ PKLight® PYROGENT® UltraCULTURE™
BEGM® HCM™ MSCGM™ pmaxCloning™ PYROSPERSE® UltraDOMA-PF™
BioPro™ 1 HiFect® MycoAlert® PNGM™ QCL-1000® UltraDOMA™
BioWhittaker® HL-1™ MycoZap™ Poietics® ReagentPack™ UltraMEM™
BulletKit® HMM™ Nephros™ PowerCHO® REBM™ UltraMDCK™
CalciFluor™ HPGM™ NHEPS® PPiLight® REGM™ UltraPAK™
CDM™ Insect-XPRESS™ NPBM™ PrEBM™ Reliant® Versalinx®
CGM™ KBM® NPMM™ PrEGM™ SABM™ ViaLight®
Clonetics® KGM® Nucleocuvette® PrimeFect™ SAGM™ WinKQCL®
EBM® Latitude® Nucleofection® Pro293™ SCBM™ X-VIVO™
EGM® LGM-3® Nucleofector® ProCHO™ SCGM™
ExPro™ Lymphochrome™ OBM™ ProDoma™ SingleQuots®
Lonza Group or its affiliates are owner or licensee** of the following Trademarks from other companies:
patents. Products described herein may be covered by one or more ABI Prism, GeneScan, and True Allele are registered trademarks of PE Corporation,
Applied Biosystems Division
of these United States patents, by pending patent applications, or by
ALFexpress is a registered trademark of Amersham Pharmacia Biotech AB.
corresponding patents or applications in other countries.
Amplitaq, GeneAm and Fugene are registered trademarks of Roche Molecular
4,695,548** 5,219,923 6,365,341 Systems, Inc.
4,749,620* 5,641,626** 6,414,136 ATCC are trademarks of ATCC used under License.
BAX is a registered trademark of Qualicon Inc., a subsidiary of E.I. du Pont de Nemours
4,861,448** 5,836,445 6,464,850 and Co.
5,055,517 6,004,767 6,558,521 BD FACSCalibur is a registered trademark of Becton, Dickinson and Company
5,143,646 6,117,293 6,599,711 Bio-Rad, CHEF-DR, Gene Pulser, Kaleidoscope, PROTEAN, MiniPROTEAN, Ready Gel, and
Sub-Cell are registered trademarks of Bio-Rad Laboratories, Inc.
5,212,299** 6,328,870 7,166,692 Cobe is a registered trademark of Cobe Laboratories, Inc.
D510,770 D511,386 D524,449 Centipede, Millipede, and EasyCast and trademarks of Thermo Fisher Scientific, Inc.
6,521,456 7,320,859 7,332,332 C-Flex is a registered trademark of Seeman Plastics, Inc.
CrossLaps is a registered trademark of Osteometer Biotech A/S
5,168,062 5,385,839
EndoFree and siRNA are registered trademarks of Qiagen
United Kingdom patent: EndoTrap is a registered trademark of Profos, AG
2,357,336 B Eppendorf is a registered trademark of Eppendorf AG
European patents: Freedom EVO, Freedom EVOware, Tecan are registered trademarks Tecan Group, Ltd
Ficoll-Paque PLUS is a registered trademark of Amersham Bioscience/GE Helthcare
1297119 1383901 1390518 FisherBiotech is a registered trademark of Deutsche Bank, AG
1476537 1525900 1522587 Gibco, GlutaMax, Lipofectamine and Stealth are registered trademark of Invitrogen
1702677 1741778 PCT/EP01/07348 GeneSeive, StellARray and Global Pattern Recognition are trademarks of Bar Harbor
BioTechnology, Inc.
PCT/DE02/01489 PCT/DE02/01483 Hoefer is a registered trademark of Hoefer Scientific, Inc.
LI-COR is a registered trademark of LI-COR, Inc.
Matrigel is a registered trademark of Discovery Labware, Inc.
Neupogen is a registered trademark of Amgen, Inc.
Pluronic is a registered trademark of BASF Corporation
PER.C6 is a registered trademark of Crucell Holland, BV
Rainbow is a registered trademark of GE Healthcare UK
Retronectin is a registered trademark of TaKaRa Shuzo Company, LTD.
RNALater is a registered trademark of Ambion, Inc.
SeeBlue is a registered trademark of Novel Experimental Technology Corporation
SpectrMax is a registered trademark of Molecular Devices
Steady-Glo is a registered trademark of Promega
STYROFOAM is a registered trademark of Dow Chemical Corporation
SYPRO, SYBR, Alexa Fluor, Cell Tracker, Lysotracker, Mitotracker, Live/Dead, SYTO, SYTOX,
and Vybrant are registered trademarks of Molecular Probes Corporation, Inc.
Tecan, Freedom EVO and Freedom EVOware are registered trademarks of Tecan Group Ltd.
Transwell is a registered trademark of Corning, Inc.
Tween is a trademark of ICI Americas, Inc.
Versene is a registered trademark of Dow Chemical Company
Windows, Windows XP, Windows 2000 is a registered trademark of Microsoft Corporation
vii
Notes
viii
Clonetics® and Poietics®
Primary Cells and Media
Chapter 1
Clonetics® and Poietics® Primary Cells and Media
Clonetics® and Poietics® Quick Reference Guide 3 Poietics® Stem Cells & Media
Clonetics® Primary Cells & Media Introduction 81

Donor Programs 82
Introduction 7 Bone Marrow and Mononuclear Cells 83
Clonetics® Conditionally Immortalized Cell Lines 8 Hematopoietic Progenitor Cells and Media 86
Clonetics® Cell Models 9 Mesenchymal Stem Cells and Media 90
Preadipocyte Cells and Media 91
Hepatocytes and Media Osteoclast Precursor Cells and Media 92
Hepatocytes and Media Immune System Cells 93
Cells and Media
Human 14 Neural Progenitor Cells Media 97

Primary
Rat 17 Custom Cell Isolation Services 98
Human Cells & Media

New Products
Airway Epithelial Cells and Media 18
Bladder Cells and Media 21 Adult Normal Human Epidermal Melanocytes 41
Endothelial Cells and Media 23 Clonetics® Rat Cardiac Myocyte Growth Medium 72
Fibroblast Cells and Media 36 Ready Heps™ Fresh Human Hepatocytes 16
Epidermal Keratinocyte Cells and Media 40
Epidermal Melanocyte Cells and Media 45
Cell Biology Technical Information
Mammary Epithelial Cells and Media 47
Neural Cells and Media 49 Clonetics® Frequently Asked Questions 99
Prostate Cells and Media 51 Clonetics® Technical Information 101
Renal Cells and Media 54 Clonetics® Media 111
Skeletal Cells and Media 58 Poietics® Frequently Asked Questions 121
Skeletal Muscle Cells and Media 61 Poietics® Technical Information 122
Smooth Muscle Cells and Media 64 Poietics® Media 123
Animal Cells & Media

Introduction 69
Endothelial Cells and Media 69
Rat Cardiac Myocytes and Media 72
Primary Neural Cells and Media 73
Primary Neuron Growth Media 77
Cell Culture Reagents 78
Custom Cell Services 79
THESE PRODUCTS ARE FOR RESEARCH USE ONLY.

Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vivo procedures.
WARNING: CLONETICS® AND POIETICS® PRODUCTS CONTAIN HUMAN SOURCE MATERIAL, TREAT AS POTENTIALLY INFECTIOUS.
Each donor is tested and found non-reactive by an FDA approved method for the presence of HIV-1, Hepatitis B Virus, and Hepatitis C Virus.
Where donor testing is not possible, cell products are tested for the presence of viral nucleic acid from HIV-1, Hepatitis B Virus, and Hepatitis C
Virus. Testing cannot offer complete assurance that HIV-1, Hepatitis B Virus, and Hepatitis C Virus are absent. All human sourced products should be
handled at the Biological Safety Level 2 to minimize exposure of potentially infectious products, as recommended in the CDC-NIH Manual, “Biosafety in
Microbiological and Biomedical Laboratories”, 1999. If you require further information, please contact your site Safety Officer or Scientific Support.
PRODUCT WARRANTY – CULTURES HAVE A FINITE LIFESPAN IN VITRO.

Lonza guarantees the performance of its cells up to two years from purchase only if appropriate Clonetics® or Poietics® Media and Reagents are used exclusively,
and the recommended storage and use protocols are followed. Cell and media performance is not guaranteed if any modifications are made to the complete cell
system.
2
Cell Types - Quick Reference
Tissue/ Cell Type Cell Cat. No. Recommended Medium Media Cat. No. Page
Adventitial Fibroblasts
Aorta CC-7014 SCGM™ CC-3205 37
Astrocytes
Human Brain CC-2565 AGM™ CC-3186 50
C57 Mouse Brain - Mixed M-AsM-330 AGM™ CC-3186 76
CD1 Mouse Brain - Mixed M-AsM-430 AGM™ CC-3186 76
Cells and Media

Rat Cx-Hi-Cp Brain - Mixed R-AsM-530 AGM™ CC-3186 75
Rat Brain - Cortex R-CxAs-520 AGM™ CC-3186 75
Primary
Rat Brain - Hippocampus R-HiAs-521 AGM™ CC-3186 75
Rat Brain - Striatum R-CpAs-522 AGM™ CC-3186 75

Chondrocytes
Cartilage CC-2550 CGM™ CC-3216 59
Dendritic Cells
Blood CC-2701 LGM®-3 CC-3211 94
Dermal Microvascular Endothelial Cells
Blood - Adult CC-2811 EGM®-2MV CC-3202 29
Lymphatic - Adult CC-2810 EGM®-2MV CC-3202 28
Blood - Neonatal CC-2813 EGM®-2MV CC-3202 30
Lymphatic - Neonatal CC-2812 EGM®-2MV CC-3202 30
Dermal - Adult CC-2543 EGM®-2MV CC-3202 28
Dermal - Neonatal CC-2505 EGM®-2MV CC-3202 29
Dermal - Neonatal, pooled CC-2516 EGM®-2MV CC-3202 31
Endothelial Cells
Aorta CC-2535 EGM®-2 CC-3162 25
Bovine Aorta BW-6001 EGM®-MV CC-3125 70
Bovine Coronary Artery BW-6005 EGM®-MV CC-3125 71
Bovine Pulmonary Artery BW-6004 EGM®-MV CC-3125 71
Coronary Artery CC-2585 EGM®-2MV CC-3202 25
Iliac Artery CC-2545 EGM®-2MV CC-3202 26
Pulmonary Artery CC-2530 EGM®-2 CC-3162 32
Umbilical Artery CC-2520 EGM®-MV CC-3125 33
Umbilical Vein C2517A EGM®-2 CC-3162 33
Endothelial Cells, Pooled
Bovine Aorta BW-6002 EGM®-MV CC-3125 70
Umbilical Vein C2519A EGM®-2 CC-3162 34
Epithelial Cells
Airway CC-2547 SAGM™ CC-3118 19
Kidney CC-2556 REGM™ CC-3190 55
Kidney - Cortex CC-2554 REGM™ CC-3190 55
Mammary CC-2551 MEGM® CC-3150 48
Prostate CC-2555 PrEGM™ CC-3166 52
3
Continued
Epithelial Cells (with Retinoic Acid)

Bronchial / Tracheal CC-2540 BEGM® CC-3170 19
Epithelial Cells (without Retinoic Acid)
Bronchial / Tracheal CC-2541 BEGM® CC-3170 20
Fibroblasts
Dermal - Adult CC-2511 FGM®-2 CC-3132 38
Cells and Media
Dermal - Neonatal CC-2509 FGM®-2 CC-3132 38

Lung CC-2512 FGM®-2 CC-3132 39
Primary
Periodontal Ligament CC-7049 SCGM™ CC-3205 37

Heart
Rat Cardiac Myocytes R-CM-561 RCGM™ CC-4515 72
Hepatocytes
Human Liver CC-2591 HCM™ CC-3198 15
Rat Liver AC-6009 HCM™ CC-3198 17
Ready Heps™ See Catalog for additional information 16
Keratinocytes
Epidermal - Adult C2501A KGM®-2 CC-3107 41
Epidermal - Neonatal C2503A KGM®-2 CC-3107 42

Epidermal - Neonatal, pooled C2507A KGM®-2 CC-3107 43
Lung Microvascular Endothelial Cells
Blood CC-2815 EGM®-2MV CC-3202 27
Lymphatic CC-2814 EGM®-2MV CC-3202 27
Melanocytes
Neonatal CC-2504 MGM®-4 CC-3249 46
Adult CC-2586 MGM®-4 CC-3249 46
Mesangial Cells
Kidney CC-2559 MsGM™ CC-3146 56
Mesenchymal Stem Cells
Bone Marrow PT-2501 MSCGM™ PT-3001 90
Microvascular Endothelial Cells
Bladder CC-7016 EGM®-2MV CC-3202 22
Bovine Brain AC-2509 EMVB AC-3103 13
Heart CC-7030 EGM®-2MV CC-3202 31
Lung CC-2527 EGM®-2MV CC-3202 26
Uterus CC-2564 EGM®-2MV CC-3202 34
Myoblasts
Skeletal Muscle CC-2580 SkGM®-2 CC-3245 62
4
Continued
Neural Progenitor Cells

Brain PT-2599 NPMM™ CC-3209 97
Neurons
C57 Mouse Brain - Cortex M-Cx-300 PNGM™ CC-4461 76
CD1 Mouse Brain - Cortex M-Cx-400 PNGM™ CC-4461 76
Rat Brain - Cortex R-Cx-500 PNGM™ CC-4461 74
C57 Mouse Brain - Striatum M-Cp-302 PNGM™ CC-4461 76
Cells and Media

CD1 Mouse Brain - Striatum M-Cp-402 PNGM™ CC-4461 76
Primary
Rat Brain - Striatum R-Cp-502 PNGM™ CC-4461 74
Rat Brain - Hippocampus R-Hi-501 PNGM™ CC-4461 74

Rat Spinal Cord - Dorsal Root Ganglion R-Drg-505 PNGM™ CC-4461 74
Rat Brain Cerebellum - Granule Cells R-Cb-503 PNGM™-A CC-4512 74
Osteoblasts
Bone CC-2538 OGM™ CC-3207 59
Osteoclast Precursors
Bone 2T-110 OCP PT-8001 92
Proximal Tubule Epithelial Cells
Kidney CC-2553 REGM™ CC-3190 56
Skeletal Muscle Cells
Skeletal Muscle CC-2561 SkGM® CC-3160 62
Smooth Muscle Cells
Aorta CC-2571 SmGM®-2 CC-3182 65
Bladder CC-2533 SmGM®-2 CC-3182 22 & 65
Bronchial CC-2576 SmGM®-2 CC-3182 66
Coronary Artery CC-2583 SmGM®-2 CC-3182 66
Prostate CC-2587 SmGM®-2 CC-3182 53 & 67
Pulmonary Artery CC-2581 SmGM®-2 CC-3182 67
Umbilical Artery CC-2579 SmGM®-2 CC-3182 68
Uterus CC-2562 SmGM®-2 CC-3182 68
Stromal Cells
Prostate CC-2508 SCGM™ CC-3205 52
Adipose-Derived Cells
Subcutaneous Preadipocytes PT-5001 PGM™-2 PT-8002 91
Visceral Preadipocytes PT-5005 PGM™-2 PT-8002 91
5
Continued
Hematopoietic Cells
Cell Type Source Peripheral
Blood Bone Marrow Cord Blood Fetal Liver Page
Fresh Bone Marrow 1M-125 83
Mesenchymal Stem Cells PT-2501 90
CD4 T Cells
+ 2C-200 2W-200 95
CD14+ Monocytes 2W-400 93
CD19+ B Cells 2C-300 95
CD34+ Cells 2M-101 2C-101 2L-101 86 & 87
Cells and Media
CD133+ Cells 2M-102 2C-102 2L-102 87

Erythroid Progenitors 2C-250 88
Primary
Leukopak Peripheral Blood Cells 1A-125 83

Mononuclear Cells 2M-125 2C-150 CC-2702 84
Stromal Cells 2M-302 89
Products are available in various sizes. Please refer to the catalog for size information.
Hematopoietic Cell Media
Cat. No. Description Size Page
PT-3926 HPGM™ Hematopoietic Progenitor Growth Medium 500 ml 87
CC-3211 LGM®-3 Lymphocyte Growth Medium-3 500 ml 94
04-380Q X-VIVO™ 10 Chemically Defined, Serum-free Hematopoietic 1L 265
Cell Medium, with L-glutamine, gentamicin, and phenol red
Cell Medium, with L-glutamine, without gentamicin or phenol red
Cell Medium, with L-glutamine, without gentamicin or phenol red
BE04-418F X-VIVO™ 15 Chemically Defined, Serum-free Hematopoietic 500 ml 265
Cell Medium, with gentamicin, L-glutamine, and phenol red
6
Clonetics® Primary Cells and Media
In vivo relevance. In vitro results.
Cells and Media

Primary
Clonetics® Primary Cells and Media include cells that General Ordering and Shipping Information
are primarily derived from normal human tissue and are Cryopreserved cells and media products are normally shipped Monday–
Thursday for next day delivery. Saturday and Monday deliveries are
negative for HIV-1, Hepatitis B and C, and by standard available upon special request. Proliferating cell orders must be placed no
cell culture tests for mycoplasma and sterility. Immuno later than Tuesday at 5 p.m. EST for shipment the following Monday (for
delivery on Tuesday). Orders placed after Tuesday will be held until the
and special staining protocols, as well as characteristic next production cycle. When you place your order for proliferating cells,
morphology are used to characterize the cells and assure please order the appropriate media. Other cell types maybe available upon
request.
they are the designated type. A Certificate of Analysis is
available for each cell type. The cells will perform as stated Plate orders are an additional passage and take an extra week.
when used with the optimized system comprised of Cell pellet orders require 7 – 10 production days. Please plan accordingly.
Clonetics® Cells, Media and Reagents. Cells are available
Matrigel® or collagen is required for culturing hepatocytes.
cryopreserved, proliferating (in either flasks or plates),
or as pellets in RNALater®, a reagent that inactivates
RNases and stabilizes RNA within unfrozen tissues and
cells.
Clonetics® Media Products have been specially designed

to support the growth of these cells. The media systems
are comprised of serum-free basal media and SingleQuots®
Kits of growth factors and supplements. For detailed
information about these media systems, please see
pages 112 – 120.
7
Clonetics® Conditionally Immortalized Cell Lines
During recent years, increased pressure has been Characterization for Cell-based Drug Discovery
placed on drug development teams to deliver products
Lonza has qualified the conditionally immortalized cell
that meet the prescribed claims, lack side effects, and
lines for use in high throughput, cell-based functional
perform despite the patients' genetic background. To add
assays. This offers an excellent method to identify novel
to the challenge, drug discovery resourcing has not been
compounds that target insulin resistance, for example.
growing at the same rate as the external costs required to
Compounds can be rapidly identified in such screens to
validate new drugs for the market. Now, more than ever,
produce a lead series that can be integrated into drug
pharmaceutical researchers need tools that facilitate HTS
discovery pipelines.
cell screening in complex biological systems earlier in the
Immortalized Cells
process in order to eliminate poor drug candidates early As part of the characterization of our most promising
Conditionally
and allow focus on more promising candidates. conditionally immortalized human muscle cell lines, we
screened a diverse chemical library, assaying for effects
To discover a new drug, many compounds may be
on glycogen synthesis. This screen identified a novel
screened or evaluated for efficacy and toxicity. Several
series of GSK3 inhibitors.
accepted models exist (using either animals or cells), but
all have inherent limits that delay expedient evaluation
Future Products
using high throughput screening techniques. They also
require species extrapolation to evaluate the data. We are currently developing a set of conditionally
immortalized cell lines from the major insulin target tissues
Live animal models yield a rich supply of data, but
i.e. muscle, visceral and subcutaneous adipocytes.
are not cost effective for high throughput screening.
These human cell lines, optimized for diabetes research
Non-immortalized, primary cells may best represent
and high throughput cell-based assays, originate from
normal physiology, but are typically not available in the
only ethically obtained tissues.
large, uniform quantities needed for HTS applications.
Though conventionally immortalized cell lines can divide For more information concerning licensing opportunities, please call
301-898-7025, ext. 7350.
indefinitely into large homogeneous cell populations
needed for HTS applications, they do not behave like
normal cells. Conditional immortalization allows for, at Conditional Immortalization
a permissive temperature, production of high volumes
of uniform cell populations. Changing the culture 37°C – Normal Phenotype
temperature forces the cells to stop dividing, allowing Cells isolated from
for differentiation and maturation so that the cells can human tissues
express normal function and phenotype.

Transfect with
Now pharmaceutical researchers have access to a Large T-antigen
and telomerase
continuous supply of differentiated cells of the same
genotype that can be used as cell models in functional
Cells are created
cell-based assays and in long-term gene expression expressing temperature
studies. sensitive Large T-antigen
and telomerase
Available Cell Types 33°C – Large T-antigen Active Immortalization

Switched ON
Immortalized Phenotype
— Human adipocytes (gene expression altered)
— Human skeletal muscle cells

Cell population
— Human fibroblasts expanded due to
immortalization
— Human aortic smooth muscle cells
— Human endothelial cells (blood, lymph) Immortalization
37°C – Normal Phenotype Switched OFF
— Human keratinocyte cells
Cells revert to a
— Human hepatocyte cells normal phenotype
8
Clonetics® Cell Models
Corneal Epithelial Model Ordering Information

The corneal epithelium provides a transparent barrier to Cat. No. Size Price
the passage of foreign materials into the eye. Damage to Corneal Epithelial Model
this epithelial layer from environmental challenges (e.g.,
foreign substances, infection, etc.) may act to compromise CMS-2015 24 tissue models in a 24-well
plate, one empty 24-well
tissue function with respect to both barrier properties plate
and clarity. In the most severe cases, the resulting injury
may include loss of sight. The oldest and most widely Corneal Epithelial Model BulletKit®
Includes Basal Medium and SingleQuots® Kit
used method for the assessment of ocular irritants is the
CC-4444 —
Draize rabbit eye irritation test, which has been employed
Cell Models
for many years to evaluate and classify a wide range of
chemicals and cosmetic products. The Draize test involves — ET50 values established using 0.3% Triton X-100
the placement of substances directly into the conjunctival — Measurable IL-1a increases in the culture medium
sac of rabbit eyes in vivo with subsequent evaluation for relative to time of irritant exposure
irritation and damage. Ethical concerns calling for the use
— Cultures achieve stratification of 4 – 9 cell layers with
of alternative methods to animal testing and uncertainty
an average of 6 – 7 layers
regarding inter-species comparisons between rabbits
and humans have led to an increased focus on the use of — Supplied ready-to-use in Corning Transwell® Inserts
human tissue models for screening purposes. In order to
create a physiologically relevant in vitro human corneal
epithelial culture model, it is desirable that cells used ■ Product Applications
be of human origin (species-specific) and isolated from – Ocular irritancy evaluation for:
corneal epithelial tissue (tissue-specific). – Pharmaceutical products
– Pharmaceutical raw materials
Due to the difficulty in obtaining consistent high-quality – Personal care products
primary human corneal epithelial cells in sufficient – Cosmetics
numbers, the use of cell lines presents an – Standard household use chemicals
attractive alternative. However, many continuous – Basic research – corneal epithelium
cell lines lose phenotypic and/or morphological
– Ocular diseases
characteristics of the native tissue, and therefore
– Barrier function studies
do not provide a relevant system of study. We have
overcome these difficulties by the introduction ■ Routine QC Testing
of human papilloma virus 16 E6 and E7 (HPV-16 E6/E7) – Trans Epithelial Electrical Resistance – TEER is a
genes into the cells. Through the use of HPV-16 E6/E7 measure of the barrier function of epithelial cellular
in primary human corneal epithelial cells, we have junctions. The tighter the epithelial barrier, the higher
developed functional human corneal epithelial cells the TEER value, expressed as “ohms × cm2” or “Ω × cm2”.
with extended lifespan.
– ET50 (0.3% Triton X-100 as test substance)
— The Clonetics® Human Corneal Epithelial Culture (For Information Only, available upon request)
Model provides a consistent and robust platform for
References
recapitulation of human corneal epithelial tissue in an
in vitro environment 1. Draize, JH., Appraisel of the Safety of Chemicals in Foods, Drugs and
Cosmetics 1959. The Association of Food and Drug officials of the
— The tissue model achieves a histological profile United States. Austin, Texas.
2. Wilhelmus, KR., The Draize Eye Test. Survey of Ophthalmology 2001
similar to that observed with in vivo corneal epithelium May-June 45 (6):495-514.
3. Halbert, CL., Demers, GW., and Galloway, DA. The E7 Gene of Human
— The formation of epithelial junctions, essential in Papillomavirus Type 16 is sufficient for immortalization of human
establishing proper barrier function, is demonstrated epithelial cells. 1991. J. Virol 65 (1): 473-478.
4. Griffith, M., Watsky, MA., and Liu, C., Trinkaus-Randall V. Epithelial Cell
through High TEER values which reflect relevant Culture. Cornea in Methods of Tissue Engineering. ed. Atala, A., Lanza,
barrier properties and RP. 2002 Academic Press. 131-140.
5. Toropainen, E., Ranta, VP., Talvitie, A., Suhonen, P., and Urtti, A. Culture
— The human corneal epithelial culture model is predictive model of human corneal epithelium for prediction of ocular drug
absorption. Invest Ophthalmol Vis Sci. 2001 Nov 42 (12): 2942-8.
of in vivo ocular irritancy via multiple endpoints and 6. Eye Irritation: Reference Chemicals Data Bank. ECETOC Technical Report
exhibits good correlation with Draize scores No. 48, 2nd Edition. June 1998.
9
Continued
Renal Epithelial Cell Model

Clonetics® Renal Epithelial Cell Model recreates human
renal tubular function in an in vitro environment. The
model consists of primary epithelial cells isolated from
human renal cortices and seeded on extracellular-matrix-
coated polyester membrane inserts, which allow for
bicompartmental (apical and basal) exposure to culture
medium. Isolation and culture parameters (e.g., culture Renal epithelial cells stained for ZO-1 cellular
medium, matrix coating protocols, cell seeding density) junction protein
have been optimized for the development of a functional
Cell Models
renal tubular epithelial monolayer as assessed through ■ Research Applications

specific markers. – Active and passive transport studies
— Early passage normal human renal epithelial cells; – Nephrotoxicity evaluation
non-transformed, non-immortalized, primary-derived
– Renal tubular biology
— Consistent and stable expression of the apical brush
– Tight junction research
border enzyme gamma-glutamyl transpeptidase
(γ-GTP), for at least 14 days in culture – Early stage ADME-Tox screening
— Apical chamber expression of γ-GTP 10-fold higher ■ Cell Testing and Specifications
than expression in the basal chamber
– Transwell® Membrane inserts are inspected for cell
— Consistent ammoniagenesis for at least 14 days in confluence before shipping
culture
– All renal cell culture models exhibit greater than 5-fold
— Proven organic ion transport; cation uptake from the apical: basal ratio of γ-GTP
basal chamber is several times higher than the apical
chamber – 2 polyester inserts in 24-well plates seeded with renal
epithelial cells, sealed with agarose and media
— Test lots stain positive for the following cell-to-cell
junction proteins: ZO-1, e-cadherin, occludin, a-, β-, ■ Other Non-Routine Testing
γ-catenins and desmoplakin
– Ammoniagenesis
— Offered as plated cells on 12 polyester Transwell®
– Active transport of organic cations
Membranes in a 24-well plate to provide a
bi-compartmental cell environment – Barrier function
— Renal epithelial cells tested for γ-GTP expression and – Cell-to-cell junction proteins: ZO-1, e-cadherin,
apical localization occludin, a-, β-, γ-catenins and desmoplakin
— REGM™ Renal Growth Medium BulletKit®, quality tested
to support cell culture
10
Continued
g-GTP is expressed primarily on apical surface Cells Ordering Information

1,000
Renal Epithelial Cell Model
GTP molar ratio (apical:basal)
Recommended Medium: REGM™ BulletKit®
Cat. No. Size Price
100 Plated cells – polyester Transwell® Membranes

CMS-2000 12 transwell membranes
Media Ordering Information

10 REGM™ Renal Epithelial Cell Growth Medium
Cell Models
Cat. No. Size Price
REGM™ BulletKit®
1 Lot 1 Lot 2 Lot 3
CC-3190 Kit
Ratios of apical-to-basal expression of γ-GTP were averaged for three lots
of renal cortical epithelial cells in two separate experiments (triplicate wells for REBM™ Basal Medium
each lot).
CC-3191 500 ml
REGM™ SingleQuots® Supplements and Growth Factors

Basolateral uptake of organic cations CC-4127 —
8
Relative Fluorescence Units
Apical uptake
Basal uptake
0 Lot 1 Lot 2 Lot 3
Uptake of rhodamine 123 was assessed from both apical and basal culture
medium. Fluorescence of extracted rhodamine 123 was normalized to
apical concentration in each experiment. Results are from three lots of
renal cortical epithelial cells (triplicate wells for each lot) in two separate
experiments.
11
Continued
Blood Brain Barrier Model

The Clonetics® bMVEC-B Bovine Brain Microvascular
Endothelial Cell Model is a model of the “Blood Brain
Barrier”. This system is designed to significantly improve
a researcher’s ability to study active and passive transport
of drugs across the blood brain barrier, to study brain
endothelial cell tight junctions, and to study the basic
biology of brain microvascular endothelial cells. bMVEC-B culture stained for ZO-1 cellular
junction protein; nuclei counterstained with
DAPI
Brain microvascular endothelial cells form the barrier
Cell Models
between the blood circulatory system and the brain. The

barrier is formed by cellular tight junctions and active ■ Cell Testing and Specifications
transport pumps, which prevent certain substances from
entering the brain. The blood brain barrier serves to protect – Purity of cells at isolation estimated to be ≥95%
the brain from toxic substances while allowing access to – Cells are cryopreserved without growth and
essential nutrients. In this protective role, the barrier also expansion in culture (P0) at ≥750,000 per amp
functions to hinder the supply of many critical drugs to – At the recommended seeding densities, an amp of
the brain. Progress in neuropharmaceutical research cells will seed:
involves the understanding of the various systems and
proteins that are involved in blood brain barrier. – 18 wells of 24-well (2 cm2) culture plates at
approx. 20,000 cells per cm2, or
— The bMVECs express cellular tight junction proteins – one 96-well (0.32 cm2) culture plate at approx.
ZO-1 and Claudin-1 24,000 – 25,000 cells per cm2
— Cryopreserved, bMVEC-B - Bovine Brain Microvascular
bMVEC-B Cells are not intended to be serially passaged
Endothelial Cells have functional transport
in culture by customers. Cells are seeded directly,
characteristics (functional P-Glycoprotein)
without prior expansion in culture, into rat-tail collagen
— Consistent lot-to-lot performance type I coated multi-well culture plates or polycarbonate
— Purity of cryopreserved brain microvascular membranes for assays.
endothelial cells is ≥95%
■ Routine QC Testing
— Specially developed, optimized media to facilitate growth
of confluent monolayers – Cell proliferation in culture (not population doubling)
— Tested for the absence of bacterial, mycoplasmal, – DiI-acetylated LDL for endothelial cells
yeast and fungal contamination, and for viability and – Calcein AM assay for P-glycoprotein (P-gp) function,
growth after thawing using verapamil hydrochloride as a P-gp inhibitor
■ Research Applications ■ Other Non-Routine Testing

– Active or passive – Early stage – Tests for tight junction proteins ZO-1 (cytochemical
transport ADME-toxscreening staining and Western Blot), and Claudin-1 (Western
– Neurology – Pharmacology Blot)
– Tight junction research – Permeability of [14 C] sucrose across the cell

monolayer
12
Continued
Functional P-gp pump Cells Ordering Information

Fluorescence Units
Cat. No. Size Price

200
bMVEC-B - Bovine Brain Microvascular Endothelial Cells
AC-2509 ≥750,000/amp
150

100 Bovine Brain Microvascular Endothelial Cell Growth Media
Cat. No. Size Price
Cell Models
50
EMVB BulletKit®
Includes Basal Medium and SingleQuots®
AC-3103 Kit
0 0 µM 25µM
Verapamil HCI Concentration EBM®-2 Endothelial Cell Basal Medium-2
Calcein AM Efflux for P-gp Function for 3 lots of cells using
Verapamil HCl as a P-gp inhibitor. CC-3156 500 ml
EMVB SingleQuots® Supplements and Growth Factors

Low sucrose permeability AC-4207 —
12
Permeability Coefficient (cm/min x104)
10
0 Lot No. 1 Lot No. 2 Lot No. 3 Lot No. 4
Permeability of [14 C] Sucrose across Clonetics® Bovine Brain Microvascular

Endothelial Cells cultured for 10 days.
13
Hepatocytes and Media
Clonetics® NHEPS® Normal Human and Animal Primary Hepatocytes are General Ordering and Shipping Information
available cryopreserved, as fresh suspension cultures, or freshly plated in Cryopreserved cells and media products are
flasks or multiwell plates. Hepatocytes are also available as cell pellets in normally shipped Monday – Thursday for next
day delivery. Saturday and Monday deliveries
RNALater®, a reagent that inactivates RNases and stabilizes RNA within unfrozen are available upon special request.
tissues and cells. Plated hepatocytes are supplied on collagen or Matrigel®
For notification of the availability of plated
coated substrates. Other matrices and formats are offered as products through human hepatocytes, contact Customer
our Custom Laboratory. Lonza offers Clonetics® Optimized Media for the Service or your local Sales Representative.
culture of hepatocytes to provide consistent and maximum performance. All For delivery of plated hepatocytes outside the
hepatocytes are guaranteed to perform as indicated when used with Clonetics® continental United States, please contact your
local agent as to feasibility/availability.
Media and Reagents. All media lots are function-tested on the appropriate
Hepatocytes
and Media
hepatocyte cultures and performance is guaranteed.
Human hepatocytes are obtained and handled in a legal and ethically responsible
manner. Lonza’s animal facilities are AAALAC Accredited.
Human Hepatocytes
■ Performance and Quality Tests

– All human hepatocyte donors are serologically negative for HIV-1, Hepatitis B
and C, and Syphilis; all cryopreserved cells must test negative for HIV-1 and
Hepatitis B and C
– Donor information is available with each shipment
– Cryopreserved hepatocytes are tested for viability, attachment, morphology,
cytochrome P450 (phase I EROD and ECOD), uncoupled phase II activity, and Human hepatocytes
albumin expression
Human hepatocytes on Matrigel®
Human hepatocytes stained for albumin

production
14
Human Hepatocytes and Media
Continued
Cell Type Description Cryopreserved Plated Seeding Density Viable Time in Culture
h NHEPS® Human Primary Primary ≈150,000 cells/cm 2 6 to 12 hours in suspension,
hepatocytes 4 to 5 days plated
Cells Ordering Information Media Ordering Information
Cat. No. Size Price Hepatocyte Culture Media

Cryopreserved Cells Cat. No. Size Price
Hepatocytes
and Media
h NHEPS® Human
HCM™ BulletKit®
CC-2591 3 – 6 × 106 viable cells/ampoule Includes Basal Medium and SingleQuots®
CC-3198 Kit
h NHEPS® Suspension Cells (fresh)
(5 × 10 cells minimum order)
6
HBM™ Basal Medium
CC-2690 5 × 106 cells
CC-3199 500 ml
h NHEPS® Flasks – Collagen
HCM™ SingleQuots® Supplements and Growth Factors
CC-2694 T-25 flask
CC-2695 T-75 flask CC-4182 —
CC-2696 T-150 flask

HMM™ Maintenance Medium
h NHEPS® Flasks – Matrigel® CC-3197 500 ml
CC-2694B T-25 flask
HMM™ SingleQuots® Supplements and Growth Factors
CC-2695B T-75 flask
CC-2696B T-150 flask CC-4192 —
h NHEPS® Multiwell Plates – Collagen For more information about these media systems, see page 115. For
more information regarding media selection, please contact Scientific
Support at 1-800-521-0390. Matrigel® or collagen is required for culturing
CC-2691A 6 wells
hepatocytes.
CC-2692A 12 wells
Please inquire for additional formats.
CC-2693A 24 wells
CC-2698A 96 wells
h NHEPS® Multiwell Plates – Matrigel®

CC-2691B 6 wells
CC-2692B 12 wells
CC-2693B 24 wells
CC-2698B 96 wells
h NHEPS® Cell Pellet in RNALater®

PX-2591RL ≈10 million cells
15
Ready Heps™ Fresh Human Hepatocytes
Advance drug discovery research at your convenience with Clonetics® Ready

Heps™ Fresh Human Hepatocytes, available every week from Lonza. These
hepatocytes are ideal for induction studies or other toxicology experiments.
Donors are negative for HIV-1, HCV and HBV, and donor specifications are shipped
with each order.
Human hepatocytes in monolayer

Hepatocytes
Monday Tuesday Wednesday Thursday Friday

and Media
Place your order between Monday and Thursday — Week 1 References
Lonza makes the plates — Week 2–3 — For a comprehensive list of

references and additional
Replace medium and start
Receive your plates on Thursday — Week 3 experiment on Friday technical information, please
visit www.lonza.com/research
Cells Ordering Information

Cat. No. Size Price
ReadyHeps® Multiwell Plates – Collagen

Fresh Human
CC-2703W6 6 wells, (interior wells)

ReadyHeps® Flasks – Collagen

Fresh Human
CC-2703T25 T-25 flask

16
Rat Hepatocytes – Sprague-Dawley and Media
Clonetics® rt NHEPS® Rat Hepatocytes are prepared from primary isolates.

Cryopreserved rat hepatocytes are frozen without antimicrobial agents.

– Cryopreserved hepatocytes are assayed to ensure the absence of
mycoplasma, bacteria, yeast, and fungi
– Animals are barrier-raised and extensively tested by the supplier for Rat hepatocytes on collagen
serology and bacteriology, and are free of tumors and parasites
– Cryopreserved rat hepatocytes test positive for cytochrome P450 activity
Hepatocytes
and Media
(Phase 1, EROD and ECOD)
Rat hepatocytes stained for albumin production
Cell Type Description Cryopreserved Plated Seeding Density Viable Time in Culture
rt NHEPS® Hepatocytes, rat, Primary From primary 150,000 cells/cm2 7 days
Sprague-Dawley
Cat. No. Size Price Hepatocyte Culture Media
rt NHEPS®-Rat Hepatocytes Cryopreserved Cells Cat. No. Size Price
AC-6009 3 – 5 × 106 cells/ampoule HCM™ BulletKit®

rt NHEPS® Suspension Cells CC-3198 Kit
AC-2630 5 × 106 cells in 15 ml of media HBM™ Basal Medium

rt NHEPS® Flasks – Collagen CC-3199 500 ml
AC-2627A T-25 flask HCM™ SingleQuots® Supplements and Growth Factors

AC-2628A T-75 flask
CC-4182 —
rt NHEPS® Flasks – Matrigel®, pooled
HMM™ Maintenance Medium
AC-2628B T-75 flask
CC-3197 500 ml
rt NHEPS® Multiwell Plates – Collagen
HMM™ SingleQuots® Supplements and Growth Factors
AC-2621A 6 wells
AC-2622A 12 wells CC-4192 —
AC-2623A 24 wells For more information about these media systems, see page 115. For
AC-2624A 48 wells more information regarding media selection, please contact Scientific
Support at 1-800-521-0390. Matrigel® or collagen is required for culturing
AC-2625A 96 wells hepatocytes.
rt NHEPS® Multiwell Plates – Matrigel®, pooled Please inquire for additional formats.
AC-2623B 24 wells
17
Airway Epithelial Cells and Media
Airway cells are used in the functions of respiration and mucus production.
Clonetics® Human Airway Cells are available as:
— SAEC - Human Small Airway Epithelial Cells – Isolated from the distal portion
of the lung in the 1 mm bronchiole area
— NHBE - Normal Human Bronchial/Tracheal Epithelial Cells – Isolated from the
epithelial cells that line the airway above the bifurcation of the lungs
■ Research Applications NHBE - Excellent packed cuboidal morphology
– Cystic Fibrosis – Drug uptake
– Cancer – Air /Liquid interface
Cells and Media
– Respiratory disease – Asthma

– Inhalation technology
Airway
■ Cell Testing and Specifications

– We conduct rigorous lot release testing including:
– Characterization of NHBE and SAEC by morphological observation
throughout serial passage SAEC - Stained for Cytokeratin 19
– SAEC stain positive for cytokeratin 19
– Guaranteed through 15 population doublings when using Clonetics®
Media and Reagents Morphological Performance
■ Media and Supplements Criteria
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured ■ Cells rounded and not flat
in our appropriate media system ■ Cells shiny and refractile
– Use SAGM™ Small Airway Epithelial Cell Growth Medium with SAEC ■ High mitotic index
– Use BEGM® Bronchial Epithelial Cell Growth Medium with NHBE ■ No enlarged cells visible
– NHBE systems are offered with or without retinoic acid; retinoic acid
prevents squamous cell growth and differentiation
– The media systems are offered as BulletKit® Products (basal medium and
separately packaged growth factors) References
– Provides flexibility to manipulate media components specific to your — For a comprehensive list of
application references and additional
technical information, please
18
Continued
Cell Type Description Cryopreserved Proliferating Flasks Recommended Time to Subculture

Seeding Density
SAEC Small airway 1st or 2nd 2nd or 3rd passage 2,500 cells/cm2 5 to 9 days
epithelial passage
NHBE with RA Bronchial/tracheal 1st passage 2nd passage 3,500 cells/cm2 6 to 9 days
epithelial
NHBE without RA Bronchial/tracheal 1st passage 2nd passage 3,500 cells/cm2 6 to 9 days
epithelial
Cells and Media

Airway
Cells Ordering Information Cells Ordering Information
SAEC – Human Small Airway Epithelial Cells NHBE – Normal Human Bronchial/Tracheal Epithelial Cells
Recommended Medium: SAGM™ BulletKit®, see page 52 (isolated and cultured with retinoic acid)
Recommended Medium: BEGM® BulletKit®, see page 20
Cat. No. Size Price
Cat. No. Size Price
Cryopreserved Cells
Cryopreserved Cells
CC-2547 ≥500,000 cells/ampoule
SAGM™ BulletKit®
Includes Basal Medium and SingleQuots® Kit BEGM® BulletKit®
CC-3118 Kit
CC-3170 Kit
Proliferating Cells – Flasks Proliferating Cells – Flasks

CC-2647 T-25 flask CC-2640 T-25 flask
CC2547T150 T-150 flask CC2540T150 T-150 flask
Proliferating Cells – Multiwell Plates Proliferating Cells – Multiwell Plates

CC-2547W6 6 wells CC-2540W6 6 wells
CC-0094 96 wells CC-0136 96 wells
Cell Pellet in RNALater® Cell Pellet in RNALater®

PX-2547RL ≈10 million cells/pellet PX-2540RL ≈10 million cells/pellet
Ordering information continues on next page
19
Continued

NHBE – Normal Human Bronchial/Tracheal Epithelial Cells SAGM™ Small Airway Epithelial Cell Growth Media
(isolated and cultured without retinoic acid)
Recommended Medium: BEGM® BulletKit® Cat. No. Size Price
Cat. No. Size Price SAGM™ BulletKit®

Cryopreserved Cells
CC-3118 Kit
SABM™ Basal Medium
BEGM® BulletKit®
Cells and Media
Includes Basal Medium and SingleQuots® Kit CC-3119 500 ml

Airway
CC-3170 Kit SAGM™ SingleQuots® Supplements and Growth Factors

Proliferating Cells – Flasks CC-4124 —
CC-2641 T-25 flask

CC-0285 T-75 flask
BEGM® Bronchial Epithelial Cell Growth Media
CC2541T150 T-150 flask
CC2541T225 T-225 flask Cat. No. Size Price
Proliferating Cells – Multiwell Plates BEGM® BulletKit®

CC-2541W6 6 wells
CC-3170 Kit
CC-2541W12 12 wells
CC-2541W24 24 wells BEBM® Basal Medium
CC-2541W48 48 wells
CC-3171 500 ml
CC-0100 96 wells
BEGM® SingleQuots® Supplements and Growth Factors
Cell Pellet in RNALater®
CC-4175 —
PX-2541RL ≈10 million cells/pellet
ReagentPack™ Subculture Reagents
Trypsin/EDTA, Trypsin Neutralizing Solution, and HEPES Buffered Saline
Solution
CC-5034 100 ml each
For more information about these media systems, see page 112
Related Products
BSMC - Normal Human Bronchial Smooth Muscle Cells 66
HMVEC-L - Human Lung Microvascular Endothelial Cells 26
HMVEC-LLy - Human Lung Lymphatic Microvascular 27
Endothelial Cells
NHLF - Normal Human Lung Fibroblasts 39
PrimeFect™ Transfection Reagents 203
NHDF - Nucleofector® Kit 151
NHDF - 96-well Nucleofector® Kit 151
20
Bladder Cells and Media
The bladder serves as a reservoir for water soluble byproducts generated during
cell metabolism. Clonetics® Bladder Cells are available as: BdSMC - Human
Bladder Smooth Muscle Cells and HMVEC-Bd - Human Bladder Microvascular
Endothelial Cells – isolated from the surrounding bladder tissue.
■ Research Applications
– Overactive bladder
– Cancer HMVEC-Bd
– Urologic disease
Cells and Media

Bladder
– BdSMC stain positive for smooth muscle alpha actin and negative for von
Willebrand Factor
– HMVEC-Bd stain positive for von Willebrand Factor and LDL and negative for
smooth muscle alpha actin
– Guaranteed through 10 population doublings when using Clonetics® Media HMVEC-Bd
and Reagents
■ Media and Supplements
– Use SmGM®-2 Smooth Muscle Growth Medium-2 for BdSMC Morphological Performance
– Use EGM®-2MV Microvascular Endothelial Growth Medium-2 for HMVEC-Bd Criteria
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured ■ No multinucleation
in our appropriate media system ■ Cells not enlarged
Ordering information on next page ■ Minimal vacuolation
References
— For a comprehensive list of
21
Bladder Cells and Media
Continued
Cell Type Description Cryopreserved Proliferating Recommended Time to

Seeding Density Subculture
BdSMC Bladder smooth muscle 3rd passage 4th passage 3,500 cells/cm2 6 to 9 days
HMVEC-Bd Bladder microvascular 3rd passage 4th passage 5,000 cells/cm2 6 to 9 days
endothelial

BdSMC – Human Bladder Smooth Muscle Cells HMVEC-Bd – Human Bladder Microvascular Endothelial Cells
Cells and Media
Recommended Medium: SmGM®-2 BulletKit® Recommended Medium: EGM®-2MV BulletKit®

Bladder
Cat. No. Size Price Cat. No. Size Price
Cryopreserved Cells Cryopreserved Cells

CC-2533 ≥500,000 cells/ampoule CC-7016 ≥500,000 cells/ampoule
SmGM®-2 BulletKit® EGM®-2MV BulletKit®

Includes Basal Medium and SingleQuots® Kit Includes Basal Medium and SingleQuots® Kit
CC-3182 Kit CC-3202 Kit
Proliferating Cells - Flasks Proliferating Cells - Flasks

Proliferating Cells - Plates Proliferating Cells - Plates

CC-7016W6 6 wells
CC-2533W6 6 wells
CC-7016W12 12 wells
CC-2533W12 12 wells
CC-7016W24 24 wells
CC-2533W24 24 wells
CC-7016W48 48 wells
CC-2533W48 48 wells
CC-7016W96 96 wells
CC-2533W96 96 wells
Media Ordering Information Media Ordering Information

Bladder Smooth Muscle Cell Growth Media Bladder Microvascular Endothelial Cell Growth Media
SmGM®-2 BulletKit® EGM®-2MV BulletKit®

SmBM® Basal Medium EBM®-2 Basal Medium

CC-3181 500 ml CC-3156 500 ml
SmGM®-2 SingleQuots® Supplements and Growth Factors EGM®-2MV SingleQuots® Supplements and Growth Factors
CC-4149 — CC-4147 —

Related Products Solution
CC-5034 100 ml each

PrimeFect™ Transfection Reagents 204 For more information about these media systems, see pages 151, 158
22
Endothelial Cells and Media
Endothelial cells are the membrane or monolayer lining of cells taken from blood
vessel, heart, lymphatic, surface spinal cord and brain, or anterior eye chamber
of normal human tissue.
Clonetics® Endothelial Cells & Media are available from:

— Large vessels – including human aorta; umbilical artery and vein; coronary,
iliac and pulmonary artery
HCAEC culture stained for Factor VIII
— Small vessels – including dermal, lung and uterine microvascular, or other
tissues relevant to cell-specific or disease-specific research
Cells and Media

Endothelial
— Cells from single or pooled donors
— Cryopreserved lots from single donors, allow you to compare variability
among people with different characteristics
– Atherosclerosis – Inflammation
HAEC
– Angiogenesis – Wound healing
– Arteriosclerosis – Drug uptake or drug discovery
– Oncology – Cell-to-cell junctions

– Positive for acetylated low density lipoprotein uptake; normal healthy Morphological Performance
endothelial cells take in LDL and store in vacuoles Criteria
– Positive for von Willebrand Factor Expression/Factor VIII; positive stain ■ Cells refractile
reflects a healthy endothelial cell; von Willebrand Factor synthesis is critical ■ High mitotic index
to blood clotting
■ Small, similar sized cells
– PECAM-positive for lung microvascular cells; stains for adherent junctions
■ No pericytes or smooth muscle
that endothelial cells use to control passage of molecules across the tissue
cells or enlarged cells visible
– HUVEC characterized by morphological observation only
– Up to 15 population doublings guaranteed when using Clonetics® Media and
Reagents; individual cell types may vary
References
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured
in our appropriate media — For a comprehensive list of
– Use EGM® or EGM®-2 Medium Endothelial Media with large vessel cell types
– EGM®-2 Medium is a more defined medium with only 2% FBS and several visit www.lonza.com/research
growth factors replacing BBE - Bovine Brain Extract
– EGM®-MV and EGM®-2MV Media are optimized for use with microvascular
endothelial cells
separately packaged growth factors)
– Provides flexibility to manipulate media components specific to your application
23
Continued
Cell Type Description Cryopreserved Cells Proliferating Cells Recommended Time to

HAEC Aortic endothelial 3rd passage 4th passage 2,500 – 5,000 cells/cm2 5 to 9 days
HCAEC Coronary artery 3rd passage 4th passage 2,500 – 5,000 cells/cm2 5 to 9 days
HIAEC Iliac artery endothelial 3rd passage 4th passage 2,500 – 5,000 cells/cm 2 5 to 9 days
HMVEC-C Cardiac microvascular 3rd passage N/A 2,500 – 5,000 cells/cm2 5 to 9 days
HMVEC-L Lung microvascular 3rd or 4th passage 4th or 5th passage 5,000 cells/cm2 5 to 9 days
HMVEC-LBI Lung blood microvascular 3rd passage 4th or 5th passage 5,000 cells/cm2 4 to 7 days
HMVEC-LLy Lung lymphatic microvascular 3rd passage 4th passage 5,000 cells/cm 2 4 to 7 days
Cells and Media
HMVEC-dAd Adult dermal microvascular 3rd passage 4th or 5th passage 5,000 cells/cm2 5 to 9 days
Endothelial
HMVEC-dBIAd Adult dermal blood 3rd passage 4th or 5th passage 5,000 cells/cm 2 4 to 7 days
microvascular
HMVEC-dBINeo Neonatal dermal blood 3rd passage 4th or 5th passage 5,000 cells/cm2 4 to 7 days
microvascular
HMVEC-dLyAd Adult dermal lymphatic 3rd passage 4th passage 5,000 cells/cm2 4 to 7 days
microvascular
HMVEC-dNeo Neonatal dermal microvascular 3rd passage 4th or 5th passage 5,000 cells/cm2 5 to 9 days
HMVEC-dLyNeo Neonatal dermal lymphatic 3rd passage 4th passage 5,000 cells/cm2 4 to 7 days
microvascular
HPAEC Pulmonary artery 3rd passage 4th passage 2,500 – 5,000 cells/cm2 5 to 9 days
HUAEC Umbilical artery 3rd passage 4th passage 2,500 – 5,000 cells/cm2 5 to 9 days
HUVEC Umbilical vein 1st passage 2nd passage 2,500 – 5,000 cells/cm2 5 to 9 days
UtMVEC-Myo Uterine microvascular 3rd passage 4th or 5th passage 5,000 cells/cm 2 5 to 9 days
Related Products PAGE
HMVEC-Bd - Human Bladder Microvascular Endothelial Cells 22

Human Endothelial Cell Nucleofector® Kits 156-158
24
Continued

HAEC – Human Aortic Endothelial Cells HCAEC – Human Coronary Artery Endothelial Cells
Recommended Medium: EGM®-2 BulletKit®, see page 35 Recommended Medium: EGM®-2MV BulletKit®, see page 35

EGM®-2 BulletKit® EGM®-2MV BulletKit®
Cells and Media

Endothelial
Proliferating Cells – Flasks – in EGM®-2 Medium Proliferating Cells – Flasks – in EGM®-2MV Medium
Proliferating Cells – Multiwell Plates – in EGM®-2 Medium Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium

HAEC – Human Aortic Endothelial Cells HCAEC – Human Coronary Artery Endothelial Cells
Cat. No. Size Price
Cat. No. Size Price
Proliferating Cells – Flasks – in EGM® Medium
C2585AT25 T-25 flask
Proliferating Cells – Multiwell Plates – in EGM® Medium

C2585AW6 6 wells
C2535AW6 6 wells
C2585AW12 12 wells
C2535AW12 12 wells
C2585AW24 24 wells
C2535AW24 24 wells
C2585AW48 48 wells
C2535AW48 48 wells
C2585AW96 96 wells
C2535AW96 96 wells
25
Continued

HIAEC – Human Iliac Artery Endothelial Cells HMVEC-L – Human Lung Microvascular Endothelial Cells
Recommended Medium: EGM®-2MV BulletKit®, see page 35 Recommended Medium: EGM®-2MV BulletKit®, see page 35

EGM®-2MV BulletKit® EGM®-2MV BulletKit®

Cells and Media
Endothelial



26
Continued

HMVEC-LLy – Human Lung Lymphatic Microvascular HMVEC-LBl – Human Lung Blood Microvascular Endothelial
Endothelial Cells Cells

Cells and Media

Endothelial

CC-2814T25 T-25 flask CC-2815T25 T-25 flask

27
Continued

HMVEC-dAd – Adult Human Dermal Microvascular HMVEC-dLyAd – Adult Human Dermal Lymphatic
Endothelial Cells Microvascular Endothelial Cells

Cells and Media

Endothelial
Proliferating Cells – Flasks – in EGM®-2MV Medium Proliferating Cells – Flasks

CC-2643 T-25 flask CC-2810T25 T-25 flask
CC2543T150 T-150 flask CC-2810T150 T-150 flask
Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium Proliferating Cells – Multiwell Plates

HMVEC-dAd – Adult Human Dermal Microvascular

Endothelial Cells
Cat. No. Size Price
Proliferating Cells – Flasks – in EGM®-MV Medium

Proliferating Cells – Multiwell Plates – in EGM®-MV Medium

C2543AW6 6 wells
C2543AW12 12 wells
C2543AW24 24 wells
C2543AW48 48 wells
C2543AW96 96 wells
28
Endothelial Cells & Media
Continued

HMVEC-dBlAd – Adult Human Dermal Blood Microvascular HMVEC-dNeo – Neonatal Human Dermal Microvascular
Endothelial Cells Endothelial Cells, Single Donor

Cells and Media

Endothelial
Proliferating Cells – Flasks Proliferating Cells – Flasks – in EGM®-2MV Medium

CC-2811T25 T-25 flask CC-2605 T-25 flask
CC-2811T75 T-75 flask CC-0246 T-75 flask
CC-2811T150 T-150 flask CC2505T150 T-150 flask
CC-2811T225 T-225 flask CC2505T225 T-225 flask
Proliferating Cells – Multiwell Plates Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium
CC-2811W96 96 wells CC-0112 96 wells

HMVEC-dNeo – Neonatal Human Dermal Microvascular

Endothelial Cells, Single Donor
Cat. No. Size Price


C2505AW6 6 wells
C2505AW12 12 wells
C2505AW24 24 wells
C2505AW48 48 wells
C2505AW96 96 wells
29
Continued

HMVEC-dLyNeo – Neonatal Human Dermal Lymphatic HMVEC-dBl Neo – Neonatal Human Dermal Blood
Microvascular Endothelial Cells, Single Donor Microvascular Endothelial Cells

Cells and Media

Endothelial


30
Continued

HMVEC-dNeo – Neonatal Human Dermal Microvascular HMVEC-C – Human Cardiac Microvascular Endothelial Cells
Endothelial Cells, Pooled Recommended Medium: EGM®-2MV BulletKit®, see page 35
Recommended Medium: EGM®-2MV BulletKit®, see page 35
Cat. No. Size Price
Cat. No. Size Price
Cryopreserved Cells
Cryopreserved Cells
EGM®-2MV BulletKit®
Cells and Media

EGM®-2MV BulletKit® Includes Basal Medium and SingleQuots® Kit
Endothelial
CC-3202 Kit
CC-3202 Kit
Proliferating Cells – Flasks
Proliferating Cells – Flasks – in EGM®-2MV Medium
Proliferating Cells – Multiwell Plates
Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium
CC-7030W6 6 wells
CC-2516W96 96 wells

HMVEC-dNeo – Neonatal Human Dermal Microvascular

Endothelial Cells, Pooled
Cat. No. Size Price


C2516AW6 6 wells
C2516AW12 12 wells
C2516AW24 24 wells
C2516AW48 48 wells
C2516AW96 96 wells
31
Continued

HPAEC – Human Pulmonary Artery Endothelial Cells
Recommended Medium: EGM®-2 BulletKit®, see page 35 HPAEC – Human Pulmonary Artery Endothelial Cells
Cryopreserved Cells Proliferating Cells – Flasks – in EGM®-MV Medium

CC-2530 ≥500,000 cells/ampoule C2530BT25 T-25 flask
C2530BT75 T-75 flask
EGM®-2 BulletKit® C2530BT150 T-150 flask
Cells and Media

Endothelial
C2530BT225 T-225 flask

CC-3162 Kit
Proliferating Cells – Flasks – in EGM®-2 Medium
C2530BW6 6 wells
CC-2630 T-25 flask C2530BW12 12 wells
CC-0219 T-75 flask C2530BW24 24 wells
CC2530T150 T-150 flask C2530BW48 48 wells
CC2530T225 T-225 flask C2530BW96 96 wells
Proliferating Cells – Multiwell Plates – in EGM®-2 Medium

CC-2530W6 6 wells HPAEC – Human Pulmonary Artery Endothelial Cells
CC-2530W12 12 wells Cat. No. Size Price
CC-2530W24 24 wells
CC-2530W48 48 wells
CC-0128 96 wells C2530CT25 T-25 flask
C2530CT75 T-75 flask
Cell Pellet in RNALater® C2530CT150 T-150 flask
C2530CT225 T-225 flask

HPAEC – Human Pulmonary Artery Endothelial Cells C2530CW6 6 wells
Cat. No. Size Price C2530CW12 12 wells
C2530CW24 24 wells
C2530CW48 48 wells
C2530AT25 T-25 flask C2530CW96 96 wells

C2530AW6 6 wells
C2530AW12 12 wells
C2530AW24 24 wells
C2530AW48 48 wells
C2530AW96 96 wells
32
Continued

HUAEC – Human Umbilical Artery Endothelial Cells HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor
Recommended Medium: EGM®-MV BulletKit®, see page 35 Recommended Medium: EGM® BulletKit® or EGM® Complete Medium, see page 35

EGM®-MV BulletKit® EGM® BulletKit®

Cells and Media

Endothelial
CC-3124 Kit
CC-3125 Kit
Proliferating Cells – Flasks Proliferating Cells – Flasks – in EGM® Medium

CC-2617 T-25 flask
CC-2620 T-25 flask
CC-0216 T-75 flask
CC-0322 T-75 flask
Proliferating Cells – Multiwell Plates Proliferating Cells – Multiwell Plates – in EGM® Medium
CC-2517W6 6 wells
CC-2520W6 6 wells
CC-2517W12 12 wells
CC-2520W12 12 wells
CC-2517W24 24 wells
CC-2520W24 24 wells
CC-2517W48 48 wells
CC-2520W48 48 wells
CC-0124 96 wells
CC-0085 96 wells

HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor
Recommended Medium: EGM®-2 BulletKit®, see page 35 for more media
HUAEC – Human Umbilical Artery Endothelial Cells Cat. No. Size Price
Cat. No. Size Price Cryopreserved Cells

Proliferating Cells – Flasks – in EGM®-2MV Medium C2517A ≥500,000 cells/ampoule
C2520AT25 T-25 flask EGM®-2 BulletKit®

C2520AT75 T-75 flask Includes Basal Medium and SingleQuots® Kit
CC-3162 Kit
Proliferating Cells – Flasks – in EGM®-2 Medium
Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium
C2520AW6 6 wells C2517AT75 T-75 flask
C2520AW48 48 wells
C2520AW96 96 wells Proliferating Cells – Multiwell Plates – in EGM®-2 Medium
C2517AW6 6 wells
C2517AW12 12 wells
Ordering information continues on next page C2517AW24 24 wells

C2517AW48 48 wells
C2517AW96 96 wells
33
Continued

HUVEC – Human Umbilical Vein Endothelial Cells, Pooled UtMVEC-Myo – Human Uterine Microvascular Endothelial
Recommended Medium: EGM® BulletKit® or EGM® Complete Medium Cells
Cat. No. Size Price Recommended Medium: EGM®-MV BulletKit®
Cat. No. Size Price
Cryopreserved Cells
CC-2519 ≥500,000 cells/ampoule Cryopreserved Cells
EGM® BulletKit®
Cells and Media
Includes Basal Medium and SingleQuots® Kit EGM®-2MV BulletKit®

Endothelial
CC-3124 Kit Includes Basal Medium and SingleQuots® Kit

CC-3202 Kit
CC-2619 T-25 flask Proliferating Cells – Flasks – in EGM®-MV Medium
CC2519T150 T-150 flask CC-0331 T-75 flask
CC-2519W6 6 wells Proliferating Cells – Multiwell Plates – in EGM®-MV Medium
CC-0084 96 wells
PX-2519RL ≈10 million cells/pellet Cell Pellet in RNALater®
HUVEC – Human Umbilical Vein Endothelial Cells, Pooled

Recommended Medium: EGM®-2 BulletKit®
UtMVEC-Myo – Human Uterine Microvascular Endothelial
Cat. No. Size Price
Cells
Cryopreserved Cells Recommended Medium: EGM®-2MV BulletKit®
C2519A ≥500,000 cells/ampoule
Cat. No. Size Price
EGM®-2 BulletKit® Proliferating Cells – Flasks – in EGM®-2MV Medium
CC-3162 Kit
Proliferating Cells – Flasks – in EGM®-2 Medium C2564AT150 T-150 flask
C2519AT25 T-25 flask C2564AT225 T-225 flask
C2519AT150 T-150 flask Proliferating Cells – Multiwell Plates – in EGM®-2MV Medium
C2519AT225 T-225 flask C2564AW6 6 wells
C2564AW12 12 wells
Proliferating Cells – Multiwell Plates – in EGM®-2 Medium C2564AW24 24 wells
C2519AW6 6 wells C2564AW48 48 wells
C2519AW12 12 wells C2564AW96 96 wells
C2519AW24 24 wells
C2519AW48 48 wells
C2519AW96 96 wells
34

EGM®-2 BulletKit® EGM® BulletKit®

CC-3162 Kit
CC-3124 Kit
EBM®-2 Basal Medium EBM® Basal Medium
CC-3156 500 ml
CC-3121 500 ml
Cells and Media

EGM®-2 SingleQuots® Supplements and Growth Factors EBM® Basal Medium
Endothelial
Phenol Red Free
CC-4176 —
CC-3129 500 ml
EGM®-2MV BulletKit®
EGM® SingleQuots® Supplements and Growth Factors
CC-3202 Kit
CC-4133 —
EGM®-2MV SingleQuots® Supplements and Growth Factors EGM®-MV BulletKit®
CC-4147 —
CC-3125 Kit
EGM® Complete Medium
(Supplemented)
EGM®-MV SingleQuots® Supplements and Growth Factors
CC-3024 500 ml
CC-4143 —

Solution
CC-5034 100 ml each
For more information about these media systems, see page 113 –114
For more related products, see page 24
Endothelial cells must be cultured in their isolation medium for best results.
35
Fibroblast Cells and Media
Fibroblasts are found in the stroma of tissue, where they play several important
roles, such as manufacturing growth factors and protein fibers.
Clonetics® Fibroblast Cells are sourced from:

— NHDF-Ad - Adult Human Dermal Fibroblasts, from adult skin tissue
— AoAF - Human Aorta Adventitial Fibroblasts from tunica external of ascending
or descending aorta NHDF 40% confluent
— NHDF-Neo - Neonatal Human Dermal Fibroblasts, from neonatal foreskins

Cells and Media
— NHLF - Normal Human Lung Fibroblasts from adult lung tissue

Fibroblast
– Fibrosis – Fibrosarcoma – Hypertension
– Toxicology – Cell signaling – Heart disease
■ Cell Testing and Specifications NHDF-excellent, uniform morphology

– NHLF test negative for von Willebrand Factor, cytokeratins 18 and 19, and
smooth muscle alpha actin
– NHDF characterized by morphological observation throughout serial passage Morphological Performance
– AoAF stain negative for alpha actin Criteria
– HPdLF stain negative for pan cytokeratin ■ Cells elongated and fibrous
appearance
– NHLF and NHDF guaranteed through 15 population doublings when using
Clonetics® Media and Reagents ■ Refractile like smooth muscle
cells
– AoAF and HPdLF guaranteed through 10 population doublings when using
Clonetics® Media and Reagents ■ High mitotic index

– Clonetics® Cells are guaranteed to perform to our release criteria if cultured References
in our appropriate media
– Use FGM® Fibroblast Growth Medium or FGM®-2 Fibroblast Growth Medium-2 references and additional
for NHDF-Ad and NHDF-Neo technical information, please
– Use SCGM™ Stromal Cell Growth Medium for AoAF and HPdLF visit www.lonza.com/research
– FGM® is a defined, serum-free medium and FGM®-2 contains serum, which
aids in proliferation
36
Continued

AoAF Aortic adventitial 2nd passage 3rd passage 3,500 cells/cm2 6 to 9 days
fibroblasts
HPdLF Periodontal ligament 2nd passage 3rd passage 3,500 cells/cm2 6 to 9 days
fibroblasts
NHDF-Ad Adult dermal fibroblasts 1st passage 2nd passage 3,500 cells/cm2 6 to 9 days
NHDF-Neo Neonatal dermal 1st passage 2nd passage 3,500 cells/cm2 6 to 9 days
fibroblasts
Cells and Media

NHLF Lung fibroblasts 2nd passage 3rd passage 2,500 cells/cm2 6 to 9 days
Fibroblast
AoAF – Human Aortic Adventitial Fibroblasts HPdLF – Human Periodontal Ligament Fibroblasts
Recommended Medium: SCGM™ BulletKit®, see page 39 Recommended Medium: SCGM™ BulletKit®, see page 39

SCGM™ BulletKit® SCGM™ BulletKit®



37
Continued

NHDF-Ad – Human Adult Dermal Fibroblasts NHDF-Neo – Human Neonatal Dermal Fibroblasts
Recommended Medium: FGM®-2 BulletKit® Recommended Medium: FGM®-2 BulletKit®

FGM®-2 BulletKit® FGM®-2 BulletKit®

Cells and Media
Fibroblast




Basic Nucleofector® Kits for Primary Mammalian Fibroblasts 154-155
38
Continued

NHLF – Human Lung Fibroblasts Cat. No. Size Price
Recommended Medium: FGM®-2 BulletKit®
FGM®-2 BulletKit®
Cat. No. Size Price Includes Basal Medium and SingleQuots®
CC-3132 Kit
Cryopreserved Cells
CC-2512 ≥500,000 cells/ampoule FBM® Fibroblast Basal Medium
CC-3131 500 ml
FGM®-2 BulletKit®
Cells and Media

FGM®-2 SingleQuots® Supplements and Growth Factors
Fibroblast
CC-3132 Kit
CC-4126 —
FGM® BulletKit®
CC-2612 T-25 flask Includes Basal Medium and SingleQuots® Kit
CC-0282 T-75 flask CC-3130 Kit
FGM® SingleQuots® Supplements and Growth Factors
CC-4134 —
SCGM™ BulletKit®
CC-2512W6 6 wells Includes Basal Medium and SingleQuots® Kit
CC-2512W12 12 wells CC-3205 Kit
CC-2512W24 24 wells
CC-2512W48 48 wells SCBM™ Basal Medium
CC-0164 96 wells CC-3204 500 ml
Cell Pellet in RNALater® SCGM™ SingleQuots® Supplements and Growth Factors

PX-2512RL ≈10 million cells/pellet CC-4181 —

Solution
CC-5034 100 ml each
For more information about these media systems, see pages 115, 120
39
Epidermal Keratinocyte Cells and Media
Epidermal keratinocytes perform a barrier function in the skin and mouth,

protecting against invasion of bacteria and foreign particles. They also keratinize,
producing a hard collagen shell.
Clonetics® Epidermal Keratinocyte Cells are sourced from:
— Human neonatal foreskins and adult skin tissue

NHEK - 60% confluent
— NHEK-Ad - Adult Normal Human Epidermal Keratinocytes are available from
single donors
Cells and Media
Keratinocyte
— NHEK-Neo - Neonatal Normal Human Epidermal Keratinocytes are available

as single donors or pooled
– Epithelial cell model – Cancer
– Wound healing – Drug efficacy
NHEK - 100% confluent
– Burn therapy – Immunology
– Dermatology disorders
■ Cell Testing and Specifications Morphological Performance

– NHEK are characterized by morphological observation throughout serial Criteria
passage ■ Cells rounded and not flat
– Guaranteed through 15 population doublings when using Clonetics® Media ■ Cells shiny and refractile
and Reagents
■ High mitotic index
■ Media and Supplements ■ No enlarged cells visible
– Use KGM®-2 Keratinocyte Growth Medium-2 or KGM® Keratinocyte Growth References
Medium; KGM®-2 drives faster proliferation
– KGM®-CD provides a serum-free, non-animal origin, chemically defined references and additional
growth medium technical information, please
– The media systems are offered as BulletKit® Products (basal medium and visit www.lonza.com/research
– Provides flexibility to manipulate media components specific to your
application
– Media formulations are also offered without calcium and as complete media
40
Continued

NHEK-Ad Epidermal Keratinocytes, adult 1st passage 2nd passage 3,500 cells/cm2 5 to 9 days
NHEK-Neo Epidermal Keratinocytes, neonatal 1st passage 2nd passage 3,500 cells/cm2 5 to 9 days

NHEK-Ad – Adult Normal Human Epidermal Keratinocytes NHEK-Ad – Adult Normal Human Epidermal Keratinocytes
Cells and Media

Keratinocyte
Recommended Medium: KGM®-2 BulletKit®, see page 44 Recommended Medium: KGM® BulletKit® or KGM® Complete, see page 44

C2501A ≥500,000 cells/ampoule CC-2501 ≥500,000 cells/ampoule
KGM®-2 BulletKit® KGM® BulletKit®


C2501AT25 T-25 flask CC-2601 T-25 flask
C2501AT150 T-150 flask CC2501T150 T-150 flask

C2501AW6 6 wells CC-2501W6 6 wells
C2501AW96 96 wells CC-0104 96 wells

41
Continued

NHEK-Neo – Neonatal Normal Human Epidermal NHEK-Neo – Neonatal Normal Human Epidermal
Keratinocytes Keratinocytes
Recommended Medium: KGM®-2 BulletKit®, see page 44 for more media Recommended Medium: KGM® BulletKit® or KGM® Complete, see page 44

Cells and Media
Keratinocyte



C2503AW96 96 wells CC-0108 96 wells

Note: Cells isolated in KGM®-2 should only be cultured in KGM®-2. Cells isolated in KGM® can be cultured in KGM® or KGM®-2.
42
Continued

NHEK-Neo – Neonatal Normal Human Epidermal NHEK-Neo – Neonatal Normal Human Epidermal
Keratinocytes, Pooled Keratinocytes, Pooled
Recommended Medium: KGM®-2 BulletKit®, see page 44 for more media Recommended Medium: KGM® BulletKit® or KGM® Complete, see page 44

Cells and Media

Keratinocyte
CC-3107 Kit
CC-3111 Kit

CC-2607 T-25 flask
CC-0255 T-75 flask

C2507AW6 6 wells
CC-2507W6 6 wells
C2507AW12 12 wells
CC-2507W12 12 wells
C2507AW24 24 wells
CC-2507W24 24 wells
C2507AW48 48 wells
CC-2507W48 48 wells
C2507AW96 96 wells
CC-0156 96 wells

Note: Cells isolated in KGM®-2 should only be cultured in KGM®-2. Cells isolated in KGM® can be cultured in KGM® or KGM®-2.
43
Continued
KGM®-CD BulletKit® KGM® BulletKit®

CC-4455 Kit
CC-3111 Kit
KBM®-CD Keratinocyte Basal Medium, Chemically Defined KBM® Basal Medium
CC-3255 500 ml
CC-3101 500 ml
Cells and Media
KGM®-CD SingleQuots® Supplements and Growth Factors KGM® SingleQuots® Supplements and Growth Factors
Keratinocyte
CC-4456 —
CC-4131 —
KGM®-2 BulletKit® KGM® BulletKit® w/o Ca++
CC-3107 Kit
CC-3112 Kit
KBM®-2 Basal Medium KBM® Basal Medium w/o Ca++
CC-3103 500 ml
CC-3104 500 ml
KGM®-2 SingleQuots® Supplements and Growth Factors KGM® Complete System (supplemented)
CC-4152 — CC-3001 500 ml
KGM®-2 BulletKit® w/o Ca ++
CC-3108 Kitl Solution
KBM®-2 w/o Ca++ Basal Medium CC-5034 100 ml each
CC-3158 500 ml
For more information on these media systems, see page 116
Related Products
44
Epidermal Melanocyte Cells and Media
Clonetics® Normal Human Epidermal Melanocytes are neural crest derived cells
that reside in the basal stratum of the epidermis. These cells are responsible
for producing and storing melanin that, through interaction with keratinocytes,
provide pigment and UV protection to the skin. Clonetics® Melanocyte Cells are
sourced from:
— Human neonatal foreskins available as single donors
— Human adult tissue Mel-5 staining
■ Research Applications:
Cells and Media

Melanocyte
– Cell differentiation
– Pigmentation (melanogenesis)
– Cancer
– Viral-induced transformation
– Cell adhesion
L-dopa staining
■ Cell Testing and Specifications:
– Purity through immunofluorescent labeling of Mel-5 (gp75/TRP-1), most
cultures exceed 85% Mel-5 labeling
– Functional performance through the ability of 70% of the cells in a culture References
to convert L-dopa into dopa-melanin
– Morphology and proliferative capacity throughout serial passage after references and additional
recovery from cryopreservation technical information, please
– Negative test results for HIV-1, Hepatitis B and Hepatitis C visit www.lonza.com/research

– Use MGM®-4 Melanocyte Growth Medium-4 for optimal growth of the
neonatal melanocytes, adult melanocytes also require the addition of ET-3
– The media system is offered as a BulletKit® Product (basal medium and
separately packaged growth factors) to allow for flexibility with your
research applications
45
Epidermal Melanocyte Cells and Media
Continued
Competitive Analysis of Clonetics® Melanocyte Cells and Medium Media Ordering Information
250,000
Proliferation (RLU)
MGM®-4 Medium Cat. No. Size Price

Competitor
MGM®-4 BulletKit®
200,000 Includes Basal Medium and SingleQuots® Kit
CC-3249 Kit
150,000
MBM®-4 Basal Medium
100,000 CC-3250 500 ml
Cells and Media
MGM®-4 SingleQuots® Supplements and Growth Factors

Melanocyte
50,000
CC-4435 —
0 Clonetics A
®
Clonetics B
®
Clonetics C
®
Competitor
Melanocyte Lots Phosphate Buffered Saline (PBS)
Proliferation comparison of 3 unique lots of Clonetics® NHEM - Normal 17-516F 500 ml
Human Epidermal Melanocytes cultured in MGM®-4 Medium were compared
with 1 lot of the competitor’s NHEM culture in the competitor’s growth
medium. Cell proliferation was measured using Vialight® Plus (reported Trypsin/EDTA Solution
as relative luminescent units RLU), which detects ATP levels indicative of
viable cells. Each bar represents a unique lot of Clonetics® NHEM. Results of CC-5012 100 ml
2 unique MGM®-4 Medium lots were averaged with an n = 6 per replicate.
Versene® (EDTA) 0.02%
Cells Ordering Information 17-711E 100 ml
NHEM-Neo – Neonatal Normal Human Epidermal
Trypsin Neutralizing Solution (TNS)
Melanocytes
Recommended Medium: MGM®-4 BulletKit® CC-5002 100 ml
Cat. No. Size Price

ET-3 Growth Supplement
Cryopreserved Cells
CC-4510 130 µg
Proliferating Cells – Flasks For more information about this medium, see page 117
CC-2504T150 T-150 flask
CC-2504T225 T-225 flask

CC-2504W6 6 wells
CC-2504W12 12 wells
CC-2504W24 24 wells
CC-2504W48 48 wells
CC-2504W96 96 wells
NHEM-Ad – Adult Normal Human Epidermal Melanocytes

Recommended Medium: MGM®-4 BulletKit® plus ET-3 Supplement
CC-2586 ≥500,000 cells
Proliferating Cells – Flasks and Multiwell Plates Related Products

Contact Customer Service to place an order for these products Cell Culture Reagents 78
46
Mammary Epithelial Cells and Media
Clonetics® HMEC - Human Mammary Epithelial Cells are isolated from human
adult breast tissue.
■ Applications
– Breast cancer
– Cellular function and differentiation
– Physiology HMEC 95% confluent
– Toxicology
Mammary Epithelial
Cells and Media
– HMEC test positive for cytokeratins 14 and 18, and negative for cytokeratin 19
– Guaranteed through 15 population doublings when using Clonetics® Media
and Reagents

HMEC stained for cytokeratin 18
– MEGM® Mammary Epithelial Cell Growth Medium is available as a complete Morphological Performance
medium with this system; we also offer MEBM® Mammary Epithelial Cell Criteria
Basal Medium without phenol red or without sodium bicarbonate
■ Cells rounded and not flat
■ Cells shiny and refractile
■ No enlarged cells visible
Ordering information on next page
References
47
Mammary Epithelial Cells and Media
Continued

HMEC Mammary epithelial 6th or 7th passage 7th or 8th passage 2,500 cell/cm2 6 to 9 days

HMEC – Human Mammary Epithelial Cells Cat. No. Size Price
Mammary Epithelial
Recommended Medium: MEGM® BulletKit® or KGM® Complete

MEGM® BulletKit®
Cells and Media
Cat. No. Size Price Includes Basal Medium and SingleQuots® Kit
Cryopreserved Cells CC-3150 Kit
CC-2551 ≥500,000 cells/ampoule MEBM® Basal Medium

CC-3151 500 ml
MEGM® BulletKit®
CC-3150 Kit MEBM® Phenol Red-free Basal Medium
CC-3153 500 ml
CC-2651 T-25 flask
MEBM® Sodium Bicarbonate Free Basal Medium
CC-0228 T-75 flask CC-3152 500 ml
CC2551T225 T-225 flask MEGM® SingleQuots® Supplements and Growth Factors
CC-4136 —
CC-2551W6 6 wells MEGM® Complete Medium
CC-2551W12 12 wells CC-3051 500 ml
CC-2551W24 24 wells
CC-2551W48 48 wells ReagentPack™ Subculture Reagents
CC-0140 96 wells Trypsin/EDTA, Trypsin Neutralizing Solution, and HEPES Buffered Saline
Solution
Cell Pellet in RNALater® CC-5034 100 ml each
For more information about these media systems, see page117


HMEC - Nucleofector® Kit 160
HMEC - 96-well Nucleofector® Kit 161
48
Neural Cells and Media
Clonetics® Neural Cells are used to study the function of the central nervous
system. Cells are available as:
— NHA - Normal Human Astrocytes
– Neurogenesis research – Pharmacology
– Cell physiology – Parkinson’s Disease NHA stained positive for GFAP by indirect
immunofluorescence staining
– Injury – Astrocyte-mediated neurotoxicity
Cells and Media

– Alzheimer’s Disease
Neural
– NHA test positive for GFAP
– NHA guaranteed through 10 population doublings when using Clonetics® Morphological Performance
Media and Reagents Criteria
■ Media and Supplements ■ NHA generally in stellate form

– Use AGM™ Astrocyte Growth Media for NHA References
– The media systems are offered as BulletKit® Products (basal medium and — For a comprehensive list of
separately packaged growth factors) references and additional
– Provides flexibility to manipulate media components specific to your application technical information, please
49
Neural Cells and Media
Continued
Cell Type Description Cryopreserved Proliferating Recommended Time to Subculture

Seeding Density
NHA Astrocytes 1st passage 2nd passage 5,000 cells/cm2 6 to 8 days

NHA – Normal Human Astrocytes Cat. No. Size Price
Recommended Medium: AGM™ BulletKit® AGM™ BulletKit®
Cells and Media

Cat. No. Size Price
Neural
CC-3186 Kit
Cryopreserved Cells
CC-2565 ≥1,000,000 cells/ampoule ABM™ Basal Medium
CC-3187 500 ml
AGM™ BulletKit®
AGM™ SingleQuots® Supplements and Growth Factors
CC-3186 Kit
CC-4123 —
CC-2665 T-25 flask
CC-0297 T-75 flask Solution
CC2565T150 T-150 flask CC-5034 100 ml each
For more information about medium, see page 155

CC-2565W6 6 wells
CC-2565W12 12 wells
CC-2565W24 24 wells
CC-2565W48 48 wells
CC-0093 96 wells

Neural Progenitor Cells 97

Rat Neural Cells 74
Mouse Neural Cells 76
50
Prostate Cells and Media
Prostate cells provide a glandular function in the body by generating fluid

which serves several functions in reproduction. Clonetics® Prostate Cells are
available as:
— PrEC - Human Prostate Epithelial Cells
— PrSC - Human Prostate Stromal Cells
— PrSMC - Human Prostate Smooth Muscle Cells
PrEC — peroxidase stain for cytokeratin, clone
■ Research Applications 8.13
Cells and Media

– Physiology – Drug discovery
– Cancer research – Procreation research
Prostate
– PrEC test positive for cytokeratin (clone 8.13)
– PrSC test positive for vimentin and negative for pan cytokeratin
– Both types of prostate cells guaranteed through 15 population doublings
PrSC stained for vimentin
when using Clonetics® Media and Reagents
– PrSMC stain positive for alpha actin
– PrSMC are guaranteed to 10 population doublings when using Clonetics®
Media and Reagents
Morphological Performance
■ Media and Supplements Criteria
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured ■ Cells rounded and not flat
in our appropriate media ■ Cells shiny and refractile
– Use PrEGM™ Prostate Epithelial Cell Growth Medium for PrEC
– Use SCGM™ Stromal Cell Growth Medium for PrSC
■ No enlarged cells visible
– Use SmGM®-2 Smooth Muscle Growth Medium-2 for PrSMC
– Provides flexibility to manipulate media components specific to your application References
Ordering information on next page — For a comprehensive list of
51
Continued

Seeding Density
PrEC Prostate epithelial 1st or 2nd passage 2nd or 3rd passage 2,500 cells/cm2 6 to 9 days
PrSC Prostate stromal 3rd or 4th passage 4th or 5th passage 3,500 cells/cm2 6 to 9 days
PrSMC Prostate smooth 2nd or 3rd passage 3rd or 4th passage 3,500 cells/cm2 6 to 9 days
muscle
Cells and Media

Prostate
PrEC – Human Prostate Epithelial Cells PrSC – Human Prostate Stromal Cells
Recommended Medium: PrEGM™ BulletKit® Recommended Medium: SCGM™ BulletKit®

PrEGM™ BulletKit® SCGM™ BulletKit®



CC-0088 96 wells CC-2508W96 96 wells

52
Continued

PrSMC – Human Prostate Smooth Muscle Cells SCGM™ Stromal Cell Growth Media
Recommended Medium: SmGM®-2 BulletKit®
Cat. No. Size Price
Cat. No. Size Price
SCGM™ BulletKit®
Cryopreserved Cells Includes Basal Medium and SingleQuots® Kit
CC-3205 Kit
SmGM®-2 BulletKit® SCBM™ Basal Medium
Cells and Media

CC-3204 500 ml
Prostate
CC-3182 Kit
SCGM™ SingleQuots® Supplements and Growth Factors
CC-4181 —
For more information about this medium, see page 159
PrSMC Prostate Smooth Muscle Cell Media
Proliferating Cells – Multiwell Plates Cat. No. Size Price
CC-2587W6 6 wells
SmGM®-2 BulletKit®
CC-2587W12 12 wells Includes Basal Medium and SingleQuots® Kit
CC-2587W24 24 wells CC-3182 Kit
CC-2587W48 48 wells
CC-2587W96 96 wells SmBM® Basal Medium
CC-3181 500 ml
Media Ordering Information SmGM®-2 SingleQuots® Supplements and Growth Factors

PrEGM™ Prostate Epithelial Cell Growth Media CC-4149 —
Cat. No. Size Price ReagentPack™ Subculture Reagents

PrEGM™ BulletKit® Solution
CC-3166 Kit CC-5034 100 ml each

PrEBM™ Basal Medium
CC-3165 500 ml
PrEGM™ SingleQuots® Supplements and Growth Factors

CC-4177 —
Related Products
PrEC - Nucleofector® Kit 162
PrEC - 96-well Nucleofector® Kit 162
53
Renal Cells and Media
Renal cells are found in the kidneys. They eliminate waste products and modulate
electrolytes, pH and blood plasma volume. Clonetics® Human Renal Cells are
available as:
— HRE - Human Renal Epithelial Cells
— HRCE - Human Renal Cortical Epithelial Cells
— RPTEC - Human Renal Proximal Tubule Epithelial Cells
— NHMC - Normal Human Mesangial Cells RPTEC stained positive for γ-GTP
Cells and Media
– Physiology – Glomerulonephritis
Renal
– Cancer research – Phagocytosis of immune complexes

– Prostaglandin activity – Cytokine production
– Toxicology – Cellular function and differentiation
■ Cell Testing and Specifications RPTEC 100% confluent
– RPTEC test positive for γ-GTP
– NHMC test positive for fibronectin and negative for cytokeratin 19 and von
Willebrand Factor Morphological Performance
Criteria
– HRE cells stain positive for pan cytokeratin
– HRCE stain positive for cytokeratin ■ RPTEC
– Guaranteed through 15 population doublings when using Clonetics® Media – Grow as cell swirl
and Reagents – Slightly refractile
■ Media and Supplements ■ HRCE
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured – Cobblestone appearance
in our appropriate media which can surround RPTEC
and mesangial cells if these
– Use REGM™ Renal Epithelial Growth Medium for HRE, RPTEC and HRCE populations are present
– Use MsGM™ Mesangial Cell Growth Medium for NHMC ■ NHMC
– The media systems are offered as BulletKit® Products (basal medium and – Mesangial cells are large, flat
separately packaged growth factors) cells with more cytoplasm and
– Provides flexibility to manipulate media components specific to your irregular borders
application
References
54
Continued

RPTEC Proximal tubule 1st or 2nd passage 2nd or 3rd passage 2,500 cells/cm2 5 to 9 days
HRCE Cortical epithelial 1st or 2nd passage 2nd or 3rd passage 2,500 cells/cm2 5 to 9 days
HRE Renal epithelial 1st passage 2nd passage 2,500 cells/cm2 5 to 9 days
NHMC Mesangial cells 3rd passage 4th passage 3,500 cells/cm2 5 to 9 days
Cells and Media

HRCE – Human Renal Cortical Epithelial Cells HRE – Human Renal Epithelial Cells
Renal
Recommended Medium: REGM™ BulletKit®, see page 57 Recommended Medium: REGM™ BulletKit®, see page 57

REGM™ BulletKit® REGM™ BulletKit®




55
Continued

NHMC – Normal Human Mesangial Cells RPTEC – Human Renal Proximal Tubule Epithelial Cells
Recommended Medium: MsGM™ BulletKit® Recommended Medium: REGM™ BulletKit®

MsGM™ BulletKit® REGM™ BulletKit®

Cells and Media
CC-3190 Kit
CC-3146 Kit
Renal


56
Continued

REGM™ Renal Epithelial Cell Growth Media MsGM™ Mesangial Cell Growth Media
REGM™ BulletKit® MsGM™ BulletKit®

REBM™ Basal Medium MsBM™ Basal Medium
Cells and Media

CC-3191 500 ml CC-3147 500 ml
Renal
REGM™ SingleQuots® Supplements and Growth Factors MsGM™ SingleQuots® Supplements and Growth Factors
CC-4127 — CC-4146 —

Solution
CC-5034 100 ml each
Related Products
57
Skeletal Cells and Media
Skeletal cells provide primary structural support as bone. Osteoblasts produce

bone matrix and prime it for mineralization. Chondrocytes produce and maintain
extracellular cartilage matrix. Cartilage provides joint cushioning and facilitates
joint articulation. Clonetics® Human Skeletal Cells are available as:
— NHOst - Normal Human Osteoblasts
— NHAC-kn - Normal Human Articular Chondrocytes from the knee
NHOst bone mineralization
– Physiology – Bone repair – Joint degeneration
Cells and Media
– Bone formation – Bone disease – Joint replacement

Skeletal

– NHAC-kn test positive for type II collagen and sulfated proteoglycans after
differentiation
– NHAC-kn guaranteed through 15 population doublings
NHAC-kn undifferentiated 100% confluent
– NHOst test positive for alkaline phosphatase and bone mineralization
(Von Kossa stain)
– NHOst guaranteed through 10 population doublings when using Clonetics® Morphological Performance
■ Media and Supplements ■ Osteoblasts
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured – Spindle morphology when
in our appropriate media confluent
– Use OGM™ Osteoblast Growth Medium and OGM™ Differentiation SingleQuots® – Healthy osteoblasts mineralize
Kit to expand and differentiate NHOst under differentiation conditions
– Use CGM™ Chondrocyte Growth Medium and CDM™ Chondrocyte ■ Chondrocytes
Differentiation Medium to expand and differentiate NHAC-kn – Spindle becoming mostly
– We supply sodium alginate, calcium chloride, sodium chloride, and sodium polygonal when confluent
citrate, required reagents for culture and differentiation of our chondrocytes
separately packaged growth factors) References
58
Continued

Seeding Density
NHOst Osteoblasts 2nd or 3rd passage 3rd or 4th passage 5,000 cells/cm2 6 to 9 days
NHAC-kn Articular 2nd passage 3rd passage 10,000 cells/cm2 4 to 9 days
chondrocytes, knee
Cells and Media

NHOst – Normal Human Osteoblasts NHAC-kn – Normal Human Articular Chondrocytes, Knee
Skeletal
Recommended Medium: OGM™ BulletKit®, see page 60 Recommended Medium: CGM™ BulletKit® for Growth, CDM™ BulletKit® for
Differentiation, see page 60
Cat. No. Size Price
Cat. No. Size Price
Cryopreserved Cells
Cryopreserved Cells
OGM™ BulletKit®
Includes Basal Medium and SingleQuots® Kit CGM™ BulletKit®
CC-3207 Kit
CC-3216 Kit
CDM™ BulletKit®
CC-2538T25 T-25 flask Includes Basal Medium and SingleQuots® Kit
CC-2538T75 T-75 flask CC-3225 Kit
CC2538T225 T-225 flask Proliferating Cells – Flasks
CC-2538W6 6 wells CC2550T150 T-150 flask
CC-2538W12 12 wells CC2550T225 T-225 flask
CC-2538W24 24 wells
CC-2538W48 48 wells Proliferating Cells – Multiwell Plates
CC-2538W96 96 wells
CC-2550W6 6 wells
CC-2550W12 12 wells
CC-2550W24 24 wells
PX-2538RL ≈10 million cells/pellet CC-2550W48 48 wells
CC-2550W96 96 wells

59

OGM™ Osteoblast Growth Media Chondrocyte Differentiation Media
OGM™ BulletKit® CDM™ BulletKit®
OBM™ Basal Medium CDM™ Basal Medium

Cells and Media
CC-3208 500 ml CC-3226 500 ml

Skeletal
OGM™ SingleQuots® Supplements and Growth Factors CDM™ SingleQuots® Supplements and Growth Factors
CC-4193 — CC-4408 —
OGM™ Differentiation SingleQuots® Kit

To promote differentiation
Additional reagents required to culture chondrocytes:
CC-4194 —
Cat. No. Size Price
ReagentPack™ Subculture Reagents Sodium Alginate

Solution CC-3234 50 ml
CC-5034 100 ml each
CaCl2 Solution
CC-3235 500 ml
CGM™ Chondrocyte Growth Media NaCl Solution
Cat. No. Size Price CC-3236 500 ml
CGM™ BulletKit® Sodium Citrate Solution

CC-3237 100 ml
CC-3216 Kit
Ascorbic Acid
CBM Basal Medium
CC-4398 0.5 ml
CC-3217 500 ml $63
Chondrocyte ReagentPack™ Subculture Reagents
CGM™ SingleQuots® Supplements and Growth Factors Trypsin/EDTA, Trypsin Neutralizing Solution, and HEPES Buffered Saline
Solution
CC-4409 —
CC-3233 100 ml
hMSC - Human Mesenchymal Stem Cells 90

OCP - Human Osteoclast Precursors 92
OsteoImage™ Mineralization Assay 240
60
Skeletal Muscle Cells and Media
Skeletal muscle cells form the muscles that coordinate with the skeletal system
of the body, where their contractions result in bodily movement. Clonetics®
Skeletal Muscle Cells are offered as:
— SkMC - Human Skeletal Muscle Cells are isolated as satellite cells, from the
upper arm or upper leg
— HSMM - Human Skeletal Muscle Myoblasts are isolated from post-gestational
tissue, usually from quadriceps or psoas tissue SkMC stained for Desmin
Cells and Media

Skeletal Muscle
– Gene expression – Receptor mediated function
– Differentiation – Neuromuscular disease research
– Ion transport – Diabetes
– SkMC test positive for desmin
– SkMC guaranteed through 15 population doublings when using Clonetics® Differentiated skeletal muscle (HSMM)
Media and Reagents culture stained positive for Desmin
– HSMM can be differentiated to form multinucleate myotubes in the presence
of serum-poor media or if allowed to approach 70% confluence
– HSMM test positive for desmin as myoblasts and myotubes
– HSMM guaranteed through 10 population doublings when using Clonetics® Morphological Performance
■ Media and Supplements ■ High mitotic index
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured ■ Cells fuse to form multinucleate
in our appropriate media myotubes at higher confluence
– Use SkGM® Skeletal Muscle Cell Growth Medium for SkMC
– Use SkGM®-2 Skeletal Muscle Cell Growth Medium for HSMM
References
Ordering information on next page
61
Continued

Seeding Density
HSMM Muscle myoblasts 2nd passage 3rd passage 3,500 cells/cm2 5 to 9 days
SkMC Skeletal muscle 2nd passage 3rd passage 3,500 cells/cm2 6 to 10 days

HSMM – Human Skeletal Muscle Myoblasts SkMC – Human Skeletal Muscle Cells
Cells and Media
Skeletal Muscle
Recommended Medium: SkGM®-2 BulletKit® Recommended Medium: SkGM® BulletKit®

SkGM®-2 BulletKit® SkGM® BulletKit®

without L-glutamine
CC-3160 Kit
CC-3245 Kit
CC-2661 T-25 flask
CC-0231 T-75 flask

CC-2561W6 6 wells
CC-2580W6 6 wells
CC-2561W12 12 wells
CC-2580W12 12 wells
CC-2561W24 24 wells
CC-2580W24 24 wells
CC-2561W48 48 wells
CC-2580W48 48 wells
CC-0144 96 wells
CC-2580W96 96 wells


62
Continued

SkGM® Skeletal Muscle Cell Growth Media SkGM® Skeletal Muscle Cell Growth Media
SkGM® BulletKit® SkGM®-2 BulletKit®

without L-glutamine
CC-3160 Kit
CC-3245 Kit
SkBM® Basal Medium
SkBM®-2 Basal Medium
Cells and Media

Skeletal Muscle
CC-3161 500 ml
CC-3246 500 ml
SkGM® SingleQuots® Supplements and Growth Factors
SkGM®-2 SingleQuots® Supplements and Growth Factors
CC-4139 —
CC-3244 —
DPBS
17-512F 500 ml

Solution
CC-5034 100 ml each
63
Smooth Muscle Cells and Media
Smooth muscle cells are found in many parts of the body, where they work
by controlling the movement of blood, food or air. Clonetics® Smooth Muscle Cells
are obtained from:
— Aorta — Pulmonary artery
— Major bronchia — Umbilical artery
— Coronary artery — Uterine wall
UtSMC stained for smooth muscle a-actin
Cells and Media
Smooth Muscle
– Asthma – Cystic Fibrosis

– Respiratory distress syndrome – Basic research
– Drug uptake studies – Angiogenesis
– Atherosclerosis – Vascular pathology
– Cardiovascular pharmaceutical development
■ Cell Testing and Specifications AoSMC 50% confluent
– Stain positive for a-actin and negative for von Willebrand Factor after
differentiation Morphological Performance
– Guaranteed through 15 population doublings when using Clonetics® Media Criteria
and Reagents
■ Refractile but less so than
■ Media and Supplements epithelial cells
– Clonetics® Cells are guaranteed to perform to our release criteria if cultured ■ Elongated and sometimes
in our appropriate media triangular in shape
– Use SmGM®-2 Smooth Muscle Growth Medium–2 with 5% FBS ■ High mitotic index
– The media systems are offered as BulletKit® Products (basal medium and ■ No cytoskeleton visible
– Provides flexibility to manipulate media components specific to your
application References
Ordering information on next page — For a comprehensive list of

64
Continued

Seeding Density
AoSMC Aortic smooth muscle 3rd passage 4th or 5th passage 3,500 cells/cm2 6 to 10 days
BdSMC Bladder smooth muscle 3rd passage 4th passage 3,500 cells/cm2 6 to 9 days
BSMC Bronchial smooth muscle 3rd passage 4th or 5th passage 3,500 cells/cm2 6 to 10 days
CASMC Coronary artery 3rd passage 4th or 5th passage 3,500 cells/cm2 6 to 10 days
PASMC Pulmonary artery 3rd passage 4th or 5th passage 3,500 cells/cm2 6 to 10 days
PrSMC Prostate smooth muscle 2nd or 3rd 3rd or 4th passage 3,500 cells/cm2 6 to 9 days
passage
Cells and Media

Smooth Muscle
UASMC Umbilical artery 3rd passage 4th or 5th passage 3,500 cells/cm2 6 to 10 days
UtSMC Uterine smooth muscle 3rd passage 4th or 5th passage 3,500 cells/cm2 6 to 10 days

AoSMC – Human Aortic Smooth Muscle Cells BdSMC – Human Bladder Smooth Muscle Cells
Recommended Medium: SmGM®-2 BulletKit® Recommended Medium: SmGM®-2 BulletKit®

SmGM®-2 BulletKit® SmGM®-2 BulletKit®

CC-3182 Kit
CC-3182 Kit
Proliferating Cells – Flasks Proliferating Cells - Flasks
Proliferating Cells - Plates

CC-2533W6 6 wells
CC-2571W6 6 wells
CC-2533W12 12 wells
CC-2571W12 12 wells
CC-2533W24 24 wells
CC-2571W24 24 wells
CC-2533W48 48 wells
CC-2571W48 48 wells
CC-2533W96 96 wells
CC-0148 96 wells
Bladder Smooth Muscle Cell Growth Media
Cat. No. Size Price
CC-3182 Kit
SmBM® Basal Medium

CC-3181 500 ml
SmGM®-2 SingleQuots® Supplements and Growth Factors

CC-4149 —
65
Continued

BSMC – Normal Human Bronchial Smooth Muscle Cells CASMC – Human Coronary Artery Smooth Muscle Cells
Recommended Medium: SmGM®-2 BulletKit®, see page 65 for more media Recommended Medium: SmGM®-2 BulletKit®, see page 65 for more media

Cells and Media
Smooth Muscle


CC-2576W6 6 wells
CC-2583W6 6 wells
CC-2576W12 12 wells
CC-2583W12 12 wells
CC-2576W24 24 wells
CC-2583W24 24 wells
CC-2576W48 48 wells
CC-2583W48 48 wells
CC-0180 96 wells
CC-0096 96 wells

Related Products
Smooth Muscle Cell Nucleofector® Kits 172 - 173
66
Continued

PASMC – Human Pulmonary Artery Smooth Muscle Cells PrSMC – Human Prostate Smooth Muscle Cells
Recommended Medium: SmGM®-2 BulletKit®, see page 65 Recommended Medium: SmGM®-2 BulletKit®, see page 65

Cells and Media

Smooth Muscle



PrEGM™ Prostate Epithelial Cell Growth Media
Cat. No. Size Price
PrEGM™ BulletKit®
CC-3166 Kit
PrEBM™ Basal Medium

CC-3165 500 ml
PrEGM™ SingleQuots® Supplements and Growth Factors

CC-4177 —
67
Continued

UASMC – Human Umbilical Artery Smooth Muscle Cells UtSMC – Human Uterine Smooth Muscle Cells
Recommended Medium: SmGM®-2 BulletKit® Recommended Medium: SmGM®-2 BulletKit®


Cells and Media
Smooth Muscle




Cat. No. Size Price
Includes Basal Medium and SingleQuots®
CC-3182 Kit
SmBM® Basal Medium

CC-3181 500 ml
SmGM®-2 SingleQuots® Supplements and Growth Factors

CC-4149 —

Solution
CC-5034 100 ml each Related Products PAGE
For more information about this medium, see page 120 BdSMC - Human Bladder Smooth Muscle Cells 65
PrSMC - Human Prostate Smooth Muscle Cells 85
68
Animal Cells and Media
Clonetics® Animal Primary Cells are provided with the same quality standards General Ordering and Shipping Information
as the Clonetics® Human Cell products. All cells are performance tested and test Cryopreserved cells and media products are
negative for mycoplasma, bacteria, yeast and fungi. Clonetics® Cells are guaranteed normally shipped Monday – Thursday for next
day delivery. Saturday and Monday deliveries
to perform as indicated when used with Clonetics® Cells, Media and Reagents. are available upon special request.
Immuno and special staining protocols, as well as characteristic morphology,
Proliferating cell orders must be placed no
are used to characterize the cells and assure they are the designated type. A later than Tuesday at 5 p.m. for delivery the
Certificate of Analysis is available for each cell type. Most cells are available following Monday. Orders placed after Tuesday
will be held until the next production cycle.
cryopreserved or proliferating, in either flasks for plates, or as a cell pellet in As you place your order for proliferating cells,
RNALater®, a reagent that inactivates RNases and stabilizes RNA within unfrozen please order the appropriate media.
Cells and Media

tissues and cells. The following animal cells are available: Cell pellet orders require 7 – 10 production
Endothelial
days. Please plan accordingly.
— Bovine Endothelial Cells
— Rat Cardiac Myocytes
— Rat and Mouse Neurons
■ Clonetics® Animal Endothelial Cells are available:

– bAEC–Bovine Aortic Endothelial Cells
– bCAEC–Bovine Coronary Artery Endothelial Cells
– bPAEC–Bovine Pulmonary Artery Endothelial Cells

bAEC stained for acetylated LDL
– Cells are assayed to ensure the absence of mycoplasma, bacteria, yeast,
and fungi
– Evaluated for one passage out of cryopreservation
bAEC ≈ 100% confluent
69
Continued
Cell Type Description Cryopreserved Proliferating Recommended Seeding Time to Subculture

Density
bAEC Bovine aorta 1st passage 2nd passage 2,500 – 5,000 cells/cm2 5 to 9 days
bPAEC Bovine pulmonary artery 1st passage 2nd passage 2,500 – 5,000 cells/cm2 5 to 9 days
bCAEC Bovine coronary artery 3rd passage 4th passage 2,500 – 5,000 cells/cm2 5 to 9 days

Cells and Media
bAEC – Bovine Aortic Endothelial Cells, Single Donor bAEC – Bovine Aortic Endothelial Cells, Pooled
Endothelial
Recommended Medium: EGM® MV BulletKit®, see page 31 Recommended Medium: EGM® MV BulletKit®, see page 31

BW-6001 ≥500,000 cells/ampoule BW-6002 ≥500,000 cells/ampoule
EGM®-MV BulletKit® EGM®-MV BulletKit®


AC-6001T25 T-25 flask AC-6002T25 T-25 flask

AC-6001W96 96 wells AC-6002W96 96 wells

PA-6001RL ≈10 million cells/pellet PA-6002RL ≈10 million cells/pellet
70
Continued

bCAEC – Bovine Coronary Artery Endothelial Cells bPAEC – Bovine Pulmonary Artery Endothelial Cells
Recommended Medium: EGM®-MV BulletKit®, see page 31 Recommended Medium: EGM®-MV BulletKit®, see page 31

BW-6005 ≥500,000 cells/ampoule BW-6004 ≥500,000 cells/ampoule
EGM®-MV BulletKit® EGM®-MV BulletKit®
Cells and Media

Endothelial
CC-3125 Kit $113 CC-3125 Kit


AC-6005W96 96 wells AC-6004W96 96 wells

PA-6005RL ≈10 million cells/pellet PA-6004RL ≈10 million cells/pellet
71
Rat Cardiac Myocytes and Media
Clonetics® Cryopreserved Neonatal Ventricular Rat Cardiac Myocytes (P1-3) are

high quality primary myocyte cells prepared by standardized methods, and
are ready for immediate culture upon thaw. Each vial of cardiac myocyte cells
contains approximately 4 million viable cells at ≥85% purity. When thawed
and cultured you will obtain ≥80% viability, with excellent morphology and
connectivity, and cells will display beating at 24 hours in culture.
Each lot of these cells test positive for functional syncytium formation, and stain
Rat Cardiac Myocytes
Rat Cardiac Myocytes

positive for actinin. Cells also test negative for mycoplasma and bacteria. Primary Cryopreserved neonatal rat cardiomyocytes
cardiac myocyte cells need an appropriate substrate to adhere and survive. The thawed and cultured for 13 days. Actinin – green;
gap junctions – red; nuclei – blue.
and Media
preferred substrate is nitrocellulose.
Lonza now offers fully optimized cardiac myocyte medium and growth factors
to complement Clonetics® Rat Cardiac Myocytes as a complete system to study
cardiac biology or toxicology. Clonetics® RCGM Rat Cardiac Myocyte Growth
Medium is specifically formulated for the growth and survival of rat neonatal
cardiac myocytes in culture.
Rat Cardiac Myocytes RCGM BulletKit®

R-CM-561 ≥4 million cells CC-4515 Kit
(1 ml suspension)
RCBM Basal Medium

CC-3275 200 ml
RCGM SingleQuots® Supplement and Growth Factors

CC-4516 Kit
72
Primary Neural Cells and Media
Enabling – A breakthrough technology now exists for the preparation and

cryopreservation of mammalian neuronal cells for use as primary cell cultures
for basic discovery, development studies, or screening applications. Frozen
primary neuronal cells revolutionize cell culture research because they can be
simply thawed and cultured on demand to obtain high quality and high yield
cultures of dissociated primary neurons.
Convenient – You can avoid today’s inefficiencies to conduct neuronal research Cortical and striatal rat neurons
Cells were thawed, co-cultured 21 days,
Primary Neural Cells

on living cells. Shipped overnight to your laboratory, these high quality, and immunofluorescently stained with anti-
cryopreserved, dissociated primary cells represent a cost effective way to vesicular GABA transporter (vGAT) and anti-
dopamine receptor protein (DARPP).
do neuronal primary cell culture, eliminating costly and time consuming
animal care requirements and allowing you to control the experimental/assay
timetable. Perform your primary cell culture with greater speed, convenience and
reproducibility.
Reproducible – Freshly dissociated or cultured primary neuronal cells do

not survive shipping. However, cryopreserved neuronal cells can be shipped
anywhere and used any time. Specific lots can now be archived, so researchers
can re-visit past experiments – on the same batch of cells. This can’t be done
any other way.
■ Applications
– Transfection
– Evaluation of electrophysiological properties, neurotransmitters, receptor
function
– Research typical inhibitory or excitatory ion-channels
– Receptor signaling research
– Intracellular transport studies
– Neurotoxicity research
73
Clonetics® Cryopreserved Neurons from rat brain are cell suspensions of high
quality primary embryonic brain neuronal cells (including glia) prepared by
standardized methods and are ready for immediate culture.
Each vial of neuronal cells is guaranteed to be mycoplasma and bacteria free.

Additional molecular and immunochemical testing for quality is done following
conditions that mimic shipping. Rat cortical neuronal cells
Cells were thawed, cultured 14 days, and
immunofluorescently stained with anti-PGP
Prior to cryopreservation, each vial (1 ml) of cortical and striatal neurons contain 9.5 and anti β-tubulin.
Cells and Media
Primary Neural
approximately 4 million viable cells. Each vial (0.25 ml) of hippocampus neurons
contain approximately 1 million viable cells.
Cell Type Description Cryopreserved Proliferating Culture Time

R-Cx Rat brain cortex neurons Immediate N/A 14 – 21 days
R-Hi Rat brain hippocampus neurons Immediate N/A 14 – 21 days
R-Cp Rat brain striatum neurons Immediate N/A 14 – 21 days
R-Drg Rat dorsal root ganglion neurons Immediate N/A 14 – 21 days
R-Cb Rat cerebellar neurons Immediate N/A 14 – 21 days

Rat Brain Cortex Neurons PNGM™ BulletKit®

Recommended Medium: PNGM™ BulletKit®, see page 110 Includes Basal Medium and SingleQuots® Kit
CC-4461 Kit
R-Cx-500 4 million cells per vial
Rat Brain Hippocampus Neurons PNGM™-A BulletKit®

Recommended Medium: PNGM™ BulletKit®, see page110 Includes Basal Medium and SingleQuots® Kit
R-Hi-501 1 million cells per vial CC-4512 Kit
Rat Brain Striatum Neurons

Recommended Medium: PNGM™ BulletKit®, see page 110
R-Cp-502 4 million cells per vial
Rat Dorsal Root Ganglion Neurons

Recommended Medium: PNGM™ BulletKit®, see page 110
R-Drg-505 200,000 cells per vial
Rat Cerebellar Neurons (Granule Cells)

Recommended Medium: Contact Scientific Support
R-Cb-503 4 million cells per vial
74
Continued
Clonetics® Ready-to-use Astrocytes are obtained from rat brain, passaged once,
and prepared as cell suspensions for shipment on dry ice. Each vial (1 ml)
contains approximately 1 million cells. These astrocytes can be simply thawed and
cultured. Following confluence, the astrocytes can be harvested for re-plating or
stored frozen for later use.
Each vial of astrocytes is guaranteed mycoplasma and bacteria free. Astrocytes

are batch-tested for growth characteristics and morphology (GFAP). Rat cortical cells
Immunofluorescence image of cryopreserved
rat cortical cells thawed and cultured 21
days stained with anti-PGP 9.5 and anti-
Cells and Media

Primary Neural
neurofilament.

R-CxAs Rat brain cortex astrocytes Primary passage N/A 14 – 21 days
R-HiAs Rat brain hippocampus astrocytes Primary passage N/A 14 – 21 days
R-CpAs Rat brain striatum astrocytes Primary passage N/A 14 – 21 days
R-AsM Rat brain Cx-Hi-Cp mix astrocytes Primary passage N/A 14 – 21 days

Cat. No. Size Price
AGM™ Astrocyte Growth Medium
Rat Brain Cortex Astrocytes
Recommended Medium: AGM™ BulletKit®, see page 110 Cat. No. Size Price
R-CxAs-520 1 million cells per vial AGM™ BulletKit®

Rat Brain Hippocampus Astrocytes CC-3186 Kit
Recommended Medium: AGM™ BulletKit®, see page 110
R-HiAs-521 1 million cells per vial
Rat Brain Striatum Astrocytes

R-CpAs-522 1 million cells per vial
Rat Brain Cx-Hi-Cp Mix Astrocytes

R-AsM-530 1 million cells per vial
75
Continued
Clonetics® Primary Mouse Neurons and Astrocytes include a variety of neurons

from two different mouse strains, C57 Black, and CD1. These cryopreserved
neurons are cell suspensions of high quality primary embryonic brain neuronal
cells (including glia) prepared by standardized methods, and are ready for
immediate culture. Cryopreserved mouse astrocytes are a mixed population
isolated from the hippocampus, cortex, and striatum of the brain. These
astrocytes are passaged once and prepared as cell suspensions that can be
simply thawed and cultured. Mouse cortical neuronal cells
Cryopreserved mouse cortical neuronal cells
were thawed and cultured 12 days, then
Cells and Media
immunofluorescently stained with anti-PGP

Primary Neural
9.5 and anti-β-tubulin.

M-Cx Mouse brain cortex neurons Immediate N/A 14 – 21 days
M-Cp Mouse brain striatum neurons Immediate N/A 14 – 21 days
M-AsM Mouse brain mixed astrocytes Primary passage N/A 21+ days
Cat. No. Size Price PNGM™ Primary Neuron Growth Medium

Mouse CD1 Brain Cortex Neurons Cat. No. Size Price
Recommended Medium: PNGM™ BulletKit®
M-Cx-400 4 million cells per vial
PNGM™ BulletKit®
Mouse CD1 Brain Striatum Neurons CC-4461 Kit

AGM™ Astrocyte Growth Medium
M-Cp-402 4 million cells per vial
Cat. No. Size Price
Mouse C57 Brain Cortex Neurons AGM™ BulletKit®
Recommended Medium: PNGM™ BulletKit® Includes Basal Medium and SingleQuots® Kit
M-Cx-300 4 million cells per vial
CC-3186 Kit
Mouse C57 Brain Striatum Neurons

M-Cp-302 4 million cells per vial
Mouse CD1 Brain Mixed Astrocytes

Recommended Medium: AGM™ BulletKit®
M-AsM-430 1 million cells per vial
Mouse CD57 Brain Mixed Astrocytes

Recommended Medium: AGM™ BulletKit®
M-AsM-330 1 million cells per vial
76
Primary Neuron Growth Media
Optimized medium for culturing primary neurons
Clonetics® PNGM™ Primary Neuron Growth Medium is a Media Ordering Information

serum-free medium allowing for long-term maintenance
PNGM™-A Primary Neuron Growth Media - Adult
of embryonic neuronal cells. Viability and morphology of
cultured neurons can be maintained for several weeks Cat. No. Size Price
with minimal growth of glial cells.
PNGM™-A Primary Neuron Growth Medium - Adult BulletKit®
PNGM™ Medium is conveniently packaged in our BulletKit®
CC-4512 Kit
Format containing PNGM™ Basal Medium and PNGM™
SingleQuots® Kit. The PNGM™ SingleQuots® Kit contains the PNBM™ Primary Neuron Basal Medium
Primary Neuron
necessary volumes of L-glutamine, Penicillin/Streptomycin,
Growth Media
and NSF-1 — a supplement supporting neuronal growth CC-3256 500 ml
and survival — to complement the basal medium volume

PNGM™-A Primary Neuron Growth Medium - Adult
provided. This is a complete media kit that contains all of
SingleQuots® Kit
the necessary components to support neuronal growth
and survival. CC-4511 Kit
For more information about this Medium, see page 118.
PNGM™ Primary Neuron Growth Media

Cat. No. Size Price
NEW! Clonetics® PNGM™-A Primary Neuron Growth
PNGM™ BulletKit®
Medium-Adult is a fully optimized, complete medium Includes Basal Medium and SingleQuots® Kit
specifically for culturing rat cerebellar neurons (Granule
CC-4461 Kit
cells), with the potential to support the growth of other
primary adult animal neurons. This new formulation
PNBM™ Basal Medium
addresses the unique requirements of primary adult
neurons and demonstrates exceptional growth and CC-3256 200 ml
survival for these neural cells in culture.
PNGM™ SingleQuots® Supplement and Growth Factors
PNGM™ Medium is tested and guaranteed to perform with CC-4462 —
all of our primary embryonic rat and mouse neurons, thus
For more information about this Medium, see page 118.
eliminating the risk of using unqualified material.
AGM™ Astrocyte Growth Media
Cat. No. Size Price
AGM™ BulletKit®
CC-3186 Kit
ABM™ Basal Medium

CC-3187 500 ml
AGM™ SingleQuots® Supplements and Growth Factors

CC-4123 —

Solution
CC-5034 100 ml each
For more information about this medium, see page117.
77
The following products are cell culture tested using human primary cells.
Ordering Information Ordering Information
Cat. No. Size Price Growth Factors

Trypsin Neutralizing Solution Cat. No. Size Price
CC-5002 100 ml NSF-1 Neural Survivor Factor-1
Trypsin/EDTA CC-4323 4.0 ml
CC-5012 100 ml hEGF 3 µg/ml Epidermal Growth Factor

HEPES Buffered Saline Solution CC-4107 0.5 ml
CC-5022 100 ml Bovine Pituitary Extract (BPE) 13 mg/ml

CC-5024 500 ml
CC-4009 2.0 ml
Trypsin/EDTA, Trypsin Neutralizing Solution, and HEPES Buffered Saline Bovine Brain Extract (BBE) 9 mg/ml
Solution
CC-4098 5.0 ml
CC-5034 100 ml each
hFGF 1 µg/ml Human Fibroblastic Growth Factor
CC-4068 1.0 ml
Retronectin® Recombinant Human Fibronectin Fragment
Recombinant human fibronectin fragment CH-296 produced in E. coli. When Human Transferrin 10 mg/ml
coated on the surface of flasks and plates, Retronectin significantly enhances
retrovirus-mediated gene transfer into mammalian cells. Lyophilized. CC-4205 0.5 ml
■ Storage Conditions -20°C Calcium Chloride 300 mM

CC-4202 2.0 ml
T100A 0.5 mg
Ascorbic Acid 25.5 mg/ml
T100B 2.5 mg
CC-4398 0.5 ml
Retronectin Dish
Retronectin pre-coated 35 mm dishes
T110A 10 dishes (35 mm)
Additional Cell Culture Reagents can be found on pages 284 - 285
78
Custom Cell Services
Custom Cell Isolations and Cell Culture Services Customized Options

Lonza provides a variety of Custom Cell Isolation and — Primary cell isolations from human and animal tissues
Cell Culture Services that can be designed around your
specific research needs. Primary cells are routinely — Cell expansion and testing
isolated from human and non-human tissues including — Donor-matched cell sets
endothelial, epithelial, muscle, and cardiac tissue. Other — Cryopreserved or proliferating formats
Cell Culture Services include routine delivery of cells in
special packaging formats, including multiwell plates for — Wide array of QC and cell characterization services,
Custom Cell Services

toxicity screening and flasks for expansion. For specific including PCR
inquiries and pricing, please call (800) 521-3090 to — Individual customer consultation to ensure correct
contact Scientific Support for more information. order fulfillment
Lonza has a state-of-the-art custom cell isolation laboratory
Flexible Packaging that can provide tailor-made cells not commonly available
from commercial sources. Equipped with some of the
— Variable cell concentrations
world's best cell culture technicians (with over 60 years
— Cell pellets of cell culture experience among them), we offer cell
— Choice of proliferating or cryopreserved cells cultures that can be provided on a regular basis to fit your
research schedule.
— Pre-seeded multiwell plates and flasks
— Choice of culture matrix With extensive sources for human and animal tissue,
bone marrow and peripheral blood, we can provide most
— Customer-specified cell “treatments” of the cell types that you require.
Quality Assurance ■ We offer convenience

■ We procure the tissue
— Donor serology or PCR testing of cell products for
viruses ■ We isolate the cells
— Cell characterization including functional testing ■ We deliver the cells with medium and culture reagents
and staining of your choice
— Cell performance testing including growth and ■ We take care of details, you get results
differentiation
— ISO 9001:2000 Certified quality management
company
— cGMP compliant manufacturing available upon
request
79
Notes
80
Poietics® Stem Cells
We do the isolation, you do the research
Poietics® Stem Cells and Media include a collection General Ordering and Shipping Information
of human stem and progenitor cells derived from Differentiating cells are generally available in both fresh and cryopreserved
hematopoietic, adipose, and neural systems. Fresh or formats. Orders for fresh cells are usually scheduled 1 – 2 weeks
ahead of your anticipated use date. Fresh cells are processed on the
cryopreserved cells are available from bone marrow, same day as drawn and shipped for overnight delivery. Fresh and
cord blood, and fetal liver. Media systems are available for cryopreserved cells are shipped Monday through Thursday. Fresh cells are
shipped in HBSS with 5 mM EDTA and 0.5% BSA.
the cell types. All cells are obtained through Institutional
Poietics® Stem Cells

Review Board-approved donor programs.
We also offer a number of highly purified standard cell

types as well as custom cell isolation services. All products
are quality controlled and are provided with a certificate
of analysis.
Poietics® Stem Cells and Media

Introduction 81
Donor Programs 82
Bone Marrow and Mononuclear Cells 83
Hematopoietic Progenitor Cells and Media 86
Mesenchymal Stem Cells and Media 90
Preadipocyte Cells and Media 91
Osteoclast Precursor Cells and Media 92
Immune System Cells and Media 93
Neural Progenitor Cells Media 97
Custom Cell Isolation Services 98
Cell Biology Technical Information

Clonetics® Frequently Asked Questions 99
Clonetics® Technical Information 101
Clonetics® Media 111
Poietics® Frequently Asked Questions 121
Poietics® Technical Information 122
Poietics® Media 123
81
Donor Programs
Bone Marrow ■ Normal Donor Testing

Human bone marrow products are available in both fresh – Complete blood count
and cryopreserved formats. Bone marrow is collected on
a routine basis from human donors. Normal donor eligibility – Hepatitis B surface antigen
criteria include healthy males and non-pregnant females – Hepatitis C antibody
between the ages of 18 and 45 years old. The donors must – HIV-1 antibody
have a negative medical history for all major diseases.
Donors may only donate a maximum of 6 donations total,
with informed consent and screening for each donation.
Clinical-grade bone marrow aspirates are also available.

Donors are extensively screened according to blood and
tissue bank regulations. When you are ready to move
your research into the therapeutic arena, please contact
Scientific Support for more information.
Umbilical Cord Blood Program ■ Normal Donor Testing

We offer a variety of cryopreserved cells from umbilical – Hepatitis B surface antigen
Donor Programs
cord blood. Cord blood samples are collected at the time

of delivery from both Caesarean and natural births and are – HIV-1 antibody
transported to our nearby cell laboratory for immediate
processing. Cell products derived from umbilical cord
blood are then shipped overnight to customers.
Donor screening includes general good health, uncom-

plicated delivery, healthy baby and negative test results
for Hepatitis B surface antigen and HIV-1 antibody.
82
Bone Marrow and Mononuclear Cells
Unprocessed Human Bone Marrow
Unprocessed bone marrow orders are shipped Monday Cells Ordering Information
through Thursday and are shipped the same day the bone
Cat. No. Size Price
marrow is drawn for delivery on the following morning.
Please contact your Scientific Support Representative for Unprocessed Human Bone Marrow
(Fresh)
further details on ordering fresh marrow.
1M-105 10 ml
Our high quality human bone marrow is guaranteed to 1M-125 25 ml
contain a minimum of 15 million leukocytes per ml. Order 4 × 1M-125 100 ml
(same donor)
Normal Human Peripheral Blood Leukopaks
Mononuclear Cells
Bone Marrow and
Poietics® Normal Human Peripheral Blood Leukopak is Cells Ordering Information
a bag of fresh human blood cells collected from normal
Cat. No. Size Price
peripheral blood by automated apheresis in ACD-A
anticoagulant and density gradient centrifugation. Each Normal Human Peripheral Blood Leukopak
leukopak contains a mixture of monocytes, lymphocytes, 1A-125 100 ml
platelets, plasma, and red cells. Leukopaks offer large
quantities of cells from a single donor which can be useful
in research for isolation of novel cell types, applications of
novel isolation methods, and the various other applications
associated with blood and immune cell research.
■ Features
– Monocyte enriched
– Standard leukopaks have a volume up to 100 ml and a
total white cell count ≥60 million per ml
– NOTE: Smaller volumes available upon request
– All donors are screened for general health, and test
negative for HIV-1, Hepatitis B & C
– A product information sheet is provided with each
leukopak
– PMN-enriched leukopaks available upon request
– Leukopaks with special donor characteristics are
available, such as CMV negative and EBV
83
Human Mononuclear Cells
Mononuclear cells are prepared by centrifugation in Cells Ordering Information

a density cell separation medium (Ficoll-Paque®, GE Cat. No. Size Price
HealthCare Bio-Sciences AB) from bone marrow or
cord blood. Isolating the mononuclear cells eliminates Human Bone Marrow Mononuclear Cells
(fresh)
erythrocytes and granulocytes, producing a more stable
cell product. Mononuclear cells can be used directly in 1M-125C 25 million cells
hematopoietic assays, or as the starting material for 1M-125D 100 million cells
isolating CD34+ progenitor cells. Cells are available fresh or 1M-125A 200 million cells
cryopreserved. When shipped fresh, cells are suspended 1M-125E 300 million cells
in HBSS containing 0.5% BSA and 5 mM EDTA.
Human Bone Marrow Mononuclear Cells
(cryopreserved)
Human Bone Marrow Mononuclear Cells
2M-125C 25 million cells
Bone marrow is the primary source of hematopoietic 2M-125D 100 million cells
progenitors. These progenitors can be readily isolated from 2M-125A 200 million cells
the mononuclear cell fraction. Bone marrow mononuclear
Mononuclear Cells
Bone Marrow and
cells are available from a large and varied donor pool. Human Bone Marrow Mononuclear Cell Panel
(cryopreserved, 5 donors)
2M-125B 25 million cells/donor
Human Cord Blood Mononuclear Cells
Hematopoietic progenitors exist in higher quantities in
umbilical cord blood than adult peripheral blood. Due to
fewer histocompatibility problems and greater access to Cells Ordering Information
ethnic diversity, cord blood cells are of great interest in
Cat. No. Size Price
transplantation and ex vivo expansion research. Cells are
available cryopreserved. Human Cord Blood Mononuclear Cells
(cryopreserved)
■ Media Recommendations 2C-150A 100 million cells

2C-150 200 million cells
— For serum-free media, use HPGM™ Hematopoietic
Progenitor Growth Medium plus a variety of cytokines
to support growth. To promote growth with a serum-
containing medium, use IMDM (Cat. No. 12-726 or
12-915), 15% FBS, plus cytokines. Contact Scientific
Support for additional media recommendations.
84
Quadratox 4 Species Panel of Bone Marrow Mononuclear Cells
The Quadratox 4 Species Panel is a panel of bone marrow- Cells Ordering Information
derived mononuclear cells from four different mammalian
Cat. No. Size Price
species including inbred C57BL/6 mice, purebred beagles,
baboons, and humans. Animals and tissues are obtained Quadratox 4 Species Panel
(cryopreserved)
from AAALAC-accredited facilities.
2S-101 ≥5 million cells/species
Quadratox Panels can be used to assess the relative
toxicities of chemotherapeutic drugs in pre-clinical Human Bone Marrow
(cryopreserved)
research. Inter-species differences in the relative toxicities of
2S-101D ≥5 million cells
numerous chemotherapeutic drugs are well documented.
In vitro toxicity data have been shown to contribute to Murine Bone Marrow
both the structuring and subsequent interpretation of (cryopreserved)
clinical trials. Quadratox Panels may be used for selection 2S-101C ≥5 million cells
of the most appropriate animal models for both efficacy
and toxicology studies and prediction of in vivo human Canine Bone Marrow
(cryopreserved)
Mononuclear Cells
Bone Marrow and
myelotoxicity.
2S-101B ≥5 million cells
The Quadratox 4 Species Panel is available as cryo-
Baboon Bone Marrow
preserved vials of ≥5 million cells/species. Bone marrow (cryopreserved)
mononuclear cells from human, murine, canine, and
2S-101A ≥5 million cells
baboon are also available individually.
References
— For a comprehensive list of references and additional
technical information, please visit www.lonza.com/
research
85
Hematopoietic Progenitor Cells and Media
Human CD34+ Progenitor Cells
The cell surface glycoprotein CD34 is a known marker Cells Ordering Information
for hematopoietic progenitor cells. As these cells mature
Human Bone Marrow CD34+ Progenitor Cells
and differentiate, CD34 expression is lost. CD34+ cells CD34+ Progenitor Cell purity is ≥95%.
are capable of initiating long-term hematopoiesis both in
vitro and in vivo. CD34+ Progenitor Cells are available from Cat. No. Size Price
bone marrow, cord blood, and fetal liver. They are isolated Human Bone Marrow CD34+ Progenitor Cells
from mononuclear cells using positive immunomagnetic (fresh)
selection. Analysis by flow cytometry is included. 1M-101 ≥ 100,000 cells
1M-101A ≥ 300,000 cells
Fresh cells need to be scheduled 1 – 4 weeks in advance 1M-101B ≥ 500,000 cells
depending on the source and volume requirements. 1M-101C ≥ 1,000,000 cells
Human Bone Marrow CD34+ Progenitor Cells

Erythropoiesis from CD34+ Progenitor Cells (cryopreserved)
Progenitor Cells & Media
RFU
4.0E+05
2M-101 ≥ 100,000 cells
Hematopoietic
2M-101A ≥ 300,000 cells

2M-101B ≥ 500,000 cells
2M-101C ≥ 1,000,000 cells
2.0E+05 2M-101D ≥ 2,000,000 cells
Larger quantities of Human Bone Marrow CD34+ Progenitor

25ng/ml SCF
10ng/ml SCF Cells are also available:
Please contact your technical sales representative to place one of the
0.0E+00 60 500 10,000 following custom packaging orders:
Erythropoietin
(mUnits/ml) 2M-101C ≥ 5 million cells
≥ 10 million cells
■ Media recommendations ≥ 20 million cells
— For serum-free media, use HPGM™ Hematopoietic ≥ 40 million cells
Progenitor Growth Medium plus a variety of cytokines
to support growth. To promote growth with a serum-
containing medium, use IMDM (Cat. No. 12-726 or
12-915), 15% FBS plus cytokines
86

Continued

Human Cord Blood CD34+ Progenitor Cells Human Fetal Liver CD34+ Progenitor Cells
CD34+ Progenitor Cell purity is ≥90%. Maternal blood is screened for Hepatitis B and C and HIV-1.
CD34+ Progenitor Cell purity is ≥90%.
Cat. No. Size Price
Cat. No. Size Price
Human Cord Blood CD34+ Progenitor Cells
(cryopreserved) Human Fetal Liver CD34 Progenitor Cells
+
(cryopreserved)
2C-101B ≥100,000 cells
2L-101B ≥100,000 cells
2C-101A ≥500,000 cells
2L-101A ≥500,000 cells
2C-101 ≥1,000,000 cells
HPGM™ Hematopoietic Progenitor Growth Medium Medium Ordering Information

HPGM™ Hematopoietic Progenitor Growth Medium is a Progenitor Cell Medium

serum-free medium containing only human proteins
Cat. No. Size Price
that support the proliferation of human hematopoietic
Hematopoietic
progenitor cells (CD34+ cells), with cytokines. HPGM™ Hematopoietic Progenitor Growth Medium
CD34+ cells can be stimulated to undergo a limited PT-3926 500 ml
amount of proliferation and still maintain a relatively
undifferentiated phenotype when grown in this medium.
Additionally, HPGM™ can be used to support the growth
and/or differentiation of other hematopoietic progenitor
cells such as erythroid progenitors, CD133+ cells, and
mononuclear cells.
CD133 is selectively expressed on CD34+ bright cells and Cells Ordering Information
is a subset of hematopoietic progenitor cells. CD133+ cells
Cat. No. Size Price
are isolated from human bone marrow, umbilical cord
blood, and fetal liver by positive immunoselection. Human Bone Marrow CD133+ Progenitor Cells
(cryopreserved)
References 2M-102A ≥100,000 cells

2M-102 ≥500,000 cells
technical information, please visit www.lonza.com/ Human Cord Blood CD133+ Progenitor Cells
(cryopreserved)
research
2C-102A ≥100,000 cells
NOTE: Delivery times may vary depending upon donor availability. 2C-102 ≥500,000 cells
Human Fetal Liver CD133+ Progenitor Cells

(cryopreserved)
2L-102A ≥100,000 cells

2L-102 ≥500,000 cells
87
Erythroid Progenitor Cells
Committed human erythroid progenitors are defined Cells Ordering Information

by the cell surface glycoprotein CD36, an early marker
Cat. No. Size Price
of the erythroid lineage. During the differentiation of
erythrocytes, expression of this cell surface glycoprotein Human Cord Blood Erythroid Progenitors
(cryopreserved)
precedes glycophorin A and hemoglobin alpha expression.
2C-250 ≥1 million cells
Highly purified CD34+ cells are put into culture for 7 – 8
days, in a cocktail of cytokines, excluding EPO, to stimulate
expansion. After this culture period, CD36+ Erythroid
Progenitors are isolated using positive immunoselection
and cryopreserved. Purity is ≥85%. Upon thawing, these
cells are put into culture with a cytokine cocktail including
EPO. Maturation of the erythroid progenitors occurs over
7 days in culture, with little to no differentiation to other
lineages.
Hematopoietic
Glycophorin A expression of Human Erythroid Progenitor

Cells in culture
90
% cells glycophorin A-positive
80
70
60
50
40
30
20
10
0 Day 0 Day 4 Day 7

CD36+ Cells
References
research
88
Human Stromal Cells
When bone marrow cells are put into culture under specific Cells Ordering Information
conditions, a stromal layer of adherent cells develops over
Cat. No. Size Price
a few weeks. The stromal layer is composed of many cell
types, including fibroblasts, mesenchymal stem cells, Human Bone Marrow Non-Irradiated Stromal Cells
(cryopreserved)
adipocytes, endothelial cells and macrophages. These
stromal cells function as a feeder layer for hematopoietic 2M-302 ≥5 million cells
progenitors, allowing proliferation and differentiation of
progenitors to continue for weeks in these cultures with
no addition of exogenous cytokines. This long-term culture
system allows researchers to study an in vitro model of
the bone marrow microenvironment, including cell-cell
interactions, adhesion molecules and cytokine secretion.
These and many other factors allow for the tight regulation
of blood cell production, progenitor cell commitment and

differentiation, and stem cell renewal.
Hematopoietic
References
research
89
Human Mesenchymal Stem Cells and Media
Poietics® hMSC Mesenchymal Stem Cells are harvested Cells Ordering Information
and cultured from normal human bone marrow. Cell
Cat. No. Size Price
purity is far higher than cells from stromal cell cultures.
Cells are tested for purity by flow cytometry and for their hMSC Human Mesenchymal Stem Cells
(cryopreserved)
ability to differentiate into osteogenic, chondrogenic,
and adipogenic lineages. Cells are positive for CD105, PT-2501 ≥750,000 cells
CD166, CD29, and CD44. Cells test negative for CD14,
CD34, and CD45. Media systems are available to support Media Ordering Information
growth of hMSCs and their differentiation into adipogenic, Mesenchymal Media
chondrogenic, and osteogenic lineages.
Cat. No. Size Price
References MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit®
Includes basal medium and SingleQuots® Kit
technical information, please visit www.lonza.com/ PT-3001 Kit
research MSCGM™ Mesenchymal Stem Cell Basal Medium
Mesenchymal stem cells are licensed by Lonza from Osiris Therapeutics, PT-3238 440 ml
Inc. and are subject to the following limited use license:
The included biological material, including progeny and derivatives, MSCGM™ Mesenchymal Stem Cell Growth Medium
(collectively referred to as “Material”) is licensed to you under specific
Stem Cells and Media
terms. You are responsible for ensuring that the terms of the license SingleQuots® Kit
agreement are met.
Mesenchymal
PT-4105 —
1. Grants of License: Lonza Walkersville, Inc. grants you a nontransferable,
nonexclusive license to use the Material for research.
2. Not for Human Use: The Material may not be used: a) in humans; b) in hMSC Mesenchymal Stem Cell Chondrogenic Differentiation
conjunction with human clinical trials; c) in association with human BulletKit®
diagnostics. Includes Basal Medium and SingleQuots® Kit
3. Material Not Transferable: You may not transfer the Material to any other
person or organization. PT-3003 Kit
4. Patent Notice: Material under license from Osiris Therapeutics, Inc. NOTE: TGF-β3 component sold separately
Material is covered by US patent 5,486,359 and others.
1,000
rhTGF Beta 3 for hMSC Chondrogenic Differentiation Media
1,000
Forward Scatter
Supplement
800 800
PT-4124 2 µg
600 600
400 400 hMSC Mesenchymal Stem Cell Osteogenic Differentiation

200 200 BulletKit®
0 0 200 400 600 800 1,000 0 0 200 400 600 800 1000
Side Scatter Side Scatter
PT-3002 Kit
Stromal cell culture Poietics® Human MSC
hMSC Mesenchymal Stem Cell Adipogenic Differentiation
BulletKit®
PT-3004 Kit
*Refer to page 91 to see our recomended alternative media for adipocyte
differentiation media
Subculturing Reagents
DPBS
hMSC in culture
17-512F 500 ml
Related Products Trypsin/EDTA

Osteoblast and Chondrocyte Cells and Media 59 - 60 CC-3232 100 ml
Human MSC Nucleofection® Kit 178
90
Human Preadipocyte Cells and Media
Poietics® Preadipocytes Cells are isolated from Cells Ordering Information

subcutaneous or visceral fat. Subcutaneous fat is often
Cat. No. Size Price
found attached to skin in the lower abdomen area. Visceral
preadipocytes are isolated from adipose tissue associated Human Visceral Preadipocyte Cells
(cryopreserved)
with internal organs, such as the bladder or kidney.
Relative to subcutaneous fat, visceral fat deposits are PT-5005 ≥1 million cells
mobilized at a higher rate to produce serum fatty acids Human Subcutaneous Preadipocyte Cells
which contribute to insulin resistance, Type II Diabetes, (cryopreserved)
and other related cardiovascular disorders. PT-5020 ≥1 million cells
Poietics® Preadipocyte Cells and Media contain: PT-5001 ≥4 million cells
— Cryopreserved Normal Human Preadipocyte Cells Media Ordering Information
isolated from subcutaneous or visceral fat
Preadipocyte Media
— PGM™-2 Preadipocyte Growth Medium-2 BulletKit®,
which contains the basal medium and growth Cat. No. Size Price
supplements needed to induce growth and PGM™-2 Preadipocyte Growth Medium-2 BulletKit®
differentiation of the preadipocytes into mature Includes basal medium and SingleQuots® Kit
adipocytes
PT-8002 Kit
— AdipoRed™ Assay Reagent, a new assay reagent for
high-throughput quantification of intracellular lipid PGM™-2 Preadipocyte Basal Medium
Cells and Media

Preadipocyte
Preadipocytes are precursor cells that develop into PT-8202 500 ml
adipocytes when fully differentiated. Adipocytes perform PGM™-2 Growth Medium SingleQuots® Kit
essential functions of energy metabolism and are PT-9502 Kit
characterized by the accumulation of intracellular
triglycerides. This cell system has been designed for AdipoRed™ Assay Reagent
use in high throughput applications to conduct research
PT-7009 5 × 4 ml
on lipid accumulation and metabolism, obesity, insulin
sensitivity, diabetes, and diet drugs. Subculturing Reagents
Poietics® Cells, Media, and Reagents are quality tested DPBS
together and guaranteed to give optimum performance 17-512F 500 ml
as a complete cell system. NOTE: Please inquire about availability of subcutaneous and visceral
preadipocyte sets from same donor.
Catecholamine-induced Lipolysis in Subcutaneous and

Visceral Primary Human Preadipocytes
2.5
Total Stored Triglyceride
Lipolysis (Glycerol)
2
Differentiated subcutaneous preadipocyte cells
1.5
0.5
Differentiated visceral preadipocyte cells 0 Subcutaneous Visceral
91
Human Osteoclast Precursor Cells and Media
Poietics® Osteoclast Precursor Cells and Media contain: Cells Ordering Information
— Cryopreserved Human Osteoclast Precursors Cat. No. Size Price
— OCP Osteoclast Precursor Medium BulletKit®, which Human Osteoclast Precursors
includes the basal medium and supplements needed (cryopreserved)
to induce the osteoclast precursors to differentiate into 2T-110 ≥1 million cells/amp
mature osteoclasts; these differentiated osteoclasts
stain positive for TRAP and express the calcitonin Media Ordering Information
receptor
OCP Osteoclast Precursor Media
Osteoclasts are large, multinucleated cells that play an
active role in bone resorption. This cell system has been Cat. No. Size Price
designed for use in high throughput applications to OCP Osteoclast Medium BulletKit®
conduct research on osteoporosis, bone resorption, and Includes basal medium and SingleQuots® Kit
other bone-related diseases. PT-8001 Kit
Poietics® Cells, Media, and Reagents are quality tested OCP Osteoclast Precursor Basal Medium
together and guaranteed to give optimum performance PT-8201 100 ml
as a complete cell system.
OCP Osteoclast Precursor SingleQuots® Kit
Osteoclast Precursor
PT-9501 Kit
Cells and Media
For related cells and media systems, see page 58 – 60.

For related assays using these cells, see pages 239 – 240.
Effects of calcitonin on osteoclast-mediated bone matrix

degradation in vitro
250,000
OsteoLyse (RFU)
Differentiated human osteoclasts resorbing bone fragment

200,000
150,000
100,000
50,000
Pits formed from bone resorption activity of differentiated osteoclasts 0 Control Day 5 - 6 Days 0 - 6
Calcitonin (1nM) Exposure
Inhibition of bone matrix resorption by calcitonin. Poietics® Primary Human
Osteoclast Precursors were cultured in differentiation medium containing no
calcitonin, calcitonin added only at day 5 and calcitoinin added on days 0
and 5 and assayed after a total of 6 days. Calcitonin, added at day 0, resulted
in the osteoclasts becoming refractory to calcitonin added on day 5.
Related Products
OsteoAssay™ Human Bone Plate 237
OsteoLyse™ Assay Kit (Human Collagen) 238
Calcifluor™ Quantitative Bone Resorption Assay 239
92
Human Immune System Cells and Media
Human CD14+ Monocytes
Normal human monocytes are found in the circulating Cells Ordering Information
peripheral blood; they play an important role in host
Cat. No. Size Price
defense as circulating monocytes and differentiation into
tissue macrophages and can differentiate into dendritic Human Peripheral Blood CD14+ Monocytes
cells with potent antigen-presenting capability, in vivo (cryopreserved)
and in vitro. 2W-400C ≥10 million cells

2W-400B ≥20 million cells
Monocytes are isolated from the peripheral blood of 2W-400A ≥40 million cells
screened, healthy donors. Peripheral blood is collected
from the donors using apheresis. The cell product, enriched
for mononuclear cells, is further processed by density
centrifugation to remove the remaining red cells and
neutrophils. Monocytes are then isolated using positive
immunomagnetic selection directed against CD14.
Immune System
Cells and Media
CD14+ Monocytes
■ Media Recommendations
– The cryopreserved cells can be stored in liquid nitrogen
until they are needed. Once thawed, the cells can be
cultured in a variety of different conditions. To maintain
the monocyte phenotype, serum-containing medium is
recommended (10% FBS is generally recommended).
M-CSF (at 10 ng/ml) can also be added. To produce
osteoclasts, both soluble RANK ligand (30 ng/ml) and
M-CSF (30 ng/ml) need to be added to serum-containing
medium. To produce dendritic cells, a serum-free medium
such as LGM-3® Lymphocyte Growth Medium-3 should
be used, with the addition of GM-CSF (50 ng/ml) and
IL-4 (50 ng/ml).
93
Human Dendritic Cells and Media
Poietics® Normal Human Dendritic Cells and Media Cells Ordering Information
consist of:
Cat. No. Size Price
— Primary derived cultures of NHDC - Normal Human
Dendritic Cells and/or HPBMC - Human Peripheral Blood NHDC – Normal Human Dendritic Cells
Recommended Medium: LGM-3®
Mononuclear Cells (cryopreserved)
— LGM-3® Lymphocyte Growth Medium-3, a serum-free CC-2701 2.5 × 106 cells
medium for lymphocyte cell growth
This system may be used for experimental applications HPBMC – Human Peripheral Blood Mononuclear Cells
(cryopreserved)
such as drug-induced immunosuppression of cytokine
secretion, antigen presentation and uptake studies, and CC-2702 ≥50 million cells
phenotypic studies of dendritic cells. CC-2702 ≥ 100 million cells
CC-2702 ≥ 200 million cells
All cells are isolated from peripheral blood by apheresis and
density gradient centrifugation. Dendritic cells, peripheral Medium Ordering Information
Immune System
blood mononuclear cells, and plasma are available from

Cells and Media
Cat. No. Size Price

the same donor.
LGM-3® Lymphocyte Growth Medium-3
Poietics® NHDC Cells do not proliferate and can survive up
CC-3211 500 ml
to 7 days in culture with the proper cytokines. Poietics®
HPBMC Cells, when properly stimulated, can divide in NOTE: Please inquire about availability of NHDC and HPBMC matched sets
from the same donor.
culture.
Dendritic cells
■ Media recommendations
– Poietics® LGM-3® Lymphocyte Growth Medium-3
contains albumin, insulin, transferrin, and gentamicin;
to complete NHDC differentiation, cells must be cultured
in IL-4 and GM-CSF
– All cells test negative for HIV-1, Hepatitis B and C,
mycoplasma, bacteria, yeast, and fungi
– After 4 days in culture, the NHDC stain positive for
CD11c and CD86 surface antigens, by flow cytometric
analysis
94
Human T and B Cells
Cord Blood CD4+ T Cells Cells Ordering Information

Poietics® Human Cord Blood CD4+ T Cells are isolated Cat. No. Size Price
from cord blood mononuclear cells using negative
Human Cord Blood CD4 T Cells
+
immunomagnetic selection. Purity is ≥85%. Human (cryopreserved)
cord blood CD4+ T cells are often used in studies of the
2C-200 ≥20 million cells
development of Th1/Th2 subsets.
Human Cord Blood CD19+ B Cells
Flow cytometric analysis depicting the purity of (cryopreserved)
Poietics® Human Blood CD4 + T Cells 2C-300A ≥5 million cells
2C-300A ≥2.5 million cells
2C-300B ≥1 million cells
Immune System
Cells and Media
Cord Blood CD19+ B Cells
Poietics® Cord Blood CD19+ B cells are isolated from cord
blood mononuclear cells using negative immunomagnetic
selection. Purity is ≥85%.
References
research
Peripheral Blood CD4+ T Cells Cells Ordering Information

Poietics® Human Peripheral Blood CD4+ T cells are a type Cat. No. Size Price
of lymphocyte that play an important role in the immune
Human Peripheral Blood CD4+ T Cells
system since they lead the attack against infections. (cryopreserved)
These cells are also referred to as “helper” cells. These
2W-200 ≥10 million cells
“helper” cells orchestrate the body’s response to certain
micro-organisms such as viruses. Research applications
include HIV and other autoimmune diseases, inflammatory
response, and immunotherapy.
Poietics® CD4+ T Cells are isolated from normal peripheral

blood mononuclear cells using negative immunomagnetic
selection of the CD4 antigen. Purity is ≥90%.
95
Human Natural Killer (NK) Cells
Human Natural Killer (NK) Cells are lymphocytes of

the immune system that are critical in host defense
and immune regulation. Since they are part of innate
immunity, they do not require sensitization for the
expression of their activity. NK cells play significant roles
in viral infections, autoimmunity, pregnancy, cancer,
bone marrow transplantation, and more recently, adaptive
immunity. NK cells are most traditionally characterized
by the presence of surface marker CD56, and absence of Human Natural Killer Cell
CD3. Poietics® Human NK Cells are isolated using positive
or negative immunomagnetic selection. Purity is ≥90%
via flow cytometry based on the presence of the CD56 Cells Ordering Information
antgen. Standard quantity is ≥5 million viable cells per
cryopreserved ampule after thawing. Cat. No. Description Size Price
Human Natural Killer Cells

Immune System
Cells and Media
(cryopreserved)
2W-501 Human NK Cells ≥5 million cells

(negative selection)
2W-502 Human NK Cells ≥5 million cells
(positive selection)
96
Human Neural Progenitor Cells and Media
Poietics® Neural Progenitor Cells and Media contains Cells Ordering Information
NHNP-Normal Human Neural Progenitor Cells and
Cat. No. Size Price
optimized medium for their sustenance. This cell system
can be useful for research applications in drug development, NHNP - Normal Human Neural Progenitor Cells
Recommended Medium: NPMM™ BulletKit®
neurotoxicity, neurogenesis, electrophysiology, CNS
PT-2599 ≥1.2 × 106 cells/ampoule
function, and neurotransmitter disorders.
NHNP - Cell Pellet in RNALater
Poietics® NHNP Cells are cryopreserved in primary passage
as “spheroids”. We guarantee that Poietics® NHNP will PX-2599RL ≈10 million cells/pellet
express markers described when plated onto laminin
coated plates.

– Poietics® Cells, Medium, and Reagents are quality
tested together and guaranteed for optimal performance Neural Progenitor Media
as a complete system Cat. No. Size Price
– All cells test negative for HIV-1, Hepatitis B and C, NPMM™ Neural Progenitor Maintenance Medium BulletKit®
mycoplasma, bacteria, yeast, and fungi Includes basal medium and SingleQuots® Kit
– Poietics® Normal Human Neural Progenitor Cells CC-3209 Kit

test positive for β-tubulin III (neurons) and GFAP
Neural Progenitor
Cells and Media
(astrocytes) NPDM™ Neural Progenitor Differentiation Medium BulletKit®
Includes basal medium and SingleQuots® Kit
We now offer two media kits specially formulated to CC-3229 Kit
support the growth, expansion, and differentiation of the
NHNP cells. NPBM™ Neural Progenitor Basal Medium
CC-3210 200 ml
NPMM Neural Progenitor Maintenance Medium BulletKit®
contains the necessary supplements and media for optimal NPMM™ Neural Progenitor Maintenance Medium
NHNP cell growth and differentiation. This kit includes: SingleQuots® Kit
– CC-3210 – Neural Progenitor Basal Medium CC-4241 —
– CC-4241 – Neural Progenitor Maintenance Medium Neural Progenitor Supplement SingleQuots® Kit
SingleQuots® Kit (contains hEGF and hFGF)
CC-4242 —
– CC-4242 – Neural Progenitor Supplement SingleQuots®
Kit (contains NSF-1 and GA) For related neural cell and media systems, see page 49, 73 – 76
NPDM™ Neural Progenitor Differentiation Medium

BulletKit®
contains the necessary supplements and media for optimal
NHNP differentiation. The medium can be customized by
supplementation with differentiation-promoting agents such
as Brain Derived Neurotrophic Factor. This kit includes:
– CC-3210 – Neural Progenitor Basal Medium
– CC-4242 – Neural Progenitor Supplement SingleQuots®
Kit NHNP stained for β-tubulin III and GFAP
References
1. Sold under license from StemCells, Inc. US patents 5,968,829 and
— For a comprehensive list of references and additional 5,851, 832.
research
97
Custom Cell Isolation Services
Consult the cell culture experts and harness the power of n D

elaysin obtaining vital primary cell cultures can be
Lonza’s extensive knowledge and experience delivering very costly. Discover our superior custom cell isolation
custom cell solutions. Choose from an endless variety of capabilities including:
human and animal primary cell types, cryopreserved, – Primary cell isolations from human and animal
plated or in culture flasks, for your convenience. Save tissues
time and money by avoiding the aggravation of tissue
acquisition, failed isolations and low yields. – Cell expansion and testing
– Donor-matched cell sets
■ Quality and experience – Cryopreserved or proliferating formats
As the cell culture experts, Lonza maintains an – Wide array of QC and cell characterization services,
ISO 9001:2001 certified custom cell isolation laboratory including PCR
staffed by some of the world’s finest cell culture – Individual customer consultation to ensure correct
technicians, providing a variety of cell types for your order fulfillment
individual research needs. Partner with Lonza and benefit
from over 60 years of combined cell culture isolation
n C
ustom primary cell isolations from human, rat, mouse,
experience.
porcine, bovine, monkey and many other species.
Examples of successful isolations include:
■ We offer
– Bovine adrenal gland capillary endothelial cells
Custom Cell Isolation
– Expert custom cell isolation solutions made to your

– Bovine embryonic kidney cells
specifications
Services
– Bovine and porcine preadipocytes

– Extensive quality testing and cellular charac-
terization available – Canine umbilical vein endothelial cells
– Years of isolation experience with a wide variety of – Cat iris smooth muscle cells
cell types – Guinea pig kidney cells
– Custom formats: cryopreserved, plates or flasks – Human bladder epithelial cells
– State-of-the-art cell isolation capabilities – Human bronchial/tracheal epithelial cells – Cystic
Fibrosis
– Human diabetic CD34+ cells
– Human microvascular retinal endothelial cells
– Human small intestine disassociated cells
– Murine dermal fibroblasts
– Porcine mesangial cells
– Rat mesenchymal stem cells
98
Clonetics® FAQs
Media & Reagents

Q. What is a BulletKit®? Q. What is a complete cell and media system?
A. A BulletKit® contains a bottle of basal medium A. A complete cell and media system allows the
and a SingleQuots® Kit. It is possible to purchase growth, subculture and continued growth of
each of these components separately. Once normal human cells in vitro (in cell culture
the basal medium is supplemented with the plastic). A Clonetics® Cell and Media System
SingleQuots® Kit, it becomes a complete growth includes three components: one cryopreserved
medium. ampoule or proliferating cell culture,
Q. What are SingleQuots® Supplements and Growth Clonetics® Optimized Growth Media, and a
Factors? ReagentPack™ Subculture Reagents containing
all reagents necessary for subculturing the
A. SingleQuots® Supplements and Growth Factors cells. All of our products are quality tested
contain several vials that may include growth using the complete Clonetics® Cells and Media
factors, FBS, and antibiotics in pre-measured System and must conform to standardized
aliquots designed to be added to a bottle of basal cell culture performance before release. Cells,
medium. The concentrations of these growth media, and reagents must all be purchased
factors are proprietary. separately.
Q. Do I need to add antibiotics to your media? Q. Do I need to use a gelatin, fibronectin, collagen,
or Matrigel® coating?
A. For most kits, gentamicin-amphotericin-B mixture A. Most Clonetics® Cells do not require an
is provided at a final concentration of 30 µg/ml extracellular matrix when grown on tissue
gentamicin and 15 ng /ml amphotericin-B. culture treated plastic – flasks, dishes, well
Clonetics® FAQs
Antibiotics are not provided for the KGM®-CD Kit plates (with the exception of hepatocytes,
and will need to be added if you so desire. bovine brain microvascular endothelial cells, and
the rat/mouse neuronal and dorsal root ganglion
Q. Can I use a medium other than the one you offer
neurons, rat cardiac myocytes which do require a
to culture the Clonetics® Cells?
coating). However, we have found that glass
A. We have an optimized medium for each Clonetics® slides, chamber slides, and other formats may
Cell type. The cells are grown and tested in their require a coating prior to seeding the cells.
specific medium system. We cannot guarantee Please contact Scientific Support for further
cell performance if our recommended medium is information.
not used. Q. Can Clonetics® Cells be transfected?
Q. What is the difference between the EGM® A. Yes, see pages 191, 203-205 for transfection
BulletKit® (CC-3124) and the EGM®-2 BulletKit® reagents that are optimized to work with our
(CC-3162)? primary cells.
A. The primary difference between the two media Q. Your instructions do not mention centrifuging
types is in the growth factors. The EGM® the amp of cells upon thawing. Won’t the DMSO
BulletKit® contains: BBE, hEGF, hydrocortisone, kill the cells?
FBS, and GA-1000. In the EGM®-2 BulletKit®, A. Clonetics® Normal Human Cells are very
we have removed the BBE and replaced it with fragile out of cryopreservation and should
known growth factors: VEGF, hFGF-B, ascorbic not be centrifuged (exception – bovine brain
acid, insulin-like growth factor, and heparin. microvascular endothelial cells and hepatocytes).
The cells proliferate much better in the EGM®-2 Residual DMSO will be removed by a media
Medium. It has been reported that the EGM® change after 24 hours.
BulletKit® works better for VEGF, FGF, and
angiogenesis research. Q. I thawed and plated my cells yesterday and
most of the cells are floating, what happened?
A. Clonetics® Cells will not be confluent on day
one. After 24 hours, you will see few attached
cells per field under the microscope. Aspirate
off the media and the floating cells and replace
with fresh, pre-warmed media. Those attached
cells will begin to proliferate and a confluent
monolayer should be established in 5 –10 days.
99
Clonetics® FAQs
Continued
Media & Reagents

Q. I’ve had my cells in culture for a few months, Q. Can I seed my ampoule of cells into one T-25
they’re no longer growing and don’t look like flask?
healthy cells, what’s wrong?
A. No. Each cell type has a recommended seeding
A. All Clonetics® Cells are primary cells with a finite density optimized for cell attachment and
life span. Most Clonetics® Cells are guaranteed proliferation. Using information from the
for 10 – 15 population doublings, which equates Certificate of Analysis shipped with each
to about 2 – 3 passages. As cell death begins cryopreserved vial of cells, use the following
to occur, morphology and cell performance equations to calculate the number of flasks or
will begin to change and growth will slow dishes that can be set up:
drastically.
Number of cells per amp ×
percent viability Maximum number of cm2
Q. I don’t see the specific cell type I want in your =
Recommended seeding that can be inoculated
catalog or on your website. Do you do custom density
isolations?
A. Yes, Lonza offers custom isolations and formats Maximum number of cm2 that Maximum number of
can be plated = culture vessels that can
for various types of non-catalog cell products.
Effective growth area of flask be set up
Please see pages 79 & 98 for additional
information.
*Note: Cells seeded into well plates should be seeded at
10,000 viable cells/cm2.
Clonetics® FAQs
100
Clonetics® Technical Information: Cells and Media
Overview of cell culture process for Clonetics® Human and Animal Cells*
Unpack and store cells
▼
BulletKit® Supplemented Media
Prepare Medium
▼ ▼
Add SingleQuots® Kit Add BPE or BBE
Proliferating Cultures Prepare Cells Cryopreserved Cultures
▼ ▼
Storage Requirements: Calculate number of flasks/
Incubate 3 – 4 hours
chambers/dishes required
Cells
Upon arrival, immediately remove cryo-
preserved cells from dry ice and place Add medium to culture vessel(s)
immediately into liquid nitrogen. If no Replace medium and incubate
and incubate for at least 30
Technical Information
dry ice is left in the package, thaw cells, minutes
immediately place them into culture
vessels and call your Technical Specialist.
Clonetics®
Medium Thaw cells in water bath
Store Clonetics® Cell Culture Medium in a

4°C refrigerator. When using medium, under
sterile conditions, take the amount you Resuspend cells in cryovial
need and return bottle to the refrigerator.
Always bring medium to room temperature
before use.
Dispense equal amounts of cells
Supplements and Reagents into flask
If you to plan to subculture within three
days, store all growth supplements, HEPES
Buffered Saline Solution and Trypsin
Neutralizing Solution at 4°C. Trypsin/EDTA 1–2 ▼ 5–9
Solution has a limited shelf life at 4°C.
If, upon arrival, Trypsin/EDTA is thawed,
DAYS Maintenance: Every 40 hours DAYS
change medium, increase as
immediately aliquot and refreeze at necessary
-20°C. If Trypsin/EDTA is frozen, store at
-20°C. If you do not plan to set up the cell
culture within 3 days, store all growth
supplements and subculture reagents in a When at 60 – 90% confluence,
-20°C freezer. subculture
Assess cell yield and viability,

optimize as necessary
Maintenance: After 24 hours,

change medium
Incubate and re-examine culture
*Exceptions: NHEM, Rat and Mouse neural cells, Rat Cardiac Myocytes,
h NHEPS®, sm NHEPS®, rt NHEPS®, and bMVEC-B
101
Safety Precautions with Clonetics® Cells
— As a precaution against contamination, follow all procedures for handling products of human origin outlined in
“Guidelines to Avoid Personnel Contamination By Infective Agents in Research Laboratories That Use Human Tissues,”
from the J. of Tissue Culture Methods.
— Always wear gloves and safety glasses when working with all materials. Exercise caution when working with cryopreserved
cells; rapid temperature changes may cause splattering of liquid nitrogen.
— Wash hands thoroughly after performing all procedures.
— Do not smoke, eat or drink in areas where reagents or cells are handled.
— Never pipette by mouth.
— Products of human and animal origin are potential biohazards. Although provided human cells negative for HIV-1,
Hepatitis B and C, proper precautions must be taken to avoid inadvertent exposure.
WARNING: Clonetics® and Poietics® Products contain human sourced material. Treat as potentially infectious.
Media Preparation
Clonetics®
Perform the following steps in a sterile field For BulletKit®, perform the following steps
For a bottle of fully supplemented medium, do the 1. Decontaminate the external surfaces of the
following: SingleQuots® Cryovials and the basal media bottle
1. Add BBE or BPE, if required, to a 500 ml bottle of with ethanol or isopropanol.
basal medium.
2. Aseptically open each cryovial and add the entire
2. Detach the BBE or BPE supplement from the medium amount to the basal medium with a pipette.
bottle.
3. Rinse each cryovial with the medium. It may not
3. Decontaminate the vial and medium bottle with be possible to recover the entire volume listed for
ethanol or isopropanol. each cryovial; small losses, even up to 10%,
4. Add the entire contents of the vial (approximately should not affect the cell growth characteristics of
2 ml) to the medium with a pipette; rinse the vial the supplemented medium.
with medium and pipette the contents back into the 4. Transfer the label provided with each kit to the basal
500 ml bottle. medium bottle being supplemented. Use it to record
5. Replace the cap and swirl the medium gently a few the date and amount of each supplement added; we
times to mix. recommend that you place the completed label over
the basal medium label to avoid confusion or possible
6. Record the date the BBE or BPE was added on the double supplementation.
medium label.
5. Record the new expiration date on the label based on
the shelf life. A fully reconstituted BulletKit® should
be used within 30 days; this supplemented medium
will now be referred to as a growth medium.
NOTE: If there is concern that sterility was compromised during the
supplementation process, the entire newly prepared growth medium may
be re-filtered to assure sterility. If you re-filter, use a sterile 0.2-micron filter.
Routine re-filtration is not recommended as some protein loss may occur
with each filtration.
Continued on next page
102
Media Preparation
Continued
Before You Begin

Perform the following steps before you begin media or cell preparation:
1. Prepare a sterile field: 4. Other required supplies:
– A sterile field consists of a Class II biological safety – 70% alcohol (ethanol or isopropanol)
cabinet with a front access opening and filtered – Growth medium (cell type specific)
laminar airflow, or an equivalent device
– Protective gloves and garments
2. Determine the amount of medium required:
– Review the Growth Area of Common Plasticware table – Trypan Blue
to determine the amount of medium to be used 5. Plan and prepare for initial set up:
3. Sterile instruments and vessels required: – Base the set-up on the number of cells indicated
– Sterile disposable serological pipettes on the Certificate of Analysis accompanying the
product
– Micropipettes and sterile pipette tips
6. Check the calibration on humidified incubator.
– Adjustable multichannel pipette or repeating pipette Incubator should be humidified and set to 5% CO2,
– Sterile reservoirs for use with multichannel pipette 95% air, and 37°C.
– Sterile 15 ml centrifuge tubes
– Cell culture flasks, or multiwell, flat-bottom tissue
culture plates
– Hemacytometer or cell counter
Clonetics®
Growth Area of Common Plasticware
Initial Number Initial Number Initial Number

Effective Cell Culture of Cells to Seed of Cells to Seed at of Cells to Seed at
Flasks Growth Area Medium Required at 2,500 cells/cm2 3,500 cells/cm2 5,000 cells/cm2
T-25 25 cm2 5 ml 62,500 87,500 125,000
T-75 75 cm2 15 ml 187,500 262,500 375,000
T-150 150 cm2 30 ml 375,000 525,000 750,000
Initial Number Initial Number Initial Number

Effective Cell Culture of Cells to Seed of Cells to Seed at of Cells to Seed at
Dishes Growth Area Medium Required at 2,500 cells/cm2 3,500 cells/cm2 5,000 cells/cm2
35 mm 9.6 cm2 2 ml 20,000 28,000 40,000
60 mm 21 cm2 5 ml 52,500 73,500 105,000
100 mm 55 cm2 11 ml 137,500 192,500 275,000
150 mm 148 cm2 30 ml 370,000 518,000 740,000
Effective Cell Culture Initial Number

Multiwell Growth Area Medium Required of Cells to Seed
Plates Per Well Per Well/Total at 10,000 cells/cm2
6 well 9.60 cm2 2 ml / 12 ml 96,000
12 well 3.80 cm2 1 ml / 12 ml 38,000
24 well 2.00 cm2 0.5 ml / 12 ml 20,000
48 well 0.75 cm2 150 µl / 7 ml 7,500
96 well 0.32 cm2 100 µl / 10 ml 3,200
103
Culture Set Up – Adherent Cell Types
These instructions do not apply to bMVEC-B, hepatocytes, The advantage of setting up this number of T-25 flasks
NHEM, rat & mouse cells, and Poietics® Cells. from the initial cryovial, as opposed to larger flasks, is
1. Calculate the number of vessels to be set up. that it reduces the risk of losing large numbers of cells.
Refer to your Certificate of Analysis for the exact That is, if you experience difficulty trypsinizing the first
number of cells in your cryovial. Refer to table on T-25 flask, there are other remaining T-25 flasks to use.
page 103 “Growth Area of Common Plasticware”, 2. Label each flask with the passage number, cell
for help in adjusting this calculation. type, lot number, and date.
Use the following calculations to determine the number 3. In a sterile field, carefully open the supplemented
of vessels to be set-up for the recommended seeding bottle of growth medium and aseptically transfer
densities of 2,500 cells/cm2, 3,500 cells/cm2, or 5,000
the medium to new culture vessels by adding 1
cells/cm2
ml growth medium for every 5 cm2 surface area
No. of cells available × Percent viability Max. no. of cm2 that can of the flask.
=
Recommended seeding density be plated
Example: 5 ml growth medium for a 25 cm2 flask.
Max. no. of cm2 that can be plated Max. no. of flasks that 4. Tighten vented caps on vessels. If vented caps
=
Effective growth area of flask can be set up
are not being used, twist caps until tight, then
Example: A cryovial of HMVEC-L with 520,000 cells and 80% viability
loosen about 1/2 turn. Allow the culture vessels
to warm and equilibrate in a 37°C, 5% CO2,
520,000 × 0.80
= 83 cm2, to be set up humidified incubator for at least 30 minutes.
5,000
If you use a T-25 with an effective growth area of 25 cm2

Clonetics®
83 cm2 3 flasks (rounded down to the nearest

=
25 cm2 whole number of flasks)
Thawing Cells – Adherent Cell Types
Aseptically add the recommended amount of medium to the color of the thawed cryovial should be pink.
the flask and equilibrate for 30 minutes in a 5% CO2, 37°C If the color is not pink, seed the cells, note the
incubator. color and mention this fact to Scientific Support
1. Have a micropipette ready prior to thawing. if seeding is not successful.
2. Remove the cryovial of cells from storage. Wipe NOTES:
cryovial with ethanol or isopropanol before – If more than one cryovial is to be thawed, thaw one cryovial
at a time and keep other cryovials in liquid nitrogen until
opening. In a sterile field, briefly twist the cap a ready for use
quarter turn to relieve the internal pressure, then – Cryopreserved cells are very delicate; thaw and return them
retighten; do not open the cryovial completely. to culture as quickly as possible with minimal handling
3. Holding the cryovial, dip the bottom 3/4 of the – Wear eye protection when handling frozen cells; rapid
temperature changes may cause splattering of liquid
cryovial in a 37°C water bath and swirl gently for nitrogen
1 – 2 minutes until contents are thawed. Watch – Centrifugation should not be performed to remove cells
the cryovial closely; when the last sliver of ice from the cryoprotectant cocktail; this action is more
damaging than the effects of DMSO residue in the culture
melts remove it; DO NOT submerge it completely.
– It is not recommended to thaw frozen cells directly onto
Thawing the cells for longer than 2 minutes may glass slides, chamber slides, gridded plates or multiwell
result in less than optimal results. plate configurations (6, 12, 24, 96…); optimal performance
is achieved when initial seeding out of cryopreservation is
4. Remove the cryovial immediately, wipe it performed into T-25 flasks; for further instructions follow
dry, and transfer to a sterile field where the the directions in the set-up section in the cell culture
instructions provided
equilibrated flasks should be waiting, ready to
seed. Rinse the cryovial with 70% alcohol, then
wipe to remove excess.
5. Note the color of the thawed cryovial. Ideally,
104
Seeding – Adherent Cell Types
Remove the cap, being careful not to touch the interior After Seeding
threads with your fingers. Cells are not tolerant to rapid temperature fluctuations
1. Using a 1,000 µl micropipette set to 800 µl, put the or nutrient-deficient medium. Feeding them with fresh
tip into the cryovial and resuspend the cells with growth medium that has been warmed will avert potential
a gentle, slow and steady up and down pipetting problems. (Remember to warm only the amount needed.)
motion no more than five times. DO NOT resuspend Check and feed the cells on the schedule below, even on
quickly and keep the tip near the bottom to avoid weekends and holidays.
making bubbles. 1. Change the growth medium the day after seeding
2. Dispense an equal amount of cells into the flasks (to remove residual DMSO and unattached cells),
set up earlier (as determined by the recommended then every other day thereafter while examining
seeding density and number of cells/vial). If four them daily.
T-25 flasks were prepared, set micropipette to 250 NOTE: A change of medium requires removal of the medium by aspirating
with a sterile pipette on the opposite side of the flask from where the cells
µl and dispense. If eight T-25 flasks were prepared, are attached. Then warm, fresh medium is added down the same side.
set micropipette to 125 µl and dispense.
3. Replace the cap or cover and gently rock the 2. Successfully recovered cultures will exhibit the
vessels to evenly distribute the cells. Loosen caps if following:
a. Cells with clear non-granular cytoplasm.
necessary to permit gas exchange. b. Numerous mitotic figures after day 2.
4. Return the culture vessels to a 37°C incubator 3. Feed the cells a larger volume of medium as
with 5% CO2. Lay them flat on the shelf, providing
they become more confluent. Use this table as a
the largest surface for cells to attach. The cells will guideline:
anchor to the bottom surface of the flask. If cells are: Then feed them:
Clonetics®
Under 25% confluent… 1 ml per 5 cm2
NOTE: Do not dispense the entire contents of the cryovial into one T-25 From 25 – 45% confluent… 1.5 ml per 5 cm2
flask! Exceeding 45% confluence… 2 ml per 5 cm2
4. Continue feeding the cells until 60 – 90%

confluence. If specific cell types are allowed to
become over-confluent (i.e., epithelial cells) and
stay at confluence for more than 2 days, they
can suffer irreversible contact inhibition and
may detach from the flask and/or be difficult to
trypsinize.
Proliferating Cells – Adherent Cell Types
1. Examine the culture microscopically for any signs to 37°C in a sterile container. Warming the entire
of distress during shipment (i.e., detachment, bottle can shorten the life of the medium. Never
rounding-up, or atypical morphology). Check the warm medium under hot running water or any
relative cell density and estimate % confluency. The other uncontrolled temperature source. NEVER
culture should be 30 – 100% confluent upon receipt. MICROWAVE.
Some cellular detachment is normal. Please call 5. In a sterile field, carefully open the cell culture flask
Scientific Support immediately if cells look severely or multiwell dish, remove the medium and replace it
distressed. with the warmed, fresh medium. Aseptically remove
2. Decontaminate the external surface of the cell culture any medium inside the neck or cap area because it
flask or multiwell dish by wiping with 70% ethanol or can facilitate microbial contamination.
isopropanol. 6. If you are using a flask with a non-vented cap, loosen
3. Incubate the sealed flask or multiwell dish at 37°C, 5% the cap and return the flask to the 37°C humidified
CO2, for 3 – 4 hours to equilibrate temperature. incubator with 5% CO2 for at least 24 hours.
4. Warm an appropriate amount of growth medium
105
Subculturing – Adherent Cell Types
Storage Information for Subculture Reagents In a sterile field:

1. Subculture reagents are sterile-filtered and then (T-25 flask used as example. Increase volume proportionately for others flasks.)
stored at -20°C until shipped from Lonza’s Distribution 1. Aspirate the medium from one culture vessel.
Centers. 2. Rinse the cells with 5 ml of room temperature
2. Subculture reagents may thaw during transport. HEPES Buffered Saline Solution (HEPES-BSS). DO
They may be refrozen once. NOT forget this step. The medium contains complex
proteins that neutralize the trypsin.
3. Subculture reagents can be stored at -20°C until
expiration date, after thawing once and refreezing. 3. Aspirate the HEPES-BSS from the flask.
4. To keep Trypsin/EDTA fresh and active after thawing, 4. Cover the cells with 2 ml of Trypsin/EDTA solution.
you may aliquot it into sterile centrifuge tubes and 5. Tighten the cap and begin monitoring the flask
refreeze at -20°C. Trypsin/EDTA may be stored frozen under the microscope.
until the expiration date.
6. Continue to examine the cell layer microscopically.
5. We recommend that HEPES-BSS and the Trypsin a. Allow the trypsinization to continue until approximately 90% of
Neutralization Solution once stored at 4°C be used the cells are rounded up.
within one month. NOTE: Rounded up cells are spherical, have smooth edges and are refractile
or shiny. If the cells still have protruding nubs which are still attached to the
The following instructions are for a 25 cm2 flask. flask, they need more time to trypsinize. This entire process takes about
2 – 6 minutes, depending on cell type.
Adjust all volumes accordingly for other size flasks.
b. At this point, rap the flask against the palm of your hand to
Preparation release the majority of cells from the culture surface. If only a few
cells detach, you may not have let them trypsinize long enough.
Preparation for subculturing the first flask:
Clonetics®
Wait 30 seconds and rap again. If cells still do not detach, wait
1. Subculture the cells when they are 60 – 90% confluent and rap every 30 seconds thereafter.
and contain many mitotic figures throughout the NOTE: Do not try to get all cells to detach by rapping them severely. This
action may damage the cells.
flask.
2. For each 25 cm2 of cells to be subcultured: 7. After cells are released, neutralize the trypsin in
a. Thaw 2 ml of Trypsin/EDTA and allow to come to room temperature.
the flask with 4 ml of room temperature Trypsin
b. Allow 7 - 10 ml of HEPES Buffered Saline Solution (HEPES-BSS) to Neutralizing Solution. If the majority of cells do
come to room temperature. not detach within seven minutes, the trypsin is
c. Allow 4 ml of Trypsin Neutralizing Solution (TNS) to come to room either not warm enough or not active enough to
temperature.
release the cells. Harvest the culture vessel as
3. Remove growth medium from 4°C storage and allow described above, and either re-trypsinize with
to start warming to room temperature. fresh, warm Trypsin/EDTA Solution or rinse with
4. Prepare new culture vessels: Trypsin Neutralizing Solution and then add fresh,
a. Prepare 1 – 3 T-75 flasks. The number of flasks needed depends warm medium to the culture vessel and return to
upon confluence and total yield. Larger flasks may be used to an incubator until fresh trypsinization reagents are
save plasticware and time spent on subsequent subcultures.
Smaller flasks reduce the risk of losing a substantial part of your available.
culture.
b. As before, label each flask with the passage number, lot number, 8. Quickly transfer the detached cells to a sterile
cell type, and date. 15 ml centrifuge tube.
c. In a sterile field, carefully open the bottle and transfer growth
medium to new culture vessels by adding 1 ml growth medium 9. Rinse the flask with a final 2 ml of HEPES-BSS to
for every 5 cm2 surface area of the flask. collect residual cells, and add this rinse to the
Example: 15 ml growth medium for a 75 cm2 flask centrifuge tube.
d. If not using vented caps, loosen caps of flasks. Place the new
culture vessels into a 37°C humidified incubator with 5% CO2 10. Examine the harvested flask under the microscope
and equilibrate the flasks for at least 30 minutes. Subculture
one flask at a time. All flasks following the first flask will be
to make sure the harvest was successful by looking
subcultured following an optimization of this protocol (explained at the number of cells left behind. This should be
later in this procedure), based on calculated cell count, cell
viability, and seeding density.
less than 5%.
11. Centrifuge the harvested cells at 220 × g for 5 minutes
NOTE: Use only Clonetics® Trypsin/EDTA. The concentration of Trypsin/EDTA
from other suppliers may be 10X our concentration, which will detrimentally to pellet the cells.
effect Clonetics® Cells. a. Aspirate most of the supernatent, except for 100 µl to 200 µl.
b. Flick the centrifuge tube with your finger to loosen the pellet.
106
Subculturing – Adherent Cell Types
Continued
12. Dilute the cells in 4 ml to 5 ml of growth medium 21. After mixing the diluted cells with a 5 ml pipette to
and note the total volume of the diluted cell ensure a uniform suspension, dispense the volume
suspension. of suspension calculated above into the prepared
NOTE: To obtain the best results from your cells, assess cell yield and subculture flasks.
viability with Trypan Blue.
22. After dispensing the cells, gently rock flask to
13. Count the cells with a hemacytometer or cell promote even distribution.
counter and calculate the total number of cells.
(See instructions above.) Make a note of your cell 23. If not using vented caps, loosen caps of flasks.
yield for later use. Place the new culture vessels into a 37°C humidified
NOTE: The cell suspension should contain between 250,000 to
incubator with 5% CO2.
1,000,000 cell/ml for greatest accuracy.
14. If necessary, dilute the suspension with HEPES

Buffered Saline Solution (HEPES-BSS) to achieve
the desired “cells/ml” and re-count the cells.
15. Assess cell viability using Trypan Blue.
16. Use the following equation to determine the total
number of viable cells.
Total cell count × percent viability = Total # of viable cells
Example: 1,000,000 cells × 60% = 600,000 viable cells
17. Determine the total number of flasks to inoculate
Clonetics®
by using the equation below. The number of flasks
needed depends upon cell yield and seeding density.
Larger flasks may be used to save plasticware and
time spent on subsequent subcultures. Smaller
flasks reduce the risk of losing a substantial part of
your culture if contamination occurs.
The recommended seeding density could be 2,500 cells/cm2, 3,500 cells/cm2,
or 5,000 cells/cm2 for flasks and 10,000 cells /cm2 for well plates
Total # of viable cells Total # of flasks to

=
Recommended seeding inoculate
density × flask size
600,000 viable cells 3 T-75 flasks (rounded down to

=
75 cm2 × 2,500 cells/cm2 nearest whole number)
18. Use the following equation to calculate the volume

of cell suspension to seed into your flasks.
Total volume of diluted cell suspension
= Seeding volume
# of flasks as determined in step 17
4.3 ml of diluted cell suspension

= 1.43 ml per T-75 flask
3 T-75 flasks
19. Prepare flasks by labeling each flask with the

passage number, lot number, cell type, and date.
20. Carefully open the medium bottle and transfer
growth medium to new culture vessels by adding
1 ml growth medium for every 5 cm2 surface area
of the flask (1 ml/5 cm2).
Example: 15 ml growth medium for a 75 cm2 flask.
107
Subculturing into 96-well plates
Overview Procedure
A culture flask of normal human cells is harvested by 1. Follow the steps for subculture preparation and
trypsinization and subsequent trypsin inhibitor treatment. subculturing. Then follow steps 2 – 4 below.
The cells are centrifuged, resuspended in growth medium 2. Since the cells/ml calculation computed is per ml,
and counted. The desired number of cells is then added the cell concentration must be increased by 4 times
to wells of sterile 96-well tissue culture plates. The plates before seeding 96-well plates (to accommodate the
are incubated in a 37°C, 5% CO2 humidified incubator for 1 1:4 dilution when adding 250 µl of suspended cells
– 3 days to allow for cell adherence and growth. Seeding per well). When making the cell suspension, adjust
densities will vary somewhat with your experimental the cell concentration with growth medium.
requirements. A density of 10,000 cells/cm2 for multiwell 3. Transfer the diluted cell suspension to a sterile
plates is ideal. See the Cell Culture Instruction Sheet* for the reservoir. Using a multichannel (8 or 12 channel)
cell type you are using for specific information. pipette equipped with sterile pipette tips, add 250
*Available at www.lonza.com/research or contact Scientific Support. µl of the diluted cell suspension to each well of the
labeled 96-well flat bottom, tissue culture plate(s).
Required Materials: RESUSPEND THE CELL SUSPENSION OFTEN DURING THE SEEDING
1. T-25 flask of proliferating normal human cells between PROCEDURE TO ENSURE A UNIFORM NUMBER AND DISTRIBUTION OF
60% and 90% confluence CELLS INTO EACH WELL BY PIPETTING UP AND DOWN A FEW TIMES
BETWEEN EVERY OTHER DISPENSING.
2. 96-well flat bottom, tissue culture plates
3. 37°C humidified incubator with 5% CO2/95% air 4. Cover and incubate the plates for 1 – 3 days at
4. Laminar flow hood or other sterile environment 37°C/5% CO2. (Incubation periods exceeding 3 days
5. Adjustable multichannel pipette (8 or 12 channel) or are generally not recommended because of evaporation
Clonetics®
repeating pipette of medium from the edge wells of the plate).

6. Sterile reservoir(s) for use with multichannel
Note: Before using the 96-well plate culture in a bioassay, examine the cells
pipette microscopically for the presence of mitotic figures as a confirmation that
the cells have resumed active growth.
108
Instructions for Cryopreservation
Cryopreservation may compromise cell quality and Cryopreservation Solutions

performance. Performance of the cells cannot be
For mesenchymal stem cells
guaranteed after cryopreservation. These instructions do
70% MSCBM
not apply to hepatocytes, bMVEC-B, melanocytes, mouse
10% DMSO
and rat neuronal cells, rat cardiac cells, and Poietics®
20% Human Serum Albumin (25% solution)
Cells.
For skeletal muscle cells
Instructions
70% SKGM®
1. Sterile filter freezing medium using a cell culture 20% FBS
rated 0.2-micron filter. 10% DMSO
2. Harvest cells and spin them down.
For all other cell types
3. Resuspend cells in cold freezing solution at 80% Clonetics® Growth Medium of choice
≈500,000 to 2,000,000 cells/ml. Work quickly. 10% FBS
Once exposed to DMSO, cells become very fragile. 10% DMSO
4. Pipet aliquots (1 ml each) into freezing vials or
ampoules and seal.
5. Insulate aliquots with a Styrofoam® or propanol
freezing canister.
6. Store cells at -70°C overnight.
Clonetics®
7. Within 12 – 24 hours, place in LN2 (-200°C) for
long-term storage. Cells will be compromised by
prolonged storage at -70°C.
109
Improving Cell Yield and Viability During Subculture
Several factors, or a combination of factors, can contribute to low cell count and low cell viability. If cell yield or viability is
unsatisfactory, use the following information to increase the success rate of future cultures.
Improving Cell Yield

If your cell yield is low (less than 50%), determine the cause(s) and possible solution(s) using the table below. Then subculture
one more flask applying the appropriate solution(s).
Low Yield (Cell Count)
Condition Possible Causes Solutions

Majority of cells did not detach 1. Inactive or cold Trypsin/EDTA 1. Use Trypsin/EDTA at room temperature
2. Improper storage of Trypsin/EDTA 2. Store at -20°C until ready for use; thaw and allow
it to come to room temperature briefly before
subculturing
3. Exposure time to Trypsin/EDTA was too short 3. Exposure time to Trypsin/EDTA is usually 5 –6
minutes
4. Trypsin/EDTA has been neutralized 4 .Be sure to rinse the culture completely with
HEPES-BSS before trypsinization
5. Vessel was not rapped firmly 5. Use a moderate amount of force when rapping
during trypsinization
Low yield, 95% of the cells Culture was under confluent at trypsinization Be sure to trypsinize at 60 – 90% confluence with
detached but the yield was low numerous mitotic figures throughout the flask
Clonetics®
If your cell viability is low (less than 50%), determine the possible cause(s) and solution(s) using the table below. Then subculture
one more flask applying the appropriate solution(s).
Low Viability (<50% viable)
Condition Possible Causes Solutions

Trypsin/EDTA damaged the cells 1. Used another vendor’s Trypsin/EDTA 1. Use only Clonetics® Trypsin
2. Exposure time of the cells to Trypsin/EDTA was too long 2. Do not trypsinize longer than 7 minutes
3. Trypsin/EDTA was used above room temperature. 3. Do not use even mildly heated Trypsin/EDTA
Trypsin becomes more active at temperatures above
room temperature
4. Failed to neutralize the trypsin. 4. Neutralize the Trypsin/EDTA with Trypsin
Prolonged exposure to trypsin will damage cells. Neutralizing Solution to eliminate cell damage
due to trypsin
5. Vessel was rapped too firmly during trypsinization. 5. Use moderate force when rapping flask to
Rapping too hard to release cells causes cell membrane dislodge cells during trypsinization
damage
Culture vessel was too confluent; Culture was too confluent at trypsinization Be sure to trypsinize at 60 – 90% confluence with
was completely covered with cells about five mitotic figures per field of view
Cell growth slowed before The most probable cause is failure to increase the Change medium and increase volume as
80% confluence and cells look volume of medium used as the cell confluency increased recommended. Please observe all guidelines
dull and non refractile The cells become mildly starved and are not able to
recover after trypsinization
110
Clonetics® Media
Medium Specifications Complete Medium

Medium is formulated for optimal growth of specific types Complete medium is fully supplemented growth medium
of normal human cells. It can be purchased as basal (BPE and BBE are packaged separately) and contains all
medium without supplements, as fully supplemented of the growth factors and supplements necessary for the
growth medium, or in a conveniently packaged BulletKit® propagation of specific types of normal human cells in
that allows user control over supplement type and culture. Undefined supplements are avoided when possible
concentration. Each type of medium is tested for its and used only at minimal levels when necessary. Standard
ability to support growth of the intended normal human formulations of all growth media include antimicrobials.
cell. Biochemical and sterility tests are also performed on Antimicrobial-free media are available as a special order.
every lot of medium. Certificates of Analysis are available
upon request for all medium products. BulletKit®
A BulletKit® provides flexibility in final medium formulation
Basal Medium
and increased shelf life. Each BulletKit® contains
Basal medium has been optimized for specific types of basal medium and premeasured, single-use aliquots
normal human cells. Basal medium does not contain (SingleQuots®) of growth factors and antimicrobial agents
growth factors necessary for propagation of cells. Growth to formulate the fully supplemented growth medium of
factors must be added to enhance plating efficiency and your choice.
cellular proliferation. Optimized formulations make it
possible to perform research on a wide variety of normal
Clonetics® Media
human cell types. Results of years of media development
are available to you for use in your own research.
111
Airway Cell Media Options
Airway cell media are serum-free media that have BEGM® BulletKit®
been optimized for the proliferation of airway cells.
— Best growth of NHBE - Normal Human Bronchial/
Each component of the basal medium and each growth
Tracheal Epithelial Cells in medium containing
supplement are carefully titered for optimal growth by
antimicrobials
Lonza’s R&D team. Lonza currently offers two media
choices for the growth of airway cells, allowing for desired
performance and formulation flexibility. When selecting a SAGM™ BulletKit®
medium to use, refer to specific media recommendations
— Superior growth for SAEC - Human Small Airway
or call Scientific Support for assistance.
Epithelial Cells
Product Information
Cat. No. Product Description
CC-3170 BEGM® BulletKit® Bronchial Epithelial Cell Growth Medium BulletKit®, Serum-free
CC-3171 BEBM® Basal Medium Bronchial Epithelial Cell Basal Medium, Serum-free
CC-4175 BEGM® SingleQuots® Kit Formulates BEBM® to BEGM®
BPE, 2 ml; Hydrocortisone, 0.5 ml;
hEGF, 0.5 ml; Epinephrine, 0.5 ml;
Transferrin, 0.5 ml; Insulin, 0.5 ml;
Clonetics® Media
Triiodothyronine, 0.5 ml; GA-1000, 0.5 ml;

Retinoic Acid, 0.5 ml
CC-3118 SAGM™ BulletKit® Small Airway Epithelial Cell Growth Medium BulletKit®, Serum-free
CC-3119 SABM™ Basal Medium Small Airway Epithelial Cell Basal Medium, Serum-free
CC-4124 SAGM™ SingleQuots® Kit Formulates SABM™ to SAGM™
hEGF, 0.5 ml; Epinephrine, 0.5 ml;
Transferrin, 0.5 ml; Insulin, 0.5 ml;
Triiodothyronine, 0.5 ml; GA-1000, 0.5 ml;
Retinoic Acid, 0.5 ml; BSA-FAF, 5.0 ml
112
Endothelial Cell Media Options
Endothelial cell media are low serum media optimized EGM®-2 BulletKit®
for the proliferation of endothelial cells. Each component
— Refinements to basal medium and the growth factors
of the basal medium and each growth supplement is
carefully titered for optimal growth by Lonza’s R&D team. — Does not contain BBE
Lonza currently offers four Clonetics® Media for the growth — Final serum concentration is 2%
of endothelial cells allowing for desired performance
— Improved cell proliferation over EGM®
and formulation flexibility. When selecting a medium to
use, refer to specific medium recommendations or call — EGM®-2 can be used to grow all of Clonetics® Endothelial
Scientific Support for assistance. Cells except microvascular, coronary artery, umbilical
artery, and iliac artery
EGM® and EGM® BulletKit®
— Basal medium developed for normal human endothelial EGM®-2MV BulletKit®
cells in a low-serum environment — Developed for the enhanced growth of lung micro-
— EGM® is supplemented growth medium and includes vascular endothelial cells
an attached aliquot of Bovine Brain Extract (BBE) — Does not contain BBE
— EGM® BulletKit® includes basal medium with supple- — Final serum concentration increased to 5%
ments and growth factors in separate, frozen aliquots
— EGM®-2MV can be used to grow all Clonetics® Endothelial
— Final serum concentration is 2% Cells except HUVEC
Clonetics® Media
— EGM® can be used to grow all of Clonetics® Endothelial
Cells except microvascular, coronary artery, umbilical
artery, and iliac artery
EGM® MV BulletKit®
— Developed for microvascular and coronary artery
endothelial cells
— Same basal medium as in EGM®
— Final serum concentration is 5%
— EGM®-MV can be used to grow most Clonetics®
Endothelial Cells
113
Endothelial Cell Media Options
Continued
Product Information
CC-3125 EGM® MV BulletKit® Microvascular Endothelial Cell Growth Medium BulletKit® w/ 5% FBS
CC-3121 EBM® Basal Medium Endothelial Cell Basal Medium, Serum-free
CC-4143 EGM® MV SingleQuots® kit Formulates EBM® to EGM® MV
BBE, 2 ml; hEGF, 0.5 ml;
Hydrocortisone, 0.5 ml; FBS, 25 ml; GA-1000, 0.5 ml
CC-3202 EGM®-2 MV BulletKit® Microvascular Endothelial Cell Growth Medium-2 BulletKit® w/ 5% FBS
CC-3156 EBM®-2 Basal Medium-2 Endothelial Cell Basal Medium-2, Serum-free
CC-4147 EGM®-2 MV SingleQuots® Kit Formulates EBM®-2 to EGM®-2 MV
hEGF, 0.5 ml; Hydrocortisone, 0.2 ml; FBS, 25 ml;
VEGF, 0.5 ml; hFGF-B, 2 ml; R3-IGF-1, 0.5 ml;
Ascorbic Acid, 0.5 ml; GA-1000, 0.5 ml
(No BBE)
CC-3024 EGM® Complete Medium Endothelial Cell Growth Medium w/ 2% FBS
CC-3124 EGM® BulletKit® Endothelial Cell Growth Medium BulletKit® w/ 2% FBS
CC-3121 EBM® Basal Medium Endothelial Cell Basal Medium, Serum-free
CC-3129 EBM®-PRF EBM® w/o Phenol Red
CC-4133 EGM® SingleQuots® Kit Formulates EBM® to EGM®
BBE, 2 ml; hEGF, 0.5 ml;
Hydrocortisone, 0.5 ml; FBS, 10 ml; GA-1000, 0.5 ml
CC-3162 EGM®-2 BulletKit® Endothelial Cell Growth Medium-2 BulletKit® w/ 2% FBS
CC-3156 EBM®-2 Basal Medium-2 Endothelial Cell Basal Medium-2, Serum-free
CC-4176 EGM®-2 SingleQuots® Kit Formulates EBM®-2 to EGM®-2
hEGF, 0.5 ml; Hydrocortisone, 0.2 ml; FBS, 10 ml;
Clonetics® Media
VEGF, 0.5 ml; hFGF-B, 2 ml; R3-IGF-1, 0.5 ml;

Ascorbic Acid, 0.5 ml; GA-1000, 0.5 ml, Heparin, 0.5 ml
(No BBE)
114
Fibroblast Cell Media Options
Fibroblast media has been optimized for the proliferation of FGM® BulletKit®
fibroblasts. Each component of the basal medium and each
— FGM® is a defined medium system and does not contain
growth supplement is carefully titered for optimal growth
serum
by Lonza’s R&D team. Lonza currently offers two media
choices for the growth of fibroblasts, allowing for desired
performance and formulation flexibility. When selecting a FGM®-2 BulletKit®
medium to use, refer to specific media recommendations
— Contains a vial of FBS for a final serum concentration
or call Scientific Support for assistance.
of 2%
Product Information
CC-3132 FGM®-2 BulletKit® Fibroblast Growth Medium BulletKit®-2, w/ 2% FBS
CC-3131 FBM® Basal Medium Fibroblast Basal Medium
CC-4126 FGM® SingleQuots® Kit Formulates FBM® to FGM-2
hFGF-B, 0.5 ml; Insulin, 0.5 ml FBS, 10 ml;
GA-1000, 0.5 ml
CC-3130 FGM® BulletKit® Fibroblast Growth Medium BulletKit®, Defined
CC-3131 FBM® Basal Medium Fibroblast Basal Medium
CC-4134 FGM® SingleQuots® Kit Formulates FBM® to FGM
hFGF-B, 0.5 ml; Insulin, 0.5 ml; GA-1000, 0.5 ml
Clonetics® Media
Hepatocyte Media
Product Information
CC-3198 HCM™ BulletKit® Hepatocyte Culture Medium, Phenol red-free
CC-3199 HBM™ Basal Medium Hepatocyte Basal Medium, Phenol red-free, Serum-free
CC-4182 HCM™ SingleQuots® Kit Formulates HBM™ to HCM™
hEGF, 0.5 ml; Transferrin 0.5 ml; Hydrocortisone, 0.5 ml;
BSA, 10.0 ml; Ascorbic Acid 0.5 ml; GA-1000, 0.5 ml, Insulin, 0.5 ml
CC-3197 HMM™ Medium HMM™, Hepatocyte Maintenance Medium, Phenol red-free, Serum-free
CC-4192 HMM™ SingleQuots® Kit HMM™ SingleQuots®, required supplements for use with HMM™
to provide optimal maintenance of cells, Insulin;
0.5 ml Dexamethasone, 0.5 ml; GA-1000, 0.5 ml
115
Keratinocyte Cell Media Options
Keratinocyte media has been optimized for the proliferation KGM®-2 BulletKit®
of keratinocytes. Each component of the basal medium
— Refinements to the basal medium and the growth
and each growth supplement is carefully titered for optimal
factors
growth by Lonza’s R&D team. Lonza currently offers three
media choices for the growth of keratinocytes, allowing — Grows normal human keratinocytes up to 20% faster
for desired performance and formulation flexibility. and allows for additional population doublings when
When selecting a medium to use, refer to specific media compared to KGM® and other commercially available
recommendations in the cell systems sections or call your keratinocyte media
Scientific Support for assistance. — KGM®-2 can be used to grow all Clonetics® NHEK Normal
Human Epidermal Keratinocytes
KGM® and KGM® BulletKit®
KGM®-CD
— Optimized for Clonetics® NHEK – Normal Human
Epidermal Keratinocytes in a serum-free environment — Supports isolation and proliferation of primary human
— KGM® includes supplemented medium and an attached keratinocytes in culture
aliquot of Bovine Pituitary Extract (BPE) — Chemically defined – No serum and no animal or plant
— KGM® BulletKit® includes basal medium with all extracts
supplements and growth factors in separate, frozen — Minimized variable experimental results due to
aliquots unknown effects of animal or plant-derived
Clonetics® Media
— KGM® can be used to grow all Clonetics® NHEK Normal components

Human Epidermal Keratinocytes — Obtain cleaner and more accurate results quickly
Product Information
CC-3107 KGM®-2 BulletKit® Keratinocyte Growth Medium-2 BulletKit®, Serum-free
CC-3103 KBM®-2 Basal Medium-2 Keratinocyte Basal Medium-2
CC-3108 KGM®-2 w/o Ca++ BulletKit® Keratinocyte Growth Medium-2 BulletKit®, Calcium-Free, Serum-free
CC-3158 KBM®-2 w/o Ca++ Keratinocyte Basal Medium-2, Calcium-Free, Serum-free
CC-4152 KGM®-2 SingleQuots® Kit Formulates KBM®-2 to KGM®-2
BPE, 2 ml; hEGF, 0.5 ml; Insulin, 0.5 ml
Hydrocortisone, 0.5 ml; Epinephrine, 0.5 ml; Transferrin, 0.5 ml;
GA-1000, 0.5 ml
CC-3001 KGM® Complete Medium Keratinocyte Growth Medium, Serum-free
CC-3111 KGM® BulletKit® Keratinocyte Growth Medium BulletKit®, Serum-free
CC-3101 KBM® Basal Medium Keratinocyte Basal Medium, Serum-free
CC-3112 KGM® BulletKit® w/o Ca++ Keratinocyte Growth Medium BulletKit®, Calcium-free, Serum-free
CC-3104 KBM® w/o Ca++ Keratinocyte Basal Medium, Calcium-free, Serum-free
CC-4131 KGM® SingleQuots® Kit Formulates KBM® to KGM®
BPE, 2 ml; hEGF, 0.5 ml; Insulin, 0.5 ml;
Hydrocortisone, 0.5 ml; GA-1000, 0.5 ml
CC-4455 KGM®-CD BulletKit® Keratinocyte Growth Media – Serum-free, Non-animal origin components
CC-3255 KBM®-CD Basal Medium Keratinocyte Basal Media – Chemically Defined
CC-4456 KGM®-CD SingleQuots® Kit Formulates KBM®-CD to KGM®-CD
Recombinant human insulin 1 ml; growth supplement, 5 ml
116
Mammary Epithelial Cell Media, Serum-free
Product Information
CC-3051 MEGM® Complete Medium Mammary Epithelial Cell Growth Medium, Serum-free, complete medium
CC-3150 MEGM® BulletKit® Mammary Epithelial Cell Growth Medium BulletKit®, Serum-free
CC-3151 MEBM® Basal Medium Mammary Epithelial Cell Basal Medium, Serum-free
CC-3153 MEBM®-PF free Mammary Epithelial Cell Basal Medium Phenol Red Free, Serum-free
CC-3152 MEBM®-Bicarb free Mammary Epithelial Cell Basal Medium Sodium Bicarbonate Free, Serum-free
CC-4136 MEGM® SingleQuots® Kit Formulates MEBM® to MEGM®
hEGF, 0.5 ml; Insulin, 0.5 ml; GA-1000, 0.5 ml
Melanocyte Cell Medium, Serum-free
Clonetics® Melanocyte Cell Medium has been optimized MGM-4

for the growth and proliferation of normal human primary
Clonetics® Media
Melanocytes in culture. This medium system has been — Melanocyte Growth Media has been qualified and
shown to deliver superior results as compared to other tested together to provide optimum performance
existing commercial media systems. Melanocytes in culture
maintain >90% functionality based on the conversion of — The media system is offered as BulletKit® (basal
L-dopa to dopa-melanin. The Melanocyte Media system medium and separately packaged growth factors) to
also allows for normal morphology and proliferative capacity allow for flexibility with your research project
after recovery from cryopreservation and throughout
serial passaging. — Adult melanocytes also require the addition of Et-3,
sold separately
Product Information
CC-3249 MGM® BulletKit® Melanocyte Growth Medium
CC-3250 MBM®-4 Basal Medium-4 Melanocyte Basal Medium, Serum-free
CC-4435 MGM®-4 SingleQuots® Kit Formulates MBM®-4 to MGM®-4
CaCl2, 1.0 ml; hFGF-B, 1.0 ml; PMA, 0.5 ml; rhInsulin, 1.0 ml;
Hydrocortisone, 0.5 ml; BPE, 2.0 ml; FBS, 2.5 ml;
Gentamicin/Amphotericin B, 0.5 ml
Neural Cell Medium, Low Serum
Product Information
CC-3186 AGM™ BulletKit® Astrocyte Growth Medium BulletKit®, w/3.0% FBS
CC-3187 ABM™ Basal Medium Astrocyte Basal Medium, Serum-free, without L-glutamine
CC-4123 AGM™ SingleQuots® Kit Formulates ABM™, to AGM™
Insulin, 1.25 ml; rhEGF, 0.5 ml;
FBS, 15.0 ml; Ascorbic Acid, 0.5 ml;
L-glutamine, 5.0 ml; GA-1000, 0.5 ml
117
Prostate Epithelial Cell Medium, Serum-free
Product Information
CC-3166 PrEGM™ BulletKit® Prostate Epithelial Cell Growth Medium BulletKit®, Serum-free
CC-3165 PrEBM™ Basal Medium Prostate Epithelial Cell Basal Medium, Serum-free
CC-4177 PrEGM™ SingleQuots® Kit Formulates PrEBM® to PrEGM®
BPE, 2.0 ml; Hydrocortisone, 0.5 ml; hEGF, 0.5 ml;
Epinephrine, 0.5 ml; Transferrin, 0.5 ml; Insulin, 0.5 ml;
Retinoic Acid, 0.5 ml; Triiodothyronine, 0.5 ml;
GA-1000, 0.5 ml
Renal Cell Media, Low Serum
Product Information
Clonetics® Media

CC-3190 REGM™ BulletKit® Renal Epithelial Cell Growth Medium BulletKit®, w/ 0.5% FBS
CC-3191 REBM™ Basal Medium Renal Epithelial Cell Basal Medium, Serum-free
CC-4127 REGM™ SingleQuots® Kit Formulates REBM® to REGM®
Hydrocortisone, 0.5 ml; hEGF, 0.5 ml;
FBS, 2.5 ml; Epinephrine, 0.5 ml;
Triiodothyronine, 0.5 ml; Transferrin, 0.5 ml;
Insulin, 0.5 ml; GA-1000, 0.5 ml
CC-3146 MsGM™ BulletKit® Mesangial Cell Growth Medium BulletKit®, w/ 5% FBS
CC-3147 MsBM™ Basal Medium Mesangial Cell Basal Medium, Serum-free
CC-4146 MsGM™ SingleQuots® Kit Formulates MsBM™ to MsGM™
FBS, 25 ml; GA-1000, 0.5 ml
Primary Neuron Growth Medium, Serum-free
Product Information
CC-4461 PNGM™ BulletKit® Primary Neuron Growth Medium BulletKit®
CC-3256 PNBM™ Basal Medium Primary Neuron Basal Medium
CC-4462 PNGM™ SingleQuots® Kit Formulates PNBM to PNGM™
NSF-1, 4 ml; L-glutamine, 2 ml; GA-1000, 0.2 ml
CC-4512 PNGM™-A BulletKit® Primary Neuron Growth Media-Adult
CC-4511 PNGM™-A SingleQuots® Kit Formulates PNBM to PNGM™-Adult
NSF-1, 4 ml; L-glutamine, 2 ml; Pen/Strep, 2 ml
118
Skeletal Cell Media
Product Information
CC-3207 OGM™ BulletKit® Osteoblast Growth Medium BulletKit®, w/ 10% FBS
CC-3208 OGM™ Basal Medium Osteoblast Cell Basal Medium, Serum-free
CC-4193 OGM™ SingleQuots® Kit Formulates OBM™ to OGM™
FBS, 50 ml; Ascorbic Acid, 0.5 ml; GA-1000, 0.5 ml
CC-4194 OGM™ Differentiation SingleQuots® Kit Induces osteoblast differentiation and bone mineralization.
Hydrocortisone-21-Hemisuccinate, 0.5 ml;
β-Glycerophosphate, 5.0 ml
CC-3216 CGM™ BulletKit® Chondrocyte Growth Medium BulletKit®
CC-3217 CBM Basal Medium Chondrocyte Basal Medium
CC-4409 CGM™ SingleQuots® Kit Formulates CBM to CGM™
R3-IGF-1, 1.0 ml; bFGF, 2.5 ml; Insulin, 1.0 ml;
Transferrin, 0.5 ml; FBS, 25 ml;
Gentamicin/Amphotericin-B, 0.5 ml
CC-3225 CDM™ BulletKit® Chondrocyte Differentiation Medium BulletKit®, Serum-free
CC-3226 CDM™ Basal Medium Chondrocyte Differentiation Basal Medium
CC-4408 CDM™ SingleQuots® Kit Formulates CDM™ Basal Medium to CDM™ Differentiation Medium
TGF-β, 1.25 ml; R3-IGF-1, 0.5 ml; Insulin, 0.5 ml;
Transferrin, 0.5 ml; FBS, 12.5 ml; GA-1000, 0.25 ml
Clonetics® Media
Skeletal Muscle Cell Media
Product Information
CC-3160 SkGM® BulletKit® Skeletal Muscle Growth Medium BulletKit®, Serum-free
CC-3161 SkBM® Basal Medium Skeletal Muscle Basal Medium, Serum-free
CC-4139 SkGM® SingleQuots® Kit Formulates SKBM® to SKGM®
hEGF, 0.5 ml; Insulin, 5 ml;
BSA, 5 ml; Fetuin, 5 ml;
Dexamethasone, 0.5 ml; GA-1000, 0.5 ml
CC-3245 SkGM®-2 BulletKit® Skeletal Muscle Myoblast Growth Medium-2 BulletKit®, with 10% FBS
CC-3246 SKBM®-2 Basal Medium-2 Skeletal Muscle Myoblast Basal Medium-2, Serum-free
no L-glutamine
CC-3244 SkGM®-2 SingleQuots® Kit Formulates SKBM®-2 to SKGM®-2
FBS, 50.0 ml; GA-1000, 0.5 ml; rhEGF, 0.5 ml;
Dexamethasone, 0.5 ml; L-glutamine,10.0 ml
119
Smooth Muscle Cell Medium
Smooth muscle medium has been optimized for the SmGM®-2 BulletKit®
proliferation of smooth muscle cells. Each component
— SmGM®-2 BulletKit® was developed for better cell
of the basal medium and each growth supplement is
morphology and proliferation
carefully titered for optimal growth by Lonza’s R&D team.
— Final serum concentration is 5%
— SmGM®-2 can be used to grow all smooth muscle cells
Product Information
CC-3182 SmGM®-2 BulletKit® Smooth Muscle Growth Medium-2 BulletKit® w/ 5% FBS
CC-3181 SmBM® Basal Medium Smooth Muscle Basal Medium, Serum-free
CC-4149 SmGM®-2 SingleQuots® Kit Formulates SmBM® to SmGM®-2
hEGF, 0.5 ml; Insulin, 0.5 ml;
hFGF- B, 1 ml; FBS, 25 ml; GA-1000, 0. 5 ml
Clonetics® Media
Stromal Cell Medium, Low Serum
Product Information
CC-3205 SCGM™ BulletKit® Stromal Cell Growth Medium BulletKit®, w/ 5% FBS
CC-3204 SCBM™ Basal Medium Stromal Basal Medium, w/o phenol red, Serum-free
CC-4181 SCGM™ SingleQuots® Kit Formulates SCBM™ to SCGM™
hFGF-B, 0.5 ml; Insulin 0.5 ml;
FBS, 25 ml; GA-1000, 0.5 ml
120
Poietics® FAQs
Media
Q. What is a BulletKit®? Q. I don’t see the specific cell type I want in your
catalog or on your website, do you do custom
A. A BulletKit® contains basal medium and
SingleQuots® Kit of supplements and growth factors. isolations?
In most cases, it is possible to purchase each of A. Yes, Lonza offers custom isolations and formats
these components separately. Once the basal for various types of non-catalog cell products. See
medium is supplemented with the SingleQuots® page 79 & 98 for additional information.
Kit, it becomes a complete growth or differentiation
medium. Q. Can I expand the mononuclear cells, CD34+
progenitors, or the CD133+ cells without
Q. What are SingleQuots® Supplements and Growth
differentiating them?
Factors?
No, the cells will proliferate and differentiate
A. SingleQuots® Supplement and Growth Factors
simultaneously upon being placed in culture.
contains several vials of growth factors,
Varying the cytokine cocktail can influence the
supplements and antibiotics in premeasured
differentiation and proliferation of the cells. Please
aliquots designed to be added to a bottle of
contact Scientific Support for more information.
basal medium. Serum will be provided in the
SingleQuots® Kit if it is to be added to the Q. Can I expand the human mesenchymal stem
basal medium. The concentrations of these growth cells prior to differentiating them?
factors are proprietary.
A. These cells are frozen in 2nd passage and we
Q. What media should I use for Poietics® Cells? recommend they are used before passage 5.
Poietics® FAQs
A. Information regarding specific media selections Q. Can I expand the preadipocytes prior to differ-
can be found in the Technical Data Sheet or the entiating them?
Instructions. Please contact Scientific Support for
more information. A. The subcutaneous cells can be passaged twice
prior to differentiation. The visceral cells can be
Q. What is the difference between the different cell passaged once prior to being differentiated.
sources: bone marrow, cord blood, or fetal liver?
Q. Can I get subcutaneous and visceral preadipocytes
A. Bone Marrow: provides a hematopoietic micro- from the same donor?
environment and the most number of different
donors. A. Yes, please contact Scientific Support for more
Cord Blood: more naïve cells useful in transplant information.
and stem cell research.
Fetal Liver: best for developmental studies and they
are highly proliferative.
Q. Are the fresh cells you provide equivalent to their
cryopreserved counterparts?
A. Yes, the cells perform equivalently by all
measures we have tested. Some cells do die in the
cryopreservation and thawing processes, but it
does not seem to be selective to a particular cell
type. We put additional cells in each vial to account
for this loss.
121
Procedure for Thawing CD34+ and Precursor Cells
1. Warm medium containing 10% FBS or 1% BSA. For 9. Slowly bring the volume up to fill the tube by adding
mononuclear cells and hematopoietic progenitors, 1 ml to 2 ml volumes of medium while gently
DNase I (20 U/ml) should also be added.* swirling after each addition of medium.
2. Quickly thaw the vial of frozen cells in a 37°C water 10. Centrifuge the cell suspension at 200 × g at room
bath. Wipe the outside of the vial with 70% ethanol. temperature for 15 minutes.
3. Aseptically transfer a maximum of 2 ml of cell 11. Carefully remove by pipette all but 2 ml of the wash.
suspension to a 50 ml conical tube. For one million Gently resuspend the cell pellet in the remaining 2
cells or less, use a 15 ml conical tube. ml of medium and count. If cell count is lower than
4. Rinse the vial with 1 ml of medium. Add the rinse expected, centrifuge the wash saved in step 8 at a
dropwise to the cells while gently swirling the tube higher speed, count and combine if necessary.
(≈1 minute). 12. Rest the cells for 1 hour at 37°C and 5% CO2. Count
5. Slowly add enough medium dropwise to the cells the cells a second time. The cells are ready to be
until the total volume is 5 ml, while gently swirling put in culture.
*For the addition of DNase, prepare 20 ml of medium
after each addition of several drops of medium containing 10% FBS and 20 U/ml of DNase I (Sigma D 4513).
(≈3 minutes). Proceed as above, using the DNase-containing medium to
dilute the cells. Centrifuge the cells and continue with step #8.
6. Slowly bring the volume up to fill the tube by adding
1 ml to 2 ml volumes of medium dropwise, while
gently swirling after each addition of medium

(≈5 – 10 minutes).
7. Centrifuge the cell suspension at 200 × g at room
Poietics®
temperature for 15 minutes.

8. Carefully remove by pipette (and save in a second
tube) most of the wash, leaving a few mls behind
so the cell pellet is not disturbed. Gently resuspend
the cell pellet in the remaining medium. If you are
using a 50 ml tube, transfer the cells to a 15 ml
conical tube and rinse the 50 ml tube with 5 ml of
medium. Slowly add the 5 ml wash medium to the
cell suspension with gentle swirling.
122
Human Mesenchymal Stem Cell Media
Product Information
PT-3001 MSCGM™ BulletKit® Mesenchymal Stem Cell Growth Medium BulletKit®
PT-3238 MSCGM™ Basal Medium Mesenchymal Stem Cell Basal Medium
PT-4105 MSCGM™ SingleQuots® Kit Formulates MSCBM to MSCGM™ Growth Medium
MCGS, 50 ml; L-glutamine, 10 ml; GA-1000, 0.5 ml
PT-3002 hMSC Differentiation Kit– Osteogenic Mesenchymal Stem Cell Differentiation Kit – Osteogenic
PT-3924 hMSCBM – Osteogenic Basal Medium Mesenchymal Stem Cell Basal Medium – Osteogenic
PT-4120 hMSC SingleQuots® Kit – Osteogenic Formulates Osteogenic Basal Medium to Osteogenic Differentiation Medium
Dexamethasone, 1 ml; β-Glycerophosphate, 2 ml; Ascorbate, 1 ml;
Penicillin /Streptomycin, 2 ml; MCGS, 20 ml; L-glutamine, 4 ml
PT-3003 hMSC Differentiation Kit – Chondrogenic Mesenchymal Stem Cell Differentiation Kit – Chondrogenic
PT-3925 hMSCBM – Chondrogenic Basal Medium Mesenchymal Stem Cell Basal Medium – Chondrogenic
PT-4121 hMSC SingleQuots® Kit – Chondrogenic Formulates Chondrogenic Basal Medium to Chondrogenic Differentiation Medium
ITS+, 2 ml; Sodium Pyruvate, 2 ml; Proline, 2 ml; Dexamethasone, 1 ml;
Ascorbate, 2 ml; GA-1000, 0.2 ml; L-glutamine, 4 ml
PT-4124 TGF-β3 Required component sold separately
PT-3004 hMSC Differentiation Kit – Adipogenic Mesenchymal Stem Cell Differentiation Kit – Adipogenic
PT-3102A hMSC Adipogenic Maintenance Medium Mesenchymal Stem Cell Maintenance Medium – Adipogenic
PT-3102B hMSC Adipogenic Induction Medium Mesenchymal Stem Cell Induction Medium – Adipogenic
PT-4122 hMSC Maintenance SingleQuots® Kit – Adipogenic Formulates Adipogenic MM to Adipogenic Differentiation Medium
rhInsulin, 2 ml; GA-1000, 0.2 ml; MCGS, 20 ml; L-glutamine, 4 ml
Poietics® Media
PT-4135 hMSC Induction SingleQuots® Kit – Adipogenic Formulates Adipogenic IM to Adipogenic Differentiation Medium
Indomethacin, 0.4 ml; IBMX, 0.2 ml; rhInsulin, 2 ml; Dexamethasone, 1 ml;
GA-1000, 0.2 ml; MCGS, 20 ml; L-glutamine, 4 ml
Preadipocyte Growth Medium
Product Information
PT-8002 PGM™-2 BulletKit® Preadipocyte Growth Medium-2 BulletKit®
PT-8202 PBM-2 Basal Medium-2 Preadipocyte Basal Medium-2
PT-9502 PGM™-2 SingleQuots® Kit Formulates PBM-2 to PGM™-2 FBS (10%), 50 ml; L-Glutamine, 5 ml; GA-
1000, 0.2 ml, 5 ml; rhInsulin, 2 ml; Dexamethasone, 0.2 ml; Indomethacin,
0.4 ml; IBMX, 0.2 ml
123
Osteoclast Growth Medium
Product Information
PT-8001 Osteoclast Growth Medium BulletKit® Osteoclast Growth Medium BulletKit®
PT-8201 OBM™ Osteoclast Basal Medium
PT-9501 Osteoclast Growth Medium SingleQuots® Kit Formulates OBM™ to Osteoclast Growth Medium FBS (10%), 10 ml;
L-glutamine, 1 ml; Penicillin/Streptomycin, 1 ml; M-CSF, 0.1 ml;
Soluble RANK Ligand, 2 μg
Neural Progenitor Growth Medium
Product Information
CC-3209 NPMM™ BulletKit® Neural Progenitor Maintenance Medium BulletKit®
Poietics® Media
CC-3210 NPBM™ Basal Medium Neural Progenitor Basal Medium

CC-4242 NPDM SingleQuots® Kit Formulates NPBM™ to NPMM™ (for differentiation) NSF-1, 4 ml;
GA-1000, 0.4 ml
CC-4241 NPMM™ SingleQuots® Kit Formulates NPBM™ to NPMM™ (for maintenance) rhEGF, 0.4 ml;
rhFGF, 0.4 ml
CC-3229 NPDM BulletKit® Neural Progenitor Differentiation Medium BulletKit®
CC-3210 NPBM™ Basal Medium Neural Progenitor Basal Medium
CC-4242 NPDM SingleQuots® Kit Formulates NPBM™ to NPDM (for differentiation) NSF-1, 4 ml;
GA-1000, 0.4 ml
124
Amaxa® Nucleofection®
and Transfection
Chapter 2
and Transfection
Nucleofection®
Amaxa® Nucleofection® Antibiotics and Antimycotics

Introduction 127 MycoZap™ Mycoplasma Elimination Reagent 198
Amaxa® Nucleofector® Technology 128 – 131 Antibiotics and Antimycotics 199
Nucleofector® II Device 132
96-well Shuttle® Device 133 Other Transfection Products
Nucleofector® Extended Guarantee and
Service Contracts 134 siRNA Test Kit 200
96-well Shuttle® Automation Package 135 – 137 Amaxa® Peptide Transfection Control 201
List of Nucleofector® Kits for Primary Cells 138 – 139
Nucleofector® Kits for Blood Cells 140 – 150
Nucleofector® Kits for Dermal Cells 151 – 155
Nucleofector® Kits for Endothelial Cells 156 – 158
Nucleofector® Kits for Epithelial Cells 159 – 163
Nucleofector® Kits for Hepatocytes 164 – 165
Nucleofector® Kits for Neural Cells 166 – 171
Nucleofector® Kits for Smooth Muscle Cells 172 – 173
Nucleofector® Kits for Stem Cells 174 – 179
Nucleofector® Kits for Rat Cardiomyocytes 180
Nucleofector® Kits for Human Chondrocytes 181
Nucleofector® Kits for
Mouse Embryonic Fibroblasts (MEF) 182
List of Optimized Protocols for Cell Lines 183 – 184
Cell Line Nucleofector® Kits 185
Cell Line Optimization Nucleofector® Kits 186 – 187
cGMP Cell Line Nucleofector® Kits 188
Basic Parasite Nucleofector® Kits 188
FAQs on Nucleofection® 189 – 190
Transfection Reagents
HiFect® Transfection Reagent 191
Transport™ Protein Delivery Reagent 202
PrimeFect™ Transfection Reagent 203 & 204
FAQs Transport Reagent 205
Vectors
pmaxFP® Fluorescent Protein Expression Vectors 192
pmaxFP® Vector Backbone Information 197
pmaxFP®-Green Vectors 198
pmaxFP®-Yellow Vectors 199
pmaxFP®-Red Vectors 200
pmaxCloning™ Vector 201
126
49(0)221-99199-400 www.lonza.com/research
and Transfection
Nucleofection®
Selecting the Ideal Technology and Product
for your Needs
Lonza provides comprehensive information and protocols
regarding the introduction of many bio-molecules and
particles. This information and more, is available online
at www.lonza.com/celldatabase or through Scientific
Support. Nucleofector® Devices and Kits are available
in both single sample and 96-well formats to suit your
individual throughput needs and to ensure that you have
continuity of transfection method as your processing
capacity increases over time. The selection chart below,
and others hosted on the Lonza website, are designed to
help you quickly focus your transfection methodologies.
Identify the right methodology based on your cell type and substrate
Substrate
DNA RNA Protein Other
Plasmid DNA, cDNAs, siRNA, shRNA, Peptide, Protein Morpholinos, pNAs, etc.
Cell type Gene constructs miRNA
Primary cells Nucleofector® Device Nucleofector® Device Nucleofector® Device Nucleofector® Device
96-well Shuttle® System 96-well Shuttle® System 96-well Shuttle® System 96-well Shuttle® System
Difficult-to-transfect Nucleofector® Device Nucleofector® Device Nucleofector® Device Nucleofector® Device

cell lines 96-well Shuttle® System 96-well Shuttle® System 96-well Shuttle® System 96-well Shuttle® System
Standard cell lines HiFect® Reagent HiFect® Reagent Nucleofector® Device Nucleofector® Device
Nucleofector® Device Nucleofector® Device 96-well Shuttle® System 96-well Shuttle® System
96-well Shuttle® System 96-well Shuttle® System
Contact Lonza Scientific Support to review your selection and discover more about the solution that fits your needs!
Scientific Support
Europe/World USA
– Phone: +49(0)221-99199-400 – Phone: 888-632-9110
– Fax: +49(0)221-99199-499 – Fax: (888) 632-9112
– Email: scientific.support.eu@lonza.com – Email: scientific.support@lonza.com
127
Amaxa® Nucleofector® Technology
Continued
Nucleofector®
Technology
Amaxa® Nucleofector® Technology and into the nucleus. Transfected cells retain excellent
viability and the function of intracellular systems is
The application of systems biology and multidisciplinary
highly conserved. Whatever your application, Amaxa®
approaches require that cells and model systems display
Transfection Specialists are available to assist you in
’in-vivo-like’ cellular functionality. This means that the
rapidly optimizing your new application.
future of cell transfection is in using primary cell types, and
that transfecting these physiologically relevant cell types
One technology – the broadest choice of cell types
presents researchers with a series of technical challenges.
Using the Nucleofector® Technology, cell lines, as well
The most physiologically relevant cell types are typically
as primary cells, can be reliably transfected at high
the most difficult to transfect using traditional methods. In
efficiency. Delivery of nucleic acid substrates directly into
contrast, when using relevant cell lines as model systems,
both the nucleus and cytoplasm ensures transfection
the critical issues are to achieve reproducibly efficient
efficiencies of up to 99% in both cell lines and primary
transfection with high levels of viability while matching
cells. More than 200 ready-to-use Amaxa® Optimized
throughput capability with the number of transfections
Protocols contain cell-type specific guidance and Lonza's
required at each project phase - from proof of concept,
Amaxa® Cell Database contains user-developed methods
through scale-up and screening-like applications.
and data for more than 1300 cell types.
DNA delivery straight into the nucleus

A B
Structural Genomics
Expression
Plasmid Pre-
miRNA
shRNA
System Biology
DNA Antisense
Oligos
Functional
Antibodies Genomics
Peptide siRNA
Protein
C D RNA
Protein
Small miRNA
Molecules
Drug mRNA
Candidates
Fluoresent
conjugates
Drug Discovery
Normal human dermal fibroblasts - neonatal were transfected with 2.5 µg Substrate flexibility – explore the limits of your
TMR-labeled plasmid DNA encoding eGFP. After 2 hours, cells were fixed
with 3.5% PFA and analyzed by confocal microscopy. TMR label is shown in
imagination
(A), GFP fluorescence in (B), DAPI nuclear staining in (C) and a merge of all The flexibility that Nucleofection® allows in planning
three fluorescent labels in (D).
experimental projects stems from Lonza's proven
capability to transfect the widest range of substrate
Amaxa® Nucleofection® – your unique advantage molecules into the widest range of cell types. Imagine
Amaxa® Nucleofection® is a proprietary technology the flexibility to first complement a knock-out mutant by
based on the momentary creation of small pores in transfecting a DNA vector, then to explore the regulation
cell membranes by applying an electrical pulse. The of the gene(s) of interest using multiple or single shRNA,
comprehensive way in which Nucleofector® Pulse siRNA, or miRNA substrates using the same Nucleofector®
Conditions and cell-type specific solutions are developed Kit and conditions.
ensures that nucleic acid substrates are delivered not only
Reflecting the focus of molecular biologists, the flexibility
to the cytoplasm, but also through the nuclear membrane
of Nucleofection® now extends beyond nucleic acid
128
Continued
Nucleofector®
Technology
substrates, to include peptides, proteins, and antibodies. The Nucleofector® II Device and 96-well Shuttle® System
This allows yet further characterization and validation of Both the Nucleofector® Device and 96-well Shuttle® System
the cellular functions that interest your research group, deliver unique electrical parameters that are different from
e.g., allowing researchers to identify and characterize other commercially available electroporation instruments.
related signaling or protein modification pathways Electrical settings are pre-programmed for each optimized
using inhibitory peptides, before tracking active protein cell type and can be selected using the Nucleofector®
trafficking, or localization of active target molecules using Device or the PC-based software controlling the
antibody-conjugated nano-fluorophores or quantum-dots. 96-well Shuttle® Device.
The Nucleofector® and 96-well Shuttle® Kits

Two platforms – fulfilling your choice of sample Kits for Nucleofector® Technology contain Amaxa® speci-
throughputs fied plasticware, pipettes, pmaxGFP® Vector, Nucleofector®
The use of Nucleofector® Technology allows your trans- Solution and Supplement. Each solution and supplement
fection capabilities to grow in pace with your group, to is individually developed for every primary cell type. For
adapt to changing applications that require different cell lines, a set of different Nucleofector® Solutions is
levels of sample throughput, to accelerate the pace of available. All solutions provide a protective environment
your research, or to transfect only the number of cells that ensures the highest transfection efficiency and cell
that fits your unique application. viability, while helping maintain physiologically relevant
cellular functions.
Two platforms
Low throughput Low to high throughput

Device Nucleofector® Device 96-well Shuttle® System
Samples per run 1 1 - 96

Reaction volume 100 µl 20 µl
Cell number 2 x 10 to 2 x 10
5 7 5 x 104 to 1x 106
Substrate amount Oligonucleotides: Oligonucleotides:
0.2 - 200 pmol (2 nM - 2 µM) 0.04 - 40 pmol (2 nM - 2 µM)
Vector DNA: Vector DNA:

1 - 5 µM 1 - 5 µM
The Components of the Nucleofector® Technology

Proven – in leading labs
The Nucleofector® Technology is an established and
trusted transfection method in opinion-leading labs Cell Line Optimization Kits provide the ideal tools to
worldwide. Several thousand peer-reviewed publications conveniently determine the optimal Nucleofection®
illustrate the importance of Nucleofection® in hundreds parameters of your cell line of interest within a single
of leading-edge research applications using primary experiment.
cells, such as neurons and stem cells, as well as difficult-
to-transfect and standard cell lines.
129
Continued
Nucleofector®
Technology
Widest Range of Substrates
siRNA-mediated depletion of vimentin in human T cells

120
Relative Expression (% pulse only, control sample C)
100
50
40
30
20
10
0 1 3 5 7 9 1 13 15 1
Knockdown on mRNA level measured by qRT-PCR. 15 samples compared to

control (C) set to 100%.
(Data kindly provided by C. Merz, Bayer Schering Pharma AG, Berlin.)
Gene silencing in primary neurons by transfection of an shRNA vector – Quantitive down-regulation of CDC10 protein
A B C D E
2 100
CD C10 Signal (% of Calnexin Signal)

C10 Pum iPum2
s iCD mis s 80
CDC10 → 60
40
Calnexin → 20
0 siCDC10 siPum2 misPum2
Rat hippocampal neurons (E17) were transfected with the shRNA vector pSUPERIOR targeting CDC10 using the 96-well Shuttle® System. A and B: Efficient
Nucleofection® of pSUPERIOR is shown by eGFP expression after 1 day in vitro. C: Immunostaining of CDC10 (red fluorescence) shows reduced endogenous
CDC10 protein levels in transfected neurons (green) after 4 days in vitro compared to untransfected cells (red). Western blot analysis (D) and quantification (E)
of CDC10 down regulation.
(Data courtesy of Prof. Kiebler, Medical University of Vienna, Vienna, Austria.)
Transfection of Jurkat cells
Jurkat cells were transfected in the presence of a FAM-labeled octapeptide

(30 uM). Images were captured 2 hours post-transfection.
130
Continued
Nucleofector®
Technology
The Ideal Tool for Transfection of Cell Lines and Primary Cells
Cell Lines
Broad cell line coverage – From standard to difficult-to-transfect cells, including suspension cells
– More than 100 ready-to-use protocols
– Fast and easy optimization
High efficiencies – Up to 90% efficiency with plasmid DNA

– Up to 99% efficiency with siRNA duplexes
Reliable results – High viability and reproducibility
Safe and easy procedure – Easy and convenient handling
Fast experiments – Expression within hours – from transfection to analysis in one day
Primary Cells
High efficiencies – Up to 90% efficiency with plasmid DNA
– Up to 99% efficiency with siRNA duplexes
Reliable results – Reproducible high viability and functionality
No optimization – Ready-to-use kits for a multitude of cell types, e.g. neurons and stem cells
Reproducible results – Optimized Protocols for each cell type with details on cell isolation
and culture
Unique advantages – Direct transport of nucleic acids into the nucleus thus enabling transfection
even of non-dividing cells
Contact Lonza today regarding your unique application or to arrange to test the Nucleofector® Technology with your cells
and in your lab.
NOTE: The Nucleofector® Technology, comprising Nucleofection® Process,

Nucleofector® Device, Nucleofector® Solutions, Nucleofector® 96-well
Shuttle® System and Nucleocuvette® plates and modules is covered by
patent and/or patent pending rights owned by Lonza Cologne AG.
131
Nucleofector® II Device
Nucleofector®
Technology
The Nucleofector® II Device delivers electrical parameters Ordering Information

that differ from other commercially available electro- Cat. No. Price
poration instrument. Electrical settings are pre-programmed
for each optimized cell type, while an integrated chip-card Nucleofector® II Device
reader allows quick program updates for future applications. AAD-1001
In addition to programs for transfection of eukaryotic cells,
the Nucleofector® II Device program list (from version N5-5
S4-2 on) includes programs for bacteria transformation.
It also offers a language selection (English or Japanese).
Weighing only 2.8 kg (6.2 lb), the device is conveniently
sized so that it easily fits under a laminar-flow hood enabling
sterile work with cells.
Features of the Nucleofector® II Device

■ Easy handling
– Motor-driven carousel for automatic cuvette handling
■ High convenience
– Large graphic display for easy day-to-day use Technical Specifications
■ Great flexibility Dimensions (w x d x h) 30 x 23 x 11 cm (11.81 x 9.06 x 4.33 in)
Weight 2.8 kg (6.2 lb)
– Software enables the customized naming of
individual programs Power supply 100-110 VAC or 230 VAC
50-60 Hz, self-regulating
■ Future-proof Power consumption 50 VA/fuse T630mA L250V
– Program delivery unit for 96-well Shuttle® System Protection Class IP 20, EN 61010-1, UL 61010A-1
Clonetics®
132
96-well Shuttle® Device
96-well Shuttle®
Device
The 96-well Shuttle® Device is a high throughput extension Ordering Information
to the Nucleofector® II Device. The complete Nucleofector®
Cat. No. Price
96-well Shuttle® System consists of three components,
the 96-well Shuttle® Device, the Nucleofector® II Device 96-well Shuttle® Device
and a laptop computer. The Nucleofector® II Device serves AAM-1001
as the program delivery unit. The 96-well Shuttle® Device
including:
is the contacting unit which mediates the transfer of Laptop and 96-well Shuttle® Software
the respective 96-well program to a specific well of the The Nucleofector® II Device must be purchased independently
96-well Nucleocuvette® Plate. The interaction between
Nucleofector® II Device and Nucleofector® 96-well Shuttle®
Device is controlled via the 96-well Shuttle® Software
installed on the laptop.
Features of the 96-well Shuttle® Device

■ Transfection flexibility
– Modular 6 x 16 Nucleocuvette® Plate for
scalable throughput
– Variable cell numbers – from 104 – 106 cells
per reaction Technical Specifications
■ Rapid optimization Dimensions (w x d x h) 34 x 27 x 10 cm (13.39 x 10.63 x 3.94 in)
Weight 3.0 kg (6.6 lb)
– Up to 96 independent programs can be run per plate
Power supply 110 VAC +10%/-20% or 230 VAC +10%/-20%
– Optimization of any difficult-to-transfect cell line in 50-60 Hz, self-regulating
just one plate Power consumption 20 VA
Protection Class IP 22
■ Safety
– Disposable plates minimize the risk of cross
contamination Ordering Information
– Nucleocuvette® Plate with innovative conductive
Cat. No. Price
polymer electrodes – no metal ion release
96-well Shuttle® System Rack
■ HTS compatibility
AXA-1001
– Automatic processing of a 96-well plate
in 3 – 4 minutes
– Fulfills prerequisites for liquid handling integration Nucleofector® 96-well Shuttle® System
133
Nucleofector® Device Extended Guarantee and Service Contracts
Nucleofector® Device
Lonza provides an exceptional level of support for and Ordering Information

around the Nucleofector® Technology to guarantee
Cat. No. Price
high performance of our products and to ensure user
satisfaction. To better serve your needs, Lonza offers Nucleofector® Device Guarantee – 1 year Extension
the Nucleofector® Extended Guarantee and Service AWD-1001
Contracts that provides you with a number of benefits
(see below). The Nucleofector® Extended Guarantee has Nucleofector® Device Guarantee – 2 year Extension
to be purchased together with a Nucleofector® Device. AWD-1002
Service Contracts can be purchased at any time during a
guarantee period. Service Contract Nucleofector® Device Silver
AWA-1001
Contract Benefits
Service Contract Nucleofector® Device Gold
– 1 or 2 additional years of guarantee beyond the basic
AWA-2002
device guarantee of 1 year
– Diagnosis and repair of your Nucleofector® Device
including all replacement parts
– Costs for return shipments of repaired device
– Replacement of Nucleofector® Device within
5 business days*
*only for Gold Service Contracts
96-well Shuttle® Device Extended Guarantee and Service Contracts
Lonza provides the highest possible level of support to Ordering Information

insure continuous performance of the 96-well Shuttle® Cat. No. Price
Device. To further improve our service, Lonza offers
96-well Shuttle® Device Guarantee – 1 year Extension
the 96-well Shuttle® Extended Guarantee and Service
Contracts that helps you pre-plan your costs and keep AWM-1001
down-time as short as possible. The 96-well Shuttle®
96-well Shuttle® Device Guarantee – 2 year Extension
Extended Guarantee has to be purchased together with
the 96-well Shuttle® Device. Service Contracts can be AWM-1002
purchased at any time during a guarantee period. Service Contract 96-well Shuttle® Device Silver
Contract Benefits AWB-1001
– 1 or 2 additional years of guarantee beyond the basic Service Contract 96-well Shuttle® Device Gold
device guarantee of 1 year AWB-2002
– Diagnosis and repair of your 96-well Shuttle® Device
including all replacement parts
– Costs for return shipments of repaired device
– Replacement of 96-well Shuttle® Device within
5 business days*
*only for Gold Service Contracts
134
96-well Shuttle® Automation Package
Automation Package
96-well Shuttle®
Lonza’s 96-well Shuttle® Automation Package is the first Ordering Information
high throughput transfection device suitable for the
Cat. No. Size Price
transfection of difficult-to-transfect cell lines and primary
cells. For a complete automation of the Nucleofection® 96-well Shuttle® Automation Package
Process, the Nucleofector® 96-well Shuttle® System has SBA-1001
to be integrated into an automated environment providing
Software and Documentation:
liquid handling and plate handling capabilities such as – CD containing the 96-well Shuttle® Automation Software
Liquid Handling platforms from Tecan, Beckman Coulter, – Manual with detailed interface documentation
Hamilton, or other suppliers. For the integration, additional Support:
software is necessary to control the Nucleofection® – Support to LHS supplier during integration process
– On-site test support
Process via the Liquid Handling Software (LHS). – On-site installation support
HeLaS3 96-well Automation Nucleofector® Kit

VHCA-4001 96 reactions (smallest unit)
Neuro2a 96-well Automation Nucleofector® Kit

HeLa 96-well Automation Nucleofector® Kit

Please note: Automation Nucleofector® Kits are provided as customized

kits. Please contact our Scientific Support or your local account manager
for detailed information.
135
Automation Package
96-well Shuttle®
Example for Fully Automated Cell Transfection with Tecan and Lonza’s
New Combined Platform
Combining the Nucleofector® 96-well Shuttle® System and a Tecan® Freedom EVO®
liquid handling workstation allows fully automated, precise and efficient transfection
of difficult-to-transfect cell lines and primary cells. The integrated system is ideal
for large-scale studies that involve high throughput transfection stages such as
RNAi-based screening for target identification and validation, or screening of cDNA
libraries. The present platform will be used for siRNA experiments based on human T
lymphocytes and Jurkat cells using focused siRNA libraries.
Integration and Interaction
The 96-well Shuttle® System is positioned on the Freedom EVO® Worktable using
a special adapter plate. Nucleocuvette® Plates, which are first prepared using the
eight channel liquid handling arm or the multichannel option with stainless steel or
disposable tips, can be loaded on the 96-well Shuttle® System and retrieved after the
Nucleofection® Process using the robotic manipulator. The 96-well Shuttle® Software is
controlled by Tecan’s Freedom EVOware® Robotics Control Software using the interface
provided by Lonza.
Rapid, Precise and Reliable Liquid Handling
The freely configurable Freedom EVO® Workstation can accommodate a wide range
of modules, including the robotic manipulator arm to transfer plates to and from
the 96-well Shuttle® System. Integrating the 96-well Shuttle® System and a robotic
incubator with the Freedom EVO® Workstation completely substitutes all manual steps
for cell preparation and transfection, generating consistent reproducible results. The
platform can process a 96-well Nucleocuvette® Plate in just minutes, depending on
the protocol used.
The Freedom EVO® Workstation is available in different sizes and with a variety of
options, including:
■ Liquid handling arm with fixed or disposable (filter) tips
■ Multichannel pipettors for plating cells or direct transfer of compounds
from plate to plate
■ Robotic CO2 incubator for automated cell storage, delivery and incubation
■ Option for harvesting of adherent cells from robotic-friendly cell culture flasks
■ Cooled or heated carriers for media, nucleic acids and Nucleofector® Solution
■ Integrated thermocyclers for PCR applications
136
Automation Package
96-well Shuttle®
Process Control
Using the Lonza interface included in the 96-well Shuttle® Automation Package the
entire Nucleofection® Process is controlled by the Freedom EVOware® Software, which
allows for the implementation of individual and specific plate preparation protocols.
Nucleofection® conditions for the 96-well Shuttle® System are defined in the Lonza
software and the resulting parameter files are uploaded from Freedom EVOware®
Software and executed on the 96-well Shuttle® System.
All necessary steps for transfection can be automated, including:

■ Cell harvesting
■ Cell counting, diluting to the desired density and plating
■ Overnight incubation and cell washing
■ DNA/RNA normalization
■ Preparation and incubation of reagent mixes
■ Resuspension of cells and substrates in Nucleofector® Solution
prior to Nucleofection® Process
■ Nucleofection® Process
■ Incubation of transfected cells prior to analysis
■ Analysis of transfection results
137
List of Nucleofector® Kits for Primary Cells
Nucleofector® Kits
NF: Nucleofector® Kit available for this particular cell type

96: 96-well Shuttle® Kit available for this particular cell type
Cell Type Kit Transfection Viability

Efficiency
Blood Cells
B cell, human NF 36% 84 – 92%
B cell, human 96 28% 70%
B cell, mouse, stimulated NF 49 – 59% 27 – 47%
B cell, mouse, stimulated 96 55 – 56% 41 – 87%
CD34+ cell, human NF 71%
Dendritic cell, human NF 49% 71%
Dendritic cell, mouse, immature, BALB/c NF 58% 62%
Dendritic cell, mouse, immature, C57BL/6 NF 54% 52%
Dendritic cell, mouse, mature, BALB/c NF 49% 78%
Dendritic cell, mouse, mature, C57BL/6 NF 37% 63%
Dendritic cell, mouse, immature, BALB/c 96 27 – 43% 37 – 49%
Dendritic cell, mouse, immature, C57BL/6 96 23 – 34% 41 – 58%
Dendritic cell, mouse, mature, BALB/c 96 32% 85%
Dendritic cell, mouse, mature, C57BL/6 96 29% 88%
Macrophage, human NF 55 – 59% 87 – 88%
Macrophage, human 96 42% 60%
Macrophage, mouse, BALB/c NF 34 – 45% 84 – 92%
Macrophage, mouse, C57BL/6 NF 24 – 47% 80 – 88%
Monocyte, human NF 55 – 56% 62 – 81%
Monocyte, human 96 64% 77%
Natural killer (NK) cell, human NF 16% 50 – 60%
T cell, human, stimulated NF 36 – 47%
T cell, human, unstimulated NF 66%
T cell, human, stimulated 96 70% 59%
T cell, human, unstimulated 96 68 – 80% 53 – 79%
T cell, mouse, BALB/c NF 32 – 44% 18 – 55%
T cell, mouse, C57BL/6 NF 20 – 28% 17 – 45%
T cell, mouse, BALB/c 96 45% 32%
T cell, mouse, C57BL/6 96 43% 23%
Dermal Cells
Fibroblast, dermal (NHDF), human, adult NF 42 – 69% 74 – 77%
Fibroblast, dermal (NHDF), human, neonatal NF 82 – 90%
Fibroblast, dermal (NHDF), human, neonatal 96 86 – 98% 86 – 91%
Keratinocyte, human, adult NF 36 – 51% 40 – 60%
Keratinocyte, human, neonatal NF 39 – 53% 50 – 60%
Melanocyte, (NHEM-neo), human, neonatal NF 70% 55 – 60%
Endothelial Cells
Endothelial cell, human, coronary artery (HCAEC) NF 57% 42%
Endothelial cell, human, lung (HMVEC-L) NF 52% 52%
Endothelial cell, human, umbilical vein (HUVEC) NF 90% 74%
Endothelial cell, human, umbilical vein (HUVEC) 96 74% 66%
138
List of Nucleofector® Kits for Primary Cells
Continued
Nucleofector® Kits
Cell Type Kit Transfection Viability
Efficiency
Epithelial Cells
Epithelial cell, human, bronchial (NHBE) NF 52% 51%
Epithelial cell, human, bronchial (NHBE) 96 54% 53%
Epithelial cell, human, mammary (HMEC) NF 56 – 73% 66 – 98%
Epithelial cell, human, mammary (HMEC) 96 51% 66%
Epithelial cell, human, prostate (PrEC) NF 43% 64%
Epithelial cell, human, prostate (PrEC) 96 67% 48%
Hepatocytes
Hepatocyte, human 96 54% 59 – 69%
Hepatocyte, mouse NF 54% 80%
Hepatocyte, rat NF 52% 78%
Neural Cells
Astrocyte, mouse NF 60% 60 – 70%
Astrocyte, rat NF 59-67% 70 – 80%
Neuron, chicken, hippocampal NF 43%
Neuron, chicken, dorsal root ganglion NF 30%
Neuron, mouse, hippocampal NF 58%
Neuron, mouse, dorsal root ganglion NF 30 – 60% 25 – 65%
Neuron, rat, hippocampal/cortical NF 54 – 64% 47 – 52%
Neuron, rat, hippocampal/cortical 96 60%
Neuron, rat, dorsal root ganglion NF 41%
Oligodendrocyte, rat NF 44% 60%
Smooth Muscle Cells

Smooth muscle cell, human, aortic NF 68% 90 – 96%
Stem Cells
CD34+ cell, human NF 71%
Embryonic stem cell (H9), human 96 64% 98%
Embryonic stem cell, human NF 20 – 78% 50 – 96%
Embryonic stem cell, mouse NF 80%
Embryonic stem cell, mouse 96 77 – 90% 68 – 81%
Mesenchymal stem cell (MSC), human NF 30 – 47% 78 – 86%
Mesenchymal stem cell (MSC), human 96 67%
NOTE: Nucleofector® Kits contain a
Neural stem cell (NSC), mouse NF 40 – 60% proprietary nucleic acid coding for
a proprietary copepod fluorescent
Neural stem cell (NSC), rat NF 42 – 46%
protein intended to be used as positive
control with this Lonza product only.
Any use of proprietary nucleic acid
Other Cells or fluorescent protein other than
Cardiomyocyte, rat, neonatal NF 35% 50 – 60% as positive control with this Lonza
product is strictly prohibited. USE IN
Chondrocyte, human NF 65% 60 – 70% ANY OTHER APPLICATION REQUIRES
Chondrocyte, human 96 74% 84% LICENSE FROM EVROGEN. To obtain
such a license, please contact Evrogen
Embryonic fibroblast (MEF), mouse NF 43% 60 – 80% at license@evrogen.com.
139
Human B Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Efficient transfection of human B cells is a pre-requisite Ordering Information

to enable research in fields such as chemokine/antigen
Cat. No. Size Price
response or B cell activation. Now cited in a growing
number of publications, the Human B Cell Nucleofector® Human B Cell Nucleofector® Kit
Kit has been used for DNA, RNA and siRNA transfection.
VPA-1001 25 reactions
The versatility of the Nucleofector® Technology is ideal for
co-transfection of siRNA with a DNA plasmid as reporter
400
% CAT activity
gene, a setup required in rescue experiments or as 350
transfection control. 300
– For unstimulated and stimulated B cells 250

200
– Up to 36% transfection efficiency 150
– One protocol for DNA, siRNA, RNA 100
– Suitable for transfection of CLL cells derived from 50

0 CR2 promoter CR2 promoter CR2 promoter
patient material + p50 + p65
WT Control
Promoter studies in primary B cells prove that NF-κB regulates the activity
of the human CR2 promoter. Primary human B cells were transiently
co-transfected with a CAT reporter plasmid driven by wild-type (WT) CR2
promoter, and plasmids encoding NF-κB subunit p50 or p65 or a control
plasmid. Cells were assayed for CAT activity 15 hours post Nucleofection®.
Values represent percentage of CAT activity, considering the activity of the
empty vector control 0% and the activity of the wild-type CR2 promoter 100%.
The results demonstrate that both NF-κB subunits clearly induce CAT activity.
(Data reproduced from Tolnay et al. (2002) J Immunology, with permission
from the Journal of Immunology.)
Human B Cell 96-well Nucleofector® Kit
High throughput Nucleofection® of human B cells becomes Ordering Information

feasible with the Human B Cell 96-well Nucleofector® Kit.
Cat. No. Size Price
The unique Nucleocuvette® Plate allows working with cell
numbers of 1 x 106 cells per well and lower. Moreover, its Human B Cell 96-well Nucleofector® Kits
modular design offers highest flexibility concerning the
VHPA-1001 96 reactions
number of transfections performed per experiment.
– Low cell numbers
– Low plate variation Customized kits for more reactions are available upon request.
– Excellent performance with up to 28% efficiency
70
and 70% viability Buffy 1
% Transfection efficiency
Buffy 2
60
– Suitable for siRNA, shRNA, DNA plasmids, etc. 50
40
30
20
10
Clonetics®
C // C C
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 66 68 70 72 74 76 78 80 82 84 86 88 90 92 94 96
Well C== Control

Well-to-well uniformity of reporter gene expression of human B cells
transfected by 96-well Nucleofection®. Freshly isolated human B cells
were transfected by Nucleofection® with the pmaxGFP® Vector using the
Human B Cell 96-well Nucleofector® Kit. 24 hours post Nucleofection®,
cells were analyzed on a BD FACSCalibur™ Flow Cytometer with HTS option.
Wells without GFP expression are negative controls of cells in 96-well
Nucleofector® Solution and plasmid DNA, but without Nucleofection®.
140
Mouse B Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Mouse B cells are a frequently used model system in Ordering Information
various research fields, such as tumor immunology,
Cat. No. Size Price
signal transduction and B cell development. However,
transfection efficiencies reached by non-viral methods Mouse B Cell Nucleofector® Kit
like lipofection or electroporation were far below scientists'
expectations. The new Mouse B Cell Nucleofector® Kit
will bridge this methodological gap, therefore enabling
scientific approaches that are otherwise impossible with
today’s technology.
– Up to 59% efficiency and 47% viability
– Expression of cell typical marker proteins not effected A B
– One protocol for DNA, siRNA and RNA transfection
Nucleofection® of mouse B cells. Primary mouse B cells were transfected

by Nucleofection® using the Mouse B Cell Nucleofector® Kit and a plasmid
encoding maxGFP® Reporter Protein. Cells were then stimulated with LPS.
48 hours post Nucleofection® cells were analyzed by light (A) and
fluorescence microscopy (B).
Mouse B Cell 96-well Nucleofector® Kit
Mouse B cells play a central role in the humoral immune Ordering Information
response, thus understanding their functionality for
Cat. No. Size Price
example by loss and gain of function studies is of vital
interest for research groups in academia and industry. Mouse B Cell 96-well Nucleofector® Kit
The Mouse B Cell 96-well Nucleofector® Kit for stimulated
B cells allows high throughput transfection with a wide
variety of substrates enabling scientists to perform these
studies with much less time and labor input.
– Allowing best performance on various donor strains
– Up to 55% transfection efficiency
– Cells maintain their functionality A B
Example showing typical transfection results of stimulated mouse B

cells. Prior to Nucleofection®, mouse B cells were stimulated with LPS for
24 hours. Stimulated cells were transfected with program 96-DI-100 using
0.4 µg pmaxGFP® Vector. 24 hours post-transfection, GFP expression was
analyzed by light (A) and fluorescence microscopy (B).
141
Human CD34+ Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
In their role as hematopoietic progenitors, CD34+ cells Ordering Information

are of great interest to immunologists. They have been
Cat. No. Size Price
shown to differentiate into other non-hematopoietic cells,
such as endothelial precursors. So far, transfection of Human CD34+ Cell Nucleofector® Kit
CD34+ progenitor cells has only been possible with viral
methods or by electroporating stimulated cells. Using
Nucleofection®, unstimulated CD34+ cells can now be
transfected non-virally. For Nucleofection®, CD34+ cells
can be derived from cord blood or leukapheresis material. Peripheral Blood Stem Cells Bone Marrow Stem Cells
Both fresh or cryopreserved material can be used. 50 50
% Positive cells
% Positive cells
– Up to 70% transfection efficiency 40 40
– No influence on hematopoietic cell differentiation 30 30
– Cited for DNA and siRNA transfection 20 20
– For unstimulated primary CD34+ cells 10 10

0 4h 36 h 84 h 120 h 200 h 0 4h 36 h 84 h 120 h 200 h
Time Time
Long-term transgene expression after Nucleofection® in blood and bone

marrow derived CD34+ cells. Kinetics of deltaLNGFR (Low Affinity Nerve
Growth Factor Receptor) expression were determined by flow cytometric
analysis. 39 ± 5.9% of peripheral blood stem cells showed deltaLNGFR
staining 4 hours after transfection with a continuous decrease (n = 3, 3
patients). Bone marrow stem cells showed maximal deltaLNGFR expression
with 26 ± 9.7% 84 hours after transfection, which then decreased in the
proliferating culture (n = 3, single patient).
(Data courtesy of Greiner et al., University Hospital of Ulm, Ulm, Germany.)
Human Dendritic Cell Nucleofector® Kit
A rapidly growing interest in dendritic cells (DCs) as both Ordering Information

research and therapeutic tools reflects an increased Cat. No. Size Price
understanding and appreciation of their role at the heart
of the immune system and the broad range of functions Human Dendritic Cell Nucleofector® Kit
they perform. Human dendritic cells can be transfected
using the same Nucleofection® conditions with different
substrates such as RNA, DNA or siRNA.
– Transfection efficiencies up to 50%
– For immature and mature monocyte-derived
120 120
% Firefly luciferase expression
% Viability
dendritic cells 110 110

– For short term expression of up to 48 hours 100 100
90 90
80 80
70 70
Nucleofection® of human DCs with siRNA. Co-Nucleofection® of cells
60 60
with two plasmids and siRNA: a CMV-promoter driven firefly luciferase
construct (pCMV-Luc), a TK-promoter driven Renilla luciferase construct 50 50
(pRL-TK) as internal control reporter for normalization, and siRNA against 40 40
firefly luciferase (siRNA luc) or scrambled control siRNA (siRNA scr). 30 30
24 hours post Nucleofection®, an assay was performed measuring firefly
and Renilla luciferase expression in a consecutive fashion. Viability was 20 20
determined with an ATP-content assay. 10 10
(Data reproduced from Stallwood et al. (2006) J Immunol 177(2), 885-895 0 100 100 500 500 0 100 100 500 500
with permission of the authors.) no scr luc scr luc no scr luc scr luc
(Normalized to Renilla luciferase
as internal control reporter)
142
Mouse Dendritic Cell Nucleofector® Kit
Nucleofector® Kits
Neurons
Lonza’s R&D team has developed a Nucleofector® Kit for Ordering Information
the transfection of immature and mature mouse dendritic
Cat. No. Size Price
cells. For the first time, it is possible to investigate the
role and function of dendritic cells in the murine model Mouse Dendritic Cell Nucleofector® Kit
system using a non-viral transfection approach. Gene
VPA-1011 (Immature) 25 reactions
over-expression studies or RNAi mediated gene silencing
VPA-1012 (Mature) 25 reactions
can then easily be performed in these cells that play such
a pivotal role within immune defense.
A B
110 Control 110 Efficiency
% Functionality
Sample Viability
100 100
Functionality post Nucleofection®. The graph displays functionality of 90 90
immature mouse DCs (isolated from mice strain Balb/C) post Nucleofection® 80 80
(Sample) on the standard Nucleofector® Device. 2 hours post Nucleofection®, 70 70
cells were stimulated by LPS. 22 hours later, functionality was analyzed by
IL-6 specific ELISA and is given in percent compared to non-transfected 60 60
control. (A) 50 50
40 40
Transfection Performance. Mouse DC (Balb/C) were transfected according
30 30
to the appropriate optimized protocol for Nucleofection® using pmaxGFP®
Vector. Cells were analyzed 24 hours post Nucleofection® by flow cytometry 20 20
for maxGFP® Reporter Protein expression and viability. Cell viability is given 10 10
in percent compared to non-transfected control. (B) 0 0
Mouse Dendritic Cell 96-well Nucleofector® Kit
Mouse dendritic cells play a central role in the mammalian Ordering Information
immune system. Understanding dendritic cell functionali- Cat. No. Size Price
ty is of vital interest for both research groups and industry.
The two Mouse Dendritic Cell 96-well Nucleofector® Kits Mouse Dendritic Cell 96-well Nucleofector® Kits
allow high throughput transfection with a wide variety of
VHPA-1011 (Immature) 96 reactions
substrates, enabling scientists to perform such studies
VHPA-1012 (Mature) 96 reactions
more efficiently than ever before. Reproducible high
VHPA-2011 (Immature) 960 reactions
performance is guaranteed for both mature and immature
VHPA-2012 (Mature) 960 reactions
mouse dendritic cells using kits dedicated for either
mature or immature cells.
– High cell viability (up to 80%) 100
– Low well-to-well variation

90
80
70
Clonetics®
60
50
Well-to-well uniformity of reporter gene expression of mouse dendritic
40
cells transfected by 96-well Nucleofection®. Mouse dendritic cells were
transfected by Nucleofection® with the pmaxGFP® Vector using the Mouse 30
Dendritic Cell 96-well Nucleofector® Kit. 24 hours post Nucleofection®, 20
cells were analyzed on a BD FACSCalibur™ Flow Cytometer with HTS option. 10 C C C C // C C C C
Wells without GFP expression are negative controls of cells in Nucleofector®
Solution and plasmid DNA, but without Nucleofection®. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C== Control
143
Human Macrophage Nucleofector® Kit
Nucleofector® Kits
Blood Cells
With their multifunctional roles in antigen processing Ordering Information

and presentation, phagocytosis and cytokine secretion,
Cat. No. Size Price
macrophages are a prominent effector in cellular immunity.
The efficient transfection of primary macrophages (derived Human Macrophage Nucleofector® Kit
from peripheral blood monocytes) with Nucleofector®
Technology now opens new options for immunologists
and cancer researchers.
– Up to 60% transfection efficiency A B
– For transfection of resting macrophages
– Cited for DNA and siRNA transfection
– Maintenance of functionality (e.g., activation)
C D
Nucleofection® of human macrophages with pmaxGFP® Vector. Primary

human macrophages were transfected by Nucleofection® using the Human
Macrophage Nucleofector® Kit with a plasmid encoding maxGFP® Reporter
Protein. 24 hours post Nucleofection®, cells were analyzed by light (A, C) and
fluorescence microscopy (B, D). A and B show cells at 10 x magnification.
At 40 x magnification (C, D), transfected macrophages reveal cytoplasmic
extrusions important for phagocytic function of macrophages.
Human Macrophage 96-well Nucleofector® Kit
Human macrophages play a key role in cell-mediated Ordering Information

immunity. Hence, studying their functionality and role in
Cat. No. Size Price
health and disease is of great interest for pharmacologists,
immunologists and cancer research scientists. Lonza’s Human Macrophage 96-well Nucleofector® Kit
R&D team developed a Human Macrophage 96-well
Nucleofector® Kit enabling scientists to conduct high
throughput transfection in these cells. RNAi library
screenings and other high throughput screening 1.7
A405
approaches should help to elucidate the role of these key 1.6
mediators within the immunological system and may thus 1.5

1.4
become an essential tool to accelerate drug discovery 1.3
processes. 1.2
1.1
1.0
0.9
0.8
0.7
0.6
Well-to-well uniformity of SEAP reporter gene expression after 96-well 0.5
Nucleofection®. 1 x 105 cells were transfected by Nucleofection® with
0.4
0.4 µg of an expression vector encoding the secreted alkaline phosphatase
(SEAP) using the Human Macrophage 96-well Nucleofector® Kit. 0.3
24 hours post Nucleofection®, SEAP enzyme activity was measured (n=36, 0.2
SD = ± 14% from mean). Wells without SEAP enzyme activity are negative 0.1 C C // C
controls of cells in 96-well Nucleofector® Solution and plasmid DNA but 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
without Nucleofection®. Well C == Control
144
Mouse Macrophage Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Macrophages are frequently used in studies on basic Ordering Information
immunology and cancer research. With the Mouse
Cat. No. Size Price
Macrophage Nucleofector® Kit, you can now address
areas such as gene regulation, signaling pathways or Mouse Macrophage Nucleofector® Kit
identification of genes responsible for differentiation in
the murine in vitro model system.
– Evaluated for macrophages from C57BL/6 and
BALB/c strains
– For transfection of resting bone-marrow derived
macrophages
– Maintenance of functionality (e.g., activation)
A B
C D
Nucleofection® of mouse macrophages with pmaxGFP® Vector. Primary

mouse macrophages were transfected by Nucleofection® using the Mouse
Macrophage Nucleofector® Kit with a plasmid encoding maxGFP® Reporter
Protein. 24 hours post Nucleofection®, cells were analyzed by light (A, C) and
fluorescence microscopy (B, D). A and B show cells at 10 x magnification.
At 40 x magnification (C, D), transfected macrophages reveal cytoplasmic
extrusions important for phagocytic function.
145
Human Monocyte Nucleofector® Kits
Nucleofector® Kits
Blood Cells
The efficient transfection of primary human monocytes Ordering Information

allows research in areas such as cellular immunology, Cat. No. Size Price
signaling, differentiation, and activation to be addressed.
The Human Monocyte Nucleofector® Kit contains 100 ml Human Monocyte Nucleofector® Kit
of a specialized recovery medium that helps improve
post-transfection survival of monocytes transfected by
Nucleofection®. Human Monocyte Nucleofector® Medium
VZB-1003 1 x 500 ml
– Viability of 70% after 24 hours
VZB-4003 4 x 500 ml
– Complete solution – optimized recovery
medium included
– Cited for DNA and siRNA transfections
A B
240 14 No stimulus 9-HODE

Counts
fluorescence U/ml x 10-6

Adhesion (endpoint
200 12
96.37%
10
160
8
120
6
80
4
40 2
0 10 101 102 103 104 0 – Control PPARg #1
FL1-H sRNAi
C D
50 None Control PPARg #1 25 None Control PPARg #1

% CX3CR1+ cells
% CX3CR1+ cells
45
40 20
35
30 15
25
20 10
15
10 5
5
0 – 9-HODE 0 – 9-HODE
Lipid Lipid
Nucleofection® of human monocytes with Stealth™ RNAi (sRNAi).

(A) Efficiency of transfection was determined with 100 nM fluorescein-
labeled dsRNA oligomer (same length, charge and configuration as
the sRNAi) monitored 24 hours later by flow cytometry. Blue curve
shows autofluorescence. (B and C) Knockdown with Stealth™ RNAi
[Invitrogen]. Oxidized linoleic acid metabolites (like 9-HODE, 9-hydroxy-
10E, 12Z-octadecadienoic acid ester), components of oxidized LDL found
in large amounts in atherosclerotic plaque, are able to specifically induce
differentiation of human monocytes to macrophages accompanied by
a switch of chemokine receptor expression (CCR2-off and CX3CR1-on).
CX3CR1 then mediates macrophage adhesion to coronary artery smooth
muscle cells (CASMCs). The effects of the lipids on receptor expression are
mediated by the nuclear receptor peroxisome proliferator-activated receptor
(PPAR) gamma. Down regulation of PPARgamma with sRNAi (200 nM,
[Invitrogen]) dramatically reduced receptor switch (B) and consequently
macrophage adhesion to CASMCs in an adhesion assay (C). (Data extracted
from Barlic et al. (2006) Circulation 114(8), 807-19 with permission from
the authors.)
146
Human Monocyte 96-well Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Human monocytes are involved in the pathogenesis Ordering Information
of atherosclerosis. They play an important role in Cat. No. Size Price
immune defense, inflammation and tissue remodeling.
Understanding these processesis a key issue for drug Human Monocyte 96-well Nucleofector® Kits
development. The Human Monocyte 96-well Nucleofector®
Kit is tailored to high throughput application needs in
industry and academia.
– First high throughput transfection technology for Human Monocyte Nucleofector® Medium
human monocytes
VZB-1003 1 x 500 ml
– Excellent well-to-well reproducibility
VZB-4003 4 x 500ml
– 75% cell viability
– 80% fewer cells required compared to standard
100
90
80
70
60
50
40
30
20
10 C C // C C C C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C==Control
Well-to-well uniformity of reporter gene expression of enriched human

monocytes transfected by 96-well Nucleofection®. Freshly enriched
human monocytes were transfected by Nucleofection® with pmaxGFP®
Vector using the Human Monocyte 96-well Nucleofector® Kit. 24 hours post
Nucleofection®, cells were analyzed on a BD FACSCalibur™ Flow Cytometer
with HTS option. Wells without GFP expression are negative controls of
cells in 96-well Nucleofector® Solution and plasmid DNA, but without
Nucleofection®.
147
Human Natural Killer Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Cancer and immunology research was frequently Ordering Information

hampered due to the inability to transfect primary natural
Cat. No. Size Price
killer cells non-virally. With the Human Natural Killer
(NK) Cell Nucleofector® Kit, a major breakthrough was Human Natural Killer Cell Nucleofector® Kit
accomplished, enabling primary cells to be transfected
instead of cell lines. This kit has overcome the major
obstacles of NK cell transfection and, with efficiencies
of up to 20%, can provide a major advancement to your
research.
– First non-viral transfection technology for
primary NK cells
– Viability post Nucleofection® 50 - 60%
A NK cells -DNA B NK cells +DNA

SSC
SSC
0.5% 16.9%
eGFP eGFP
Nucleofection® of primary human NK cells. Polyclonal human NK cells

generated from PBMC co-cultured with the feeder cell line RPMI 8866 for
9 days were transfected by Nucleofection® with a plasmid encoding eGFP
protein. Cells were analyzed by flow cytometry 24 hours post Nucleofection®.
eGFP expression in natural killer cells is shown after Nucleofection® without
(A) and with plasmid DNA (B).
(Courtesy of J. Sundbäck and K. Kärre, Karolinska Institute, Microbiology
and Tumor Biology Center, Stockholm, Sweden.)
148
Human T Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
The Nucleofection® of unstimulated and stimulated human Ordering Information
T cells has become a significant tool in immunology
Cat. No. Size Price
research. For optimal transfection results, Lonza offers
specially developed optimized protocols for stimulated Human T Cell Nucleofector® Kit
and unstimulated T cells. A huge number of publications
in which T cells have been transfected with DNA plasmids,
shRNA vectors and siRNA prove that T cells transfected
by Nucleofection® maintain functionality. RNAi-mediated A B C
gene silencing could thus be shown to address a variety
of scientific questions in primary T cells.
side scatter
– For unstimulated and stimulated T cells
CD3-APC
PJ
forward scatter FL1 FL2
D + DNA E + DNA
0.14% 60.64%
Nucleofection® of human T cells with pmaxGFP® Vector. PBMC were freshly

isolated from buffy coat and transfected by Nucleofection® with pmaxGFP®
Vector. 24 hours post Nucleofection®, cells were analyzed by flow cytometry.
Lymphocytes were gated according to forward/side scatter (A). T cells were
CD3-APC
CD3-APC
stained with antibody directed against CD3. Dead cells were excluded by
propidium iodide staining and gating (B/C). maxGFP® Reporter Protein
expression of T cells is shown after Nucleofection® without (D) and with
plasmid DNA (E). FL1 FL1
Human T Cell 96-well Nucleofector® Kit
As human T cells play such a central role in cell-mediated Ordering Information

immunity, they are of major interest for immunological
Cat. No. Size Price
studies and, consequently, for drug discovery studies. The
Human T Cell 96-well Nucleofector® Kit enables efficient Human T Cell 96-well Nucleofector® Kits
non-viral high throughput transfection of unstimulated,
as well as stimulated, human T cells for the first time. RNAi VHPA-1002 96 reactions
screenings performed directly in primary T cells instead VHPA-2002 960 reactions

of T cell lines may thus become a new useful approach to
address questions within basic as well as pharmaceutical
research.
– High transfection performance – up to 68% efficiency
at 87% viability 100 Buffy 1
Buffy 2
– Transfected cells preserve their biochemical 90
functionality 80
70
60
50
Well-to-well uniformity of reporter gene expression of human T cells
40
transfected with the Nucleofector® 96-well Shuttle® System. Human T
cells were transfected by Nucleofection® with pmaxGFP® Vector using the 30
Human T Cell 96-well Nucleofector® Kit. 24 hours post Nucleofection®, cells 20
were analyzed on a BD FACSCalibur™ Flow Cytometer with HTS option. Wells 10 C C C C
without GFP expression are negative controls of cells in Nucleofector® //
Solution and plasmid DNA, but without Nucleofection®. 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C== Control
149
Mouse T Cell Nucleofector® Kit
Nucleofector® Kits
Blood Cells
Transfection of primary mouse T cells represents a major Ordering Information

breakthrough in addressing research areas such as T cell
Cat. No. Size Price
function, activation, regulation, and signaling in this model
in vitro system. The combination of specific Nucleofector® Mouse T Cell Nucleofector® Kit
Solution, electrical parameters and optimized recovery
medium is essential for transfection efficiency and
post-transfection survival of mouse T cells transfected by
Nucleofection®.
– Viability after transfection up to 55%
– Evaluated for C57BL/6 and BALB/c strains 120 48h
% CD25 expressing CD4+ cells

72h
– Maintenance of functionality (e.g., stimulation) 100
– Complete solution – optimized recovery 80

60
medium included
40
20
0 –Nucleofection® /–DNA +Nucleofection® /–DNA +Nucleofection® /+DNA
Transfected and non-transfected mouse T cells can be stimulated

equally well. Primary C57BL/6 mouse T cells were transfected using
Nucleofection® with pmaxGFP® Vector. 3 hours post Nucleofection®, cells
were stimulated with plate bound anti-CD3 and anti-CD28. 48 and 72 hours
post Nucleofection®, CD4+ cells were analyzed for CD25 surface expression.
Figure shows proportion of CD25-expressing cells among living CD4+ T cells.
(%CD25 expression in unstimulated samples ranged from 10-20%.)
Mouse T Cell 96-well Nucleofector® Kit
Mouse T cells are an excellent in vitro model system Ordering Information

to elucidate the role of T cells in the immune system.
Cat. No. Size Price
Using the Mouse T Cell 96-well Nucleofector® Kit over-
expression studies or RNAi mediated gene silencing can Mouse T Cell 96-well Nucleofector® Kits
now be studied in a high throughput framework in these
primary cells.
– Post-transfection cell viability up to 35% 90
– Optimized protocols for C57BL/6 and BALB/c strains 80
– 80% fewer cells required compared to standard 70

60
50
40
30
20
10 C C C C // C C C C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C== Control
Well-to-well uniformity of reporter gene expression of mouse T cells trans-

fected by 96-well Nucleofection®. Freshly isolated mouse T cells (BALB/c)
were transfected by Nucleofection® with 0.5 µg pmaxGFP® Vector using
the Mouse T Cell 96-well Nucleofector® Kit. 24 hours post Nucleofection®,
cells were analyzed on a BD FACSCalibur™ Flow Cytometer with HTS option.
Wells without GFP expression are negative controls of cells in 96-well
Nucleofector® Solution and plasmid DNA, but without Nucleofection®.
150
Human Dermal Fibroblast (NHDF) Nucleofector® Kit
Nucleofector® Kits
Dermal Cells
Human dermal fibroblasts are used to study the Ordering Information
biochemical basis of various normal and disease
Cat. No. Size Price
processes. The efficient and reproducible transfection
of NHDFs with the Nucleofector® Technology has Human Dermal Fibroblast (NHDF) Nucleofector® Kit
provided a major advancement in studying diseases
VPD-1001 25 reactions
related to fibroblast cells such as fibrosarcoma, fibrosis,
scleroderma, and xeroderma pigmentosum.
A B
Related Products
Nucleofection® of adult human dermal fibroblasts with eGFP cDNA. Adult
NHDF were transfected by Nucleofection® using the Human Dermal NHDF-Ad - Human Adult Dermal Fibroblasts 38
Fibroblast Nucleofector® Kit, a plasmid encoding eGFP and program
U-23/U-023. 24 hours post Nucleofection®, cells were analyzed by light (A) NHDF-Neo - Neonatal Human Dermal Fibroblasts 38
and fluorescence microscopy (B). FGM®-2 BulletKit® 38
0.1 µg maxGFP™
0.4 µg maxGFP™
Human
100
Dermal Fibroblast (NHDF) 96-well Nucleofector® Kit
90
80
70
60
50
40
Human dermal fibroblasts are commonly used to
30 Ordering Information
20
elucidate the molecular10
basis of various skin diseases.
C C // C Cat. No. Size Price
Pharmacological
2 4 6 8 10 12 14studies
16 18 20 22 24 to
0 26 28reveal
30 32 66 68the
70 72 74function of88 genes
76 78 80 82 84 86 90 92 94 96
Well C = Control
related to fibroblast based diseases, like fibrosarcoma NHDF 96-well Nucleofector® Kits
or fibrosis, involve high throughput knockdown using
VHPD-1001 96 reactions
siRNA. With the new Human Dermal Fibroblast 96-well
VHPD-2001 960 reactions
Nucleofector® Kit, it is possible to perform Nucleofection®
of various substrates in 96-well format.
0.1 µg pmaxGFP® Vector 0.4 µg pmaxGFP® Vector
100
90
80
70
60
50
40
30
20
10 C C // C
0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 66 68 70 72 74 76 78 80 82 84 86 88 90 92 94 96
Well C = Control
Well-to-well uniformity of reporter gene expression of NHDF-Neo transfected Related Products

with the Nucleofector® 96-well Shuttle® System. NHDF-Neo cells were
transfected by Nucleofection® with the pmaxGFP® Vector using the NHDF
96-well Nucleofector® Kit. 24 hours post Nucleofection®, cells were analyzed NHDF-Ad - Human Adult Dermal Fibroblasts 38
on a BD FACSCalibur™ Flow Cytometer with HTS option. Wells without GFP NHDF-Neo - Neonatal Human Dermal Fibroblasts 38
expression are negative controls of cells in 96-well Nucleofector® Solution
and plasmid DNA but without Nucleofection®. FGM®-2 BulletKit® 38
151
Human Keratinocyte Nucleofector® Kit
Nucleofector® Kits
Dermal Cells
Transfection of primary keratinocytes is an essential Ordering Information

tool for studying various cellular functions such as gene
Cat. No. Size Price
expression, intracellular signaling and promoter studies.
Commonly used non-viral transfection methods usually Human Keratinocyte Nucleofector® Kit
display certain drawbacks such as low transfection
efficiency and the induction of terminal differentiation. VPD-1002 25 reactions
Nucleofection® offers a new highly effective non-viral

alternative that does not induce terminal differentiation in
transfected keratinocytes (Distler et al. 2005).
– For neonatal and adult keratinocytes
– Maintenance of functionality
(e.g., no terminal differentiation)
Day 1
Day 2
Day 5
Keratinocytes transfected by Nucleofection® survive and show eGFP

expression for more than one week. Neonatal keratinocytes were transfected
by Nucleofection® with eGFP cDNA and analyzed by light and fluorescence
microscopy after 1, 2 and 5 days.
152
NHEM-Neo (Normal Human Epidermal Melanocyte-Neo) Nucleofector® Kit
Nucleofector® Kits
Dermal Cells
Melanocytes, the pigment-producing cells of the epider- Ordering Information
mis, undergo malignant transformation in malignant
Cat. No. Size Price
melanoma. Melanoma cells can metastasize to almost
every major organ and tissue. Nucleofection® of neonatal NHEM-Neo Nucleofector® Kit
melanocytes is a major pre-requisite to elucidate this
transformation process.
– Reproducible non-viral transfection
– For DNA and siRNA transfection
A B
Nucleofection® of NHEM-Neo with eGFP cDNA. NHEM-Neo were transfected

by Nucleofection® using the NHEM-Neo Nucleofector® Kit and a plasmid
encoding enhanced green fluorescent protein, eGFP. 24 hours post
Nucleofection®, cells were analyzed by light (A) and fluorescence
microscopy (B).
153
Basic Nucleofector® Kit for Primary Mammalian Fibroblasts
Nucleofector® Kits
Dermal Cells
The Basic Nucleofector® Kit for Primary Mammalian Ordering Information

Fibroblasts is the ideal tool to transfect a multitude of
Cat. No. Size Price
fibroblasts from different mammalian species (e.g., mouse,
rat, human) and from various organs (e.g., lung, dermis). Basic Nucleofector® Kit for Primary Mammalian Fibroblasts
– Suitable for virtually any primary mammalian
VPI-1002 25 reactions
fibroblast cell type
– Testing of just five selected programs with one
Nucleofector® Solution
– Already tested for macaque dermal fibroblasts, bovine
fibroblasts, human colon myofibroblasts, mouse lung
fibroblasts, etc.
A siN-cad B rTGF-b1 2 days rTGF-b1 4 days
con 2+3 3+4

N-cadherin
con
relative intensity 1 1.03 0.05
cadherin-11
tubulin 2+3
SIN-CAD
3+4
Knockdown of N-cadherin by Nucleofection® of primary human colon

myofibroblasts with siRNA. (A) Western blot showing the knockdown of
N-cadherin upon Nucleofection® of myofibroblasts with combinations of
specific siRNAs derived from different regions of N-cadherin cDNA (siN-cad
2+3 or 3+4) or control siRNA (con). Cadherin-11 expression is unchanged.
Tubulin was used as loading control. (B) Inhibiting effect of N-cadherin
knockdown on TGF-b-stimulated invasion of myofibroblasts in a spheroid-
cell-collagen-invasion-assay after 2 and 4 days.
(Data reproduced from De Wever et al. (2004) J Cell Sci 117(Pt 20), 4691-703
with permission of the Company of Biologists and of the authors.)
Related Products
Fibroblast Cells 36 – 39
FGM®-2 BulletKit® 38
154
Basic 96-well Nucleofector® Kits for Primary Mammalian Fibroblasts
Nucleofector® Kits
Dermal Cells
Fibroblasts are commonly used to elucidate the molecular Ordering Information
basis of various fibroblast-based diseases like fibrosarco-
Cat. No. Size Price
ma or fibrosis. Furthermore, fibroblasts are utilized as
"feeder cells" in human embryonic stem cell research Basic 96-well Nucleofector® Starter Kit for
and for reprogramming into iPS (induced pluripotent Primary Mammalian Fibroblasts
stem) cells. The Basic 96-well Nucleofector® Starter
VHPI-1002 64 reactions
Kit and the Basic 96-well Nucleofector® Kits 1 and 2 for
Primary Mammalian Fibroblasts allow high throughput Basic 96-well Nucleofector® Kit 1 for
Nucleofection® with any type of mammalian fibroblasts. Primary Mammalian Fibroblasts
– Economical starter kit for fast determination
of ideal Nucleofection® conditions
– Two specialized basic kits suited to your
specific cell type Basic 96-well Nucleofector® Kit 2 for
Primary Mammalian Fibroblasts
A B
Nucleofection® of primary fibroblasts. NHDF-adult cells were transfected

with 0.4 µg pmaxGFP® Vector using the Basic 96-well Nucleofector® Kit for
Primary Mammalian Fibroblasts. 48 hours post Nucleofection®, cells were
analyzed by light (A) and fluorescence (B) microscopy.
Related Products
Fibroblast Cells 36 – 39
FGM®-2 BulletKit® 38
155
Human Coronary Artery Endothelial Cell (HCAEC) Nucleofector® Kit
Nucleofector® Kits
Endothelial Cells
The coronary endothelium is important for cardiac function Ordering Information

and coronary blood flow. The efficient gene transfer into
Cat. No. Size Price
coronary endothelial cells was previously limited to viral
systems. With Nucleofection® of coronary endothelial HCAEC Nucleofector® Kit
cells, you can now address various fields in cardiovascular
VPB-1001 25 reactions
research such as thrombosis, atherosclerosis and
hypertension.
A B
Example for the Nucleofection® of HCAEC. HCAEC were transfected by

Related Products
Nucleofection® with a plasmid encoding the fluorescent protein eGFP using
the HCAEC Nucleofector® Kit. 25 hours post Nucleofection®, cells were HCAEC - Coronary Artery Endothelial Cells 25
analyzed by light (A) and fluorescence microscopy (B). EGM®-2MV BulletKit® 33
Human Microvascular Endothelial Cell-Lung (HMVEC-L) Nucleofector® Kit
The vascular endothelium serves as a barrier between Ordering Information

blood and tissues. It participates in physiological
Cat. No. Size Price
processes and disorders, such as angiogenesis or
atherosclerosis. Human microvascular endothelial cells HMVEC-L Nucleofector® Kit
of the lung (HMVEC-L) are frequently used as a model
system for microvascular pathobiology. Nucleofector® VPB-1003 25 reactions
Technology enables efficient transfection of HMVEC-L

without the use of a viral system.
A B
Example for the Nucleofection® of HMVEC-L. HMVEC-L were transfected by Related Products
Nucleofection® using the HMVEC-L Nucleofector® Kit and a plasmid encoding
the enhanced green fluorescent protein eGFP. 25 hours post Nucleofection®, HMVEC-L - Lung Microvascular Endothelial Cells 26
cells were analyzed by light (A) and fluorescence microscopy (B). EGM®-2MV BulletKit® 33
156
Human Umbilical Vein Endothelial Cell (HUVEC) Nucleofector® Kit
Nucleofector® Kits
Endothelial Cells
HUVEC is an easy-to-isolate endothelial cell type, Ordering Information
commonly used in cancer and cardiovascular research to
Cat. No. Size Price
address areas such as angiogenesis and cardiovascular
diseases. Using the HUVEC Nucleofector® Kit, high HUVEC Nucleofector® Kit
transfection efficiencies and protein expression levels can
VPB-1002 25 reactions
be achieved. It is the ideal tool for protein over-expression
studies or RNAi experiments.
3.5
Chemiluminescence (x104 cpm)
3.0 SOD
- siRNA
2.0
NADH
1.0 + siRNA
0
10 20 30
Time (min)
Nucleofection® of HUVECs with siRNA. Knockdown of the NAD(P)H oxidase

Nox4 with siRNA shows that Nox4 is the major source for superoxide
production in the nucleus of HUVECs. 48 hours after Nucleofection® of
HUVECs with Nox4 siRNA, the nuclear fraction was prepared and superoxide Related Products
production was determined as superoxide dismutase (SOD)-inhibitable
chemiluminescence detected with a luminol-based test. The reaction was HUVEC - Human Umbilical Vein Endothelial Cells 33
started by the addition of NADH and stopped by addition of SOD.
(Data from Kuroda et al. (2005) Genes Cells 10(12), 1139-1151.) EGM®-2 BulletKit® 35
Human Umbilical Vein Endothelial Cell (HUVEC) 96-well Nucleofector® Kit
The 96-well Nucleofector® Kit for HUVEC cells allows non Ordering Information
viral, high throughput transfection of HUVEC with up to 96
Cat. No. Size Price
different substrates in parallel. It opens the possibility to
perform projects like siRNA screening within your drug HUVEC 96-well Nucleofector® Kits
discovery process.
– Excellent performance with up to 85% efficiency VHPB-1002 96 reactions
and 65% viability VHPB-2002 960 reactions
80
70
60
50
40
30
20
10 C C C C // C C C C
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C == Control
Well-to-well uniformity of reporter gene expression of 96-well transfected

primary HUVECs. HUVECs were transfected by Nucleofection® with pmaxGFP® Related Products
Vector using the HUVEC 96-well Nucleofector® Kit. 24 hours post Nucleofection®,
cells were analyzed on a BD FACSCalibur™ Flow Cytometer with HTS option. Wells HUVEC - Human Umbilical Vein Endothelial Cells 33
without GFP expression are negative controls of cells in 96-well Nucleofector®
Solution and plasmid DNA but without Nucleofection®. EGM®-2 BulletKit® 35
157
Basic Nucleofector® Kit for Primary Mammalian Endothelial Cells
Nucleofector® Kits
Endothelial Cells
The Basic Nucleofector® Kit for Primary Endothelial Cells Ordering Information
is the ideal tool to transfect a multitude of endothelial
Cat. No. Size Price
cells from different mammalian species (e.g., mouse, rat,
human) and from various organs (e.g., lung, brain, liver). Basic Nucleofector® Kit for
– Suitable for virtually any primary mammalian Primary Mammalian Endothelial Cells
endothelial cell type
Nucleofector® Solution
– Already tested for porcine capillary endothelial cells,
sheep uterine artery endothelial cells, etc.
A B
Example for Nucleofection® of primary porcine endothelial cells. Primary

porcine trabecular meshwork cells (derived from eye) were transfected
by Nucleofection® with the Basic Nucleofector® Kit for Primary Mammalian
Endothelial Cells, program T-23/T-023 and a plasmid encoding the green
fluorescent maxGFP® Reporter Protein. 24 hours post Nucleofection®, the
cells were analyzed by light (A) and fluorescence microscopy (B).
(Data courtesy of Dr. Ted Acott, Oregon Health & Science University, USA.)
Related Products
Endothelial Cells & Media 23 – 35
EGM®-2 BulletKit® 35
158
Normal Human Bronchial Epithelial Cell (NHBE) Nucleofector® Kit
Nucleofector® Kits
Epithelial Cells
Revealing a gene’s role in human bronchial cells may help Ordering Information
in understanding the formation and progression of human
Cat. No. Size Price
lung carcinoma. Transfection of primary human bronchial
epithelial cells isolated from a carcinoma is an important Normal Human Bronchial Epithelial Cell (NHBE)
method to study gene function. The NHBE Nucleofector® Nucleofector® Kit
Kit enables you to easily verify previous cell line results in
VPK-1001 25 reactions
the analogous primary cell type.
A B
Related Products
Example of Nucleofection® of NHBE. Normal human bronchial epithelial
cells were transfected with 2 µg pmaxGFP® Vector using the Normal Human
Bronchial Epithelial Cell Nucleofector® Kit. 24 hours post Nucleofection®, NHBE - Bronchial /Tracheal Epithelial Cells 19
cells were analyzed by light (A) or fluorescence (B) microscopy. BEGM® BulletKit® 19
Normal Human Bronchial Epithelial Cell (NHBE) 96-well Nucleofector® Kit
In the past, high throughput transfection of primary hu- Ordering Information

man bronchial epithelial cells has constituted a challenge.
Cat. No. Size Price
However, the use of cell lines instead of primary NHBE
limit the expressiveness of experimental data. The new Normal Human Bronchial Epithelial Cell (NHBE)
NHBE 96-well Nucleofector® Kit allows researchers to 96-well Nucleofector® Kits
study cancer related genes in NHBE cells directly, thus
VHPK-1001 96 reactions
overcoming all limitations coupled to the use of cell lines.
1.1
A405
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
C C C C C C C C
0.1
//
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C = Control
Well-to-well uniformity of SEAP reporter gene expression after 96-well

Nucleofection®. 7.5 x 104 cells were transfected by Nucleofection® with 0.4 µg
of an expression vector encoding the secreted alkaline phosphatase (SEAP) Related Products
using the NHBE 96-well Nucleofector® Kit. 24 hours post Nucleofection®,
SEAP enzyme activity was measured (n=60, SD = ± 11% from mean). NHBE - Bronchial /Tracheal Epithelial Cells 19
Wells without SEAP enzyme activity are negative controls of cells in
96-well Nucleofector® Solution and plasmid DNA but without Nucleofection®. BEGM® BulletKit® 19
159
Human Mammary Epithelial Cell (HMEC) Nucleofector® Kit
Nucleofector® Kits
Epithelial Cells
Breast cancer is one of the most widespread carcinomas Ordering Information

in humans. Transfection of cells derived from the original
Cat. No. Size Price
tumor, i.e., primary human mammary epithelial cells, is one
important tool to explore a gene’s role in the development Human Mammary Epithelial Cell (HMEC)
and progression of breast cancer. These cells can now be Nucleofector® Kit
efficiently transfected without the need of a viral system
using the HMEC Nucleofector® Kit. For the transfection of
mammary epithelial cells from other mammalian species,
we recommend using our Basic Nucleofector® Kit for
Primary Epithelial Cells.
– First efficient non-viral transfection method
for HMECs
– Up to 75% transfection efficiency after 24 hours
– More than 90% viability
– High reproducibility
DAPI α-tubulin Rit42 Merge
Lucif.-SiRNA
Rit42-siRNA
siRNA Nucleofection® in HMECs show that Rit42 inhibition leads to decreased

accumulation of acetylated α-tubulin and disrupts spindle fiber formation.
Cells were transfected by Nucleofection® with Rit42- or control (luciferase)
siRNA. 48 hours after transfection, cells were co-stained with DAPI (blue),
anti-α-tubulin monoclonal antibody (green), and anti-Rit42 polyclonal
antibodies (red). Merged images are shown in the last column.
(Courtesy of Kyung-tae Kim, Hematology and Oncology Division, Beth
Israel Deaconess Medical Center and Harvard Medical School, Boston,
Massachusetts, USA.)
Related Products
HMEC - Mammary Epithelial Cells 48
MEGM® BulletKit® 48
160
Human Mammary Epithelial Cell (HMEC) 96-well Nucleofector® Kit
Nucleofector® Kits
Epithelial Cells
HMECs are the ideal model system to elucidate the role of Ordering Information
breast cancer related genes in a primary tissue context.
Cat. No. Size Price
However, time and labor efficient high throughput
studies were hard to perform in these cells due to Human Mammary Epithelial Cell (HMEC)
the lack of appropriate and easy-to-use transfection 96-well Nucleofector® Kits
systems. The Human Mammary Epithelial Cell (HMEC)
96-well Nucleofector® Kit now renders RNAi mediated
gene silencing, as well as over-expression approaches
possible.
– Up to 51% efficiency
– Around 66% viability
– Low well-to-well variation
– No lot-to-lot variations
1.4
A405
1.3
1.2
1.1
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
C C CC C C C C
0.1
//
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C == Control

Nucleofection®. 1 x 105 cells were transfected by Nucleofection® with 1 µg of
an expression vector encoding the secreted alkaline phosphatase (SEAP)
using the HMEC 96-well Nucleofector® Kit. 24 hours post Nucleofection®,
SEAP enzyme activity was measured (n = 72, SD = ± 11.5% from mean).
Wells without SEAP enzyme activity are negative controls of cells in 96-well
Nucleofector® Solution and plasmid DNA but without Nucleofection®.
Related Products
HMEC - Mammary Epithelial Cells 48
MEGM® BulletKit® 48
161
Human Prostate Epithelial Cell (hPrEC) Nucleofector® Kit
Nucleofector® Kits
Epithelial Cells
Over-expression or down-regulation of genes using Ordering Information

transfection is a helpful tool for the identification and
Cat. No. Size Price
validation of target genes. However, the lack of an efficient
non-viral transfection method for primary human prostate Human Prostate Epithelial Cell (hPrEC) Nucleofector® Kit
epithelial cells often made this a laborious task. With the
hPrEC Nucleofector® Kit, primary human prostate epithelial
cells can now be efficiently transfected - fast and easy.
A B
Related Products
Example for Nucleofection® of human PrECs. Normal human prostate epithelial
cells were transfected with 2 µg pmaxGFP® Vector using the Human Prostate PrEC - Prostate Epithelial Cells 52
Epithelial Cell (hPrEC) Nucleofector® Kit. 24 hours post Nucleofection®, cells
were analyzed by light (A) or fluorescence microscopy (B). PrEGM™ BulletKit® 52
Human Prostate Epithelial Cell (hPrEC) 96-well Nucleofector® Kit
Normal human prostate epithelial cells are often used Ordering Information
to study potential causes of cancer by knock down or
Cat. No. Size Price
induction of cancer related genes. The new hPrEC 96-well
Nucleofector® Kit is the perfect tool for such projects. Human Prostate Epithelial Cell (hPrEC)
96-well Nucleofector® Kits
1.0 VHPK-2003 960 reactions
A405
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1 C C C C
//
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96
Well C == Control

Nucleofection®. 1 x 105 cells were transfected by Nucleofection® with 0.4 µg
of an expression vector encoding the secreted alkaline phosphatase (SEAP) Related Products
using the hPrEC 96-well Nucleofector® Kit. 24 hours post Nucleofection®,
SEAP enzyme activity was measured (n = 73, SD = ± 10% from mean). PrEC - Prostate Epithelial Cells 52
Wells without SEAP enzyme activity are negative controls of cells in 96-well
Nucleofector® Solution and plasmid DNA but without Nucleofection®. PrEGM™ BulletKit® 52
162
Basic Nucleofector® Kit for Primary Mammalian Epithelial Cells
Nucleofector® Kits
Epithelial Cells
The Basic Nucleofector® Kit for Primary Epithelial Cells is Ordering Information
the ideal tool to transfect a multitude of epithelial cells from
Cat. No. Size Price
different mammalian species (e.g., mouse, rat, human)
and from various organs (e.g., lung, liver, kidney). Basic Nucleofector® Kit for
Primary Mammalian Epithelial Cells
Example for Nucleofection® of primary human renal proximal tubular epithelial cells. Human renal proximal tubular epithelial
cells were transfected by Nucleofection® with the Basic Nucleofector® Kit for Primary Mammalian Epithelial Cells, program
U-17/U-017 and a plasmid encoding the green fluorescent protein, eGFP. 48 hours post Nucleofection®, cells were analyzed
by fluorescence microscopy.
(Data courtesy of Chang Xu, Robert L. Bacallao*, and Seth L. Alper. Department of Medicine, Beth Israel Deaconess Medical
Center and Harvard Medical School, Boston, Massachusetts, USA, *University of Indiana School of Medicine, Indianapolis,
USA.)
Basic 96-well Nucleofector® Kit for Primary Mammalian Epithelial Cells
With the new Basic 96-well Nucleofector® Kit for Primary Ordering Information
Mammalian Epithelial Cells, Nucleofection® of epithelial
Cat. No. Size Price
cells, is now a high throughput application. It can be used
for screening approaches with DNA as well as siRNA and Basic 96-well Nucleofector® Kits for
shRNA vectors. Primary Mammalian Epithelial Cells
Basic 96-well Nucleofector® Kit 1 for

Basic 96-well Nucleofector® Kit 2 for

The Approach
1. 2. 3. 1. Choose your primary epithelial cell type of

interest.
Program
Program Program Transfection
Transfection
Transfection 2. Test one 96-well Nucleofector® Solution in
efficiency
efficiency (%)(%) (%)
efficiency Viability
Viability (%) (%)
(%)
Viability combination with seven different 96-well
96-CM-102 Nucleofector® Programs. In addition, you
96-CM-102
96-CM-102
Program 3030Transfection
30 3030 30
efficiency (%) Viability (%) can also titrate different cell numbers.
96-DC-100
96-DC-100
96-DC-100 5050 50 4040 40
96-CM-102 30 30
96-EA-104 3. Identify the optimal 96-well Nucleofector®
96-EA-104 96-DC-100 7070 70
96-EA-104 50 606040 60
96-EL-110
96-EL-110 96-EA-104 7070 70 707060 Program (and additionally, optimal cell
96-EL-110 70 70 > >> >> > > >
96-EL-110 70 number) for best transfection performance.
96-CM-113
96-CM-113
96-CM-113 6060 60 606070 60 > > >
96-CM-113 60 60
96-DS-109
96-DS-109 96-DS-109 5050 50
96-DS-109 50 505050 50
Related Products
Airway Epithelial Cells & Media 18 – 20
Renal Cells & Media 54 – 57
163
Human Hepatocyte 96-well Nucleofector® Kit
Nucleofector® Kits
Hepatocytes
Human hepatocytes are frequently used to study Ordering Information

metabolic pathways and toxic effects of new therapeutic
Cat. No. Size Price
agents. However, due to their low proliferation ability
in-vitro, primary hepatocytes are difficult to transfect Human Hepatocyte 96-well Nucleofector® Kits
by classical, non-viral methods. Our new Nucleofector®
VHPL-1001 96 reactions
Kit solves this problem by offering excellent transfection
VHPL-1002 960 reactions
rates for DNA and siRNA.
– Up to 69% cell viability
– Cells remain functionality for up to 120 hours A B
Example showing typical Nucleofection® results of human hepatocytes.

Cryopreserved human hepatocytes were transfected with 1 µg pmaxGFP®
Vector using the Human Hepatocyte 96-well Nucleofector® Kit. 120 hours
post Nucleofection®, cells were analyzed by light (A) and fluorescence
microscopy (B).
Mouse Hepatocyte Nucleofector® Kit
Transfection of primary mouse hepatocytes represents Ordering Information

a major breakthrough in gastroenterology research.
Cat. No. Size Price
Efficiencies of up to 60% can be achieved using
Nucleofection®. The Nucleofector® Kit for freshly isolated Mouse Hepatocyte Nucleofector® Kit
mouse hepatocytes is suitable for both DNA and siRNA
VPL-1002 25 reactions
transfer, while functionality of cells is not affected.
A B
0.25
Albumin secretion [pg/cell/hour]
0.2
0.15
0.1
0.05
0 Untreated cells Cells transfected

by Nucleofection®
Nucleofection® of mouse hepatocytes. Primary mouse hepatocytes were Hepatocytes transfected by Nucleofection® maintain functionality. 24 hours
transfected with 4 µg pmaxGFP® Vector using the Mouse Hepatocyte post-plating, primary mouse hepatocytes transfected by Nucleofection®
Nucleofector® Kit. 24 hours post Nucleofection®, cells were analyzed by and non Nucleofection® Transfected Control Cultures were analyzed for
light (A) and fluorescence microscopy (B). albumin secretion (normalized to cell number) by ELISA.
164
Rat Hepatocyte Nucleofector® Kit
Nucleofector® Kits
Hepatocytes
Rat hepatocytes are involved in numerous metabolic Ordering Information
pathways and have important functions that include
Cat. No. Size Price
detoxification and synthesis of proteins, lipids and
bile acids. With the first efficient non-viral transfection Rat Hepatocyte Nucleofector® Kit
method for primary rat hepatocytes in hand, you can now
VPL-1003 25 reactions
get a better understanding of new therapeutic agents and
toxicity mechanisms
– Up to 80% viability
– Suitable for both DNA and siRNA
– Maintenance of functional properties
A B
N
*
** *
N
N N
N
N = nuclei; * = bile canaliculi

Hepatocytes transfected by Nucleofection® maintain their morphology and
polarization. Primary rat hepatocytes were transfected by Nucleofection®
with the pmaxGFP® Vector (A) or a plasmid containing the cDNA sequence
for a plasma membrane receptor-YFP fusion protein (B). Cells were stained
with antibodies against desmoplakin (A; blue) to visualize cell boundaries
and against multidrug resistance protein 2 (MRP2; A+B; red) to show the
apical, canalicular membrane. maxGFP® Reporter Protein was located in the
cytosol of transfected cells (A). YFP-fusion protein was correctly targeted
to both the basolateral and the apical membrane domain as shown by
co-localization with MRP2 (B). These data prove normal formation of bile
canaliculi in hepatocytes transfected by Nucleofection®.
(Data courtesy of V. Keitel, F. Schliess and D. Häussinger, Department for
Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University
Düsseldorf, Germany.)
165
Mouse Astrocyte Nucleofector® Kit
Nucleofector® Kits
Neural Cells
Astrocytes play an important role in providing support Ordering Information

to neurons and controlling the structural and functional
Cat. No. Size Price
elasticity of synapses. They may be involved in the
pathogenesis of brain disorders. The Mouse Astrocyte Mouse Astrocyte Nucleofector® Kit
Nucleofector® Kit presents a powerful tool to gain further
VPG-1006 25 reactions
insights into the biological role and function of these
cells.
– Suitable for mouse astrocytes from cortex, midbrain
or striatum
– Maintenance of functionality
Nucleofection® of mouse astrocytes. Primary mouse astrocytes isolated

from mouse embryos (E14) were transfected by Nucleofection® using
the Mouse Astrocyte Nucleofector® Kit, program T-20/T-020 and a
plasmid encoding enhanced green fluorescent protein, eGFP. 2 days post
Nucleofection®, the cells were analyzed by fluorescence microscopy.
(Photograph courtesy of Dr. S. Franken, Dr. M. Eckhardt, Prof. V. Gieselmann,
Institute for Physiological Chemistry, University of Bonn, Germany.)
Rat Astrocyte Nucleofector® Kit
Astrocytes are essential for the communication between Ordering Information

neural cells and provide nutrients to the brain areas they
Cat. No. Size Price
serve. Apart from this knowledge, however, the function
of astrocytes remains a great mystery. The Nucleofector® Rat Astrocyte Nucleofector® Kit
Technology provides the ability to learn more about these
cells.
– Maintenance of functionality A B
Expression of GFAP and eGFP in rat astrocytes transfected by Nucleofection®.

Primary rat astrocytes were isolated from rat embryos (E17) and cultured C D
for 10 days until cells reached confluency. These cells were transfected
using the Rat Astrocyte Nucleofector® Kit, program T-20/T-020 and 5 µg of a
plasmid encoding the enhanced green fluorescent protein, eGFP. 24 hours
post Nucleofection®, cells were analyzed by fluorescence microscopy for
expression of eGFP (A) and GFAP (B), an astrocyte-specific marker protein.
Nuclei were stained with DAPI (C). Fig. D shows an overlay of the images.
(Photographs courtesy of Dr. Hyun-Ju Kim and Dr. Tim Vartanian, Beth Israel
Deaconess Medical Center, Dept. of Neurology, Boston, Massachusetts,
USA.)
166
Chicken Neuron Nucleofector® Kit
Nucleofector® Kits
Neural Cells
Chicken neurons are used as a model system to elucidate Ordering Information
neurological disorders, such as Parkinson’s Disease. The
Cat. No. Size Price
Nucleofector® Technology is providing a new insight that
will enable you to learn more about neurodegenerative Chicken Neuron Nucleofector® Kit
diseases using this in vitro model system.
– Transfection efficiencies of more than 40% VPG-1002 25 reactions
– Transgene expression for more than one week

– Suitable for hippocampal neurons and A B
dorsal root ganglia
– Maintenance of morphological and
functional properties
C
Formation of normal growth cones indicates maintenance of functionality
of dorsal root ganglia after Nucleofection®. DRG neurons from chicken were
transfected by Nucleofection® with a plasmid encoding the GFP protein.
After cultivation on pre-coated glass coverslips overnight, single cells were
analyzed for formation of normal growth cones (A-C), F-actin localization
after staining with Alexa 568 conjugated phalloidin (A and C) and GFP
expression (B and C).
(Photograph courtesy of B. Eickholt, King’s College, London, Great Britain.)
Mouse Neuron Nucleofector® Kit
Mouse neurons are highly interesting scientifically Ordering Information

as they are used to develop new therapies for severe
Cat. No. Size Price
disorders such as Alzheimer’s or Parkinson’s Diseases.
The Nucleofector® Technology offers the first non-viral Mouse Neuron Nucleofector® Kit
method for efficient gene transfer into primary mouse
neurons. VPG-1001 25 reactions
– Up to 60% transfection efficiencies

– Maintenance of morphological and functional
properties
A B
Nucleofection® of primary mouse hippocampal neurons. Primary dissociated

neurons of mixed glial cultures were transfected using the Mouse Neuron
Nucleofector® Kit and a plasmid encoding the enhanced green fluorescent
protein eGFP. 48 hours post Nucleofection®, the cells were analyzed by light
(A) and fluorescence microscopy (B).
(Photograph courtesy of A. Dityatev, G. Dityateva and M. Hammond, Center
for Molecular Neurobiology, Hamburg, Germany.)
167
Rat Neuron Nucleofector® Kit
Nucleofector® Kits
Neural Cells
Rat neurons are frequently used as an experimental model Ordering Information

to study neural cell function and to develop new therapies
Cat. No. Size Price
for neurodegenerative diseases. The Nucleofector®
Technology offers the first non-viral method for efficient Rat Neuron Nucleofector® Kit
gene transfer into primary rat neurons.
– Transfection efficiencies of up to 60% VPG-1003 25 reactions

– Protocols for hippocampal neurons, cortical neurons
and dorsal root ganglia neurons
– Maintenance of morphological and functional Vector miR1-1 miR132
properties
– Proven performance for siRNA, shRNA, miRNA, and
antisense oligos
Expression of miR132 induces neurite sprouting by targeting a protein that

represses neurite outgrowth (p250GAP). Rat neonatal cortical neurons
were transfected with a GFP reporter (green) and co-transfected with vector
control, or expression constructs for premiR1-1 or premiR132 using the Rat
Neuron Nucleofector® Kit. Cells were immunostained for the neuronal marker
MAP2 (red). Only cells transfected with premiR132 show neurite sprouting.
(Vo et al., reproduced from the Proc Natl Acad Sci USA, 102(45): 16429 by
copyright of the National Academy of Science and by permission of the
authors.)
Rat Neuron 96-well Nucleofector® Kit
Rat neurons are a versatile in vitro model system to study Ordering Information
neural cell function and interaction of neurons in the
Cat. No. Size Price
nervous system. Efficient high throughput transfection
of these cells is a key issue to elucidate neurolo- Rat Neuron 96-well Nucleofector® Kits
gical pathways. With the Rat Neuron 96-well Nucleofector®
Kit, this gap is closed. High throughput Nucleofection® of VHPG-1003 96 reactions
rat neurons offers up to 50% transfection efficiency. VHPG-2003 960 reactions
A B
➔
DIV = Days in vitro
96-well Nucleofection® of neonatal rat hippocampal neurons. Freshly Normal neuronal development after 96-well Nucleofection®. Freshly isolated
isolated rat cells (P0) were transfected by Nucleofection® with program neonatal rat hippocampal neurons (P0) transfected by Nucleofection® with
96-CU-133 and pSyn-GFP (eGFP under control of the synapsin promoter). pSyn-GFP and pDsRed were plated onto glass coverslips and examined
After 7 DIV, neurons were fixed and analyzed by light (A) and fluorescence after 7 DIV for GFP (green) and RFP (red) expression. Transfected neurons
(B) microscopy. Neuron morphology was unaltered compared to non- show an extensive dendritic network and develop dendritic protrusions that
transfected neurons. already resemble mature mushroom shaped dendritic spines (arrows).
Surrounding, transfected glia cells are shown by DAPI staining (blue).
(Data courtesy of M. Kiebler, Department of Neuronal Cell Biology, Medical
University of Vienna, Vienna, Austria.)
168
Rat Oligodendrocyte Nucleofector® Kit
Nucleofector® Kits
Neural Cells
During multiple sclerosis, oligodendrocytes as well as Ordering Information
the myelin sheath which insulates axons, are destroyed.
Cat. No. Size Price
Nucleofector® Technology is the first technology which
permits an efficient non-viral gene transfer into these Rat Oligodendrocyte Nucleofector® Kit
cells. This provides further insights into oligodendrocyte
biology and dysfunction. VPG-1009 25 reactions

– Maintenance of functionality A B
Nucleofection® of rat oligodendrocytes. Primary rat oligodendrocyte

precursor cells were transfected by Nucleofection® using the Rat C
Oligodendrocyte Nucleofector® Kit, program O-17 and 1 µg of a plasmid
encoding the enhanced green fluorescent protein eGFP (A) or 0.25 µg of a
plasmid encoding a histone 2B-tagged enhanced green fluorescent protein
eGFP (B) which is predominantly located in the nucleus. 3 days post
Nucleofection®, cells were analyzed by fluorescence microscopy. In figure
(C) same cells were stained for the oligodendrocyte-specific marker MBP.
(Photograph courtesy of W. Deng, Children‘s Hospital, Boston, Massachusetts,
USA (A) and H. Colognato, Dept. of Pathology, Univ. of Cambrige, UK (B, C).)
Basic Nucleofector® Kit for Primary Mammalian Neurons

Neurons is the ideal tool to transfect a multitude of
Cat. No. Size Price
neuronal cells from different mammalian species (e.g.,
mouse, rat, human) and from various origins (e.g., Basic Nucleofector® Kit for Primary Mammalian Neurons
cerebrellum, cortex, hippocampus).
– Suitable for primary mammalian neural cells VPI-1003 25 reactions

Nucleofector® Solution necessary
– Successful transfection of rat neural progenitor cells,
cow cromaffin cells, etc.
100
DsRed
EGFP
90
80
Comparison of conventional electroporation, lipofection and Nucleofection® 70
for transfection of rat neuronal progenitor cells. Ventral mesencephalic
progenitor (VMP) cells from rat brain, which are the important source 60
of dopaminergic neurons for cell replacement strategies in Parkinson’s 50
Disease, were transfected with two different plasmids expressing DsRed 40
or EGFP. For transfection, conventional electroporation (EasyjecT from 30
EquiBio, 100 µg plasmid per 500,000 cells), lipofection (Lipofectamine™
20
2000 Reagent, 0.5 µg DNA per 60,000 cells), or Nucleofection® (5 µg DNA
per 2,000,000 cells) were used. 10
(Data from Cesnulevicius et al. (2006) Stem Cells 24(12), 2776-91.) 0 Electroporation Lipofection Nucleofection®
169
Basic 96-well Nucleofector® Kit for Primary Mammalian Neurons
Nucleofector® Kits
Neural Cells
Primary mammalian neurons are a versatile tool to study Ordering Information

the physiology, the interaction and the regenerative
Cat. No. Size Price
capacities of neuronal cells. The Basic 96-well
Nucleofector® Kit for primary mammalian neurons allows Basic 96-well Nucleofector® Kits for
the transfection of a multitude of neuronal cells from Primary Mammalian Neurons
different mammalian species like mouse, rat, human,
and different tissue origins at high throughput. Moreover, VHPI-1003 96 reactions
the unique Nucleocuvette® Plate guarantees the most VHPI-2003 960 reactions
efficient transfection of these precious cells by reducing

the cell number per transfection down to 5 x 104 cells.
– Testing of just 7 selected programs with one
Nucleofector® Solution necessary
(within one Nucleocuvette® Module)
– Optimize Nucleofector® Program and cell number in
one experiment
– 80% fewer cells required compared to standard
– Maintenance of morphological and functional
properties
The Approach
1. 2. 3. 1. Choose your primary mammalian

neuron of interest.
Program ProgramTransfection
Program Transfection
Transfection
2. Test one 96-well Nucleofector® Solution
efficiency (%)
efficiency (%)
efficiency (%) in combination with seven different
96-CA-13896-CA-13896-CA-138
10 10 10 96-well Nucleofector® Programs. You
96-CL-13396-CL-13396-CL-133
40 40 40 can also titrate different cell numbers.
96-CU-11096-CU-11096-CU-110
50 50 50 3. Identify the optimal Nucleofector®
96-DC-10496-DC-10496-DC-104
50 50 50 > > > > > > > > > 96-well Program (and additionally,
96-DR-11496-DR-114 35
96-DR-114 35 35 the optimal cell number) for best
transfection performance.
96-EM-11096-EM-11096-EM-110
40 40 40
170
Basic Neuron SCN Nucleofector® Kit
Nucleofector® Kits
Neural Cells
The Basic Neuron Small Cell Number (SCN) Nucleofector® Ordering Information
Kit makes high efficiency transfection of mammalian
Cat. No. Size Price
primary neurons possible for extremely low cell numbers.
Now you can transfect just the number of cells needed Basic Neuron SCN Nucleofector® Kit
for later culture and analysis (e.g. microscopic analysis
VSPI-1003 25 reactions
of neuron growth and morphology). Benefit from a drastic
reduction in both number of donor animals and amount
of preparation time. The new, optimized SCN Nucleofector®
Solution, SCN Cuvettes and Programs make transfection
efficiencies of up to 60% possible with down to 15,000
cells per Nucleofection®.
– Transfection of down to 15,000 cells per Nucleofection®
(i.e., more experiments with fewer donor animals)
– Excellent non-viral transfection efficiencies
and viabilities (i.e., 50% and higher even for very
difficult-to-transfect neurons like DRGs)
A B
SCN Nucleofection® of freshly isolated mouse dorsal root ganglion (DRG)

cells. 15,000 DRGs (E15.5) were transfected with SCN Basic Neuro Program
6 and 0.4 µg pmaxGFP® Vector (green, B) and seeded on a coated coverslip.
24 hours post Nucleofection®, cells were fixed and immunostained for
beta-tubulin III (red, neuronal marker, A). In this experiment, transfection
efficiency as determined by fluorescence microscopy was approximated
at 60%.
(Data courtesy of B. Eickholt, MRC Centre for Developmental Neurobiology,
King‘s College, London, United Kingdom.)
171
Human Aortic Smooth Muscle Cell Nucleofector® Kit
Smooth Muscle Cells
Nucleofector® Kits
Aortic smooth muscle cells (AoSMC) are a well Ordering Information

established cell system that enables studies on human
Cat. No. Size Price
vascular disorders, such as atherosclerosis and stroke.
As a result of the slow proliferation rate of aortic smooth Human Aortic Smooth Muscle Cell Nucleofector® Kit
muscle cells, the expression of a transiently transfected
VPC-1001 25 reactions
gene can be monitored for up to three weeks (see
below).
– 10-fold higher transfection efficiency compared
to lipofection
– For aortic and vascular smooth muscle cells
– Suitable for transient long-term expression
A B
Example for the transfection of human AoSMC with eGFP. Human AoSMC
were transfected by Nucleofection® using the Human AoSMC Nucleofector®
Kit, program U-25/U-025 and a plasmid encoding the enhanced green
fluorescent protein eGFP. 14 hours post Nucleofection®, the cells were
analyzed by light (A) or, fluorescence microscopy (B).
Expression kinetics for human AoSMCs

100
% Transfected cells
90
80
70
60
50
40
30
20
10
0 1d 2d 7d 14 d 21 d 28 d
Time course of transient expression of transfected human AoSMC. Human

AoSMC were transfected by Nucleofection® using the Human AoSMC
Nucleofector® Kit and a plasmid encoding the mouse MHC class I heavy
chain molecule H-2Kk. 1, 2, 7, 14, 21, and 28 days post Nucleofection®, the
cells were analyzed for their H-2Kk expression by flow cytometry. Dead cells
were excluded from the analysis by propidium iodide staining and gating.
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AoSMC - Aortic Smooth Muscle Cells 65
SmGM®-2 BulletKit® 65
172
Basic Nucleofector® Kit for Primary Smooth Muscle Cells
Smooth Muscle Cells

Nucleofector® Kits
Smooth Muscle Cells (SMCs) is the ideal tool to transfect
Cat. No. Size Price
a multitude of SMCs from different mammalian species
(e.g., mouse, rat, human) and from various organs (e.g., Basic Nucleofector® Kit for Primary Smooth Muscle Cells
blood vessels, intestine).
Example for Nucleofection® of Primary Porcine Vascular Smooth Muscle

Cells. Primary porcine vascular smooth muscle cells were transfected by
Nucleofection® with the Basic Nucleofector® Kit for Primary Smooth Muscle
Cells, program A-33/A-033 and a plasmid encoding the green fluorescent
maxGFP® Reporter Protein. 48 hours post Nucleofection®, the cells were Related Products
analyzed by fluorescence microscopy. A transfection efficiency of around
80 - 90% was achieved.
(Data courtesy of Dr. TaoWang and Dr. CathyM. Holt, University of Manchester, Smooth Muscle Cell Systems 64 – 68
United Kingdom.) SmGM®-2 BulletKit® 65
Basic 96-well Nucleofector® Kit for Primary Smooth Muscle Cells
Smooth muscle cells (SMCs) can be found within the tunica Ordering Information
media layer of arteries and veins, the bladder, uterus,
Cat. No. Size Price
male and female reproductive tracts, gastrointestinal
tract, respiratory tract, the ciliary muscle, and iris of Basic 96-well Nucleofector® Kits for
the eye. Vascular smooth muscle cells represent a Primary Smooth Muscle Cells
well established system to study vascular disorders
such as atherosclerosis and stroke. The Basic 96-well VHPI-1004 96 reactions
Nucleofector® Kit for Primary Smooth Muscle Cells allows VHPI-2004 960 reactions
high throughput Nucleofection® with almost any type of
mammalian SMCs.
A B
Nucleofection® of primary smooth muscle cells. Primary pulmonary artery Related Products
smooth muscle cells were transfected with 0.2 µg the pmaxGFP® Vector
using the Basic 96-well Nucleofector® Kit for Primary Smooth Muscle
Cells. 24 hours post Nucleofection®, cells were analyzed by light (A) and Smooth Muscle Cells & Media 64 – 68
fluorescence (B) microscopy. SmGM®-2 BulletKit® 65
173
Human Stem Cell Nucleofector® Kit
Nucleofector® Kits
Stem Cells
Embryonic stem cells (ES cells) are derived e.g. from the Ordering Information
inner cell mass of preimplantation embryos and retain the
Cat. No. Size Price
developmental potency of embryonic founder cells, being
able to differentiate into cells and tissues of all three germ Human Stem Cell Nucleofector® Starter Kit
layers in vitro and in vivo. Since ESCs can be differentiated
into many cell types in vitro, they provide a resource not VPH-5002 18 reactions
only for studying basic human developmental biology, Human Stem Cell Nucleofector® Kit 1
but also for therapeutic clinical approaches to replace
VPH-5012 25 reactions
diseased cells in humans.
– Tedious creation of viruses unnecessary Human Stem Cell Nucleofector® Kit 2
– Higher transfection efficiencies compared
to other methods
– Less DNA and lower cell number needed
100 Efficiency
Transfection efficiency / viability (%)
Viability
90
80
70
60
50
40
30
20
10
0 H1 H9 H 9.2 Hues-9 BG01V Customer
Average transfection efficiency of human stem cell lines. Different human

stem cell lines were transfected by Nucleofection® using the pmaxGFP®
Vector according to the guidelines given in the human Stem Cell Nucleofector®
Starter Kit. (Data for Nucleofection® of human stem cells are compiled from
experiments performed by leading stem cell research customers.)
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NHNP - Normal Human Neural Progenitors Cells 97
174
Basic 96-well Nucleofector® Kits for Human Stem Cells
Nucleofector® Kits
Stem Cells
Human embryonic stem cells are the subject of Ordering Information
increasing scientific interest because of their potential
Cat. No. Size Price
utility in numerous biomedical applications. Since stem
cells are a self-renewing population of cells, they can Basic 96-well Nucleofector® Starter Kit for
be continuously cultured in an undifferentiated state Human Stem Cells
and give rise to more specialized cells of the body, such
VHPH-5002 64 reactions
as heart, liver, fat, blood and nerve cells. The new Basic
96-well Nucleofector® Kit for Human Stem Cells now Basic 96-well Nucleofector® Kit 1 for Human Stem Cells
allows high throughput Nucleofection® of other human
embryonal stem cell lines combined with the ability to use
low cell numbers.
– Economical starter kit for fast determination of ideal Basic 96-well Nucleofector® Kit 2 for Human Stem Cells
Nucleofection® conditions VHPH-5022 96 reactions
– Successfully tested for H1, H9, H14 and HS306 cells VHPH-5222 960 reactions
A B
Example for Nucleofection® of human embryonic stem cells. Human

embryonic HS306 cells were transfected with the pmaxGFP® Vector
using the Basic 96-well Nucleofector® Kit for Human Stem Cells. 24 hours
post Nucleofection®, cells were analyzed by light (A) and fluorescence
microscopy (B).
(Data courtesy of Jennifer Moore, Rutgers University, Piscataway, USA.)
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NHNP - Nomal Human Neural Progenitors Cells 97
175
Human Stem Cell (H9) 96-well Nucleofector® Kit
Nucleofector® Kits
Stem Cells
The genetic manipulation of human stem cells has Ordering Information

been difficult due to their resistance to high efficiency
Cat. No. Size Price
transfection. With the release of Nucleofector® Kits
dedicated to transfect these cells, this bottleneck has Human Stem Cell (H9) 96-well Nucleofector® Kit
been overcome. Now, the new Human Stem Cell (H9)
96-well Nucleofector® Kit allows high performance VHPH-5003 96 reactions
Nucleofection® of the stem cell line H9 in a 96-well format, VHPH-5203 960 reactions
thus enabling high throughput approaches to elucidate
various aspects of stem cell differentiation.
– 64% transfection efficiency
– Excellent preservation of pluripotency
H9 cells preserve pluripotency post Nucleofection®. H9 cells transfected

with the pmaxGFP® Vector maintain their undifferentiated state. Analysis
after 24 hours shows expression of GFP (green) as well as of the
pluripotency markers SSEA4 (red) and Oct4 (purple). The blue signals refer
to nuclear staining by DAPI.
(Data kindly provided by Jennifer Moore, Rutgers University, Piscataway, USA.)
Human CD34+ Cell Nucleofector® Kit
In their role as hematopoietic progenitors, CD34+ cells Ordering Information

are of great interest to immunologists. They have been
Cat. No. Size Price
shown to differentiate into other non-hematopoietic cells,
such as endothelial precursors. So far, transfection of Human CD34+ Cell Nucleofector® Kit
CD34+ progenitor cells has only been possible with viral
methods or by electroporating stimulated cells. Using VPA-1003 25 reactions
Nucleofection®, unstimulated CD34+ cells can now be

transfected non-virally. For Nucleofection®, CD34+ cells
can be derived from cord blood or leukapheresis material. Peripheral Blood Stem Cells Bone Marrow Stem Cells
Both fresh or cryopreserved material can be used. 50 50
% Positive cells
% Positive cells
– Up to 70% transfection efficiency 40 40
– No influence on hematopoietic cell differentiation 30 30

20 20
10 10
– For unstimulated primary CD34+ cells 0 4h 36 h 84 h 120 h 200 h 0 4h 36 h 84 h 120 h 200 h
Time Time
Long-term transgene expression after Nucleofection® in blood and bone

marrow derived CD34+ cells. Kinetics of deltaLNGFR (Low Affinity Nerve
Growth Factor Receptor) expression were determined by flow cytometric
analysis. 39 ± 5.9% of peripheral blood stem cells showed deltaLNGFR
staining 4 hours after transfection with a continuous decrease (n = 3, 3
patients). Bone marrow stem cells showed maximal deltaLNGFR expression
with 26 ± 9.7% 84 hours after transfection, which then decreased in the
proliferating culture (n = 3, single patient).
(Data courtesy of Greiner et al., University Hospital of Ulm, Ulm, Germany.)
176
Mouse ES Cell Nucleofector® Kit
Nucleofector® Kits
Stem Cells
Mouse ES cells offer the ability to differentiate into various Ordering Information
cell types and tissues, enabling them to be employed for
Cat. No. Size Price
cell differentiation studies or the generation of knock-out
mice. Mouse ES Cell Nucleofector® Kit
– Tested with several mouse ES cell lines
(e.g., R1, D3, E14)
– Successfully used to generate germline chimeras
– Homogenous transient gene expression pattern
– No influence on in vitro or in vivo A-13 no DNA EP
differentiation potential
Comparison of Nucleofection® and electroporation for transfection of mouse

ES cells. Mouse ES cells were transfected by Nucleofection® using program
A-13/A-013 and compared to mock-transfected (no DNA) and electroporated
(EP) ES cells using Bio-Rad® Gene Pulser®. Cells were stained 48 hours after
transfection for transient lacZ expression.
(Data courtesy of S. Boljahn, A. Rode, M. Joao da Silva, T. Hennek and B.
Zevnik, Artemis Pharmaceutical GmbH, Cologne, Germany.)
Mouse ES Cell 96-well Nucleofector® Kit
Mouse ES cells are often used to study the biological Ordering Information
basics of cell differentiation, tissue formation and embryo
Cat. No. Size Price
development. In addition, transfected Mouse ES cells are
used to generate knock-out mice. Mouse ES Cell 96-well Nucleofector® Kit
– Viability of up to 80% after 24 hours VHPH-1001 96 reactions
– Preservation of cell functionality VHPH-2001 960 reactions
(ability to differentiate)
100 Transfection efficiency
Transfection efficiency / viability (%)
Viability
90
80
70
60
50
40
30
20
10
0 E14 D3
Efficiency and viability of the Mouse ES Cell 96-well Nucleofector® Kit.

5 x 104 cells were transfected by Nucleofection® using 0.4 µg pmaxGFP®.
Cells were analyzed 24 hours post Nucleofection® for GFP expression and
viability using flow cytometry. Cell viability is given in percent compared to
non-transfected control.
177
Human MSC (Mesenchymal Stem Cell) Nucleofector® Kit
Nucleofector® Kits
Stem Cells
The ability of bone marrow derived MSCs to differentiate Ordering Information

into various cell types has generated enormous interest
Cat. No. Size Price
in many different research fields. For the first time, an
efficient non-viral transfection of these cells is possible Human MSC (Mesenchymal Stem Cell) Nucleofector® Kit
through the use of the Human MSC Nucleofector® Kit.
VPE-1001 25 reactions
– Maintenance of functional properties
100 Efficiency
Viability
90
80
70
60
50
40
30
20
10
0 Nucleofection® FUGENE® 6 DOTAP
MSCs were transfected by Nucleofection® with 2 µg pcDNA3/NT-GFP using

either Nucleofection® (Human MSC Nucleofector® Kit and program U-023) or
the lipid-based Fugene® 6 or DOTAP Reagents (both Roche Applied Science).
MSCs transfected by Nucleofection® were analzyed for transfection
efficiency roughly 60 hours post Nucleofection®, cells transfected with
Fugene® 6 or DOTAP Reagents were analyzed after 72 hours. Transfection
efficiency was scored by flow cytometric analysis and reported as
percentage of GFP+ cells. The percentage of viable cells was estimated by
trypan blue exclusion.
(Data courtesy of Aluigi M, Fogli M, Curti A, Isidori A, Gruppioni E, Chiodoni C,
Colombo MP, Versura P, D’Errico-Grigioni A, Ferri E, Baccarani M and Lemoli
RM, Institute of Hematology and Medical Oncology, Bologne, Italy.)
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MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit® 90
178
Mouse NSC (Neural Stem Cell) Nucleofector® Kit
Nucleofector® Kits
Stem Cells
NSC from mouse brain can be differentiated into various Ordering Information
neural and glial cell types. This may potentially contribute
Cat. No. Size Price
to the development of therapies for CNS disorders via
replacement of damaged neural tissue. The Nucleofector® Mouse NSC (Neural Stem Cell) Nucleofector® Kit
Technology presents a powerful tool to gain further
insights into how these cells function.
– Up to 90% viability
– Transgene expression for several days A B
– Differentiation into neurons and astrocytes possible
Nucleofection® of Mouse NSCs. Primary Mouse NSCs isolated from the

lateral ventrical wall of an adult mouse were transfected by Nucleofection®
using the Mouse NSC Nucleofector® Kit, program A-33/A-033 and a plasmid
encoding the enhanced green fluorescent protein eGFP. 48 hours post
Nucleofection®, the cells were analyzed by light (A) and fluorescence
microscopy (B).
(Photograph courtesy of Dr. L. Wikstrom et al., NeuroNova, Stockholm,
Sweden.)
Rat NSC (Neural Stem Cell) Nucleofector® Kit
The use of Rat NSCs in research will lead to a better Ordering Information
understanding of normal brain development. Additionally,
Cat. No. Size Price
neural cells derived from NSCs have the ability to replace
damaged neurons or degenerate tissue. The Nucleofector® Rat NSC (Neural Stem Cell) Nucleofector® Kit
Technology offers the first non-viral method for efficient
gene transfer into primary neural stem cells. VPG-1005 25 reactions
– More than 40% transfection efficiency

– Transgene expression for several days A B
– Suitable for both neurospheres and adherent cells
– Differentiation into neurons and astrocytes possible
Nucleofection® of Rat NSCs. Primary NSCs isolated from rat embryos (E14)
were transfected by Nucleofection® using the Rat NSC Nucleofector® Kit,
program A-31/A-031 and a plasmid encoding enhanced green fluorescent
protein eGFP under control of an EF1alpha promoter (pcDNAEF1-eGFP).
Post Nucleofection®, cells were cultured with bFGF for 2 days, then for
5 additional days without bFGF to differentiate into neurons. Cells were
analyzed 2 days (A) and 7 days (B) post Nucleofection® by fluorescence
microscopy.
(Photograph courtesy of S.H. Lee, College of Medicine, Dept. of Biochemistry,
Hanyang University, Seoul, South Korea.)
179
Rat Cardiomyocyte – Neonatal Nucleofector® Kit
Rat Cardiomyocytes
Nucleofector® Kits
The efficient introduction of DNA and RNAi substrates Ordering Information

into cardiomyocytes is of great importance in the
Cat. No. Size Price
molecular characterization of cardiac muscle function
and development. The lack of satisfactory non-viral Rat Cardiomyocyte - Neonatal Nucleofector® Kit
transfection methods for cardiac cells, such as neonatal
VPE-1002 25 reactions
rat cardiomyocytes, was a major obstacle in cardio-
vascular research. With Nucleofector® Technology, you
can now achieve efficient transfection and address areas
such as cardiac gene regulation and differentiation and
the characterization of functional cardiac proteins.
– Ideal for neonatal rat cardiomyocytes
– First efficient non-viral transfection technology
– Includes detailed cell isolation protocol
A B
Example for Nucleofection® of neonatal rat cardiomyocytes with DsRed2

cDNA. Primary neonatal rat cardiomyocytes were transfected by
Nucleofection® using the Rat Cardiomyocyte - Neonatal Nucleofector® Kit,
program G-09/G-009 and 4 µg of a plasmid encoding DsRed [Clontech].
2 days post Nucleofection®, the cells were analyzed by fluorescence
microscopy. Fig. (A) shows DsRed expressing cells. Cardiomyocytes stained
with FITC-labeled tropomyosin antibody are shown in fig. (B). Fig. (C) is an
overlay of images (A) and (B).
(Photograph courtesy of F. Engel and M. Keating, Cardiology Department,
Children’s Hospital, Havard Medical School, Boston, Massachusetts, USA.)
180
Human Chondrocyte Nucleofector® Kit
Human Chondrocytes
Nucleofector® Kits
The study of chondrocytes, the matrix-forming cells of Ordering Information
cartilage, is of major importance in tissue engineering and
Cat. No. Size Price
regenerative medicine. Nucleofection® of chondrocytes
allows for a better understanding of the basic principles Human Chondrocyte Nucleofector® Kit
of degenerative processes such as osteoarthritis.
VPF-1001 25 reactions
– First efficient non-viral transfection technology
Example of the transfection of human chondrocytes with eGFP. Human

chondrocytes were transfected by Nucleofection® using the Human
Chondrocyte Nucleofector® Kit, program U-24/U-024 and 5 µg of a plasmid
encoding the enhanced green fluorescent protein eGFP. Cell membranes were
fluorescently stained in red with the substance R18 (Octadecylrhodamine-
B-chloride, Molecular Probes). 24 hours post Nucleofection®, the cells were
analyzed by fluorescence microscopy. The image shows an overlay of eGFP
and R18 fluorescence.
(Data courtesy of Dr. Schmid and Dr. Aigner, University of Erlangen, Germany.)
Human Chondrocyte 96-well Nucleofector® Kit
It is of vital interest for regenerative medicine and tissue Ordering Information

engineering to decipher the functionality of human
Cat. No. Size Price
chondrocytes. High throughput Nucleofection® with the
Human Chondrocyte 96-well Nucleofector® Kit marks a Human Chondrocyte 96-well Nucleofector® Kits
milestone for this scientific challenge. It allows functional
studies of genes involved in degenerative processes and VHPF-1001 96 reactions
cartilage formation. VHPF-2001 960 reactions
A B
Example showing typical Nucleofection® results of human chondrocytes.

Primary human chondrocytes were transfected with 1 µg pmaxGFP®
Vector usíng the Human Chondrocyte 96-well Nucleofector® Kit. 24 hours
post Nucleofection®, cells were analyzed by light, A, and fluorescence
microscopy, B.
(Pictures by courtesy of Dr. Jochen Haag, University of Leipzig.)
181
MEF (Mouse Embryonic Fibroblast) Nucleofector® Kit
Nucleofector® Kits
MEFs display significant genotypic and phenotypic Ordering Information

MEF
variations depending on the strain and the genetic

Cat. No. Size Price
background of the mice they were isolated from, as well
as the immortalization strategy used. Some MEF cell MEF Starter Nucleofector® Kit
types were impossible to transfect as each could behave
differently with respect to the optimal conditions for
efficient transfection. Lonza has resolved this drawback MEF 1 Nucleofector® Kit
by developing two MEF Nucleofector® Kits (1 and 2) and
an easy-to-use MEF Starter Nucleofector® Kit.
– Fast and individual Nucleofection® of your MEF cells MEF 2 Nucleofector® Kit
– Two different MEF kits (1 and 2) to address genotypic VPD-1005 25 reactions

and phenotypic variations
– MEF Starter Kit for fast determination of ideal
Nucleofection® Conditions
– Up to 50% transfection efficiency after 24 hours
Example of Nucleofection® of primary MEFs. Spontaneously immortalized

mouse embryonic fibroblasts (strain: C57BL/6 x 129Sv) were transfected
by Nucleofection® using the MEF Nucleofector® Kit 1 and a plasmid encoding
the enhanced green fluorescent protein eGFP. 24 hours post Nucleofection®,
the cells were analyzed by fluorescence microscopy.
(Photograph courtesy of Dr. H. Hermanns and Prof. P.H. Heinrich, University
of Aachen, Germany.)
182
Optimized Protocols for Cell Lines
Optimized Protocols
for Cell Lines
NF: An Optimized Protocol is available using the Cell Type Optimized Transfection Viability
Amaxa® Nucleofector® Device Protocol Efficiency
96: An Optimized Protocol is available using the 293 NF 84%
Amaxa® 96-well Shuttle® System 293 96 83% 93%
32D NF 79% 61%
3T3-L1 ad NF 25% 90%
3T3-L1 pre-ad NF 73% 59%
A-10 NF 64% 74%
A-375 NF 72% 97%
A-431 NF 45% 83%
A20 NF 74% 81%
A2058 NF 81% 94%
A549 NF 72% 81%
A7r5 NF 49% 81%
AGS NF 73% 62%
ARPE-19 NF 83% 92%
ARPE-19 96 87%
B16-F0 NF 84% 91%
B16-F10 NF 91% 96%
BA/F3 NF 88% 79%
BHK-21 NF 85% 78%
BJ NF 52% 76%
BxPC-3 NF 28% 62%
C2C12 NF 82% 93%
C6 NF 70 – 94% 80%
Caco-2* NF 59% 70%
Capan-1 NF 29% 78%
CCRF-CEM NF 68% 79%
CHO [suspension] NF 84 – 92% 82%
CHO-K1 NF 94% 53 – 87%
CHO-K1 96 86% 97%
CHO-S [suspension] NF 90 – 98% 67 – 72%
CHO-S [suspension] 96 84 – 86% 55 – 57%
COS-1 NF 49% 64%
COS-7 NF 99% 94%
COS-7 96 91 – 99% 80 – 96%
D1 ORL UVA NF 61% 97%
DU 145 NF 47% 89%
EL4 NF 65% 76%
EL4 96 70 – 80%
FDC-P1 NF 82% 84%
GH3 NF 77% 84%
GH3 96 60 – 80% 60 – 70%
H9c2(2-1) NF 86% 90%
HaCaT NF 43%
HCT 116 NF 78% 76%
HCT 116 96 70 – 80% 65 – 75%
*Caco-2 was developed by the Memorial Sloan Kettering Cancer Center
and is accordingly subject to certain third party rights and commercial HeLa NF 70%
use restrictions. Please contact Memorial Sloan Kettering Cancer Center or HeLa 96 75% 89%
Navicyte Scientific for licensing information.
183
Optimized Protocols for Cell Lines
Optimized Protocols
Continued
for Cell Lines
NF: An Optimized Protocol is available using the Cell Type Optimized Transfection Viability
Amaxa® Nucleofector® Device Protocol Efficiency
96: An Optimized Protocol is available using the Neuro-2a [N2a] NF 76% 79%
Amaxa® 96-well Shuttle® System Neuro-2a [N2a] 96 67% 82%
NG108-15 NF 64% 82%
NIH/3T3 NF 84% 87%
NIH/3T3 96 95% 93%
NK-92 NF 26% 40%
Cell Type Optimized Transfection Viability NRK NF 44% 91%

Protocol Efficiency NS0 NF 83% 54%
HeLa S3 NF 67% 95% NTERA-2 cl.D1 NF 90% 94%
HeLa S3 96 61 – 85% 62 – 95% P19 NF 85% 80%
Hep G2 NF 41 – 64% 86 – 94% P815 NF 62%
Hep G2 96 96% 93% PANC-1 NF 68% 75%
HL-60 NF 50 – 90% 50 – 65% PC-12 NF 92% 81%
HL-60 96 58% 61% PC-3 NF 81 – 88% 59 – 66%
HT-1080 NF 74% 76% Raji NF 84% 81%
HT-29 NF 16 – 51% 57 – 94% Ramos NF 27% 72%
HuT 78 NF 53% 64% Ramos 96 20 – 30% 70 – 86%
HUV-EC-C NF 75% 77% RAW 264.7 NF 65% 74%
IMR-32 NF 80% 62% RAW 264.7 96 60% 86%
IMR-90 NF 51% 70% RBL-1 NF 83% 67%
Jurkat NF 84 – 88% 75 – 90% RBL-2H3 NF 42% 93%
Jurkat 96 71 – 92% 71 – 80% S49 NF 81% 68 – 95%
K-562 NF 89% 88% Saos-2 NF 82% 79%
K-562 96 92% 95% Schneider's Drosophila Line 2 NF 76% 70%
KG-1 NF 70% 84% Sf9 NF 59 – 82% 76 – 79%
KG-1a NF 86% 79% SH-SY5Y NF 63 – 82% 40%
L-428 NF 78% 73% SH-SY5Y 96 81% 80%
L-428 96 70 – 80% 85% SK-BR-3 NF 50% 94%
L6 NF 59% 92% SK-N-SH NF 85% 73%
LNCaP NF 82% 80% SK-OV-3 NF 89% 53%
MCF7 NF 77% 60% SW480 NF 60% 86%
MCF7 96 72% 89% T-47D NF 51% 94%
MDA-MB-231 NF 79% 77% T-47D 96 80%
MDA-MB-231 96 73 – 89% T/G HA-VSMC NF 58% 79%
MDA-MB-453 NF 54% 90% T2 NF 60% 68%
MDA-MB-468 NF 60% 81% T84 NF 52% 83%
MDCK NF 73% 83% TF-1 NF 38% 82%
MDCK 96 80 – 99% 80 – 99% THP-1 NF 47 – 68% 40 – 58%
MDCK II NF 80% 88% THP-1 96 65% 81%
MEG-01 NF 80% 66% U-2 OS NF 98% 88%
MG-63 NF 62% 90% U-87 MG NF 43% 91%
MOLT-4 NF 55% 61% U-937 NF 67% 85%
MV-4-11 NF 29% 79% U-937 96 36% 85%
NALM-6 NF 64% 87% U266B1 NF 86% 91%
NB-4 NF 71% 66% Vero NF 79% 97%
NCI-H1299 [H1-299] NF 99% 75% WEHI-231 NF 77% 62%
NCTC clone 929 NF 67% 91% WI-38 NF 75% 91%
184
Nucleofection® of Cell Lines
Nucleofection® of
Cell Lines
For the transfection of cell lines, Lonza offers five different Ordering Information
Amaxa® Cell Line Nucleofector® Solutions: C, L, R, T, and V.
Cat. No. Size Price
Optimized Protocols outlining the optimal Nucleofector®
Kit for a large selection of cell lines are already available Cell Line Nucleofector® Kit C
and can be downloaded from www.lonza.com/celldatabase.
VCA-1004 25 reactions
Lonza’s R&D Team is continuously optimizing the
Nucleofection® Protocols for new cell. Make sure you are Cell Line Nucleofector® Kit L
up-to-date with our latest developments by visiting our
cell line database.
– Achieve transfection efficiencies of up to 90% with Cell Line Nucleofector® Kit R
high cell viability VCA-1001 25 reactions
– Up to 99% transfection efficiency with siRNA duplexes Cell Line Nucleofector® Kit T
even in suspension cells
– Expression within hours - from transfection to
analysis in a day Cell Line Nucleofector® Kit V
High Throughput Nucleofection® of Cell Lines
For the high throughput transfection of cell lines using Ordering Information
the 96-well Shuttle® System, Lonza offers three different
Cat. No. Size Price
Amaxa® Cell Line 96-well Nucleofector® Kits: SE, SF
and SG which are available in two different sizes (96 Cell Line 96-well Nucleofector® Kit SE
and 960 reactions). Optimized Protocols outlining the
VHCA-1001 96 reactions
optimal Nucleofector® Kit for a selection of cell lines
are already available and can be downloaded from
www.lonza.com/celldatabase. Lonza’s R&D Team is Cell Line 96-well Nucleofector® Kit SF
continuously optimizing the Nucleofection® Protocols
for new cell lines. Make sure you are up-to-date with
our latest development by visiting our cell database.
– High throughput transfection with excellent Cell Line 96-well Nucleofector® Kit SG
performance and high reliability VHCA-1003 96 reactions
– siRNA and shRNA screening in cell lines VHCA-2003 960 reactions
with high medical relevance
– High throughput protein expression in
suspension cell lines
185
Cell Line Optimization Nucleofector® Kit
Nucleofection® of
Cell Lines
The advanced Amaxa® Cell Line Optimization Ordering Information

Nucleofector® Kit is the ideal tool for the transfection of
Cat. No. Size Price
virtually any difficult-to-transfect cell line. It enables you
to conveniently determine the optimal Nucleofection® Cell Line Optimization Nucleofector® Kit
condition of your cell line of interest within one
VCO-1001 18 reactions
experiment. The kit has been significantly simplified and
now requires only 18 reactions instead of the former 30.
Moreover, the optimization strategy has been updated
using a series of new programs and Nucleofector® Solution
combinations. The kit contains two different Amaxa®
Cell Line Nucleofector® Solutions, V and L, which should
be tested with seven different Nucleofector® Programs.
Fine-tuning to achieve optimal results can then be
performed together with Lonza’s Scientific Support Team.
– Efficient transfection of virtually any
difficult-to-transfect cell line
– Simple and rapid optimization completed within just
one experiment
– Up-to-date optimization strategy – new program and
Amaxa® Cell Line Nucleofector® Solution combinations
1 Solution L V 2 Solution V
Program 1 A-020 A-020

Program 2 T- 020 T- 020 > > > +
Program 3 T -030 T -030

Program 4 X - 0 01 X - 0 01 3
> > >
Program 5 X-005 X-005

Program 6 L-029 L-029
Program 7 D - 023 D - 023
Step 1 Step 2 Step 3

The cell line of interest is transfected The Nucleofector® Solution and program A further fine tuning of the Nucleofection®
with the Nucleofector® Solutions L and which result in highest transfection conditions can be performed with the help
V in combination with seven different efficiencies with lowest mortality are of our Scientific Support Team.
Nucleofector® Programs. selected.
Related Products
Classical Media 251 – 260
186
Cell Line Optimization 96-well Nucleofector® Kit
Nucleofection® of
Cell Lines
With the unique capability of the 96-well Shuttle® System to Ordering Information
address up to 96 different programs in one Nucleocuvette®
Cat. No. Size Price
Plate, cell line optimizations are easily performed. The
Amaxa® Cell Line Optimization 96-well Nucleofector® Kit Cell Line Optimization 96-well Nucleofector® Kit
is the ideal tool to conveniently and rapidly determine
VHCO-1001 96 reactions
the optimal 96-well Nucleofection® Condition of virtually
any difficult-to-transfect cell line within one experiment.
31 different programs plus one control are tested
with the Cell Line 96-well Nucleofector® Solutions SE,
SF and SG in one 96-well Nucleocuvette® Plate. Once
the best Cell Line Nucleofector® Solution and program
have been determined, Lonza‘s Scientific Support Team
is available to assist you in improving the results even
further.
– Transfection of virtually any difficult-to-transfect
cell line
– Simple and rapid optimization within just one
experiment and plate
1 SE SF SG > > > 2
1 2 3 4 5 6 7 8 9 10 11 12
+
A
B 96-well Nucleofector ®
C Solution SF
D
3
> > >
E
F
G
H
Step 1 Step 2 Step 3

The cell line of interest is transfected with The 96-well Nucleofector® Solution and (optional) A further fine tuning of the
the 96-well Nucleofector® Solutions SE, SF program which result in highest transfection Nucleofection® Conditions can be performed
and SG in combination with 31 different efficiency with lowest mortality are selected. with the help of our Scientific Support Team.
Nucleofector® Programs plus a negative Optimal Nucleofection® Condition: dark pink
control. Control wells: encircled dark pink dot; suboptimal conditions: medium pink
dots. dots; inappropriate conditions: light pink dots.
Related Products
Classical Media 251 – 260
187
cGMP Cell Line Nucleofector® Kits
Nucleofector® Kit
Basic Parasite
The Amaxa® cGMP Cell Line Nucleofector® Solutions enable Ordering Information
you to perform your transfection experiments under
Cat. No. Size Price
animal component-free conditions. They are, therefore,
the ideal tool for the Nucleofection® of cell lines used to cGMP Cell Line Nucleofector® Kit C
generate stable protein-producing clones for large-scale
VGA-1004 25 reactions
protein production.
– Efficient transfection of cell lines relevant to protein cGMP Cell Line Nucleofector® Kit L
production, e.g., CHO, suspension CHO, suspension
293, NS0
– Non-animal-origin free formulation cGMP Cell Line Nucleofector® Kit R
– Production under cGMP conditions reduces
regulatory concerns
– Certified for absence of DNA/RNA, DNase/RNase, cGMP Cell Line Nucleofector® Kit T
particles and endotoxins
cGMP Cell Line Nucleofector® Kit V

cGMP Cell Line 96-well Nucleofector® Kits can be provided on request.
Basic Parasite Nucleofector® Kits
Parasitic protozoa infect vertebrates and invertebrates Ordering Information

and some are even parasitic in plants. In humans,
Cat. No. Size Price
they can cause severe diseases such as Malaria
(Plasmodium), Sleeping Sickness (Trypanosoma) or Basic Parasite Starter Nucleofector® Kit
Leishmaniasis (Leishmania). Nucleofection® has proven
to provide strong increased transfection efficiencies VMI-1001 10 reactions
(e.g., in Plasmodium berghei and Trypanosoma brucei) Basic Parasite Nucleofector® Kit 1
compared to standard methods such as electroporation
VMI-1011 25 reactions
or particle bombardment. Due to significant genotypic
and phenotypic diversity between species and life Basic Parasite Nucleofector® Kit 2
cycles, Lonza has developed two Amaxa® Basic Parasite
VMI-1021 25 reactions
Nucleofector® Kits (1 and 2) and an easy-to-use Amaxa®
Basic Parasite Nucleofector® Starter Kit.
Nucleofection® of the rodent malaria parasite Plasmodium berghei.

Plasmodium berghei parasites were transfected with a reporter vector
containing two genes encoding for Green Fluorescent Protein (GFP) and
Red Fluorescent Protein (RFP) under control of sex-specific promoters.
After selection of transgenic parasites, sexual cells (gametocytes) of these
parasites were analyzed by fluorescence microscopy. Male cells showed
green and female cells a red fluorescence.
(Data kindly provided by Chris Janse, Blandine Franke-Fayard and Andrew
Waters, Leiden Malaria Research Group, Department of Parasitology, Leiden
University Medical Centre, Netherlands.)
188
FAQs on Nucleofection®
Q. Why is the Amaxa® Nucleofector® Technology ideal this. It comes with different cuvettes designed for
for primary cells and difficult-to-transfect cell lines? 20 µl sample volume. With the Small Cell Number
A. There are several reasons to choose the Amaxa® Nucleofector® Kit 20,000-100,000 cells per sample
Nucleofector® Technology for your gene transfer are used.
experiments. One is the direct transfer of DNA For the 96-well Shuttle® System, the sample
into the cell nucleus. This makes gene expression volume is 20 µl (typically cutting the cell number
independent from cell division. Therefore, the recommended for the single cuvette by 1/5). The
technique is the ideal tool for primary cells, even lower limits are often dictated by the minimum
for non-dividing cells, such as neurons. In cell amount of cells required for optimal plating density
lines as well as primary cells, this yields gene on 96-well cell culture plates.
expression shortly after transfection and in many Q. When transfecting cancer cell lines, do I need to be
cases transfection efficiencies of over 50% can be concerned about passage number?
detected in as little as 2-4 hours. A. For the most efficient gene transfer, we recommend
Q. What optimization is necessary to get the technology using cells that are in logarithmic growth phase
to work? and at a passage number of 10-15 (from the time of
A. In most cases none. For each cell type in our pro- thaw). This is because some cell lines differentiate
duct list, we offer a cell-type-specific solution and and change their features after many passages.
thoroughly optimized electrical parameters. These Q. What is the optimal size of DNA that you recommend
are already pre-programmed in the Nucleofector® for Nucleofection®?
Device. Our Amaxa® Optimized Protocols give you A. We routinely use plasmids of 4-7 kb in our
recommendations regarding cell culture and laboratories and plasmids up to approximately
handling for optimum transfection results. 20 kb should not be a problem. The optimized
Q. What can I do when my cell of interest is not in your conditions are cell-type-specific. That means you
range of products? can use the identical set up for DNA, siRNA, mRNA,
A. Lonza is constantly developing new Amaxa® peptide and large molecules like bacterial artificial
Nucleofector® Kits and Optimized Protocols for an chromosomes (BACs) as well.
increasing number of primary cells and cell lines. It is very likely that you will see a decrease in the
In order to obtain the latest information, check our transient efficiency when BACs are used as a
website or contact our Scientific Support Team. substrate, but in general it works very well. You
If your target cell is a cell line, we offer the Cell Line also have to consider that a) by using 5-10 µg
Optimization Nucleofector® Kit. This kit enables DNA you have less copies in your experiment than
a rapid and simple determination of the optimal with standard constructs which leads to lower
Nucleofection® Conditions for your cell line of interest, brightness of the cells, and b) the quality of the
including those which are difficult-to-transfect. DNA is very important. So try to use endotoxin free
Q. What are your recommendations for minimum and DNA preparations, and check the DNA quality on an
maximum cell numbers for Nucleofection®? agarose gel to be sure that the DNA isn’t nicked.
A. The recommended cell number will vary depending
on which Amaxa® Optimized Protocol is being used.
In general for the Nucleofector® Device (single
cuvette format), using less than 2x105 cells per
reaction causes a major increase in cell mortality.
For some cell lines, we have tried cell numbers up
to 3x107. The problem here is that you end up with
a very large cell pellet leading to a dilution of the
Nucleofector® Solution as the maximum cuvette
volume is 100 µl to 110 µl. We suggest increasing
the cell number successively from experiment to
experiment but not exceeding a cell pellet volume
of 50 µl. Our Small Cell Number Nucleofector® Kit
for primary neurons represents an exception to
189
Q. How do you determine cell death? By comparing the X-values for the untreated sample
A. We determine cell death in two ways: and the transfected samples you can calculate how
1) ToxiLight® Non-Destructive Cytotoxicity many dead cells you have in your treated samples.
BioAssay Kit. ToxiLight® is a patented luminescent Q. Does your system work for co-transfections of
assay that measures the release of adenylate plasmids?
kinase. Unequivocal results are available in less A. In principle, co-transfection of different plasmids
than 10 minutes. It can detect as few as 10 dead should not be a problem. However, the total amount
cells per well without requiring a lysis step. Please of DNA used is a crucial point because high amounts
see more details on page 226. of DNA could cause decreased cell viability. For the
2) Flow cytometry determination of viable/dead Nucleofector® Device (single cuvette format), we
cells by propidium iodide staining. We normally recommend starting co-transfection experiments
analyze transfection efficiency in living cells by flow with 1 µg - 5 µg total DNA first and then successively
cytometry: increasing the DNA amount if necessary.
We first exclude cellular debris by gating for the Also verify that both plasmids will be expressed in
"normal" population, (regarding size and granularity) this cell type. For the 96-well Shuttle® System you
in the forward-sidescatter. From this gated should calculate 1/5 of the amount; 0.2 µg - 1 µg
population we determine dying cells by propidium DNA per vector.
iodide staining and exclude them from analysis by Q Can I use the Amaxa® Nucleofector® Technology for
setting another gate. So, only those cells which are RNAi applications? How do I start?
in the FSC-SSC gate and are not propidium iodide A. Yes. The Amaxa® Nucleofector® Technology can
positive are analyzed for efficiency. be easily applied for any RNAi substrate (siRNA,
To be even more precise in the determination of shRNA, miRNA). You can use the same conditions
the mortality for adherent cells, we also collect the described in the cell-type-specific optimized
detached cells in the supernatant and combine these protocol for DNA vectors (cDNA, shRNA or miRNA
after trypsinization with the former adherent cells expressing plasmids) or oligonucleotides (siRNA,
and include them in the flow cytometry analysis. miRNA inhibtors).
This helps ensure that our data is complete and Prior to beginning comprehensive experiments,
accurate. multiple parameters associated with experimental
3) Flow cytometry determination of total cell loss design need to be optimized:
induced by Nucleofection® (optional method): Selection of appropriate controls (non-targeting
In order to get an idea about the total cell loss, we siRNA, siRNA targeting a house-keeping gene,
compare the initial cell number per sample with the untreated cells).
final cell number per sample. Determination of minimum effective siRNA amount
For that we use APC-labeled beads from BD for your target gene in your cell type of interest.
Biosciences (to detect APC your flow cytometry Determination of the optimal analysis time point
needs a 4 channel laser, because APC is detected on (kinetics of down-regulation depend on target gene,
channel 4). cell type and analysis level).
We prepare a stock of beads (1000 beads/µl in Identification of a suitable and robust analysis
PBS). After 5 minutes of vortexing, we add 50 µl of method (i.e. mRNA, protein or phenotypic analysis).
the beads to the sample we want to analyze by flow Q. Do you have any helpful calculations or conversion
cytometry. information for siRNA?
To count the beads by flow cytometry you have A. On our website, we provide a siRNA calculator
to lower the threshold in the FSC/SSC down to 20 which allows you to calculate from either amount to
because they are much smaller than cells. concentration or from concentration to amount and
After analysis, we do the following calculation to from either molar amount to weight or from weight
define the number of used living cells in the analyzed to molar amount.
sample (input X, unknown value):
input X = number of used beads*/counted beads x
counted cells in R2**
* input: 50 µl = 50000 beads
** R2 gate means: normal cell population in FSC/SSC without cellular
debris (gate R1) AND without PI-postive cells in FL-2/FL-3
190
HiFect® Transfection Reagent
Transfection Reagent
HiFect®
The HiFect® Transfection Reagent is Lonza’s stand alone Ordering Information
biochemical transfection reagent for standard cell lines.
Cat. No. Size Price
While the well established Nucleofector® Technology
remains the best system for gene transfer into notoriously HiFect® Reagent
difficult-to-transfect cell lines and primary cells, the
VBA-1001 1 x 825 µl
HiFect® Transfection Reagent is the cost-effective
VBA-4001 4 x 825 µl
alternative for standard cell lines. As a company with
great expertise in transfection technology, Lonza prides
itself on having the ideal solution for any transfection
challenge.
– Superior transfection efficiencies and cell viabilities
– Cost effective
– Suitable for DNA and siRNA transfections
– Suitable for high throughput applications
– Scientific support by our trusted and experienced team
High Performance
Most transfection reagents are optimized for either high
efficiency or high cell viability. With Lonza's HiFect® High Efficiency Combined with Low Cytotoxicity
Transfection Reagent you can address both features You are working on a wide range of standard cell lines
equally. The data clearly show that using HiFect® and would like to have optimized transfection conditions
Reagent enables you to achieve superior efficiencies for all of them. With HiFect® Reagent – the transfection
and high viabilities in parallel, while cells maintain their reagent optimized by Lonza – you no longer have to
morphological and functional properties. keep several reagents in stock. Compared to other leading
transfection reagents, HiFect® Reagent ensures excellent
efficiencies and the lowest mortality rates.
A B
100 HiFect™
Competitor reagent L
Competitor reagent F
90
80
70
60
C 50
40
30
20
10
0 CHO-K1 [ATCC® CCL-61™] COS-7 MCF7 [ATCC® HTB-22™]

Cells maintain their normal morphology. 293 cells were transfected with HiFect® Transfection Reagent provides high efficiencies. Different cells
the pmaxGFP® Vector (A) or a plasmid containing the cDNA sequence for a have been transfected with the pmaxGFP® Vector using either HiFect® Reagent
plasma membrane receptor-YFP fusion protein (B) using HiFect® Reagent. or other often used transfection reagents (competitor reagent L, competitor
Cells were stained with propidium iodide to visualize cell nuclei (A; red). reagent F). Efficiencies were measured by flow cytometry 24 hours post-
maxGFP® Reporter Protein was located in the cytosol of transfected cells transfection.
(A). YFP fusion protein was correctly targeted to the cell membrane (B). The
overall transfection efficiency was 80%. HepG2 cells were transfected with a
YFP expression plasmid using HiFect® Reagent (C). The overall transfection
efficiency was 40%.
(Data courtesy of V. Keitel, F. Schliess and D. Häussinger, Clinic for
Gastroenterology, Hepatology and Infectiology, Heinrich-Heine-University
Düsseldorf, Germany.)
191
pmaxFP® Fluorescent Protein Expression Vectors
Vectors
Lonza offers pmaxFP® Vectors expressing green, yellow and red fluorescent proteins.
– Monitor gene expression and protein localization in living mammalian cells
– Generate green, yellow or red fluorescent fusion proteins
– Study gene-regulation by using promoter-less (PRL) versions
Your benefits
■ Easy analysis
– Very bright green, yellow or red fluorescence
■ Non-toxic
– Suitable for transient and stable expression
■ Affordable
– Affordable pricing and license-free use for research purposes for for-profit entities
during the first six months after purchase
■ Flexible
– Various fusion constructs available; compatible with Nucleofector® Technology
A B C
HUVECs were transfected by Nucleofection® with 2 µg pmaxFP®-Green-C (A), pmaxFP®-Yellow-C (B) or pmaxFP®-Red-C
Vectors (C) and analyzed by fluorescence microscopy after 24 hours.
Propagation of pmaxFP® Vectors in E. coli

Suitable host strains DH5α HB101, and other general purpose strains; single-
stranded DNA production requires a host containing an
F plasmid such as JM109 or XL1-Blue; for production of
non-methylated DNA JM110 or SCS110 is recommended.
Selectable marker Plasmid confers resistance to kanamycin (30 µg/ml)
to E. coli hosts
E. coli replication origin pUC
Copy number ~500
Plasmid incompatibility pMB1/ColE1
group
NOTE: pmaxFP® Vector products contain a proprietary nucleic acid coding for a proprietary fluorescent protein(s)
intended to be used for research purposes only. Any use of proprietary nucleic acid or fluorescent proteins coding by
proprietary nucleic acid other than for research use is strictly prohibited. USE IN ANY OTHER APPLICATION REQUIRES
LICENSE FROM EVROGEN. To obtain such a license, please contact Evrogen at license@evrogen.com.
192
pmaxFP® Vector Backbone Information
Vectors
pmaxFP® Vectors for C-terminal Fusions
Immediate early promoter of cytomegalovirus (PCMV IE) for protein expression
SV40 origin for replication in mammalian cells
pUC origin of replication for propagation in E. coli
f1 origin for single-stranded DNA production
SV40 early promoter provides neomycin resistance gene expression to select stably transfected
eukaryotic cells using G418
Bacterial promoter (P) provides kanamycin resistance gene expression in E. coli

To increase maxFP®-Green, -Yellow or -Red mRNA translation efficiency, Kozak consensus translation initiation
site is generated upstream of maxFP®-Green, -Yellow or -Red Gene coding sequence
Multiple cloning site (MCS) is located between maxFP®-Green, -Yellow or -Red Gene coding sequence and SV40
polyadenylation signal (SV40 poly A)
pmaxFP® Vectors for N-terminal Fusions

Immediate early promoter of cytomegalovirus (PCMV IE) for protein expression
SV40 early promoter provides neomycin resistance gene expression to select stably transfected eukaryotic
cells using G418

To increase maxFP®-Green, -Yellow or -Red Gene mRNA translation efficiency, Kozak consensus translation initiation
Multiple cloning site (MCS) is located between PCMV IE and maxFP®-Green, -Yellow or -Red Gene coding sequence
Promoterless (PRL) pmaxFP® Vectors

SV40 early promoter provides neomycin resistance gene expression to select stably transfected eukaryotic cells
using G418

To increase maxFP®-Green, -Yellow or -Red Gene mRNA translation efficiency, Kozak consensus translation initiation
Multiple cloning site (MCS) is located upstream of maxFP®-Green, -Yellow or -Red Gene coding sequence
193
pmaxFP®-Green Vectors
Vectors
pmaxFP®-Green Vectors are eukaryotic (mammalian) maxFP®-Green Gene with humanized codon usage, i.e., it is
expression vectors encoding green fluorescent protein optimized for high expression in mammalian cells.
maxFP®-Green, an improved maxGFP® Reporter Protein – Extra fast protein maturation allowing early signal
variant from copepod Pontellina plumata. Different detection
from maxGFP® Reporter Protein, which is expressed by
our positive control pmaxGFP® Vector (provided in all – Bright green fluorescence, easy-to-detect
Nucleofector® Kits), maxFP®-Green Reporter Protein is – No aggregation, good performance in fusions
suitable for stable expression and generation of fusion – Suitable for stable expression
proteins. pmaxFP®-Green Vectors carry a synthetic
maxFP®-Green Reporter Protein Properties

Molecular weight 26 kDa Brightness1 37.1
Quantum yield 0.53 Excitation max 482 nm
Polypeptide length 232 AA pKa 5.2
Extinction 70,000 M-1 cm-1 Emission max 502 nm
Fluorescence green Structure monomer
1 Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
pmaxFP®-Green-C Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Green-C Vector
VDF-1011 20 µg
*not unique site.
sites are blocked by methylation. If you
Concentration: 0.5 µg/µl
wish to digest the vector using these sites,
you will need to transform the vector into a
dam- host and make fresh DNA.
Use:
– Generation of fusions to the C-terminus of the maxFP®-Green Reporter
Protein for expression and localization studies
– Expression of maxFP®-Green Reporter Protein in mammalian cells
pmaxFP®-Green-N Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Green-N Vector
VDF-1012 20 µg
*not unique site. Use:

– Generation of fusions to the N-terminus of maxFP®-Green Reporter
Protein for expression and localization studies
– Expression of maxFP®-Green Reporter Protein in mammalian cells
pmaxFP®-Green-PRL Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Green-PRL Vector
VDF-1013 20 µg

– Promoter studies in mammalian cells
194
pmaxFP®-Yellow Vectors
Vectors
pmaxFP®-Yellow Vectors are eukaryotic (mammalian) generation of fusion to its C-terminus was produced
expression vectors encoding fluorescent maxFP®-Yellow by random mutagenesis of maxFP®-Yellow Reporter
or maxFP®-Yellow-m Reporter Proteins, respectively. Protein. pmaxFP®-Yellow Vectors carry maxFP®-Yellow and
maxFP®-Yellow Reporter Protein is a mutant of a natural maxFP®-Yellow-m Genes with humanized codon usage.
yellow fluorescent protein from jellyfish Phialidium sp. – No aggregation, good performance in fusions
However, maxFP®-Yellow Reporter Protein was found to
be inappropriate for generating fusions to its C-terminus – maxFP®-Yellow-m: for C-terminal fusions
due to folding problems. To overcome this problem, – maxFP®-Yellow: for N-terminal fusions
maxFP®-Yellow-m Reporter Protein mutant suitable for
maxFP®-yellow Reporter Protein Properties maxFP®-yellow-m Reporter Protein Properties

Molecular weight 26 kDa Brightness1 52.0 Molecular weight 26 kDa Brightness1 48.4
Quantum yield 0.40 Excitation max 525 nm Quantum yield 0.39 Excitation max 525 nm
Polypeptide length 234 AA pKa 6.0 Polypeptide length 234 AA pKa 6.0
Extinction 130,000 M-1 cm-1 Emission max 537 nm Extinction 124,000 M-1 cm-1 Emission max 537 nm
Fluorescence yellow Structure monomer Fluorescence yellow Structure weak dimmer
1 Brightness is a product of extinction coefficient and quantum yield, divided by 1000. 1 Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
pmaxFP®-Yellow-C Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Yellow-C Vector
VDF-1021 20 µg
*not unique site.
# sites are blocked by methylation. If you
you will need to transform the vector into a
Use:
– Expression of maxFP®-Yellow-m Reporter Protein in mammalian cells
– Generation of fusions to the C-terminus of maxFP®-Yellow-m Reporter
Protein for expression, localization and cellular dynamic studies
pmaxFP®-Yellow-N Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Yellow-N Vector
VDF-1022 20 µg

– Expression of maxFP®-Yellow Reporter Protein in mammalian cells
– Generation of fusions to the N-terminus of maxFP®-Yellow Reporter
Protein for expression, localization and cellular dynamic studies
pmaxFP®-Yellow-PRL Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Yellow-PRL Vector
VDF-1023 20 µg

195
pmaxFP®-Red Vectors
Vectors
pmaxFP®-Red Vectors are eukaryotic (mammalian) – True red fluorescence (no interference with green or
expression vectors carrying maxFP®-Red Gene with yellow fluorescence)
humanized codon usage. maxFP®-Red Reporter Protein is – No aggregation, good performance in fusions
a novel red fluorescent protein obtained by mutagenesis
of an Anthomedusae jellyfish chromoprotein. – Suitable for stable expression
maxFP®-Red Reporter Protein Properties

Molecular weight 27 kDa Brightness1 8.8
Quantum yield 0.20 Excitation max 584 nm
Polypeptide length 242 AA pKa 5.0
Extinction 44,000 M-1 cm-1 Emission max 610 nm
Fluorescence red Structure monomer
1 Brightness is a product of extinction coefficient and quantum yield, divided by 1000.
pmaxFP®-Red-C Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Red-C Vector
VDF-1031 20 µg
# sites are blocked by methylation. If you
you will need to transform the vector into a Use:
– Expression of maxFP®-Red Reporter Protein in mammalian cells
– Generation of fusions to the C-terminus of maxFP®-Red Reporter Protein
for expression and localization studies, protein-protein interaction and
co-localization studies
pmaxFP®-Red-N Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Red-N Vector
VDF-1032 20 µg
Use:
– Expression of maxFP®-Red Reporter Protein in mammalian cells
– Generation of fusions to the N-terminus of maxFP®-Red Reporter Protein
for expression and localization studies, protein-protein interaction and
co-localization studies
pmaxFP®-Red-PRL Vector Ordering Information

Cat. No. Size Price
pmaxFP®-Red-PRL Vector
VDF-1033 20 µg
Use:
196
pmaxCloning™ Vector
Vectors
– High expression rates in mammalian cells Ordering Information
– License-free use for research purposes Cat. No. Size Price
– Multiple cloning site for convenient insertion of the
gene-of-interest
VDC-1040 20 µg
NOTE: The CMV promoter is covered under the U.S. patents 5,168,062 and
5,385,839 and its use is permitted for research purposes only. Any other
use of the CMV promoter requires a license from the University of Iowa
Research Foundation, 214 Technology Innovation Center, Iowa City, IA.
197
MycoZap™ Mycoplasma Elimination Reagent
MycoZap™
Reports are that 5 - 35% of all cells in continuous culture are Ordering Information
contaminated with mycoplasma. A cell line infection can Cat. No. Size Price
affect every known process in the cell and can seriously
impact the reliability, reproducibility and consistency MycoZap™ Mycoplasma Elimination Reagent
of results. Additionally, mycoplasma infections can LT07-818 1 treatment
spread easily within the culture environment. To monitor LT07-918 5 treatments
for such infections, regular testing of cell lines should

be performed using Lonza’s MycoAlert® Mycoplasma Competitor 1 –
Detection Kit. Where contamination has occurred, and the Effect on1 —cell
Competitor viability
Effect andandmycoplasma
on cell viability removal
mycoplasma removal
sample absolutely cannot be discarded, the MycoZap™ 100 10000000
ViaLight® Plus RLUs

MycoAlert® ratio
Mycoplasma Elimination Reagent has been optimized to Mycoplasma detected
1000000
eliminate mycoplasma with minimal toxic effects on the 10

100000
cells. 10000
The MycoZap™ Reagent eliminates mycoplasma by using 1

1000
a combination of antibiotic and antimetabolic agents. No mycoplasma detected

100
This approach allows for a highly reliable and definite 10
elimination of mycoplasma that cannot be achieved by

0.1 Day 0 Day 2 Day 4 Day 7 Day 9 Day 11 Day 16 1
untreated treated untreated viability treated viability

the use of antibiotics alone.
Cultures should be tested with the MycoAlert® Mycoplasma
Detection Kit at regular intervals for 4-6 weeks after Competitor 2 –
mycoplasma elimination to ensure fresh infections do Effect on
Competitor 2 —cell
Effectviability andandmycoplasma
mycoplasma removal
100 10000000
not arise. MycoZap™ Mycoplasma Elimination Reagent is:
MycoAlert® ratio

Mycoplasma detected
1000000
— Easy – simply add the reagent to your culture media 100000

10
— Universal – MycoZap™ Reagent can be used to 10000
eradicate Mollicutes, including mycoplasma, 1000

1
acholeplasma, spiroplasma and entomoplasma No mycoplasma detected
100
species in cell cultures 10

— Thorough – the combination of antibiotic and untreated treated untreated viability treated viability
antimetabolic agents ensure total removal of the
contamination
— Complete – the kit contains all the required reagents MycoZap™ Reagent –
Effect
MycoZap™on cell on
— Effect viability and
cell viability and mycoplasma removal
mycoplasma removal
■ Applications 100 10000000 ViaLight® Plus RLUs
MycoAlert® ratio
– All cell culture

Mycoplasma detected
1000000
– Mycoplasma elimination 10
100000
10000
■ Storage Conditions 1000
2°C – 8°C 1
100
No mycoplasma detected
10
0.1 Day 0 Day 4 Day 8 Day 11 Day17 1
– Both competitors' products take at least 2 weeks to eliminate the

mycoplasma infection
– Competitor 2’s treatment adversely affects cell viability
– The MycoZap™ Reagent treatment eliminates mycoplasma in as few as
4 days
Related Products – The MycoZap™ Reagent treatment kills mycoplasma with minimal impact
on cell viability
MycoAlert® Mycoplasma Detection Kit 220
198
Antibiotics and Antimycotics
and Antimycotics
Antibiotics
Amphotericin B Penicillin-Streptomycin Mixture

contains 250 µg/ml amphotericin B contains 5,000 units potassium penicillin and 5,000 µg streptomycin
Storage Conditions: -10°C – -20°C sulfate per ml in 0.85% saline
Storage Conditions: -10°C – -20°C
17-836R 20 ml
17-603E 100 ml
17-836E 100 ml
Gentamicin Sulfate 50 mg/ml Penicillin-Streptomycin Mixture

contains 10,000 units potassium penicillin and 10,000 µg streptomycin
screw cap vial sulfate per ml in 0.85% saline
Storage Conditions: 15°C – 30°C Storage Conditions: -10°C – -20°C
17-518Z 1 × 10 ml 17-602E 100 ml
17-518L 10 × 10 ml 17-602F 500 ml

crimp top vial contains 20,000 units potassium penicillin and 20,000 µg streptomycin
Storage Conditions: 15°C – 30°C sulfate per ml in 0.85% saline
17-528Z 1 × 10 ml
09-757F 500 ml
Gentamicin Sulfate 10 mg/ml
screw cap vial Penicillin-Streptomycin Mixture
Storage Conditions: 15°C – 30°C contains 25,000 units potassium penicillin and 25,000 µg streptomycin
sulfate per ml in 0.85% saline
17-519Z 1 × 10 ml Storage Conditions: -10°C – -20°C
17-519L 10 × 10 ml
17-719R 25 × 4.5 ml
Gentamicin Sulfate
10 mg/ml Penicillin-Streptomycin-Amphotericin B Mixture
Storage Conditions: 15°C – 30°C contains 10,000 units potassium penicillin, 10,000 µg streptomycin sulfate
and 25 µg Amphotericin B per ml in 0.85% saline
BE02-012E 100 ml Storage Conditions: -10°C – -20°C
17-745H 20 ml
17-745E 100 ml
Penicillin-Streptomycin-L-glutamine Mixture
contains 25,000 units potassium penicillin, 25,000 µg streptomycin sulfate,
and 29.2 mg L-glutamine per ml
17-718R 25 × 4.5 ml
199
siRNA Test Kit
siRNA Test Kit
Establish your siRNA experiments faster than ever before. Ordering Information
The siRNA Test Kit is based on co-transfection of the
Cat. No. Size Price
pmaxGFP® Vector and an siRNA duplex directed against
maxGFP® Reporter Protein. Knock-down of maxGFP® siRNA Test Kit
Reporter Protein expression can be easily detected
VSC-1001 25 - 50 reactions
by fluorescence microscopy. The kit has already been
evaluated in multiple cell types such as Jurkat, THP-1,
U-937, NIH/3T3, or primary NHDF cells. It is not suitable for
use in primary human blood cells.
– Easy set-up of siRNA transfections
– Suitable for cell lines and primary adherent cells
0.5 μg 1 μg 2 μg
A B C
D E F
Example for Nucleofection® of NIH/3T3 cells with pmaxGFP® Vector and

siRNA. NIH/3T3 cells (ATCC® CRL-1658™) were transfected by Nucleofection®
with 0.5, 1 or 2 µg of the pmaxGFP® Vector only (A, B, C top row) or co-trans-
fected with 0.5, 1 or 2 µg the pmaxGFP® Vector and 1.5 µg siRNA directed
against maxGFP® Reporter Protein (D, E, F bottom row). Gene silencing
of maxGFP® Reporter Protein expression was monitored by fluorescence
microscopy, 24 hours post Nucleofection®.
siRNA technology licensed to QIAGEN is covered by various patent

applications, owned by the Massachusetts Institute of Technology,
Cambridge, MA, USA and others.
200
Peptide Transfection Control
Transfection Control
Nucleofection® for Peptide Transfection
Peptide
Start Nucleofection® of peptides quickly and easily. The Ordering Information
Amaxa® Peptide Transfection Control offers a simple
Cat. No. Size Price
method to control the peptide transfection process
when used together with your regular cell-type specific Peptide Transfection Control
Nucleofector® or 96-well Shuttle® Kits. The fluorescently
VFP-1001 50 µg (lyophilized)
labeled (FAM) control peptide is visible in the cytoplasm up
to several hours following transfection and shows that the
Nucleofector® Program enabled delivery of extracellular
cargos, such as your experimental peptides.
– Easily control peptide transfection optimizations
– Explore peptide function with direct delivery into the
cytoplasm
– Analyze active protein pathways with the same
program as for DNA and RNA
Nucleofection® of Amaxa® Peptide Transfection Control
Bright field and fluorescence images of SH-SY5Y cells 2 hours after Nucleofection® with the Amaxa® Peptide Transfection Control
at the given concentrations. Cells show ‘normal’ morphology and intracellular distribution of the fluorescently labeled (FAM)
peptideis homogenous.
(Image courtesy of Prof. R.Brock, Dept. of Biochemistry, Nijmegen Centre for Molecular Life Sciences, Netherlands.)
201
Transport™ Protein Delivery Reagent
Superior delivery of protein into live cells
Transport™ Protein Delivery Reagent is a revolutionary Ordering Information

new tool for non-cytotoxic delivery of biologically active
Cat. No. Size Price
cargo molecules (proteins, peptides, or small molecules)
Transport™ Protein Delivery Reagent
directly into live cells. Transport™ Reagent allows you to
50568 400 µg
quickly and easily study cell and protein function without
the problems of DNA transfections. This newly discovered
Cells Successfully Transfected with Transport™ Reagent.
peptide (patent pending) translocates itself and attaches
cargo molecules into cultured cells when added to the Cell Type
culture medium. Transport™ Reagent-cargo complex delivery Human Bronchial Epithelial Cells
is very efficient (up to 100% of cultured primary cells or Human Epidermal Keratinocytes
cell lines). Human Aortic Smooth Muscle Cells
Human Umbilical Vein Endothelial Cells
The conjugation of Transport™ Reagent and a cargo Human Dermal Fibroblasts
molecule occurs through the 2-pyridyldithio group on Human Neural Progenitors
the reagent that forms a disulfide bond with any free NIH3T3 Cells
thiol (cysteine) situated on the cargo molecule. Coupling 293 Cells
of Transport™ Reagent with cargo molecules is a simple C2C12 Cells
process – simply incubate appropriate amounts of each CHO Cells
component at room temperature for 15 – 30 minutes,
Rat Hepatocytes
dilute with culture medium and apply to cells in culture.
Rat Hippocampal/Cortical Neurons
400 µg of Transport™ Protein Delivery Reagent is sufficient
for 40 – 80 reactions in 6-well plates or 1,000 96-well AoSM Cells
transfections.
— Fast – Deliver macromolecules into cells in less than
1 – 2 hours. Assay for activity immediately
— Efficient – Deliver biologically active proteins, peptides,
and other macromolecules in up to 100% of cells
— Flexible – Transfect a wide variety of cell types including Transport™ Reagent Company A
difficult-to-transfect primary cells NIH3T3 Cells
— Non-cytotoxic – Assay live cells
■ Storage Conditions
-20°C
Protein Delivery Transport™ Reagent Company B

4 Transport™ Reagent and protein delivery reagents from Company A and
Activity (OD)
TransportTM Reagent
Company A Company B were used to transfect cells with β-Galactosidase. Note higher
3.5
activity of cells transfected with Transport™ Reagent and absence of
precipitation.
3
2.5
■ Simple Protocol
1.5
Combine Transport™ Reagent with your
1 molecule, incubate at room temperature
for 15 – 30 minutes, then add to your
0.5 cells.
0 NIH3T3 HUVEC NHBE AoSMC
Cell Type
Transport™ Reagent and another commercial protein delivery reagent

(Company A) were compared for delivering β-Galactosidase into a variety of
cell types. Activity post transfection was measured.
202
PrimeFect™ DNA and siRNA Transfection Reagents
Optimized for delivery of DNA and siRNA into primary cells
PrimeFect™ I and II DNA Transfection Reagents, for plasmid Ordering Information

DNA delivery, and PrimeFect™ siRNA Transfection Reagent
Cat. No. Size Price
for siRNA delivery, offer the advantages of proven high
PrimeFect™ I DNA Transfection Reagent
transfection efficiency and low cytotoxicity when used with
PA-3267 1 ml
Clonetics® Primary Human Cells and Media. (Table below)
— Simple and fast protocol – Already optimized for use PrimeFect™ II DNA Transfection Reagent
with Clonetics® Primary Human Cells PA-3268 0.5 ml
— High transfection efficiency – Achieve expression in a PrimeFect™ siRNA Transfection Reagent

large population of cells for experimental success PA-3269 0.5 ml
— Low cytotoxicity – Maintain cell density and obtain a PrimeFect™ Dilution Buffer
higher yield of transfections PA-3271 50 ml
— Transfection in the presence or absence of serum –
Does not require media changes and can be carried
out in serum containing media
2°C – 8°C, do not freeze
■ Applications
– Plasmid DNA delivery
– siRNA delivery
Recommended PrimeFect™ Transfection Reagents for A comparison of PrimeFect™ I Reagent with other leading
Clonetics® and Poietics® Primary Human Cells transfection reagents
80
% of Transfected Cells
Company I
Plasmid DNA siRNA Company R
Clonetics® or Poietics® 70
PrimeFectTM I
Primary Cell Type PrimeFect™ I PrimeFect™ II PrimeFect™ siRNA
60
Human Umbilical Vein
■ ■
Endothelial Cells – HUVEC 50
Human Blood
40
Microvascular Endothelial ■ ■
Cells – HMVEC-dBl 30
Human Mammary
■ ■ 20
Epithelial Cells – HMEC
Human Mesenchymal Stem 10
■ ■
Cells – HMSC
0 AoSMC SkMC HMVEC-dBI HMEC NHEK
Human Coronary Artery Primary Cell Type
■ ■
Endothelial Cells – HCAEC
Plasmid DNA was complexed with reagent and applied to 50 – 80%
Normal Human Epidermal
Keratinocytes – NHEK
■ ■ confluent monolayers in 6-well tissue culture dishes in accordance with
the manufacturer’s instructions. Cells were harvested 48 hours post-
Human Aortic Endothelial transfection and quantitated by flow cytometry.
■ ■
Cells – HAEC
Human Aortic Smooth AoSMC – Human Aortic Smooth Muscle Cells, SkMC – Human Skeletal Muscle
■ ■
Muscle Cells – AoSMC Cells, HMVEC-dBl – Human Blood Microvascular Endothelial Cells, HMEC
Human Skeletal Muscle – Human Mammary Epithelial Cells, NHEK – Normal Human Epidermal
Cells – SkMC
■ ■ Keratinocytes.
Normal Human Bronchial
■ ■
Epithelial Cells – NHBE
Human Prostate Epithelial
■ ■
Cells – PrEC
Related Products
Clonetics® Primary Cells and Media 2 –79
203
PrimeFect™ DNA and siRNA Transfection Reagents
Continued
Measurements of transfection efficiency using PrimeFect™ Transfection Reagents
PrimeFect™ I PrimeFect™ II PrimeFect™ siRNA
A reporter plasmid expressing green fluorescent protein DNA (1μg) was transfected into HMVEC-d Bl - Human Blood Microvascular Endothelial Cells using
PrimeFect™ I and HAEC - Human Aortic Endothelial Cells using PrimeFect™ II. Cells were photographed and analyzed by flow cytometry at 48 hours post-transfection.
Cy3-labelled siRNA (25 nM final conc.) was transfected into HMEC - Human Mammary Epithelial Cells using PrimeFect™ siRNA. Cells were photographed and analyzed
by flow cytometry at 24 hours post-transfection. (All images are 10X magnification).
NOTE: PrimeFect™ I: Use of this product is covered under patents and patents pending. This product is sold under license from Synvolux Therapeutics BV and its
use is limited solely to research purposes.
NOTE: PrimeFect™ II or PrimeFect™ siRNA: Use of this product is covered under patents and patents pending. This product is sold under license from Mirus Bio
Corporation and its use is limited solely to research purposes.
Retronectin® Recombinant Human Fibronectin Fragment
Recombinant human fibronectin fragment CH-296 Ordering Information

produced in E. coli. When coated on the surface of Cat. No. Size Price
flasks and plates, Retronectin® significantly enhances Retronectin® Recombinant Human Fibronectin Fragment
retrovirus-mediated gene transfer into mammalian cells. (Lyophilized)
T100A 0.5 mg
■ Storage Conditions T100B 2.5 mg
-20°C Retronectin® Dish

(Retronectin® pre-coated 35 mm dishes)
T110A 10 dishes (35 mm)
204
Transport™ Reagents FAQ's
Reagents FAQs
Transport™
Q. What type of studies would one do with Transport™
Protein Delivery Reagent?
A. Studies include understanding interactions of
proteins within the cell, using inhibitors to study
signal transduction, effects of transient over-
expression of proteins, and screening peptide
libraries.
Q. What cells have been transfected using Transport™
Reagent?
A. Several cell lines and primary cells have been
successfully transfected with Transport™ Reagent,
including:
– Human bronchial epithelial cells
– Human epidermal keratinocytes
– Human aortic smooth muscle cells
– HUVEC
– Human dermal fibroblasts
– Human neural progenitors
– NIH3T3
– 293
– C2C12
– CHO
– Rat hepatocytes
– Rat hippocampal/cortical neurons
Q. What happens to the Transport™ Reagent-Cargo
complex after it enters the cell?
A. After entering the cell, the disulfide bond
connecting Transport™ Reagent to the cargo
molecule is reduced due to the intracellular
environment and the complex dissociates.
205
Notes
206
Arrays
Chapter 3
StellARray™ Gene Expression Systems
Expression Systems
StellARray™ Gene
Array Products and Services

Introduction 209 Mouse Gene StellARray™ qPCR Arrays 214
GeneSieve™ Query 210 Alzheimer's Disease 214
StellARray™ qPCR Arrays 211 AMPK Signaling 214
Global Pattern Recognition™ Analysis Tool 210 Antigen Processing & Presentation 214
Human Gene StellARray™ qPCR Arrays 211 Apoptosis 214
Alzheimer's Desease 211 Autophagy 214
AMPK Signaling 211 B cell Receptor 214
Antigen Processing & Presentation 211 B cell Receptor and Signaling 214
Apoptosis 211 Blood Coagulation 214
Autophagy 211 Cell Cycle Tox and Cancer 214
B cell Receptor and Signaling 211 Cytokines and Receptors 214
Blood Coagulation 211 Diabesity 214
Cell Cycle Tox and Cancer 211 DNA Damage and Repair 214
Cytokines and Receptors 211 Electron Transport Toxicology 214
Diabesity 211 FoxP3 Target Genes 214
DNA Damage and Repair 212 General Toxicology 214
Electron Transport Toxicology 212 Growth and Development Tox 215
FoxP3 Target Genes 212 Hematopoetic Cell Lineage 215
General Toxicology 212 Huntingtons Disease 215
Growth and Development Tox 212 Immune Complement 215
Hematopoetic Cell Lineage 212 Immune System Phenotyping 215
Huntingtons Disease 212 Immunology 215
Immune Complement 212 Immunological Targets of NFKB 215
Immune System Phenotyping 212 Innate Immune Signaling 215
Immunology 212 Interferon Signaling & Response 215
Immunological Targets of NFKB 212 Lymphoma and Leukemia 215
Innate Immune Signaling 212 Mood Disorder 215
Interferon Signaling & Response 212 Natural Killer Cell 215
Lymphoma and Leukemia 212 Neurogeneration 215
Mood Disorder 212 NFKB Signaling 215
Neurogeneration 213 Notch Signaling 215
NFKB Signaling 213 Obesity 215
Notch Signaling 213 Osteoporosis 216
Obesity 213 p53 Signaling 216
Osteoporosis 213 Parkinson's 216
p53 Signaling 213 Pathogen Recognition 216
Parkinson's 213 Stem Cell 216
Stem Cell 213 Stress Response 216
Stress Response 213 T cell Receptor Signaling 216
T Cell Receptor Signaling 213 T helper 17 (Th 17) 216
T helper 17 (Th 17) 213 T Regulatory Phenotyping 216
T Regulatory Phenotyping 213 Targets of Wnt/β-catenin Signaling 216
Targets of Wnt/β-catenin Signaling 213 TGF-β Signaling 216
TGF-β Signaling 213 Toll-like Receptor Signaling 216
Toll-like Receptor Signaling 213 Wnt Signaling 216
Wnt Signaling 213
208
Expression Systems
StellARray™ Gene
A fundamental hinderance to the advancement of basic Arrays can come in the form of tissues from several donors
research and drug discovery is the availability of tools for a specific disease and the respective normal margin
that allow simple comparisons of “like” things in one tissue on one plate. It can take the form of oligonucleotide
experiment. Our challenge is to deliver arrays of hard- sequences relevant to a specific genome bound to a
to-source components that, when seen together in one solid support. In the case of StellARray™ qPCR Arrays,
experiment, deliver valuable context to the researcher. PCR primer sets of genes relevant to a specific disease or
cell state are offered on one 96-well or 384-well plate for
real-time PCR gene expression analysis.
We look forward to developing this new product offering

for you, as the need for basic research and drug discovery
mature and meaningful comparative experiments become
more complex.
209
Expression Systems
StellARray™ Gene
GeneSieve™ Query Single gene normalized fold change values for three
commonly used ‘normalizers’, sorted with respect to GPR
Challenging Dogma – Your cells are telling you more than ranking
you realize. 400
Fold Change
18s Normalizer
GAPDH Normalizer
B2M Normalizer
300
When using pre-defined normalizers, one often ignores

small changes in gene expression. The dogma is that 200
those small changes represent noise and are not relevant. 100
In actuality, small changes in expression of key genes can
significantly impact metabolic pathways. Consequently, 0
cell behavior is altered. Even housekeeping genes

change expression profiles, depending on the media or -100
environmental factors to which they are exposed.

-200
CD83
IGHA1
BLK
CD79A
LRMP
IL21R
IGHD
CD80
CD79B
FOSB
FOS
BCL11A
REL
LMO2
CD40
POU2AF1
PTPRC
TNRSF13C
EZH2
DTX1
CD19
LEF1
RAD51
IKZF1
IL8
CD86
IL2RA
TRAF4
PAX5
LTB
Fundamentally, researchers have operated under two
axioms when measuring gene or protein expression. Axiom 1. The top 30 (of 75) GPR-ranked genes are shown. Fold change values were
calculated using the ddCt method. The data was derived from the Lonza Human
Lymphoma and Leukemia 384 StellARray™ qPCR Array (Cat. No. 00188333) deter-
Axiom 1. You can predict a priori which gene is invariant mining the expression level changes in cDNA derived from Human Lymphoma
and thus useful as a normalizer. Yet, the expression levels compared to Normal Adjacent Tissue RNA (Applied Biosystems, Inc., Part No.
AM7268).
of all genes change at some time and there is normalizer Note: The varying profiles represent single gene normalizer bias. GPR ranks the
drift. So how can you select the best normalizer? statistical differences between sample groups AND THEN calculates a fold change
value. The ranking is NOT based on the magnitude of fold change.
A xiom 2. Only high fold change equals biological

significance, yet small message level changes may yield
important protein level information.
By using the logic of pre-selecting normalizers, your lab Global Pattern Recognition™ (GPR) fold change values
could miss significant expression changes that potentially 80
Statistically Ranked Fold Change
GPR Analysis
represent significant variations in cellular activity. 60
40
The simplest way to recognize relevant gene expression 20

changes is the StellARray™ Gene Expression System.
0
Follow these three steps from database search to -20
publishable results: -40
n GeneSieve™ Query -60
– Search a gene or biological pathway to screen 16 -80
million publications and find the most appropriate -100

CD83
IGHA1
BLK
CD79A
LRMP
IL21R
IGHD
CD80
CD79B
FOSB
FOS
BCL11A
REL
LMO2
CD40
POU2AF1
PTPRC
TNFRSF13C
EZH2
DTX1
CD19
LEF1
RAD51
IKZF1
IL8
CD86
ILZRA
TRAF4
PAX5
LTB
StellARray™ Product
– Return a list of biological pathways and related Axiom 2. The top 30 (of 75) GPR-ranked genes are shown. Fold change values were
StellARray™ qPCR Arrays calculated using the GPR multi-normalizer method. The data was derived from
the Lonza Human Lymphoma and Leukemia 384 StellARray™ qPCR Array (Cat. No.
– Type in a disease name or gene and see relevant 00188333) determining the expression level changes in cDNA derived from Human
Lymphoma compared to Normal Adjacent Tissue RNA (Applied Biosystems, Inc.,
arrays in seconds Part No. AM7268). The ranking via GPR reveals the true profile without normaliza-
tion-based or fold change-based bias.
Note: The GPR statistically ranked list reveals a different profile from the Normalizer/
Fold Change-biased profile. GPR reveals a profile with fold change values asymptoti-
cally approaching the cells’ real state.
210
Expression Systems
Continued
StellARray™ Gene
n StellARray™ qPCR Arrays n Global Pattern Recognition™ (GPR) Analysis Tool
– Primers arranged in 96-well and 384-well formats – Analyze data in minutes without housekeeping
for real-time PCR genes
– Determine gene expression levels or genomic DNA – Allows for quick and reliable analysis of real-tme PCR
copy number data
– Screen an entire biological pathway in one run – Returns a ranked gene list based on p-values
– Each 96-well StellARray™ qPCR Array contains – Normalizers determined by the experiment, not
94 gene-specific PCR primer pairs, plus genomic and a priori by a scientist
RNA controls
Human Gene StellARray™ qPCR Arrays

Cat. No. Plates Price Cat. No. Plates Price
Alzheimer’s Disease 96 StellARray™ qPCR Array B cell Receptor and Signaling 96 StellARray™ qPCR Array
00188211 2 x 96-well plates 00188215 2 x 96-well plates
00188257 2 x Fast 96-well plates 00188261 2 x Fast 96-well plates
Alzheimer’s Disease 384 StellARray™ qPCR Array B cell Receptor and Signaling 4 x 96 StellARray™ qPCR Array
Alzheimer’s Disease 4 x 96 StellARray™ qPCR Array
00188293 2 x 384-well plates
Blood Coagulation 96 StellARray™ qPCR Array
00188216 2 x 96-well plates
AMPK Signaling 96 StellARray™ qPCR Array 00188262 2 x Fast 96-well plates
00188658 2 x 96-well plates Blood Coagulation 4 x 96 StellARray™ qPCR Array
00188659 2 x Fast 96-well plates
00188298 2 x 384-well plates
AMPK Signaling 4 x 96 StellARray™ qPCR Array
00188657 2 x 384-well plates Cell Cycle Tox and Cancer 96 StellARray™ qPCR Array
Antigen Processing & Presentation 96 StellARray™
qPCR Array
Cell Cycle Tox and Cancer 4 x 96 StellARray™ qPCR Array
00188299 2 x 384-well plates
Antigen Processing & Presentation 4 x 96 StellARray™ Cytokines and Receptors 96 StellARray™ qPCR Array
qPCR Array 00188218 2 x 96-well plates
00188294 2 x 384-well plates 00188264 2 x Fast 96-well plates
Cytokines and Receptors 4 x 96 StellARray™ qPCR Array
Apoptosis 96 StellARray™ qPCR Array
00188300 2 x 384-well plates
00188259 2 x Fast 96-well plates Diabesity 384 StellARray™ qPCR Array
Apoptosis 4 x 96 StellARray™ qPCR Array 00188330 2 x 384-well plates
00188295 2 x 384-well plates
Autophagy 96 StellARray™ qPCR Array

Autophagy 4 x 96 StellARray™ qPCR Array
00188296 2 x 384-well plates
211
Expression Systems
Continued
StellARray™ Gene

DNA Damage and Repair 96 StellARray™ qPCR Array Immune Complement 96 StellARray™ qPCR Array
DNA Damage and Repair 4 x 96 StellARray™ qPCR Array Immune Complement 4 x 96 StellARray™ qPCR Array
Electron Transport Toxicology 96 StellARray™qPCR Array Immune System Phenotyping 96 StellARray™ qPCR Array
Electron Transport Toxicology 4 x 96 StellARray™ qPCR Array Immune System Phenotyping 4 x 96 StellARray™ qPCR Array
FoxP3 Target Genes 96 StellARray™ qPCR Array Immunology 384 StellARray™ qPCR Array
Immunological Targets of NFKB 96 StellARray™ qPCR Array
FoxP3 Target Genes 4 x 96 StellARray™ qPCR Array
00188303 2 x 384-well plates
General Toxicology 96 StellARray™ qPCR Array Immunological Targets of NFKB 4 x 96 StellARray™
00188232 2 x 96-well plates qPCR Array
00188268 2 x Fast 96-well plates 00188310 2 x 384-well plates
General Toxicology 384 StellARray™ qPCR Array Innate Immune Signaling 96 StellARray™ qPCR Array
00188331 2 x 384-well plates
General Toxicology 4 x 96 StellARray™ qPCR Array 00188275 2 x Fast 96-well plates
00188304 2 x 384-well plates
Innate Immune Signaling 4 x 96 StellARray™ qPCR Array
Growth and Development Tox 96 StellARray™ qPCR Array 00188311 2 x 384-well plates
00188233 2 x 96-well plates Interferon Signaling & Response 96 StellARray™ qPCR Array
Growth and Development Tox 4 x 96 StellARray™ qPCR Array 00188276 2 x Fast 96-well plates
00188305 2 x 384-well plates Interferon Signaling & Response 4 x 96 StellARray™
Hematopoetic Cell Lineage 96 StellARray™ qPCR Array qPCR Array
00188270 2 x Fast 96-well plates Lymphoma and Leukemia 384 StellARray™ qPCR Array
Hematopoetic Cell Lineage 4 x 96 StellARray™ qPCR Array 00188333 2 x 384-well plates
00188306 2 x 384-well plates
Mood Disorder 96 StellARray™ qPCR Array
Huntingtons Disease 96 StellARray™ qPCR Array 00188241 2 x 96-well plates
Mood Disorder 4 x 96 StellARray™ qPCR Array
Huntingtons Disease 4 x 96 StellARray™ qPCR Array 00188313 2 x 384-well plates
00188307 2 x 384-well plates
Mood Disorder 384 StellARray™ qPCR Array
00188334 2 x 384-well plates
212
Expression Systems
Continued
StellARray™ Gene

Neurodegeneration 96 StellARray™ qPCR Array Stress Response 4 x 96 StellARray™ qPCR Array
T cell Receptor Signaling 96 StellARray™ qPCR Array
Neurodegeneration 4 x 96 StellARray™ qPCR Array
00188314 2 x 384-well plates
NFKB Signaling 96 StellARray™ qPCR Array T cell Receptor Signaling 4 x 96 StellARray™ qPCR Array
T helper 17 (Th17) 96 StellARray™ qPCR Array
NFKB Signaling 4 x 96 StellARray™ qPCR Array
00188315 2 x 384-well plates
Notch Signaling 96 StellARray™ qPCR Array T helper 17 (Th17) 4 x 96 StellARray™ qPCR Array
T Regulatory Phenotyping 96 StellARray™ qPCR Array
Notch Signaling 4 x 96 StellARray™ qPCR Array 00188252 2 x 96-well plates
00188316 2 x 384-well plates
Obesity 96 StellARray™ qPCR Array T Regulatory Phenotyping 4 x 96 StellARray™ qPCR Array
00188281 2 x Fast 96-well plates Targets of Wnt/β-catenin Signaling 96 StellARray™
Obesity 4 x 96 StellARray™ qPCR Array qPCR Array
Osteoporosis 96 StellARray™ qPCR Array
Targets of Wnt/β-catenin Signaling 4 x 96 StellARray™
qPCR Array
00188325 2 x 384-well plates
Osteoporosis 4 x 96 StellARray™ qPCR Array
00188318 2 x 384-well plates TGF-β Signaling 96 StellARray™ qPCR Array
p53 Signaling 96 StellARray™ qPCR Array
00188283 2 x Fast 96-well plates TGF-β Signaling 4 x 96 StellARray™ qPCR Array
00188326 2 x 384-well plates
p53 Signaling 4 x 96 StellARray™ qPCR Array
00188319 2 x 384-well plates Toll-like Receptor Signaling 96 StellARray™ qPCR Array
Parkinson’s 96 StellARray™ qPCR Array
00188284 2 x Fast 96-well plates Toll-like Receptor Signaling 4 x 96 StellARray™ qPCR Array
Parkinson’s 4 x 96 StellARray™ qPCR Array
00188327 2 x 384-well plates
00188320 2 x 384-well plates
Wnt Signaling 96 StellARray™ qPCR Array
Stem Cell 384 StellARray™ qPCR Array
00188335 2 x 384-well plates
Stress Response 96 StellARray™ qPCR Array Wnt Signaling 4 x 96 StellARray™ qPCR Array
213
Expression Systems
Continued
StellARray™ Gene
Mouse Gene StellARray™ qPCR Arrays

Alzheimer’s Disease 96 StellARray™ qPCR Array Blood Coagulation 96 StellARray™ qPCR Array
Alzheimer’s Disease 384 StellARray™ qPCR Array Blood Coagulation 4 x 96 StellARray™ qPCR Array
00188199 2 x 384-well plates
00188430 2 x 384-well plates
Alzheimer’s Disease 4 x 96 StellARray™ qPCR Array
00188424 2 x 384-well plates
Cell Cycle Tox and Cancer 96 StellARray™ qPCR Array
AMPK Signaling 96 StellARray™ qPCR Array 00188372 2 x Fast 96-well plates
Cell Cycle Tox and Cancer 96 StellARray™ qPCR Array
00188431 2 x 384-well plates
AMPK Signaling 4 x 96 StellARray™ qPCR Array
Cytokines and Receptors 96 StellARray™ qPCR Array
00188660 2 x 384-well plates
Antigen Processing & Presentation 96 StellARray™
qPCR Array
Cytokines and Receptors 4 x 96 StellARray™ qPCR Array
00188432 2 x 384-well plates
Antigen Processing & Presentation 4 x 96 StellARray™ Diabesity 384 StellARray™ qPCR Array
00188425 2 x 384-well plates DNA Damage and Repair 96 StellARray™ qPCR Array
Apoptosis 96 StellARray™ qPCR Array 00188140 2 x 96-well plates
00188367 2 x Fast 96-well plates DNA Damage and Repair 4 x 96 StellARray™ qPCR Array
Apoptosis 4 x 96 StellARray™ qPCR Array 00188433 2 x 384-well plates
00188426 2 x 384-well plates Electron Transport Toxicology 96 StellARray™ qPCR Array
Autophagy 96 StellARray™ qPCR Array 00188161 2 x 96-well plates
00188368 2 x Fast 96-well plates Electron Transport Toxicology 4 x 96 StellARray™ qPCR Array
Autophagy 4 x 96 StellARray™ qPCR Array 00188434 2 x 384-well plates
00188427 2 x 384-well plates FoxP3 Target Genes 96 StellARray™ qPCR Array
B cell Developmental and Function 96 StellARray™ 00188162 2 x 96-well plates
qPCR Array 00188376 2 x Fast 96-well plates
00188076 2 x 96-well plates FoxP3 Target Genes 4 x 96 StellARray™ qPCR Array
B cell Developmental and Function 4 x 96 StellARray™ General Toxicology 96 StellARray™ qPCR Array
B cell Receptor and Signaling 96 StellARray™ qPCR Array General Toxicology 384 StellARray™ qPCR Array
00188370 2 x Fast 96-well plates General Toxicology 4 x 96 StellARray™ qPCR Array

B cell Receptor and Signaling 4 x 96 StellARray™ qPCR Array 00188436 2 x 384-well plates
00188429 2 x 384-well plates
214
Expression Systems
Continued
StellARray™ Gene

Growth and Development Tox 96 StellARray™ qPCR Array Interferon Signaling & Response 96 StellARray™ qPCR Array
Growth and Development Tox 4 x 96 StellARray™ qPCR Array Interferon Signaling & Response 4 x 96 StellARray™
00188437 2 x 384-well plates
qPCR Array
Hematopoetic Cell Lineage 96 StellARray™ qPCR Array
00188165 2 x 96-well plates Lymphoma and Leukemia 384 StellARray™ qPCR Array
Hematopoetic Cell Lineage 4 x 96 StellARray™ qPCR Array Mood Disorder 96 StellARray™ qPCR Array
Huntingtons Disease 96 StellARray™ qPCR Array
00188166 2 x 96-well plates Mood Disorder 4 x 96 StellARray™ qPCR Array
Huntingtons Disease 4 x 96 StellARray™ qPCR Array Mood Disorder 384 StellARray™ qPCR Array
Immune Complement 96 StellARray™ qPCR Array Natural Killer Cell 96 StellARray™ qPCR Array
Immune Complement 4 x 96 StellARray™ qPCR Array Natural Killer Cell 4 x 96 StellARray™ qPCR Array
Immune System Phenotyping 96 StellARray™ qPCR Array Neurodegeneration 96 StellARray™ qPCR Array
Immune System Phenotyping 4 x 96 StellARray™ qPCR Array Neurodegeneration 4 x 96 StellARray™ qPCR Array
Immunology 384 StellARray™ qPCR Array NFKB Signaling 96 StellARray™ qPCR Array
Immunological Targets of NFKB 96 StellARray™ qPCR Array
NFKB Signaling 4 x 96 StellARray™ qPCR Array
00188448 2 x 384-well plates
Immunological Targets of NFKB 4 x 96 StellARray™ qPCR Notch Signaling 96 StellARray™ qPCR Array
Array 00188184 2 x 96-well plates
Notch Signaling 4 x 96 StellARray™ qPCR Array
Innate Immune Signaling 96 StellARray™ qPCR Array
00188449 2 x 384-well plates
00188384 2 x Fast 96-well plates Obesity 96 StellARray™ qPCR Array
Innate Immune Signaling 4 x 96 StellARray™ qPCR Array 00188185 2 x 96-well plates
215
Expression Systems
Continued
StellARray™ Gene

Obesity 4 x 96 StellARray™ qPCR Array T Regulatory Phenotyping 96 StellARray™ qPCR Array

Osteoporosis 96 StellARray™ qPCR Array
T Regulatory Phenotyping 4 x 96 StellARray™ qPCR Array
00188458 2 x 384-well plates
Targets of Wnt/β-catenin Signaling 96 StellARray™
Osteoporosis 4 x 96 StellARray™ qPCR Array
qPCR Array
00188451 2 x 384-well plates
p53 Signaling 96 StellARray™ qPCR Array 00188400 2 x Fast 96-well plates
00188187 2 x 96-well plates Targets of Wnt/β-catenin Signaling 4 x 96 StellARray™
00188393 2 x Fast 96-well plates qPCR Array
p53 Signaling 4 x 96 StellARray™ qPCR Array 00188459 2 x 384-well plates
00188452 2 x 384-well plates TGF-β Signaling 96 StellARray™ qPCR Array
Parkinson’s 96 StellARray™ qPCR Array 00188195 2 x 96-well plates
00188394 2 x Fast 96-well plates TGF-β Signaling 4 x 96 StellARray™ qPCR Array
Parkinson’s 4 x 96 StellARray™ qPCR Array 00188460 2 x 384-well plates
00188453 2 x 384-well plates Toll-like Receptor Signaling 96 StellARray™ qPCR Array
Pathogen Recognition 96 StellARray™ qPCR Array 00188196 2 x 96-well plates
00188395 2 x Fast 96-well plates Toll-like Receptor Signaling 4 x 96 StellARray™ qPCR Array
Pathogen Recognition 4 x 96 StellARray™ qPCR Array 00188461 2 x 384-well plates
00188454 2 x 384-well plates Wnt Signaling 96 StellARray™ qPCR Array
Stem Cell 384 StellARray™ qPCR Array 00188197 2 x 96-well plates

Wnt Signaling 4 x 96 StellARray™ qPCR Array
Stress Response 96 StellARray™ qPCR Array
00188462 2 x 384-well plates
Stress Response 4 x 96 StellARray™ qPCR Array
00188455 2 x 384-well plates
T cell Receptor Signaling 96 StellARray™ qPCR Array

T cell Receptor Signaling 4 x 96 StellARray™ qPCR Array
00188456 2 x 384-well plates
T helper 17 (Th17) 96 StellARray™ qPCR Array

T helper 17 (Th17) 4 x 96 StellARray™ qPCR Array
00188457 2 x 384-well plates
216
BioAssays
Chapter 4
BioAssays
BioAssay Products and Services

Introduction 219
MycoZap™ Mycoplasma Elimination Reagent 222
ViaLight® Cell Proliferation and
Cytotoxicity BioAssay Kits 223
ToxiLight® Non-destructive Cytotoxicity BioAssay Kit 226
ApoGlow® Rapid Apoptosis Screening Kit 228
PKLight® HTS Protein Kinase Assay Kit 230
PDELight® HTS cAMP Phosphodiesterase Assay Kit 232
BioAssays
PPiLight® Inorganic Pyrophosphate Assay 234

AdipoRed™ Assay Reagent 236
CalciFluor™ Quantitative Bone Resorption Assay 239
BioAssay Accessory Products 241
Cell Analysis Reagents 242
G Protein-Coupled Receptors 245
BioAssay Services 246
BioAssays Frequently Asked Questions 247
New Products
218
BioAssays
BioAssays
When you need to perform high-throughput in vitro unprecedented performance. The bioassay product family
toxicology, drug discovery research, or assess the health provides an integrated assay platform for cell biology
of your cells, we have you covered. We offer a powerful line research that is complemented by our Clonetics® and
of products and services designed to deliver increased Poietics® Cells and Media. Assays are offered in kit format
speed, sensitivity, and convenience over conventional or can be supplied in bulk, thus providing appropriate
methods. Whether you’re studying cell proliferation, reagent quantities for each phase of the research or drug
apoptosis, cytotoxicity, cell differentiation, or enzyme discovery process.
activities, we have a line of bioassay kits that will deliver
BioAssay Selection Guide
Sensitivity Detection Plate

BioAssay Application Detection Limits Method Compatibility
MycoAlert® Mycoplasma Mycoplasma detection <50 cfu/ml Luminescence cuvette or 96
Detection Kit
ViaLight® Plus Kit Cell proliferation, cytotoxicity 10 cells Luminescence 96, 384
ViaLight® MDA Plus Kit Cell proliferation, cytotoxicity 1,000 bacterial Luminescence 96, 384
cells, 100 yeast
cells
ToxiLight® Kit Cytotoxicity 10 cells Luminescence 96, 384
ApoGlow® Screening Kit Apoptosis, necrosis 100 cells Luminescence 96
PKLight® HTS Protein Kinase Kinase activity <10 ng Luminescence 96, 384, 1536
Assay Kit
PDELight® HTS cAMP cAMP dependent <0.001 mU Luminescence 96, 384, 1536
Phosphodiesterase Assay Kit phosphodiesterase activity
PPiLight® Inorganic Detection of inorganic 0.02 µM Luminescence 96, 384
Pyrophosphate Assay phosphate
OsteoAssay™ Human Bone Bone resorption, osteoclast — — 96
Plate precursor differentiation,
osteoclast enzyme activity
OsteoLyse™ Assay Kit Bone resorption, osteoclast — Fluorescence 96
(Human Collagen) precursor differentiation
CalciFluor™ Quantitative Bone Bone resorption, osteoclast — Fluorescence 96
Resorption Assay precursor differentiation
OsteoImage™ Mineralization Bone formation — Fluorescence 96
Assay
219
MycoAlert® Mycoplasma Detection Kit
Detect mycoplasma contamination in less than 20 minutes
The MycoAlert® Mycoplasma Detection Kit is designed to Ordering Information

detect mycoplasma contamination in cell cultures. The
Cat. No. Quantity Price
MycoAlert® Kit is simple to use, is very fast to perform,
and gives reliable results, even with very low levels of MycoAlert® Mycoplasma Detection Kit
mycoplasma contamination. The speed, convenience, and LT07-118 10 tests
reliability of the assay now make mycoplasma detection LT07-218 25 tests
so easy that it can be performed at every cell passaging LT07-418 50 tests
to ensure that your cultures are clean and your results are
MycoAlert® Mycoplasma
LT07-318 100 tests

untainted.
MycoAlert® Assay Control Set
Detection Kit
The MycoAlert® Kit is a selective biochemical test that LT07-518 10 tests
exploits the activity of mycoplasmal enzymes. The

presence of these enzymes provides a rapid screening
procedure allowing sensitive detection of contaminating
mycoplasma in a test sample. The viable mycoplasma are Comparison of mycoplasma detection results of HepG2
lysed and the enzymes react with the MycoAlert® Substrate, cells infected with A. laidlawii
catalyzing the conversion of ADP to ATP. By measuring the A. laidlawii (cfu/ml) Culture Fluorescence PCR MycoAlert®
level of ATP in a sample both before and after the addition 0 Neg Neg Neg Neg
of the MycoAlert® Substrate, a ratio can be obtained which 20 Pos Pos Neg Pos
200 Pos Pos Pos Pos
is indicative of the presence or absence of mycoplasma. 20,000 Pos Pos Pos Pos
200,000 Pos Pos Neg Pos
The MycoAlert® Assay Control Set (sold separately) is Standard assay protocols were performed by an independent testing lab.
available with positive and negative controls for ensuring
proper assay performance. The control set does not
contain mycoplasma. ■ Applications
— Fast – Get results in less than 20 minutes to quickly – Mycoplasma contamination detection
assess the health of your cultures; other methods – Cell culture screening
take several hours to weeks to get results
— Convenient – Simply mix two reagents with 100 µl of ■ Storage Conditions
your culture supernatant and take two readings on 2°C – 8°C, do not freeze
your luminometer
— Universal – Detect all mycoplasma and acholeplasma Simple protocol
infections commonly contaminating cell cultures
— Sensitive – Detect <50 cfu/ml mycoplasma and avoid Cell culture supernatant (100 µl)
▼
false negative results often encountered with PCR and Add MycoAlert® Reagent
fluorescent staining procedures (Wait 5 min)
▼
— Complete – Each kit contains all of the reagents Measure luminescence
(Reading A)
needed to perform the assay, unlike commercial PCR ▼
kits, which require you to supply Taq Polymerase, Add MycoAlert® Substrate
(Incubate 10 min)
nucleotides, restriction enzymes, and agarose gel ▼
electrophoresis reagents (assay controls sold Measure luminescence
(Reading B)
separately) ▼
Ratio B/A >1 equals
MycoAlert® Kit is a mycoplasma detection assay. The assay and kits mycoplasma contamination
are covered by UK patent number GB2,357,336 and pending patent
applications.
220
MycoAlert® Mycoplasma Detection Kit
Continued
Assay kinetics of light output for infected and uninfected cells Specifications
10,000
Luminescence (RLU)
M.hyorhinis infected: B/A ration 114

Uninfected control: B/A ratio <1 Number of tests per kit: 10 tests (5 tests/bottle)
25 tests (5 tests/bottle)
1,000
50 tests (25 tests/bottle)
Reading A Reading B 100 tests (100 tests/bottle)
100 Detection limit: <50 cfu/ml
Assay time: <20 minutes
Detects: Mollicutes
MycoAlert® Mycoplasma
10
Operating temperature: Ambient

1 0 2 4 6 8 10 12 14 16 Recommended equipment: Cuvette or microplate luminometer
Detection Kit
Time(minutes) Liquid scintillation counter with
“luminescence” (i.e. out of coincidence)
mode.
Note: Absorbance readers, fluorometers, and ELISA plate readers are not
suitable instruments for the detection of bioluminescence. Check the
luminometer list at www.lonza.com for recommended luminometers.
Comparison of mycoplasma detection methods
Test Method
MycoAlert® Kit
PCR Amplification
Fluorescence
Staining
14-21 days
Culture Method
0 1 2 3 4 5 6
Time (hours)
For rapid, reliable mycoplasma detection, MycoAlert® Kits outperform

other procedures.
Related Products
MycoZap™ Mycoplasma Elimination Agent 222
221
MycoZap™ Mycoplasma Elimination Reagent
Reports are that 5 - 35% of all cells in continuous culture are Ordering Information
contaminated with mycoplasma. A cell line infection can
Cat. No. Size Price
affect every known process in the cell and can seriously
impact the reliability, reproducibility and consistency MycoZap™ Mycoplasma Elimination Reagent
of results. Additionally, mycoplasma infections can LT07-818 1 treatment
spread easily within the culture environment. To monitor LT07-918 5 treatments
for such infections, regular testing of cell lines should
be performed using Lonza’s MycoAlert® Mycoplasma Competitor 1 –
MycoZap™ Mycoplasma
Detection Kit. Where contamination has occurred, and the Effect on1 —
cell viability and and mycoplasma removal
Elimination Reagent
Competitor Effect on cell viability mycoplasma removal

sample absolutely cannot be discarded, the MycoZap™ 100 10000000

MycoAlert® ratio
Mycoplasma Elimination Reagent has been optimized to Mycoplasma detected
1000000
eliminate mycoplasma with minimal toxic effects on the 10

100000
cells. 10000
The MycoZap™ Reagent eliminates mycoplasma by using 1

1000
a combination of antibiotic and antimetabolic agents. No mycoplasma detected

100
This approach allows for a highly reliable and definite 10
elimination of mycoplasma that cannot be achieved by


the use of antibiotics alone.
Cultures should be tested with the MycoAlert® Mycoplasma
Competitor 2 –
Detection Kit at regular intervals for 4-6 weeks after
Effect on2 —cell
Competitor viability
Effect and and
on cell viability mycoplasma removal
mycoplasma removal
mycoplasma elimination to ensure fresh infections do
100 10000000
not arise. MycoZap™ Mycoplasma Elimination Reagent is:
MycoAlert® ratio

Mycoplasma detected
1000000
— Easy – simply add the reagent to your culture media 100000

10
— Universal – MycoZap™ Reagent can be used to 10000
eradicate mollicutes, including mycoplasma, 1000

1
acholeplasma, spiroplasma and entomoplasma No mycoplasma detected
100
species in cell cultures 10

— Thorough – the combination of antibiotic and untreated treated untreated viability treated viability
antimetabolic agents ensure total removal of the
contamination
— Complete – the kit contains all the required reagents MycoZap™ Reagent –
Effect
MycoZap™on cell viability
— Effect andandmycoplasma
mycoplasma removal
■ Applications 100 10000000 ViaLight® Plus RLUs
MycoAlert® ratio
– All cell culture

Mycoplasma detected
1000000
– Mycoplasma elimination 10
100000
10000
■ Storage Conditions 1000
2°C – 8°C 1
100
No mycoplasma detected
10
0.1 Day 0 Day 4 Day 8 Day 11 Day17 1
– Both competitors' products take at least 2 weeks to eliminate the

mycoplasma infection
– Competitor 2’s treatment adversely affects cell viability
– The MycoZap™ Reagent treatment eliminates mycoplasma in as few as
4 days
Related Products – The MycoZap™ Reagent treatment kills mycoplasma with minimal impact
on cell viability
222
ViaLight® Cell Proliferation and Cytotoxicity BioAssay Kits
Fast and accurate cell proliferation or cytotoxicity analysis
ViaLight® Cell Proliferation and Cytotoxicity BioAssay Ordering Information

Kits are designed to provide unprecedented speed and
sensitivity for cytotoxicity and cell proliferation studies,
and are safer than traditional radioactive methods. The ViaLight® Plus Cell Proliferation and Cytotoxicity
ViaLight® Kit incorporates bioluminescent detection of BioAssay Kit
cellular ATP as a measure of viability. These convenient, LT07-221 500 tests
homogeneous assays are scalable for high-throughput LT07-121 1,000 tests
applications in both 96- and 384-well formats, and can be LT07-321 10,000 tests
ViaLight® BioAssay Kits

run on a variety of luminmeters or scintillation counters. LT17-221 500 tests, with 5 white TC plates
ViaLight® Plus Cell Proliferation and Cytotoxicity ViaLight® HS Cell Proliferation and Cytotoxicity
BioAssay Kit BioAssay Kit
LT07-211 500 tests
The ViaLight® Plus Cell Proliferation and Cytotoxicity LT07-111 1,000 tests
BioAssay Kit is designed to deliver high, stable LT07-311 10,000 tests
luminescent signals for an extended period of time, for
greater experimental design flexibility. The easy, two-step ViaLight® MDA Plus Microbial Proliferation and
assay is scalable for high throughput applications and Cytotoxicity Kit
can be run on a variety of luminometers or scintillation LT07-122 1,000 tests
counters. LT07-322 10,000 tests
— Fast – Results from a 96-well plate can be processed

and analyzed in <15 minutes
— Sensitive – Detect as few as 10 cells allowing lower
seeding densities and more assays
— Convenient – Simply add two reagents directly to ViaLight® HS Cell Proliferation and Cytotoxicity
your culture well and read; no cell washing or medium BioAssay Kit
removal required The ViaLight® HS (High Sensitivity) Cell Proliferation and
— Flexible – Dynamic range of 5 decades and excellent Cytotoxicity BioAssay Kit, the original ATP viability assay,
performance with both adherent or suspension can detect less than 10 mammalian cells per microwell
cultures allows you to design the experiment for your and measures over a dynamic range of 5 decades.
needs The assay takes <15 minutes overall to measure all
— Robust – Stable bioluminescent signal (half-life >6 96 wells and is suitable for both adherent and
hours) facilitates manual or batch processing; unlike non-adherent cells.
MTT or Alamar Blue assays, ViaLight® Kits are not prone
to interference due to culture condition changes ViaLight® MDA Plus Microbial Proliferation and
Cytotoxicity Kit
The ViaLight® MDA Plus Microbial Proliferation and
Cytotoxicity Kit has been optimized for use with bacteria
or yeast. The basic reaction remains the same as the
ViaLight® Plus Kit, however, the lysis reagent has been
optimized for bacteria and yeast. Sensitivity is 1,000
bacterial cells or 100 yeast cells per well.
Continued on next page
223
Continued
ViaLight® Cell Proliferation and Cytotoxicity BioAssay Kits ViaLight® Plus BioAssay Kit signal stability
are: 1,000,000
Luminescence (RLU)
Highly sensitive – The high sensitivity of the ViaLight®
100,000
Plus and HS BioAssay Kits enable lower seeding densities
to be used, reducing the high costs of culture media and 10,000
plastics that other assays require.
1,000
— Fast – Results from a complete 96-well plate are
available in <15 minutes (including the cell lysis step) 100
for the ViaLight® Plus and HS BioAssay Kits; the same 10

number of tests with the tritiated thymidine method
would take 8 hours to complete 0 0 1 2 3 4 5
Time (hours)
6
100,000 cells/well 10,000 cells/well 1,000 cells/well

— Simple – ViaLight® Kits are simple to perform and
100 cells/well 10 cells/well
can be fully automated for laboratory robots using a
Extended luminescent signal stability (half life >6 hours) regardless of the
microplate luminometer number of cells used facilitates batch processing and ensures consistent
results.
— Robust – Stable bioluminescent signal (half-life >6
hrs) with the ViaLight® Plus BioAssay Kit at many
different cell concentrations facilitates manual or Comparison of signal stability over time
batch processing 160%
— Flexible – Dynamic range of 5 decades and specially Relative Luminescence

140%
designed kits for mammalian, yeast or bacterial cells, 120%
or manual and automated modes offer exceptional
100%
experimental design flexibility
80%
■ Applications
60%
– Cell proliferation studies 40%
– Cytotoxicity studies 20%
– Cell viability studies 0% 0 20 40 60 80 100 120 140 160 180 200 220
Time (minutes)
– High-throughput screening ViaLight® Plus Competitor
■ Storage Conditions Light output with ViaLight® Plus BioAssay Kit and a competitive luminescent
assay kit with A549 cells was compared over time. The consistent light
2°C – 8°C, do not freeze output, 10-fold lower background and exceptional lysis capabilities of the
ViaLight® BioAssay Kit ensure superior results.
ViaLight® Plus BioAssay Kit sensitivity and extended References

linearity 1. Crouch, S., Kozlowski, R., Slater, K., and Fletcher, J. (1993) The use of
100,000 ATP bioluminescence as a measure of cell proliferation and cytotoxicity.
Luminescence (RLU)
J Immunol Methods 160: 81 – 88.

2. Slater, K. (2001) Cytotoxicity tests for high throughput drug discovery.
10,000
Current Op. in Biotech. 12: 70 – 74.
3. Crouch, S. and Slater, K. (2001) High-throughput cytotoxicity screening:
1,000 T=1 min hit and miss. DDT 6 (Suppl.).
T=1 hour
T=2 hours
T=3 hours
100 T= 4 hours
T=5 hours
T=6 hours
10
Background
Related Products
0.1 0.1 10 100 1,000 10,000 100,000
Cells/Well
ATP Standard 241
Light output at various times after reagent addition and with increasing Clear Bottom, White Walled Tissue Culture Plates 241
numbers of K562 cells demonstrate the exceptional sensitivity and dynamic
range delivered by the ViaLight® Plus BioAssay Kit. ExPro™ Luminometer Injector Cleaning Solution 241
224
Continued
Specifications
Number of tests per kit: 500 (5 96-well plates)

1,000 (10 96-well plates)
10,000 (100 96-well plates)
Detection limit: ViaLight® Plus and HS BioAssay Kits – 10 mammalian cells per well
ViaLight® MDA Plus BioAssay Kit – 1,000 bacterial cells per well, 100 yeast cells per well
Assay time: ViaLight® Plus and HS BioAssay Kits – <15 minutes per plate
ViaLight® MDA Plus BioAssay Kit – <15 minutes per plate

Suitable cell types: ViaLight® Plus and HS BioAssay Kits – All mammalian cells, adherent and non-adherent
ViaLight® MDA Plus BioAssay Kit – All bacterial (excluding spores) and yeast cells
Linear range: Greater than 5 orders of magnitude
Reproducibility: Typical Coefficient of Variation (CV) is ≈6%
Correlation: Shows excellent correlation with tritiated thymidine (typically, R2 = 0.995, p<0.0001)
Recommended equipment: Microplate luminometer with or without reagent injectors
Microplate liquid scintillation counter with “luminescence” (i.e., out of coincidence) mode
Examples of cell types and treatments tested using ViaLight® BioAssay Kits
Cell Type Treatment

HL60, human acute promyelocytic leukemia Cold shock, Ara-C, camptothecin, etoposide, daunorubucin
Jurkat, human acute T-lymphoblastic leukemia Ara-C, etoposide
CEM7, human acute T-lymphoblastic leukemia Dexamethasone
U937, human histiocytic lymphoma Camptothecin
K562, human acute promyelocytic leukemia Daunorubicin
TF-1, human erythroleukemia +/- GM-CSF
B9, mouse IL-6 dependent +/- IL-6 (murine)
PC12, rat adrenal, pheochromocytoma +/- NGF (rat)
A549, human lung carcinoma Etoposide, staurosporine, ATP analogue
L929, mouse connective tissue TNF (murine)
NFS-60, myeloid G-CSF (murine)
HUVECs, human umbilical cord endothelial cells Camptothecin
Dermal fibroblasts Irradiation
Dendritic cells Etoposide, adriamicin
AML primary cell cultures Ara-C
Lymph node primary cell cultures Etoposide, Ara-C
MCF7, breast cancer Several cytotoxic agents
Uveal melanoma Several cytotoxic agents
H36, chicken lens Hydrogen peroxide
Human bladder carcinoma Mitomycin
225
ToxiLight® Non-destructive Cytotoxicity BioAssay Kit
Single-reagent, high content cytotoxicity assay
The ToxiLight® Non-destructive Cytotoxicity BioAssay Ordering Information

Kit is a bioluminescent, non-destructive cytolysis
assay kit designed to measure the release of the
enzyme, adenylate kinase (AK), from damaged cells. ToxiLight® Non-destructive Cytotoxicity BioAssay Kit
AK is a robust protein present in all eukaryotic cells, LT07-217 500 tests
which is released into the culture medium when LT07-117 1,000 tests
cells die. The enzyme actively phosphorylates ADP LT17-217 500 tests, with 5 white TC plates
ToxiLight® Non-destructive
to form ATP and the resultant ATP is then measured

Cytotoxicity BioAssay Kit
using the bioluminescent firefly luciferase reaction. As ToxiLight® 100% Lysis Control Set (sold separately)
the level of cytolysis increases, the amount of AK in the LT07-517 10 ml (200 tests)
supernatant also increases, which results in emission of

higher light intensity by the ToxiLight® Reagent.
Because the ToxiLight® BioAssay Kit exploits the fact that ■ Applications
AK is released from cells when they die, there is no need – Cytotoxicity studies
for cell lysis (unlike many other cytotoxicity assays).
Repeated samples of supernatant can therefore be taken – High content screening
over time without disrupting the cells themselves. This – Combination assays
allows for kinetic analysis of cell death. The ToxiLight® ■ Storage Conditions
BioAssay Kit also facilitates high content screening
by allowing other tests to be performed on the original 2°C – 8°C, do not freeze
cells. For example, the ToxiLight® BioAssay Kit is fully
compatible with reporter gene constructs, thus facilitating
measurement of cytotoxicity and gene expression in the
same sample.
All the components required for AK detection are contained
within a single reagent making the assay very simple to
perform. The kit provides outstanding sensitivity with a
detection limit of 10 cells per microwell with a dynamic
range of over 3 orders of magnitude.
A 100% Lysis Control Set (sold separately) is available for
determining the total AK activity in the reaction.
— Highly sensitive – Exploits the cyclic nature of the AK
reaction whereby a small amount of the AK enzyme
can generate high concentrations of ATP; this enables
the detection of very subtle changes in cytotoxicity
and reduces false negatives
— Non-destructive – Detects cellular AK present in cell
culture supernatants, eliminating the need to lyse cells
to perform the assay, and allowing multiple tests to be
performed on the same sample
— Simple – Assay requires the addition of a single reagent,
which can be added directly to wells in which cells are
growing, or to a small aliquot of culture supernatant
— Fast – Results from a 96-well plate can be processed
and analyzed in <10 minutes Related Products
— Flexible – Cell supernatants can be frozen with no loss Clear Bottom, White Walled Tissue Culture Plates 241
of AK activity, allowing for long-term kinetic studies ExPro™ Luminometer Injector Cleaning Solution 241
— Safe – No hazardous chemicals are required ViaLight® Plus Cell Proliferation and Cytotoxicity BioAssay Kit 223
226
ToxiLight® Non-destructive Cytotoxicity BioAssay Kit
Continued
Kinetics of cytotoxicity Identify dose-dependent activities in cells

70,000 30,000
ViaLight® RLUs
ToxiLight® RLUs
60,000
Luminescence (RLU)
60,000
25,000
50,000
50,000
20,000
40,000 40,000
15,000
30,000
30,000
ToxiLight® Non-destructive
10,000
Cytotoxicity BioAssay Kit

20,000
ViaLight® Plus
20,000
ToxiLight® 5,000
10,000
10,000 0 0 50 100 250 500 1000 2000 5000 0

Camptothecin (nM)
0 0 2 4 6 24
Time (hours)
Comparison of ViaLight® Plus and ToxiLight® Kits using HUVECs dosed with
camptothecin. The ATP levels indicated by the ViaLight® Plus RLUs reduce
Samples (20 µl) of culture supernatant from cells treated with camptothecin steadily in a dose-dependent manner. At the lower drug doses, the AK
were collected at various times and assayed for cytotoxicity. released from the cells is relatively low compared with that of the control,
only increasing dramatically at the highest drug doses.
ToxiLight® BioAssay Kit is sensitive to 10 cells Adenylate kinase is stable over 3 days
120,000
RLUs
Luminescence (RLU)
100,000 7000
R2 = 0.9979
80,000 6000
60,000 5000
40,000 4000
20,000 3000
0 2000
0 10,000 20,000 30,000 40,000 50,000
Cells/Well
1000
Exceptional sensitivity and wide dynamic range results in exceptional
experimental flexibility.
0
Day 1 Day 2 Day 3
Days after lysate
Jurkat cells were seeded at 105 cells/ml and immediately lysed using
the ToxiLight® 100% Lysis Reagent. The stability of the released AK was
measured at 24h, 48h, and 72h after release with no significant loss in
activity.
Specifications

1,000 (10 96-well plates)
10,000 (100 96-well plates)
Detection limit: 10 dead cells per well in homogeneous mode and 50 dead cells per well when the supernatant is
sampled from the wells
Assay time: 1 second integrated reading per sample
<15 minutes per 96-well plate
Suitable cell types: All mammalian cells, adherent and non-adherent
Linear range: Greater than 3 orders of magnitude
Reproducibility: Typical Coefficient of Variation (CV) ≈5%
Correlation: Shows excellent correlation with other membrane permeability assays such as propidium iodide
Recommended equipment: Microplate luminometer with or without reagent injectors
Microplate liquid scintillation counter with “luminescence” (i.e. out of coincidence) mode
227
ApoGlow® Rapid Apoptosis Screening Kit
Differentiate between apoptosis, necrosis and cell proliferation with a single assay
The ApoGlow® Rapid Apoptosis Screening Kit offers Ordering Information

sensitive, safe and rapid detection of apoptosis, necrosis
and cell proliferation in a convenient 96-well format that
can be fully automated. The ApoGlow® Screening Kit ApoGlow® Rapid Apoptosis Screening Kit
uses bioluminescence catalyzed by the firefly enzyme LT07-115 200 tests
luciferase to measure intracellular changes in ADP/
ATP ratios. ApoGlow® Assays are run on luminometers ■ Applications
ApoGlow® Rapid Apoptosis
or scintillation counters (luminometers with reagent

injection facilities are strongly recommended) and are – Apoptosis screening
designed for high-throughput applications. – Necrosis screening
Screening Kit
— Efficient – One of three possible outcomes – apoptosis, – Cell proliferation studies

necrosis, or proliferation – can be determined from a
single assay when compared to a drug-free control, ■ Storage Conditions
saving time and reagent costs 2°C – 8°C, do not freeze
— Reliable – Data from ApoGlow® Kits correlates with
established apoptosis assays including TUNEL,
propidium iodide, Annexin V, and caspase assays
References
— Versatile – Enables adherent and non-adherent cells
1. Bradbury, D., Simmons, T., Slater, K., and Crouch, S. (2000) Measurement
to be tested with the same degree of convenience and of the ADP:ATP ratio in human leukaemic cell lines can be used as an
level of performance (as opposed to Annexin V and indicator of cell viability, necrosis and apoptosis. J. Immunol. Methods
243:167-190.
TUNEL, which are unsuitable for adherent cells) 2. Slater, K. (2001) Cytotoxicity tests for high throughput drug discovery.
Current Opinion in Biotechnology 12:70-74.
— Rapid – All samples in a 96-well plate can be processed 3. Crouch, S., and Slater, K. (2001) High-throughput cytotoxicity screening:
and analyzed in as little as 20 minutes hit and miss. DDT 6: (Suppl) S48-S53.
— Suitable for automation – The ApoGlow® Screening Kit

is compatible with robotic sample processors, reducing
research time and increasing cost-effectiveness in cell
viability assays
ApoGlow® Screening Kit is an apoptosis assay. The assay and kits are
covered by US patent number 6,004,767 and UK patent number
GB2,323,167.
Apoptosis detection using the ApoGlow® Screening Kit vs.

flow cytometry
ADP/ATP Ratio
0.35 60
% Apoptosis
Ratio
% Apoptosis (PI)
0.3 50
0.25 40
0.2 30
0.15 20
0.1 10
0.05 0 10 25 50 75 100 150 200 0
Dexamethasone (nM)
Related Products
Graph shows a comparison between ApoGlow® Screening Kit ratios and %
apoptosis determined by flow cytometric analysis of the sub G0/G1 peak ATP Standard 241
obtained with PI staining. CEM-7 cells were incubated with increasing Clear Bottom, White Walled Tissue Culture Plates 241
concentrations of dexamethasone for 72 hours. The data is shown as means
+/- SEM for 83 separate experiments. ExPro™ Luminometer Injector Cleaning Solution 241
ViaLight® Cell Proliferation and Cytotoxicity BioAssay Kits 223
228
ApoGlow® Rapid Apoptosis Screening Kit
Continued
Specifications

Detection limit: 100 mammalian cells per well, 10% of necrotic cells in an actively proliferating population
Assay time: 20 minutes per plate
Suitable cell types: All mammalian cells, adherent and non-adherent.
ApoGlow® Rapid Apoptosis

Reproducibility: Typical Coefficient of Variation (CV) is <10%
Correlation: Shows excellent correlation with PI (r2 = 0.831, p<0.00001); TUNEL (r2 = 0.799, p<0.0001)
Recommended equipment: Microplate luminometer with reagent injectors. (Luminometers without reagent injectors may
Screening Kit
also be used, but sample throughput is compromised); microplate liquid scintillation counter with
“luminescence” (i.e. out of coincidence) model
Apoptotic Models Tested Using the ApoGlow® Rapid Apoptosis Screening Kit
Cell Type Treatment

Suspension cells
B-9, mouse IL-6 dependent Removal of IL-6
BCP-1, human B lymphoma Serum withdrawal
CEM7, human acute T-lymphoblastic leukemia Dexamethasone, etoposide
Dermal fibroblasts UV radiation
HL60, human acute promyelocytic leukemia Camptothecin, Ara-C, etoposide, ceramide
Jurkat, human acute T-lymphoblastic leukemia Ara-C, dexamethasone, etoposide, staurosporine, vincristine
TF-1, human erythroleukemia Removal of GM-CSF
K562, human acute promyelocytic leukemia Etoposide
U937, human histiocytic lymphoma Ara-C, camptothecin, vincristine
Adherent cells
A549, human lung carcinoma Vincristine, staurosporine, etoposide
CHO, Chinese hamster ovary Lonomycin, staurosporine
HepG2, human hepatocyte carcinoma Vinblastine
H-36, chicken lens cells Hydrogen peroxide
L929, mouse fibroblasts TNFα
PC12, rat adrenal pheochromocytoma cells Removal of NGF, Ara-C
SWISS 3T3, mouse embryo fibroblasts Vincristine
T47D, human breast carcinoma Staurosporine
MCF7, human breast adenocarcinoma Unknown compounds
Primary cultures
Peripheral blood mononuclear cells Serum withdrawal
Neutrophils, peripheral blood polymorphonuclear cells Serum withdrawal
AML blast cells, acute myeloid leukemia cell line Ara-C, etoposide
Islets of Langerhans, porcine and human Biocompatibility agents; alginates
B cell lymphoma Ara-C, etoposide
Breast carcinoma cells Isolation from biopsies
229
PKLight® HTS Protein Kinase Assay Kit
Rapid, generic assay for protein kinases
The PKLight® HTS Protein Kinase Assay Kit is designed Ordering Information
for rapid screening of protein kinase activity. The assay
utilizes the bioluminescent measurement of ATP to provide
a generic endpoint detection system for determination PKLight® HTS Protein Kinase Assay Kit
of a wide range of protein kinases. The PKLight® Kit is LT07-500 500 tests
non-radioactive, homogeneous, robust and simple, and is LT07-501 5,000 tests
suitable for the screening of potentially all protein kinases 10 +
in 96-, 384- and 1536-well formats. Bulk reagents for high-throughput screening applications are also available.
PKLight® HTS Protein
Please Inquire.
Kinase Assay Kit
During a kinase reaction, the level of free ATP in the ■ Applications

reaction mixture drops as the γ-phosphate is cleaved and – Protein kinase activity screening
transferred to the kinase substrate. This drop in free ATP
can then be measured using a bioluminescent reaction, ■ Storage Conditions
which is quantified by a reduction in light emission. 2°C – 8°C, do not freeze
The PKLight® Kit is catalyzed by the firefly luciferase

enzyme and provides speed, sensitivity and convenience. References
Lonza supplies a patented reagent system containing 1. Singh, P., Harden, B.J., Lillywhite, B.J., and Broad, P.M. (2004) Identification
luciferase and luciferin, which emits a stable light signal, of kinase inhibitions by an ATP depletion method. Assay and Drug
Development Technologies 2: 161-169.
the intensity of which is proportional to the concentration
of ATP present in the well.
— Homogeneous assay – Add only one reagent following
completion of the kinase reaction
— Generic platform – Single endpoint for all kinase/ ATP levels drop as a result of kinase activity
substrate pairs, including serine/threonine, tyrosine
and lipid kinases
— Safe – Bioluminescent assay eliminates hazardous
radioactivity and minimizes disposal costs
— Rapid – Read an entire plate in less than 3 minutes
— Stable light emission – Extended light output is perfect
for batch processing applications
— Highly sensitive – Detect low levels of protein kinase
PKLight® Kit is a protein kinase assay. The assay and kits are covered by
UK patent number 2,375,171, US patent numbers 6,599,711 and 6,911,319,
Swiss patent number CH694,096, and pending patent applications.
Related Products
Clear Bottom, White-walled Tissue Culture Plates 241
ExPro™ Luminometer Injector Cleaning Solution 241
PDELight® HTS cAMP Phosphodiesterase Assay Kit 232
230
PKLight® HTS Protein Kinase Assay Kit
Continued
PKLight® Assay results with protein kinase SAPK2a Kinase inhibitor (SB203580) inhibition of Kinase p38B
300,000 280,000
Luminescence (RLU)
Luminescence (RLU)
260,000
250,000
240,000
200,000 220,000
200,000
150,000
180,000
100,000 160,000
PKLight® HTS Protein

140,000
Kinase Assay Kit

50,000
120,000
0 100,000
0 1.25 2.50 5.00 10.00 20.00 40.00 Neg 0 0.1 1 10 100 1,000 10,000
Control (SB203580) nM
(SAPK2 A) µg/ml
Concentration dependent consumption of ATP (starting concentration 10 100 ng of kinase was used to phosphorylate 10 µg of dephosphorylated
µM) by SAPK2α mediated phosphorylation of myelin basic protein (10 µg myelin basic protein in the presence of 10 µM ATP over a 1 hour incubation
in reaction mixture) as measured using the PKLight® HTS Protein Kinase period. A concentration dependent effect can clearly be observed using the
Assay Kit. assay. Western blot data clearly verifies the phosphorylation event.
Specifications
Number of tests per kit: 500 (500 assay points per 96- or 384-well plate)
5,000 (5,000 assay points per 96- or 384-well plate)
ATP range: <1 µM – 20 µM
Assay time: < 3 minutes per plate
Protein kinases: Serine/threonine, tyrosine, lipid kinases
Reproducibility: Typical Coefficient of Variation (CV) is <5%. Z´ Factor is >0.7
Recommended equipment: Microplate luminometer
231
PDELight® HTS cAMP Phosphodiesterase Assay Kit
Rapid, generic assay for cAMP dependent phosphodiesterases
The PDELight® HTS cAMP Phosphodiesterase Assay Kit is Ordering Information

a generic, homogeneous assay designed for use in high
throughput screening to identify inhibitors of phospho-
diesterse activity and IC50 determinations. The assay PDELight® HTS cAMP Phosphodiesterase Assay Kit
utilizes a robust and highly sensitive luciferase-based LT07-600 500 tests
bioluminescent system to quantify the AMP produced
Bulk reagents for high-throughput screening applications are also available.
from the hydrolysis of cyclic AMP by phosphodiesterases. Please Inquire.
The AMP is directly converted to ATP and quantitated as
■ Applications
PDELight® HTS cAMP
light, with nearly a photon of light emitted for every

molecule of ATP produced. The assay is sensitive, robust – cAMP dependent phosphodiesterase activity
and reproducible. Unlike other phosphodiesterase assays, screening
Assay Kit
the PDELight® Kit does not require costly radioactivity, – IC50 determinations
the use of beads, modified substrates, or antibodies.
The PDELight® Kit is ideally suited for primary and 2°C – 8°C, do not freeze
secondary screening of cAMP dependent phospho-
diesterases.
— Simple, homogeneous assay – A single reagent is Linear detection of AMP
added to the completed reaction and read 107
Luminescence (RLU)
r2 = 0.9992
— Generic platform – The same assay can be used for all
106
cAMP dependent phosphodiesterases
— Non-radioactive – The assay contains no hazardous 105 AMP
cAMP
reagents, eliminating costly disposal expenses
104
— Rapid assay – Complete a 384-well plate in less than
3 minutes 103
— Bioluminescent sensitivity – Use small amounts of

enzyme in 96-, 384-, or 1536-well formats 102 0.01 0.1 1 10 100
AMP or cAMP (µM)
— Reproducible, robust assay – Low false positive rates,
The PDELight® Kit measures the AMP produced as a result of
typical Z´ values > 0.8, and few artifacts result in good phosphodiesterase activity. The PDELight® Detection Reagent measures
clean hits AMP up to 20 µM. The PDELight® Detection Reagent is specific for AMP and
not cAMP.
PDELight® Kit protocol Reproducible, high signal to background ratios

1,600,000
Luminescence (RLU)
10 µl of inhibitor Z´ = 0.86 S/B = 28.3

(40 µM in 10% DMSO)
1,750,000
10 µl phosphodiesterase
20 µl of cAMP substrate (40 µM) 800,000

(Incubate for 30 – 60 min
at room temp)
400,000
20 µl of PDELight®
Detection Reagent
(Incubate for 10 min 0
at room temp)
0 10 20 30 40 50
Measure luminescence Sample No.
(0.1 – 1 second/well)
Signal (S) and background (B) determinations were assessed using a single
phosphodiesterase demonstrating typical high quality data. Z´ results are
typically greater than 0.8 with excellent signal to background ratios.
232
PDELight® HTS cAMP Phosphodiesterase Assay Kit
Continued
Rapid screening and IC50 determinations with the PDELight® Assay Kit
100 1,000,000
% Inhibition
Luminescence (RLU)
MMPX
80 3-IBMX
IC50 = 6.2 µM
750,000
60
40 500,000
20
250,000
PDELight® HTS cAMP

0
-20
0
Assay Kit
-40 0.01 0.1 1 10 100 1,000
Compounds
IBMX (µM)
Left Image. A library containing 150 pharmacologically active compounds was screened using the PDELight® Kit. The compounds MMPX and 3-IBMX were identified
as inhibiting phosphodiesterase activity greater than 50% at 10 µM. Right Image. An IC50 of 6.2 µM was determined for 3-IBMX.
Specifications
Number of tests per kit: 500 (500 assay points per 96- or 384-well plate)
AMP range: <10 nM – 20 µM
Assay time: < 3 minutes per plate
Phosphodiesterases: cAMP phosphodiesterases
Reproducibility: Typical Coefficient of Variation (CV) is <5%. Typical Z´ value > 0.8
Recommended equipment: Microplate luminometer
233
PPiLight® Inorganic Pyrophosphate Assay
Inorganic pyrophosphate (also referred to as diphosphate, Ordering Information

pyrophosphoric acid or PPi) is a small diphosphate
molecule that is required as a substrate or is the product Cat. No. Size Price
formed from a number of different enzymatic reactions. PPiLight® Inorganic Pyrophosphate Assay
Enzymes that utilize PPi as a substrate may include LT07-610 500 tests
phosphotransferases (EC 2.7) and pyrophosphatases (EC
3.6.1.1). Enzymes that generate PPi are more numerous ■ Applications
and may include cyclases (EC 4.6.1), hydrolases (EC 3) – Measure activity of:
Pyrophosphate Assay
and ligases (EC 6).

PPiLight® Inorganic
– Phosphotransferases
The PPiLight® Inorganic Pyrophosphate Assay is a – Pyrophosphatases
non-radioactive bioluminescent assay for the detection – Cyclases
of inorganic pyrophosphate. In the presence of PPi, the
detection reagent catalyses the conversion of AMP to – Ligases
ATP. The assay uses luciferase, which produces light from – Hydrolases
the newly formed ATP and luciferin following the reaction – DNA polymerases
schematic shown below.
— Fast – Measure enzyme activity via pyrophosphate
consumption in 1 hour 2°C – 8°C
— Simple – The 2-step, luminescent assay doesn’t require

radioactive substrates, beads, detection antibodies or Bioluminescent reaction
modified substrates
— Wide detection range – Sensitive to 0.02 µM with a PPi + AMP
linear range to 10 µM ▼
ATP
— Versatile – Scalable to 96-, 384-, 1586-well formats
▼
Luciferin + Mg2+
▼
Luciferase
Oxyluciferin + AMP + PPi + CO2 + Light
PPiLight® Inorganic Pyrophosphate Assay protocol
Prepare reaction mixture in an appropriate volume.

▼ ▼
Continuous Kinetics Convert and Detect
▼ ▼
Add PPiLight® Add PPiLight® Converting
Converting & Detection Reagent to sample.
Reagent Mix to sample. Incubate for 30 min.
Incubate for ▼
desired period of
measurement. Add PPiLight® Detection
Reagent to sample.
Incubate for minimum
▼ of 30 min.
▼
Measure luminescence. Measure luminescence.
continued on next page
234
PPiLight® Inorganic Pyrophosphate Assay
Continued
Light produced directly proportional to PPi present Light increases proportionally to PPi concentration
8,000,000
RLUs
1,600,000
RLUs
6,000,000 1,200,000
4,000,000 800,000
300,000
200,000
0.156
Pyrophosphate Assay
2,000,000 100,000 400,000 0.313
PPiLight® Inorganic
PPi (µM)
0.625
0
0.0 0.1 0.2 0.3 0.4 1.25
0 0.0 2.5 5.0 7.5 10.0 12.5 0 0 10 20 30 40 50 60 70

PPi µM Mins
Typical linear PPi standard curve using the PPiLight® Inorganic Pyrophosphate PPi signal increases over time at a steady, proportional rate as PPi
Assay. Sensitivity is typically 0.02 µM to 10 µM with r 2 values >0.95. concentration increases. Signal linearity is constant throughout a 1 hour
incubation.
Linear signal Specifications

1,600,000
RLUs
Number of tests: 500 Tests in 96- or 384-well plates

PPi range: 0.02 µM – 10 µM in a 100ul sample
1,200,000
Assay time: 1 hour
0 0.9994 Operating temperature: Ambient
800,000 10 0.9993
20 0.9997
Reproducibility: r 2 value > 0.95
30 0.9996
40 0.9998
400,000
50 0.9999
60 0.9998
0 0.00 0.25 0.50 0.75 1.00 1.25 1.50

PPi µM
The linearity of the signal generated with varying concentrations of PPi

was assessed over 1 hour. Linearity is not affected by PPi concentration
increase.
235
AdipoRed™ Assay Reagent
Quantify intracellular lipid accumulation
The AdipoRed™ Assay Reagent is designed for assessing Ordering Information

the effect of compounds on the differentiation of
Cat. No. Size Price
preadipocytes or on lipid utilization in mature adipocytes.
The lipophilic AdipoRed™ Assay Reagent specifically AdipoRed™ Assay Reagent
partitions into the fat droplets of differentiated adipocytes PT-7009 5 × 4.0 ml
and fluoresces at 572 nm.
This objective, high-throughput, homogeneous, fluores-

cence-based assay quantifies the accumulation of
intracellular triglycerides and provides significant
Assay Reagent
AdipoRed™
advantages to drug discovery efforts in the field of obesity

and diabetes. It is more sensitive than other methods
such as the Oil Red O assay, and is much faster and easier
than Northern and Western blots.
— Convenient – Simply replace cell culture medium with
PBS, add AdipoRed™ Reagent and read in a standard
fluorimeter
— Fast – Process an entire 96-well plate in as little as Preadipocytes
20 minutes
— Effective – Provides objective, high throughput ■ Applications
measurement of the accumulation of intracellular – Differentiation of preadipocytes
triglycerides, with high signal-to-noise ratios
– Lipid utilization
Room temperature
Inhibition of adipocyte differentiation assayed with

AdipoRed™ Assay Reagent Quantitation of intracellular triglyceride accumulation
10,000
Fluorescence
Fluorescence
80,000
8,000
60,000
6,000
40,000
4,000
20,000
2,000
0 0 0 4 7 10
Day 0 Day 4 Day 4 Day 4
TNFA Mifeprestone Days of Induction
Poietics® Primary Human Preadipocytes were induced to differentiate in Poietics® Primary Human Preadipocytes were induced to differentiate and
the presence of TNFα, Mifepristone or no inhibitor. Lipid accumulation was assayed using the AdipoRed™ Assay Reagent.
assayed after 4 days in culture.
Related Products
Human Subcutaneous or Visceral Preadipocyte Cells 91
PGM™-2 Preadipocyte Growth Medium-2 BulletKit® 91
236
OsteoAssay™ Human Bone Plate
Measure osteoclastic bone resorption
The OsteoAssay™ Human Bone Plate provides a thin Ordering Information

layer of adherent human bone for the culture of primary
Cat. No. Size Price
human or non-human osteoclasts, osteoclast precursors,
and immortalized cell lines. Cells can be stained with OsteoAssay™ Human Bone Plate
standard cytochemical (e.g. TRAP) or immunofluorescent PA-1000 96 wells
techniques. Assays for measuring bone resorption, and/or
enzyme activity can be performed easily by sampling the
cell culture supernatant.
Time course of in vitro osteoclast resorptive activity
Human Bone Plate

— Convenient – Ready-to-use plates with human bone assayed with the OsteoAssay™ Plate and a Telopeptide
chips attached to wells eliminates the need for dentine
OsteoAssay™
EIA Kit
or animal bone slices 400
Collagen Telopeptide (nM)

— Simple – Cells can be seeded onto the surface of the Control
Differentiated
OsteoAssay™ Plate as used in traditional cell culture 300
protocols
— Flexible – Can be used with a variety of cell types and 200
cell-based assays
— Novel – Contains real human bone for more biologically 100
relevant results
■ Applications 0
24 hr 48 hr 96 hr
– Bone resorption The release of collagen peptides from the OsteoAssay™ Plate by
differentiating primary human osteoclasts is linear with time. Poietics®
– Osteoclast precursor differentiation Osteoclast Precursors were seeded onto an OsteoAssay™ Plate at 10,000
cells/well and cultured in medium containing M-CSF +/- soluble RANK ligand.
– Osteoclast enzymatic activity After 5 days of culture, the medium was renewed. Samples of supernatant
were harvested after an additional 24, 48 and 96 hours and used in an EIA
■ Storage Conditions assay for a telopeptide.
-20°C
OsteoAssay™ Plate is superior to dentine slices

Collagen Helical Peptide (ng/ml)
160
140 OsteoAssay™ Plate

Dentine disc
120
100
80
60
40
20
0 Differentiated Control
Comparison of primary human osteoclast function (in vitro bone

degradation) when cultured on an OsteoAssay™ Plate vs. dentine slices.
Poietics® Human Osteoclast Precursors were seeded at 10,000 cells/well in
the presence of either M-CSF alone (undifferentiated control) or both M-CSF
and soluble RANK ligand (differentiated) for 5 days. Media were renewed
after 5 days and supernatants were harvested after an additional 1 day of
culture and assayed for collagen peptides. Related Products
OCP Osteoclast Precursor BulletKit® 92
Human Osteoclast Precursors 92
CalciFluor™ Quantiative Bone Resorption Assay 239
237
OsteoLyse™ Assay Kit (Human Collagen)
Measure bone resorption in minutes
The OsteoLyse™ Assay Kit provides easy-to-use reagents Ordering Information

for quantitatively measuring in vitro osteoclast-mediated
bone matrix resorption in a high-throughput format.
The kit includes a 96-well cell culture plate coated with OsteoLyse™ Assay Kit (Human Collagen)
europium-labeled human Type I collagen, and a bottle of PA-1500 96 tests
Fluorophore Releasing Agent. Osteoclasts can be seeded
onto the OsteoLyse™ Plate using traditional cell culture
protocols. The assay directly measures the release of ■ Applications
OsteoLyse™ Assay Kit
europium-labeled collagen fragments (resorptive activity) – Osteoporosis

(Human Collagen)
into the osteoclast cell culture supernatant via time

resolved fluorescence. – Bone resorption
— Convenient – Human collagen is bound to wells in the – Osteoclast precursor differentiation

plate eliminating the need to purchase bone matrices – Mature osteoclast enzyme activity
separately – Cancer research: metastasis/collagen degradation
— Easy-to-use – Cells can be seeded onto the surface of ■ Storage Conditions
the OsteoLyse™ Plate as used in traditional cell culture
protocols 4°C – 8°C
— Homogeneous – Resorptive activity is easily measured

by simply sampling the cell culture supernatant and
counting via time-resolved fluorescence
Human osteoclast activity measured by collagen release A comparison of TRAP and OsteoLyse™ Assay Kits in
using the OsteoLyse™ Assay Kit alendronate-mediated osteoclast function
1,200,000 250 1.6
Multinucleated TRAP-Positive Cells
Activity (RFU)
OsteoLyse™ (RFU X 106)

Differentiated
Undifferentiated controls 1.4
1,000,000 200
1.2
800,000 1.0
150
0.8
600,000
100
0.6
400,000 TRAP stain
OsteoLyse™ 0.4
50
200,000 0.2
0 0
0 0.12 0.23 0.47 0.94 1.88 3.75 8 15
0 Day 7 Day 8 Day 9 Day 10 Alendronate (mM)
Time in culture
Poietics® Primary Human Osteoclast Precursors were seeded onto an Poietics® Primary Human Osteoclast Precursors were seeded onto an
OsteoLyse™ Plate at 10,000 cells/well and differentiated with M-CSF and OsteoLyse™ Plate at 10,000 cells/well and differentiated with M-CSF and
soluble RANK ligand. At days 7, 8, 9 and 10 of culture, 10 µl of supernatant soluble RANK ligand in the presence of interferon γ. At day 10 of culture,
was removed and counted. The blue bars represent counts obtained when 10 µl of supernatant was removed and counted. The red line denotes TRAP
the precursors were cultured with M-CSF only. data (day 10 multinucleated TRAP-positive cells/well) while the green line
represents OsteoLyse™ Assay data.
Related Products
CalciFluor™ Quantiative Bone Resorption Assay 239
238
CalciFluor™ Quantitative Bone Resorption Assay
Quantitative measurement of bone resorption
If you are evaluating osteoclast mediated bone degrada- Ordering Information

tion, the CalciFluor™ Quantitative Bone Resorption Assay
Cat. No. Size Price
offers a faster, simpler, more economical, fluorescent
assay with results in one hour with four easy steps. CalciFluor™ Quantitative Bone Resorption Assay
PA-4490 3 × 96 wells
Replace your TRAP, bone pitting, CrossLaps® CTx Assay or
Helical Peptide EIA assay with the rapid, easy CaliciFluor™
Assay.
CalciFluor™ Quantitative
Bone Resorption Assay
CalciFluor™ Assay works well with human osteoclast
precursors and murine bone marrow mononuclear cells.
(Fig. 1a, 1b)
Measurement of Osteoclast-Mediated Bone Degradation using CalciFluor™ Assay
Poietics® Human Osteoclast Precursor Cells Murine Bone Marrow Mononuclear Cells
Figure 1a Figure 1b
1.4
Calcium (mM)
Calcium (mM)
control
differentiated
0.8 control 1.2
differentiated
1.0
0.6
0.8
0.4
0.6
0.2
0.4
0.2
0.0 8 9 10
Time of sampling (days)
0.0 11 12 13
Time of sampling (days)
-0.2
-0.2
Figure 1a. Human OCPs were seeded onto a human bone substrate at 10,000 Figure 1b. Murine BM-MNCs were seeded into a T-25 flask and cultured
cells/well and cultured with M-CSF +/- sRANKL. Medium was renewed on overnight. Nonadherent cells were harvested and seeded onto a human
day 7 and on days 8, 9, and 10 of culture, supernatants were removed and bone substrate at 50,000 cells/well and cultured with M-CSF +/- sRANKL.
assayed for calcium. Medium was renewed on days 5 and 10 of culture and on days 11, 12, and
13 supernatants were removed and assayed for calcium.
CaliciFluor™ Assay vs. other assays
CalciFluor™ Assay Helical Peptide EIA CrossLaps® CTx

Format: 96-well plate 96-well plate 96-well plate
Kit Size: 3 x 96-well plates 1 x 96-well plate 1 x 96-well plate
Measures: Calcium Type I collagen helical peptide α1(I) C-telopeptide fragments of collagen
620-633 type I
Sample Amount: 10 µl 20 µl 30 µl
Assay Time: 1 hour 24 hours 4-5 hours
Number of Steps: 4 steps 8 steps 9 steps
Measurement Fluorescence at 570 nm Absorbance at 405 nm Absorbance at 450 nm
Calculation of Data: Excel® Requires special software Excel®
Related Products
239
OsteoImage™ Mineralization Assay
Rapid, flourescent assay for bone mineralization
The OsteoImage™ Mineralization Assay is a rapid, The OsteoImage™ Mineralization Assay can quantitate
fluorescent, in vitro assay for assessing bone cell in vitro mineralization by osteogenic stem cells,
mineralization. The OsteoImage™ Assay can quantitate primary osteoblasts, and osteoblast-like cell lines
in vitro mineralization by osteogenic stem cells, primary (Figure 1). The assay is sufficiently sensitive to detect
osteoblasts, and osteoblast-like cell lines. It is based the time-dependent increases in mineralization in
on specific binding of the fluorescent OsteoImage™ differentiating osteoblast cultures (Figure 2).
Staining Reagent to the hydroxyapatite portion of bone-
like nodules deposited by cells. Works with stem cells, primary cells, and cell lines
Mineralization Assay
1,000
RFU (492/520)
Unlike typical histochemical methods such as von Kossa Undifferentiated
and Alizarin red, neither of which is hydroxyapatite Differentiated
OsteoImage™
800
specific, the OsteoImage™ Assay eliminates multiple
steps or tedious extraction steps. 600
The OsteoImage™ Assay is the newest addition to Lonza’s

400
line of products for bone research. Increase the speed,
sensitivity and ease of measuring mineralization in
200
your cell cultures with the OsteoImage™ Mineralization
Assay. 0
UMR-106, Day 7 Saos-2, Day 11 NHOst, Day 21 hMSC, Day 28
Figure 1. Osteoblast cell lines, Clonetics® NHOst-Normal Human Osteoblasts, and

osteoblast-differentiated Poietics® hMSC Human Mesenchymal Stem Cells were
n The OsteoImage™ Mineralization Assay: evaluated for mineralization with the OsteoImage™ Mineralization Assay on a
96-well plate reader.
– Delivers qualitative, visual fluorescent microscopy or
quantitative plate reader results Detects mineralization with time
– Can be used with primary osteoblasts, osteoblast 600
RFU (492/520)
Undifferentiated
stem cells, and osteoblast cell lines 500
Differentiated
– Measures hydroxyapatite, similar to real bone

400
– Completed in less than 90 minutes, without tedious

300
extractions
200
– Sensitive enough to detect time-dependent
increases in mineralization in differentiating cells 100
– Scalable for use in 6-well up to 96-well plates 0 Day 7 Day 14 Day 21

Day of Assessment
Figure 2. NHOst-Normal Human Osteoblasts were seeded at 3,200 cells/well in a

96-well plate. Cells were cultured as undifferentiated control cells or with differentia-
tion factors. Mineralization was quantitated on a plate reader after staining with the
Ordering Information OsteoImage™ Assay on days 7, 14 and 21.
Cat. No. Size Price n Simple Protocol

OsteoImage™ Mineralization Assay Culture cells
PA-1503 5 x 96 wells ▼
Remove media and wash with PBS
▼
Fix cells
(20 min and wash)
Related Products ▼
Add OsteoImage™ Staining Reagent
OCP Osteoclast Precursor BulletKit® 92 (incubate 30 min)
Human Osteoclast Precursors 92 ▼
Wash 3 times
▼
OsteoLyse™ Assay Kit (Human Collagen) 238 View under fluorescence microscope
OsteoImage™ Mineralization Assay 239 or read on plate reader
240
BioAssay Accessory Products
Clear Bottom, White Walled Tissue Culture Plates ExPro™ Luminometer Injector Cleaning Solution
Clear Bottom White Walled Tissue Culture Plates are white- ExPro™ Luminometer Injector Cleaning Solution is a
walled 96-well plastic plates designed specifically for use specially formulated detergent specifically designed for
with any bioluminescent bioassay kit. bioluminescent applications. ExPro™ Solution is intended
to clean injector lines (e.g., on luminometers and sample
Ordering Information processors) between running different luminescent
applications where there is a risk of transferring reagent
Cat. No. Size Price components between different assays. For example,
Clear Bottom, White Walled Tissue Culture Plates reagents used in ATP measurement (i.e., ViaLight®
LT27-102 25 plates BioAssay Kits) contain high levels of luciferase, which
BioAssay Accessory
can contaminate luciferase assays when used in reporter
gene analysis. ExPro™ Solution will neutralize the
Products
ATP Standard luciferase enzyme and prevent the risk of contaminating
one assay with reagents used in a different assay.
The ATP Standard is a specialized aqueous preparation of
adenosine triphosphate (ATP) and is primarily intended
NOTE: ExPro™ Solution has only been tested with luciferase-
for use in research to calibrate ATP assays based on the
containing assays. Performance is not guaranteed for
luciferase bioluminescence technique. Each vial contains
other applications.
5 ml of 10 µM ATP.
2°C – 8°C
-20°C
ATP Standard ExPro™ Luminometer Injector Cleaning Solution

LT27-008 5 ml LT27-040 50 ml
241
Cell Analysis Reagents
Fluorescent reagents selected for optimal performance with Clonetics® and Poietics® Primary Cells
Reagents for Cellular Staining Ordering Information

Fluorescent probes for cells enable certain structures to Cat. No. Size Price
be visualized in cell-based assays e.g. viability/cytotoxity
assays, fluorescent microscopy, and flow cytometry. Phalloidin, Alexa Fluor® 488 Conjugate
The development and improvement of these probes have PA-3010 150 U
advanced the studies of different cell types including primary
cells from different organs. Cell permeant and impermeant CellTracker™ Green Fluorescent Probe
fluorescent probes are available for 2 and 3 color stains PA-3011 250 µg
e.g. nucleic acid, organelles and cytoskeletal structures.
CellTracker™ Orange Fluorescent Probe

Phalloidin, Alexa Fluor® 488 Conjugate PA-3012 250 µg
Phalloidin, Alexa Fluor® 488 Conjugate stains for F-actin MitoTracker™ Red CMxRos Mitochondrial Probe
at nanomolar concentrations. Labeled phalloidin has the PA-3017 5 × 50 µl
same affinity for large and small filaments, binding in a
stoichiometric ratio with one molecule per actin subunit
in muscle and nonmuscle cells from plants and animals.
It is suitable for labeling and identifying F-actin in tissue
sections or cell cultures.
-20°C, protect from light
CellTracker™ Green Fluorescent Probe

(5-chloromethylfluorescein diacetate)
CellTracker™ Green readily crosses the plasma membrane
of intact cells and is retained intracellularly after reacting
with cytoplasmic thiols. Cell labeling is stable for 24 hours
or more in most cell types, making CellTracker™ Green well
suited to applications requiring a long-lasting fluorescent
marker. Human skeletal muscle myoblasts fixed and stained with Phalloidin,
Alexa Fluor® 488 and DAPI
CellTracker™ Orange Fluorescent Probe

(5-(and-6)-(((4-chloromethyl)benzoyl)amino) tetramethylrhodamine )
CellTracker™ Orange is similar to CellTracker™ Green but
with longer wavelength absorption and emission maxima.
Many cell types loaded with the CellTracker™ Probes are
both brightly fluorescent and viable for at least 24 hours
after loading, and labeling can often persist through several
cell divisions.
-20°C, protect from light Normal human astrocytes stained live with MitoTracker® Red
MitoTracker® Red CMXRos Mitochondrial Probe

MitoTracker® Red is a cell-permeant fluorescent probe
that accumulates in active mitochondria. It labels living
cells at submicromolar concentrations and is retained in
mitochondria following aldehyde-based fixation and cell
permeabilization, thus allowing subsequent staining with
antibodies or other non-permeant fluorescent probes.
242
Continued
DAPI Nucleic Acid Stain Ordering Information

The blue fluorescent DAPI Nucleic Acid Stain preferentially Cat. No. Size Price
stains dsDNA. In addition, DAPI binds to RNA and exhibits
a longer-wavelength fluorescence emission maximum DAPI Nucleic Acid Stain
than DNA binding. DAPI has limited cell permeability and PA-3013 10 mg
therefore is typically used as a nuclear counterstain with
multicolor fluorescent labeling of fixed cells. Hoechst 33342 Nuclear Counterstain
■ Storage Conditions: PA-3014 10 ml

Room temperature, protect from light LysoTracker® Red Lysosomal Probe
PA-3015 5 × 50 µl
Hoechst 33342 Nuclear Counterstain
Live / Dead® Viability / Cytotoxicity Assay Kit
Hoechst is a cell-permeant nuclear counterstain that emits PA-3016 Kit
blue fluorescence when bound to dsDNA. It is often used
to distinguish condensed pycnotic nuclei in apoptotic cells,
and used for cell cycle studies in combination with BrdU.
4°C – 8°C, protect from light
LysoTracker® Red Lysosomal Probe
LysoTracker® Red is an acidotropic probe useful for selective

fluorescent labeling and tracking of acidic organelles in
live cells. It is highly cell-permeant and labels living cells
at nanomolar concentrations.
Hoechst 33342 Nuclear Counterstain in live microvascular
endothelial cells
Live / Dead® Viability / Cytotoxicity Assay Kit
This two-color, fluorescence cell viability assay kit allows

for the simultaneous determination of live and dead
eukaryotic cells. Calcein AM is cleaved by intracellular
esterases to brightly label live cells with green
fluorescence. Ethidium homodimer (EthD-1) enters cells
that have lost plasma membrane integrity, producing
bright red fluorescent labeling of dead cells. The kit is
suitable for use with fluorescence microscopes or multi-
well plate scanners and easily adaptable for use with flow
cytometers or other fluorescence detection systems.
-20°C, protect from light Human skeletal muscle myoblasts stained with LysoTracker® Red
and SYTO® Green.
243
Continued
SYTO® Green 11 Nucleic Acid Stain Ordering Information

SYTO® Green is a cell-permeant nucleic acid stain useful for Cat. No. Size Price
visualization of DNA and RNA in both live and fixed cells.
It shows a large fluorescent enhancement upon binding SYTO® Green 11 Nucleic Acid Stain
nucleic acids, and may be used with eukaryotic cells and PA-3018 250 µl
Gram-positive and Gram-negative bacteria. Eukaryotic
cells labeled with SYTO® Green will typically exhibit bright SYTOX® Green Nucleic Acid Stain
nuclear staining as well as diffuse cytoplasmic staining. PA-3019 250 µl
Vybrant® DiI Cell-Labeling Solution
PA-3020 1 ml
SYTOX® Green Nucleic Acid Stain Annexin V, Alexa Fluor® 488 Conjugate
SYTOX® Green is chemically similar to SYTO® Green but is PA-3021 125 µl (25 assays)
not cell permeant. SYTOX® Green may be used as a green
nuclear counterstain for multicolor fluorescence of fixed
cells or as a dead cell indicator in viability assays.
Vybrant® DiI Cell-Labeling Solution Cell analysis reagent excitation/emmission specifications

DiI is a lipophilic tracer useful for labeling the membranes Cat. No. Cell Type Excitation/Emission (nm)
of living cells. Vybrant® DiI is an easy-to-use cell-labeling PA-3010 Phallodin, Alexa Fluor® 488 Conjugate 495/519
solution that can be added directly to aqueous culture PA-3011 CellTracker™ Green Fluorescent Probe 492/517
media to uniformly label the membranes of attached or PA-3012 CellTracker™ Orange Fluorescent Probe 541/565
suspended cells. PA-3013 DAPI Nucleic Acid Stain 358/461
■ Storage Conditions PA-3014 Hoechst 33342 Nuclear Counterstain 350/461
-20°C, protect from light PA-3015 LysoTracker® Red Lysosomal Probe 590/617
PA-3016 LIVE/DEAD® Viability/Cytotoxicity Assay Kit 494/517
Annexin V, Alexa Fluor ® 488 Conjugate 528/617
PA-3017 MitoTracker® Red CMXRos Mitochondrial Probe 579/599
Annexin V conjugates bind to phosphatidylserine (PS) on PA-3018 SYTO® 11 Green Nucleic Acid Stain 499/522
apoptotic cell surfaces in the presence of Ca 2+. It can also PA-3019 SYTOX® Green Nucleic Acid Stain 504/511
pass through the compromised membranes of necrotic
PA-3020 Vybrant® Dil Cell-Labeling Solution 549/565
cells and bind to PS in the interior of the cell. Annexin V,
Alexa Fluor® 488 is useful for identifying apoptotic cells by PA-3021 Annexin V, Alexa Fluor® 488 Conjugate 495/519
flow cytometry or fluorescence microscopy.
4°C – 8°C, protect from light
Differentiated NHNP stained live with Vybrant® Dil (red) and SYTO® Differentiated human skeletal muscle myoblasts stained with SYTOX®
Green Green
244
G Protein-Coupled Receptors
Quality, quantity, quickly
G protein-coupled receptors (GPCRs) are a large super- sense that it is not known what endogenous molecules
family of cell-surface proteins whose function is to activate these receptors). They represent a tremendous
transduce information from extracellular space to opportunity to uncover novel ligands and novel therapeutic
the cell interior by stimulating (or inhibiting) second approaches to major diseases.
messenger systems. Included in this family are receptors
for neurotransmitters (such as receptors for dopamine, In the past few years, a new paradigm for drug discovery
acetylcholine, histamine, etc.) as well as receptors for has been elucidated, the “reverse pharmacological”
brain and gut peptides (e.g. bradykinin, gallanin, and approach to drug discovery. In a “normal” drug discovery
various neuropeptides). project, a target would be identified and validated as a
G Protein-Coupled
proper target for a particular therapeutic intervention,
GPCRs are an extremely important superfamily comprised then a screening program would be constructed around
Receptors
of some 800 different members. Drugs currently on the that target. In a reverse pharmacological approach, the
market that interact with GPCRs interact with only about screening of an orphan receptor precedes the target’s
100 out of the 800 identified GPCRs. Approximately 30% validation for a particular therapeutic intervention.
of all pharmaceuticals on the market today exert their
therapeutic effect by interacting with a GPCR. GPCRs still Many orphan GPCRs have been characterized in the past
represent untapped potential for drug discovery. few years by the discovery of the endogenous substances
which activate these receptors. The remaining orphan
Of these 800 genes, approximately 400 represent olfactory GPCRs are likely to contribute additional targets to these
receptors, and approximately 50 represent pseudogenes. and other disease areas.
Of the remaining 340 or so genes, approximately 200
represent known receptors and 140 represent unknown We are pleased to present this GPCR offering and look
or orphan receptors. Orphan receptors are receptors that forward to announcing new GPCRs to you routinely.
are known to be part of the GPCR family by virtue of their
molecular sequence but whose biology is unknown (in the
Storage at -80°C is recommended, repeated freeze-
thawing of this product is not recommended
Representative GPCR characterization data
7,500
GTPG 35S Bound (cpm)
5,000
For more information concerning licensing opportunities, please call

301-898-7025, ext. 7350.
2,500
0
-10 -8 -6 -4
Additional available information through the web site,
Carbachol (M) www.lonza.com/research, or Scientific Support at
Recombinant human M1 muscarinic receptor. 1-800-521-0390
A full listing of cell lines and membranes offered
Your Sales Representative contact information and quote request
General recommended assay conditions
Host cell
Protein concentration
GenBank accession number
EC50
Binding assay data lot specific
245
Bioservices
Combining cell culture expertise with innovative bioassays and development capabilities
Bioassays are a critical part of the drug development Cytotoxicity and Apoptosis BioAssays
pipeline, from discovery through clinical trials to lot
Lonza Bioservices offers a number of bioassays to
release. The diverse skills of our Bioservices group make
address cell viability, cytotoxicity, apoptosis, and
us an excellent partner, whatever your bioassay needs,
proliferation in culture to determine test compound
whether for research and development purposes, or per
toxicity in the drug discovery process. These bioassays
cGMP or cGLP guidelines. We combine client-specific
take advantage of the power of ATP bioluminescent
project management with rapid and flexible turnaround
detection for exceptional sensitivity, reproducibility, and
time to meet your requirements. Bioservices specializes
rapid turnaround.
in the development, qualification, and validation of cell-
based bioassays. We apply the same world class expertise — Exceptionally sensitive with a detection limit of as few
Bioservices
used in development of more than a thousand cell culture as 10 cells per well
products. No one knows more about cells than we do. — Highly reproducible CV’s of <5%
— Effective tests for adherent and suspension
BioAssay Method Development
mammalian cells as well as bacteria and yeast
We have developed a wide range of bioassays to — Apoptosis bioassays correlate well with established
characterize biological and biopharmaceutical products. apoptosis assays, including TUNEL, propidium iodide,
Our capabilities include, but are not limited to: Annexin V, and caspase assays
Cell-Based BioAssays Biological Systems Analyzed
— Apoptosis — Adipogenesis
— Differentiation — Angiogenesis
— Proliferation — Hematopoiesis Typical cytotoxicity results for several drug compounds
using hepatocytes and hematopoietic progenitors
— Purity — Neurology
— Cytotoxicity — Osteogenesis Primary Human Hepatocytes
Luminescence (RLU)
— Cytokine induction — Immunology 14,000
12,000
— Necrosis
10,000
— Neutralization
8,000
BioAssay Qualification and Validation 6,000 Toxic Control

Compound 1
We will qualify your bioassay and then validate the 4,000 Compound 2
Compound 3
bioassay’s parameters, including accuracy, precision, 2,000 Compound 4
specificity, linearity and robustness, in accordance with
regulatory guidelines. 0
.0005 .005 .05 .5 1 5 10
Concentration (µM)
BioAssay Performance Primary Human CD34+ Cells

We perform validated, stability-indicating bioassays 100,000
Luminescence (RLU)
and potency bioassays to meet your regulatory

requirements.
10,000
Contact our Bioservices Team for detailed discussions of
your needs.
Toxic Control
Phone: 800-654-4452, ext.2375 1,000
Compound 1
Compound 2
301-898-7025, ext. 2375 Compound 3
Compound 4
Fax: 301-845-0165
E-mail: ProcessDevelopment@lonza.com 100 .005 .05 .1 .5 1 10 100
Concentration (µM)
246
BioAssays FAQs
MycoAlert® Mycoplasma Detection Kit ViaLight® Cell Proliferation and Cytotoxicity Assays
Q. Does the kit recognize the whole spectrum of Q. How does the performance of the ViaLight®
mycoplasma? Assays compare with other assays routinely
used in cell proliferation/cytotoxicity assays?
A. The mycoplasma specific metabolism detected
by the MycoAlert® Kit is present in all members A. The combination of extreme sensitivity and wide
of the mollicutes family. 96% of all mycoplasma dynamic range of bioluminescent measurement
infections of cells grown in culture are caused allows the ViaLight® Assays to determine very
by just six species. The MycoAlert® Kit can subtle changes in the viability status of cultures
detect these species and many more; the list which reveal cytotoxicity missed by other
is ongoing. Please contact Scientific Support for
BioAssay FAQs
methods such as tetrazolium dyes, resazurin
further information. reagents and LDH assays.
Q. What type of sample do I need? Q. What is the difference between the ViaLight®
Can it be stored? HS and ViaLight® Plus Cell Proliferation and
A. The MycoAlert® Kit requires the use of cell free Cytotoxicity BioAssay Kits?
supernatant only! Ideally you should sample A. ViaLight® HS Assay can detect less than 10
your cell cultures and test immediately. If mammalian cells per microwell and measures
needed you can store the supernatant at room over a dynamic range of 5 decades. The ViaLight®
temperature for 8 hours, 2°C – 8°C for 1 week Plus Assay is designed to give enhanced signal
or 2 months at -20°C. (Thaw sample without stability and light output – signal half-life is in
the aid of artificial heat and equilibrate to room excess of 5 hours with greater reagent stability
temperature before use in assay). with the same sensitivity of the ViaLight® HS
Q. What about a positive control? Assay.
A. The MycoAlert® Assay Control Set (LT07-518) is
available separately and includes a lyophilized Continued on next page
positive control (1 ml) and assay buffer (2
ml) for reconstitution. The assay buffer also
serves as a negative control. The positive and
negative controls are designed for use with the
MycoAlert® Mycoplasma Detection Kit for the
detection of mycoplasma contaminations in cell
cultures. The Assay Control Set may be used to
ensure that the operator and all of the assay
reagents are performing properly.
The positive control is proprietary, but does NOT
contain mycoplasma. The positive or negative
control is simply substituted for a sample in the
standard assay protocol. The positive control
will give a ratio of >1 while the negative control
will give a ratio <1.
247
BioAssays FAQs
Continued
ToxiLight® Non-destructive Cytotoxicity BioAssay ApoGlow® Rapid Apoptosis Screening Assay
Q. Can the ToxiLight® Assay be used for all cell Q. How does the ApoGlow® Assay differentiate
types? between apoptosis and necrosis?
A. Yes, the ToxiLight® Kit is intended for use with all A. Interpretation of the results is based on the
mammalian cell types, with both adherent and ratio of ATP to ADP. The interpretation of different
non-adherent cells. As adenylate kinase (AK) is ratios obtained, within each experimental
ubiquitous, the principle can also be applied to situation, may vary according to the cell types
bacterial, fungal and plant cells. and conditions used. However, in general terms,
if a test gives considerably lower ATP levels than
Q. How does the ToxiLight® Kit compare with
BioAssay FAQs
the control, but greatly increased ADP level, it

conventional assays for the determination of would indicate necrosis. Whereas, if the test
cytolysis? gives slightly lower levels of ATP to control,
but shows an increase in ADP, it would point to
A. The ToxiLight® Kit compares extremely favorably apoptosis. (These ratios vary according to the
with all other routinely used assays including degree of apoptosis in the cell population). It
lactate dehydrogenase (LDH), chromium is the relationship between the ratios and the
staining and the nucleic acid stain propidium control that is the key to the indication of the
iodide. LDH is measured using a colorimetric mode of cell death. See ApoGlow® Kit insert or
substrate; disadvantages include poor dynamic contact Scientific Support for further guidance.
range, a lack of sensitivity, interference by
cellular debris and long pre-incubation periods Q. How do ApoGlow® Assay test results correlate
prior to measurement. 51Chromium release uses with other apoptosis assay methods?
radioisotopes and as such is in rapid decline.
A. The ApoGlow® Assay correlates very well with
Q. Can I measure the kinetics of my cytolysis other methods of cell death identification,
studies? including flow cytometric analysis using
propidium iodide and TUNEL, giving significant
A. Yes, as the ToxiLight®Kit is a non-destructive improvements in reproducibility.
assay, repeated samples of supernatant can
be collected at different points throughout the Q. What is the principle behind the ApoGlow®
course of an experiment without disrupting the Assay?
cells themselves. This enables the kinetic study
of cell death. A. Changes in the ratio of adenylate nucleotides, ADP
and ATP, can be used to detect and measure
Q. What are the differences between ToxiLight® apoptosis, necrosis and cell proliferation.
and ViaLight® Kits? Apoptosis is an energy driven process, and
as such, ATP is required for many of the early
A. The ToxiLight® Kit is a method of quantifying events of apoptosis. As the process continues,
the lysis of the cell with the enzyme adenylate ATP levels will eventually drop to a level
kinase in the terminal stages of cell death, where the cell can no longer perform basic
whereas the ViaLight® Kits measures ATP levels functions and the cell will die. The ApoGlow®
and therefore the number of metabolically active Assay uses bioluminescence catalyzed by the
cells. firefly enzyme luciferase to measure these
intracellular nucleotides in a stepwise reaction,
to give a sensitive and rapid detection of cell
death/proliferation. Through extensive research,
we have demonstrated that a change in the
ratio of ADP to ATP occurs when mitochondrial
function becomes compromised in apoptosis,
and that specifically cells in the early stages
of apoptosis show an increase in ADP without
an appreciable drop in ATP. Bioluminescent
detection of adenylate nucleotides is very
simple and extremely reproducible.
248
BioWhittaker® Cell Culture
Chapter 5
BioWhittaker® Cell Culture
BioWhittaker®
Cell Culture
Classical Media Cell Culture Technical Information

Introduction 251 Adaptation of Cell Cultures to Serum-free Medium 298
BioWhittaker® Classical Media 253 Protocols for Weaning Cell Cultures 299
Cryopreservation and Reconstitution 300
Specialty Media Determination of Cell Numbers 301
Powdered Medium Preparation 303
Introduction 261
Subculturing Procedures for Mammalian Cells 304
General Purpose Serum-free Media 262
Media FAQs 306
X-VIVO™ Chemically Defined, Serum-free
Sera FAQs 307
Hematopoietic Cell Media 265
Cell Culture Reagents FAQs 308
CHO Media 266
Formulations for BioWhittaker® Classic Media 309
NAO Freezing Medium 269
Renal Media 269
Specialty Media - IVF 272
Specialty Media - Cytogenetics 273 New Products
Hybridoma Media 274
Insect-XPRESS™ Protein-free Insect Cell Medium 278 PowerCHO® -GS Chemically Defined,
ProNS0™ Chemically Defined, Protein-free Media 279 Serum-free CHO Medium 268

Introduction 281
Reagents 283

Introduction 289
Equine Sera 294
Bovine Sera 295
Human Sera 295
Fetal Bovine Sera
For further manufacturing or laboratory use. Not for use in diagnostic procedures.
250
BioWhittaker® Classical Media
Classical Media
BioWhittaker®
Liquid Cell Culture Media
Chemically defined liquid media are used to provide Chemicals used to prepare liquid media products are
nutrients for cell culture growth in research, diagnostic, purchased according to the raw material qualifications
and manufacturing applications. In order to meet the from approved suppliers. Each lot must meet established
diverse needs of the markets we serve, the production component specifications before it is released by Quality
and quality control procedures for liquid media emphasize Assurance for use. We manufacture all liquid cell culture
control. Our goal is to provide customers with high quality media using Water for Injection (WFI) quality water, which
products that are consistent from batch to batch. With all has been prepared by ultrafiltration, reverse osmosis,
of the time that you take to optimize your systems, we want deionization, and distillation. Liquid media is sterile filtered
to provide you with products you can trust. through pharmaceutical-grade sterilizing filters. The for-
mulations used for standard liquid media products are
All BioWhittaker® Classical Media products produced are those recommended by the Tissue Culture Association.
manufactured in accordance with cGMP regulations. A continued on next page
Device Master Record (DMR) is prepared for every liquid
medium product. It defines the procedures for production
from receipt of raw materials to final product release,
the environmental and processing controls required,
and product specifications, including packaging and
labeling. Product manufacturing is consistent with the
requirements defined in the DMR, which ensures that
each lot of a product is consistent with all other lots of the
same product.
251
Continued
Classical Media
BioWhittaker®
Quality Control
The combined result of all in-process monitoring and final
In order to maintain consistent quality in sterile cell culture
product testing is the further assurance that each lot has
media products, strict quality control of each production lot
been prepared not only according to approved written
is essential. Written procedures in accordance with current
procedures, but has passed test criteria, and will meet
Good Manufacturing Practices (cGMPs) provide quality
design specifications. Samples from each lot of standard
control from start to finish for each product produced.
product are retained and stored at label temperature in
Final product testing includes the following:
the event follow-up testing is required. Subsequent testing
may be in response to customer inquiries or for shelf life
USP Sterility – Tests are performed on representative samples studies.
using the membrane filtration procedure in accordance
with the US Pharmacopoeia or EP Pharmacopoeia. Culture Micronized Media (Powder)
media used are fluid thioglycollate medium (FTM) and
trypticase soy broth (TSB). All test media must pass Some tissue culturists prefer to prepare their own liquid
growth promotion testing before use. The test samples are medium from powder. Therefore, we are committed to
filtered and the filters are immersed in FTM and TSB. TSB offering you the same high quality powdered media as our
cultures are incubated at 22.5°C ± 2.5°C. FTM cultures are customers have come to expect with our liquid media. We
incubated at 32.5°C ± 2.5°C. The cultures are incubated for utilize unique manufacturing processes, which produce
14 days, during which they are periodically examined and the powder in a controlled environment, guaranteeing
sterility results are recorded. maximum stability and minimum degradation due to
chemical oxidation. Standard package sizes are 10 L
Chemistries (pH and osmolality) – Tests are performed and 50 L.
on representative samples from each lot using routinely
calibrated equipment. Osmolality is determined by means Quality Control Testing
of the highly-repeatable freezing point method. All powdered media are subject to rigorous quality control
testing. These tests include: pH, osmolality, cell growth,
Cell Growth Promotion – Media and supplements are endotoxin, moisture content, and a sieve test.
tested for their growth promoting properties using
primary, diploid, and heteroploid cell lines unless other Shelf Life
cells are appropriate based on the product use. Results Powdered media are generally very stable at refrigerated
are obtained using the Lowry protein method or manual temperatures. Shelf life is two years on most powdered
cell count to assess cell replication. Visual screening products.
of cultures is also done to ensure characteristic cell
For information about how to prepare powdered media, please refer to the
morphology and lack of toxicity. Technical Information section at the end of this chapter, page 303
Additional testing for toxicity, function and/or activity may

also be conducted if one or more is required in the product
release specifications.
Endotoxin – Products are tested for endotoxin content

using the Kinetic-QCL® Assay. Test dilutions used
are screened for inhibition and enhancement in the
Kinetic-QCL® Assay. Endotoxin levels for these products
are available on Certificates of Analysis.
252
Continued
Classical Media
BioWhittaker®
Basal Medium Eagle (BME) Cat. No. Size Price
A minimal medium suitable for a variety of cell types. It is a historical precursor
to Minimum Essential Medium (MEM).
Dulbecco’s Modified Eagle’s Medium
with 4.5 g/L glucose, without L-glutamine
Storage Conditions: 2°C – 8°C
Cat. No. Size Price
12-614F 500 ml
Cryoprotective Medium 12-604F/12 12 x 500 ml
without L-glutamine, with 15% DMSO (Use 1:1 with growth medium)
12-614Q 1L
12-604Q/12 12 x 1 L
12-132A 100 ml
Dulbecco’s Modified Eagle’s Medium, Powder
See also ProFreeze™-CDM, Non-Animal Origin, Chemically Defined Freeze Medium, with 4.5 g/L glucose, without L-glutamine or sodium pyruvate
Cat. No. 12-769E, page269. Storage Conditions: 2°C – 8°C
See page 300 for a basic procedure for cryopreservation of cells.
15-614D 1 × 10 L
Basal Medium Eagle with Earle’s BSS
without L-glutamine Dulbecco’s Modified Eagle’s Medium
Storage Conditions: 15°C – 30°C with 4.5 g/L glucose, with L-glutamine
12-105F 500 ml
12-604F 500 ml
12-604F/12 12 x 500 ml
Dulbecco’s Modified Eagle’s Medium (DMEM) 12-604Q 1L
12-604Q/12 12 x 1 L
DMEM is used in a wide range of mammalian cell culture applications. The
high glucose version is well suited to high density suspension culture. The
low glucose formula is used for adherent dependent cells. Dulbecco’s Modified Eagle’s Medium
with 4.5 g /L glucose, with UltraGlutamine I
BE12-604F/U1 500 ml
Dulbecco’s Modified Eagle’s Medium, Powder

with 4.5 g/L glucose, L-glutamine, without sodium pyruvate
15-604D 1 × 10 L
Without sodium pyruvate
Hybridoma screened
With UltraGlutamine I
With 1.0 g/L glucose
With 4.5 g/L glucose
Without L-glutamine
BE15-604F 1 × 50 L
Without phenol red
With L-glutamine

With HEPES
with 4.5 g/L glucose, without L-glutamine (hybridoma screened)

Powder

Cat. No. Size
12-614F 500 ml 12-914F 500 ml
■ ■
12-614Q 1L
15-614D 1 ✕ 10 L ■ ■ ■ ■ Dulbecco’s Modified Eagle’s Medium

with 4.5 g/L glucose, without L-glutamine or phenol red
12-604F 500 ml Storage Conditions: 2°C – 8°C
■ ■
12-604Q 1L
12-917F 500 ml
BE12-604F/U1 500 ml ■ ■
15-604D 1 ✕ 10 L
■ ■ ■ ■
BE15-604F 1 ✕ 50 L
12-914F 500 ml ■ ■ ■
12-917F 500 ml ■ ■ ■
12-707F 500 ml ■ ■
12-708F 500 ml ■ ■ ■
12-709F 500 ml ■ ■ ■
12-733F 500 ml
■ ■ ■
12-733Q 1L
12-741F 500 ml ■ ■ ■
Please see the Product Formulations section for exact compositions.

253
Continued
Classical Media
BioWhittaker®
Ordering Information DMEM:F12 Medium

DMEM:F12 combines the richness of F12 with the higher component
Cat. No. Size Price concentrations of DMEM. This media is well suited for clonal density
cultures.
with 1.0 g/L glucose, without L-glutamine
With L-glutamine
12-707F 500 ml
Without HEPES
With HEPES
with 1.0 g/L glucose and 25 mM HEPES Buffer, without L-glutamine
Storage Conditions: 2°C – 8°C Cat. No. Size
12-719F 500 ml
■ ■
12-708F 500 ml 12-719Q 1L
BE04-687Q 1L ■ ■
with 4.5 g/L glucose and 25 mM HEPES Buffer, without L-glutamine BE04-687F/U1 500 ml ■ ■
12-709F 500 ml
Ordering Information
with 4.5 g/L glucose, without L-glutamine or sodium pyruvate Cat. No. Size Price
DMEM:F-12, 1:1 Mixture
12-733F 500 ml with 3.151 g/L glucose, L-glutamine, and 15 mM HEPES Buffer
12-733Q 1L
12-719F 500 ml
with 4.5 g/L glucose, with L-glutamine, without sodium pyruvate 12-719Q 1L
DMEM:F-12 1:1 Mixture
12-741F 500 ml with 3.151 g/L glucose, L-glutamine, without HEPES
BE04-687Q 1L
DMEM:F12 1:1 Mixture

with 3.151 g /L glucose, with UltraGlutamine I, without HEPES
BE04-687F/U1 500 ml
254
Continued
Classical Media
BioWhittaker®
Glasgow Minimum Essential Medium (GMEM) Iscove’s Modified Dulbecco’s Medium (IMDM)
Designed to support BHK-21 cells. IMDM is suitable for fast growing cells. All formulas contain HEPES for added
buffering.
Cat. No. Size Price
Hybridoma screened
Without L-glutamine
Glasgow Minimum Essential Medium
With L-glutamine
(G-MEM, BHK-21)
With HEPES
12-739F 500 ml Cat. No. Size

12-722F 500 ml
Grace’s Insect Medium 12-722Q 1L
■ ■
12-726F 500 ml
Cat. No. Size Price ■ ■
12-726Q 1L
Grace’s Insect Medium 12-915F 500 ml ■ ■ ■
without lactalbumin hydrolysate, yeastolate, gentamicin or FBS
Storage Conditions: 2°C – 8°C Please see the Product Formulations section for exact compositions.
04-457F 500 ml Ordering Information

Grace’s Complete Insect Medium Cat. No. Size Price
(TNM-FH)
with 10% heat-inactivated FBS, yeastolate, lactalbumin hydrolysate, and Iscove’s Modified Dulbecco’s Medium
gentamicin with 25 mM HEPES Buffer and L-glutamine
Storage Conditions: 2°C – 8°C Storage Conditions: 2°C – 8°C
04-501Q 1L 12-722F 500 ml

12-722Q 1L
Grace’s Insect Medium
with lactalbumin hydrolysate, yeastolate, and gentamicin, without FBS
Storage Conditions: 2°C – 8°C Iscove’s Modified Dulbecco’s Medium
with 25 mM HEPES Buffer, without L-glutamine
04-649F 500 ml
12-726F 500 ml
12-726F/12 12 x 500 ml
12-726Q 1L
Ham’s Medium
12-726Q/12 12 x 1 L
Developed for low density (clonal) growth of CHO cells.
Iscove’s Modified Dulbecco’s Medium
Cat. No. Size Price with 25 mM HEPES Buffer and L-glutamine (hybridoma screened)
Ham’s F10 Medium
with L-glutamine 12-915F 500 ml
12-618F 500 ml
Ham’s F10 Medium

with UltraGlutamine I, without thymidine
BE02-014F 500 ml
Ham’s F12 Medium

with L-glutamine
12-615F 500 ml
255
Continued
Classical Media
BioWhittaker®
L-15 (Leibovitz) Medium McCoy’s 5A Medium

Developed for fast growing tumor cells, this formula does not require a Designed for human lymphocyte culture.
CO2 enriched atmosphere. The bicarbonate-free medium is buffered with
elevated levels of amino acids. Ordering Information
Ordering Information Cat. No. Size Price
Cat. No. Size Price McCoy’s 5A Medium

with L-glutamine
L-15 (Leibovitz) Medium Storage Conditions: 2°C – 8°C
without L-glutamine
Storage Conditions: 2°C – 8°C 12-688F 500 ml
12-700F 500 ml McCoy’s 5A Medium

12-700Q 1L with L-glutamine and 25 mM HEPES Buffer
L-15 (Leibovitz) Modified Medium (2X) 12-168F 500 ml
(2X) except L-tyrosine (1X), without L-glutamine or phenol red (virus
plaquing medium)
12-669E 100 ml
Minimum Essential Medium - Eagle (MEM Eagle) (E-MEM)

Minimum Essential Medium (E-MEM) is suitable for a diverse spectrum of mammalian cell types. It is available with either Hanks' or Earle’s salts. MEM-Hanks’
(12-127F or 12-137F) does not require a CO2 enriched atmosphere. Joklik’s modification is intended for suspension culture.
amphotericin B, gentamicin, FBS

With penicillin, streptomycin,
With D-valine, not L-valine

Without Na-Bicarbonate
With sodium pyruvate
Without L-glutamine
Without phenol red
With nucleosides
With L-glutamine
Without calcium
With HEPES
With HBSS
With NEAA
With EBSS
Powder
Cat. No. Size
12-169F 500 ml ■ ■ ■
BE02-002F 500 ml ■ ■ ■ ■
12-611F 500 ml
■ ■
12-611Q 1L
15-611D 1 ✕ 10 L ■ ■ ■ ■
12-125F 500 ml
■ ■
12-125Q 1L
12-662F 500 ml
■ ■ ■ ■
12-662Q 1L
12-136F 500 ml
■ ■ ■
12-136Q 1L
12-736E 100 ml
■ ■ ■ ■ ■
12-736F 500 ml
12-684F (10X) 500 ml ■ ■ ■
12-668E (2X) 100 ml ■ ■ ■
06-174G 450 ml ■ ■ ■ ■
BE02-020F 500 ml ■ ■ ■
12-127F 500 ml ■ ■
12-137F 500 ml ■ ■ ■
04-719Q (Joklik’s) 1L ■ ■

256
Continued
Classical Media
BioWhittaker®
MEM Alpha Eagle with Earle's BSS MEM Eagle with Earle’s BSS
without L-glutamine, deoxyribonucleosides or ribonucleosides Cell culture maintenance medium 12-136 with NEAA, L-glutamine, 25
Storage Conditions: 2°C – 8°C mM HEPES buffer, 10 µg/ml gentamicin, 50 units/ml penicillin, 50 µg/ml
streptomycin, 2.5 µg/ml amphotericin B, and 2.0% heat-inactivated FBS
MEM Alpha Eagle 12-736E 100 ml

with ribonucleosides and deoxyribonucleosides, with UltraGlutamine I 12-736F 500 ml
BE02-002F 500 ml
MEM Eagle with Earle’s BSS (10X)
without NaHCO3 or L-glutamine
MEM Eagle with Earle’s BSS
with L-glutamine 12-684F 500 ml
12-611F 500 ml
MEM Eagle with Earle’s BSS (2X)
without L-glutamine or phenol red (virus plaquing medium)
12-611F/12 12 x 500 ml Storage Conditions: 15°C – 30°C
12-611Q 1L 12-668E 100 ml
12-611Q/12 12 x 1 L BE12-668F 500 ml
MEM Eagle with Earle’s BSS (Powder) MEM Eagle with Earle’s BSS
with L-glutamine with non-essential amino acids and L-glutamine, without calcium
15-611D 1 × 10 L 06-174G 450 ml
MEM Eagle with Earle’s BSS MEM Eagle with Earle’s BSS D-Val Medium
without L-glutamine without L-glutamine, with D-valine substituted for L-valine, use dialyzed serum
Storage Conditions: 15°C – 30°C (14-810F) to supplement
12-125F 500 ml
12-125F/12 12 x 500 ml BE02-020F 500 ml
12-125Q 1L
MEM Eagle with Hanks’ BSS
12-125Q/12 12 x 1 L without L-glutamine
with non-essential amino acids and sodium pyruvate, without L-glutamine 12-127F 500 ml
MEM Eagle with Hanks’ BSS
12-662F 500 ml with 25 mM HEPES Buffer, without L-glutamine
12-662Q 1L Storage Conditions: 15°C – 30°C
12-137F 500 ml
with 25 mM HEPES Buffer, without L-glutamine
Storage Conditions: 15°C – 30°C MEM Eagle Joklik’s Formulation
for suspension cultures, with L-glutamine
12-136Q 1L 04-719Q 1L
257
Continued
Classical Media
BioWhittaker®
Medium 199 NCTC-109 Medium

Medium 199 was originally formulated for chick embryo fibroblast culture. A complex formula used to supplement hybridoma medium.
These four formulations require a CO2 enriched atmosphere.
Cat. No. Size Price
NCTC-109 with Earle’s BSS
With 1.4 g/L Na-bicarbonate

with L-glutamine (hybridoma screened)
12-923E 100 ml
With L-glutamine
With HEPES
With HBSS
With EBSS
Richter’s CM Medium
Cat. No. Size Also known as I-MEM – Improved MEM with zinc.
12-109F 500 ml ■ ■ ■ Cat. No. Size Price
12-117F 500 ml
12-117Q 1L
■ ■ ■ Richter’s CM Medium
with L-glutamine
12-118F 500 ml ■ ■ ■ ■ Storage Conditions: 2°C – 8°C
12-119F 500 ml ■ ■ 04-648F 500 ml
Please see the Product Formulations section for exact compositions. RPMI 1640 Medium
RPMI is a general purpose media with a broad range of applications
Ordering Information for mammalian cells, especially hematopoietic cells. RPMI with MOPS
(04-525F) is for certain mycological assays.
Cat. No. Size Price
Medium 199 with Hanks’ BSS

with L-glutamine and 1.4 g/L NaHCO3
With penicillin – streptomycin

Without Na-bicarbonate
12-109F 500 ml
Without L-glutamine
Without phenol red
Without D-glucose
With MOPS buffer
With L-glutamine
Medium 199 with Earle’s BSS
with L-glutamine, 25 mM HEPES Buffer, and 2.2 g/L NaHCO3
With HEPES
Powder
12-117F 500 ml Cat. No. Size
12-117Q 1L 12-702F 500 ml

12-702Q 1L ■
Medium 199 with Hanks’ BSS BE12-702F/U1 500 ml ■

with L-glutamine, 25 mM HEPES Buffer, and 1.4 g/L NaHCO3
15-702D 1 ✕ 10 L ■ ■ ■
12-167F 500 ml
12-118F 500 ml 12-167Q 1L ■
08-012B 10 L ■
Medium 199 with Earle’s BSS
with L-glutamine and 2.2 g/L NaHCO3 BE12-115E 100 ml
Storage Conditions: 2°C – 8°C 12-115F 500 ml ■ ■
12-115Q 1L
12-119F 500 ml
BE12-115F/U1 500 ml ■ ■
04-525F 500 ml ■ ■ ■
09-774E 100 ml
■ ■ ■
09-774F 500 ml
12-918F 500 ml ■ ■
BE12-752F 500 ml ■ ■
258
Continued
Classical Media
BioWhittaker®
RPMI 1640 RPMI 1640 Medium

with L-glutamine with L-glutamine, 25 mM HEPES Buffer, 100 units/ml penicillin, and
Storage Conditions: 2°C – 8°C 50 µg/ml streptomycin
12-702F 500 ml
12-702F/12 12 x 500 ml 09-774E 100 ml
12-702Q 1L 09-774F 500 ml
12-702F/12 12 x 1 L
RPMI 1640 Medium
RPMI 1640 Medium without L-glutamine or phenol red
with UltraGlutamine I Storage Conditions: 15°C – 30°C
12-918F 500 ml
BE12-702F/U1 500 ml
RPMI 1640 Medium
with L-glutamine, without D-glucose
RPMI 1640 (Powder) Storage Conditions: 2°C – 8°C
with L-glutamine
BE12-752F 500 ml
BE15-702D 1 × 10 L
Schneiders' Drosophila Medium
RPMI 1640
without L-glutamine Cat. No. Size Price
Schneiders' Drosophila Medium, Modified
12-167F 500 ml with L-glutamine
12-167F/12 12 x 500 ml Storage Conditions: 2°C – 8°C
12-167F/24 24 500 ml 04-351Q 1L
12-167Q 1L
12-167Q/12 12 x 1 L TC 100 Insect Medium
08-012B 10 L
Cat. No. Size Price
10 L size is provided in a flexible bag with one female and one male luer
connector.
TC 100 Insect Medium
with L-glutamine
RPMI 1640 Storage Conditions: 2°C – 8°C
with L-glutamine and 25 mM HEPES Buffer
Storage Conditions: 2°C – 8°C BE02-011F 500 ml
BE12-115E 100 ml Waymouth's MB 752/1 Medium

12-115F 500 ml
12-115F/12 12 x 500 ml Cat. No. Size Price
12-115Q 1L Waymouth's MB 752 /1 Medium
12-115Q/12 12 x 1 L with L-glutamine
RPMI 1640 Medium BE12-738F 500 ml
with UltraGlutamine I and 25 mM HEPES
Williams' Medium E
BE12-115F/U1 500 ml
Cat. No. Size Price
RPMI 1640 Williams' Medium E
For certain mycological assays. with L-glutamine and 165 mM MOPS, without
sodium bicarbonate without L-glutamine
04-525F 500 ml BE12-761F 500 ml
Williams' Medium E
without L-glutamine or phenol red
BE02-019F 500 ml
259
Notes
260
Specialty Media
Specialty Media
Serum-free media and reagents have a wide range of Serum-free media formulations must satisfy a number
applications, including production of monoclonal antibodies, of nutritional and physical requirements of cells that
viral antigens, and recombinant proteins using a variety of are normally addressed by the presence of serum.
mammalian and invertebrate cell lines. There are numerous Serum proteins such as albumin, fibronectin, and fetuin
advantages associated with the use of serum-free media serve a variety of functions that include adsorbing toxic
formulations: compounds, providing protection against shear forces
■ Increased definition in bioreactors, creating a matrix for cellular attachment
to surfaces, and acting as a carrier for lipids and other
■ Increased lot-to-lot consistency growth factors.
■ Simplified purification and downstream processing
For information about adaptation of cell cultures to serum-
■ Better control over the physiological condition of cultures
free media, please refer to the Technical Information
■ Ability to optimize formulations for specific cell types section, page 298.
Specialty Media
Introduction 261 ProVero™ 1 Serum-free Medium 270
UltraCULTURE™ Serum-free Medium 262 ProPer™ 1 Chemically Defined, Serum-free Medium 271
PC-1™ Chemically Defined, Serum-free Medium 263 Human Tubal Fluid 272
UltraMEM™ Reduced Serum Media 264 Lymphochrome™ Medium 272
X-VIVO™ Chemically Defined, Serum-free Amniochrome® II Modified Medium 273
Hematopoietic Cell Media 265 Amniochrome® Plus Medium 273
UltraCHO™ Serum-free CHO Cell Media 266 Amniochrome® Pro Medium 273
ProCHO™ Protein-free CHO Media 267 HL-1™ Chemically Defined, Serum-free Media 274
PowerCHO® Chemically Defined, UltraDOMA™ Serum-free Hybridoma Medium 276
Serum-free CHO Media 268 ProDoma™ Serum-free Hydridoma Media 277
ProFreeze™ -CDM, NAO, Chemically Defined UltraDOMA-PF™ Protein-free Hydridoma Media 277
Freeze Medium (2X) 269 Insect-XPRESS™ Protein-free Insect Cell Medium 278
UltraMDCK™ Chemically Defined, ProNS0™ Chemically Defined, Protein-free Media 279
Serum-free Renal Cell Medium 269
Pro293™ Chemically Defined, Serum-free Media 270
261
UltraCULTURE™ Serum-free Medium
General purpose serum-free medium
UltraCULTURE™ Serum-free Medium is a complete, all- Ordering Information

purpose, serum-free medium designed for the cultivation
Cat. No. Size Price
of a wide variety of adherent and non-adherent
mammalian cell types. UltraCULTURE™ Medium can UltraCULTURE™ Serum-free Medium
be used to support fusion of cells during hybridoma without L-glutamine
formation, growth of monocyte, macrophage, epithelial, and 12-725F 500 ml
fibroblastic cells lines, and generation of virus particles
for vaccine production. The medium is supplemented with Partial list of cell cultures cultivated with UltraCULTURE™
Serum-free Medium
recombinant human insulin, bovine transferrin, and a Medium

UatraCULTURE™
purified mixture of bovine serum proteins, including

Cell Line Source Cell Type
albumin. The total protein concentration of UltraCULTURE™
HEL, N-10 Human Fetal lung diploid fibroblast
Medium is approximately 3 mg/ml. UltraCULTURE™ Medium
can be supplemented with Cryoprotective Medium HeLa Human Uterine cancer
(Cat. No. 12-132A) to cryopreserve cells in a serum-free HuL-1,2 Human Liver (normal)
environment. UltraCULTURE™ Medium does not contain HuK-1 Human Kidney (normal)
L-glutamine; add 5 ml of 200 mM L-glutamine solution HuS-1AT Human Skin
(Cat. Nos. 17-605 or 17-905) prior to use. HEC Human Embryonic cancer
HL-60 Human Acute promyelocytic leukemia
The formulation for UltraCULTURE™ Medium has been Raji Human Burkitt’s lymphoma
submitted to the FDA as a Master File. Permission to cross- EB-3 Human Burkitt’s lymphoma
reference the Master File may be obtained by contacting K-562 Human Chronic myelocytic leukemia
the Regulatory Affairs Department. HNK Human Neonatal kidney (primary)
HTC29 Human Colon cancer
■ Applications
TT Human Medullary thyroid tumor
– Cultivation of adherent and non-adherent mammalian MB231 Human Breast carcinoma
cells U138 Human Glioma
– Generation of viral particles for vaccine production FM3A Mouse Breast cancer
NS-1 Mouse Myeloma
L Mouse Subcutaneous
– Turbidity may develop in UltraCULTURE™ Medium; P388D1 Mouse Macrophage-like
experiments have determined that the turbidity will
P815 Mouse Mast cell tumor
not alter the performance of the product
T3 Mouse Pituitary
■ Storage Conditions B82 Mouse L cells - connective tissue
2°C – 8°C RPL-1 Rat Peritoneum
RSP-2 Rat Spleen
RLG-1 Rat Lung
Lym-1 Rat Lymph node
RCR-1 Rat Brain
235-1, MMQ Rat Pituitary
GC, GH3 Rat Pituitary
CA77 Rat Medulary thyroid tumor
Rat-1 Rat Fibroblast
JTC-12 Monkey Kidney
COS1, COS7 African green SV40 transformed kidney
monkey
Related Products
L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined, Freeze Medium (2X) 269
262
PC-1™ Chemically Defined, Serum-free Medium
General purpose serum-free medium
PC-1™ Chemically Defined, Serum-free Medium is a Ordering Information

low-protein, serum-free medium intended for the culture of
Cat. No. Size Price
primary cells and anchorage-dependent cell lines. PC-1™
Medium contains a complete HEPES buffering system PC-1™ Chemically Defined, Serum-free Medium
Complete medium system, including frozen supplement, without
with known amounts of insulin, transferrin, fatty acids, L-glutamine
and proprietary proteins assembled under strict quality
control procedures. PC-1™ Medium is intended for a variety 77232 2 × 500 ml
of research and industrial applications and is formulated
Serum-free Medium
using defined components for optimal cell growth, while
maintaining the lowest possible protein content.
■ Applications
PC-1™
– Cultivation of primary and anchorage-dependent cells
2°C – 8°C
Partial list of cell cultures cultivated with PC-1™ Chemically

Defined, Serum-free Medium Other cell types/sources include:
Cell Line Source Cell Type Source Cell Type

HeLa Human Epithelial carcinoma, cervix Human: Neuroblastoma, foreskin fibroblast, bladder carcinoma,
renal papillary collecting tubule (primary), colon
HTB-72 Human Malignant melanoma, epithelium (primary), colon carcinoma (primary)
epithelial-like
Rat: Dermal fibroblast (primary), mammary carcinoma
HTB-4 Human Bladder tumor (primary), neonatal normal cardiac muscle (primary),
WISH Human Amnion, epithelial-like thyroid epithelium (primary), astrocytes (primary)
WI-38 Human Lung, diploid Baboon: (Paprocynocephalus) spinal ganglia
HEP2 Human Transformed larynx, epidermoid Swine: Testes cell
carcinoma Bovine: Kidney
MRC-5 Human, male Embryonal lung, fibroblast-like
BHK-21 Syrian hamster Kidney, fibroblast-like
CHO-K1 Chinese hamster Ovary, epithelial-like
NRK Rat Normal kidney, epithelial/
fibroblast-like
C6 Rat Glioma, primary
T9 Rat Glioma
ARL6T Rat Normal liver
3T3 Mouse Embryonic, fibroblast-like
STO Mouse Transformed fibroblast
VERO African green monkey Fibroblast
MDCK Dog Madin Darby canine kidney,
epithelial-like
SIRC Rabbit Cornea
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L-glutamine 283
ProFreeze™-CDM, NAO, Chemically Defined, Freeze Medium (2X) 269
263
UltraMEM™ Reduced Serum Media
General purpose serum-free media
UltraMEM™ Reduced Serum Media are chemically- Ordering Information

defined media designed to support growth and
Cat. No. Size Price
maintenance of several anchorage-dependent
cell types under reduced serum concentrations UltraMEM™ Reduced Serum Medium
protein-free basal medium without L-glutamine
(see table). When supplemented with 2 – 4%
serum, UltraMEM™ Media growth performance is 12-745F 500 ml
comparable and, in some instances, exceeds that of
standard media supplemented with 10% fetal bovine UltraMEM™ Reduced Serum Medium
low protein medium containing ITES and L-glutamine
serum. Weaning is not necessary for most applications.
Serum-free Media
12-743F 500 ml
Further reduction in serum concentration (<2%) can be
UltraMEM™
achieved over several passages. In addition, confluent

cultures can be maintained with minimal amounts of
serum (≤1%) or no serum at all. Growth performance in Partial list of cell cultures cultivated with UltraMEM™
UltraMEM™ Media can be further increased by the addition Reduced Serum Medium
of insulin, transferrin, selenium, and ethanolamine (ITS
Cell Type Recommended Recommended %
or ITES) to the basal medium. UltraMEM™ Reduced Serum UltraMEM™1 Serum 2
Media are offered as a protein-free “basal” medium
Diploid
without L-glutamine, and as a “complete” low protein
CEF, chicken fibroblast with ITS/ITES 2% FBS
medium supplemented with ITES (Cat. No. 17-839Z).
WI-38, human fibroblast with or without ITES 3% FBS
Recombinant human insulin and transferrin are the only
RHMK, monkey kidney with or without ITS/ 2 – 4% FBS
protein components of the “complete” formulation and ITES
are present at a total concentration of 20 µg/ml. MRC-5, human fibroblast with ITS/ITES 3% FBS
■ Applications RK, rabbit kidney with ITS/ITES 3% FBS
BGMK, monkey kidney with ITS/ITES 2 – 3% FBS
– Growth and maintenance of anchorage-dependent
HEL, human fibroblast with ITS/ITES 2 – 3% FBS
cell types
CMK, monkey kidney with ITS/ITES 3 – 4% FBS
Heteroploid
2°C – 8°C
RD, human sarcoma without ITS/ITES 3 – 4% FBS
VERO, monkey kidney with or without ITS/ 3% FBS
ITES
CRFK, cat kidney with ITS/ITES 2 – 3% FBS
L929, mouse fibroblast with or without ITS/ 4% FBS
ITES
MDCK, dog kidney with ITS/ITES 2 – 3% FBS
McCOY, mouse fibroblast with ITS/ITES 4% FBS
3T3, mouse fibroblast with ITS/ITES 3 – 4% FBS
CHO-K1, hamster ovary with ITS/ITES 3% FBS
PK-15, pig kidney with ITS/ITES 3 – 4% FBS
MK2, monkey kidney with or without ITS/ 3 – 4% FBS
ITES
ITS/ITES should not be refrozen. It can be kept refrigerated (2°C – 8°C) for
up to a month.
1. ITS/ITES means either one supplement or the other.
2. In the following cell lines Fetal Bovine Serum can be replaced with Serum
Supreme (Cat. No. 14-492F): CRFK, MDCK, 3T3, CHO-K1, PK-15, BGMK
and MK2.
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ITES 283
ITS 283
264
X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Media
Serum-free media for hematopoietic cells
X-VIVO™ Chemically Defined, Serum-free Hematopoietic Ordering Information

Cell Media provide nutritionally complete and balanced
Cat. No. Size Price
environments for a variety of cells including lymphokine
activated killer (LAK) cells, peripheral blood lymphocytes X-VIVO™ 10 Chemically Defined, Serum-free
(PBL), and tumor infiltrating lymphocytes (TIL). These Hematopoietic Cell Medium
with L-glutamine, gentamicin, and phenol red
media do not contain any exogenous growth factors,
artificial stimulators of cellular proliferation, or undefined 04-380Q 1L
supplements. They are devoid of any protein kinase
C stimulators and are suitable for the investigation of X-VIVO™ 10 Chemically Defined, Serum-free
second messenger systems in the activation of human Hematopoietic Cell Medium
X-VIVO™ Media
with L-glutamine, without gentamicin or phenol red
and murine lymphocytes. The formulations are complete
and contain pharmaceutical grade human albumin, 04-743Q 1L
recombinant human insulin, and pasteurized human
transferrin. X-VIVO™ 15 Chemically Defined, Serum-free
Hematopoietic Cell Medium
with L-glutamine, gentamicin, and phenol red
All X-VIVO™ Media products are manufactured under current
GMPs and are listed with the FDA in a product Master File. BE04-418F 500 ml
Permission to cross-reference the Master File may be 04-418Q 1L
obtained by contacting the Regulatory Affairs Department.

X-VIVO™ 15 Chemically Defined, Serum-free
X-VIVO™ 10 Chemically Defined, Serum-free with L-glutamine, without gentamicin or phenol red
Hematopoietic Cell Media
04-744Q 1L
The X-VIVO™ 10 Media formulations are designed to
support the generation of LAK cells in a serum-free
X-VIVO™ 20 Chemically Defined, Serum-free
environment. The original protocols involved the incubation
of patient or normal donor peripheral blood lymphocytes with L-glutamine, gentamicin, and phenol red
(PBL) at 1.0 – 3.0 × 106 cells/ml for a period of 3 days in
04-448Q 1L
the presence of 1,000 Cetus units of rIL-2/ml. Optimal LAK
cell generation is achieved when peripheral blood
lymphocytes are incubated for 3 – 10 days at a density
of 1.0 – 6.0 × 106 cells/ml in the presence of 100 – 1,000 X-VIVO™ 20 Chemically Defined, Serum-free
Cetus units of rIL-2. X-VIVO™ 10 Media is available as a Hematopoietic Cell Medium
1X liquid in two convenient formulations. X-VIVO™ 20 Medium is optimized to support the generation
of lymphokine activated killer (LAK) cells from monocyte-
X-VIVO™ 15 Chemically Defined, Serum-free depleted peripheral blood lymphocytes (PBL) at high
Hematopoietic Cell Media density. Initial cell densities between 2.0 – 3.0 × 107
X-VIVO™ 15 Media are similar in composition to X-VIVO™ 10 cells/ml can be used to successfully generate LAK cells.
Media and have been optimized for the proliferation of X-VIVO™ 20 Medium may also be used as a growth medium
tumor infiltrating lymphocytes (TIL) under serum-free for PBL and tumor infiltrating lymphocytes (TIL).
conditions. X-VIVO™ 15 Media support the proliferation of ■ Applications
purified CD3 + cells isolated from peripheral blood and – Proliferation of peripheral blood lymphocytes
human tumors. X-VIVO™ 15 Media can also be used to
support the growth of human monocytes, macrophage – Proliferation of tumor infiltrating lymphocytes
cells and cell lines, PBL, granulocytes, and natural killer – Cryopreservation and transplantation of organs
(NK) cells. In addition, X-VIVO™ 15 Media provide a serum- – Cultivation of human monocytes and macrophages
free environment for the expansion of HUT-78 and related
human lymphocytic cell lines. – Cultivation of stem cells
– Cultivation of dendritic cells
2°C – 8°C
265
UltraCHO™ Serum-free CHO Cell Media
CHO expression media
UltraCHO™ Serum-free CHO Cell Media are optimized to Ordering Information

support the growth of transfected and non-transfected
Cat. No. Size Price
Chinese Hamster Ovary (CHO) cells and expression of
recombinant proteins. UltraCHO™ Media are composed UltraCHO™ Serum-free CHO Cell Medium
of a modified DMEM:F-12 base. The formulation is 1X liquid with L-glutamine
supplemented with insulin, transferrin, and proprietary 12-724Q 1L

purified proteins. UltraCHO™ Media are available as a
complete 1X liquid or as a powder with a frozen supplement. UltraCHO™ Serum-free CHO Cell Medium, Powder
UltraCHO™ Media contains less than 300 µg/ml protein. 15-724D 1 × 10 L
UltraCHO™ Media
15-724F 1 × 50 L
UltraCHO™ Media are manufactured under FDA current
GMPs and the formulation has been submitted to the FDA as UltraCHO™ Supplement
non-sterile, for use with UltraCHO™ Powdered medium
a Master File. Permission to cross-reference the file may be
obtained from the Regulatory Affairs Department. 17-811A 100 ml
■ Applications
– Growth of CHO cells
– Recombinant protein production
■ Storage Conditions ■ Partial list of cell types cultivated with UltraCHO™
Liquid medium: 2°C – 8°C Medium
Powdered medium: 2°C – 8°C – Transfected and non-transfected CHO cell lines
Supplement: -10°C – -20°C – HeLa cells (suspension or attached)

– Human leukemia cell lines
266
ProCHO™ Protein-free CHO Media
Non-animal origin CHO expression media
ProCHO™ Protein-free CHO Media were developed Ordering Information

specifically to facilitate the production and downstream
Cat. No. Size Price
processing of recombinant proteins expressed in CHO
cells. These protein-free formulations support high-density ProCHO™ 4 Protein-free CHO Medium
cultures without the need for animal derived components. with 0.1% Pluronic® F-68 and phenol red, without hypoxanthine, thymidine,
or L-glutamine
Very low levels of recombinant insulin facilitate both
downstream purification and regulatory compliance. The 12-029Q 1L
following media systems are available:

ProCHO™ 4 Protein-free CHO Medium
Protein-free Media
— ProCHO™ 4 Medium – For concurrent transition of with 0.1% Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or
L-glutamine
adherent CHO cells to serum-free and suspension
ProCHO™
culture; supports faster doubling times 04-919Q 1L
— ProCHO™ 5 Medium – For CHO cells already growing in ProCHO™ 4 Protein-free CHO Medium, Powder
suspension; supports increased protein production includes base powder and required supplements
with 0.1% Pluronic® F-68, without phenol red, hypoxanthine, thymidine, or
— ProCHO™ AT Medium – For adherent culture of CHO L-glutamine
cells BE15-029D 1 × 10 L
BE15-029F 1 × 50 L
ProMEDIA SELECT® CHO Media Optimization Kit
Kit contains 16 distinct formulas that contain no materials ProCHO™ 5 Protein-free CHO Medium
of animal origin. It can be used to develop the most L-glutamine
optimized formulas for protein expression in CHO cells.
Media formulations are scalable to production volumes. 12-766Q 1L
■ Applications ProCHO™ 5 Protein-free CHO Medium, Powder

– Recombinant protein expression in CHO cells L-glutamine
■ Storage Conditions BE15-766D 1 × 10 L

2°C – 8°C BE15-766F 1 × 50 L
ProCHO™ AT Serum-free CHO Medium

with L-glutamine, without hypoxanthine or thymidine; for adherent CHO
cells
BE02-016Q 1L
ProMEDIA Select® CHO Media Optimization Kit

without L-glutamine, hypoxanthine, or thymidine
BE18-002Q Kit, 16 x 1 L
Related Products
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) 269
ProHT™ Supplement (100X) 283
267
PowerCHO® Chemically Defined, Serum-free CHO Media
Non-animal origin CHO expression media
PowerCHO® 1, 2, and 3 Chemically Defined, Serum-free Ordering Information

CHO Media are the next generation in CHO media, optimized
Cat. No. Size Price
for both cell growth and protein production. PowerCHO®
Media are hydrolysate-free, serum-free, and non-animal PowerCHO® 1 Chemically Defined, Serum-free CHO
origin media for supporting high-density CHO cells in Medium
with HEPES and Pluronic® F-68, without phenol red, hypoxanthine,
suspension. For therapeutic bioprocessing applications, thymidine, or L-glutamine
these protein-free formulations also facilitate both
downstream purification and regulatory compliance. 12-770Q 1L
— Maximum culture performance through balanced

Serum-free Media
PowerCHO® 2 Chemically Defined, Serum-free CHO

formulation Medium
PowerCHO®

— Maintain high viability (>90%) at high cell densities thymidine, or L-glutamine
— Confidence in performance lot-to-lot with chemically 12-771Q 1L
defined, serum-free media
— Easily scaleable to support high-density, large scale PowerCHO® 2 Chemically Defined, Serum-free CHO
production volumes Medium, Powder
PowerCHO® -GS is designed for use with Lonza's Proprietary thymidine, or L-glutamine
GS Gene Expression System™. Please visit www.lonza. BE15-771D 10 L
com/geneexpressions for more information on the GS BE15-771J 100 L
System™.
PowerCHO® 3 Chemically Defined, Serum-free CHO
■ Applications Medium
– Recombinant protein expression in CHO cells thymidine, or L-glutamine
■ Storage Conditions 12-772Q 1L
2°C – 8°C PowerCHO®-GS Chemically Defined, Serum-free CHO

Medium
with HEPES and Pluronic® F-68, without insulin, phenol red or
L-glutamine
00189269 1L
PowerCHO® Media and ProCHO™ Media IgG production

2500
lgG (ng/ml)
2000
1500
1000
500
0 ProCHOTM 4 PowerCHO® 1 PowerCHO® 2 PowerCHO® 3 Commercial Commercial Commercial Commercial

Supplier A Supplier B Supplier C Supplier D
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L-glutamine 283
268
ProFreeze™- CDM, NAO, Chemically Defined Freeze Medium (2X)
Non-animal origin freezing medium
ProFreeze™- CDM, NAO, Chemically Defined Freeze Medium Ordering Information

(2X) is universally suitable for cryopreserving many
Cat. No. Size Price
cell types in the absence of fetal bovine serum (FBS).
However, it is used to greatest advantage with cells ProFreeze™- CDM, NAO, Chemically Defined Freeze
cultured in a serum-free and animal component-free Medium (2X)
environment. This protein-free freezing medium contains non-animal origin (NAO), chemically defined
Please see complete instructions at:
no animal derived components, insulin, or hydrolysate, www.lonza.com/profreeze
and maintains high cell viability upon recovery from 12-769E 100 ml
NAO Freezing Medium

frozen storage.
Renal Media
ProFreeze™ Medium requires the addition of 15% reagent
or spectrophotometric grade dimethylsulfoxide (DMSO) at
time of use. One bottle will make 117.6 ml of complete 2X
concentrated freezing medium after the addition of 17.6
ml DMSO. For best results, keep on ice during use.
2°C – 8°C
UltraMDCK™ Chemically Defined, Serum-free Renal Cell Medium

Renal cell expression medium
UltraMDCK™ Chemically Defined, Serum-free Renal Cell Ordering Information

Medium is a defined, serum-free medium designed
Cat. No. Size Price
to support the growth of MADIN-DARBY Canine Kidney
(MDCK) cells at low and high plating densities. UltraMDCK™ UltraMDCK™ Chemically Defined, Serum-free Renal Cell
Medium contains low levels of recombinant human Medium
with L-glutamine
insulin and bovine transferrin, yielding a very low protein
formulation. MDCK cells grown in UltraMDCK™ Medium are 12-749Q 1L
smaller and more densely packed than cells grown in the
presence of serum, and cultures can stay confluent for at
least two weeks without medium change.
■ Applications
– Growth of MDCK cells
2°C – 8°C
269
Pro293™ Chemically Defined, Serum-free Media
Non-animal origin renal cell expression media
Pro293™ Chemically Defined, Serum-free Media Ordering Information

were optimized to support high-density growth and
Cat. No. Size Price
recombinant protein production in 293 neonatal kidney
cells. They are chemically defined to ease regulatory Pro293™s Chemically Defined, Serum-free Medium
without L-glutamine or phenol red, with 0.1% Pluronic® F-68
compliance and downstream protein purification. They
contain very low levels of recombinant human insulin, 12-765Q 1L
and are free of animal-origin components.
Pro293™a Chemically Defined, Serum-free Medium
— Pro293™s Medium – for 293 cells growing in
Pro293™ and ProVero™
without L-glutamine or phenol red, with 0.1% Pluronic® F-68

suspension culture
12-764Q 1L
Renal Media
— Pro293™ a Medium– for 293 cells growing in adherent

culture
■ Applications
– Recombinant protein production in 293 cells
2°C – 8°C
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L-glutamine 283
ProVero™ 1 Serum-free Medium

Non-animal origin renal cell expression medium
ProVero™ 1 Serum-free Medium is a protein-free medium Ordering Information

designed to support the growth of MDCK and vero
Cat. No. Size Price
cells. ProVero™ 1 Medium includes HEPES and sodium
bicarbonate buffer. The absence of proteins and only very ProVero™ 1 Serum-free Medium
low levels of human recombinant insulin facilitate both with L-glutamine
downstream processing and regulatory compliance. BE02-030Q 1L
Some vero cell strains require additional supplementation

with 5.0 µg/L rhEGF for optimal vero cell growth.
■ Applications
– Recombinant protein production
– Virus production
2°C – 8°C
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ProFreeze™- CDM, NAO, Chemically-Defined Freeze Medium (2X) 269
270
ProPer™ 1 Chemically Defined, Serum-free Medium
Non-animal origin medium for human embryonic retinoblast cells
ProPer™ 1 Chemically Defined, Serum-free Medium is an Ordering Information

animal origin component-free, chemically defined, serum-
Cat. No. Size Price
free medium for growth of human embryonic retinoblast
cells (PER.C6® and related cell lines) in suspension. The ProPer™ 1 Chemically Defined, Serum-free Medium
medium contains a low amount of human recombinant with 0.1% Pluronic® F-68, without phenol red, or L-glutamine
insulin. HEPES as well as sodium bicarbonate is present BE02-028Q 1L
in the formulation.
■ Applications
Serum-free Medium
– Recombinant protein and virus production
– Growth of human embryonic retinoblast cells (PER.C6®
ProPer™ 1
and related cell lines) in suspension
2°C – 8°C
PER.C6® cells growing in ProPer™ 1 Medium.
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L-glutamine 283
271
IVF Culture Media
Mouse embryo tested
Human Tubal Fluid
HTF with Gentamicin Ordering Information

Recommended for IVF, GIFT and ZIFT procedures.
Cat. No. Size Price
HTF with Gentamicin
2°C – 8°C
Specialty Media
BE02-036F 500 ml
IVF Culture
HTF HEPES with Gentamicin

BE02-037F 500 ml
Lymphochrome™ Medium
Karyotyping medium
Complete, ready-to-use, serum-free medium for the Ordering Information

cultivation of lymphocytes from peripheral blood.
Cat. No. Size Price
Serum-free medium provides a high lot-to-lot consistency
with high mitotic index and good chromosome pattern. For Lymphochrome™ Medium
with L-glutamine and phytohaemagglutinin
in vitro diagnostic use.
■ Storage Conditions BE02-015E 100 ml
BE02-015BOX 10 × 5 ml
-10°C – -20°C
BE02-015F 500 ml
CE marked according to IVD Directive 98/79/EC
272
Cytogenetics Media
Amniochrome® II Modified Medium

Cytogenetics research
Amniochrome® II Modified Medium has been developed Ordering Information

for the culture of human amniotic fluid cells obtained
Cat. No. Size Price
from amniocentesis, a procedure extensively used for
clinical prenatal diagnosis. The Amniochrome® II Modified Amniochrome® II Modified Medium
Medium is performance tested by a cytogenetic reference
Specialty Media
BE12-756EZM 100 ml
laboratory for the cultivation of amniotic fluid cells for
Cytogenetics
BE12-756FCM 500 ml
cytogenetic analysis.
Complete medium for the primary culture of amniotic fluid

and chorionic villi cells used in cytogenetic applications.
The system includes an enriched basal medium and
growth supplement. For diagnostic use.
Basal medium: 2°C – 8°C
Supplement: -10°C – -20°C
Amniochrome® Plus Medium

Cytogenetics research
Complete, ready-to-use medium for the primary culture of Ordering Information

amniotic fluid and chorionic villi cells used in cytogenetic
Cat. No. Size Price
applications. Quick attachment of the cells and high
growth speed of cells are the main advantages of this new Amniochrome® Plus Medium
media formulation. For in vitro diagnostic use. BE02-026E 100 ml
■ Storage Conditions BE02-026F 500 ml
-10°C – -20°C CE marked according to IVD Directive 98/79/EC
Amniochrome® Pro Medium

Cytogenetics medium
Complete, ready-to-use medium for the primary culture of Ordering Information

amniotic fluid and chorionic villi cells used in cytogenetic
Cat. No. Size Price
applications. Contains the necessary growth factors,
L-glutamine, phenol red, sodium bicarbonate, and FBS. Amniochrome® Pro Medium
The complete formulation reduces handling steps and the BE02-035E 100 ml
possibility of contamination. For diagnostic use.
BE02-035F 500 ml
-10°C – -20°C
273
HL-1™ Chemically Defined, Serum-free Media
Chemically defined hybridoma medium
HL-1™ Chemically Defined, Serum-free Media is a culture Ordering Information

medium containing less than 30 µg protein per ml.
Cat. No. Size Price
Components of HL-1™ Medium include HEPES buffer,
insulin, transferrin, sodium selenite, ethanolamine, a HL-1™ Chemically Defined, Serum-free Medium
1X liquid, without L-glutamine, HL-1™ Supplement (77227) is not required.
variety of saturated and unsaturated fatty acids and
proprietary stabilizing proteins. HL-1™ Medium contains 77201 2 × 500 ml
no bovine serum albumin or other undefined protein
mixtures. HL-1™ Medium supports the serum-free growth HL-1™ Chemically Defined, Serum-free Medium, Powder
without L-glutamine, HL-1™ Supplement (77227) is not required.
of various hybridomas, including those derived from
Hybridoma Media
HL-1™ Serum-free
P3X63Ag8.653 and Sp2/0-Ag14 myelomas, as well as 77204 50 L

other differentiated cells of lymphoid origin.
HL-1™ Chemically Defined, Serum-free Medium
■ Applications
Supplement (100X)
– Serum-free growth of hybridomas and differentiated
77227 10 ml
cells of lymphoid origin
2°C – 8°C
HL-1™ Chemically Defined, Serum-free Medium

Supplement (100X)
HL-1™ Chemically Defined, Serum-free Medium Supplement
(100X) is a chemically defined medium additive that
can be used to replace serum or significantly reduce its
concentration in a variety of basal media. It contains less
than 30 µg protein/ml when diluted 1:100 in medium
and it does not contain bovine serum albumin or other
undefined protein ingredients.
15°C – 30°C
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274
HL-1™ Chemically Defined, Serum-free Media
Continued
Partial list of cell cultures cultivated with HL-1™ Chemically Defined, Serum-free Medium
Transformed and established cell lines Hybridomas
Cell Line Source Cell Type Hybridoma Source Fusion Partner

U937 Human Macrophage 8A1 Human CLLC
RaJi Human B lymphoblastic undesignated Human WI-L2-729-HF2
MCF-7 (NIH) Human Breast carcinoma undesignated Human LICR-LON-HMY2
MCF-7 (MCF) Human Breast carcinoma HB44 Mouse Sp2/0-Ag14
Hybridoma Media
HL-1™ Serum-free
NIH ZR-75 Human Breast carcinoma HB45 Mouse Sp2/0-Ag14
COLO 302 HSR Human Colon carcinoma HB56 Mouse NS-1
J82 Human Bladder carcinoma HB59 Mouse NS-1
SW 1738 Human Bladder carcinoma HB60 Mouse P3X63Ag 8.653
SW780 Human Bladder carcinoma 53-7.313 Mouse NS-1
CCL 119 Human Lymphoid MI/9.3.4HL-2 Mouse NS-1
CCL 213 Human Burkitt lymphoma MI/70.15.1 Mouse NS-1
C91/PL Human T lymphoma ARB Mouse Hybridoma
Undesignated Human Astrocytoma P3U Mouse P3X63Ag 8.653
Undesignated Human Hepatoma BCS12 Mouse P3X63Ag 8.653
MOLT-3 Human Acute lymphoblastic leukemia BCS 2002 Mouse P3X63Ag 8.653
MOLT-4 Human Acute lymphoblastic leukemia undesignated Mouse NS-1
NAMALWA Human Burkitt lymphoma undesignated Mouse P3X63Ag 8.653
THP-1 Human Monocytic leukemia TIB 175 Rat/mouse S194
BB88 Mouse Erythroid (leukemia) TIB 104 Rat/mouse NS-1
P815 Mouse Macrophage TIB 105 Rat/mouse NS-1
P388D1 Mouse Macrophage TIB 109 Rat/mouse NS-1
WeHi3 Mouse Monocyte TIB 128 Rat/mouse NS-1
JLS-V5 Mouse Spleen cell TIB 166 Rat/mouse NS-1
70Z-3 Mouse Pre-B lymphoma TIB 168 Rat/mouse NS-1
70Z/3.12 Mouse B lymphoma RS Rat/mouse P3X63Ag 8.653
S49 and variants Mouse T lymphoma
RAW309F1.1 Mouse T lymphoma
WeHi7 Mouse T lymphoma
Primary cells
I-10 Mouse Leydig-tumor
EL4 Mouse T lymphoma Cell Type
RL1 Mouse T lymphoma Human peripheral blood mononuclear cells
BW5147.3 Mouse T lymphoma Mink lymphocytes
LBRM-33 Mouse T lymphoma Human fetal adrenal
Friend leukemia Mouse Leukemia Human blood monocytes
C57BL6 Mouse Embryo (C57 X DBA) Human peripheral blood T lymphocytes
L5178Y Mouse Lymphoma (DBA/2)
VERO African green monkey Fibroblast
MDCK Dog Madin Darby canine kidney
CHO K1 Hamster Chinese hamster ovary
(epithelial-like)
GCL2 Hamster/mouse B lymphoma X Normal B
275
UltraDOMA™ Serum-free Hybridoma Medium
Hybridoma medium
UltraDOMA™ Serum-free Hybridoma Medium is a Ordering Information

formulation designed for the cultivation of murine, human,
Cat. No. Size Price
and chimeric hybridomas in batch culture and in hollow
fiber bioreactors. UltraDOMA™ Medium is supplemented UltraDOMA™ Serum-free Hydridoma Medium
with recombinant human insulin, bovine transferrin and without L-glutamine
bovine albumin. The total protein concentration is 30 µg/ 12-723B 500 ml (glass bottle)
ml. UltraDOMA™ Medium does not contain L-glutamine. BE12-723F 500 ml (plastic bottle)
UltraDOMA™ Serum-free
Cells that are adapted for growth in UltraDOMA™ Medium

Hybridoma Media
can be maintained in the medium indefinitely and can be Partial list of cell types cultivated with UltraDOMA™ Medium
cryopreserved in UltraDOMA™ Medium supplemented with
Cell Type
Cryoprotective Freezing Medium (Cat. No. 12-132A).
Murine hybridomas
■ Applications NS-1 derived myelomas
– Hybridoma cell growth SP-2 derived myelomas
– Monoclonal antibody production Human hybridomas (with 0.5% FBS)

– UltraDOMA™ Medium contains no human-derived
proteins
2°C – 8°C
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L-glutamine 283
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276
ProDoma™ Serum-free Hybridoma Media
Non-animal origin hybridoma media
ProDoma™ Serum-free Hybridoma Media have been Ordering Information

developed for cultivation of murine, human, and chimeric
Cat. No. Size Price
hybridomas. ProDoma™ Media are protein-free with a low
amount of human recombinant insulin. All ProDoma™ ProDoma™ 1 Chemically Defined, Serum-free Hydridoma
Media include HEPES as well as sodium bicarbonate in the Medium
with 0.1% Pluronic® F-68, without phenol red, or L-glutamine
formulation.
BE02-029Q 1L
■ Applications
– Hybridoma cell growth ProDoma™ 3 Serum-free Hydridoma Medium
Hybridoma Media
with 0.1% Pluronic® F-68, without phenol red, or L-glutamine
– Monoclonal antibody production
BE02-032Q 1L
2°C – 8°C
UltraDOMA-PF™ Protein-free Hydridoma Media

Non-animal origin hybridoma media
UltraDOMA-PF™ Protein-free Hydridoma Media are Ordering Information

formulations designed for use with hybridoma cell lines
Cat. No. Size Price
of murine, human, and chimeric origin. UltraDOMA-PF™
Media are completely defined media and do not contain UltraDOMA-PF™ Protein-free Hydridoma Medium
peptides or tissue extracts. The use of UltraDOMA-PF™ 1X liquid; with L-glutamine
Media significantly simplifies downstream processing 12-727F 500 ml

since all proteins present in a given cell culture supernatant
are produced by the cells. UltraDOMA-PF™ Media are UltraDOMA-PF™ Protein-free Hydridoma Medium,
designed for lab scale or industrial scale use. It is available as Powdered
a 1X liquid or as a powder. L-glutamine and HEPES buffer 15-727D 1 × 10 L
are included in the formulation.
■ Applications Partial list of cell types cultivated with UltraDOMA-PF™
– Hybridoma and myeloma growth Medium
– Monoclonal antibody production Cell Type
■ Storage Conditions Murine hybridomas

NS-1 derived myelomas
2°C – 8°C
SP-2 derived myelomas
Human hybridomas (with 0.5% FBS)
Rat hybridomas
Some transfected Chinese Hamster Ovary (CHO) cell lines
Human lymphoid origin cells
Murine lymphoid origin cells
277
Insect-XPRESS™ Protein-free Insect Cell Medium
Insect cell expression medium
Insect-XPRESS™ Protein-free Insect Cell Medium is a Ordering Information

formulation designed to support the growth of insect cell
lines derived from Spodoptera frugiperda (Sf9 and Sf21). Cat. No. Size Price
Cell densities in excess of 8.3 × 106 cells/ml can be achieved Insect-XPRESS™ Protein-free Insect Cell Medium
with suspension cultures of Sf9 cells using Insect-XPRESS™ with L-glutamine
Medium and an excess of oxygen. This formulation can 12-730F 500 ml
also be used for stationary monolayer cultures and 12-730Q 1L
shake-flask cultures. Insect-XPRESS™ Medium contains
Protein-free Medium
L-glutamine and supports superior production of recom-

Insect-XPRESS™
binant proteins when using the Baculovirus Expression

Vector System (BEVS). For cryopreser vation of insect
cells, Insect-XPRESS™ Medium can be mixed 50:50 with
Cryoprotective Freeze Medium (Cat. No. 12-132A).
■ Applications
– Baculovirus propagation
– Recombinant protein expression
2°C – 8°C
Related Products
278
ProNS0™ Chemically Defined, Protein-free Media
Non-animal origin NS0 expression media
The NS0 cell line is widely used for recombinant mammalian Ordering Information
protein expression. Some of the reasons to select this mouse
Cat. No. Size Price
myeloma platform are:
— Forms stably producing hybrid cells with high levels of ProNS0™ 1 Chemically Defined, Protein-free Medium
with HEPES and Pluronic®, without L-glutamine, phenol red, or cholesterol
protein production
12-773Q 1L
— Lacks ability to secrete endogenous antibody or
antibody fragments
ProNS0™ 2 Chemically Defined, Protein-free Medium
— Resists aggregation clumping in suspension culture with HEPES and Pluronic®, without L-glutamine, phenol red, or cholesterol
Protein-free Media
— Lacks adverse protease activity on recombinant 12-774Q 1L
ProNS0 ™
protein product (in many cases)
ProNS0™ Lipid Chemically Defined Supplement
ProNS0™ 1 and 2 CD Media, together with ProNS0™ Lipid with cholesterol, suggested use is 5 ml/L.
CD Supplement, are designed to meet a growing demand
12-775J 5 ml
for optimized NS0 formulations.
— Two optimized formulas are available to cover the
broadest range of NS0 nutritional needs Protein Production – ProNS0™ Media
IgG (mg/ml)
0.2 Day 6 mg/ml
— Further maximize protein production by titration with
ProNS0™ Lipid CD Supplement 0.15
— Protein-free and chemically defined formulations

0.1
– Product purification is simplified
– Lot-to-lot consistency ensures dependable 0.05
performance
0 ProNS0™1 CD ProNS0™2 CD Competitor 1 Competitor 2 Competitor 3
— Non-animal origin components reduce regulatory
Figure 1. ProNS0™ Media outperform competitive media for recombinant
burdens IgG production in NS0 cells. Duplicate 125 ml shaker flasks were seeded
at a density of 200,000 NS0 cells per ml in a 30 ml volume. Shake rate was
The data in Figure 1 show that both ProNS0™ 1 and 2 100 rpm. Cells were cultured in their respective test media for one passage
CD Media yield superior protein production compared to prior to test initiation.
competitive media. Other experiments displayed the
same trends (data not shown).
■ Applications
– High density culture of NS0 cells
Basal media: 2°C – 8°C
Lipid supplement: -10° – -20°C
Related Products
L-glutamine 283
279
Notes
280
BioWhittaker® Cell Culture Reagents include a number of Chemicals used to prepare cell culture reagents are
different products, such as amino acids, antibiotics, and purchased according to the raw material qualifications
buffers, all of which are used routinely in research, and from approved suppliers. Each lot must meet established
manufacturing applications involving cell culture. component specifications before it is released by Quality
Assurance for use. We manufacture all liquid cell culture
Products are manufactured under the same cGMP conditions reagents using Water for Injection (WFI) quality water,

as our other cell culture products. which has been prepared by ultrafiltration, reverse osmosis,
deionization, and distillation. Liquid products are sterile
filtered through pharmaceutical-grade sterilizing filters.

Reagents 283
281
Balanced Salt Solutions
notE: All balanced salt solutions contain phenol red and Hanks’ BSS (1X)
naHCo3, unless otherwise indicated.
Without calcium or magnesium

With calcium and magnesium

Cat. No. Size Price
Without phenol red

Earle’s BSS
With phenol red

with phenol red
Cat. No. Size
BE10-502F 500 ml
10-508F 500 ml
■ ■
10-508Q 1L
Earle’s BSS
with 20 mM HEPES, 1.8 g/L sodium bicarbonate, and phenol red 10-543F 500 ml
■ ■
Storage Conditions: 15°C – 30°C 10-543Q 1L
10-527F 500 ml ■ ■
BE02-027F 500 ml
10-547F 500 ml ■ ■
04-315Q 1L ■ ■
08-003A 4 L (bag) ■ ■
Please see the Reagents Formulations section for exact compositions.

Balanced Salt Solutions
Cat. No. Size Price
Hanks’ BSS (1X)

with phenol red, calcium and magnesium
10-508F 500 ml
10-508Q 1L
Hanks’ BSS (1X)

with phenol red, without calcium or magnesium
10-543F 500 ml
10-543Q 1L
Hanks’ BSS (1X)

without phenol red
10-527F 500 ml
10-527F/12 12 × 500 ml
Hanks’ BSS (1X)

without phenol red, without calcium or magnesium
10-547F 500 ml
04-315Q 1L
08-003A 4L
the 4 L size is provided in a flexible package with one female luer connector,
one blood spike receptacle, and one male MPC connector with cap.
282
Reagents
ITS (500X) MEM Non-Essential Amino Acid Solution (10 mM, 100X)
Supplement of insulin, transferrin, and selenium 100X stock solution contains a 10 mM concentration of each non-essential
Storage Conditions: -10°C – -20°C amino acid
17-838Z 5 ml
13-114E 100 ml
ITES (500X)
Supplement of insulin, transferrin, ethanolamine, and selenium MEM Eagle Vitamin Mixture (100X)
Storage Conditions: -10°C – -20°C Storage Conditions: -20°C
17-839Z 5 ml 13-607C 50 ml
Lymphocyte Separation Medium, 1.077 ProHT™ Supplement (100X)

Density 1.077, for the isolation of human lymphocytes Hypoxanthine, thymidine supplement (100x) from non-animal origin;
Storage Conditions: 15°C – 30°C optimized for use with ProCHo™ Media (12-766Q, 12-029Q, 04-919Q,
BE02-016Q)
17-829E 100 ml Storage Conditions: -10°C – -20°C
17-829E/24 12 100 ml
BE17-855E 100 ml
L-glutamine (200 mM) Sodium Bicarbonate Solution, 7.5%

Supplied at 29.3 mg/ml in 0.85% naCl
Storage Conditions: -10°C – -20°C 7.5% aqueous solution
17-605C 50 ml
17-613E 100 ml
Reagents
17-605E 100 ml
17-605F 500 ml Sodium Bicarbonate (Powder)
L-glutamine (200 mM) 15-613I 500 g
Supplied at 29.3 mg/ml in 0.85% naCl and hybridoma screened
Sodium Pyruvate Solution (100 mM)
17-905C 50 ml 11.1 g/L
L-glutamine (Powder) 13-115E 100 ml

15-605G 25 g Trypan Blue 0.4% Solution

Prepared in 0.85% naCl
17-942E 100 ml
283
Reagents
Continued

Trypsin 1:250 (10X) 2.5% Versene® (EDTA) 0.02%

2.5% in Modified Hanks’ BSS without calcium or magnesium; manufactured 0.2 g/L Ethylenediaminetetraacetic acid (0.53 mM) in DPBS, without calcium
with irradiated porcine trypsin; tested for porcine parvovirus and mycoplasma or magnesium
Storage Conditions: -10°C – -20°C Storage Conditions: 15°C – 30°C
17-711E 100 ml
17-160E 100 ml
17-160F 500 ml
Water for Cell Culture
Water for Injection (WFI) quality water is prepared by ultrafiltration, reverse
Trypsin /EDTA (10X) osmosis, deionization, distillation, and sterile filtration
Contains 5 g/L trypsin 1:250 and 2 g/L Versene® (EDtA); manufactured with Storage Conditions: 15°C – 30°C
irradiated porcine trypsin; tested for porcine parvovirus and mycoplasma
Storage Conditions: -10°C – -20°C 17-724F 500 ml
BE02-007E 100 ml 17-724Q 1L
04-585P 20 L (carboy, screw-cap)
Trypsin-Versene® (EDTA) Mixture (1X) 08-199F 20 L (flexible packaging)
Contains 0.5 g/L trypsin 1:250 and 0.2 g/L Versene® (EDtA); manufactured
with irradiated porcine trypsin; tested for porcine parvovirus and mycoplasma 08-199D 100 L (flexible packaging)
Storage Conditions: -10°C – -20°C 08-199E 200 L (flexible packaging)
17-161E 100 ml BE02-038P20 20 L
17-161F 500 ml BE02-038P200 200 L
Please call for custom WFI products information.

UltraGlutamine I Supplement 200 mM (100X)
Reagents
200 mM Alanyl-L-glutamine in normal saline

BE17-605E/U1 100 ml
UltraGlutamine II Supplement 200 mM (100X)

200 mM L-Gycyl-L-glutamine in normal saline
BE04-684E 100 ml
284
Antibiotics and Antimycotics
Amphotericin B Penicillin-Streptomycin Mixture

contains 250 µg/ml amphotericin B contains 5,000 units potassium penicillin and 5,000 µg streptomycin
Storage Conditions: -10°C – -20°C sulfate per ml in 0.85% saline
17-836R 20 ml
17-603E 100 ml
17-836E 100 ml

contains 10,000 units potassium penicillin and 10,000 µg streptomycin
screw cap vial sulfate per ml in 0.85% saline
Storage Conditions: 15°C – 30°C Storage Conditions: -10°C – -20°C
17-518Z 1 × 10 ml 17-602E 100 ml
17-518L 10 × 10 ml 17-602F 500 ml

crimp top vial contains 20,000 units potassium penicillin and 20,000 µg streptomycin
Storage Conditions: 15°C – 30°C sulfate per ml in 0.85% saline
17-528Z 1 × 10 ml
09-757F 500 ml
Gentamicin Sulfate 10 mg/ml
screw cap vial
and Antimycotics
Penicillin-Streptomycin Mixture
Storage Conditions: 15°C – 30°C contains 25,000 units potassium penicillin and 25,000 µg streptomycin
Antibiotics
sulfate per ml in 0.85% saline
17-519Z 1 × 10 ml Storage Conditions: -10°C – -20°C
17-519L 10 × 10 ml
17-719R 25 × 4.5 ml
Gentamicin Sulfate
10 mg/ml Penicillin-Streptomycin-Amphotericin B Mixture
Storage Conditions: 15°C – 30°C contains 10,000 units potassium penicillin, 10,000 µg streptomycin sulfate
and 25 µg Amphotericin B per ml in 0.85% saline
BE02-012E 100 ml Storage Conditions: -10°C – -20°C
17-745H 20 ml
17-745E 100 ml
Penicillin-Streptomycin-L-glutamine Mixture
contains 25,000 units potassium penicillin, 25,000 µg streptomycin sulfate,
and 29.2 mg L-glutamine per ml
Penicillin-Streptomycin Mixtures
17-718R 25 × 4.5 ml
25 µg/ml Amphotericin B
20,000 µg streptomycin
20,000 units penicillin /

With L-glutamine
Cat. No. Size

17-603E 100 ml ■
17-602E 100 ml
■
17-602F 500 ml
09-757F 500 ml ■
17-719R 25 ✕ 4.5 ml ■
17-745H 20 ml
■ ■
17-745E 100 ml
17-718R 25 ✕ 4.5 ml ■ ■
Please see the Reagents Formulations section for exact compositions.

285
Buffers and Buffered Salines
ACK Lysing Buffer (1X) Dulbecco’s Phosphate Buffered Saline (1X)

used to lyse red blood cells in preparations containing white blood cells 0.0095 M (Po4) with calcium and magnesium
10-548E 100 ml 17-513F 500 ml

17-513Q 1L
Dextrose-Gelatin-Veronal (DGV)
Dulbecco’s Phosphate Buffered Saline (1X)
0.0095 M (Po4) without calcium or magnesium
10-539B 500 ml Storage Conditions: 15°C – 30°C
17-512F 500 ml
Dulbecco’s Phosphate Buffered Saline 17-512F/12 12 x 500 ml
17-512F/24 24 x 500 ml
17-512Q 1L
With calcium and magnesium
17-512Q/12 12 x 1 L
With sodium pyruvate
Dulbecco’s Phosphate Buffered Saline, Powder

Without phenol red
0.0095 M (Po4) without calcium or magnesium; this is a powdered version

With glucose
of 17-512
Powder
Buffered Salines
Cat. No. Size 15-456D

Buffers and
1 × 10 L
17-513F 500 ml
17-513Q 1L
■ ■ 15-456F 1 × 50 L
17-512F 500 ml BE15-512D 1 × 10 L
■ ■
17-512Q 1L
BE15-512F 1 × 50 L
15-456D 1 ✕ 10 L
15-456F 1 ✕ 50 L
BE15-512D 1 ✕ 10 L
■ ■ ■ Dulbecco’s Phosphate Buffered Saline (1X)
BE15-512F 1 ✕ 50 L 0.0095 M (Po4) with calcium, magnesium, 1 g/L glucose, and 36 mg/L
sodium pyruvate. Used in animal embryo transfer procedures.
04-479Q 1L ■ ■ ■ ■ Storage Conditions: 15°C – 30°C
17-515F (10X) 500 ml
■ ■ 04-479Q 1L
17-515Q (10X) 1L
Please see the Reagents Formulations section for exact compositions. Dulbecco’s Phosphate Buffered Saline (10X)
17-515F 500 ml
17-515Q 1L
HEPES Buffer (1 M)
contains 238.3 g/L in normal saline
17-737E 100 ml
17-737F 500 ml
286
Buffers and Buffered Salines
Continued
Ordering Information Buffered Saline
Cat. No. Size Price

PBS-ET
PBS used in animal embryo transfer procedures; bottle with septum cap
Without phenol red

BE02-018t 1L
With HEPES
PBS EDTA
used as buffer in procedures of generating human dendritic cells from Cat. No. Size
monocytes, pH 7.5 solution Phosphate Buffered Saline
17-516F 500 ml
■ ■
BE02-017F 500 ml 17-516Q 1L
04-409R (Carboy) 15 L ■ ■
Phosphate Buffered Saline (1X) 17-517Q (10X) 1L ■ ■

Storage Conditions: 15°C – 30°C UltraSaline A
12-747F 500 ml ■ ■
17-516F 500 ml
17-516F/12 12 x 500 ml Please see the Reagents Formulations section for exact compositions.
17-516F/24 24 x 500 ml
17-516Q 1L
Buffered Salines
17-516Q/12 12 x 1 L
Buffers and
Phosphate Buffered Saline (1X)
04-409R 15 L
Carboy with dispensing rigging, one male luer fitting and 5 feet of tubing.
Phosphate Buffered Saline (10X)

17-517Q 1L
TL HEPES Solution
04-616F 500 ml
UltraSaline A
HEPES buffered saline without phenol red; enhances trypsin action when
used to rinse monolayer before subculture
12-747F 500 ml
Veronal Buffer (5X)

12-624E 100 ml
287
Notes
288
BioWhittaker® Brand Sera and Media products from Lonza All sera, including bovine, equine and human, are aseptically
have been the leaders in quality for over 50 years. Our collected and must meet established component specifi-
strict adherence to current Good Manufacturing Practices cations before they are accepted and released by Quality
(cGMP) results in the highest quality serum products Assurance for use in further manufacturing. Daily
available. From raw material collection to final product collection records indicate the day, month and year the
release, in-process quality assurance tests are designed raw blood was collected and provide traceability to the
to ensure that the products we supply meet our own individual pool, which provides traceability back to the
high quality standards as well as our clients’ stringent USDA establishment number and abattoir or donor herd
requirements. from which the raw material originated.
All US-origin animal sera are collected from abattoirs Prior to filtration, the serum is dispensed into a batch
inpected by the USDA and located within the contiguous tank and mixed to assure bottle-to-bottle homogeneity.
48 states. USDA approved source fetal bovine serum (Cat. All fetal bovine serum is sterile filtered through three
No. 14-502) is USDA certified and approved for importation 0.1-micron filters.
into the United States.

Introduction 289
Equine Sera 294
Bovine Sera 295
Human Sera 295
Fetal Bovine Sera
289
Continued
Quality Assurance Sterility – Tests are performed on representative samples

using a modification of the membrane filtration method
Lonza produces a variety of serum products for use in
described in US Pharmacopeia (USP). A representative
cell culture media, production of biological and diagnostic
sample of serum is filtered. Filters are subsequently
products and other laboratory procedures that demand
immersed in fluid thioglycolate medium (FTM) and
the finest quality serum available. In order to maintain
trypticase soy broth (TSB). TSB cultures are incubated
consistent quality in serum products, strict quality
at 22.5°C ± 2.5°C. FTM cultures are incubated at 32.5°C ±
assurance of each production lot is maintained.
2.5°C. The cultures are incubated for 14 days, during which
they are periodically examined to ensure absence of
Raw Sera Quality Control microbial contamination.
All individual pools of raw serum are evaluated and must
meet established specifications before they are released Mycoplasma – Final products are tested for mycoplasma
for further manufacturing. Each pool must be free of by two separate methods: the large volume biological test
detectable mycoplasma and meet stringent specifications (Barile and Kern) and a DNA staining technique (Hoechst
for endotoxin, hemoglobin, pH, osmolality and protein. method).
Fetal bovine serum lots are also tested for adventitious
bovine viruses prior to acceptance. Only those pools Adventitious Viruses – All bovine serum lots receive full
meeting our standards of quality are accepted for use compliance 9CFR testing for adventitious viral agents
in further manufacturing. including bovine viral diarrhea (BVD), infectious bovine
rhinotracheites (IBR), parainfluenza virus type 3 (PI3),
Finished Product Quality Control bovine parvovirus, bovine adenovirus 3 and 5, rabies virus,
Samples of each lot of finished product are selected blue tongue, BRSV and reovirus. All donor horse serum lots
following 9CFR guidelines and tested in accordance with receive full compliance 9CFR testing for adventitious viral
written final product specifications. Final product quality agents including BVD, ERV/EHV-1, EVA, EIA, rabies virus,
control tests include the following: RED, IBR (CPE) and PI3 (hemadsorption).
Serum Donor
Assay FBS Calf Newborn Supreme Horse Human

Mycoplasma Negative Negative Negative Negative Negative Negative
Virus Full 9CFR Tested Full 9CFR Tested Full 9CFR Tested Full 9CFR Tested Full 9CFR Tested Tested and
Reported*
USP Sterility Negative Negative Negative Negative Negative Negative
Endotoxin ≤10 ≤25 Tested and ≤25 ≤25 ≤10
EU/ml Reported
Hemoglobin ≤20 ≤50 Tested and ≤50 ≤20 **
mg/dl Reported
pH 6.8 – 8.3 6.8 – 8.3 6.8 – 8.3 6.8 – 8.3 6.8 – 8.3 6.8 – 8.3
Osmolality 291 – 327 277 – 341 281 – 321 Tested and 270 – 305 271 – 307
Reported
Biochemical Tested and Tested and Tested and Tested and Tested and N/A
Profile Reported Reported Reported Reported Reported
Growth ≥75 % of control ≥75 % of control ≥75 % of control ≥75 % of control ≥75 % of control N/A
Promotion
Filtration 3 × 0.1 micron 3 × 0.2 micron 3 × 0.2 micron 3 × 0.2 micron 3 × 0.2 micron 2 × 0.2 micron
filtered filtered filtered filtered filtered filtered
*Virus testing completed on raw serum, not finished product.

**14-402E, ≤10 mg/dl; 14-490E, 14-491E, 14-498E, ≤25 mg/dl
290
Continued
Cell Growth Promotion – All serum lots of animal origin Chlamydia Functional Testing—Fetal bovine serum (Cat.
are tested for their growth promoting properties using No. 14-902E) labeled for chlamydia isolation is tested in
primary, diploid, and heteroploid cell lines unless other a controlled functional test. Dilutions of LGV1 or LGV2 are
cells are appropriate based on the product’s use. Results isolated in McCoy or L929 cells. Cultures are stained with
are obtained using the Lowry protein method to assess Jones Iodine for inclusion counts which must be >75% of
cell replication. Visual screening of cultures is also done to the control.
ensure characteristic cell morphology and lack of toxicity.
Origin and Types of Sera
Chemistry – pH, osmolality, total protein and hemoglobin Fetal Bovine Serum, Premium, US Origin
determinations are performed for each lot of serum. All whole fetal bovine serum is aseptically collected using a
Osmolality is determined by means of the highly repeatable cardiac puncture technique from USDA inspected abattoirs
freezing point method. Total protein is determined by the located within the 48 contiguous United States. Each lot
Biuret method. An extensive panel of chemical tests, is completely traceable back to the USDA establishment
including a biochemical profile, is run on each lot of animal number from which the raw material was obtained. Donor
serum. The biochemical profile includes: animals receive ante- and post-mortem inspection by an
Albumin Gamma Globulin SGOT on-site USDA veterinary inspector and appear to be free of
Alkaline Phosphatase Glucose SGPT infectious diseases.
Blood Urea Nitrogen Glutamyl Transpeptidase Sodium
Calcium % Gamma Globulin Total Bilirubin Fetal Bovine Serum, USDA Approved Source
Chloride Iron Total Protein All whole fetal bovine serum is aseptically collected using a
Cholesterol Lactic Dehydrogenase Triglycerides cardiac puncture technique from USDA approved sources.
Creatinine Phosphorus Uric Acid Each lot produced is safety tested for exotic viruses and
Free Fatty Acids Potassium
approved for importation into the United States by the
USDA. Donor animals receive ante- and post-mortem
Serum Identity – Samples are tested for characteristic
inspection by an on-site USDA veterinary inspector and
electrophoretic separation of proteins that appear
appear to be free of infectious diseases.
as visible bands or precipitate when the appropriate
antiserum is allowed to react with them. This technique Fetal Bovine Serum, Premium, US Origin, Irradiated
allows verification of serum speciation. Irradiated fetal bovine serum is produced in the same
manner and from the same high quality US origin fetal
bovine serum as our premium FBS and irradiated to reduce

Endotoxin – Serum products are routinely tested for the
presence of endotoxin. Testing is done by the Quantitative or destroy any bovine viruses. In addition, the premium
Chromogenic LAL method (Kinetic-QCL®) to yield a specific FBS is irradiated in its final product container, instead of
value reported in endotoxin units (EU/ml). Results of each in an unfinished state to assure that the effectiveness of
lot are available on the Certificate of Analysis issued for the irradiation process is not compromised due to further
each lot. product packaging after irradiation. Each lot must pass
complete growth promotion testing following irradiation
Immunoglobulin G – Fetal bovine serum is quantitatively to assure our customers that the irradiation treatment
tested by radial immunodiffusion. has not compromised the growth promoting abilities of
serum.
Bacteriophage – Fetal bovine serum lots are tested for the Calf Serum, (Bovine)
presence of bacteriophage. Whole bovine calf blood is of US origin and is collected from
animals that are 10 days to six months of age. Careful
Serum Tests for Special Applications handling and quick processing ensure that low levels of
endotoxin are maintained.
Hybridoma Support—Each new lot of fetal bovine
serum (Cat. No. 14-901) is labeled for use in hybridoma Calf Serum, Newborn (Bovine)
applications and is evaluated for its ability to support the Whole newborn calf blood is of US origin and is collected
growth of hybridoma cells. from animals that are 0 – 10 days of age. Newborn calf
serum is a popular alternative to fetal bovine serum.

291
Continued
Origin and Types of Sera Continued Handling Serum Products

Horse Serum, Donor – US Origin Animal serum varies from lot-to-lot. To minimize the
Whole donor horse blood is collected from a donor herd variability, it must be handled consistently.
located in the United States. Donors are fasted prior to
Receipt – All serum is stored frozen and shipped with dry
bleeding to reduce lipid concentration. Each animal
ice. A fee will be added to your invoice for dry ice packing.
receives routine Coggins tests for equine infectious
It is critical that all boxes containing serum be emptied
anemia (EIA). upon receipt.
Human Serum – US Collection Storage – We recommend that serum be stored at its
Whole human blood is obtained from normal human labeled temperature.
donors who test negative for Hepatitis B surface antigen
(HBsAg), antibodies to Hepatitis C (HCV), and Human Using Serum – Although the product has been sterile
filtered, aseptic procedures must be followed at all
Immunodeficiency Virus 1 and 2 (HIV-1 and HIV-2). Serum
times. Whenever possible use a biological safety cabinet
is obtained off the clot, centrifuged to remove red blood
that has been certified according to the National Sanitation
cells, pooled and sterile filtered. Foundation Standard 49.
Human Serum, AB – US Collection Granules, flocculent material or turbidity may develop
Whole human AB blood is obtained from normal human after thawing. This particulate matter does not alter
donors who test negative for Hepatitis B surface antigen the performance of the serum as a supplement for cell
(HBsAg), antibodies to Hepatitis C (HCV), and Human culture medium. Repeated freezing/thawing of serum
may increase the amount of precipitate and is therefore
Immunodeficiency Virus 1 and 2 (HIV-1 and HIV-2). Serum
not recommended. If you do not intend to use an entire
is obtained off the clot, centrifuged to remove red blood
bottle of serum within 2 freeze/thaw cycles, aliquot it into
cells, pooled and sterile filtered. usable quantities in sterile containers before freezing a
second time.
Human Serum, AB (Male only) – US Collection
Whole human AB blood is obtained from normal human Wipe the outside of each bottle with a disinfectant
male donors who test negative for Hepatitis B surface solution, proven effective against the normal bioburden
antigen (HBsAg), antibodies to Hepatitis C (HCV), and in your laboratory, before setting it on the work surface.
Remove the heat seal and wipe the outside of the cap with
Human Immunodeficiency Virus 1 and 2 (HIV-1 and HIV-2).
the disinfectant solution.
Serum is obtained off the clot, centrifuged to remove red

blood cells, pooled and sterile filtered.
Inactivation of Viruses and Mycoplasma by Gamma
Irradiation
Human Serum, AB (Male only), Plasma Derived –
US Collection As commercialization of biological products continues to
Human AB serum, derived from plasma, is obtained from escalate, regulatory agencies are looking more closely at
normal human male donors who test negative for Hepatitis B ingredients of animal origin being used in the manufacture
surface antigen (HBsAg), antibodies to Hepatitis C (HCV), of biological products.
and Human Immunodeficiency Virus 1 and 2 (HIV-1 and To satisfy the regulatory concerns our clients face, we
HIV-2). Serum is pooled and sterile filtered. have performed a study on the efficiency of using gamma
irradiation to remove bovine viruses from serum. Our
Serum Supreme, Iron Supplemented Calf Serum process of gamma irradiation delivers a precise dose of
Our affordable FBS alternative is of US Origin and supports a gamma irradiation to each bottle by multi-dimensional
wide range of cell types. dose mapping and standardized handling and packaging
configurations.
Our validation study includes demonstrating up to a
six-log reduction in bovine viruses, as well as confirming
the serum’s ability to support cell growth after irradiation.
The complete validation study is available to review during
an on-site audit of Lonza Walkersville, Inc.
292
Continued
FBS, US Origin, Irradiated (14-471) is routinely tested for Heat Inactivation of Serum
the presence of bovine viruses by full compliance 9CFR We recommend the following protocol for heat inactivation:
test procedures. These results include test results for the 1. Place the serum at room temperature for 3 – 4 hours
following bovine viruses: Bovine Viral Diarrhea (BVD), IBR before thawing the serum completely in a 37°C water
(CPE), PI3 (hemadsorption), Bovine parvovirus, Bovine bath.
adenovirus 3 and 5, Rabies virus, Blue tongue, Bovine
syncitial respiratory virus (BRSV) and Reovirus. 2. Preheat a water bath to 56°C.
3. Agitate the bottles to mix the contents. Set them into
In addition, each lot receives our full cell growth test the water bath so that the water level is above serum
following irradiation and a Certificate of Irradiation is level.
provided confirming the product received the proper dose 4. Place a thermometer in a bottle with an equivalent
required to inactivate bovine viruses. volume of water in the water bath as a control.
Special Serum Treatments 5. Mix the serum occasionally as the temperature rises.
Monitor the temperature of the water bath to ensure
We will heat inactivate or gamma irradiate serum on an that it stays at 56°C. When the temperature of the
individual basis. Customers may designate approved lots water in the control bottle reaches 56°C, set a timer
to be heat inactivated or gamma irradiated by informing for 30 minutes.
customer service when placing an order. There will be an 6. At regular intervals, ensure that the temperature is
additional charge for these services. Please allow 4 weeks or 56°C and swirl the bottles.
more, for these procedures.
7. At the end of 30 minutes, remove the bottles from the
Special Handling Fees water bath and label them as “heat inactivated”.
NOTE: Unless there is a compelling reason to heat inactivate your FBS, we
Heat Inactivation Irradiation recommend that it not be done. Heat inactivation was historically done for
many reasons that are no longer valid. Generally, FBS performs better in
$3.00/2,50 € per 50 ml $11.00/9,00 € per 50 ml cell culture without heat treatment.
$3.00/2,50 € per 100 ml $11.00/9,00 € per 100 ml
$6.00/5,00 € per 500 ml $11.00/9,00 € per 500 ml
$6.00/5,00 € per 1,000 ml $11.00/9,00 € per 1,000 ml
Minimum Charge: Minimum Charge:

$10.00/8,50 € $300.00/250,00 €
NOTE: Please contact your local Lonza office or your local distributor for
pricing outside the US or Europe.
293
Fetal Bovine Sera
Fetal Bovine Serum Premium, US Origin Fetal Bovine Serum Premium, US Origin, Heat Inactivated
Screened for viruses by full compliance 9CFR (113.53) and the absence of Heat inactivated at 56°C for 30 minutes; screened for viruses by
mycoplasmas; 3 × 0.1-micron filtered full compliance 9CFR (113.53) and the absence of mycoplasmas;
Storage Conditions: -10°C – -20°C 3 × 0.1-micron filtered
14-501E 100 ml
14-503E 100 ml
14-501F 500 ml
14-503F 500 ml
Fetal Bovine Serum, USDA Approved Source
Screened for viruses by full compliance 9CFR (113.53)and the absence of Fetal Bovine Serum Premium, US Origin,
mycoplasmas; 3 × 0.1-micron filtered
Hybridoma Screened
Screened to support hybridoma growth; screened for viruses by
full compliance 9CFR (113.53) and the absence of mycoplasmas;
14-502E 100 ml 3 × 0.1-micron filtered
14-502F 500 ml Storage Conditions: -10°C – -20°C
14-901E 100 ml
Fetal Bovine Serum, USDA Approved Source, 14-901F 500 ml
Heat Inactivated
Heat inactivated at 56°C for 30 minutes; screened for viruses by full
compliance 9CFR (113.53) and the absence of mycoplasmas; 3 × Fetal Bovine Serum Premium, US Origin, for Testing
0.1-micron filtered Chlamydia
Storage Conditions: -10°C – -20°C Screened to support chlamydia growth; contains 10X L-glutamine and
10X nystatin, a chlamydia isolation component; screened for viruses
14-507E 100 ml by full compliance 9CFR (113.53) and the absence of mycoplasmas;
14-507F 500 ml 3 × 0.1-micron filtered
Fetal Bovine Serum Premium, US Origin, Irradiated 14-902E 100 ml

Irradiation dose is 25 – 40 kGy; screened for viruses by full compliance
9CFR (113.53) and the absence of mycoplasmas; 3 × 0.1-micron filtered
14-471E 100 ml
14-471F 500 ml
Fetal Bovine and
Equine Sera
Equine Sera
Cat. No. Size Price
Horse Serum, Donor, US Donor

Screened for viruses by full compliance 9CFR (113.53) and the absence of
14-403E 100 ml
14-403F 500 ml
294
Bovine Sera

Calf Serum, US Origin Calf Serum, Newborn, US Origin

Bovine, screened for viruses by full compliance 9CFR and the absence of Bovine, screened for viruses by full compliance 9CFR and the absence of
mycoplasmas; 3 × 0.2-micron filtered mycoplasmas; 3 × 0.2-micron filtered
Storage Conditions: -10°C – -20°C Storage Conditions: -10°C – -20°C
14-401E 100 ml 14-416E 100 ml (US origin)

14-401F 500 ml 14-416F 500 ml (US origin)
DE14-417E 100 ml (Australian origin)
DE14-417F 500 ml (Australian origin)
Serum Supreme, Iron Supplemented Calf Serum

US Origin; screened for viruses by full compliance 9CFR and the absence of
14-492E 100 ml
14-492F 500 ml
Human Sera
Cat. No. Size Price
Human Serum
Bovine and Human Sera

14-402E 100 ml
Human Serum, AB
14-490E 100 ml
Human Serum, AB (Male only)

14-498E 100 ml
Human Serum, AB (Male only), Plasma Derived

14-491E 100 ml
Special Handling Instructions: Treat as potentially infectious. Each serum/

plasma donor unit used in the preparation of this product has been tested
by an FDA approved method and found non-reactive for the presence of
HBsAg, Hepatitis C, and antibody to HIV-1, and HIV-2. No known test method
can offer complete assurance that Hepatitis B virus, Hepatitis C virus, HIV-1,
HIV-2 or other infectious agents are absent. The serum/plasma is also tested
for the absence of mycoplasma. All human blood-based products should be
handled in accordance with currently acceptable biosafety practices and
guidelines for the prevention of blood-borne viral infections.
295
Fetal Bovine Serum (Available Outside USA and Canada)
The following sera are ONLY available for customers outside the USA and Canada
All sera listed follow EC regulations on import for serum Ordering Information
into the European community. Products manufactured
Cat. No. Size Price
using those sera might be subject to import restrictions
in some countries (e.g. USA). Fetal Bovine Serum, EU Standard (Low IgG, IgG content
lower than 100 microgram/ml)
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and
The EC approved fetal bovine sera are manufactured in a the absence of mycoplasma, 0.2 µm filtered
European ISO 9001 certified facility. Storage Conditions: -10°C – -20°C
DE14-870E 100 ml
■ Finished Product Quality Control
DE14-870F 500 ml
Samples of each lot of finished product are selected and
tested in accordance with written product specifications. Fetal Bovine Serum, EU Standard (Delipidized)
Final product quality control includes the following: the absence of mycoplasma, 0.2 µm filtered
– Sterility – Tests are performed on representative DE14-840E 100 ml
samples using a modification of the membrane DE14-840F 500 ml
filtration method described in the EP Pharmacopoeia
– Mycoplasma – Final products are tested for myco- Fetal Bovine Serum, EU Standard (ES qualified serum)
plasma using the culture test described European the absence of mycoplasma, 0.2 µm filtered
Pharmacopoeia (EP)
– Adventitious Viruses – All fetal bovine serum lots receive DE14-850E 100 ml
testing for adventitious viral agents including bovine DE14-850F 500 ml
viral diarrhea (BVD), infectious bovine rhinotracheites

(IBR) and parainfluenza virus type 3 (PI3)
– Cell Growth Promotion – All fetal bovine serum lots
are tested for their growth promoting properties using
MRC5 and L929 cell lines
– Chemistry, serum identity, endotoxin and immuno-
globulin testing is equal to that described in the
Outside USA and Canada
section for US manufactured sera

Fetal Bovine Sera
■ Applications:
– Support growth of undifferentiated embryonic stem
cells
– Low unspecific stimulation
296
Fetal Bovine Serum (Available Outside USA and Canada)
Continued

Fetal Bovine Serum EU Standard (UltraLow IgG, Fetal Bovine Serum EU Standard, Dialyzed
<8 mg/ml) the absence of mycoplasma. Dialyzed against physiological NaCl solution
South American origin (Brazil), screened for viruses (IBR, PI3 and BVD) and or PBS using a 10 kDa molecular weight cut-off membrane until the glucose
the absence of mycoplasma, 0.2 µm filtered level is < 10 mg/dl. To prevent precipitation and inactivation of serum peptides
Storage Conditions: -10°C – -20°C we do not perform exhaustive dialysis. Thus we do not certify that all low
molecular weight dialyzable components, such as amino acids, are totally
DE14-860F 500 ml removed by our processing. The dialyzed serum is sterile filtered using
0.2 µm filter.
Fetal Bovine Serum EU Standard
DE14-810E 100 ml
the absence of mycoplasma, 0.2 µm filtered
Storage Conditions: -10°C – -20°C DE14-810F 500 ml
DE14-801E 100 ml
Fetal Bovine Serum EU Standard, Charcoal Dextran
DE14-801F 500 ml
Treated
Fetal Bovine Serum EU Standard, Research Grade the absence of mycoplasma, Charcoal Dextran treated, 0.2 µm filtered
South American origin, screened for viruses (IBR, PI3 and BVD) and the absence Storage Conditions: -10°C – -20°C
of mycoplasma, 0.2 µm filtered
Storage Conditions: -10°C – -20°C DE14-820E 100 ml
DE14-820F 500 ml
DE14-802E 100 ml
DE14-802F 500 ml
Fetal Bovine Serum EU Standard, Tetracycline Free
Fetal Bovine Serum, USDA Country of Origin the absence of mycoplasma, 0.2 µm filtered
Australian origin, screened for viruses (IBR, PI3 and BVD) and the absence Storage Conditions: -10°C – -20°C
of mycoplasma, 0.2 µm filtered
Storage Conditions: -10°C – -20°C DE14-830E 100 ml
DE14-830F 500 ml
DE14-701E 100 ml
DE14-701F 500 ml
Outside USA and Canada

Fetal Bovine Sera
297
Cell Culture Technical Information
We offer an extensive line of cell culture media and of the commonly asked questions relating to cell culture
reagents backed by years of experience and innovation. techniques by providing instructions and tips for adapting
Through our own internal efforts as well as work with cultures to serum-free medium, cryopreservation and
exceptional collaborators, we are able to provide ongoing reconstitution, preparing powdered media, and more. Our
technical support in the form of protocols, detailed product Scientific Support Department is prepared to assist you
information, and troubleshooting tips for our broad range with many other technical questions and concerns related
of media and reagents. In this section we address many to cell culture and media.
Adaptation of Cell Cultures to Serum-free Medium

The conversion of a particular cell or cell line from growth cells are converted to a serum-free environment. However,
in serum-containing medium to serum-free medium is each investigator should monitor the cellular function of
achieved through the weaning process. However, weaning interest to their application during the weaning process.
is not required for all cell types. Rapid conversion of a cell
population to serum-free conditions can be achieved by We recommend two protocols for the conversion of
pelleting the cells and resuspending them in the serum- cell populations to a serum-free environment. These
free medium. While this may be successful for some protocols may be used for mammalian and invertebrate
types of cells, a gradual conversion is more likely to yield cell types. The first protocol may be used with attachment
the desired result. independent cells or cells that are loosely adherent and
do not require trypsinization. It involves the gradual
Weaning is actually a process by which a subpopulation dilution of the serum-containing medium with serum-
of cells that can proliferate in the absence of serum is free medium. The second protocol may be used with both
selected. The degree of difficulty in selecting these cells is attachment dependent and independent cell types and
a function of the physical and nutritional requirements of begins with the serum-free medium supplemented with
the cells and the complexity of the serum-free formulation. serum. A gradual reduction in the serum concentration is
Conversion of cells to growth in UltraCULTURE™ Medium can performed at each subculture until serum-free growth is
be relatively simple because it is a complex formula. Other achieved. This latter protocol has the added advantage of
formulations may contain reduced amounts of protein (i.e., establishing the limit of serum concentration for the cell
UltraCHO™ Medium and UltraDOMA™ Medium) or be entirely type. Some cells (especially transfected lines) require
devoid of proteins and peptides (i.e., UltraDOMA-PF™ small amounts of serum (i.e., 0.1 – 0.5% v/v). This method
Medium). In practice, these formulations require slightly allows the investigator to titrate the serum to the lower
more attention during the weaning process. However, the limit.
benefits of a low protein serum-free growth environment
and subsequent reduction in downstream processing The two weaning protocols are presented on the following
procedures more than offset the extra time spent in the page. They represent our recommended procedures,
weaning process. however, each investigator may choose to make
modifcations that better suit their particular application.
Maintenance of cellular function is an aspect of the In our experience, the minimum cell density maintained
weaning process that must be monitored. One needs to during the conversion process has a major effect on the
Adaption of Cell Cultures
to Serum-free Medium
ensure that the subpopulation selected exhibits the same outcome. We recommend that the cells be maintained
characteristics with respect to cellular function as the above 3.0 × 105/ml for attachment independent and above
population that was cultivated in the presence of serum. 30% confluency for attachment dependent cells.
These functions are diverse and may include receptor
expression, viral susceptibility, monoclonal antibody
production, and recombinant gene expression. In many
cases, an increase in product yield has been noted when
298
Protocols for Weaning Cell Cultures
Protocol #1: Medium Replacement – for adherent Protocol #2: Serum Dilution – for adherent dependent
independent cells (suspensions) cells
Approximate time required: 2 weeks – 6 weeks Approximate time required: 2 weeks–6 weeks
Culture conditions: Culture conditions:
– Mammalian cells: 95% air, 5% CO2, 35°C – 37°C – Mammalian cells: 95% air, 5% CO2, 35°C – 37°C
– Invertebrate cells: air, 25°C – 27°C – Invertebrate cells: air, 25°C – 27°C
1. Begin with cultures at maximum cell density. 1. Begin with cultures at maximum cell density.
note: Attachment dependent cells that are exposed to trypsin during
subculturing should be converted to serum-free growth using Protocol #2.
2. Trypsinize attachment dependent cultures and
transfer to an appropriately sized centrifuge tube.
2. Split cells 1:2 using serum-free medium as the Attachment independent cells may be transferred
diluent. directly to the centrifuge tube.
3. Incubate cells until the maximum cell density is 3. Sediment the cells by centrifugation at 350 × g for
achieved. 5 minutes.
4. Split cells 1:5 or to 3.0 × 105 cells/ml for attachment 4. Resuspend the cells in serum-free medium containing
independent cells or 30% confluency for attachment 5% serum (v/v).
dependent cells using serum-free medium as the
diluent. 5. Adjust the cell concentration using the serum
supplemented serum-free medium to a maximum of
5. Incubate cells until the maximum cell density is 3.0 × 105 cells/ml for attachment independent cells or
achieved. a density to achieve not less than 30% confluency for
6. If the cell viability is >85% at this point, and the attachment dependent cells.
generation time is similar to that observed with serum- 6. Plant the cells and incubate until a maximum cell
containing medium, the culture may be maintained in density is achieved.
serum-free medium using a similar split schedule as
originally optimized for serum-containing medium. 7. Repeat steps 2 – 6 using a lower concentration of
serum at each split. We recommend beginning at 5%
7. If the cells exhibit slow growth or low viability, maintain serum and lowering to 2%, 1%, 0.5%, and finally 0.1%
the split ratio at 1:2 or 1:5 for 3 successive splits. The prior to eliminating serum from the culture.
minimum cell density should be above 3.0 × 105 cells/ml
or 30% confluency during this period. note: If the culture viability drops below 80% or if the generation time
increases markedly following a decrease in the serum concentration,
8. Gradually increase the split ratio to obtain a maximum increase the serum level to the previous value and maintain the cells for
2 split cycles before lowering the level of serum again. It may be necessary
value for the cell type being used. to institute a more gradual decline in serum concentration with these cells.
Some cell types may require a small amount of serum for growth. If the
note: Some cells may require a small amount of serum for growth. If the cells have not adapted to serum-free cultivation using the protocol described
cells have not adapted to serum-free cultivation using the above protocol, above, add 0.1 – 0.5% serum to the culture or contact Scientific Support at
add 0.1% – 0.5% serum to the culture or contact Scientific Support at scientific.support.eu@lonza.com.
scientific.support.eu@lonza.com.
Protocols for Weaning
Cell Cultures
299
Cryopreservation and Reconstitution
Basic Procedure for Cryopreservation and Reconstitution Ampoule Handling Recommendations

of Cultured Cells
Receipt of Ampoule
note: Not applicable to Clonetics® and Poietics® Primary Human or Upon receipt, transfer the ampoule(s) from the shipping
Animal Cells. container to a -70°C freezer or a vapor phase nitrogen
Cryopreservation: tank. For long-term storage (over 3 months), a vapor
1. Select a flask of cells at or near confluency. phase nitrogen tank is preferable to prevent significant
2. Cells should be first removed by trypsinization. loss of viability. Immersion of screw cap ampoules in liquid
3. Adjust the cell concentration to between 2 × 106 nitrogen is not recommended.
and 8 × 106 cells/ml with EMEM (Cat. No. 12-136)
containing 20% Fetal Bovine Serum (Cat. No. 14-501). To use:
caution: Wear protective facemask and clothing as ampoule explosions can
Centrifugation may be necessary. occur.
4. Add the above cell suspension to an equal volume of 1. Remove an ampoule from the freezer and place it into
cold (+4°C) Cryoprotective Freezing Medium (Cat. No. a 37°C waterbath. Do not submerge the ampoule or
12-132A). allow water to get under the cap.
5. Mix continuously to ensure homogeneity. 2. After thawing, disinfect the ampoule with 70%
isopropanol, then open aseptically in a laminar flow
6. Dispense either 4 ml of the cells suspended in safety cabinet. Dilute the contents 1:10 in the appropriate
Cryoprotective Freezing Medium into 5 ml glass growth medium.
ampoules, or 1 ml into plastic screw cap vials suitable
for freezing in the liquid or vapor phase of liquid 3. Determine the viability and cell concentration of the
nitrogen. thawed cells by using the Trypan Blue exclusion cell
counting method.
7. Cells are now ready for the freezing cycle. Cells should
not be allowed to remain in the Cryoprotective Freezing 4. Adjust the cell concentration as desired for seeding
Medium for more than 1 hour before freezing. culture vessels.
8. The temperature of the contents in the ampoule 5. Twenty-four hours after cells have been seeded, remove
must then be lowered at a rate of 0.5°C – 2°C/minute the medium and refeed with the appropriate growth
throughout the range of +4°C to -30°C. medium. This will remove the cryopreservative, if the
alternative method described below is not used.
9. After the temperature has reached -30°C, the rate of
the temperature drop to -70°C (which is the warmest 6. As an alternative, the cryopreservative may be removed
temperature at which cells can be stored) can be prior to cell viability determination by centrifugation.
done very quickly. An automatic programmable This is done by centrifuging the resuspended cells at
freezer system is the most reliable means of obtaining low speed for 10 minutes (200 × g). The supernatant is
controlled rate freezing. removed and the cells are resuspended in the appropriate
growth medium.
10. Storage of the ampoules or vials must be at -70°C
or colder. A storage temperature of -196°C (liquid
nitrogen) is best. It is essential that the temperature Reference
of the contents of the ampoule be -70°C or colder Freshney, R.I. (2000) Culture of Animal Cells: A Manual of Basic Technique,
4th edition, Wiley-Liss, Inc., New York, pp. 297 – 308.
at all times until reconstitution. For prolonged or
indefinite storage, the use of liquid nitrogen is strongly
Cryoprerservation and
recommended. Storage in dry ice or a mechanical

Reconstitution
freezer (-70°C) should be limited to less than

3 months.
300
Determination of Cell Numbers
Counting cells by use of a hemacytometer is a convenient

and practical method of determining cell numbers in
suspension culture or from dispersed monolayer cultures.
The hemacytometer consists of two chambers, each of
which is divided into nine 1.0 mm squares. A cover glass
is supported 0.1 mm over these squares so that the total
volume over each square is 1.0 mm × 0.1 mm or 0.1 mm3,
or 10 –4 cm3. Since 1 cm3 is approximately equivalent to
1 ml, the cell concentration per ml will be the average
count per square × 104.
Hemacytometer counts are subject to the following

sources of error:
= count; = do not count
1. Unequal cell distribution in the sample.
Diagram of a hemacytometer, improved Neubauer ruling, 0.1 mm deep
2. Improper filling of chambers. Brackets indicate 1 mm2 squares. Circle is the approximate area covered at
100X magnification.
3. Failure to adopt a convention for counting cells in
contact with boundary lines or with each other. error is approximated by the square root of the total count.
4. Statistical error. A common convention is to count cells that touch the
middle line (of the triple lines) to the left and top of the
square, but not to count cells similarly located to the right
With careful attention to detail, the overall error can be and bottom (see diagram).
reduced to about 15%. It is assumed that the total volume
in the chamber represents a random sample. This will not In order to fill the hemacytometer chamber properly by
be a valid assumption unless the suspension consists capillary action, the cover slip, chamber, and the pipette
of individual separated cells. Cell distribution in the used to fill the chamber must be scrupulously clean. The
hemacytometer chamber depends on the particle number, chamber and cover slip are cleaned first with distilled
not particle mass. Thus, cell clumps will distribute the same water, then with absolute ethanol, and wiped dry.
as single cells and can distort the final result. Unless 90%
or more of the cells are free from contact with other cells, Hemacytometer counts do not distinguish between living
the count should be repeated with a new sample. Cells that and dead cells. A number of stains are useful to make
are difficult to obtain in uniform suspensions, or in which this distinction. Trypan blue, among others (erythrosin B,
extensive clumping cannot be avoided, may be counted nigrosin), is excluded by the membrane of the viable cells,
by separating nuclei. This method is more time-consuming whereas the nuclei of damaged or dead cells take up the
than direct counting and is subject to additional error if the stain. Although this distinction has been questioned, it has
population contains multinucleate cells. A sample will not the virtue of being simple and giving a good approximation.
be representative if the cells are permitted to settle before If more than 20% of the cells are stained, the result is
a sample is taken. Always mix the cell suspension thoroughly probably significant.
before sampling.
With a 10X objective and a 10X ocular, one square (1 mm2)

will approximately fill the microscope field (the circle on
the representation of a hemacytometer grid). The cell
Determination of
suspension should be diluted so that each such square

Cell Numbers
has between 20 and 50 cells (2 – 5 × 105 cells/ml). A total

of 300 to 400 cells should be counted since the counting
301
Determination of Cell Numbers
Continued
Materials Procedure
1. Clean hemacytometer and cover glass 1. Dilute 0.2 ml of Trypan Blue with 0.8 ml of HBSS.
2. Pasteur pipettes 2. Place cover glass over hemacytometer chamber.
3. Hanks’ Balanced Salt Solution (HBSS) 3. Transfer 0.5 ml of agitated cell suspension to a 12 × 75
(Cat. No. 10-543) mm tube and add 0.5 ml of diluted Trypan Blue.
4. Trypan Blue, 0.4% in BSS (Cat. No. 17-942E) 4. With Pasteur pipette, fill both chambers of the
5. Microscope hemacytometer (without overflow) by capillary action.
Cells will settle in the tube and in the pipette by gravity
6. Tubes (12 × 75 mm) within a few seconds. Work quickly.
7. Hand counter 5. Using a microscope with a 10X ocular and a 10X
8. Cell suspension objective, count the cells in each of 10 squares (1 mm2
each). If over 10% of the cells represent clumps, repeat
entire sequence. If fewer than 200 or more than 500
cells are present in the 10 squares, repeat with a more
suitable dilution factor.
6. Calculate the number of cells per ml, and total number of
cells in the original culture as follows:
Cells/ml = average count per square × 104 × dilution factor (i.e., 2, if 0.5 ml
of cells plus 0.5 ml of Trypan Blue is used)
Total cells = cells/ml × total volume of cell preparation from which sample
was taken
7. Repeat count to check reproducibility.
Determination of
Cell Numbers
302
Powdered Media Preparation
Preparation of Media for Filtration Procedure

Powdered media or salt mixtures are extremely 1. Select a suitable container as close in size to the final
hygroscopic and must be protected from atmospheric volume as possible. Measure out 90% of the final
moisture. The entire contents of each package should volume of deionized or distilled water (cell culture
be used immediately after opening. The preparation grade water is available from Lonza in volumes from
of medium in concentrated form is not recommended 500 ml to 200 L).
as some free-base amino acids have low solubility
coefficients and insoluble salt complexes may precipitate 2. While gently stirring, add the powdered medium or
in concentrated solution. All powdered products do not salt mixture. Continue stirring until dissolved. Do not
contain sodium bicarbonate, with the exception UltraCHO™ heat the water.
Media (Cat. No. 15-724). 3. Rinse the original package with a small amount of
deionized or distilled water. Add to solution.
Supplements can be added prior to filtration or introduced 4. For each liter of final volume of medium being prepared,
aseptically to sterile medium. Note: The nature of the add to the solution the required amount of sodium
supplement may affect the storage conditions and shelf
bicarbonate and/or L-glutamine (consult tables below).
life of the medium.
Continue stirring until completely dissolved.
5. While stirring, adjust the pH to 0.2 – 0.3 pH units
below the desired pH. Use 1 N HCl or 1 N NaOH. The pH
will normally rise 0.1 – 0.3 units during filtration.
6. Add additional deionized or distilled water to bring the
medium to final volume.
7. Sterilize immediately by filtration using a 0.22-micron
or smaller filter. To reduce the loss of CO2, positive
pressure (3 – 15 psi), with an inert gas (i.e., nitrogen)
should be used for filtering. The use of CO2 is not
recommended, as it will alter the pH of the medium.
8. After filtration, aseptically transfer into sterile
containers. Store medium at 2°C – 4°C in the dark until
ready to use.
Sodium bicarbonate addition table L-glutamine addition table
NaHCO3 NaHCO3 L-glutamine L-glutamine

Solution ml/L Powder g/L Solution ml/L Powder g/L
Product Product
Description Cat. No. 17-613 15-613 Description Cat. No. 17-605 15-605
DMEM w/L-glutamine 15-604 49.3 3.700 DMEM w/o L-glutamine 15-614 20.0 0.585
[4.0 mM] [4.0 mM]
DMEM w/o L-glutamine 15-614 49.3 3.700
MEM Eagle w/L-glutamine 15-611 29.3 2.200
Powered Media
Preparation
RPMI w/L-glutamine 15-702 26.7 2.000
303
Subculturing Procedures for Mammalian Cells
NOTE: Not applicable to Clonetics® and Poietics® Primary Human or Animal Trypsinization Procedure
Cells.
In cell culture there is frequently the need to subculture Cell cultures are normally subcultured (“split”) when the
cells. In doing so, cells can be propagated for the purposes cultures are at or near confluency. As a general rule, the
of increasing cell numbers or providing cells in a culture longer the time frame between when confluency is first
vessel suitable to one’s needs. There are a number of ways achieved and subculturing, the longer it will take for the
to remove cells from one culture vessel and pass them to trypsin to act.
another vessel. Cells may be removed from surfaces on 1. Decant medium from the culture vessel. Serum
which they are attached by: inhibits trypsin activity, so complete removal of
serum-containing medium is necessary.
1. Mechanical means (scraping)
2. Rinse the cell sheet with BSS without calcium
2. Chelating agents, ethylenediaminetetraacetic acid and magnesium before addition of Trypsin/Versene®
[EDTA] (Cat. No. 17-161). The monolayer should be thoroughly
3. Enzymes (trypsin, pronase, collagenase) covered with BSS. This rinse is instantaneous but the
Enzymes and chelating agents are often used in combi- BSS can remain on the cell sheet for up to 4 hours, if
nation. Trypsin is an aqueous crude extract prepared desired.
from porcine pancreas. It is the most common means 3. Pour off rinse medium. Trypsin/Versene® is to be added
used for removal of cells from surfaces and from intact to each vessel as follows:
tissue. Trypsin is, to some extent, a misnomer because 75 cm2 flask 2.5 ml to 5.0 ml
in addition to trypsin, the preparation contains other 150 cm2 flask 5.0 ml to 10.0 ml
proteases, lipases, and carbohydrases. The multitude 850 cm2 roller bottle 10.0 ml to 20.0 ml
of digestive enzymes produced by the pancreas would 4. Cover the monolayer thoroughly with Trypsin/Versene®.
be expected to be found in trypsin preparations. Pure Since different lots of Trypsin/Versene® may vary in
crystalline trypsin can be used, but it is more expensive strength, it is acceptable to monitor the trypsinization
than crude trypsin and often does not work as well, process at room temperature for the first 30 seconds.
especially when preparing cells from intact tissue. This will ensure that the trypsinization process is not
occurring too rapidly.
The optimum conditions for trypsin activity are a pH
range of 7.6 – 7.8 and a temperature of 37°C. The effect 5. The culture vessel should then be moderately hit
of trypsin is to break down the intracellular matrix that against the palm of the hand to see if the cells are
binds cells to each other or to a substrate surface. being dislodged. Hold the vessel up to a light in a
vertical position and look for signs of the cell sheet
There are no chemical standards for trypsin activity. We sloughing off of the surface. If the entire monolayer is
conduct quality assurance tests on trypsin to determine dislodged, proceed to step #6. If not, incubate at 37°C
its capacity to detach cells from a substrate surface in a and observe the vessel every minute for dissociation.
standard time period without damage. This is in addition The culture vessel should again be hit against the palm
to the usual tests for sterility. of the hand to ensure all cells have been dislodged.
Remove culture vessel from the incubator.
Trypsin is typically used at concentrations between 6. Immediately transfer dissociated cells to a vessel
0.05% and 0.25%, although some applications may containing medium supplemented with 10% serum. All
Subculturing Procedures
require concentrations outside this range. Versene® of the cells should be removed. Aspirate the medium
for Mammalian Cells
(EDTA) enhances trypsin action, and therefore lowers the plus cells with a pipette onto the surface to remove
required trypsin concentration for effective performance. all remaining cells. It is essential that this aspiration
Concentrated trypsin (2.5%, Cat. No. 17-160) should be be done as completely as possible with a small bore
diluted in calcium- and magnesium-free balanced salt pipette so as to obtain individual, dispersed cells. If the
solution (BSS) (Hanks’ BSS, Cat. No. 10-543; or Dulbecco’s cells are not separated, the new culture will contain
Phosphate Buffered Saline, Cat. No. 17-512). Dilution in numerous microcolonies. Cells added to the vessel
water is not recommended since the solution will be should be stirred with a magnetic stir bar at a speed
hypotonic and produce cell damage. Dilution in saline that avoids vortexing (approximately 100 – 200 rpm),
alone is also damaging to cells.
304
Subculturing Procedures for Mammalian Cells
Continued
Trypsinization Procedure, continued the number of population doublings that have elapsed.
or agitated frequently. It is important at this point to With a 1:2 split ratio this is achieved by simply adding
add medium containing serum at least 10 times the “1” to each split since this ratio yields one population
volume of Trypsin/Versene® used. This will ensure that doubling. Larger split ratios can be used. For example,
the digestive agent is inhibited. a split ratio of 1:4 would yield 2 doublings per 1:4 split;
a 1:8 split ratio would yield 3 doublings per 1:8 split. In
7. Add sufficient fresh medium to the aspirated order to have knowledge of the approach of cessation,
suspension so that the total volume will cover the it is essential to keep records of the number of elapsed
surface of two culture vessels, each having the same population doublings.
surface area as the original culture vessel (or use a
single culture vessel having twice the floor area of the 15. Since human diploid cells multiply by fission, the
original vessel). This is a 1:2 split. Other split ratios increase in population may be expressed per cell as
can be used for vigorously growing cell populations. follows:
1 2 4 8 16 …Number of cells
8. Incubate the culture vessel (or vessels) at 37°C. 0 1 2 3 4 …Population Doubling Level (PDL)
9. When making 1:2 splits, subculturing of human

diploid cell cultures should be done on a rigid 3 or 4 References
day schedule, at which time confluent sheets should 1. Hayflick, L. and Moorhead, P.S. (1961) The serial cultivation of human
diploid cell strains. Exp. Cell Research 25:585.
occur. Surplus cells can be frozen and stored in liquid
2. Hayflick, L. (1970) Aging under glass. Exp. Geront. 5:291.
nitrogen.
3. Hayflick, L. (1965) The limited in vitro lifetime of human diploid cell
10. Populations that can be cultivated indefinitely can be strains. Exp. Cell Res. 37:614.
subcultured serially each time confluency is reached. If 4. Hayflick, L. (1968) Human cells and aging. Scientific American 218:32.
the culture is a diploid population with a finite doubling 5. Hayflick, L. (1973) Subculturing human diploid fibroblast cultures.
Methods and Applications of Tissue Culture Eds. Patterson, M.K. and
capacity, increase the population doubling level (PDL) Kruse, P.F., Academic Press, N.Y.
number by one at each 1:2 subculturing (split). 6. Freshney, R.I. (1983) Culture of Animal Cells: A Manual of Basic Technique.
Alan R. Liss, Inc., New York.
11. By making repeated 1:2 splits (twice a week) it can be
seen that the number of culture vessels can be built
up geometrically (1, 2, 4, 8, 16, 32, 64, etc.) in a short
period of time for the production of large quantities of
cells for various purposes.
12. Although the line will be eventually lost as a
continuously passaged line, it will not be lost for use
since frozen ampoules can be obtained at almost
every passage and thus the line can be restored to
continuous passage again, up to a cumulative total
of about 50 population doublings. By repeating this
procedure, the number of cells that can be obtained is
almost unlimited for all practical purposes.
13. A human embryonic diploid line has an in vitro life
span of about fifty 1:2 subcultivations, or population
Subculturing Procedures
doublings, at which time the cells will cease to divide

for Mammalian Cells
and eventually die.

14. Using split ratios higher than 1:2 results in the
advantage of minimizing the number of manipulations
necessary to obtain a specific cell density or number
of culture vessels. Since human embryonic diploid
cell lines pass through a finite number of population
doublings in vitro, it is necessary to keep a record of
305
Cell Culture FAQs
Media FAQs
Q. Are all SFM (serum-free media) protein-free? Q. If medium is accidentally frozen, will it still
function properly?
A. No, this is a common misconception.
Q. What is chemically defined, serum-free medium? A. In most cases medium that is frozen should
not be adversely affected. Upon thawing the
A. Chemically defined media contain only medium should be examined for precipitate.
components of known molecular structure. If any evidence of precipitation is present, the
Constituents such as extracts or hydrolysates medium should be incubated in a 37°C water
are examples of undefined materials. bath for a few minutes. The bottles should be
gently swirled while in the water bath and the
Q. What are the advantages of using serum-free precipitate should go back into solution. If the
media? precipitate remains, the medium should be
A. – Reduces media variables discarded.
– Eliminates the expense and time required Q. Can you manufacture custom media formu-
to perform the extensive testing required by lations?
most labs to qualify new lots of serum A. Yes, Lonza provides custom media formulations
in bottles and bags of various sizes. If you
– Improves cell performance consistency
are interested in a custom production, please
– Eases downstream protein purification contact your sales representative.
Q. Does Lonza manufacture a serum-free medium Q. Why might customers want phenol red-free
that supports the growth of a wide variety of media?
cell lines?
A. Phenol red is a somewhat variable material
A. UltraCULTURE™ Medium (12-725F) is a complete shown to contain trace impurities that can mimic
serum-free medium designed for the cultivation the effects of estrogen. Some customers want
of a wide variety of mammalian cell types to avoid the risk of potential adverse impact of
including adherent and non-adherent primary these estrogen-like effects.
cells and established cell lines. Q. Can I expect comparable results using
Q. Can media products be used past their expiration ProFreeze™-CDM Medium as compared to
date? Why do certain media products not have serum-containing cryopreservation cocktails?
an expiration date? A. ProFreeze™-CDM Medium achieves these same
A. We do not recommend the use of a product past protective results, but with a chemically defined
its expiration date. Lonza has an ongoing shelf- formulation that contains no animal derived
life monitoring program that supports the labeled components. ProFreeze™-CDM Freezing Medium
expiration dates for its products. is universally suitable for cryopreserving many
cell types in the absence of fetal bovine serum
(FBS). However, it is used to greatest advantage
Products that are labeled for Research Use are with cells cultured in a serum-free and animal
non-In Vitro Diagnostic (IVD) products and are component-free environment. This protein-free
not required to be labeled with an expiration freezing medium contains no animal derived
date. Establishing appropriate shelf-life may components, insulin, or hydrolysate, and
be problematic because of the variety of maintains high cell viability upon recovery from
applications for which these products are used. frozen storage.
Lonza labels non-IVD products with the date of
Cell Culture FAQs
production so that the end user can calculate

an appropriate expiration date based on the
functionality of the medium for their particular
application.
306
Cell Culture FAQs
Continued
Sera FAQs
Q. The medium I purchased from Lonza already Q. What is the difference between Fetal Bovine
contains L-glutamine. Should I further supplement Serum (FBS)-USA and FBS-USDA?
the medium with additional L-glutamine prior to
A. FBS-USA are sera collected from USDA approved
use? sites within the United States. FBS-USDA are
A. Further supplementation of L-glutamine is not sera collected from USDA approved sites outside
necessary if the medium is used within expiration the United States.
period and stored according to product label. See Q. Is FBS tested for viruses?
the L-glutamine technical data sheet for more
information on the web at https://bcprd.lonza. A. Yes, all lots are full 9CFR 113.53 (c) [113.46,
com/shop/deeplink/area.do?area=1133510 113.47] tested for Bovine Viral Diarrhea (BVD),
Parainfluenza virus type 3 (P13), Bovine rhino
Q. What is the difference between L-glutamine and tracheitis (IBR), Bovine parvovirus, Bovine
UltraGlutamine? adeno 3, Bovine adeno 5, Rabies virus, Blue
A. UltraGlutamine is an extremely stable dipeptide tongue, Bovine respiratory syncytial virus
form of L-glutamine that avoids accumulation (BRSV), and Reovirus.
of toxic ammonia produced by spontaneous Q. What is the difference between calf serum and
degradation in the medium. Applications that newborn calf serum?
may benefit from UltraGlutamine are ammonia
sensitive systems (bioreactors), long-term A. Newborn calf serum is obtained from animals up
toxicity studies, and long incubations without to 10 days old. Calf serum is obtained from calves
feeding (cloning assays). 10 days to six months of age and weighing up to
200 kg.
Q. What are your endotoxin and hemoglobin
specifications for FBS?
A. We sell only Type I FBS. Our specifications for
FBS are endotoxin ≤10 EU/ml and hemoglobin
of ≤20 mg/deciliter. Typical endotoxin and
hemoglobin results of our FBS are undetected
and 6 – 9 mg/deciliter, respectively. Type II sera
have endotoxin and hemoglobin above these
threshold levels. Endotoxin and hemoglobin
levels exceeding these threshold levels are
indicative of poor handling and processing
practices. Endotoxin impacts over 30 biological
activities and can lead to cell death.
Q. Do I need to heat inactivate the fetal bovine
serum?
A. Unless you have a compelling scientific
reason to heat inactivate your FBS, we would
not recommend doing it. Heat inactivation was
historically done for many reasons that are no
longer valid. Generally, FBS performs better in
cell culture without heat treatment.
Cell Culture FAQs
307
Cell Culture FAQs
Continued
Sera FAQs Reagent FAQs

Q. Is your human sera derived from plasma or Q. Some suppliers report endotoxin in nanograms.
whole blood? How does that compare to EU (endotoxin units)?
A. We offer both. Most of our sera is "off-the-clot" A. One nanogram is equivalent to ten endotoxin
from whole blood. Whole blood is collected and units.
pooled. The blood is then allowed to clot and the
clot removed. The cells are removed and the sera Q. What working concentration do you suggest for
is then sterile filtered. your penicillin-streptomycin solutions?
We offer Human Serum, AB (Male only), 14-491E, A. Lonza offers various solutions with varying
derived from plasma through the process concentrations of penicillin-streptomycin. We
of plasmapheresis. Plasmapheresis collection suggest these solutions be diluted to 100 U
allows larger and more frequent donations penicillin/100 µg streptomycin for most cell
from a limited number of donors. Type AB male culture applications.
donors comprise less than one percent of the
population. Long-term or large volume needs Q. What is the concentration of your Trypsin-
for human AB serum are best addressed with Versene® (EDTA) mixture (17-161E, 17-161F)?
14-491E because plasma derived product is
more abundant. A. This product contains 0.5 g/L trypsin 1:250 and
0.2 g/L Versene® (EDTA) (0.05% Trypsin/0.02%
Q. Which human serum product will best meet my EDTA).
needs? Q. What working concentration do you suggest for
A. Immunological naiveté may be a factor in your gentamicin sulfate?
selecting the serum best suited to your needs.
Pooled sera from gender non-specific donors A. Lonza offers various solutions with varying
contain naturally occurring antibodies to ABO concentrations. For most applications, we
blood group antigens. Sera from blood type suggest these solutions be diluted to a working
AB donors do not contain naturally occurring concentration of 10 – 50 µg/ml.
antibodies to other blood group antigens. Q. What are the concentrations of the ITS (17-838Z)
Pools of gender non-specific sera also contain
antibodies raised in response to fetal antigens as and ITES (17-839Z) supplements?
a result of pregnancy. Sera from male AB donors A. Insulin – 5 mg/ml
lack antibodies attributed to pregnancy.
Transferrin – 5 mg/ml
Q. How can I be assured that your human sera Selenium – 5 µg/ml
products are of high quality?
Ethanolamine – 5 mM
A. Human blood or plasma is collected at FDA-
licensed facilities within the United States from
healthy normal individuals, pooled and sterile
filtered.
They are manufactured in strict compliance
with cGMP requirements.
Donors test non-reactive/negative using FDA-
approved methods for the following:
– Hepatitis B surface antigen (HBsAg)
– Antibodies to Hepatitis C (HCV)
Cell Culture FAQs
– Human Immunodeficiency Virus I (HIV-1 Ag)

– Antibodies to HIV-1 and HIV-2
– Syphilis
In addition, human sera products are QC tested
for sterility, pH, osmolality, mycoplasma, total
protein, and endotoxin.
308
Formulations for BioWhittaker® Classical Media
Basal Medium Eagle (BME)

Cat. No. 12-132 12-105
(Freezing Medium)
Liquid Liquid
COMPONENT mg/L mg/L
INORGANIC SALTS
CaCl2•2H20 158.00 265.00
KCl 340.00 400.00
KH2PO4 51.00
MgSO4•7H2O 170.00 200.00
NaCl 6,800.00 6,800.00
NaHC03 298.00 2,200.00
Na2HPO4•7H2O 77.00
NaH2PO4 (anhyd.) 140.00
OTHER COMPONENTS
Dimethylsulfoxide 150 ml
Glucose 850.00 1,000.00
Phenol Red•Na 17.00 10.00
AMINO ACIDS
L- Arginine•HCl 17.94 21.10
L -Cystine 10.20 12.00
L -Histidine•HCl•H20 8.93 10.50
L -Isoleucine 22.27 26.20
L -Leucine 22.27 26.20
L -Lysine•HCl 31.03 36.50
L -Methionine 6.38 7.50
L -Phenylalanine 14.03 16.50
L -Threonine 20.23 23.80
L -Tryptophan 3.40 4.00
L -Tyrosine 15.39 18.10
L -Valine 19.89 23.40
VITAMINS
d-Biotin 0.21 0.24
D-Ca Pantothenate 0.20 0.24
Choline Chloride 0.12 0.14
Folic Acid 0.38 0.44
Nicotinamide 0.10 0.12
Pyridoxine•HCl 0.17 0.20
Riboflavin 0.03 0.04
Thiamine•HCl 0.29 0.34 Formulations for
Classical Media
309
Continued
Dulbecco’s Modified Eagle's Medium (DMEM)

Cat. No. 12-604 15-604 12-614 15-614 12-707 12-708 12-709 12-733 12-741 12-914 12-917 BE12-604/U1
Liquid Powder Liquid Powder Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid
Component mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L
INORGANIC SALTS
CaCl2 (anhyd.) 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00
Fe(NO3)3•9H20 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10
KCl 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00
MgSO4 (anhyd.) 97.66 97.66
MgSO4•7H20 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00
NaCl 6,400.00 6,400.00 6,400.00 6,400.00 6,400.00 5,400.00 5,400.00 6,400.00 6,400.00 6,400.00 6,400.00 6,400.00
NaHC03 3,700.00 3,700.00 3,700.00 3,700.00 3,700.00 3,700.00 3,700.00 3,700.00 3,700.00 3,700.00
NaH2PO4 108.69 108.69
NaH2P04•H20 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00 125.00
OTHER COMPONENTS
Glucose 4,500.00 4,500.00 4,500.00 4,500.00 1,000.00 1,000.00 4,500.00 4,500.00 4,500.00 4,500.00 4,500.00 4,500.00
HEPES 5,957.50 5,957.50
Phenol Red 15.00 15.00 15.34 15.00 15.34 15.00 15.00 15.00 15.00 15.00 15.00
Sodium Pyruvate 110.00 110.00 110.00 110.00 110.00 110.00 110.00 110.00
AMINO ACIDS
L-Arginine•HCl 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00 84.00
L-Cystine 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00 48.00
L-Cystine•2HCl 62.58 62.58
L-Glutamine 584.00 584.00 584.00
L-Alanyl-L-Glutamine 868.00
(UltraGlutamine I)
Glycine 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00
L-Histidine•HCl•H20 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00
L-Isoleucine 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80
L-Leucine 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80 104.80
L-Lysine•HCl 146.20 146.20 146.20 146.20 146.20 146.20 146.20 146.20 146.20 146.20 146.20 146.20
L-Methionine 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00
L-Phenylalanine 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00 66.00
L-Serine 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00
L-Threonine 95.20 95.20 95.20 95.20 95.20 95.20 95.20 95.20 95.20 95.20 95.20 95.20
L-Tryptophan 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00 16.00
L-Tyrosine 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00 72.00
L-Tyrosine•2Na 103.79 103.79
L-Valine 93.60 93.60 93.60 93.60 93.60 93.60 93.60 93.60 93.60 93.60 93.60 93.60
VITAMINS
D-Ca Pantothenate 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
Choline Chloride 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
Folic Acid 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
i-Inositol 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00 7.00
Formulations for
Classical Media
Nicotinamide 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
Pyridoxine•HCl 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
Riboflavin 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40 0.40
Thiamine•HCl 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00 4.00
Dulbecco, R. and G. Freeman (1959) Virology 8:396 – 397.
310
Continued
DMEM: F-12 Ham’s Medium (F-12 and F-10)

Cat. No. 12-719 BE04-687 BE04-687/U1 Europe Only
Liquid Liquid Liquid Ham’s Ham’s Ham's
Component mg/L mg/L mg/L F-12 F-10 F-10
INORGANIC SALTS Cat. No. 12-615 12-618 BE02-014
CaCl2 (anhyd.) 116.00 116.00 116.00 Liquid Liquid Liquid
CuSO4•5H20 0.0012 0.0012 0.0012 Component mg/L mg/L mg/L
Fe(NO3)3•9H20 0.05 0.05 0.05 INORGANIC SALTS
FeSO4•7H20 0.42 0.42 0.42 CaCl2•2H20 44.11 44.10 44.10
KCl 311.83 311.83 311.83 CuSO4•5H20 0.0025 0.0025 0.0025
MgCl2•6H20 61.00 61.00 61.00 FeSO4•7H20 0.83 0.83 0.83
MgSO4•7H20 100.00 100.00 100.00 KCl 223.65 285.00 285.00
NaCl 6,999.50 6,999.50 6,999.50 KH2PO4 83.00 83.00
NaHCO3 1,200.00 1,200.00 1,200.00 MgCl2•6H20 122.00
Na2H2P04•7H20 134.00 134.00 134.00 MgSO4•7H20 152.80 152.80
NaH2P04•H20 62.50 62.50 62.50 NaCl 7,599.00 7,400.00 7,400.00
ZnSO4•7H20 0.43 0.43 0.43 NaHCO3 1,176.00 1,200.00 1,200.00
OTHER COMPONENTS Na2HPO4•7H20 268.10 290.00 290.00
Glucose 3,151.00 3,151.00 3,151.00 ZnSO4•7H20 0.86 0.29 0.29
HEPES 3,574.50 OTHER COMPONENTS
Hypoxanthine 2.04 2.04 2.04 Glucose 1,801.60 1,100.00 1,100.00
Linoleic Acid 0.04 0.04 0.04 Hypoxanthine 4.08 4.08 4.08
Lipoic Acid 0.10 0.103 0.103 Linoleic Acid 0.08
Phenol Red•Na 8.00 8.00 8.00 Lipoic Acid 0.21 0.20 0.20
Putrescine•2HCl 0.08 0.08 0.08 Phenol Red•Na 1.24 1.20 1.20
Sodium Pyruvate 110.00 110.00 110.00 Putrescine•2HCl 0.16
Thymidine 0.36 0.36 0.36 Sodium Pyruvate 110.00 110.00 110.00
AMINO ACIDS Thymidine 0.73 0.73
L-Alanine 4.46 4.46 4.46 AMINO ACIDS
L-Arginine•HCl 147.35 147.35 147.35 L-Alanine 8.91 8.91 8.91
L-Asparagine•H20 7.50 7.50 7.50 L-Arginine•HCl 210.70 211.00 211.00
L-Aspartic Acid 6.66 6.66 6.66 L-Asparagine•H20 15.01 15.00 15.00
L-Cysteine•HCl•H20 17.56 17.56 17.56 L-Aspartic Acid 13.31 13.30 13.30
L-Cystine 24.00 24.00 24.00 L-Cysteine•HCl•H20 35.12 35.13 35.13
L-Glutamic Acid 7.36 7.36 7.36 L-Glutamic Acid 14.71 14.70 14.70
L-Glutamine 365.10 365.10 L-Glutamine 146.20 146.20
L-Alanyl-L-Glutamine 868.00 L-Alanyl•L-Glutamine 446.00
(UltraGlutamine 1) Glycine 7.51 7.51 7.51
Glycine 18.76 18.76 18.76 L-Histidine•HCl•H20 20.96 21.00 23.00
L-Histidine•HCl•H20 31.48 31.48 31.48 L-Isoleucine 3.94 2.60 2.60
L-Isoleucine 54.37 54.37 54.37 L-Leucine 13.12 13.10 13.10
L-Leucine 58.96 58.96 58.96 L-Lysine•HCl 36.54 29.30 29.30
L-Lysine•HCl 91.37 91.37 91.37 L-Methionine 4.48 4.48 4.48
L-Methionine 17.24 17.24 17.24 L-Phenylalanine 4.96 4.96 4.96
L-Phenylalanine 35.48 35.48 35.48 L-Proline 34.53 11.50 11.50
L-Proline 17.27 17.27 17.27 L-Serine 10.51 10.50 10.50
L-Serine 26.26 26.26 26.26 L-Threonine 11.91 3.57 3.57
L-Threonine 53.56 53.56 53.56 L-Tryptophan 2.04 0.60 0.60
L-Tryptophan 9.02 9.02 9.02 L-Tyrosine 5.44 1.81 1.81
L-Tyrosine 38.72 38.72 38.72 L-Valine 11.71 3.50 3.50
L-Valine 52.66 52.66 52.66 VITAMINS
VITAMINS d-Biotin 0.007 0.024 0.024
d-Biotin 0.004 0.004 0.004 D-Ca Pantothenate 0.24 0.72 0.72
D-Ca Pantothenate 2.12 2.12 2.12
Formulations for
Choline Chloride 13.96 0.70 0.70

Classical Media
Choline Chloride 8.98 8.98 8.98 Folic Acid 1.32 1.32 1.32
Folic Acid 2.66 2.66 2.66 i-Inositol 18.00 0.54 0.54
i-Inositol 12.51 12.50 12.50 Nicotinamide 0.037 0.620 0.62
Nicotinamide 2.02 2.02 2.02 Pyridoxine•HCl 0.062 0.210 0.21
Pyridoxine•HCl 2.03 2.03 2.03 Riboflavin 0.038 0.380 0.38
Riboflavin 0.22 0.22 0.22 Thiamine•HCl 0.34 1.01 1.01
Thiamine•HCl 2.17 2.17 2.17 Vitamin B12 1.36 1.36 1.36
Vitamin B12 0.68 0.68 0.68
1. Ham, R.G. (1965) Proc. Natl. Acad. Sci. USA 53: 288 – 293.
2. Ham, R.G. (1963) Exp. Cell Res. 29:515 – 526.
311
Continued
Grace's Insect Medium Insect Medium

Cat. No. 04-457 04-649 04-501 Europe Only
Liquid Liquid Liquid Schneider's TC-100
COMPONENT mg/L mg/L mg/L Cat. No. 04-351 BE02-011
INORGANIC SALTS Liquid Liquid
CaCl2•2H2O 993.00 993.00 993.00 COMPONENT mg/L mg/L
KCl 4,100.00 4,100.00 4,100.00 INORGANIC SALTS
MgCl2•6H2O 2,280.00 2,280.00 2,280.00 CaCl2 (anhyd.) 600.00 996.66
MgSO4 (anhyd.) 1,358.00 KCl 1,600.00 2,870.00
MgSO4•7H2O 2,780.00 2,780.00 KH2PO4 450.00
NaHCO3 350.00 350.00 350.00 MgCl2 (anhyd.) 1,068.19
NaH2PO4•H2O 1,008.00 1,008.00 1,008.00 MgCl2•6H2O
OTHER COMPONENTS MgSO4 (anhyd.) 1,357.86
Fetal Bovine Serum 10%(v/v)(Heat MgSO4•7H2O 3,700.00
Inactivated)
MnCl2•4H2O 1,321.00
Fructose 400.00 400.00 400.00
NaCl 2,100.00
Fumaric Acid 55.00 55.00 55.00
NaHCO3 400.00 350.00
Gentamicin Sulfate 50.00 55.00
NaH2PO4•H2O 876.92
Glucose 700.00 700.00 700.00
OTHER COMPONENTS
α-Ketoglutaric Acid 370.00 370.00 370.00
Fumaric Acid 100.00
Lactalbumin Hydrolysate 3,330.00 3,330.00 Glucose 2,000.00 1,000.00
Malic Acid 670.00 670.00 670.00 200.00
α-Ketoglutaric Acid
Succinic Acid 60.00 60.00 60.00
L-Malic Acid 100.00
Sucrose 26,680.00 26,680.00 26,680.00
Succinic Acid 100.00
Yeastolate 3,330.00 3,330.00
Trehalose•2H2O 2,000.00
AMINO ACIDS
Tryptose Broth 2,600.00
L-Alanine 225.00 225.00 225.00
Yeastolate 2,000.00
ß-Alanine 200.00 200.00 200.00
AMINO ACIDS
L-Arginine•HCl 700.00 700.00 700.00
L-Alanine 225.00
L-Asparagine•H2O 398.00 398.00 398.00
ß-Alanine 500.00 700.00
L-Aspartic Acid 350.00 350.00 350.00
L-Arginine•HCl 350.00
L-Cystine•2HCl 28.29 28.29 28.29
L-Arginine•FB 400.00
L-Glutamic Acid 600.00 600.00 600.00
L-Asparagine 1,300.00 350.00
L-Glutamine 600.00 600.00 600.00
L-Aspartic Acid 400.00 350.00
Glycine 650.00 650.00 650.00
L-Cysteine 60.00
L-Histidine 2,500.00 2,500.00 2,500.00
L-Cystine 100.00 19.20
L-Isoleucine 50.00 50.00 50.00
L-Glutamic Acid 800.00 600.00
L-Leucine 75.00 75.00 75.00
L-Glutamine 1,800.00 600.00
L-Lysine•HCl 625.00 625.00 625.00
Glycine 250.00 650.00
L-Methionine 50.00 50.00 50.00
L-Histidine 400.00 2,500.00
L-Phenylalanine 150.00 150.00 150.00
L-Hydroxyproline 550.00
L-Proline 350.00 350.00 350.00
L-Isoleucine 150.00 50.00
DL-Serine 1,100.00 1,100.00 1,100.00
L-Leucine 150.00 75.00
L-Threonine 175.00 175.00 175.00
L-Lysine•HCl 1,650.00 625.00
L-Tryptophan 100.00 100.00 100.00
L-Methionine 800.00 50.00
L-Tyrosine•2Na•2H2O 72.08 72.08 72.08
L-Phenylalanine 150.00 150.00
L-Valine 100.00 100.00 100.00
L-Proline 1,700.00 350.00
VITAMINS
L-Serine 250.00 550.00
p-Aminobenzoic Acid 0.02 0.02 0.02
L-Threonine 350.00 175.00
d-Biotin 0.01 0.01 0.01
L-Tryptophan 100.00
D-Ca Pantothenate 0.02 0.02 0.02
L-Tyrosine 500.00
Choline Chloride 0.20 0.20 0.20
L-Tyrosine•2Na•2H2O 72.20
Folic Acid 0.02 0.02 0.02
L-Valine 300.00 100.00
i-Inositol 0.02 0.02 0.02
VITAMINS
Nicotinic Acid 0.02 0.02 0.02
p-Aminobenzoic Acid 0.02
Formulations for
Classical Media
Pyridoxine•HCl 0.02 0.02 0.02

d-Biotin 0.01
Riboflavin 0.02 0.02 0.02
D-Ca Pantothenate 0.02
Thiamine•HCl 0.02 0.02 0.02
Choline Chloride 0.20
Folic Acid 0.02
i-Inositol 0.02
Niacin 0.02
Pyridoxine•HCl 0.02
Riboflavin 0.02
Thiamine•HCl 0.02
312
Continued
Iscove’s Modified Dulbecco’s Medium (IMDM) L-15 (Leibovitz) Medium McCoy’s 5A Medium
Cat. No. 12-722 12-726 12-915 Cat. No. 12-669 12-700 Cat. No. 12-168 12-688
Liquid Liquid Liquid 2X Liquid Liquid Liquid Liquid
COMPONENT mg/L mg/L mg/L COMPONENT mg/L mg/L COMPONENT mg/L mg/L
INORGANIC SALTS INORGANIC SALTS INORGANIC SALTS
CaCl2 (anhyd.) 165.00 165.00 165.00 CaCl2(anhyd.) 280.00 140.00 CaCl2 (anhyd.) 100.00 100.00
KCl 330.00 330.00 330.00 KCl 800.00 400.00 KCl 400.00 400.00
KNO3 0.076 0.076 0.076 KH2PO4 120.00 60.00 MgSO4•7H2O 200.00 200.00
MgSO4 (anhyd.) 97.66 97.67 97.67 MgCl2 anhyd.) 187.34 93.67 NaCl 5,460.00 6,460.00
NaCl 4,505.00 4,505.00 4,505.00 MgSO4 (anhyd.) 195.46 97.70 NaHCO3 2,200.00 2,200.00
NaHCO3 3,024.00 3,024.00 3,024.00 NaCl 16,000.00 8,000.00 NaH2PO4•H2O 580.00 580.00
NaH2PO4•H2O 125.00 125.00 125.00 Na2HPO4 (anhyd.) 380.00 190.00 OTHER COMPONENTS
Na2SeO3•5H20 0.01 0.01 0.01 OTHER COMPONENTS Bactopeptone 600.00 600.00
OTHER COMPONENTS D(+) Galactose 1,800.00 900.00 Glucose 3,000.00 3,000.00
Glucose 4,500.00 4,500.00 4,500.00 Phenol Red•Na 10.00 Glutathione (reduced) 0.50 0.50
HEPES 5,958.00 5,958.00 5,958.00 Sodium Pyruvate 1,100.00 550.00 HEPES 5,957.40
Phenol Red•Na 15.00 15.00 15.00 AMINO ACIDS Phenol Red•Na 10.00 10.00
Sodium Pyruvate 110.00 110.00 110.00 DL-Alanine 900.00 450.00 AMINO ACIDS
AMINO ACIDS L-Arginine 1,000.00 500.00 L-Alanine 13.36 13.36
L-Alanine 25.00 25.00 25.00 L-Asparagine 500.00 250.00 L-Arginine•HCl 42.14 42.14
L-Arginine•HCl 84.00 84.00 84.00 L-Cysteine 240.00 120.00 L-Asparagine•H2O 45.03 45.03
L-Asparagine•H2O 28.40 28.40 28.40 Glycine 400.00 200.00 L-Aspartic Acid 19.97 19.97
L-Aspartic Acid 30.00 30.00 30.00 L-Histidine 500.00 250.00 L-Cysteine•HCl•H2O 35.12 35.12
L-Cystine•2HCl 91.24 91.24 91.24 DL-Isoleucine 500.00 250.00 L-Glutamic Acid 22.07 22.07
L-Glutamic Acid 75.00 75.00 75.00 L-Leucine 250.00 125.00 L-Glutamine 219.15 219.15
L-Glutamine 584.00 584.00 L-Lysine 150.00 75.00 Glycine 7.51 7.51
Glycine 30.00 30.00 30.00 DL-Methionine 300.00 150.00 L-Histidine•HCl•H2O 20.96 20.96
L-Histidine•HCl•H2O 42.00 42.00 42.00 DL-Phenylalanine 500.00 250.00 Hydroxy L•Proline 19.67 19.67
L-Isoleucine 105.00 105.00 105.00 L-Serine 400.00 200.00 L-Isoleucine 39.36 39.36
L-Leucine 105.00 105.00 105.00 DL-Threonine 1,200.00 600.00 L-Leucine 39.36 39.36
L-Lysine•HCl 146.00 146.00 146.00 L-Tryptophan 40.00 20.00 L-Lysine•HCl 36.54 36.54
L-Methionine 30.00 30.00 30.00 L-Tyrosine•2Na•2H2O 370.00 370.00 L-Methionine 14.92 14.92
L-Phenylalanine 66.00 66.00 66.00 DL-Valine 400.00 200.00 L-Phenylalanine 16.52 16.52
L-Proline 40.00 40.00 40.00 VITAMINS L-Proline 17.27 17.27
L-Serine 42.00 42.00 42.00 DL-Ca Pantothenate 2.00 1.00 L-Serine 26.28 26.28
L-Threonine 95.00 95.00 95.00 Choline Chloride 2.00 1.00 L-Threonine 17.87 17.87
L-Tryptophan 16.00 16.00 16.00 Folic Acid 2.00 1.00 L-Tryptophan 3.06 3.06
L-Tyrosine•2Na•2H2O 104.20 104.20 104.20 i-Inositol 4.00 2.00 L-Tyrosine 18.12 18.12
L-Valine 94.00 94.00 94.00 Nicotinamide 2.00 1.00 L-Valine 17.57 17.57
VITAMINS Pyridoxine•HCl 2.00 1.00 VITAMINS
d-Biotin 0.013 0.013 0.013 Riboflavin-5-PO4•Na 0.20 0.10 p-Aminobenzoic Acid 1.00 1.00
D-Ca Pantothenate 4.00 4.00 4.00 Thiamine 2.00 1.00 Ascorbic Acid 0.50 0.50
Choline Chloride 4.00 4.00 4.00 •Monophosphate d-Biotin 0.20 0.20
Folic Acid 4.00 4.00 4.00 Leibovitz:, A. (1963) Am. J. Hyg. 78: D-Ca Pantothenate 0.20 0.20
i-Inositol 7.20 7.20 7.20 173 – 180. Choline Chloride 5.00 5.00
Nicotinamide 4.00 4.00 4.00 Folic Acid 10.00 10.00
Pyridoxine•HCl 4.00 4.00 4.00 i-Inositol 36.00 36.00
Riboflavin 0.40 0.40 0.40 Nicotinamide 0.50 0.50
Thiamine•HCl 4.00 4.00 4.00 Nicotinic Acid 0.50 0.50
Vitamin B12 0.013 0.013 0.013 Pyridoxal•HCl 0.50 0.50
Iscove, N.N. and F. Melchers (1978) J. of Exptl.Med.147: Pyridoxine•HCl 0.50 0.50
923 – 933. Riboflavin 0.20 0.20
Thiamine•HCl 0.20 0.20
Vitamin B12 2.00 2.00
McCoy, T.A., M. Maxwell, and P.F. Kruse, Jr.
Formulations for
Classical Media
(1959) Proc. Soc. Exptl. Biol. Med. 100:

115 – 118.
313
Continued
Medium 199 Continued

Cat. No. 12-109 12-117 12-118 12-119 Cat. No. 12-109 12-117 12-118 12-119
Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid
COMPONENT mg/L mg/L mg/L mg/L COMPONENT mg/L mg/L mg/L mg/L
INORGANIC SALTS VITAMINS
CaCl2•2H2O 186.00 265.00 186.00 265.00 p-Aminobenzoic 0.05 0.05 0.05 0.05
Fe(NO3)3•9H2O 0.72 0.72 0.72 0.72 Acid
KCl 400.00 400.00 400.00 400.00 Ascorbic Acid 0.05 0.05 0.05 0.05
KH2PO4 60.00 60.00 d-Biotin 0.01 0.01 0.01 0.01
MgSO4•7H2O 200.00 200.00 200.00 200.00 Calciferol 0.10 0.10 0.10 0.10
NaCl 8,000.00 5,800.00 7,000.00 6,800.00 D-Ca Pantothenate 0.01 0.01 0.01 0.01
NaHCO3 1,400.00 2,200.00 1,400.00 2,200.00 Cholesterol 0.20 0.20 0.20 0.20
Na2HPO4•7H2O 90.00 90.00 Choline Chloride 0.50 0.50 0.50 0.50
NaH2PO4•H2O 140.00 140.00 Folic Acid 0.01 0.01 0.01 0.01
OTHER COMPONENTS i-Inositol 0.05 0.05 0.05 0.05
Adenine Sulfate 10.00 10.00 10.00 10.00 Menadione 0.01 0.01 0.01 0.01
Adenosine 0.20 0.20 0.20 0.20 Nicotinamide 0.025 0.025 0.025 0.025
Monophosphate Nicotinic Acid 0.025 0.025 0.025 0.025
Adenosine 1.00 1.00 1.00 1.00 Pyridoxal•HCl 0.025 0.025 0.025 0.025
Triphosphate•2 Na Pyridoxine•HCl 0.025 0.025 0.025 0.025
Deoxyribose 0.50 0.50 0.50 0.50 DL-Tocopheryl 0.01 0.01 0.01 0.01
Glucose 1,000.00 1,000.00 1,000.00 1,000.00 Phosphate Na2
Glutathione 0.05 0.05 0.05 0.05 Riboflavin 0.01 0.01 0.01 0.01
(reduced) Thiamine•HCl 0.01 0.01 0.01 0.01
Guanine•HCl 0.30 0.30 0.30 0.30 Vitamin A Acetate 0.14 0.14 0.14 0.14
HEPES 5,957.40 5,957.40
Morgan, J.F., H.J. Morton, and R.C. Parker
Hypoxanthine 0.30 0.30 0.30 0.30 (1950) Proc. Soc. Exptl. Biol. Med. 73:1 – 8.
Phenol Red•Na 15.00 15.00 15.00 15.00
Ribose 0.50 0.50 0.50 0.50
Sodium 83.00 83.00 83.00 83.00
Acetate•3H2O
Thymine 0.30 0.30 0.30 0.30
Tween 80 4.90 4.90 4.90 4.90
Uracil 0.30 0.30 0.30 0.30
Xanthine 0.30 0.30 0.30 0.30
AMINO ACIDS
L-Alanine 25.00 25.00 25.00 25.00
L-Arginine•HCl 70.00 70.00 70.00 70.00
L-Aspartic Acid 30.00 30.00 30.00 30.00
L-Cysteine•HCl•H2O 0.10 0.10 0.10 0.10
L-Cystine 20.00 20.00 20.00 20.00
L-Glutamic Acid 67.00 67.00 67.00 67.00
L-Glutamine 100.00 100.00 100.00 100.00
Glycine 50.00 50.00 50.00 50.00
L-Histidine•HCl•H2O 22.00 22.00 22.00 22.00
Hydroxy L•Proline 10.00 10.00 10.00 10.00
L-Isoleucine 20.00 20.00 20.00 20.00
L-Leucine 60.00 60.00 60.00 60.00
L-Lysine•HCl 70.00 70.00 70.00 70.00
L-Methionine 15.00 15.00 15.00 15.00
L-Phenylalanine 25.00 25.00 25.00 25.00
L-Proline 40.00 40.00 40.00 40.00
L-Serine 25.00 25.00 25.00 25.00
L-Threonine 30.00 30.00 30.00 30.00
L-Tryptophan 10.00 10.00 10.00 10.00
L-Tyrosine 40.00 40.00 40.00 40.00
Formulations for
Classical Media
L-Valine 25.00 25.00 25.00 25.00
314
Continued
Minimum Essential Medium (MEM)

Cat. No. 04-719 06-174 12-125 12-127 12-136 12-137 12-169 12-611 15-611 12-662 12-668
Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Powder Liquid Liquid 2X
Component mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L
INORGANIC SALTS
CaCl2•2H2O 265.00 186.00 265.00 186.00 265.00 265.00 264.87 265.00 530.00
KCl 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 800.00
KH2PO4 60.00 60.00
MgCl2•6H2O 200.00
MgSO4 (anhyd.) 97.67
MgSO4•7H2O 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 400.00
NaCl 6500.00 6800.00 6800.00 8000.00 5800.00 7000.00 6800.00 6800.00 6800.00 6800.00 13600.00
NaHCO3 2000.00 2200.00 2200.00 350.00 2200.00 350.00 2200.00 2200.00 2200.00 4400.00
NaH2PO4 (anhyd.) 121.74
NaH2PO4•H2O 1327.00 140.00 140.00 140.00 140.00 140.00 140.00 280.00
NaH2PO4•7H2O 90.00 90.00
OTHER COMPONENTS
Glucose 2000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 1000.00 2000.00
HEPES 5957.40 5957.50
Lipoic Acid 0.20
Phenol Red•Na 10.00 10.00 10.00 20.00 10.00 20.00 10.00 10.00 10.20 10.00
Sodium Pyruvate 110.00 110.00
AMINO ACIDS
L-Alanine 8.90 25.00 8.90
L-Arginine•HCl 126.00 126.40 126.40 126.40 126.40 126.40 126.40 126.40 126.98 126.40 252.80
L-Asparagine 13.21
L-Asparagine•H2O 13.21 50.00
L-Aspartic Acid 13.30 30.00 13.30
Cysteine•HCl•H2O 100.00
L-Cystine•2HCl 32.40 31.29
L-Cystine 24.00 24.00 24.00 24.00 24.00 24.00 24.00 24.00 48.00
L-Glutamic Acid 14.70 75.00 14.70
L-Glutamine 294.00 292.00 292.00 292.00
Glycine 7.50 50.00 7.50
L-Histidine, free base 31.00
L-Histidine•HCl•H2O 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 42.00 84.00
L-Isoleucine 52.00 52.40 52.40 52.40 52.40 52.40 52.40 52.40 52.00 52.40 104.80
L-Leucine 52.00 52.40 52.40 52.40 52.40 52.40 52.40 52.40 52.00 52.40 104.80
L-Lysine, free base 58.00
L-Lysine•HCl 73.00 73.00 73.00 73.00 73.00 73.00 73.00 72.46 73.00 146.00
L-Methionine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 30.00
L-Phenylalanine 32.00 33.00 33.00 33.00 33.00 33.00 33.00 33.00 32.00 33.00 66.00
L-Proline 11.50 40.00 11.50
L-Serine 10.50 25.00 10.50
L-Threonine 48.00 47.60 47.60 47.60 47.60 47.60 47.60 47.60 48.00 47.60 95.20
L-Tryptophan 10.00 10.20 10.20 10.20 10.20 10.20 10.20 10.20 10.00 10.20 20.40
L-Tyrosine 36.20 36.20 36.20 36.20 36.20 36.20 36.20 36.20 72.40
L-Tyrosine•2Na 51.90
L-Valine 46.00 46.80 46.80 46.80 46.80 46.80 46.80 46.80 46.00 46.80 93.60
VITAMINS
Ascorbic Acid 50.00
d-Biotin 0.10
D-Ca Pantothenate 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 2.00
Choline Chloride 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 2.00
Formulations for
Classical Media
Folic Acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 2.00
i-Inositol 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 2.00 4.00
Nicotinamide 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 2.00
Pyridoxal•HCl 1.00 1.00 1.00 1.00 2.00
Pyridoxine•HCl 1.00 1.00 1.00 1.00 1.00 1.00
Riboflavin 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.10 0.20
Thiamine•HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 2.00
Vitamin B12 1.33
315
Continued
Minimum Essential Medium (MEM) Continued

Cat. No. 12-684 12-739 BE02-002 BE02-020 Cat. No. 12-684 12-739 BE02-002 BE02-020
Liquid 10X Liquid Liquid Liquid Liquid 10X Liquid Liquid Liquid
COMPONENT mg/L mg/L mg/L mg/L COMPONENT mg/L mg/L mg/L mg/L
INORGANIC SALTS VITAMINS
CaCl2•2H2O 2,650.00 264.92 265.00 265.00 Ascorbic Acid 50.00
Fe(NO3)3•9H2O 0.10 d-Biotin 0.10
KCl 4,000.00 400.00 400.00 400.00 D-Ca Pantothenate 10.00 2.00 1.00 1.00
MgSO4 (anhyd.) 97.67 Choline Chloride 10.00 2.00 1.00 1.00
MgSO4•7H2O 2000.00 200.00 200.00 Folic Acid 10.00 2.00 1.00 1.00
NaCl 68,000.00 6,400.00 6,800.00 6,800.00 i-Inositol 20.00 3.60 2.00 2.00
NaHCO3 2,750.00 2,200.00 2,200.00 Nicotinamide 10.00 2.00 1.00 1.00
NaH2PO4 (anhyd.) 107.83 Pyridoxine•HCl 10.00 2.00 1.00 1.00
NaH2PO4•H2O 1400.00 124.00 140.00 140.00 Riboflavin 1.00 0.20 0.10 0.10
OTHER COMPONENTS Thiamine•HCl 10.00 2.00 1.00 1.00
Glucose 10,000.00 4,500.00 1,000.00 1,000.00 Vitamin B12 1.33
HEPES RIBONUCLEOSIDES
Lipoic Acid 0.20 Adenosine 10.00
Phenol Red•Na 100.00 15.30 10.00 10.00 Cytidine 10.00
Sodium Pyruvate 110.00 Guanosine 10.00
AMINO ACIDS Uridine 10.00
L-Alanine 25.00 Thymidine 10.00
L-Arginine•HCl 1,264.00 42.00 126.40 126.40 DEOXYRIBONUCLEOSIDES
L-Asparagine•H2O 50.00 2´Deoxyadenosine 10.00
L-Aspartic Acid 30.00 2´Deoxycytidone HCl 11.00
Cysteine•HCl•H2O 100.00 2´Deoxyguanosine 10.00
L-Cystine•2HCl 31.00 2´Deoxythymidine 10.00
L-Cystine 240.00 24.00 24.00
L-Glutamic Acid 75.00
L-Glutamine 292.00
L-Alanyl-L-Glutamine 434.00
(UltraGlutamine 1)
Glycine 50.00
L-Histidine•HCl•H2O 420.00 21.00 42.00 42.00
L-Isoleucine 524.00 52.40 52.40 52.40
L-Leucine 524.00 52.40 52.40 52.40
L-Lysine•HCl 730.00 73.10 73.00 73.00
L-Methionine 150.00 15.00 15.00 15.00
L-Phenylalanine 330.00 33.00 32.00 32.00
L-Proline 40.00
L-Serine 25.00
L-Threonine 476.00 47.60 47.60 47.60
L-Tryptophan 102.00 8.00 10.20 10.20
L-Tyrosine 362.00 36.20 36.20
L-Valine 468.00 46.80 46.80
D-Valine 46.80
Formulations for
Classical Media
316
Continued
NCTC 109 Medium Continued Richter’s CM Medium

Cat. No. 12-923 Cat. No. 12-923 Cat. No. 04-648
Liquid Liquid Liquid
COMPONENT mg/L COMPONENT mg/L COMPONENT mg/L
INORGANIC SALTS VITAMINS INORGANIC SALTS
CaCl2 (anhyd.) 200.00 p-Aminobenzoic Acid 0.125 CaCl2•2H20 264.90
KCl 400.00 Ascorbic Acid 50.00 FeCl3•6H2O 0.54
MgSO4 (anhyd.) 100.00 d-Biotin 0.025 KCl 400.00
NaCl 6,800.00 Calciferol 0.250 MgCl2•6H2O 183.00
NaHCO3 2,200.00 D-Ca Pantothenate 0.030 MgSO4•7H2O 25.00
NaH2PO4•H20 140.00 Choline Chloride 1.250 NaCl 6,800.00
OTHER COMPONENTS Folic Acid 0.025 NaHCO3 2,200.00
Cocarboxylase (TPP) 1.00 i-Inositol 0.125 NaH2PO4•H20 150.00
Coenzyme A (CoA) 2.50 Menadione 0.025 ZnSO4•7H2O 0.14
Deoxyadenosine 10.00 Niacinamide 0.063 OTHER COMPONENTS
Deoxycytidine•HCl 10.00 Niacin 0.063 Glucose 2,000.00
Deoxyguanosine 10.00 Pyridoxine•HCl 0.063 Linoleic Acid 80.00
Diphosphopyridine Nucleotide•4H2O 7.00 Pyridoxal•HCI 0.063 Lipoic Acid 0.20
Flavin Adenine Dinucleotide•2Na 1.00 DL-Tocopherol phosphate•Na2 0.025 Phenol Red•Na 10.00
D-Glucosamine•HCl 3.85 Riboflavin 0.025 Putrescine•2HCl 0.16
Glucose 1,000.00 Thiamine•HCl 0.025 Sodium Pyruvate 110.00
D-Glucuronolactone 1.80 Vitamin A Acetate 0.275 AMINO ACIDS
Glutathione (reduced) 10.00 Vitamin B12 10.00 L-Arginine•HCl 127.00
5-Methylcytosine•HCl 0.16 McQuilkin, W.T., V.J. Evans, and W.R. Earle L-Asparagine•H2O 60.00
Phenol Red•Na 20.00 (1957) J. Nat. Canc. Inst. 19:885 – 907. L-Cystine 24.00
Sodium Acetate•3H2O 50.00 L-Glutamine 584.00
Sodium Glucuronate•H2O 1.80 L-Histidine•HCl•H2O 42.00
Thymidine 10.00 L-Isoleucine 52.00
Triphosphopyridine Nucleotide•Na 1.00 L-Leucine 131.20
Tween 80 5.40 L-Lysine•HCl 72.60
Uridine 5-Triphosphate•3Na•2H2O 1.00 L-Methionine 15.00
AMINO ACIDS L-Phenylalanine 32.00
L-Alanine 31.48 L-Serine 42.00
L-Amino Butyric Acid 5.51 L-Threonine 48.00
L-Arginine•HCl 31.16 L-Tryptophan 10.00
L-Asparagine•H2O 9.19 L-Tyrosine 36.00
L-Aspartic Acid 9.91 L-Valine 46.00
L-Cysteine•HCl•H2O 288.57 VITAMINS
L-Cystine 10.49 d-Biotin 0.10
L-Glutamic Acid 8.26 Choline Chloride 56.00
L-Glutamine 135.70 D-Ca Pantothenate 1.00
Glycine 13.51 Folic Acid 2.20
L-Histidine•HCl•H2O 26.65 i-Inositol 36.00
Hydroxy L•Proline 4.09 Nicotinamide 1.00
L-Isoleucine 18.04 Pyridoxine•HCl 1.00
L-Leucine 20.44 Riboflavin 0.10
L-Lysine•HCl 38.43 Thiamine•HCl 1.00
L-Methionine 4.44 Vitamin B12 1.36
L-Ornithine•HCl 9.41
L-Phenylalanine 16.53
L-Proline 6.13
L-Serine 10.75
L-Taurine 4.18
L-Threonine 18.93
L-Tryptophan 17.50
Formulations for
Classical Media
L-Tyrosine 16.40
L-Valine 25.00
317
Continued
RPMI Medium
— Europe Only —
Cat. No. 09-774 12-115 12-167 12-918 12-702 04-525 15-702 BE12-752 BE12-115/U1 BE12-702/U1
Liquid Liquid Liquid Liquid Liquid Liquid Powder Liquid Liquid Liquid
COMPONENT mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L mg/L
INORGANIC SALTS
Ca(NO3)2•4H2O 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00
KCl 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00 400.00
MgSO4 (anhyd.) 48.83
MgSO4•7H2O 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00
NaCl 5,000.00 5,000.00 6,000.00 6,000.00 6,000.00 6,000.00 6,000.00 6,000.00 5,000.00 6,000.00
NaHCO3 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00
Na2HPO4 800.49
Na2HPO4•7H2O 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00 1,512.00
OTHER COMPONENTS
Glucose 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00 2,000.00
Glutathione (reduced) 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
HEPES 5,957.50 5,957.40 5,957.50
MOPS 34,535.00
Penicillin G Potassium (units/ml) 100
Phenol Red•Na 5.00 5.00 5.00 5.00 5.00 5.10 5.00 5.00 5.00
Streptomycin (mcg/ml) 50.00
AMINO ACIDS
L-Arginine 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00 200.00
L-Arginine•HCl 241.86
L-Asparagine•H2O 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00
L-Aspartic Acid 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Cystine•2HCl 65.19
L-Cystine 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00
L-Glutamic Acid 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Glutamine 300.00 300.00 300.00 300.00 300.00 300.00
L-Alanyl-L-Glutamine (UltraGlutamine 1) 446.00 446.00
Glycine 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00 10.00
L-Histidine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00
L-Histidine•HCl•H2O 20.27
Hydroxy L•Proline 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Isoleucine 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00
L-Leucine 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00 50.00
L-Lysine•HCl 40.00 40.00 40.00 40.00 40.00 40.00 40.00 40.00 40.00 40.00
L-Methionine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00
L-Phenylalanine 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00 15.00
L-Proline 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Serine 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00 30.00
L-Threonine 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Tryptophan 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00 5.00
L-Tyrosine 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
L-Tyrosine•2Na 28.83
L-Valine 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00 20.00
VITAMINS
p-Aminobenzoic Acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
d-Biotin 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20
D-Ca Pantothenate 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25 0.25
Choline Chloride 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00 3.00
Folic Acid 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
i-Inositol 35.00 35.00 35.00 35.00 35.00 35.00 35.00 35.00 35.00 35.00
Formulations for
Classical Media
Nicotinamide 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
Pyridoxine•HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
Riboflavin 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20 0.20
Thiamine•HCl 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00 1.00
Vitamin B12 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005 0.005
Moore, G.E., R.E. Gerner, and H.A. Franklin (1967) J. Am. Med. Assoc. 199:87 – 92.
318
Continued
Waymouth's MB 752/1 Williams' Medium E

Medium
Europe Only
Cat. No. BE12-738 Cat. No. BE12-761 BE02-019
Liquid Liquid Liquid
COMPONENT mg/L COMPONENT mg/L mg/L
INORGANIC SALTS INORGANIC SALTS
CaCl2 (anhyd.) 90.61 CaCl2 (anhyd.) 200.00 200.00
KCl 150.00 CuSO4•5H2O 0.0001 0.0001
KH2PO4 80.00 Fe(NO3)3•9H2O 0.0001 0.0001
MgCl2 (anhyd.) 112.56 KCl 400.00 400.00
MgSO4 (anhyd.) 97.67 MgSO4 (anhyd.) 400.00 400.00
NaCl 6,000.00 MgSO4 •7H2O 200.00 200.00
NaHCO3 2,240.00 MnCl2•4H2O 0.0001 0.0001
Na2HPO4 (anhyd.) 300.00 NaCl 6,800.00 6,800.00
OTHER COMPONENTS NaHCO3 2,200.00 2,200.00
Glucose 5,000.00 NaH2PO4•H2O 140.00 140.00
Glutathione (reduced) 15.00 ZnSO4•7H2O 0.0002 0.0002
Hypoxanthine Na 29.00 OTHER COMPONENTS
Phenol Red Na 10.20 Glucose 2,000.00 2,000.00
AMINO ACIDS Glutathione (reduced) 0.05 0.05
L-Arginine HCl 75.00 Linoeic Acid Methyl Ester 0.03 0.03
L-Aspartic Acid 60.00 Phenol Red Na 10.00
Cysteine HCl H2O 100.29 Sodium Pyruvate 25.00 25.00
L-Cystine 2 HCl 20.00 Tween 80 1.836 1.836
L-Glutamic Acid 150.00 AMINO ACIDS
L-Glutamine 350.00 L-Alanine 90.00 90.00
Glycine 50.00 L-Arginine 50.00 50.00
L-Histidine HCl H2O 164.10 L-Asparagine H2O 20.00 20.00
L-Isoleucine 25.00 L-Aspartic Acid 30.00 30.00
L-Leucine 50.00 L-Cysteine 40.00 40.00
L-Lysine HCl 240.00 L-Cystine 20.00 20.00
L-Methionine 50.00 L-Glutamic Acid 50.00 50.00
L-Phenylalanine 50.00 Glycine 50.00 50.00
L-Proline 50.00 L-Histidine 15.00 15.00
L-Threonine 75.00 L-Isoleucine 50.00 50.00
L-Thryptophan 40.00 L-Leucine 75.00 75.00
L-Tyrosine 2Na 2H2O 57.66 L-Lysine HCl 87.50 87.50
L-Valine 65.00 L-Methionine 15.00 15.00
VITAMINS L-Phenylalanine 25.00 25.00
Ascorbic Acid 17.50 L-Proline 30.00 30.00
d-Biotin 0.02 L-Serine 10.00 10.00
D-Ca Pantothenate 1.00 L-Threonine 40.00 40.00
Choline Chloride 250.00 L-Thryptophan 10.00 10.00
Folic Acid 0.40 L-Tyrosine 35.00 35.00
i-Inositol 1.00 L-Valine 50.00 50.00
Nicotinamide 1.00 VITAMINS
Pyridoxine HCl 1.00 Ascorbic Acid 2.00 2.00
Riboflavin 1.00 d-Biotin 0.50 0.50
Thiamine HCl 10.00 D-Ca Pantothenate 1.00 1.00
Vitamin B12 0.20 Choline Chloride 1.50 1.50
Ergocalciferol 0.10 0.10
Folic Acid 1.00 1.00
i-Inositol 2.00 2.00
Menadione Na bisulfite 0.01 0.01
Nicotinamide 1.00 1.00
Formulations for
Classical Media
Pyridoxal HCl 1.00 1.00

DL-Tocopherol phos- 0.01 0.01
phate Na2
Riboflavin 0.10 0.10
Thiamine HCl 1.00 1.00
Vitamin A Acetate 0.10 0.10
Vitamin B12 0.20 0.20
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Continued
Antibiotic Solutions
Cat. No. 09-757 17-518 17-519 17-602 17-603 17-718 17-719 17-745 17-836 02-012
17-528
COMPONENT/UNITS Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid Liquid
Amphotericin B (mg/L) 25.00 250.00
Gentamicin sulfate (mg/L) 50,000.00 10,000.00 10,000.00
L-Glutamine (mg/L) 29,200.00
NaCl (mg/L) 8,500.00 8,500.00 8,500.00 8,500.00
Potassium Penicillin 20,000.00 10,000.00 5,000.00 25,000.00 25,000.00 10,000.00
(units/ml)
Streptomycin sulfate 20,000.00 10,000.00 5,000.00 25,000.00 25,000.00 10,000.00
(mcg/ml)
Lymphocyte Separation
Balanced Salt Solutions (EBSS and HBSS) Medium
— EBSS Europe Only — — HBSS — Cat. No. 17-829
Cat. No. 10-502 02-027 04-315 10-508 10-527 10-543 10-547 Liquid
Liquid Liquid Liquid Liquid Liquid Liquid Liquid COMPONENT mg/L
COMPONENT mg/L mg/L mg/L mg/L mg/L mg/L mg/L Ficoll 400 62,000.00*
INORGANIC SALTS Sodium Diatrizoate 104,000.00
CaCl2•2H2O 265.00 265.00 186.0 186.0 Specific Gravity 1.077 g/ml
KCl 400.00 400.00 400.00 400.00 400.00 400.00 400.00 *Slight adjustments may be required
KH2PO4 60.00 60.00 60.00 60.00 60.00 to obtain the specified specific
MgSO4•7H2O 200.00 200.00 200.00 200.00 gravity
NaCl 6,800.00 6,800.00 8,000.00 8,000.00 8,000.00 8,000.00 8,000.00
NaHCO3 2,200.00 1,800.00 350.00 350.00 350.00 350.00 350.00
Na2HPO4•2H2O 158.00 158.00 MEM Non-essential
Na2HPO4•7H2O 90.00 90.00 90.00 90.00 90.00 Amino Acids
OTHER COMPONENTS
Glucose (anhyd.) 1,000.00 1,000.00 1,000.00 1,000.00 1,000.00 Cat. No. 13-114
Glucose•H2O 1,100.00 1,100.00 Liquid
HEPES 4,766.00 COMPONENT mg/L
Phenol Red•Na 10.60 10.60 20.00 20.00 L-Alanine 890.00
L-Asparagine 1,321.00
L-Aspartic Acid 1,330.00
L-Glutamic Acid 1,470.00
Glycine 750.00
Dulbecco’s Phosphate Buffered Saline (DPBS) L-Proline 1,150.00
L-Serine 1,050.00
Cat. No. 04-479 17-512 17-513 17-515
Liquid Liquid Liquid Liquid 10X
COMPONENT mg/L mg/L mg/L mg/L MEM Vitamin Solution
INORGANIC SALTS
CaCl2•2H2O 130.00 130.00 Cat. No. 13-607
KCl 200.00 200.00 200.00 2,000.00 Liquid
KH2PO4 200.00 200.00 200.00 2,000.00 COMPONENT mg/L
MgCl2•6H2O 100.00 100.00 D-Ca Pantothenate 100.00
MgCl2 (anhyd.) Choline Chloride 100.00
NaCl 8,000.00 8,000.00 8,000.00 80,000.00 Folic Acid 100.00
Na2HPO4 i-Inositol 200.00
Na2HPO4•7H2O 2,160.00 2,160.00 2,160.00 21,600.00 Nicotinamide 100.00
OTHER COMPONENTS Pyridoxine•HCl 100.00
Glucose 1,000.00 Riboflavin 10.00
Formulations for
Classical Media
Sodium Pyruvate 36.00 Thiamine•HCl 100.00
320
Cell Culture Reagent Formulations
Miscellaneous Buffer Solutions Phosphate Buffered Saline

DGV Buffer ACK Lysing HEPES TL HEPES UltraSaline A Cat. No. 04-409 17-516 17-517 BE02-017
Buffer Buffer Solution Liquid Liquid Liquid 10x Liquid
Cat. No. 10-539 10-548 17-737 04-616 12-747 Component mg/L mg/L mg/L mg/L
Liquid Liquid Liquid Liquid Liquid INORGANIC SALTS
COMPONENT mg/L mg/L mg/L mg/L mg/L CaCl2•2H2O 280.00
INORGANIC SALTS KH2PO4 144.00 144.00 1,440.00 260.00
CaCl2 (anhyd.) 20.00 NaCl 9,000.00 9,000.00 90,000.00 8,120.00
CaCl2•2H2O 300.00 Na2HPO4 795.00 795.00 7950.00
EDTA, Na2 3.72 Na2HPO4•2H2O 1,170.00
KCl 240.00 223.68 Titriplex III (EDTA Na2) 460.00
KHCO3 1001.00 LiCl 426.70
MgCl2•6H2O 100.00
MgSO4•7H2O 120.00
NaCl 8,500.00 8,500.00 6,660.00 7,130.00 Trypsin and Versene® Solutions
NaHCO3 168.00
NaH2PO4•H2O 47.60 Europe Only
Na2HPO4•7H2O 268.07 Cat. No. 17-160 17-161 17-711 BE02-007E
NH4Cl 8,023.50 Liquid Liquid Liquid Liquid
OTHER COMPONENTS Component mg/L mg/L mg/L mg/L
BSA Fraction V 3,000.00 Glucose 1,000.00 1,000.00
DL-Lactic Acid (Na Salt) 1.86 ml KCl 400.00 400.00 200.00
(60% syrup) KH2PO4 60.00 200.00
Gelatin 600.00 NaCl 8,000.00 8,000.00 8,000.00 9,000.00
Glucose 10,000.00 720.64 NaHCO3 580.00
HEPES 238,300.00 2,400.00 7,150.00 Na2HPO4•7H2O 90.00 2,170.00
Phenol Red (0.5% in 1.0 ml Phenol Red•Na 2.00
DPBS) Trypsin 25,000.00 500.00 5,000.00
Sodium Veronal 380.00 Versene® 200.00 200.00 2,000.00
Veronal 580.00 (EDTA)•2Na•2H2O
Reagents Veronal Buffer (5X)

–––– Europe Only —––– Cat. No. 12-624
ProHT™ Ultra Glutamine I Ultra Glutamine II HAT Liquid
Cat. No. BE17-855 BE17-605/U1 BE04-684 BE02-024 COMPONENT mg/L
Liquid Liquid Liquid Liquid CaCl2•2H20 185.00
COMPONENT mg/L mg/L mg/L mg/L MgCl2•6H20 840.00
INORGANIC SALTS NaCl 42,500.00
NaCl 8,500.00 8,500.00 Sodium Barbital 1,870.00
OTHER COMPONENTS Barbital 2,870.00
Aminopterin 8.80
Hypoxanthine 1,927.00 680.00
Thymidine 100.00 194.00
AMINO ACIDS
L-Arginine HCl
L-Cystine
L-Alanyl-L-Glutamine 43,444.00
(UltraGlutamine I)
L-Glycyl-L-Glutamine H2O 44,240.00
(UltraGlutamine II)
Formulations for
Classical Media
321
Notes
322
Endotoxin Detection
Chapter 6
Endotoxin Detection
Endotoxin Detection Reagents

Introduction 325
Gel Clot LAL Assays 326
Endpoint Chromogenic LAL Assay 328
Fluorescent Assay 329
LAL Accessory Products 330
Endotoxin Detection
324
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Endotoxin Detection
Fast and easy endotoxin detection for your research
Endotoxin is a pyrogen from Gram-negative bacteria. As an industry leader in endotoxin testing sales
Endotoxin impacts over 30 biological activities (including and technology, Lonza offers a wide variety of
macrophage activation, mitogenic activity, induction of assays for endotoxin detection. For the research
interferon and colony stimulating factor). Endotoxin can customer, we have included in this catalog Gel Clot
lead to cell death by intitiating complement activation. LAL (PYROGENT®), Endpoint Chromogenic LAL
(QCL-1000®), and PyroGene® Recombinant Factor
Endotoxin Detection
Blood cells (amebocytes) from horseshoe crabs were C Assays, as well as instrumentation, software and
found to clot in the presence of endotoxin and this accessories to run these assays.
technology was used in the development of endotoxin
detection assays. The Limulus Amebocyte Lysate (LAL) In addition, Lonza offers kinetic quantitative assays and a
test was commercially introduced in the 1970’s. More Testing Service. More information can be found on the web
recently, Lonza scientists have produced a recombinant at www.lonza.com or call Scientific Support.
form of Factor C, the first component in the Limulus
polyphemus clotting cascade activated by endotoxin.
325
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Gel Clot LAL Assays
PYROGENT® Gel Clot LAL Assay Ordering Information

The PYROGENT® Gel Clot LAL Assay is a qualitative LAL
Cat. No. Size Sensitivity EU/ml Price
test for Gram-negative bacterial endotoxin. PYROGENT®
Assays are available as a lysate kit with sensitivities PYROGENT® Gel Clot LAL
Contains:
of 0.03, 0.06, 0.125 and 0.25 EU/ml. Standard kit sizes 80 tests = 5 ✕ 16 tests/vial of lysate
include 250 tests, 80 tests, and a 25 test-kit in a single 250 tests = 5 ✕ 50 tests/vial of lysate
test vial format. These kits do not include a matched N104 250 tests 0.03
control standard endotoxin. However, the standard can be N183-06 80 tests 0.06
purchased separately (see Control Standard Endotoxin, page N194-06 250 tests 0.06
327). For your convenience, Certificates of Analysis are N183-125 80 tests 0.125
available at www.lonza.com/coa. N184-125 250 tests 0.125
■ Storage Conditions N184-25 250 tests 0.25
2°C – 8°C PYROGENT® Gel Clot LAL Single Test Vials

Contains: 25 single test vials of lysate
N189-06 25 tests 0.06

N189-125 25 tests 0.125
N189-25 25 tests 0.25
Gel Clot LAL Assays
PYROGENT® Plus Gel Clot LAL Assay Ordering Information

The PYROGENT® Plus Gel Clot LAL Assay combines
PYROGENT® LAL with a matched control standard Cat. No. Size Sensitivity EU/ml Price
endotoxin. For your convenience, the Certificate of PYROGENT® Plus Gel Clot LAL Assay
Analysis documenting the FDA and USP required RSE/ Contains:
1 ✕ 10 ng/vial endotoxin
CSE correlation is available at www.lonza.com/coa. 64 Tests = 4 ✕ 16 tests/vial of lysate
PYROGENT® Plus Kits are available with sensitivities of 200 Tests = 4 ✕ 50 tests/vial of lysate
0.03, 0.06, 0.125 and 0.25 EU/ml. Standard kit sizes are N294-03 200 tests 0.03
200 tests, 64 tests, and a 24-test kit in a single test vial N283-06 64 tests 0.06
format is available. N294-06 200 tests 0.06
N294-125 200 tests 0.125
2°C – 8°C
N284-25 200 tests 0.25
PYROGENT® Plus Gel Clot LAL Single Test Vials

Contains:
1 ✕ 1 ml vial endotoxin
24 single test vials of lysate
N289-06 24 tests 0.06

N289-125 24 tests 0.125
N289-25 24 tests 0.25
326
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Gel Clot LAL Assays
PYROGENT® Ultra Gel Clot LAL Assay Ordering Information

The PYROGENT® Ultra Gel Clot LAL Assay contain 4 vials
of lysate and a series of pre-mixed liquid endotoxin
standards that are at the concentrations necessary for a PYROGENT® Ultra Gel Clot LAL Assay
Contains:
standard curve and positive product control. This results in 200 Tests = 4 ✕ 50 tests/vial of lysate
substantially reduced preparation time. Standard dilutions 5 ✕ 5 ml liquid endotoxin standards
are 2 lambda, 1 lambda, 0.5 lambda, 0.25 lambda, and 20 N594-03 200 tests 0.03
lambda. Each kit is sufficient for 200 tests. N594-06 200 tests 0.06
2°C – 8°C
Gel Clot LAL Assays

Control Standard Endotoxin for Gel Clot LAL
Lonza’s Control Standard Endotoxin is referenced against Ordering Information

the USP Reference Standard Endotoxin following the
procedures set forth in the “Guidelines on Validation of Cat. No. Size Sensitivity EU/ml Price
the Limulus Amebocyte Lysate Test as an End-Product Endotoxin (E. coli) for Gel Clot Assays
Endotoxin Test for Human and Animal Parenteral Drugs, Strain O55:B5
Biological Products, and Medical Devices”. Certificates of N185 5 vials ✕ 2.5 mg/vial*
Analysis showing potency are available upon request. N186 5 vials ✕ 10 ng/vial
2°C – 8°C
* high potency for depyrogenation studies

327
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Endpoint Chromogenic LAL Assay
QCL-1000® Endpoint Chromogenic LAL Assay Ordering Information

The QCL-1000® EndPoint Chromogenic LAL Assay, the
most rapid of the LAL tests, is a 16-minute, quantitative
endpoint assay that has a sensitivity range from 1.0 EU/ QCL-1000® Endpoint Chromogenic LAL Assay
ml to 0.1 EU/ml. This chromogenic LAL method forms a 50-647U 120 tests 0.1 – 1.0
yellow color and is measured photometrically at 405
Contains:
– 410 nm. With the QCL-1000® Assay, a multichannel 5 ✕ 1.4 ml vials lysate
pipette and a 96-well plate, you can run 96 reaction wells 1 ✕ 1 ml vial endotoxin
2 ✕ 30 ml vials LAL Reagent Water
at one time. This assay can also be run in tubes. For your 2 ✕ 6.5 ml vials chromogenic substrate
convenience, Certificates of Analysis are available at 50-648U 300 tests 0.1 – 1.0
www.lonza.com/coa. Contains:
5 ✕ 3 ml vials lysate
■ Storage Conditions 2 ✕ 1 ml vial endotoxin
5 ✕ 6.5 ml vials chromogenic substrate
2°C – 8°C
Endotoxin for QCL-1000® Assay
Strain O111:B4
E50-640 1 ✕ 1 ml vial endotoxin

Approximately 15 – 40 EU/ml
Endpoint Chromogenic
LAL Assay
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Fluorescent Assay
PyroGene®Recombinant Factor C Assay Ordering Information

The PyroGene® Recombinant Factor C Assay offers
improvements for endotoxin detection testing. Lonza
scientists have produced a recombinant form of Factor C, PyroGene® Recombinant Factor C Assay
the first component in the Limulus polyphemus clotting 50-658U 192 Tests 0.01 – 10
cascade activated by endotoxin. Recombinant Factor C Contains:
(rFC) is activated by endotoxin binding, and the active 2 ✕ 1.2 ml vials rFC
moiety created then acts to cleave a synthetic substrate 2 ✕ 6 ml vials fluorogenic substrate
2 ✕ 5 ml vials assay buffer
which results in the generation of a fluorogenic compound. 2 ✕ 10 ng /vial endotoxin
The reaction is run in a 96-well microplate and measured at 2 ✕ 30 ml vials LAL Reagent Water
time zero and after a one-hour incubation in a fluorescent For instrumentation and software see page 324
microplate reader using excitation/emission wavelengths
of 380/440 nm.
Advantages of PyroGene® Assay:
— Greater endotoxin specificity The rFC Assay and LAL Assays measure similar endotoxin
— Elimination of false positive glucan reactions potency
Relative Endotoxin Potency
— Less lot-to-lot variability Kinetic-QCL® Assay
2.0 PyroGene® Assay
— No animal utilization PYROGENT®-5000 Assay
Fluorescent Assay
The PyroGene® Assay represents a reliable, 1.5
environmentally friendly alternative to LAL. We invite you

to experience the PyroGene®Assay alternative. 1.0
2°C – 8°C 0.5
0.0 EC-6 O55:B5 Pseudomonas
Similar endotoxin potency is detected in purified lipopolysaccharides using

PyroGene®, Kinetic-QCL® (kinetic chromogenic) and PYROGENT®-5000 (kinetic
turbimetric) Assays.
rFC endotoxin standard curves using 4 different lots of rFC

Fluorescence (FU)
1e+5
Lot 061101
Lot 051401
1e+4 Lot 111201
Lot 041601
1e+3
1e+2
1e+1
1e+0 0.001 0.01 0.1 1 10 100

EC-6 Endotoxin (EU/ml)
The log net fluorescence is proportional to the log endotoxin concentration

and is linear in the 0.01 – 10 EU/ml range. Lot-to-lot standard curves exhibit
excellent reproducibility.
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LAL Accessory Products
Name Cat. No. Size Storage Conditions Comments Price
Reagents
b-G-Blocker N190 5 ✕ 5 ml vials 2°C – 8°C Used to block glucan interference
PYROSPERSE® Dispensing Agent N188 5 ✕ 5 ml vials 2°C – 30°C Dispersing agent
MgCl2 S50-641 30 ml vial 2°C – 30°C 10 mM Solution
Tris Buffer S50-642 30 ml vial 2°C – 30°C 50 mM Solution
LAL Reagent Water W50-640 30 ml 2°C – 8°C <0.005 EU/ml
W50-100 100 ml 15°C – 30°C <0.005 EU/ml
W50-500 500 ml 15°C – 30°C <0.005 EU/ml
Name Cat. No. Size Quantity Comments Price
Labware
Pyrogen-free Test Tubes N201 10 ✕ 75 mm 50/pkg w/polypropylene screw caps
Pyrogen-free Test Tubes N205 10 ✕ 75 mm 50/pkg Foil wrapped without caps
LAL Accessory Products
Pyrogen-free Dilution Tubes N207 13 ✕ 100 mm 30/pkg Foil wrapped without caps
Reagent Reservoir 25-364 — 10/pkg For use with 8-channel pipettes
Sample Container, 80-507L 10 ml 25/box Glass bottles with screw caps
Depyrogenated 80-507U 100/box
Eppendorf® Biopur 25-415 200 µl 5 trays/pkg;
Pipette Tips 96 tips/tray
25-416 300 µl Only for multichannel pipettors
25-417 1,000 µl
Stepped Neutral 25-342 — For use in ELx808LBS plate readers
Density Plate
Ground Aluminum 25-038 — Each Holds a 96-well plate in a dry bath
Block with Lid multiblock heater
LAL Reagent Grade Plates 25-340 — 50/case 96-well, <0.0005 EU/well
Name Cat. No. Comments Price
Instrumentation and Software

ELx808LBS Incubating Microplate Reader 25-315 For use with chromogenic assays
FLx800LBS Fluorescent Microplate Reader with Ex/Em 380/440 25-344 For use with fluorogenic assay
WinKQCL® 4 Software Package 00189910 For use with ELx808LBS and FLx800LBS plate readers
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Flexible Bioprocess Containers
Chapter 7

Introduction 333
Platinum UltraPAK™ Bioprocess Containers 336
Bioprocess Tank Liners 337
ProEVA PAK™ Bioprocess Containers 338
Flexible Bioprocess
Containers
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Lonza is committed to providing innovative products durability and flexibility. Aside from having superior
for the life science community. Our Platinum UltraPAK™ gas barrier properties, undetectable endotoxin, and
Bioprocess Containers are no exception, providing the certification as a USP Class VI material, our film is
best single-use container for your bioprocessing needs. non-animal in origin (NAO). As a result, our film provides
complete protection and reliability to many products that
Flexible bioprocess containers are the efficient alternative come in contact with it such as:
Flexible Bioprocess
packaging to traditional, rigid wall containers used for the
■ Cell culture media
transfer of liquid media, buffers, or product intermediates.
Containers
Platinum UltraPAK™ Single-use, Pillow-style Containers ■ Buffers
are available in multiple configurations using a 4-web ■ Water for Injection (WFI)
proprietary film. For the past 10 years, Lonza has
■ Final product yielded from downstream manufacturing
continually produced high quality, flexible packaging
for the pharmaceutical and biotech community. Lonza processes
bioprocess containers offer extensive customization We chose a four-web, polyethylene film design, providing
capabilities with a large variety of connectors and tubing more flexibility and less rigidity compared to single-web
types, while maintaining a short turn around time for films, without sacrificing durability. More rigid films have
custom made bags. a tendency to generate pinhole leaks.
Lonza Platinum UltraPAK™ Bioprocess Containers are Quality Systems

available in multiple sizes from 50 ml up to 3000 L, in Platinum UltraPAK™ Bioprocess Containers are
pillow-style or 3D, made from the same film material, manufactured under strict quality standards to ensure
eliminating the time consuming task of validating the integrity of the product. All of our packaging is
another film when scaling up production. As with all of our manufactured in an FDA registered, class 10,000 clean
products, quality is incorporated into every aspect of our room, adhering to cGMP and ISO 9001 standards. Film,
manufacturing process, from raw materials to the finished fitment and perimeter seals undergo thorough visual
product. inspection. Furthermore, we routinely perform leak
testing according to ASTM F2095-01. From 50 ml to
Platinum UltraPAK™ Film 3000 L, our flexible bioprocess containers can be
An important component of our packaging is our customized to match your specific application.
proprietary polyethylene film formulation. The Platinum
UltraPAK™ Film was created by our engineers to provide
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Validation Testing Methods & Results

The list below describes the validation testing methods Platinum UltraPAK™ Film were filled with Trypticase Soy
that were performed on the inner and outer layers of the Broth. The filled systems were incubated at two separate
Platinum UltraPAK™ Polyethylene Film, followed by actual temperatures: (1) at 20˚C – 25˚C for two weeks followed
testing results. by; (2) 30˚C – 35˚C for an additional two weeks. After
incubation, the filled bioprocess containers were shipped
Validation Test Method cross-country, returning to the point of origin and
Endotoxin – Testing was completed by the Quantitative incubated a second time at the two separate temperature
Chromogenic LAL method on irradiated (25-45 kGy) ranges and durations as described previously. Integrity
film. Endotoxin was not detected in the manufacturing was maintained during this stringent test, with no
process. evidence of contamination.
Physiochemical Assay – The physical and chemical Brittleness Temperature Test – Samples of irradiated
properties of the film and its extracts, using the and artificially aged irradiated film were subjected to
Non-volatile Residue, Residue on Ignition, Heavy Metals –80˚C in accordance with ASTM D1790-02. The Platinum
and Buffering Capacity Assays, were determined UltraPAK™ Film showed no visible evidence of damage
according to the protocol referenced from USP <661> when subjected to this rigorous test.
Containers Physiochemical Tests-Plastics.
Freezing Integrity Study – To ensure the durability of
Flexible Bioprocess
In vitro and In vivo Biological Reactivity Tests – Samples our flexible packaging as a complete system for frozen
of irradiated and artificially aged irradiated film were applications and conditions, bioprocess containers
Containers
assayed for biological reactivity with mammalian cell produced with irradiated Platinum UltraPAK™ Film were
culture and biological response of animals following the filled with Trypticase Soy Broth. The filled systems were
requirements of Class VI plastics, as specified in USP incubated at two separate temperatures: (1) at 20° C - 25°C
<87> Biological Reactivity Test, in vitro and in vivo. for two weeks followed by; (2) 30°C - 35°C for an additional
two weeks, to demonstrate sterility prior to freezing.
Moisture Vapor Transmission Rate – We demonstrated After incubation, the filled bioprocess containers were
the barrier properties of irradiated and artificially aged frozen to – 20°C and incubated a second time at the two
irradiated film by determining the Rate of Water Vapor separate temperature ranges and durations as described
Transmission (in grams/100 square inches/day). The previously. Integrity and sterility were maintained during
quantitative data was determined by performing the this rigorous test with no evidence of contamination.
protocol referenced from ASTM F1249-01.
Polyethylene with Additives for Containers for Parenteral
Oxygen and Carbon Dioxide Transmission Rate – We Preparation EP 5.4, 2006, 3.1.5 – Irradiated bags were
demonstrated the barrier properties of irradiated and tested to meet the requirements of the European
artificially aged irradiated film by determining the Rate of Pharmacopoeia regarding “polyethylene with additives for
Oxygen and Carbon Dioxide Transmission (in grams/100 containers for parenteral preparation and for ophthalmic
square inches/day). The quantitative data was determined preparations”. The study was based upon the European
by performing the protocol referenced from ASTM D3985. Pharmacopoeia current version: Chapter 3.1.5. Based on
the evaluation criteria, Platinum UltraPAK™ Bioprocess
Heat Seal and Fitment Seal Strength – To ensure the Containers meet the requirements of the EP current
strength of a heat sealed seam (either the film to itself version, Chapter 3.1.5.
or the film to a port), we performed an assay referenced
from ASTM D882. To test the heat seal strength, all four Plastic Containers for Parenteral Infusion EP 5.4, 2006,
sides of a pillow-style flexible container were assayed. 3.2.2.1 – Irradiated bags were tested to meet the
The data demonstrated superior strength. requirements of the European Pharmacopoeia regarding
plastic containers for aqueous solutions for parenteral
Integrity Testing – To ensure the durability of our flexible infusion. Based on the evaluation criteria, Platinum
packaging as a complete system, bioprocess containers UltraPAK™ Bioprocess Containers meet the requirements
produced with irradiated and artificially aged irradiated of the EP 5.4, 2006, Chapter 3.2.2.1.
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Validation Test Results
Assay Irradiated Film Artificially Aged Irradiated Film

Endotoxin Less than 0.005 EU/ml N/A
Class VI Plastic Meets USP Class VI Meets USP Class VI
Physiochemical Testing Meets <661> per USP N/A
– Non-Volatile Residue
– Residue on Ignition
– Heavy Metals
– Buffering Capacity
In-Vitro Biological Reactivity Meets <87> per USP Meets <87> per USP
ISO Elution Method No evidence of cell lysis or toxicity No evidence of cell lysis or toxicity
In-Vivo Biological Reactivity Meets <88> per USP Meets <88> per USP 25
– Systemic Toxicity No mortality or evidence of systemic toxicity; No mortality or evidence of systemic toxicity;
– Intracutaneous Toxicity no evidence of irritation and insignificant no evidence of irritation and insignificant
– Muscle Implantation macroscopic reaction macroscopic reaction
Moisture Vapor Transmission Rate 0.09 gm/100in2/day 0.09 gm/100in2/day
Oxygen Transmission Rate 0.103 cc/100in /day 2 0.092 cc/100in2/day
Carbon Dioxide Transmission Rate 0.27 cc/100in2/day 0.24 cc/100in2/day
Heat Seal Strength No failure during testing No failure during testing
– Bottom Seal 31.09 lb/in 32.24 lb/in
– Top Seal 30.44 lb/in 33.20 lb/in
– Right Seal 27.27 lb/in 29.09 lb/in
Flexible Bioprocess
– Left Seal 27.57 lb/in 29.76 lb/in
Fitment Seal Strength
Containers
– Width 41.7 lb/in 26.4 lb/in
– Length 28.3 lb/in 39.0 lb/in
– Perpendicular 102.4 lb/in 105.0 lb/in
Shipping Integrity Test Sterile Sterile
Brittleness Temperature Test -80° C, no evidence of visible damage -80° C, no evidence of visible damage
Freezing Integrity Test Integrity of the bag is kept; no visual signs of N/A
contamination of the medium after incubation
Polyethylene with additives for Meets EP 3.1.5 N/A
containers for parenteral preparation
and for ophthalmic preparations
Plastic containers for aqueous solutions Meets EP 3.2.2.1 N/A
for parenteral infusion
The Platinum UltraPAK™ Outer Film material is a laminate of nylon and

polyethylene, providing structural support and excellent gas barrier properties.
Inner film layer Polyethylene

construction
{
Polyetheylene
EVOH
Outer film layer Polyethylene
construction Tie layer
PVDC
Nylon
The Platinum UltraPAK™ Film fluid contact layer is a high quality polyethylene.
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Platinum UltraPAK™ Advantages Tubing Sets and Connectors

Aside from the superior properties of our film, Lonza Lonza can design and produce a myriad of different tubing
engineers continue to research new designs and features sets to meet your specific applications. With multiple
to enhance the benefits of our product. Platinum UltraPAK™ tubing types and connectors at our disposal, designing
Bioprocess Containers offer features such as: a specific configuration unique to your applications is
— Continuous sealing process – 50 ml – 200 L bags easy for us to produce. When used in conjunction with
have a continuous seal generated by our automated our Platinum UltraPAK™ Bioprocess Containers, you can
sealing process, eliminating possible seam leaks be confident in solving connectivity issues efficiently,
every time.
— Unmatched versatility in connectivity – Standard
systems offer MPC and luer connections, along with Standard Platinum UltraPAK™ Bag Configurations
needle-less injection sites for sampling; custom The list below describes the components on our premium
systems offer additional connectivity devices such as pre-made containers. All bags are individually wrapped
steam-in-place (SIP) and aseptic connections, just to and irradiated at 25-45 kGy.
name a few
— Convenient carrier handles – Available on 5 L, 10 L and
20 L sizes; the carrier handle is sealed separately and Cat. No. Size Price
does not contact the inner product, eliminating areas Platinum UltraPAK™ w/C-Flex® Tubing 1 L
of gas transmission Port 1: 18" of 1/4" C-Flex® tubing with female luer needle-less injection site
Flexible Bioprocess
Port 2: 18" of 1/4" PVC tubing with male MPC with female cap
— Robust ports – Lonza's goal was to maximize the
08-E1322 1 L, 25 bags per case
Containers
surface area of the Platinum UltraPAK™ Film contacting

the inner product; as a result, our ports offer a small Platinum UltraPAK™ w/C-Flex® Tubing 5 L, 10 L, 20 L
surface area, minimizing a point of gas exchange, and Port 1: 18" of 1/4" C-Flex® tubing with female luer needle-less injection site
at the same time, maintaining a robust connection Port 2: 18" of 1/4" silicone tubing with female MPC with male cap
Port 3: 18" of 1/4" silicone tubing with male MPC with female cap
between the barb and tubing Hanging Bar
Platinum UltraPAK™ w/C-Flex® Tubing 50 L, 100 L, 200 L

Port 1: 18" of 1/4" C-Flex® tubing with female luer needle-less injection site
Port 2: 72" of 1/4" silicone tubing with male MPC with female cap
Port 3: 72" of 1/4" silicone tubing with female MPC with male cap
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Bioprocess Tank Liners Ordering Information – Tank Liners

Bioprocess tank liners are the efficient, alternative Cat. No. Size Price
solution to traditional, rigid wall containers used for the
Pillow-Style Tank liners
transfer of liquid media, buffers, or product intermediates.
Single-use, pillow-style tank liners are available in sizes 08-E269 50 L, 20 bags per case 13” x 27”
ranging from 50 L to 1360 L, and gusseted tank liners 08-E270 130 L, 20 bags per 18" ✕ 33"
case
are available in 50 L to 200 L using a LLDPE film.
08-E271 200 L, 20 bags per 22.5" ✕ 34".
— Validated – Passes USP Class VI Medical Grade Testing case
08-E272 340 L, 20 bags per 24" ✕ 48"
— Polyethylene – Product contact layer and a poly- case
ethylene/nylon barrier layer that offers durability and 08-E273 400 L, 20 bags per 28" ✕ 42"
superior gas barrier properties case
— Non-Animal Origin – The film is completely non-animal 08-E275 560 L, 10 bags per 30" ✕ 48"
case
origin (NAO), including slip agents used in the 08-E276 790 L, 10 bags per 38" ✕ 48"
manufacturing process case
— Integrity Tested – Complete systems were rigorously 08-E277 1,090 L, 10 bags per 42" ✕ 48"
case
tested to ensure shipping stability and durability
08-E278 1,360 L, 10 bags per 48" ✕ 48"
— Brittleness Temperature Tested – Subjected to -80°C case
Flexible Bioprocess
in accordance with ASTM D1790-02
Gusseted-Style Tank liners
— Broad Range of Sizes – Pillow-style tank liners range
Containers
08-E282 50 L, 20 bags per case 13.5” x 29”
in size from 50 L to 1360 L, while our gusseted tank
08-E283 100 L, 20 bags per 18.5" ✕ 33"
liners range in size from 50 L to 200 L case
— Convenient Single-Use – Eliminates cross-contami- 08-E284 200 L, 20 bags per 22.5" ✕ 38"
case
nation; cleaning of dedicated equipment and cleaning
validation
— Gamma Irradiated – Off-the-shelf ready; no in-plant
sterilization needed
— Flexibility – Tank liners provide quick scale-up and
scale-down; reduces loss time between manufacturing
campaigns
— Minimal Training – Nominal operator training and
preparation time
All bioprocess tank liners are manufacturing in a cGMP,
FDA Registered, class 10,000 clean room to ensure
product consistency and quality assurance. Quality
testing includes complete cGMP traceability and exceeds
ISO 9001 standards.
The LLDPE film is tested for thickness, seal properties,
and bond strength prior to manufacturing. A thorough
visual inspection is conducted on tank liner film after
manufacturing and prior to irradiation.
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ProEVA PAK™ Bioprocess Containers Standard ProEVA PAK™ Bag Configurations

Flexible bioprocess containers are the efficient, alternative The list below describes the components on our premium
packaging to traditional, rigid wall containers used for the premade containers. All bags are individually wrapped and
transfer of liquid media, buffers, or product intermediates. irradiated at 25-45 kGy.
Our ProEVA PAK™ Single-use, Pillow-style Bioprocess Ordering Information
Containers are available in 1, 5, 10 and 20 L standard
configurations using a 4-web LDPE based on EVA film. Cat. No. Size Price
ProEVA PAK™ 1 L
Lonza ProEVA PAK™ Bioprocess Containers have the Port 1: male luer with 3/16" barb
Port 2: female luer with 3/16" barb
following features: Port 3: stays the same break-off injection site
— Ethylene-Vinyl Acetate – Product contact layer and a 08-E287 1 L, 25 units per case
polyethylene/EVOH barrier layer that offers durability
ProEVA PAK™ 5 L, 10 L, and 20 L
and superior gas barrier properties
Port 1: male MPC with 1/4" barb
— Unmatched Versatility in Connectivity – Our standard Port 2: male MPC with 1/4" barb
Port 3: female luer with 3/16" barb with male luer cap and built in injection
systems offer MPC and luer connections, along with site
needleless injection sites for sampling 08-E279 5 Liters, 20 units per case
— Continuous Sealing Process – Generated by our auto- 08-E280 10 Liters, 20 units per case
Flexible Bioprocess
mated sealing process, eliminating possible seam 08-E281 20 Liters, 20 units per case
leaks
Containers
— Convenient Carrier Handles – Available on our 5, 10

and 20 L sizes; the carrier handle is sealed separately
and does not contact the inner product eliminating
areas of gas transmission
— Robust Ports – Offering small surface area, minimizing
a point of gas exchange, while maintaining a robust
connection between the barb and tubing
— Validated – Passes USP Class VI Medical Grade Testing
— Non-Animal Origin – Both layers are completely
non-animal origin (NAO) including slip agents used in
the manufacturing process
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Custom Services
Chapter 8
Lonza Custom Manufacturing
Lonza established the concept of custom manufacturing A Snapshot of Lonza

over 25 years ago, recognizing the trend toward
— Dedicated custom manufacturing organization (CMO)
outsourcing in the pharmaceutical industry.
— Proven track record (over 25 years)
Since then, Lonza Custom Manufacturing has been — Focused on the life-sciences market
helping both emerging and established pharmaceutical
— Worldwide, multi-product cGMP facilities
and Biopharmaceutical companies improve and advance
their products. — Pre-clinical to commercial supply
— Full R&D service provider
Whether for pre-clinical or commercial supply, Lonza’s
broad technology platforms, complete range of Broad Product Experience
development services, and cutting-edge manufacturing — APIs & HAPIs
processes enable your products to reach their full potential
within a desirable timeframe. — Advanced intermediates
— Monoclonal antibodies
— Antibody fragments
Custom Services
— Recombinant proteins
— Cell-based therapeutics
— Drug conjugates
— Vaccines
— Plasmid DNA
— Peptides
— Oligonucleotides
— Fine chemicals
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Lonza Custom Manufacturing
Five Key Technologies Complete R&D Services

— Advanced Chemical Synthesis — AggreSolve™ Protein Aggregation Predictor
— Peptide & Oligonucleotide Synthesis — The GS Gene Expression System™
— Microbial Fermentation — XS Microbial Expression Technologies™
— Mammalian Cell Culture — Cell line/Strain design
— Cell Therapy — Bio/Catalyst design
— Process development
— Process scale-up
— Process validation
— Process transfer
— Technology development
— Regulatory support
Contact LCM Sales & Marketing
Slough Verviers
Braine Kourim
Conshohocken Visp
Portsmouth
Porriño
Walkersville Hopkinton
Nansha
Custom Services
Singapore
North America Rest of World GS System™

Steve Johnson Olivier Loeillot E-mail: GSLonza@lonza.com
Lonza, Inc. Lonza, Ltd Tel: +44 1753 777 000
90 Boroline Road Muenchensteinerstrasse 38 www.lonza.com/gene
Allendale. NJ 07401 CH-4002 Basel expressions.html
United States Switzerland
Tel: +1 201 316 9200 Tel: +41 61 316 81 11
E-mail: contact.us@lonza.com E-mail: contact@lonza.com
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Transfection Services
■ Protocol optimization for either your Nucleofector® II For more information about protocol optimization or
Device or your 96-well Shuttle® System development of stably transfected cells, please contact
■ Generation of fully characterized stable transfectants your local Sales Representative.
All of Lonza services are designed to meet your needs and

to assist you in successfully achieving your transfection
goals. The Lonza Services Team has a broad range of
expertise to offer you the most efficient solution to your
research needs while guiding you through the entire
process quickly.
Meet key milestones and objectives in your

research faster
Lonza Scientists will optimize transfection protocols using
our proprietary non-viral technology for virtually any
cell type and substrate, allowing you the freedom to focus
your expertise on analysis and experimentation, not the
creation of the clone or protocol.
A service you can trust

Lonza’s team of scientists has years of transfection
experience. They will provide you with fast, reliable results
along with expert scientific support.
■ Lonza's in-house scientists will:

– Optimize transfection parameters for either the
Nucleofector® II Device or 96-well Shuttle® System for
your cell lines and primary cells
Custom Services
– Create stably transfected cells in either pools of stable

transfectants or single cell-derived clones
■ After optimizing the transfection conditions
you will receive:
– Detailed description of the Nucleofection® Procedure
– Nucleofection® Parameters for optimal results
– Data on transfection efficiency and viability
– A detailed description of cell handling before and
after Nucleofection®
■ After generation of stable transfectants
you will receive:
– Your stably transfected cells (batch or single clones)
– Detailed information on how clones were generated
– Clone data sheet including expression
characterization
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Indices
Chapter 9
Numerical Index
Cat. No. Description Page Number Cat. No. Description Page Number
50568 Transport™ Reagent 400 µg . . . . . . . . . . . . . . . . . . . . . . 202 00188235 Huntington’s Disease 2 x 96-well . . . . . . . . . . . . . . . . . .212
77201 HL-1™ Liquid w/o L-Glut 2 × 500 ml . . . . . . . . . . . . . . . . 274 00188236 Immune Complement 2 x 96-well . . . . . . . . . . . . . . . . .212
77204 HL-1™ Powder Kit w/o L-Glut, 50 L . . . . . . . . . . . . . . . . . 274 00188237 Immune System Phenotyping 2 x 96-well . . . . . . . . . .212
77227 HL-1™ Supplement 100X 10 ml . . . . . . . . . . . . . . . . . . . . 274 00188238 Immunological Targets of NFKB 2 x 96-well . . . . . . . . .212
77232 PC-1™ Liquid 2 × 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 263 00188239 Innate Immune Signaling 2 x 96-well . . . . . . . . . . . . . .212
00188072 Alzheimer’s Disease 2 x 96-well . . . . . . . . . . . . . . . . . .214 00188240 Interferon Signaling & Response 2 x 96-well . . . . . . .212
00188073 Antigen Processing & Presentation 2 x 96-well . . . . .214 00188241 Mood Disorder 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . .212
00188074 Apoptosis 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .214 00188242 Neurodegeneration 2 x 96-well . . . . . . . . . . . . . . . . . . . .213
00188075 Autophagy 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .214 00188243 NFKB Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . .213
00188076 B cell Developmental and Function 2 x 96-well . . . . .214 00188244 Notch Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . .213
00188077 B cell Receptor and Signaling 2 x 96-well . . . . . . . . . . .214 00188245 Obesity 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .213
00188078 Blood Coagulation 2 x 96-well . . . . . . . . . . . . . . . . . . . . .214 00188246 Osteoporosis 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . .213
00188079 Cell Cycle Tox and Cancer 2 x 96-well . . . . . . . . . . . . . .214 00188247 p53 Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . .213
00188139 Cytokines and Receptors 2 x 96-well . . . . . . . . . . . . . .214 00188248 Parkinson’s 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . .213
00188140 DNA Damage and Repair 2 x 96-well . . . . . . . . . . . . . . .214 00188249 Stress Response 2 x 96-well . . . . . . . . . . . . . . . . . . . . . .213
00188161 Electron Transport Toxicology 2 x 96-well . . . . . . . . . .214 00188250 T cell Receptor Signaling 2 x 96-well . . . . . . . . . . . . . . .213
00188162 FoxP3 Target Genes 2 x 96-well . . . . . . . . . . . . . . . . . . .214 00188251 T helper 17 (Th17) 2 x 96-well . . . . . . . . . . . . . . . . . . . . .213
00188163 General Toxicology 2 x 96-well . . . . . . . . . . . . . . . . . . . .214 00188252 T Regulatory Phenotyping 2 x 96-well . . . . . . . . . . . . .213
00188164 Growth and Development Tox 2 x 96-well . . . . . . . . . . .215 00188253 Targets of Wnt/ß-catenin Signaling 2 x 96-well . . . . .213
00188165 Hematopoetic Cell Lineage 2 x 96-well . . . . . . . . . . . . .215 00188254 TGF-ß Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . .213
00188166 Huntington’s Disease 2 x 96-well . . . . . . . . . . . . . . . . . .215 00188255 Toll-like Receptor Signaling 2 x 96-well . . . . . . . . . . . . .213
00188167 Immune Complement 2 x 96-well . . . . . . . . . . . . . . . . .215 00188256 Wnt Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . .213
00188168 Immune System Phenotyping 2 x 96-well . . . . . . . . . .215 00188257 Alzheimer’s Disease 2 x Fast 96-well . . . . . . . . . . . . . 211
00188169 Immunological Targets of NFKB 2 x 96-well . . . . . . . . .215 00188258 Antigen 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . . . 211
00188170 Innate Immune Signaling 2 x 96-well . . . . . . . . . . . . . .215 00188259 Apoptosis 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . 211
00188171 Interferon Signaling & Response 2 x 96-well . . . . . . .215 00188260 Autophagy 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . 211
00188172 Mood Disorder 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . .215 00188261 B cell Receptor and Signaling 2 x Fast 96-well . . . . . 211
00188173 Natural Killer Cell 2 x 96-well . . . . . . . . . . . . . . . . . . . . . .215 00188262 Blood Coagulation 2 x Fast 96-well . . . . . . . . . . . . . . . 211
00188174 Neurodegeneration 2 x 96-well . . . . . . . . . . . . . . . . . . . .215 00188263 Cell Cycle Tox and Cancer 2 x Fast 96-well . . . . . . . . . 211
00188183 NFKB Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . .215 00188264 Cytokines and Receptors 2 x Fast 96-well . . . . . . . . . 211
00188184 Notch Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . .215 00188265 DNA Damage and Repair 2 x Fast 96-well . . . . . . . . . .212
00188185 Obesity 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .215 00188266 Electron Transport Toxicology 2 x Fast 96-well . . . . . .212
00188186 Osteoporosis 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . 216 00188267 FoxP3 Target Genes 2 x Fast 96-well . . . . . . . . . . . . . . .212
00188187 p53 Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . 216 00188268 General Toxicology 2 x Fast 96-well . . . . . . . . . . . . . . . .212
00188188 Parkinson’s 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . 216 00188269 Growth and Development Tox 2 x Fast 96-well . . . . . .212
00188189 Pathogen Recognition 2 x 96-well . . . . . . . . . . . . . . . . 216 00188270 Hematopoetic Cell Lineage 2 x Fast 96-well . . . . . . . .212
00188190 Stress Response 2 x 96-well . . . . . . . . . . . . . . . . . . . . . 216 00188271 Huntington’s Disease 2 x Fast 96-well . . . . . . . . . . . . .212
00188191 T cell Receptor Signaling 2 x 96-well . . . . . . . . . . . . . . 216 00188272 Immune Complement 2 x Fast 96-well . . . . . . . . . . . . .212
00188192 T helper 17 (Th17) 2 x 96-well . . . . . . . . . . . . . . . . . . . . 216 00188273 Immune System Phenotyping 2 x Fast 96-well . . . . .212
00188193 T Regulatory Phenotyping 2 x 96-well . . . . . . . . . . . . 216 00188274 Immunological Targets of NFKB 2 x Fast 96-well . . . .212
00188194 Targets of Wnt/ß-catenin Signaling 2 x 96-well . . . . 216 00188275 Innate Immune Signaling 2 x Fast 96-well . . . . . . . . . .212
00188195 TGF-ß Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . 216 00188276 Interferon Signaling & Response 2 x Fast 96-well . . .212
00188196 Toll-like Receptor Signaling 2 x 96-well . . . . . . . . . . . . 216 00188277 Mood Disorder 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . .212
00188197 Wnt Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . 216 00188278 Neurodegeneration 2 x Fast 96-well . . . . . . . . . . . . . . .213
00188199 Alzheimer’s Disease 2 x 384-well . . . . . . . . . . . . . . . . . .214 00188279 NFKB Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . .213
00188200 Diabesity 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .214 00188280 Notch Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . .213
00188201 General Toxicology 2 x 384-well . . . . . . . . . . . . . . . . . . .214 00188281 Obesity 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . . . .213
00188202 Immunology 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . .215 00188282 Osteoporosis 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . .213
00188203 Lymphoma and Leukemia 2 x 384-well . . . . . . . . . . . .215 00188283 p53 Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . .213
00188204 Mood Disorder 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . .215 00188284 Parkinson’s 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . .213
00188205 Stem Cell 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . 216 00188285 Stress Response 2 x Fast 96-well . . . . . . . . . . . . . . . . .213
00188211 Alzheimer’s Disease 2 x 96-well . . . . . . . . . . . . . . . . . . 211 00188286 T cell Receptor Signaling 2 x Fast 96-well . . . . . . . . . .213
00188212 Antigen 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 00188287 T helper 17 (Th17) 2 x Fast 96-well . . . . . . . . . . . . . . . .213
00188213 Apoptosis 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 00188288 T Regulatory Phenotyping 2 x Fast 96-well . . . . . . . . .213
00188214 Autophagy 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 00188289 Targets of Wnt/ß-catenin Signaling 2 x Fast 96-well 213
00188215 B cell Receptor and Signaling 2 x 96-well . . . . . . . . . . 211 00188290 TGF-ß Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . .213
00188216 Blood Coagulation 2 x 96-well . . . . . . . . . . . . . . . . . . . . 211 00188291 Toll-like Receptor Signaling 2 x Fast 96-well . . . . . . . .213
Indices
00188217 Cell Cycle Tox and Cancer 2 x 96-well . . . . . . . . . . . . . 211 00188292 Wnt Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . .213
00188218 Cytokines and Receptors 2 x 96-well . . . . . . . . . . . . . 211 00188293 Alzheimer’s Disease 2 x 384-well . . . . . . . . . . . . . . . . . 211
00188219 DNA Damage and Repair 2 x 96-well . . . . . . . . . . . . . . .212 00188294 Antigen 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211
00188220 Electron Transport Toxicology 2 x 96-well . . . . . . . . . .212 00188295 Apoptosis 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . 211
00188231 FoxP3 Target Genes 2 x 96-well . . . . . . . . . . . . . . . . . . .212 00188296 Autophagy 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . 211
00188232 General Toxicology 2 x 96-well . . . . . . . . . . . . . . . . . . . .212 00188297 B cell Receptor and Signaling 2 x 384-well . . . . . . . . 211
00188233 Growth and Development Tox 2 x 96-well . . . . . . . . . . .212 00188298 Blood Coagulation 2 x 384-well . . . . . . . . . . . . . . . . . . . 211
00188234 Hematopoetic Cell Lineage 2 x 96-well . . . . . . . . . . . . .212 00188299 Human Cell Cycle Tox and Cancer 2 x 384-well . . . . . 211
344
Numerical Index
Continued
00188300 Cytokines and Receptors 2 x 384-well . . . . . . . . . . . . 211 00188394 Parkinson’s 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . 216
00188301 DNA Damage and Repair 2 x 384-well . . . . . . . . . . . . . .212 00188395 Pathogen Recognition 2 x Fast 96-well . . . . . . . . . . . . 216
00188302 Electron Transport Toxicology 2 x 384-well . . . . . . . . .212 00188396 Stress Response 2 x Fast 96-well . . . . . . . . . . . . . . . . 216
00188303 FoxP3 Target Genes 2 x 384-well . . . . . . . . . . . . . . . . . .212 00188397 T cell Receptor Signaling 2 x Fast 96-well . . . . . . . . . 216
00188304 General Toxicology 2 x 384-well . . . . . . . . . . . . . . . . . . .212 00188398 T helper 17 (Th17) 2 x Fast 96-well . . . . . . . . . . . . . . . 216
00188305 Growth and Development Tox 2 x 384-well . . . . . . . . .212 00188399 T Regulatory Phenotyping 2 x Fast 96-well . . . . . . . . 216
00188306 Hematopoetic Cell Lineage 2 x 384-well . . . . . . . . . . . .212 00188400 Targets of Wnt/ß-catenin Signaling 2 x Fast 96-well 216
00188307 Huntington’s Disease 2 x 384-well . . . . . . . . . . . . . . . . .212 00188401 TGF-ß Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . 216
00188308 Immune Complement 2 x 384-well . . . . . . . . . . . . . . . .212 00188402 Toll-like Receptor Signaling 2 x Fast 96-well . . . . . . . 216
00188309 Immune System Phenotyping 2 x 384-well . . . . . . . .212 00188403 Wnt Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . 216
00188310 Immunological Targets of NFKB 2 x 384-well . . . . . . . .212 00188424 Alzheimer’s Disease 2 x 384-well . . . . . . . . . . . . . . . . . .214
00188311 Innate Immune Signaling 2 x 384-well . . . . . . . . . . . . .212 00188425 Antigen Processing & Presentation 2 x 384-well . . . .214
00188312 Interferon Signaling & Response 2 x 384-well . . . . . .212 00188426 Apoptosis 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . .214
00188313 Mood Disorder 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . .212 00188427 Autophagy 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . .214
00188314 Neurodegeneration 2 x 384-well . . . . . . . . . . . . . . . . . .213 00188428 B cell Developmental and Function 2 x 384-well . . . .214
00188315 NFKB Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .213 00188429 B cell Receptor and Signaling 2 x 384-well . . . . . . . . .214
00188316 Notch Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .213 00188430 Blood Coagulation 2 x 384-well . . . . . . . . . . . . . . . . . . . .214
00188317 Obesity 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . .213 00188431 Cell Cycle Tox and Cancer 2 x 384-well . . . . . . . . . . . . .214
00188318 Osteoporosis 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . .213 00188432 Cytokines and Receptors 2 x 384-well . . . . . . . . . . . . .214
00188319 p53 Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . .213 00188433 DNA Damage and Repair 2 x 384-well . . . . . . . . . . . . . .214
00188320 Parkinson’s 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . .213 00188434 Electron Transport Toxicology 2 x 384-well . . . . . . . . .214
00188321 Stress Response 2 x 384-well . . . . . . . . . . . . . . . . . . . . .213 00188435 FoxP3 Target Genes 2 x 384-well . . . . . . . . . . . . . . . . . .214
00188322 T cell Receptor Signaling 2 x 384-well . . . . . . . . . . . . . .213 00188436 General Toxicology 2 x 384-well . . . . . . . . . . . . . . . . . . .214
00188323 T helper 17 (Th17) 2 x 384-well . . . . . . . . . . . . . . . . . . .213 00188437 Growth and Development Tox 2 x 384-well . . . . . . . . .215
00188324 Human T Regulatory Phenotyping 2 x 384-well . . . . .213 00188438 Hematopoetic Cell Lineage 2 x 384-well . . . . . . . . . . . .215
00188325 Targets of Wnt/ß-catenin Signaling 2 x 384-well . . . .213 00188439 Huntington’s Disease 2 x 384-well . . . . . . . . . . . . . . . . .215
00188326 TGF-ß Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .213 00188440 Immune Complement 2 x 384-well . . . . . . . . . . . . . . . .215
00188327 Toll-like Receptor Signaling 2 x 384-well . . . . . . . . . . .213 00188441 Immune System Phenotyping 2 x 384-well . . . . . . . .215
00188328 Wnt Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . .213 00188442 Immunological Targets of NFKB 2 x 384-well . . . . . . . .215
00188329 Alzheimer’s Disease 2 x 384-well . . . . . . . . . . . . . . . . . 211 00188443 Innate Immune Signaling 2 x 384-well . . . . . . . . . . . . .215
00188330 Diabesity 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . 211 00188444 Interferon Signaling & Response 2 x 384-well . . . . . .215
00188331 General Toxicology 2 x 384-well . . . . . . . . . . . . . . . . . . .212 00188445 Mood Disorder 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . .215
00188332 Immunology 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . .212 00188446 Natural Killer Cell 2 x 384-well . . . . . . . . . . . . . . . . . . . .215
00188333 Lymphoma and Leukemia 2 x 384-well . . . . . . . . . . . .212 00188447 Neurodegeneration 2 x 384-well . . . . . . . . . . . . . . . . . .215
00188334 Mood Disorder 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . .212 00188448 NFKB Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .215
00188335 Stem Cell 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .213 00188449 Notch Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .215
00188365 Alzheimer’s Disease 2 x Fast 96-well . . . . . . . . . . . . . .214 00188450 Obesity 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . 216
00188366 Antigen Processing & Presentation 2 x Fast 96-well 214 00188451 Osteoporosis 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . 216
00188367 Apoptosis 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . .214 00188452 p53 Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . 216
00188368 Autophagy 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . .214 00188453 Parkinson’s 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . . . 216
00188369 B cell Developmental and Function 2 x Fast 96-well 214 00188454 Pathogen Recognition 2 x 384-well . . . . . . . . . . . . . . . 216
00188370 B cell Receptor and Signaling 2 x Fast 96-well . . . . . .214 00188455 Stress Response 2 x 384-well . . . . . . . . . . . . . . . . . . . . 216
00188371 Blood Coagulation 2 x Fast 96-well . . . . . . . . . . . . . . . .214 00188456 T cell Receptor Signaling 2 x 384-well . . . . . . . . . . . . . 216
00188372 Cell Cycle Tox and Cancer 2 x Fast 96-well . . . . . . . . . .214 00188457 T helper 17 (Th17) 2 x 384-well . . . . . . . . . . . . . . . . . . 216
00188373 Cytokines and Receptors 2 x Fast 96-well . . . . . . . . . .214 00188458 T Regulatory Phenotyping 2 x 384-well . . . . . . . . . . . 216
00188374 DNA Damage and Repair 2 x Fast 96-well . . . . . . . . . .214 00188459 Targets of Wnt/ß-catenin Signaling 2 x 384-well . . . 216
00188375 Electron Transport Toxicology 2 x Fast 96-well . . . . . .214 00188460 TGF-ß Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . 216
00188376 FoxP3 Target Genes 2 x Fast 96-well . . . . . . . . . . . . . . .214 00188461 Toll-like Receptor Signaling 2 x 384-well . . . . . . . . . . 216
00188377 General Toxicology 2 x Fast 96-well . . . . . . . . . . . . . . . .214 00188462 Wnt Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . . . 216
00188378 Growth and Development Tox 2 x Fast 96-well . . . . . .215 00188657 AMPK Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . 211
00188379 Hematopoetic Cell Lineage 2 x Fast 96-well . . . . . . . .215 00188658 AMPK Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . 211
00188380 Huntington’s Disease 2 x Fast 96-well . . . . . . . . . . . . .215 00188659 AMPK Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . 211
00188381 Immune Complement 2 x Fast 96-well . . . . . . . . . . . . .215 00188660 AMPK Signaling 2 x 384-well . . . . . . . . . . . . . . . . . . . . . .214
00188382 Immune System Phenotyping 2 x Fast 96-well . . . . .215 00188661 AMPK Signaling 2 x 96-well . . . . . . . . . . . . . . . . . . . . . . .214
00188383 Immunological Targets of NFKB 2 x Fast 96-well . . . .215 00188662 AMPK Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . .214
00188384 Innate Immune Signaling 2 x Fast 96-well . . . . . . . . . .215 00189269 PowerCHO® -GS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268
00188385 Interferon Signaling & Response 2 x Fast 96-well . . .215 00189910 WinKQCL® 4 Software Package . . . . . . . . . . . . . . . . . . . 330
Indices
00188386 Mood Disorder 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . .215 04-351Q Schneider’s Drosophila 1 L . . . . . . . . . . . . . . . . . . . . . . . 259
00188387 Natural Killer Cell 2 x Fast 96-well . . . . . . . . . . . . . . . . .215 04-380Q X-VIVO™ 10 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
00188388 Neurodegeneration 2 x Fast 96-well . . . . . . . . . . . . . . .215 04-409R PBS-1X 15 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
00188389 NFKB Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . .215 04-418Q X-VIVO™ 15 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
00188390 Notch Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . .215 04-448Q X-VIVO™ 20 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
00188391 Obesity 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . . . . . . .215 04-457F Grace’s Insect Medium 500 ml . . . . . . . . . . . . . . . . . . . 255
00188392 Osteoporosis 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . . 216 04-479Q DPBS (1X) w/Ca++ and Mg++ 1 L . . . . . . . . . . . . . . . . . . . 286
00188393 p53 Signaling 2 x Fast 96-well . . . . . . . . . . . . . . . . . . . 216 04-501Q Grace’s Complete Insect 1 L . . . . . . . . . . . . . . . . . . . . . . 255
345
Numerical Index
Continued
04-525F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-167F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-585P Water for Cell Culture 20 L . . . . . . . . . . . . . . . . . . . . . . . 284 12-167F/12 RPMI 1640 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-616F TL HEPES Buffer 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 287 12-167F/24 RPMI 1640 24 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-648F Richter’s CM Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 258 12-167Q RPMI 1640 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-649F Grace’s Insect 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 255 12-167Q/12 RPMI 1640 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
04-719Q MEM Eagle Joklik 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-168F McCoy’s 5A 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
04-743Q X-VIVO™ 10 1 L w/o gentamicin or phenal red . . . . . . 265 12-169F MEM, Alpha 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
04-744Q X-VIVO™ 15 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265 12-604F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 253
04-919Q ProCHO™ 4 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267 12-604F/12 DMEM 4 .5 g/L Glucose 12 x 500ml . . . . . . . . . . . . . . . 253
06-174G MEM Eagle-EBSS 450 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-604Q DMEM 4 .5 g/L Glucose 1 L . . . . . . . . . . . . . . . . . . . . . . . 253
08-003A Hanks’ BSS (1X) 4 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282 12-604Q/12 DMEM 4 .5 g/L Glucose 12 x 1 L . . . . . . . . . . . . . . . . . . . 253
08-012B RPMI 1640 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-611F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
08-199D Water for Cell Culture 100 L . . . . . . . . . . . . . . . . . . . . . . 284 12-611F/12 MEM Eagle EBSS 12 x 500 ml . . . . . . . . . . . . . . . . . . . . 257
08-199E Water for Cell Culture 200 L . . . . . . . . . . . . . . . . . . . . . . 284 12-611Q MEM Eagle EBSS 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
08-199F Water for Cell Culture 20 L . . . . . . . . . . . . . . . . . . . . . . . 284 12-611Q/12 MEM Eagle EBSS 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . 257
08-E1322 Platinum UltraPAK™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-614F DMEM 4 .5 g/L Glucose 500ml . . . . . . . . . . . . . . . . . . . . 253
08-E1323 Platinum UltraPAK™ 5 L . . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-612F/12 DMEM 4 .5 g/L Glucose 12x 500ml . . . . . . . . . . . . . . . . 253
08-E1324 Platinum UltraPAK™ 10 L . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-614Q DMEM 4 .5 g/L Glucose 1 L . . . . . . . . . . . . . . . . . . . . . . . 253
08-E1325 Platinum UltraPAK™ 20 L . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-614Q/12 DMEM 4 .5 g/L Glucose 12x 1 L . . . . . . . . . . . . . . . . . . . 253
08-E269 Pillow Tank Liner 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-615F Ham’s F-12 Med w/L-Gln 500 ml . . . . . . . . . . . . . . . . . . 255
08-E270 Pillow Tank Liner 130 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-618F Ham’s F10 Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 255
08-E271 Pillow Tank Liner 200 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-624E Veronal Buffer (5X) 100 ml . . . . . . . . . . . . . . . . . . . . . . 287
08-E272 Pillow Tank Liner 340 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-662F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
08-E273 Pillow Tank Liner 400 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-662Q MEM Eagle EBSS 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
08-E275 Pillow Tank Liner 560 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-668E MEM Eagle EBSS (2X) 100 ml . . . . . . . . . . . . . . . . . . . . 257
08-E276 Pillow Tank Liner 790 L . . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-669E L-15 (Leibovitz) (2X) 100 ml . . . . . . . . . . . . . . . . . . . . . 256
08-E277 Pillow Tank Liner 1090 L . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-684F MEM Eagle EBSS (10X) 500 ml . . . . . . . . . . . . . . . . . . . 257
08-E278 Pillow Tank Liner 1360 L . . . . . . . . . . . . . . . . . . . . . . . . . 337 12-688F McCoy’s 5A 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
08-E279 ProEVA PAK™ 5 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 12-700F L-15 (Leibovitz) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 256
08-E280 ProEVA PAK™ 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 12-700Q L-15 (Leibovitz) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
08-E281 ProEVA PAK™ 20 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 12-702F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
08-E282 Gusseted Tank Liner 50 L . . . . . . . . . . . . . . . . . . . . . . . . 337 12-702F/12 RPMI 1640 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 259
08-E283 Gusseted Tank Liner 100 L . . . . . . . . . . . . . . . . . . . . . . . 337 12-702Q RPMI 1640 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
08-E284 Gusseted Tank Liner 200 L . . . . . . . . . . . . . . . . . . . . . . . 337 12-702Q/12 RPMI 1640 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
08-E287 ProEVA PAK™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 338 12-707F DMEM 1 .0 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254
08-E313 Platinum UltraPAK™ 50 L . . . . . . . . . . . . . . . . . . . . . . . . . 336 12-708F DMEM 1 .0 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254
08-E314 Platinum UltraPAK™ 100 L . . . . . . . . . . . . . . . . . . . . . . . . 336 12-709F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254
08-E315 Platinum UltraPAK™ 200 L . . . . . . . . . . . . . . . . . . . . . . . . 336 12-719F DMEM:F12 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 254
09-757F Pen/Strep Stock 500 ml . . . . . . . . . . . . . . . . . . . . 199, 285 12-719Q DMEM:F12 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 254
09-774E RPMI 1640 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-722F IMDM 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
09-774F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-722Q IMDM 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
10-508F Hanks’ BSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282 12-723B UltraDOMA™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 276
10-508Q Hanks’ BSS 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282 12-724Q UltraCHO™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 266
10-539B DGV Buffer 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 12-725F UltraCULTURE™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 262
10-548E ACK Lysing Buffer 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 286 12-726F IMDM 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-029Q ProCHO™ 4 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267 12-726F/12 IMDM 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-105F BME with Earle’s BSS 500 ml . . . . . . . . . . . . . . . . . . . . . 253 12-726Q IMDM 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-109F Medium199 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-726Q/12 IMDM 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-115F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-727F UltraDOMA™-PF™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 277
12-115F/12 RPMI 1640 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-730F Insect-XPRESS™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 278
12-115Q RPMI 1640 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-730Q Insect-XPRESS™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 278
12-115Q/12 RPMI 1640 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 12-733F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254
12-117F Medium199 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-733Q DMEM 4 .5 g/L Glucose 1 L . . . . . . . . . . . . . . . . . . . . . . . 254
12-117Q Medium199 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-736E MEM Eagle EBSS 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
12-118F Medium199 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-736F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
12-119F Medium 199 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 12-739F MEM, Glasgow’s 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 255
12-125F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
Indices
12-741F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 254

12-125F/12 MEM Eagle EBSS 12 x 500 ml . . . . . . . . . . . . . . . . . . . . 257 12-743F UltraMEM™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 264
12-125Q MEM Eagle EBSS 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-745F UltraMEM™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 264
12-125Q/12 MEM Eagle EBSS 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . 257 12-747F UltraSaline A 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
12-127F MEM Eagle HBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-749Q UltraMDCK™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 269
12-132A Cryoprotective Medium 100 ml . . . . . . . . . . . . . . . . . . 253 12-764Q Pro293™A-CDM™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 270
12-136F MEM Eagle EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-765Q Pro293™S-CDM™ 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 270
12-136Q MEM Eagle EBSS 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-766Q ProCHO™ 5 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267
12-137F MEM Eagle HBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257 12-769E ProFreeze™-CDM™ 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 269
346
Numerical Index
Continued
12-770Q PowerCHO®-1 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 17-516Q PBS (1X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
12-771Q PowerCHO®-2 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 17-516Q/12 PBS (1X) 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
12-772Q PowerCHO®-3 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268 17-517Q PBS (10X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
12-773Q ProNS0™ 1 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 17-518L Gentamicin Sulfate 50 mg/ml . . . . . . . . . . . . . . . 199, 285
12-774Q ProNS0™ 2 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 17-518Z Gentamicin Sulfate 50 mg/ml . . . . . . . . . . . . . . . 199, 285
12-775J ProNS0™ Lipid 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 279 17-519L Gentamicin Sulfate 10 mg/ml . . . . . . . . . . . . . . . 199, 285
12-914F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 253 17-519Z Gentamicin Sulfate 10 mg/ml . . . . . . . . . . . . . . . 199, 285
12-915F IMDM 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 255 17-528Z Gentamicin Sulfate 50 mg/ml . . . . . . . . . . . . . . . 199, 285
12-917F DMEM 4 .5 g/L Glucose 500 ml . . . . . . . . . . . . . . . . . . . 253 17-602E Pen/Strep stock 100 ml . . . . . . . . . . . . . . . . . . . . 199, 285
12-918F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259 17-602F Pen/Strep stock 500 ml . . . . . . . . . . . . . . . . . . . . 199, 285
12-923E NCTC-109 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 17-603E Pen/Strep stock 100 ml . . . . . . . . . . . . . . . . . . . . 199, 285
13-114E MEM NEAA 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283 17-605C L-glutamine 50 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
13-115E Sodium Pyruvate 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 283 17-605E L-glutamine 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
13-607C MEM Eagle Vitamin mix 50 ml . . . . . . . . . . . . . . . . . . . . 283 17-605F L-glutamine 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-401E Calf Serum 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-613E Sodium Bicarb . 7 .5% sol 100 ml . . . . . . . . . . . . . . . . . . 283
14-401F Calf Serum 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-711E Versene® (EDTA), 0 .02% 100 ml . . . . . . . . . . . . . . 46, 284
14-402E Human Serum 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-718R Pen/Strep/L-Gln mixture 25 x 4 .5 ml . . . . . . . . . 199, 285
14-403E Horse Serum, Donor 100 ml . . . . . . . . . . . . . . . . . . . . . . 294 17-719R Pen/Strep stock 25 x 4 .5 ml . . . . . . . . . . . . . . . . . 199, 285
14-403F Horse Serum, Donor 500 ml . . . . . . . . . . . . . . . . . . . . . . 294 17-724F Water for Cell Culture 500 ml . . . . . . . . . . . . . . . . . . . . . 284
14-416E Calf Serum, Newborn 100 ml . . . . . . . . . . . . . . . . . . . . . 295 17-724Q Water for Cell Culture 1 L . . . . . . . . . . . . . . . . . . . . . . . . . 284
14-416F Calf Serum, Newborn 500 ml . . . . . . . . . . . . . . . . . . . . . 295 17-737E HEPES Buffer 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
14-471E FBS Premium Irradiated 100 ml . . . . . . . . . . . . . . . . . . 294 17-737F HEPES Buffer 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
14-471F FBS Premium Irradiated 500 ml . . . . . . . . . . . . . . . . . . 294 17-745E Pen/Strep/Amphotericin B 100 ml . . . . . . . . . . . 199, 285
14-490E Human Serum AB 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 295 17-745H Pen/Strep/Amphotericin B 20 ml . . . . . . . . . . . . 199, 285
14-491E Human Serum AB, Male Plasma Derived 100 ml . . . 295 17-811A UltraCHO™ Supplement 100 ml . . . . . . . . . . . . . . . . . . . 266
14-492E Serum Supreme 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-829E Lymphocyte Separation 100 ml . . . . . . . . . . . . . . . . . . 283
14-492F Serum Supreme 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 295 17-829E/24 Lymphocyte Separation 24 x 100 ml . . . . . . . . . . . . . 283
14-498E Human Serum AB, Male 100 ml . . . . . . . . . . . . . . . . . . . 295 17-836E Amphotericin B,antifungal 100 ml . . . . . . . . . . . 199, 285
14-501E FBS Premium 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-836R Amphotericin B,antifungal 20 ml . . . . . . . . . . . . 199, 285
14-501F FBS Premium 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-838Z ITS 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-502E FBS USDA 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-839Z ITES 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-502F FBS USDA 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-905C L-glutamine 50 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-503E FBS Premium HI 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 294 17-942E Trypan Blue 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 283
14-503F FBS Premium HI 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 294 1A-125 Normal Peripheral Blood Leukopak100 ml . . . . . . . . . 83
14-507E FBS USDA HI 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 1M-101 Bone Marrow CD34+ 100,000 cells . . . . . . . . . . . . . . . . 86
14-507F FBS USDA HI 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 1M-101A Bone Marrow CD34+ 300,000 cells . . . . . . . . . . . . . . . . 86
14-901E FBS Premium Hybr 100 ml . . . . . . . . . . . . . . . . . . . . . . . 294 1M-101B Bone Marrow CD34+ 500,000 cells . . . . . . . . . . . . . . . . 86
14-901F FBS Premium Hybr 500 ml . . . . . . . . . . . . . . . . . . . . . . . 294 1M-101C Bone Marrow CD34+ 1 million cells . . . . . . . . . . . . . . . . 86
14-902E FBS Premium Chy 100 ml . . . . . . . . . . . . . . . . . . . . . . . . 294 1M-105 Human Bone Marrow, Fresh 10 ml . . . . . . . . . . . . . . . . . 83
15-456D DPBS 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 1M-125 Human Bone Marrow, Fresh 25 ml . . . . . . . . . . . . . . . . . 83
15-456F DPBS 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 1M-125A Bone Marrow Mono Cells 200 million cells . . . . . . . . . . 84
15-604D DMEM (Powder) 4 .5 g/L Glucose 10 L . . . . . . . . . . . . 253 1M-125C Bone Marrow Mono Cells 25 million cells . . . . . . . . . . . 84
15-605G L-glutamine Powder 25 g . . . . . . . . . . . . . . . . . . . . . . . . 283 1M-125D Bone Marrow Mono Cells 100 million cells . . . . . . . . . . 84
15-611D MEM Eagle EBSS 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . 257 1M-125E Bone Marrow Mono Cells 300 million cells . . . . . . . . . 84
15-613I Sodium Bicarbonate powder 500 g . . . . . . . . . . . . . . . 283 25-038 Aluminum Block w/lid . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
15-614D DMEM Powder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253 25-315 ELx808LBS Plate Reader . . . . . . . . . . . . . . . . . . . . . . . . 330
15-724D UltraCHO™ 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 266 25-340 LAL grade 96 well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
15-724F UltraCHO™ 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 266 25-342 Stepped Neutral Density Plate . . . . . . . . . . . . . . . . . . . 330
15-727D UltraDOMA™-PF Powder 10 L . . . . . . . . . . . . . . . . . . . . . . 277 25-344 FLx800LBS Fluorescent Plate Reader . . . . . . . . . . . . . 330
17-160E Trypsin(10x) 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284 25-364 Reagent reservoirs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
17-160F Trypsin(10x) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284 25-415 Eppendorf® Biopur Tips 200 µl . . . . . . . . . . . . . . . . . . . 330
17-161E Trypsin-Versene® (EDTA) 100 ml . . . . . . . . . . . . . . . . . 284 25-416 Eppendorf® Biopur Tips 300 µl . . . . . . . . . . . . . . . . . . . 330
17-161F Trypsin-Versene® (EDTA) 500 ml . . . . . . . . . . . . . . . . . 284 25-417 Eppendorf® Biopur Tips 1,000 µl . . . . . . . . . . . . . . . . . 330
17-512F DPBS (1X) 500 ml . . . . . . . . . . . . . . . . . . 63, 90 - 91 , 286 2C-101 Cord Blood CD34+ 1 million cells . . . . . . . . . . . . . . . . . . . 87
17-512F/12 DPBS (1X) 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-101A Cord Blood CD34+ 500,000 cells . . . . . . . . . . . . . . . . . . 87
17-512F/24 DPBS (1X) 24 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-101B Cord Blood CD34+ 100,000 cells . . . . . . . . . . . . . . . . . . . 87
17-512Q DPBS (1X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-102 Cord Blood CD133+ 500,000 cells . . . . . . . . . . . . . . . . . 87
Indices
17-512Q/12 DPBS (1X) 12 x 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-102A Cord Blood CD133+ 100,000 cells . . . . . . . . . . . . . . . . . 87
17-513F DPBS (1X) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-150 Cord Blood Mononuc Cells 200 million cells . . . . . . . . 84
17-513Q DPBS (1X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-150A Cord Blood Mononuc Cells 100 million cells . . . . . . . . 84
17-515F DPBS (10X) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-200 Cord Blood CD4+ T cells 20 million cells . . . . . . . . . . . . 95
17-515Q DPBS (10X) 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 2C-250 Cord Blood Erythroid Prog 1 million cells . . . . . . . . . . . 88
17-516F PBS (1X) 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46, 287 2C-300 Cord Blood CD19+ B cells 5 million cells . . . . . . . . . . . . 95
17-516F/12 PBS (1X) 12 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 287 2C-300A Cord Blood CD19+ B Cells 2 .5 million cells . . . . . . . . . . 95
17-516F/24 PBS (1X) 24 x 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 287 2C-300B Cord Blood CD19+ B Cells 1 million cells . . . . . . . . . . . . 95
347
Numerical Index
Continued
2L-101A Fetal Liver CD34+ 500,000 cells . . . . . . . . . . . . . . . . . . . 87 AWD-1001 Nucleofector® Guarantee, 1 year ext . . . . . . . . . . . . . . 134
2L-101B Fetal Liver CD34+ 100,000 cells . . . . . . . . . . . . . . . . . . . 87 AWD-1002 Nucleofector® Guarantee, 2 year ext . . . . . . . . . . . . . . 134
2L-102 Fetal Liver CD133+ 500,000 cells . . . . . . . . . . . . . . . . . . 87 AWM-1001 96-well Shuttle® Guarantee, 1 year ext . . . . . . . . . . . . 134
2L-102A Fetal Liver CD133+ 100,000 cells . . . . . . . . . . . . . . . . . . 87 AWM-1002 96-well Shuttle® Guarantee, 2 year ext . . . . . . . . . . . . 134
2M-101 Bone Marrow CD34+ 100,000 cells . . . . . . . . . . . . . . . . 86 AXA-1001 Nucleofector® 96-well Shuttle® System Rack . . . . . . 133
2M-101A Bone Marrow CD34+ 300,000 cells . . . . . . . . . . . . . . . . 86 BE02-002F MEM Alpha 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 257
2M-101B Bone Marrow CD34+ 500,000 cells . . . . . . . . . . . . . . . . 86 BE02-007E Trypsin/EDTA (10X) 100 ml . . . . . . . . . . . . . . . . . . . . . . 284
2M-101C Bone Marrow CD34+ 1 million cells . . . . . . . . . . . . . . . . 86 BE02-011F TC-100 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
2M-101D Bone Marrow CD34+ 2 million cells . . . . . . . . . . . . . . . . 86 BE02-012E Gentamicin Sulfate 100 ml . . . . . . . . . . . . . . . . . . 199, 285
2M-102 Bone Marrow CD133+ 500,000 cells . . . . . . . . . . . . . . . 87 BE02-014F Ham’s F10 Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 255
2M-102A Bone Marrow CD133+ 100,000 cells . . . . . . . . . . . . . . . 87 BE02-015BOX Lymphochrome™ 10 × 5 ml . . . . . . . . . . . . . . . . . . . . . . 272
2M-125A Bone Marrow Mono Cells 200 million . . . . . . . . . . . . . . 84 BE02-015E Lymphochrome™ 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 272
2M-125B Bone Marrow Mono Cells >25 million . . . . . . . . . . . . . . 84 BE02-015F Lymphochrome™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . 272
2M-125C Bone Marrow Mono Cells 25 million . . . . . . . . . . . . . . . . 84 BE02-016Q ProCHO™ AT 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267
2M-125D Bone Marrow Mono Cells 100 million . . . . . . . . . . . . . . . 84 BE02-017F PBS EDTA 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
2M-302 Bone Marrow Stromal Cells 5 million cells . . . . . . . . . . 89 BE02-018T PBS-ET 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 287
2S-101 Quadratox™ 4 Species Panel . . . . . . . . . . . . . . . . . . . . . . . 85 BE02-019F William’s Medium E 500 ml . . . . . . . . . . . . . . . . . . . . . . 259
2S-101A Baboon Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 BE02-020F EMEM with EBSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 257
2S-101B Canine Bone Marrow 5 million cells . . . . . . . . . . . . . . . . 85 BE02-026E Amniochrome® Plus 100 ml . . . . . . . . . . . . . . . . . . . . . . 273
2S-101C Murine Bone Marrow 5 million cells . . . . . . . . . . . . . . . . 85 BE02-026F Amniochrome® Plus 500 ml . . . . . . . . . . . . . . . . . . . . . 273
2S-101D Human Bone Marrow 5 million cells . . . . . . . . . . . . . . . 85 BE02-027F Earles BSS with HEPES 500 ml . . . . . . . . . . . . . . . . . . . 282
2T-110 Osteoclast Precusors 1 million cells . . . . . . . . . . . . . . . 92 BE02-028Q ProPer™ 1 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 271
2W-200 CD4+ Cells 10 million cells . . . . . . . . . . . . . . . . . . . . . . . . 95 BE02-029Q ProDoma™ 1 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 277
2W-400A CD14+ Cells 40 million cells . . . . . . . . . . . . . . . . . . . . . . . 93 BE02-030Q ProVero™ 1 serum-free medium, 1 L . . . . . . . . . . . . . . 270
2W-400B CD14+ Cells 20 million cells . . . . . . . . . . . . . . . . . . . . . . . 93 BE02-032Q ProDoma™ 3 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 277
2W-400C CD14+ Cells 10 million cells . . . . . . . . . . . . . . . . . . . . . . . 93 BE02-035E Amniochrome® Pro 100 ml . . . . . . . . . . . . . . . . . . . . . . . 273
2W-501 Human NK Cells neg selection . . . . . . . . . . . . . . . . . . . . 96 BE02-035F Amniochrome® Pro 500 ml . . . . . . . . . . . . . . . . . . . . . . 273
2W-502 Human NK Cells pos selection . . . . . . . . . . . . . . . . . . . . 96 BE02-036F HTF w/gentamicin 500 ml . . . . . . . . . . . . . . . . . . . . . . . 272
50-647U QCL-1000® 120 tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . 328 BE02-037F HTF HEPES w/gentamicin 500 ml . . . . . . . . . . . . . . . . 272
50-648U QCL-1000® 300 tests . . . . . . . . . . . . . . . . . . . . . . . . . . . . 328 BE02-038P20 Water for Cell Culture 20 L . . . . . . . . . . . . . . . . . . . . . . . 284
50-658U PyroGene®-rFC Kit 192 tests . . . . . . . . . . . . . . . . . . . . . 329 BE02-038P200 Water for Cell Culture 200 L . . . . . . . . . . . . . . . . . . . . . . 284
80-507L Sample Container 25/box . . . . . . . . . . . . . . . . . . . . . . . . 330 BE04-418F X-VIVO™ 15 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 265
80-507U Sample Container 100/box . . . . . . . . . . . . . . . . . . . . . . . 330 BE04-684E UltraGlutamine II 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 284
AAD-1001 Nucleofector® II . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 132 BE04-687F/U1 DMEM:F12 1:1 w/o HEPES 500ml . . . . . . . . . . . . . . . . 254
AAM-1001 Nucleofector® 96-well Shuttle® . . . . . . . . . . . . . . . . . . . 133 BE04-687Q DMEM:F12 1 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 254
AC-2509 bMVEC-B Brain MV Endo cryo amp . . . . . . . . . . . . . . . . . .13 BE10-502F Earle’s BSS 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282
AC-2621A Rat Hepatocytes 6 well plate . . . . . . . . . . . . . . . . . . . . . . .17 BE12-115E RPMI 1640 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-2622A Rat Hepatocytes 12 well plate . . . . . . . . . . . . . . . . . . . . . .17 BE12-115F/U1 RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-2623A Rat Hepatocytes 24 well plate . . . . . . . . . . . . . . . . . . . . . .17 BE12-604F/U1 DMEM 4 .5 g/L glucose, w/ UltraGlutamine 500 ml . 253
AC-2623B Rat Hepatocytes 24 well plate . . . . . . . . . . . . . . . . . . . . . .17 BE12-668F MEM Eagle EBSS (2X) 500 ml . . . . . . . . . . . . . . . . . . . . 257
AC-2624A Rat Hepatocytes 48 well plate . . . . . . . . . . . . . . . . . . . . .17 BE12-702F/U1 RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-2625A Rat Hepatocytes 96 well plate . . . . . . . . . . . . . . . . . . . . .17 BE12-723F UltraDOMA™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 276
AC-2627A Rat Hepatocytes T-25 flask . . . . . . . . . . . . . . . . . . . . . . . .17 BE12-738F Waymouth’s MB 752/1 500 ml . . . . . . . . . . . . . . . . . . . 259
AC-2628A Rat Hepatocytes T-75 flask . . . . . . . . . . . . . . . . . . . . . . . .17 BE12-752F RPMI 1640 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-2628B Rat Hepatocytes T-75 flask . . . . . . . . . . . . . . . . . . . . . . . .17 BE12-756EZM Amniochrome® II 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 273
AC-2630 Rat Hepatocytes suspension . . . . . . . . . . . . . . . . . . . . . .17 BE12-756FCM Amniochrome® II 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 273
AC-3103 EMVB Brain MV BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . .13 BE12-761F William’s Medium E 500 ml . . . . . . . . . . . . . . . . . . . . . . 259
AC-4207 EMVB Brain MV Endo Medium SingleQuots® . . . . . . . . .13 BE15-029D ProCHO™ 4 powder 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6001T25 bAEC T-25 flask, single donor . . . . . . . . . . . . . . . . . . . . . . 70 BE15-029F ProCHO™ 4 powder 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6001T75 bAEC T-75 flask, single donor . . . . . . . . . . . . . . . . . . . . . . 70 BE15-512D DPBS powder 1 × 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
AC-6001W96 bAEC 96 well plate, single donor . . . . . . . . . . . . . . . . . . . 70 BE15-512F DPBS powder 1 × 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . . 286
AC-6002T25 bAEC T-25 flask, pooled . . . . . . . . . . . . . . . . . . . . . . . . . . 70 BE15-604F DMEM (Powder) 4 .5 g/L glucose 50 L . . . . . . . . . . . . . 253
AC-6002T75 bAEC T-75 flask, pooled . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 BE15-702D RPMI 1640 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 259
AC-6002W96 bAEC 96 well plate, pooled . . . . . . . . . . . . . . . . . . . . . . . . 70 BE15-766D ProCHO™ 5 powder 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6004T25 bPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE15-766F ProCHO™ 5 powder 50 L . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6004T75 bPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE15-771D PowerCHO® 2 10 L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268
AC-6004W96 bPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE15-771J PowerCHO® 2 100L . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 268
Indices
AC-6005T25 bCAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE17-605E/U1 UltraGlutamine I 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 284

AC-6005T75 bCAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE17-855E ProHT™ Supplement 100 ml . . . . . . . . . . . . . . . . . . . . . . 283
AC-6005W96 bCAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 BE18-002Q ProMEDIA SELECT® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 267
AC-6009 Rat Hepatocytes cryo amp . . . . . . . . . . . . . . . . . . . . . . . .17 BW-6001 bAEC cryo amp, single donor . . . . . . . . . . . . . . . . . . . . . . 70
AWA-1001 Silver Service Contract Nucleofector® . . . . . . . . . . . . . 134 BW-6002 bAEC cryo amp, pooled . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
AWA-2002 Gold Service Contract Nucleofector® . . . . . . . . . . . . . . 134 BW-6004 bPAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
AWB-1001 Silver Service Contract 96-well Shuttle® . . . . . . . . . . 134 BW-6005 bCAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
AWB-2002 Gold Service Contract 96-well Shuttle® . . . . . . . . . . . . 134 C2501A NHEK-Ad cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
348
Numerical Index
Continued
C2501AT150 NHEK-Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2519AW6 HUVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2501AT225 NHEK-Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2519AW96 HUVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2501AT25 NHEK-Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AT150 HUAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AT75 NHEK-Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AT225 HUAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW12 NHEK-Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AT25 HUAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW24 NHEK-Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AT75 HUAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW48 NHEK-Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AW12 HUAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW6 NHEK-Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AW24 HUAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2501AW96 NHEK-Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 C2520AW48 HUAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2503A NHEK-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2520AW6 HUAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2503AT150 NHEK-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2520AW96 HUAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2503AT225 NHEK-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AT150 HPAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AT25 NHEK-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AT225 HPAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AT75 NHEK-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AT25 HPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW12 NHEK-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AT75 HPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW24 NHEK-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AW12 HPAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW48 NHEK-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AW24 HPAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW6 NHEK-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AW48 HPAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2503AW96 NHEK-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 42 C2530AW6 HPAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AT150 HMVEC-d Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530AW96 HPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AT225 HMVEC-d Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BT150 HPAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AT25 HMVEC-d Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BT225 HPAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AT75 HMVEC-d Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BT25 HPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW12 HMVEC-d Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BT75 HPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW24 HMVEC-d Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BW12 HPAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW48 HMVEC-d Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BW24 HPAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW6 HMVEC-d Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BW48 HPAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2505AW96 HMVEC-d Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 29 C2530BW6 HPAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507A NHEK-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530BW96 HPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AT150 NHEK-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CT150 HPAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AT225 NHEK-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CT225 HPAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AT25 NHEK-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CT25 HPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AT75 NHEK-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CT75 HPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW12 NHEK-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW12 HPAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW24 NHEK-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW24 HPAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW48 NHEK-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW48 HPAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW6 NHEK-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW6 HPAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2507AW96 NHEK-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 43 C2530CW96 HPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
C2516AT150 HMVEC-d Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AT150 HAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AT225 HMVEC-d Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AT225 HAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AT25 HMVEC-d Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AT25 HAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AT75 HMVEC-d Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AT75 HAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW12 HMVEC-d Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW12 HAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW24 HMVEC-d Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW24 HAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW48 HMVEC-d Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW48 HAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW6 HMVEC-d Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW6 HAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2516AW96 HMVEC-d Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 31 C2535AW96 HAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
C2517A HUVEC Cells, EGM®-2, cryo amp . . . . . . . . . . . . . . . . . . . 33 C2543AT150 HMVEC-d Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AT150 HUVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AT225 HMVEC-d Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AT225 HUVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AT25 HMVEC-d Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AT25 HUVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AT75 HMVEC-d Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AT75 HUVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW12 HMVEC-d Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW12 HUVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW24 HMVEC-d Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW24 HUVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW48 HMVEC-d Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW48 HUVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW6 HMVEC-d Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW6 HUVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 C2543AW96 HMVEC-d Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
C2517AW96 HUVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Indices
C2564AT150 UtMVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34

C2519A HUVEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 C2564AT225 UtMVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2519AT150 HUVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 C2564AT25 UtMVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2519AT225 HUVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 C2564AT75 UtMVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2519AT25 HUVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 C2564AW12 UtMVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2519AT75 HUVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 C2564AW24 UtMVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2519AW12 HUVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 C2564AW48 UtMVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2519AW24 HUVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 C2564AW6 UtMVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
C2519AW48 HUVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 C2564AW96 UtMVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
349
Numerical Index
Continued
C2585AT150 HCAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0285 NHBE T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
C2585AT225 HCAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0288 HMVEC-d Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 31
C2585AT25 HCAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0291 HIAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
C2585AT75 HCAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0294 SAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
C2585AW12 HCAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0297 NHA T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
C2585AW24 HCAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0310 PrEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
C2585AW48 HCAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0313 UtSMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
C2585AW6 HCAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0322 HUAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
C2585AW96 HCAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-0331 UtMVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
CC-0084 UtMVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2501 NHEK-Ad cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0085 HUAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC2501T150 NHEK-Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0088 PrEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC2501T225 NHEK-Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0089 UtSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2501W12 NHEK-Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0093 NHA 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 CC-2501W24 NHEK-Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0094 SAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2501W48 NHEK-Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0095 HIAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2501W6 NHEK-Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
CC-0096 CASMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2503 NHEK-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0100 NHBE 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 CC2503T150 NHEK-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0104 NHEK-Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 CC2503T225 NHEK-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0108 NHEK-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 42 CC-2503W12 NHEK-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0112 HMVEC-d Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 29 CC-2503W24 NHEK-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0116 NHDF-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2503W48 NHEK-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0124 HUVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2503W6 NHEK-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
CC-0128 HPAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2504 NHEM-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0132 HAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2504T150 NHEM-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0136 NHBE 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2504T225 NHEM-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0140 HMEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2504T25 NHEM-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0144 SkMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2504T75 NHEM-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0148 AoSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 CC-2504W12 NHEM-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0152 PASMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 CC-2504W24 NHEM-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0156 NHEK-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 43 CC-2504W48 NHEM-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0160 NHDF-Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2504W6 NHEM-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0164 NHLF 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2504W96 NHEM-Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-0168 RPTEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2505 HMVEC-d Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0172 HRCE 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2505T150 HMVEC-d Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0176 NHMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2505T225 HMVEC-d Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0180 BSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2505W12 HMVEC-d Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0184 HMVEC-L 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2505W24 HMVEC-d Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0188 HCAEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2505W48 HMVEC-d Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0192 UASMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2505W6 HMVEC-d Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-0201 NHEK-Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 CC-2507 NHEK-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0204 NHEK-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 CC2507T150 NHEK-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0207 HMVEC-d Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 CC2507T225 NHEK-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0210 NHDF-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2507W12 NHEK-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0216 HUVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2507W24 NHEK-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0219 HPAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2507W48 NHEK-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0222 HAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2507W6 NHEK-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
CC-0225 NHBE T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2508 PrSC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0228 HMEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC2508T150 PrSC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0231 SkMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC2508T225 PrSC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0234 AoSMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 CC-2508T75 PrSC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0237 PASMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 CC-2508W12 PrSC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0240 BSMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2508W24 PrSC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0243 UASMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2508W48 PrSC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0246 HMVEC-d Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29 CC-2508W6 PrSC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0252 NHDF-Ad T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2508W96 PrSC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
CC-0255 NHEK-Neo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
Indices
CC-2509 NHDF-Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38

CC-0258 CASMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC2509T150 NHDF-Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
CC-0261 HCAEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC2509T225 NHDF-Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
CC-0264 HMVEC-L T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2509W12 NHDF-Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
CC-0267 RPTEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2509W24 NHDF-Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
CC-0270 HRCE T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2509W48 NHDF-Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 38
CC-0273 NHMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2509W6 NHDF-Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
CC-0276 HUVEC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2511 NHDF-Ad cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
CC-0282 NHLF T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC2511T150 NHDF-Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
350
Numerical Index
Continued
CC2511T225 NHDF-Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2533W96 BdSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65
CC-2511W12 NHDF-Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2535 HAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2511W24 NHDF-Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC2535T150 HAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2511W48 NHDF-Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC2535T225 HAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2511W6 NHDF-Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2535W12 HAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2512 NHLF cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2535W24 HAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC2512T150 NHLF T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2535W48 HAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC2512T225 NHLF T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2535W6 HAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2512W12 NHLF 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2538 NHOst cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2512W24 NHLF 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC2538T150 NHOst T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2512W48 NHLF 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC2538T225 NHOst T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2512W6 NHLF 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2538T25 NHOst T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516 HMVEC-d Neo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538T75 NHOst T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC2516T150 HMVEC-d Neo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W12 NHOst 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC2516T225 HMVEC-d Neo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W24 NHOst 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516W12 HMVEC-d Neo 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W48 NHOst 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516W24 HMVEC-d Neo 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W6 NHOst 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516W48 HMVEC-d Neo 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2538W96 NHOst 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2516W6 HMVEC-d Neo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2540 NHBE cryo . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2516W96 HMVEC-d Neo 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 31 CC2540T150 NHBE T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2517 HUVEC Cells, EGM®, cryo amp . . . . . . . . . . . . . . . . . . . . . 33 CC2540T225 NHBE T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC2517T150 HUVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2540W12 NHBE 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC2517T225 HUVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2540W24 NHBE 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2517W12 HUVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2540W48 NHBE 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2517W24 HUVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2540W6 NHBE 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2517W48 HUVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2541 NHBE cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2517W6 HUVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC2541T150 NHBE T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2519 HUVEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC2541T225 NHBE T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC2519T150 HUVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2541W12 NHBE 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC2519T225 HUVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2541W24 NHBE 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2519W12 HUVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2541W48 NHBE 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2519W24 HUVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2541W6 NHBE 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-2519W48 HUVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2543 HMVEC-d Ad cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2519W6 HUVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC2543T150 HMVEC-d Ad T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2519W96 HUVEC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC2543T225 HMVEC-d Ad T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2520 HUAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W12 HMVEC-d Ad 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC2520T150 HUAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W24 HMVEC-d Ad 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC2520T225 HUAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W48 HMVEC-d Ad 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2520W12 HUAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W6 HMVEC-d Ad 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2520W24 HUAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2543W96 HMVEC-d Ad 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2520W48 HUAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2545 HIAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2520W6 HUAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC2545T150 HIAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2527 HMVEC-L cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC2545T225 HIAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC2527T150 HMVEC-L T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2545W12 HIAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC2527T225 HMVEC-L T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2545W24 HIAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2527W12 HMVEC-L 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2545W48 HIAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2527W24 HMVEC-L 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2545W6 HIAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
CC-2527W48 HMVEC-L 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2547 SAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2527W6 HMVEC-L 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC2547T150 SAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2530 HPAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC2547T225 SAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC2530T150 HPAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2547W12 SAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC2530T225 HPAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2547W24 SAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2530W12 HPAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2547W48 SAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2530W24 HPAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2547W6 SAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
CC-2530W48 HPAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2550 NHAC-kn cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2530W6 HPAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC2550T150 NHAC-kn T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2533 BdSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65
Indices
CC2550T225 NHAC-kn T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59

CC2533T150 BdSMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 CC-2550T25 NHAC-kn T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC2533T225 BdSMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 CC-2550T75 NHAC-kn T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2533T25 BdSMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 CC-2550W12 NHAC-kn 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2533T75 BdSMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 CC-2550W24 NHAC-kn 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2533W12 BdSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 CC-2550W48 NHAC-kn 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2533W24 BdSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 CC-2550W6 NHAC-kn 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2533W48 BdSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 CC-2550W96 NHAC-kn 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
CC-2533W6 BdSMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 CC-2551 HMEC cryoamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
351
Numerical Index
Continued
CC2551T150 HMEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2565 NHA Cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC2551T225 HMEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC2565T150 NHA T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2551W12 HMEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC2565T225 NHA T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2551W24 HMEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2565W12 NHA 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2551W48 HMEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2565W24 NHA 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2551W6 HMEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2565W48 NHA 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC-2553 RPTEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2565W6 NHA 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
CC2553T150 RPTEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2571 AoSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC2553T225 RPTEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2571T150 AoSMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2553W12 RPTEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2571T225 AoSMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2553W24 RPTEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2571W12 AoSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2553W48 RPTEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2571W24 AoSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2553W6 RPTEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2571W48 AoSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC-2554 HRCE cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2571W6 AoSMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
CC2554T150 HRCE T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2576 BSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC2554T225 HRCE T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2576T150 BSMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2554W12 HRCE 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2576T225 BSMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2554W24 HRCE 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2576W12 BSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2554W48 HRCE 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2576W24 BSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2554W6 HRCE 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2576W48 BSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2555 PrEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2576W6 BSMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC2555T150 PrEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2579 UASMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC2555T225 PrEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC2579T150 UASMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2555W12 PrEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC2579T225 UASMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2555W24 PrEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2579W12 UASMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2555W48 PrEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2579W24 UASMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2555W6 PrEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2579W48 UASMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC-2556 HRE cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2579W6 UASMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
CC2556T150 HRE T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580 HSMM cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC2556T225 HRE T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2580T150 NHAC-kn T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556T25 HRE T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC2580T225 NHAC-kn T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556T75 HRE T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580T25 HSMM T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W12 HRE 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580T75 HSMM T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W24 HRE 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580W12 HSMM 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W48 HRE 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580W24 HSMM 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W6 HRE 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580W48 HSMM 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2556W96 HRE 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2580W6 HSMM 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC-2559 NHMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2580W96 HSMM 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
CC2559T150 NHMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2581 PASMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC2559T225 NHMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2581T150 PASMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2559W12 NHMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC2581T225 PASMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2559W24 NHMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2581W12 PASMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2559W48 NHMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2581W24 PASMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2559W6 NHMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2581W48 PASMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC-2561 SkMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2581W6 PASMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
CC2561T150 SkMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2583 CASMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC2561T225 SkMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC2583T150 CASMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2561W12 SkMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC2583T225 CASMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2561W24 SkMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2583W12 CASMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2561W48 SkMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2583W24 CASMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2561W6 SkMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2583W48 CASMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC-2562 UtSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2583W6 CASMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
CC2562T150 UtSMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2585 HCAEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC2562T225 UtSMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC2585T150 HCAEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2562W12 UtSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC2585T225 HCAEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2562W24 UtSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2585W12 HCAEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2562W48 UtSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Indices
CC-2585W24 HCAEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

CC-2562W6 UtSMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2585W48 HCAEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC-2564 UtMVEC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2585W6 HCAEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25
CC2564T150 UtMVEC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2586 NHEM-Ad cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC2564T225 UtMVEC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2587 PrSMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67
CC-2564W12 UtMVEC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC2587T150 PrSMC T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67
CC-2564W24 UtMVEC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC2587T225 PrSMC T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67
CC-2564W48 UtMVEC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2587T25 PrSMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67
CC-2564W6 UtMVEC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2587T75 PrSMC T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67
352
Numerical Index
Continued
CC-2587W12 PrSMC 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810 HMVEC-dLyAd cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2587W24 PrSMC 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810T150 HMVEC-dLyAd T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2587W48 PrSMC 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810T225 HMVEC-dLyAd T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2587W6 PrSMC 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810T25 HMVEC-dLyAd T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2587W96 PrSMC 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 CC-2810T75 HMVEC-dLyAd T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2591 h NHEPS® cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 CC-2810W12 HMVEC-dLyAd 12 well plate . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2601 NHEK-Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 CC-2810W24 HMVEC-dLyAd 24 well plate . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2603 NHEK-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 CC-2810W48 HMVEC-dLyAd 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2605 HMVEC-d Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29 CC-2810W6 HMVEC-dLyAd 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2607 NHEK-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 CC-2810W96 HMVEC-dLyAd 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 28
CC-2608 PrSC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2811 HMVEC-dBlAd cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2609 NHDF-Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2811T150 HMVEC-dBlAd T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2611 NHDF-Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 CC-2811T225 HMVEC-dBlAd T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2612 NHLF T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-2811T25 HMVEC-dBlAd T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2616 HMVEC-d Neo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 31 CC-2811T75 HMVEC-dBlAd T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2617 HUVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2811W12 HMVEC-dBlAd 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2619 HUVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2811W24 HMVEC-dBlAd 24 well plate . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2620 HUAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 CC-2811W48 HMVEC-dBlAd 48 well plate . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2627 HMVEC-L T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2811W6 HMVEC-dBlAd 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2630 HPAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 CC-2811W96 HMVEC-dBlAd 96 well plate . . . . . . . . . . . . . . . . . . . . . . . 29
CC-2635 HAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2812 HMVEC-dLyNeo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2640 NHBE T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2812T150 HMVEC-dLyNeo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2641 NHBE T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 CC-2812T225 HMVEC-dLyNeo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2643 HMVEC-d Ad T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 CC-2812T25 HMVEC-dLyNeo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2644 UtMVEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 CC-2812T75 HMVEC-dLyNeo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2645 HIAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 CC-2812W12 HMVEC-dLyNeo 12 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2647 SAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 CC-2812W24 HMVEC-dLyNeo 24 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2651 HMEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-2812W48 HMVEC-dLyNeo 48 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2653 RPTEC T-25 Flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2812W6 HMVEC-dLyNeo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2654 HRCE T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 CC-2812W96 HMVEC-dLyNeo 96 well plate . . . . . . . . . . . . . . . . . . . . . 30
CC-2655 PrEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 CC-2813 HMVEC-dBlNeo cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2659 NHMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 CC-2813T150 HMVEC-dBlNeo T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2661 SkMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 CC-2813T225 HMVEC-dBlNeo T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2662 UtSMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2813T25 HMVEC-dBlNeo T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2665 NHA T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 CC-2813T75 HMVEC-dBlNeo T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2671 AoSMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 CC-2813W12 HMVEC-dBlNeo 12 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2676 BSMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2813W24 HMVEC-dBlNeo 24 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2679 UASMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 CC-2813W48 HMVEC-dBlNeo 48 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2681 PASMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 CC-2813W6 HMVEC-dBlNeo 6 well plate . . . . . . . . . . . . . . . . . . . . . . . 30
CC-2683 CASMC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 CC-2813W96 HMVEC-dBlNeo 96 well plate . . . . . . . . . . . . . . . . . . . . . . 30
CC-2685 HCAEC T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 CC-2814 HMVEC-Lly cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2690 h NHEPS® suspension . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 CC-2814T150 HMVEC-Lly T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2691A h NHEPS® collagen, 6 well plate . . . . . . . . . . . . . . . . . . . .15 CC-2814T225 HMVEC-Lly T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2691B h NHEPS® Matrigel®, 6 well plate . . . . . . . . . . . . . . . . . . .15 CC-2814T25 HMVEC-Lly T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2692A h NHEPS® collagen, 12 well plate . . . . . . . . . . . . . . . . . . .15 CC-2814T75 HMVEC-Lly T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2692B h NHEPS® Matrigel®, 12 well plate . . . . . . . . . . . . . . . . . .15 CC-2814W12 HMVEC-Lly 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2693A h NHEPS® collagen, 24 well plate . . . . . . . . . . . . . . . . . . .15 CC-2814W24 HMVEC-Lly 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2693B h NHEPS® Matrigel®, 24 well plate . . . . . . . . . . . . . . . . . .15 CC-2814W48 HMVEC-Lly 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2694 h NHEPS® collagen, T-25 flask . . . . . . . . . . . . . . . . . . . . . .15 CC-2814W6 HMVEC-Lly 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2694B h NHEPS® Matrigel®, T-25 flask . . . . . . . . . . . . . . . . . . . . .15 CC-2814W96 HMVEC-Lly 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2695 h NHEPS® collagen, T-75 flask . . . . . . . . . . . . . . . . . . . . . .15 CC-2815 HMVEC-LBl cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2695B h NHEPS® Matrigel®, T-75 flask . . . . . . . . . . . . . . . . . . . . .15 CC-2815T150 HMVEC-LBl T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2696 h NHEPS® collagen, T-150 flask . . . . . . . . . . . . . . . . . . . . .15 CC-2815T225 HMVEC-LBl T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2696B h NHEPS® Matrigel®, T-150 flask . . . . . . . . . . . . . . . . . . . .15 CC-2815T25 HMVEC-LBl T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2698A h NHEPS® collagen,96 well plate . . . . . . . . . . . . . . . . . . .15 CC-2815T75 HMVEC-LBl T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2698B h NHEPS® Matrigel®, 96 well plate . . . . . . . . . . . . . . . . . .15 CC-2815W12 HMVEC-LBl 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2701 NHDC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
Indices
CC-2815W24 HMVEC-LBl 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27

CC-2702 HPBMC cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 94 CC-2815W48 HMVEC-LBl 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2703T25 Ready Heps™, T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . .16 CC-2815W6 HMVEC-LBl 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2703T75 Ready Heps™, T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . .16 CC-2815W96 HMVEC-LBl 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 27
CC-2703W12 Ready Heps™, 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . .16 CC-3001 KGM® Complete Medium 500 ml . . . . . . . . . . . . . . . . . . . 44
CC-2703W24 Ready Heps™, 24 well plate . . . . . . . . . . . . . . . . . . . . . . . .16 CC-3024 EGM® Complete Medium 500 ml . . . . . . . . . . . . . . . . . . . 35
CC-2703W48 Ready Heps™, 48 well plate . . . . . . . . . . . . . . . . . . . . . . . .16 CC-3051 MEGM® Complete Medium 500 ml . . . . . . . . . . . . . . . . . 48
CC-2703W6 Ready Heps™, 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . .16 CC-3101 KBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 44
CC-2703W96 Ready Heps™, 96 well plate . . . . . . . . . . . . . . . . . . . . . . . .16 CC-3103 KBM®-2 Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . 44
353
Numerical Index
Continued
CC-3104 KBM® Basal Medium w/o Ca++ 500 ml . . . . . . . . . . . . . . 44 CC-3275 RCBM Basal Medium, 200ml . . . . . . . . . . . . . . . . . . . . . . .72
CC-3107 KGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 -44 CC-4009 Bovine Pit . Extract (BPE) 2 ml . . . . . . . . . . . . . . . . . . . . 78
CC-3108 KGM®-2 BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . 44 CC-4068 hFGF 1 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3111 KGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . .41, 42, 43, 44 CC-4098 Bovine Brain Ext . (BBE) 5 ml . . . . . . . . . . . . . . . . . . . . . . 78
CC-3112 KGM® BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . 44 CC-4107 hEGF 3 µg/mi 0 .5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3118 SAGM™ BulletKit® (CC-3119 and CC-4124) . . . . . . . 19, 20 CC-4123 AGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 50, 77
CC-3119 SABM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 20 CC-4124 SAEC SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-3121 EBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 35 CC-4126 FGM®-2 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 39
CC-3124 EGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . 33, 34, 35 CC-4127 REGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . 11, 57
CC-3125 EGM®-MV BulletKit® . . . . . . . . . . . . . . . . . . . . 33, 35, 70, 71 CC-4131 KGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
CC-3129 EBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 35 CC-4133 EGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
CC-3130 FGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 CC-4134 FGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
CC-3131 FBM® Fibroblast Basal 500 ml . . . . . . . . . . . . . . . . . . . . . 39 CC-4136 MEGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 48
CC-3132 FGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38, 39 CC-4139 SkGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 63
CC-3146 MsGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56, 57 CC-4143 EGM®-MV SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 35
CC-3147 MsBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 57 CC-4146 MsGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 57
CC-3150 MEGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 CC-4147 EGM®-2 MV SingleQuots® Kit . . . . . . . . . . . . . . . . . . . 22, 35
CC-3151 MEBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 48 CC-4149 SmGM®-2 SingleQuots® Kit . . . . . . . . . . . . . .22, 53, 65, 68
CC-3152 MEBM® Basal CC-4152 KGM®-2 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 44
Sodium Bicarbinate-free Medium 500 ml . . . . . . . . . 48 CC-4175 BEGM® SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 20
CC-3153 MEBM® Basal Phenol Red-free Medium 500 ml . . . . . 48 CC-4176 EGM®-2 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 35
CC-3156 EBM®-2 Basal Medium 500 ml . . . . . . . . . . . . . 13, 22, 35 CC-4177 PrEGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . 53, 67
CC-3158 KBM®-2 Basal Medium w/o Ca++ 500 ml . . . . . . . . . . . . 44 CC-4181 SCGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . 39, 53
CC-3160 SkGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 - 63 CC-4182 HCM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 15, 17
CC-3161 SkBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 63 CC-4192 HMM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 15, 17
CC-3162 EGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . 25, 32, 33, 34, 35 CC-4193 OGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
CC-3165 PrEBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . 53, 67 CC-4194 OGM™ Diff . SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . 60
CC-3166 PrEGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . 52, 53, 67 CC-4202 Calcium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3170 BEGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19, 20 CC-4205 Human Transferrin 0 .5 ml . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3171 BEBM® Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . 20 CC-4241 Neural Prog . Maint . Med . . . . . . . . . . . . . . . . . . . . . . . . . . 97
CC-3181 SmBM® Basal Medium 500 ml . . . . . . . . . .22, 53, 65, 68 CC-4242 Neural Progenitor Suppl . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
CC-3182 SmGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . 22, 53, 65 - 68 CC-4323 NSF-1 4 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
CC-3186 AGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . 50, 75 - 77 CC-4398 Ascorbic Acid 0 .5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 60, 78
CC-3187 ABM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . 50, 77 CC-4408 CDM SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
CC-3190 REGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . 11, 55 - 57 CC-4409 CGM SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
CC-3191 REBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . 11, 57 CC-4435 MGM®-4 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 46
CC-3197 HMM™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15, 17 CC-4444 Corneal Model BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
CC-3198 HCM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15, 17 CC-4455 KGM®-CD BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
CC-3199 HBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . 15, 17 CC-4456 KGM®-CD SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 44
CC-3202 EGM®-2 MV BulletKit® . . . . . . . . . . . . . .22, 25 - 31, 34, 35 CC-4461 PNGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . .74, 76, 77
CC-3204 SCBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . 39, 53 CC-4462 PNGM™ SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . 77
CC-3205 SCGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . 37, 39, 52, 53 CC-4510 Endothelin-3 (ET-3), 130 µg . . . . . . . . . . . . . . . . . . . . . . . 46
CC-3207 OGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59, 60 CC-4511 PNGM™-A SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . 77
CC-3208 OBM™ Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . 60 CC-4512 PNGM™-A BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . 74, 77
CC-3209 NPMM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 CC-4515 RCGM BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72
CC-3210 NPBM™ Medium 200 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 CC-4516 RCGM SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . .72
CC-3211 LGM-3® Growth Medium 500 ml . . . . . . . . . . . . . . . . . . . 94 CC-5002 Trypsin Neutralizing Soln 100 ml . . . . . . . . . . . . . . . 46, 78
CC-3216 CGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 - 60 CC-5012 Trypsin/EDTA Solution 100 ml . . . . . . . . . . . . . . . . . . 46, 78
CC-3217 CBM Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . . . . 60 CC-5022 HEPES Buffered Saline 100 ml . . . . . . . . . . . . . . . . . . . . 78
CC-3225 CDM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 - 60 CC-5024 HEPES Buffered Saline 500 ml . . . . . . . . . . . . . . . . . . . . 78
CC-3226 CDM™ Basal Medium 250 ml . . . . . . . . . . . . . . . . . . . . . . . 60 CC-5034 ReagentPack™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
CC-3229 NPDM BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 . . . . 20, 22, 35, 39, 44, 48, 50, 53, 57, 60, 63, 68, 77, 78
CC-3232 Trypsin/EDTA for MSC 100 ml . . . . . . . . . . . . . . . . . . . . . . 90 CC-7014 AoAF cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3233 Chondrocyte ReagentPack™ . . . . . . . . . . . . . . . . . . . . . . . 60 CC7014T150 AoAF T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3234 Sodium Alginate 50 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 CC7014T225 AoAF T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3235 Calcium Chloride Solution 500 ml . . . . . . . . . . . . . . . . . 60 CC-7014T25 AoAF T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3236 Sodium Chloride Solution 500 ml . . . . . . . . . . . . . . . . . . 60 CC-7014T75 AoAF T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Indices
CC-3237 Sodium Citrate Solution 100 ml . . . . . . . . . . . . . . . . . . . 60 CC-7014W12 AoAF 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37

CC-3244 SkGM®-2 SingleQuots® Kits . . . . . . . . . . . . . . . . . . . . . . . 63 CC-7014W24 AoAF 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3245 SkGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . 62 - 63 CC-7014W48 AoAF 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3246 SkBM®-2 Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . 63 CC-7014W6 AoAF 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3249 MGM®-4 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 CC-7014W96 AoAF 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
CC-3250 MBM®-4 Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . . 46 CC-7016 HMVEC-Bd cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
CC-3255 KBM®-CD Basal Medium 500 ml . . . . . . . . . . . . . . . . . . . 44 CC7016T150 HMVEC-Bd T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
CC-3256 PNGM™ Basal Medium 200 ml . . . . . . . . . . . . . . . . . . . . . 77 CC7016T225 HMVEC-Bd T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
354
Numerical Index
Continued
CC-7016T25 HMVEC-Bd T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-418 MycoAlert® Kit 50 Tests . . . . . . . . . . . . . . . . . . . . . . . . . 220
CC-7016T75 HMVEC-Bd T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-500 PKLight® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230
CC-7016W12 HMVEC-Bd 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-501 PKLight® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230
CC-7016W24 HMVEC-Bd 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-517 ToxiLight® Control 10 ml . . . . . . . . . . . . . . . . . . . . . . . . . 226
CC-7016W48 HMVEC-Bd 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-518 MycoAlert® Control Set 10 Tests . . . . . . . . . . . . . . . . . . 220
CC-7016W6 HMVEC-Bd 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-600 PDELight® Kit 500 tests . . . . . . . . . . . . . . . . . . . . . . . . . 232
CC-7016W96 HMVEC-Bd 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . 22 LT07-610 PPiLight™ Assay 500 tests . . . . . . . . . . . . . . . . . . . . . . . 234
CC-7030 HMVEC-C cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT07-818 MycoZap™ 1 Treatment . . . . . . . . . . . . . . . . . . . . . 198, 222
CC-7030T150 HMVEC-C T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT07-918 MycoZap™ 5 Treatments . . . . . . . . . . . . . . . . . . . . 198, 222
CC-7030T225 HMVEC-C T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT17-217 ToxiLight® Assay Kit with, 500 tests . . . . . . . . . . . . . . 226
CC-7030T25 HMVEC-C T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT17-221 ViaLight® Plus 500 tests . . . . . . . . . . . . . . . . . . . . . . . . . 223
CC-7030T75 HMVEC-C T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT27-008 ATP Standard 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 241
CC-7030W12 HMVEC-C 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT27-040 ExPro™ Cleaning Solution 50 ml . . . . . . . . . . . . . . . . . . 241
CC-7030W24 HMVEC-C 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 LT27-102 Tissue Culture Plate, White . . . . . . . . . . . . . . . . . . . . . . . 241
CC-7030W48 HMVEC-C 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 M-AsM-330 Mouse C57 Mixed cryo amp . . . . . . . . . . . . . . . . . . . . . . . 76
CC-7030W6 HMVEC-C 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 M-AsM-430 Mouse CD1 Mixed Astrocyte cryo amp . . . . . . . . . . . . . 76
CC-7030W96 HMVEC-C 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31 M-Cp-302 Mouse C57 Striatum Neuron cryo amp . . . . . . . . . . . . . 76
CC-7049 HPdLF cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 M-Cp-402 Mouse CD1 Striatum Neuron cryo amp . . . . . . . . . . . . . 76
CC-7049T150 HPdLF T-150 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 M-Cx-300 Mouse C57 Cortex Neurons cryo amp . . . . . . . . . . . . . . 76
CC-7049T225 HPdLF T-225 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 M-Cx-400 Mouse CD1 Cortex Neurons cryo amp . . . . . . . . . . . . . . 76
CC-7049T25 HPdLF T-25 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N183-06 PYROGENT® 80 test kit 0 .06 . . . . . . . . . . . . . . . . . . . . . 326
CC-7049T75 HPdLF T-75 flask . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N183-125 PYROGENT® 80 test kit 0 .125 . . . . . . . . . . . . . . . . . . . . . 326
CC-7049W12 HPdLF 12 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N184-25 PYROGENT® 250 test kit 0 .25 . . . . . . . . . . . . . . . . . . . . 326
CC-7049W24 HPdLF 24 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N185 Endotoxin (E.coli 055:B5) . . . . . . . . . . . . . . . . . . . . . . . 327
CC-7049W48 HPdLF 48 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N186 Endotoxin (E.coli 055:B5) . . . . . . . . . . . . . . . . . . . . . . . 327
CC-7049W6 HPdLF 6 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N188 Pyrosperse® Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
CC-7049W96 HPdLF 96 well plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 N189-06 PYROGENT®-25 SingleTest Vial 0 .06 . . . . . . . . . . . . . . 326
CMS-2000 Renal Epithelial Cell Model . . . . . . . . . . . . . . . . . . . . . . . . 11 N189-125 PYROGENT®-25 SingleTest Vial 0 .125 . . . . . . . . . . . . . . 326
CMS-2015 Corneal Epithelial Model . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 N189-25 PYROGENT®-25 SingleTest Vial 0 .25 . . . . . . . . . . . . . . . 326
DE14-417E Calf Serum Newborn 100 ml . . . . . . . . . . . . . . . . . . . . . 295 N190 b-G-Blocker™ 5 × 5 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
DE14-417F Calf Serum Newborn 500 ml . . . . . . . . . . . . . . . . . . . . . 295 N194-03 PYROGENT® 250 test kit 0 .03 . . . . . . . . . . . . . . . . . . . . 326
DE14-701E FBS USDA 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 297 N194-06 PYROGENT® 250 test kit 0 .06 . . . . . . . . . . . . . . . . . . . . 326
DE14-701F FBS USDA 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 297 N194-125 PYROGENT® 250 test kit 0 .125 . . . . . . . . . . . . . . . . . . . 326
DE14-801E FBS EU Standard 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 297 N201 Pyrogen-free Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . 330
DE14-801F FBS EU Standard 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 297 N205 Pyrogen-free Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . 330
DE14-802E FBS EU Standard, Research Grade, 100 ml . . . . . . . . . 297 N207 Pyrogen-free Test Tubes . . . . . . . . . . . . . . . . . . . . . . . . . 330
DE14-802F FBS EU Standard, Research Grade, 500 ml . . . . . . . . 297 N283-06 PYROGENT® Plus 64 tests with CSE 0 .06 . . . . . . . . . . 326
DE14-810E FBS EU Standard (Dialyzed) 100 ml . . . . . . . . . . . . . . 297 N283-125 PYROGENT® Plus 64 tests 0 .125 . . . . . . . . . . . . . . . . . . 326
DE14-810F FBS EU Standard (Dialyzed) 500 ml . . . . . . . . . . . . . . 297 N284-25 PYROGENT® Plus 200 tests 0 .25 . . . . . . . . . . . . . . . . . . 326
DE14-820E FBS EU Standard (charcoal) 100 ml . . . . . . . . . . . . . . 297 N289-06 PYROGENT® Plus single-test with CSE 0 .06 . . . . . . . . 326
DE14-820F FBS EU Standard (charcoal) 500 ml . . . . . . . . . . . . . . 297 N289-125 PYROGENT® Plus single-test with CSE 0 .125 . . . . . . . 326
DE14-830E FBS EU Std (tetracycline free) 100 ml . . . . . . . . . . . . 297 N289-25 PYROGENT® Plus single-test with CSE 0 .25 . . . . . . . . 326
DE14-830F FBS EU Std (tetracycline free) 500 ml . . . . . . . . . . . . 297 N294-03 PYROGENT® Plus 200 tests with CSE 0 .03 . . . . . . . . . 326
DE14-840E FBS EU Standard 100 ml . . . . . . . . . . . . . . . . . . . . . . . . . 296 N294-06 PYROGENT® Plus 200 tests with CSE 0 .06 . . . . . . . . . 326
DE14-840F FBS EU Standard 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . 296 N294-125 PYROGENT® Plus 200 tests 0 .125 . . . . . . . . . . . . . . . . . 326
DE14-850E FBS EU Standard ES 100 ml . . . . . . . . . . . . . . . . . . . . . . 296 N594-03 PYROGENT® Ultra Gel Clot 0 .03 . . . . . . . . . . . . . . . . . . . 327
DE14-850F FBS EU Standard ES 500 ml . . . . . . . . . . . . . . . . . . . . . . 296 N594-06 PYROGENT® Ultra Gel Clot 0 .06 . . . . . . . . . . . . . . . . . . . 327
DE14-860F FBS EU Standard Ultralow IgG 500 ml . . . . . . . . . . . . . 297 N594-125 PYROGENT® Ultra Gel Clot 0 .125 . . . . . . . . . . . . . . . . . . 327
DE14-870E FBS, EU Standard, low IgG 100 ml . . . . . . . . . . . . . . . . . 296 PA-1000 OsteoAssay™ Bone Plate . . . . . . . . . . . . . . . . . . . . . . . . . 237
DE14-870F FBS, EU Standard, low IgG 500 ml . . . . . . . . . . . . . . . . . 296 PA-1500 OsteoLyse™ Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . 238
E50-640 Endotoxin for QCL-1000® . . . . . . . . . . . . . . . . . . . . . . . . . 328 PA-1503 OsteoImage™ Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 240
LT07-111 ViaLight® HS 1,000 tests . . . . . . . . . . . . . . . . . . . . . . . . 223 PA-3010 Phalloidin AlexaFluor®150 U . . . . . . . . . . . . . . . . . . . . . 242
LT07-115 ApoGlow® Screening Kit . . . . . . . . . . . . . . . . . . . . . . . . . 228 PA-3011 Cell Tracker™ Green 250 µg . . . . . . . . . . . . . . . . . . . . . . . 242
LT07-117 ToxiLight® Assay Kit 1,000 tests . . . . . . . . . . . . . . . . . 226 PA-3012 Cell Tracker™ Orange 250 µg . . . . . . . . . . . . . . . . . . . . . . 242
LT07-118 MycoAlert® Kit 10 Tests . . . . . . . . . . . . . . . . . . . . . . . . . . 220 PA-3013 DAPI 10 mg . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 243
LT07-121 ViaLight® Plus 1,000 tests . . . . . . . . . . . . . . . . . . . . . . . 223 PA-3014 Hoechst 33342 Dye 10 ml . . . . . . . . . . . . . . . . . . . . . . . 243
LT07-122 ViaLight® Plus MDA 1,000 tests . . . . . . . . . . . . . . . . . . 223
Indices
PA-3015 LysoTracker® Red 5 × 50 µl . . . . . . . . . . . . . . . . . . . . . . 243

LT07-211 ViaLight® HS 500 tests . . . . . . . . . . . . . . . . . . . . . . . . . . 223 PA-3016 Live/Dead® Viability Assay . . . . . . . . . . . . . . . . . . . . . . . 243
LT07-217 ToxiLight® Assay Kit 500 tests . . . . . . . . . . . . . . . . . . . 226 PA-3017 MitoTracker® Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242
LT07-218 MycoAlert® Kit 25 Tests . . . . . . . . . . . . . . . . . . . . . . . . . . 220 PA-3018 SYTO® 11 Green 250 µl . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
LT07-221 ViaLight® Plus 500 tests . . . . . . . . . . . . . . . . . . . . . . . . . 223 PA-3019 SYTOX® Green 250 µl . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
LT07-311 ViaLight® HS 10,000 tests . . . . . . . . . . . . . . . . . . . . . . . 223 PA-3020 Vybrant® DiI 1 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
LT07-318 MycoAlert® Kit 100 Tests . . . . . . . . . . . . . . . . . . . . . . . . 220 PA-3021 Annexin V, AlexaFluor® 488 250 µl . . . . . . . . . . . . . . . . 244
LT07-321 ViaLight® Plus 10,000 tests . . . . . . . . . . . . . . . . . . . . . . 223 PA-3267 PrimeFect™ 1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
LT07-322 ViaLight® Plus MDA 10,000 tests . . . . . . . . . . . . . . . . . 223 PA-3268 PrimeFect™ 2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203
355
Numerical Index
Continued
PA-3269 PrimeFect™ siRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 PX-2591RL h NHEPS® Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
PA-3271 PrimeFect™ Diluent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 PX-2599RL NHNP Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
PA-4490 CalciFluor™ Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . 239 R-AsM-530 Rat Brain Cx-Hi-Cp Mix cryo amp . . . . . . . . . . . . . . . . . . . 75
PA-6001RL bAEC Cell Pellet, single donor . . . . . . . . . . . . . . . . . . . . . . 70 R-Cb-503 Rat Cerebellar Neurons cyro amp . . . . . . . . . . . . . . . . . . .74
PA-6002RL bAEC Cell Pellet, pooled . . . . . . . . . . . . . . . . . . . . . . . . . . . 70 R-CM-561 Rat Cardiac Myocytes cryo amp . . . . . . . . . . . . . . . . . . . .72
PA-6004RL bPAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 R-Cp-502 Rat Brain Striatum Neuron cryo amp . . . . . . . . . . . . . . . .74
PA-6005RL bCAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 R-CpAs-522 Rat Brain Striatum Astro cryo amp . . . . . . . . . . . . . . . . 75
PT-2501 Mesenchymal Stem Cells cryo amp . . . . . . . . . . . . . . . . 90 R-Cx-500 Rat Brain Cortex Neurons cryo amp . . . . . . . . . . . . . . . . .74
PT-2599 NHMP cryo amp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 R-CxAs-520 Rat Brain Cortex Astro . cryo amp . . . . . . . . . . . . . . . . . . 75
PT-3001 MSCBM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90 R-Drg-505 Rat Dorsal Root Ganglion cryo amp . . . . . . . . . . . . . . . . .74
PT-3002 hMSC Osteogenic BulletKit® . . . . . . . . . . . . . . . . . . . . . . . 90 R-Hi-501 Rat Brain HC Neurons cryo amp . . . . . . . . . . . . . . . . . . . .74
PT-3003 hMSC Chondro BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . 90 R-HiAs-521 Rat Brain HC Astrocytes cryo amp . . . . . . . . . . . . . . . . . 75
PT-3004 hMSC Adipogenic BulletKit® . . . . . . . . . . . . . . . . . . . . . . . 90 S50-641 10 mM MgCl2 Solution 10 ml . . . . . . . . . . . . . . . . . . . . . 330
PT-3238 MSCBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . 90 S50-642 50 mM Tris Buffer 30 ml . . . . . . . . . . . . . . . . . . . . . . . . . 330
PT-3926 HPGM™ 500 ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 SBA-1001 96-well Shuttle® Automation Package . . . . . . . . . . . . 135
PT-4105 MSCGM™ hMSC SingleQuots® Kit . . . . . . . . . . . . . . . . . . . 90 T100A Retronectin® Rec 0 .5 mg . . . . . . . . . . . . . . . . . . . . .78, 204
PT-4124 rhTGF-b3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90 T100B Retronectin® Rec 2 .5 mg . . . . . . . . . . . . . . . . . . . . .78, 204
PT-5001 Pre-adipocytes, sub cutan . . . . . . . . . . . . . . . . . . . . . . . . 91 T110A Retronectin® Rec 35 mm dishes . . . . . . . . . . . . . .78, 204
PT-5005 Pre-adipocytes, visceral . . . . . . . . . . . . . . . . . . . . . . . . . . 91 VBA-1001 HiFect™ Kit, 1 x 825 µl . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
PT-5020 Pre-adipocytes, sub cutan . . . . . . . . . . . . . . . . . . . . . . . . 91 VBA-4001 HiFect™ Kit, 4 x 825 µl . . . . . . . . . . . . . . . . . . . . . . . . . . . 191
PT-7009 AdipoRed™ Assay Reagent . . . . . . . . . . . . . . . . . . . .91, 236 VCA-1001 Cell Line Nucleofector® Kit R . . . . . . . . . . . . . . . . . . . . . 185
PT-8001 OCP BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92 VCA-1002 Cell Line Nucleofector® Kit T . . . . . . . . . . . . . . . . . . . . . . 185
PT-8002 PGM™-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 VCA-1003 Cell Line Nucleofector® Kit V . . . . . . . . . . . . . . . . . . . . . 185
PT-8201 OCP Osteoclast Precur . Basal Medium 100 ml . . . . . . 92 VCA-1004 Cell Line Nucleofector® Kit C . . . . . . . . . . . . . . . . . . . . . 185
PT-8202 PGM™-2 Basal Medium, 500 ml . . . . . . . . . . . . . . . . . . . . 91 VCA-1005 Cell Line Nucleofector® Kit L . . . . . . . . . . . . . . . . . . . . . 185
PT-9501 OCP Osteoclast Precur . SingleQuots® Kit . . . . . . . . . . . 92 VCO-1001 Cell Line Optimization . . . . . . . . . . . . . . . . . . . . . . . . . . . 186
PT-9502 PGM™-2 SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . 91 Nucleofector® Kit, 18 reactions
PX-2501RL NHEK-Ad Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41 VDC-1040 pmax Cloning™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 197
PX-2503RL NHEK-Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42 VDF-1011 pmaxFPTM-Green-C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194
PX-2505RL HMVEC-d Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . 29 VDF-1012 pmaxFPTM-Green-N . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194
PX-2507RL NHEK-Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43 VDF-1013 pmaxFPTM-Green-PRL, . . . . . . . . . . . . . . . . . . . . . . . . . . 194
PX-2508RL PrSC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 VDF-1021 pmaxFPTM-Yellow-C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
PX-2509RL NHDF-Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 VDF-1022 pmaxFPTM-Yellow-N . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
PX-2511RL NHDF-Ad Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38 VDF-1023 pmaxFPTM-Yellow-PRL . . . . . . . . . . . . . . . . . . . . . . . . . . . 195
PX-2512RL NHLF Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39 VDF-1031 pmaxFPTM-Red-C . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
PX-2516RL HMVEC-d Neo Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . 31 VDF-1032 pmaxFPTM-Red-N . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
PX-2517RL HUVEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 VDF-1033 pmaxFPTM-Red-PRL . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
PX-2519RL HUVEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 VFP-1001 Peptide Transfection Control . . . . . . . . . . . . . . . . . . . . . 201
PX-2520RL HUAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33 VGA-1001 cGMPCell Line Nucleofector® Kit R . . . . . . . . . . . . . . . . 188
PX-2527RL HMVEC-L Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 VGA-1002 cGMPCell Line Nucleofector® Kit T . . . . . . . . . . . . . . . . 188
PX-2530RL HPAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32 VGA-1003 cGMPCell Line Nucleofector® Kit V . . . . . . . . . . . . . . . . 188
PX-2535RL HAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 VGA-1004 cGMPCell Line Nucleofector® Kit C . . . . . . . . . . . . . . . . 188
PX-2538RL NHOst Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 VGA-1005 cGMPCell Line Nucleofector® Kit L . . . . . . . . . . . . . . . . 188
PX-2540RL NHBE Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 VHCA-1001 Cell Line 96-well Nucleofector® Kit SE, 96 rxns . . . . 185
PX-2541RL NHBE Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20 VHCA-1002 Cell Line 96-well Nucleofector® Kit SF, 96 rxns . . . . . 185
PX-2543RL HMVEC-d Ad Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28 VHCA-1003 Cell Line 96-well Nucleofector® Kit SG, 96 rxns . . . . 185
PX-2545RL HIAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 VHCA-2001 Cell Line 96-well Nucleofector® Kit SE, 960 rxns . . . 185
PX-2547RL SAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19 VHCA-2002 Cell Line 96-well Nucleofector® Kit SF, 960 rxns . . . . 185
PX-2550RL NHAC-kn Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59 VHCA-2003 Cell Line 96-well Nucleofector® Kit SG, 960 rxns . . . . 185
PX-2551RL HMEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48 VHCA-4001 HeLaS3 96-well Automation Nucleofector® Kit . . . . . 135
PX-2553RL RPTEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 VHCA-4003 Neuro2a 96-well Automation Nucleofector® Kit . . . . 135
PX-2554RL HRCE Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 VHCA-4004 HeLa 96-well Automation Nucleofector® Kit . . . . . . . 135
PX-2555RL PrEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 VHCO-1001 Cell Line Optimization 96-well . . . . . . . . . . . . . . . . . . . 187
PX-2556RL HRE Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55 Nucleofector® Kit, 96 reactions
PX-2559RL NHMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 VHPA-1001 Human B Cell 96 reactions . . . . . . . . . . . . . . . . . . . . . . . 140
PX-2561RL SkMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 VHPA-1002 Human T Cell 96 reactions . . . . . . . . . . . . . . . . . . . . . . . 149
PX-2562RL UtSMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Indices
VHPA-1006 Mouse T Cell 96 reactions . . . . . . . . . . . . . . . . . . . . . . . . 150

PX-2564RL UtMVEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34 VHPA-1007 Human Monocyte 96 reactions . . . . . . . . . . . . . . . . . . 147
PX-2565RL NHA Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50 VHPA-1008 Human Macrophage 96 reactions . . . . . . . . . . . . . . . . 144
PX-2571RL AoSMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 VHPA-1010 Mouse B Cell 96 reactions . . . . . . . . . . . . . . . . . . . . . . . 141
PX-2576RL BSMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 VHPA-1011 Mouse Dendritic Cell (Imm .) 96 reactions . . . . . . . . . 143
PX-2579RL UASMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68 VHPA-1012 Mouse Dendritic Cell (Mat .)96 reactions . . . . . . . . . . 143
PX-2581RL PASMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 VHPA-2001 Human B Cell 960 reactions . . . . . . . . . . . . . . . . . . . . . 140
PX-2583RL CASMC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66 VHPA-2002 Human T Cell 960 reactions . . . . . . . . . . . . . . . . . . . . . 149
PX-2585RL HCAEC Cell Pellet . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 VHPA-2006 Mouse T Cell 960 reactions . . . . . . . . . . . . . . . . . . . . . . 150
356
Numerical Index
Continued
VHPA-2007 Human Monocyte 960 reactions . . . . . . . . . . . . . . . . . 147 VPA-1003 Human CD34+ 25 reactions . . . . . . . . . . . . . . . . . .142, 176
VHPA-2008 Human Macrophage 960 reactions . . . . . . . . . . . . . . . 144 VPA-1004 Human Dendritic 25 reactions . . . . . . . . . . . . . . . . . . . 142
VHPA-2010 Mouse B Cell 960 reactions . . . . . . . . . . . . . . . . . . . . . . 141 VPA-1005 Human NK Cell 25 reactions . . . . . . . . . . . . . . . . . . . . . 148
VHPA-2011 Mouse Dendritic Cell (Imm .) 960 reactions . . . . . . . 143 VPA-1006 Mouse T Cell 25 reactions . . . . . . . . . . . . . . . . . . . . . . . 150
VHPA-2012 Mouse Dendritic Cell (Mat .) 960 reactions . . . . . . . . 143 VPA-1007 Human Monocyte 25 reactions . . . . . . . . . . . . . . . . . . . 146
VHPB-1002 HUVEC 96-well 96 reactions . . . . . . . . . . . . . . . . . . . . . 157 VPA-1008 Human Macrophage 25 reactions . . . . . . . . . . . . . . . . 144
VHPB-2002 HUVEC 96-well 960 reactions . . . . . . . . . . . . . . . . . . . . 157 VPA-1009 Mouse Macrophage 25 reactions . . . . . . . . . . . . . . . . . 145
VHPD-1001 Human Dermal Fibroblast 96 reactions . . . . . . . . . . . 151 VPA-1010 Mouse B Cell 25 reactions . . . . . . . . . . . . . . . . . . . . . . . 141
VHPD-2001 Human Dermal Fibroblast 960 reactions . . . . . . . . . . 151 VPA-1011 Mouse Dendritic (Imm .), 25 reactions . . . . . . . . . . . . 143
VHPF-1001 Human Chondrocyte 96 reactions . . . . . . . . . . . . . . . . 181 VPA-1012 Mouse Dendritic (Mat .), 25 reaction . . . . . . . . . . . . . . 143
VHPF-2001 Human Chondrocyte 960 reactions . . . . . . . . . . . . . . 181 VPB-1001 Human Coronary Artery Endothelial Cell . . . . . . . . . . 156
VHPG-1003 Rat Neuron 96 reactions . . . . . . . . . . . . . . . . . . . . . . . . . 168 25 reactions
VHPG-2003 Rat Neuron 960 reactions . . . . . . . . . . . . . . . . . . . . . . . 168 VPB-1002 Human Umbilical Vein Endothelial Cell 25 reactions 157
VHPH-1001 Mouse Embryonic Stem 96 reactions . . . . . . . . . . . . . 177 VPB-1003 Human Microvascular Endothelial Lung Cell
VHPH-2001 Mouse Embryonic Stem 960 reactions . . . . . . . . . . . . 177 25 reactions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
VHPH-5002 Basic Starter Kit for Human Stem Cells . . . . . . . . . . . . 175 VPC-1001 Human Aortic Smooth Muscle Cell 25 reactions . . . 172
VHPH-5003 Human Stem Cell (H9) Kit . . . . . . . . . . . . . . . . . . . . . . . 176 VPD-1001 Human Dermal Fibroblast 25 reactions . . . . . . . . . . . 151
VHPH-5222 Basic Nucleofector® Kit 2 . . . . . . . . . . . . . . . . . . . . . . . . 175 VPD-1002 Human Keratinocyte 25 reactions . . . . . . . . . . . . . . . 152
for Human Stem Cells, 960 reactions VPD-1003 Human Neonatal Melanocytes (NHEM-Neo) . . . . . . 153
VHPH-5012 Basic Nucleofector® Kit 1 . . . . . . . . . . . . . . . . . . . . . . . . 175 25 reactions
for Human Stem Cells, 96 reactions VPD-1004 Mouse Embryonic Fibroblast . . . . . . . . . . . . . . . . . . . . 182
VHPH-5203 Human Stem Cell (H9) Kit . . . . . . . . . . . . . . . . . . . . . . . 176 Nucleofector® Kit 1, 25 reactions
VHPH-5212 Basic Nucleofector® Kit 1 for Human Stem Cells . . . 175 VPD-1005 Mouse Embryonic Fibroblast . . . . . . . . . . . . . . . . . . . . 182
960 reactions Nucleofector® Kit 2, 25 reactions
VHPH-5022 Basic Nucleofector® Kit 2 for Human Stem Cells . . . 175 VPD-1006 Mouse Embryonic Fibroblasts Starter 10 reactions 182
VHPI-1002 Basic Starter Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 155 VPE-1001 Human Mesenchymal Stem Cell 25 reactions . . . . . 178
for Primary Mammalian Fibroblasts, 64 reactions VPE-1002 Rat Cardiomyocyte Neonatal 25 reactions . . . . . . . . 180
VHPI-1003 Basic Kit for Primary Mammalian Neurons, . . . . . . . 170 VPF-1001 Human Chondrocyte 25 reactions . . . . . . . . . . . . . . . . 181
96 reactions VPG-1001 Mouse Neuron 25 reactions . . . . . . . . . . . . . . . . . . . . . . 167
VHPI-1004 Basic Kit for Primary Smooth Muscle Cells, . . . . . . . . 173 VPG-1002 Chicken Neuron 25 reactions . . . . . . . . . . . . . . . . . . . . 167
96 reactions VPG-1003 Rat Neuron 25 reactions . . . . . . . . . . . . . . . . . . . . . . . . . 168
VHPI-1005 Basic Nucleofector® for Primary . . . . . . . . . . . . . . . . . . 163 VPG-1004 Mouse Neural Stem Cell 25 reactions . . . . . . . . . . . . . 179
Mammalian Epithelial Cells, 64 reactions VPG-1005 Rat Neural Stem Cell 25 reactions . . . . . . . . . . . . . . . . 179
VHPI-1012 Basic Kit 1 for Primary Mammalian Fibroblasts, . . . 155 VPG-1006 Mouse Astrocyte 25 reactions . . . . . . . . . . . . . . . . . . . 166
96 reactions VPG-1007 Rat Astrocyte 25 reactions . . . . . . . . . . . . . . . . . . . . . . . 166
VHPI-1015 Basic Nucleofector® Kit 1 for Primary . . . . . . . . . . . . 163 VPG-1009 Rat Oligodendrocyte 25 reactions . . . . . . . . . . . . . . . . 169
MammalianEpithelial Cells, 96 reactions VPH-1001 Mouse Embryonic Stem Cell 25 reactions . . . . . . . . . 177
VHPI-1022 Basic for Primary Mammalian Fibroblasts, . . . . . . . . 155 VPH-5002 Human Stem Cell Starter 18 reactions . . . . . . . . . . . . 174
96 reactions VPH-5012 Human Stem Cell Kit 1, 25 reactions . . . . . . . . . . . . . . 174
VHPI-1025 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . 163 VPH-5022 Human Stem Cell Kit 2, 25 reactions . . . . . . . . . . . . . . 174
Mammalian Epithelial Cells, 96 reactions VPI-1001 Basic Kit for Primary Mammalian Endothelial Cells, 158
VHPI-2003 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . 170 25 reactions
Mammalian Neurons, 960 reactions VPI-1002 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . . 154
VHPI-2004 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . 173 Fibroblasts, 25 reactions
Smooth Muscle Cells, 960 reactions VPI-1003 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . . 169
VHPI-2012 Basic Nucleofector® Kit 2 for Primary . . . . . . . . . . . . 155 Neural Cells, 25 reactions
Mammalian Fibroblasts, 960 reactions VPI-1004 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . 173
VHPI-2015 Basic Nucleofector® Kit 1 for Primary . . . . . . . . . . . . 163 Smooth Muscle Cells, 25 reactions
Mammalian Epithelial Cells, 960 reactions VPI-1005 Basic Nucleofector® Kit for Primary . . . . . . . . . . . . . . . 163
VHPI-2022 Basic for Primary Mammalian Fibroblasts, . . . . . . . . 155 Mammalian Epithelial Cells, 25 reactions
960 reactions VPK-1001 Normal Human Bronchial Epithelial 25 reactions . . 159
VHPI-2025 Basic for Primary Epithelial Cells, 960 reactions . . . 163 VPK-1002 Human Mammary Epithelial Cell 25 reactions . . . . . 160
VHPK-1001 Human Bronchial Epithelial 96 reactions . . . . . . . . . . 159 VPK-1003 Human Prostate Epithelial Cell 25 reactions . . . . . . . 162
VHPK-1002 Human Mammary Epithelial Cell 96 reactions . . . . . 161 VPL-1002 Mouse Hepatocyte 25 reactions . . . . . . . . . . . . . . . . . . 164
VHPK-1003 Human Prostate Epithelial Cell 96 reactions . . . . . . . 162 VPL-1003 Rat Hepatocyte 25 reactions . . . . . . . . . . . . . . . . . . . . . 165
VHPK-2001 Human Bronchial Epithelial 960 reactions . . . . . . . . 159 VSC-1001 siRNA Test Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200
VHPK-2002 Human Mammary Epithelial Cell 960 reactions . . . . 161 VSPI-1003 Basic Neuron SCN 25 reactions . . . . . . . . . . . . . . . . . . .171
Indices
VHPK-2003 Human Prostate Epithelial Cell 960 reactions . . . . . . 162 VZB-1003 Human Monocyte Nucleofector® Medium, . . . .146, 147
VHPL-1001 Human Hepatocyte 96 reactions . . . . . . . . . . . . . . . . . 164 500 ml
VHPL-1002 Human Hepatocyte 960 reactions . . . . . . . . . . . . . . . . 164 VZB-4003 Human Monocyte Nucleofector® Medium, . . . .146, 147
VMI-1001 Basic Parasite Starter Nucleofector® Kit . . . . . . . . . . . 188 4 x 500 ml
VMI-1011 Basic Parasite Starter Nucleofector® Kit 1 . . . . . . . . . 188 W50-100 LAL Reagent Water 100 ml . . . . . . . . . . . . . . . . . . . . . . . 330
VMI-1021 Basic Parasite Starter Nucleofector® Kit 2 . . . . . . . . . 188 W50-500 LAL Reagent Water 500 ml . . . . . . . . . . . . . . . . . . . . . . . 330
VPA-1001 Human B Cell Nucleofector® Kit . . . . . . . . . . . . . . . . . . 140 W50-640 LAL Reagent Water 30 ml . . . . . . . . . . . . . . . . . . . . . . . . 330
VPA-1002 Human T Cell 25 reactions . . . . . . . . . . . . . . . . . . . . . . . 149
357
Alphabetical Index
96-well Shuttle® Automation Package . . . . . . . . . . . . . . . . . . . . . . . . .135 - 137 L-15 (Leibovitz) Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
HeLaS3 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . . . . . .135 McCoy’s 5A Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
Neuro2a 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . . . . 135 Minimum Essential Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256 - 257
HeLa 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . . . . . . . .135 Medium 199 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
NCTC-109 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
■ A Richter’s CM Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
RPMI 1640 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
ACK Lysing Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .286, 236 Schneiders' Drosophila Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
AdipoRed™ Assay Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91, 236 TC 100 Insect Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
Adult Human Dermal Blood Microvascular Endothelial Cells . . . . . . . . . . .29 Waymouth's MB 752/1 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
Adult Human Dermal Lymphatic Microvascular Endothelial Cells . . . . . .28 Williams' Medium E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
Adult Human Dermal Microvascular Endothelial Cells . . . . . . . . . . . . . . . . .28 Bladder Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21 - 22
Adult Normal Human Epidermal Keratinocytes . . . . . . . . . . . . . . . . . . . . . . . 41 BdSMC – Human Bladder Smooth Muscle Cells . . . . . . . . . . . . . . . 22, 65
Airway Epithelial Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18 - 20 Bladder Microvascular Endothelial Cell Growth Media . . . . . . . . . . . . . .22
BEGM® Bronchial Epithelial Cell Growth Media . . . . . . . . . . . . . . . . . 19, 20 Bladder Smooth Muscle Cell Growth Media . . . . . . . . . . . . . . . . . . . . . . .22
NHBE – Normal Human Bronchial/Tracheal Epithelial Cells . . . . . . . . . 19 HMVEC-Bd – Human Bladder Microvascular Endothelial Cells . . . . . .22
SAEC – Human Small Airway Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . 19 Bladder Microvascular Endothelial Cell Growth Media . . . . . . . . . . . . . . . . .22
SAGM™ Small Airway Epithelial Cell Growth Media . . . . . . . . . . . . . 19, 20 Bladder Smooth Muscle Cell Growth Media . . . . . . . . . . . . . . . . . . . . . . . 22, 65
Amaxa® Nucleofection® and Transfection . . . . . . . . . . . . . . . . . . . . . .126 - 127 SmBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65
Amaxa® Nucleofector® Technology . . . . . . . . . . . . . . . . . . . . . . . . . . . .128 - 131 SmGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65
Amniochrome® II Modified Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273 SmGM®-2 SingleQuots® Supplements and Growth Factors . . . . . 22, 65
Amniochrome® Plus Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273 Blood Brain Barrier Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Amniochrome® Pro Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273 bMVEC-B – Bovine Brain Microvascular Endothelial Cells . . . . . . . . . . . . . . 13
Amphotericin B . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .199, 285 Bone Marrow and Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 - 85
Animal and Human Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289 - 297 Human Cord Blood Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .84
Handling Serum Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 292 Human Bone Marrow Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . .84
Inactivation of Viruses and Mycoplasma by Gamma Irradiation . . 292 Normal Human Peripheral Blood Leukopaks . . . . . . . . . . . . . . . . . . . . . .83
Origin and Types of Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .291 Quadratox 4 Species Panel of Bone Marrow Mononuclear Cells . . . . .85
Quality Assurance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 290 Unprocessed Human Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83
Serum Tests for Special Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . .291 Bone Marrow CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Special Handling Fees . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 Bone Marrow Non-Irradiated Stromal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . .89
Special Serum Treatments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .293 Bovine Aortic Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70
Animal Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 - 77 Bovine Aortic Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . . . . . . . .70
Annexin V, Alexa Fluor® 488 Conjugate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244 Bovine Brain Extract (BBE) 9 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Antibiotics and Antimycotics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .199, 285 Bovine Brain Microvascular Endothelial Cell Growth Media . . . . . . . . . . . . 13
AoAF – Human Aortic Adventitial Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . 37 Bovine Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
AoSMC – Human Aortic Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . 65 Bovine Pituitary Extract (BPE) 13 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
ApoGlow® Rapid Apoptosis Screening . . . . . . . . . . . . . . . . . . . . . . . . . 228 - 229 Bovine Pulmonary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Ascorbic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60 Bovine Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
Ascorbic Acid 25 .5 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
ATP Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 Calf Serum, Newborn . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
bPAEC – Bovine Pulmonary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . 71
■ B Bronchial Epithelial Cell Growth Media . . . . . . . . . . . . . . . . . . . . . . . . . . . 19, 20
BSMC – Normal Human Bronchial Smooth Muscle Cells . . . . . . . . . . . . . . .66
b-G-Blocker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330 Buffers and Buffered Salines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 - 287
bAEC – Bovine Aortic Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . . . . .70
bAEC – Bovine Aortic Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . .70 ■ C
Basal Medium Eagle (BME) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253
Basic Parasite Nucleofector® Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .188 Calcium Chloride Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
bCAEC – Bovine Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . 71 CalciFluor™ Quantitative Bone Resorption Assay . . . . . . . . . . . . . . . . . . . . .239
BdSMC – Human Bladder Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . 22, 65 Calcium Chloride 300 mM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
BEGM® Bronchial Epithelial Cell Growth Media . . . . . . . . . . . . . . . . . . . . 19, 20 Calf Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
BioAssay Accessory Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .241 CASMC – Human Coronary Artery Smooth Muscle Cells . . . . . . . . . . . . . . .66
BioAssays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 217 - 219 CD14+ Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93
BioAssays FAQs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 247 - 248 CD34+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86
Bioservices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 246 CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
Cell Analysis Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242 - 244 Cell Culture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 281 - 287
G Protein-Coupled Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .245 Balanced Salt Solutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282
Indices
BioWhittaker® Classical Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 251 - 259 Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283

Basal Medium Eagle (BME) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .253 Cell Culture Technical Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 298
Dulbecco's MEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253 - 254 Adaptation of Cell Cultures to Serum-free Medium . . . . . . . . . . . . . . 298
DMEM:F12 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 Cell Culture FAQs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 306 -308
Glasgow Minimum Essential Medium (GMEM) . . . . . . . . . . . . . . . . . . .255 Cell Culture Reagent Formulations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .321
Grace’s Insect Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 Cryopreservation and Reconstitution . . . . . . . . . . . . . . . . . . . . . . . . . . 300
Ham’s Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255 Determination of Cell Numbers . . . . . . . . . . . . . . . . . . . . . . . . . . . 301 - 302
Iscove’s Modified Dulbecco’s Medium (IMDM) . . . . . . . . . . . . . . . . . . .255 Formulations for BioWhittaker® Classical Media . . . . . . . . . . . . 309 - 320
358
Alphabetical Index
Continued
Powdered Media Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 303 ■ E

Protocols for Weaning Cell Cultures . . . . . . . . . . . . . . . . . . . . . . . . . . . . 299
Subculturing Procedures for Mammalian Cells . . . . . . . . . . . . . 304 - 305 Earle's BSS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282
CellTracker™ Green Fluorescent Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242 EBM®-2 Endothelial Cell Basal Medium-2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
CellTracker™ Orange Fluorescent Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242 EGM® (Endothelial Growth Medium) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
cGMP Cell Line Nucleofector® Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .188 ELx808LBS Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
Chondrocyte ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . .60 EBM®-2 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 35
Clear Bottom, White Walled Tissue Culture Plates . . . . . . . . . . . . . . . . . . . .241 EBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 35
Clonetics® Cell Models . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 - 13 EGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22, 25, 32 - 35
Blood Brain Barrier Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 EGM®-2MV BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 25 - 31, 34, 35
bMVEC-B – Bovine Brain Microvascular Endothelial Cells . . . . . . . . . . 13 EGM®-2MV SingleQuots® Supplements and Growth Factors . . . . . . . . 22, 35
Bovine Brain Microvascular Endothelial Cell Growth Media . . . . . . . . . 13 EGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . . . .35
Corneal Epithelial Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 EGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33, 34, 35
Corneal Epithelial Model BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 EGM® Complete Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
EMVB BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 EGM®-2MV SingleQuots® Supplements and Growth Factors . . . . . . . . 22, 35
Renal Epithelial Cell Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 EGM®-MV BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70-71
Clonetics® Conditionally Immortalized Cell Lines . . . . . . . . . . . . . . . . . . . . . . 8 EMVB BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Clonetics® FAQs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99 - 100 EMVB SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . . . . . 13
Clonetics® Technical Information: Cells and Media . . . . . . . . . . . . . 101 - 110 Endothelial Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23, 34, 69 - 71
Culture Set Up – Adherent Cell Types . . . . . . . . . . . . . . . . . . . . . . . . . . .104 bAEC – Bovine Aortic Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . .70
Improving Cell Yield and Viability During Subculture . . . . . . . . . . . . . .110 bAEC – Bovine Aortic Endothelial Cells, Single Donor . . . . . . . . . . . . . .70
Instructions for Cryopreservation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .109 bCAEC – Bovine Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . . 71
Media Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102 - 103 bPAEC – Bovine Pulmonary Artery Endothelial Cells . . . . . . . . . . . . . . . 71
Proliferating Cells – Adherent Cell Types . . . . . . . . . . . . . . . . . . . . . . . .105 HAEC – Human Aortic Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . .25
Safety Precautions with Clonetics® Cells . . . . . . . . . . . . . . . . . . . . . . . .102 HCAEC – Human Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . .25
Seeding – Adherent Cell Types . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105 HIAEC – Human Iliac Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . .26
Subculturing – Adherent Cell Types . . . . . . . . . . . . . . . . . . . . . . . 106 - 107 HMVEC-C – Human Cardiac Microvascular Endothelial Cells . . . . . . . . 31
Subculturing into 96-well plates . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108 HMVEC-dAd – Adult Human Dermal Microvascular Endothelial Cells 28
Thawing Cells – Adherent Cell Types . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 HMVEC-dBlAd – Adult Human Dermal Blood
Clonetics® Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 111 - 120 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Airway Cell Media Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .112 HMVEC-dBlNeo – Neonatal Human Dermal Blood
Endothelial Cell Media Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113, 114 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
Fibroblast Cell Media Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115 HMVEC-dLyAd – Adult Human Dermal Lymphatic
Hepatocyte Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .115 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Keratinocyte Cell Media Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .116 HMVEC-dLyNeo – Neonatal Human Dermal Lymphatic
Mammary Epithelial Cell Media, Serum-free . . . . . . . . . . . . . . . . . . . . .117 Microvascular Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . .30
Melanocyte Cell Medium, Serum-free . . . . . . . . . . . . . . . . . . . . . . . . . . .117 HMVEC-dNeo – Neonatal Human Dermal
Neural Cell Medium, Low Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117 Microvascular Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . . . . . . . 31
Primary Neuron Growth Medium, Serum-free . . . . . . . . . . . . . . . . . . . .118 HMVEC-dNeo – Neonatal Human Dermal
Prostate Epithelial Cell Medium, Serum-free . . . . . . . . . . . . . . . . . . . . .118 Microvascular Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . .29
Renal Cell Media, Low Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .118 HMVEC-LBl – Human Lung Blood
Skeletal Cell Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Skeletal Muscle Cell Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .119 HMVEC-L – Human Lung Microvascular Endothelial Cells . . . . . . . . . .26
Smooth Muscle Cell Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 HPAEC – Human Pulmonary Artery Endothelial Cells . . . . . . . . . . . . . .32
Stromal Cell Medium, Low Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .120 HUAEC – Human Umbilical Artery Endothelial Cells . . . . . . . . . . . . . . . .33
Control Standard Endotoxin for Gel Clot LAL . . . . . . . . . . . . . . . . . . . . . . . . .327 HUVEC – Human Umbilical Vein Endothelial Cells, Pooled . . . . . . . . . .34
Cord Blood CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor . . . . .33
Cord Blood Erythroid Progenitors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88 UtMVEC-Myo – Human Uterine Microvascular Endothelial . . . . . . . . .34
Custom Cell Isolation Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .98 Endothelial Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Custom Cell Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 EBM®-2 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 - 35
Custom Cell Isolations and Cell Culture Services . . . . . . . . . . . . . . . . . .79 EBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 35
Human and Animal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 EGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22, 25, 32 - 35
Quality Assurance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 EGM®-2MV BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 25 - 31, 34, 35
Cytogenetics Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273 EGM®-2MV SingleQuots® Supplements and Growth Factors . . . . 22, 35
EGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . .35
■ D EGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33, 34, 35
EGM® Complete Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
DAPI Nucleic Acid Stain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .243 EGM®-MV BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33, 35, 70 -71
Indices
Dextrose-Gelatin-Veronal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 286 EGM®-MV SingleQuots® Supplements and Growth Factors . . . . . . . . . .35

DMEM . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 253 - 254 EGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .35
DMEM:F12 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .254 EMVB BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Donor Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 EMVB SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . . 13
Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Umbilical Cord Blood Program . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Endotoxin for Gel Clot Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .327
DPBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .63, 90, 91 Endotoxin for QCL-1000® Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 328
Endpoint Chromogenic LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 328
359
Alphabetical Index
Continued
QCL-1000® Endpoint Chromogenic LAL Assay . . . . . . . . . . . . . . . . . . . 328 Validation Test Results . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .335
Epidermal Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 Flx 800LBS Fluorescent Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
Epidermal Keratinocyte Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . 40 - 44
NHEK-Ad – Adult Normal Human Epidermal Keratinocytes . . . . . . . . . 41 ■ G
NHEK-Neo – Neonatal Normal Human Epidermal Keratinocytes . . . .42
NHEK-Neo – Neonatal Normal Human Epidermal . . . . . . . . . . . . . . . . .43 G Protein Coupled Receptors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .245
Keratinocytes, Pooled Grace's Insect Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255
Epidermal Keratinocyte Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 Gel Clot LAL Assays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326
KBM®-2 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 PYROGENT® Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326
KBM®-2 w/o Ca++ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 PYROGENT® Plus Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . 326
KBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 PYROGENT® Ultra Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . .327
KBM®-CD Keratinocyte Basal Medium, Chemically Defined . . . . . . . . .44 Gene Expression Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 209 - 216
KBM® Basal Medium w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 Gentamicin Sulfate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285
KGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41, 42, 43, 44 Glasgow Minimum Essential Medium (GMEM) . . . . . . . . . . . . . . . . . . . . . .255
KGM®-2 BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
KGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . .44 ■ H
KGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
KGM® BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 HAEC – Human Aortic Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
KGM®-CD BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 Ham's Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .255
KGM®-CD SingleQuots® Supplements and Growth Factors . . . . . . . . . .44 Hanks' BSS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 282
KGM® Complete System (supplemented) . . . . . . . . . . . . . . . . . . . . . . . .44 HCAEC – Human Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . .25
KGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .44 hEGF 3 µg/ml Epidermal Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44 Hematopoietic Progenitor Cells and Media . . . . . . . . . . . . . . . . . . . . . . .86 - 89
Epidermal Melanocyte Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . .45 - 46 Hepatocyte Culture Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15, 17
NHEM-Neo – Neonatal Normal Human Epidermal Melanocytes . . . .46 Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Epidermal Melanocyte Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 HEPES Buffered Saline Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78, 286
ET-3 Growth Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 hFGF 1 µg/ml Human Fibroblastic Growth Factor . . . . . . . . . . . . . . . . . . . . . 78
MBM®-4 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 HIAEC – Human Iliac Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . .26
MGM®-4 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 HiFect® Transfection Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .191
MGM®-4 SingleQuots® Supplements and Growth Factors . . . . . . . . . . .46 HL-1™ Chemically Defined, Serum-free Medium . . . . . . . . . . . . . . . . 274 - 275
Phosphate Buffered Saline (PBS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 HMEC – Human Mammary Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . .48
Equine Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 hMSC Human Mesenchymal
Horse Serum . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 hMSC Mesenchymal Stem Cell Adipogenic Differentiation BulletKit® . . .90
Erythroid Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .88 hMSC Mesenchymal Stem Cell Chondrogenic
ET-3 Growth Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 Differentiation BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90
ExPro™ Luminometer Injector Cleaning Solution . . . . . . . . . . . . . . . . . . . . .241 hMSC Mesenchymal Stem Cell Osteogenic Differentiation BulletKit® . . .90
HMVEC-Bd – Human Bladder Microvascular Endothelial Cells . . . . . . . . . .22
■ F HMVEC-C – Human Cardiac Microvascular Endothelial Cells . . . . . . . . . . . 31
HMVEC-dAd – Adult Human Dermal Microvascular Endothelial Cells . . . .28
Fetal Bovine Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 HMVEC-dBlAd – Adult Human Dermal Blood
Fetal Bovine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 294 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .29
Fetal Bovine Serum (Available Outside USA and Canada) . . . .296, 297 HMVEC-dBlNeo – Neonatal Human Dermal Blood
Fetal Liver CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
Fibroblast Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .36 - 39 HMVEC-dLyAd – Adult Human Dermal Lymphatic
AoAF – Human Aortic Adventitial Fibroblasts . . . . . . . . . . . . . . . . . . . . . 37 Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
HPdLF – Human Periodontal Ligament Fibroblasts . . . . . . . . . . . . . . . . 37 HMVEC-dLyNeo – Neonatal Human Dermal Lymphatic
NHDF-Ad – Human Adult Dermal Fibroblasts . . . . . . . . . . . . . . . . . . . . . .38 Microvascular Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . .30
NHDF-Neo – Human Neonatal Dermal Fibroblasts . . . . . . . . . . . . . . . . .38 HMVEC-dNeo – Neonatal Human Dermal
NHLF – Human Lung Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Microvascular Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . . . . . . . 31
Fibroblastic Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
HMVEC-dNeo – Neonatal Human Dermal
Fibroblast Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
Microvascular Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . .29
FBM® Fibroblast Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
HMVEC-LBl – Human Lung Blood
FGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38, 39
Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
FGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . .39
HMVEC-L – Human Lung Microvascular Endothelial Cells . . . . . . . . . . . . .26
FGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
HMVEC-LLy – Human Lung Lymphatic Microvascular Endothelial Cells 27
FGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .39
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 h NHEPS® – Human Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Hoechst 33342 Nuclear Counterstain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .243
Indices
SCBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39

SCGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 HPAEC – Human Pulmonary Artery Endothelial Cells . . . . . . . . . . . . . . . . . .32
SCGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . .39 HPdLF – Human Periodontal Ligament Fibroblasts . . . . . . . . . . . . . . . . . . . 37
Flexible Bioprocess Containers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .333 HPGM™ Hematopoietic Progenitor Growth Medium . . . . . . . . . . . . . . . . . . . .87
Bioprocess Tankliners . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .337 HRCE – Human Renal Cortical Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . .55
Platinum UltraPAK™ Advantages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 336 HRE – Human Renal Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
Standard Platinum UltraPAK™ Bag Configurations . . . . . . . . . . . . . . . 336 HSMM – Human Skeletal Muscle Myoblasts . . . . . . . . . . . . . . . . . . . . . . . . . .62
Tubing Sets and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 336 HUAEC – Human Umbilical Artery Endothelial Cells . . . . . . . . . . . . . . . . . . .33
Validation Testing Methods & Results . . . . . . . . . . . . . . . . . . . . . . . . . . 334 Human Adult Dermal Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
360
Alphabetical Index
Continued
Human Aortic Adventitial Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 Human Renal Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55

Human Aortic Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Human Renal Proximal Tubule Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . .56
Human Aortic Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 Human Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
Human Bladder Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . .22 Human Skeletal Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
Human Bladder Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22 Human Skeletal Muscle Myoblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
Human Bone Marrow CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . .87 Human Small Airway Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Human Bone Marrow Non-irradiated Stromal Cells . . . . . . . . . . . . . . . . . . . .89 Human Stromal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .89
Human Cardiac Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . 31 Human T and B Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
Human CD34+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .86 - 87 Human Cord Blood CD4+ T Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
Human Bone Marrow CD34+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . .86 Human Cord Blood CD19+ B Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
Human Cord Blood CD34+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . .87 Human Peripheral Blood CD4+ T Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .95
Human Fetal Liver CD34+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . .87 Human Transferrin 10 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Human CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87 Human Umbilical Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Human Cord Blood CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . .87 Human Umbilical Artery Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . .68
Human Coronary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .25 Human Umbilical Vein Endothelial Cells, Pooled . . . . . . . . . . . . . . . . . . . . . .34
Human Coronary Artery Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . .66 Human Umbilical Vein Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . .33
Human Dendritic Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Human Uterine Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . .34
HPBMC – Human Peripheral Blood Mononuclear Cells . . . . . . . . . . . . .94 Human Visceral Preadipocyte Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
NHDC – Normal Human Dendritic Cells . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Human Subcultureous Preadipocytes Cells . . . . . . . . . . . . . . . . . . . . . . . . . .91
Human Dendritic Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 HUVEC – Human Umbilical Vein Endothelial Cells, Pooled . . . . . . . . . . . . . .34
LGM-3® Lymphocyte Growth Medium-3 . . . . . . . . . . . . . . . . . . . . . . . . . .94 HUVEC – Human Umbilical Vein Endothelial Cells, Single Donor . . . . . . . .33
Human Epidermal Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Human Fetal Liver CD133+ Progenitor Cells . . . . . . . . . . . . . . . . . . . . . . . . . .87 ■ I
Human Fibroblastic Growth Factors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Human Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Insect-XPRESS™ Protein-free Insect Cell Medium . . . . . . . . . . . . . . . . . . . .278
Human Hepatocytes Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 Iscove’s Modified Dulbecco’s Medium (IMDM) . . . . . . . . . . . . . . . . . . . . . . .255
Human Iliac Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .26 ITS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
Human Lung Blood Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . .27 ITES . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
Human Lung Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 IVF Culture Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272
Human Lung Lymphatic Microvascular Endothelial Cells . . . . . . . . . . . . . .27
Human Lung Microvascular Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . .26 ■ K
Human Mammary Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48 KBM®-2 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
Human Mesenchymal Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 KBM®-2 w/o Ca++ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
Human Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 KBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
Human Bone Marrow Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . .84 KBM® Basal Medium w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
Human Cord Blood Mononuclear Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .84 KBM®-CD Keratinocyte Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
Human Natural Killer (NK) Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .96 KGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
Human Neonatal Dermal Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38 KGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41, 42, 43, 44
Human Neural Progenitor Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . .97 KGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . . . .44
Neural Progenitor Supplement SingleQuots® Kit . . . . . . . . . . . . . . . . . .97 KGM®-2 BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
NHNP - Normal Human Neural Progenitor Cells . . . . . . . . . . . . . . . . . . .97 KGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41, 42, 43, 44
NHNP - Cell Pellet in RNALater . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97 KGM® BulletKit® w/o Ca++ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
NPBM Neural Progenitor Basal Medium SingleQuots® Kit . . . . . . . . . .97 KGM®-CD BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
NPDM Neural Progenitor Differentiation Medium BulletKet® . . . . . . . .97 KGM®-CD SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .44
NPMM Neural Progenitor Maintenance Medium BulletKit® . . . . . . . . .97 KGM® Complete System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
NPMM Neural Progenitor maintenance Medium . . . . . . . . . . . . . . . . . . .97 KGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . . . . .44
Human Osteoclast Precursor Cells and Media . . . . . . . . . . . . . . . . . . . . . . . .92
OCP Osteoclast Medium BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92
■ L
OCP Osteoclast Precursor Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . .92
OCP Osteoclast Precursor SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . .92 L-15 (Leibovitz) Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256
Human Periodontal Ligament Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37 L-glutamine . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
Human Peripheral Blood CD14+ Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . .93 LAL Accessory Products . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
Human Preadipocyte Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 LAL Reagent water . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
AdipoRed™ Assay Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 Live/Dead® Viability/Cytotoxicity Assay Kit . . . . . . . . . . . . . . . . . . . . . . . . .243
DPBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 Lymphochrome™ Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272
Human Visceral Preadipocyte Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 LysoTracker® Red Lysosomal Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .243
Human Subcutaneous Preadipocyte Cells . . . . . . . . . . . . . . . . . . . . . . . .91
PGM™-2 Preadipocyte Basal Medium-2 . . . . . . . . . . . . . . . . . . . . . . . . . . .91 ■ M
Indices
PGM™-2 Preadipocyte Growth Medium-2 BulletKit® . . . . . . . . . . . . . . .91

PGM™-2 Preadipocyte Growth Medium SingleQuots® Kit . . . . . . . . . . .91 Mammary Epithelial Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47 - 48
Human Prostate Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 HMEC – Human Mammary Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . .48
Human Prostate Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 Mammary Epithelial Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
Human Prostate Stromal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 MEBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
Human Pulmonary Artery Endothelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . .32 MEBM® Phenol Red-free Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . .48
Human Pulmonary Artery Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . 67 MEBM® Sodium Bicarbonate-free Basal Medium . . . . . . . . . . . . . . . . . .48
Human Renal Cortical Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55 MEGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
361
Alphabetical Index
Continued
MEGM® Complete Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48 Nucleofector® II Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132

MEGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . .48 Nucleofector® Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138 - 182
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48 96-well Shuttle® Automation Package . . . . . . . . . . . . . . . . . . . . .135 - 137
McCoy’s 5A Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256 HeLaS3 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . .135
Medium 199 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 Neuro2a 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . 135
MEM NEAA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283 HeLa 96-well Automation Nucleofector® Kit . . . . . . . . . . . . . . . . .135
MEM Eagle Vitamin Mixture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283 Blood Cell Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
Mesenchymal Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 Human B Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
Mesenchymal Stem Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90 Human B Cell 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .140
MgCl2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330 Human CD34+ Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142
Minimum Essential Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 256 - 257 Human Dendritic Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .142
MitoTracker® Red CMxRos Mitochondrial Probe . . . . . . . . . . . . . . . . . . . . . 242 Human Macrophage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .144
MycoZap™ Mycoplasma Elimination Reagent . . . . . . . . . . . . . . . . . . .198, 222 Human Macrophage 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .144
MycoAlert® Mycoplasma Detection Kit . . . . . . . . . . . . . . . . . . . . . . . 220 - 221 Human Monocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .146
Human Monocyte 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .147
■ N Human Natural Killer Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .148
Human T Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149
NCTC-109 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 Human T Cell 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .149
Neonatal Human Dermal Lymphatic Mouse B Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
Microvascular Endothelial Cells, Single Donor . . . . . . . . . . . . . . . . . . .30 Mouse B Cell 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .141
Neonatal Human Dermal Microvascular Endothelial Cells, Pooled . . . . . . 31 Mouse Dendritic Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .143
Neonatal Human Dermal Microvascular Endothelial Cells, Single Donor 29 Mouse Dendritic Cell 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .143
Neonatal Normal Human Epidermal Keratinocytes . . . . . . . . . . . . . . . . . . .42 Mouse Macrophage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .145
Neonatal Normal Human Epidermal Keratinocytes, Pooled . . . . . . . . . . . .43 Mouse T Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .150
Neonatal Normal Human Epidermal Melanocytes . . . . . . . . . . . . . . . . . . . .46 Mouse T Cell 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .150
Neural Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49 - 50 Dermal Cell Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151 - 155
NHA – Normal Human Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Human Dermal Fibroblast (Basic) . . . . . . . . . . . . . . . . . . . . . . . . .151
Neural Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Human Dermal Fibroblast 96-well (Basic) . . . . . . . . . . . . . . . . . .151
ABM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Human Keratinocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .152
AGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Normal Human Epidermal Melanocyte-Neo . . . . . . . . . . . . . . . . .153
AGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .50 Primary Mammalian Fibroblasts (Basic) . . . . . . . . . . . . . . . . . . .154
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Primary Mammalian Fibroblasts 96-well . . . . . . . . . . . . . . . . . . .155
NHAC-kn – Normal Human Articular Chondrocytes, Knee . . . . . . . . . . . . .59 Endothelial Cell Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . 156 - 158
NHA – Normal Human Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Human Coronary Artery Endothelial Cell . . . . . . . . . . . . . . . . . . . .156
NHBE – Normal Human Bronchial/Tracheal Epithelial Cells . . . . . . . . . . . . 19 Human Microvascular Endothelial Cell-Lung . . . . . . . . . . . . . . . .156
NHDC – Normal Human Dendritic Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Human Umbilical Vein Endothelial Cell . . . . . . . . . . . . . . . . . . . . .157
NHDF-Ad – Human Adult Dermal Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . .38 Human Umbilical Vein Endothelial Cell 96-well . . . . . . . . . . . . . .157
NHDF-Neo – Human Neonatal Dermal Fibroblasts . . . . . . . . . . . . . . . . . . . .38 Human Primary Mammalian Endothelial Cells . . . . . . . . . . . . . . .158
NHEK-Ad – Adult Normal Human Epidermal Keratinocytes . . . . . . . . . . . . 41 Epithelial Cell Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . 159 - 163
NHEK-Neo – Neonatal Normal Human Epidermal Keratinocytes . . . . . . .42 Human Mammary Epithelial Cell . . . . . . . . . . . . . . . . . . . . . . . . . . .160
NHEK-Neo – Neonatal Normal Human Epidermal Keratinocytes, Pooled 43 Human Mammary Epithelial Cell 96-well . . . . . . . . . . . . . . . . . . . . 161
NHEM-Neo – Neonatal Normal Human Epidermal Melanocytes . . . . . . . .46 Human Prostate Epithelial Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . .162
NHLF – Human Lung Fibroblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39 Human Prostate Epithelial Cell 96-well . . . . . . . . . . . . . . . . . . . . .162
NHMC – Normal Human Mesangial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Normal Human Bronchial Epithelial Cell . . . . . . . . . . . . . . . . . . . .159
NHOst – Normal Human Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Normal Human Bronchial Epithelial Cell 96-well . . . . . . . . . . . . .159
Normal Human Articular Chondrocytes, Knee . . . . . . . . . . . . . . . . . . . . . . . .59 Primary Mammalian Epithelial Cells (Basic) . . . . . . . . . . . . . . . .163
Normal Human Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .50 Primary Mammalian Epithelial Cells 96-well (Basic) . . . . . . . . .163
Normal Human Bronchial Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . .66 Hepatocyte Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . 164 - 165
Normal Human Bronchial/Tracheal Epithelial Cells . . . . . . . . . . . . . . . . . . . . 19 Human Hepatocyte 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164
Normal Human Dendritic Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .94 Mouse Hepatocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .164
Normal Human Mesangial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 Rat Hepatocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .165
Normal Human Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .59 Human Chondrocyte Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . .181
Normal Human Peripheral Blood Leukopaks . . . . . . . . . . . . . . . . . . . . . . . . .83 Human Chondrocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .181
NSF-1 Neural Survivor Factor-1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 Human Chondrocyte 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .181
Nucleofection® FAQs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 189 - 190 Human Monocyte Nucleofector® Kits . . . . . . . . . . . . . . . . . . . . . 146 - 147
Nucleofection® of Cell Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185 - 187 Human Monocyte 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .147
Cell Line Optimization Nucleofector® Kit . . . . . . . . . . . . . . . . . . . . . . . . 186 Human Monocyte Nucleofector® Medium . . . . . . . . . . . . . 146 -147
Indices
Cell Line Optimization 96-well Nucleofector® Kit . . . . . . . . . . . . . . . . .187 List of Nucleofector® Kits for Primary Cells . . . . . . . . . . . . . . . . . 138, 139
High Throughput Nucleofection® Nucleofector® Kits of Cell Lines . .185 MEF (Mouse Embryonic Fibroblast) . . . . . . . . . . . . . . . . . . . . . . . . . . . .182
Nucleofection® of Cell Lines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185 - 187 Neural Cell Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166 - 169
Optimized Protocols for Cell Lines . . . . . . . . . . . . . . . . . . . . . . . . 183 - 184 Basic Neuron SCN . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
Nucleofector® Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .132 Chicken Neuron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167
96-well Shuttle® Device . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .133 Mouse Astrocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .166
96-well Shuttle® Device Extended Guarantee and Service . . . . . . . .134 Mouse Neuron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .167
Nucleofector® Device Extended Guarantee and Service . . . . . . . . . . .134 Primary Mammalian Neurons (Basic) . . . . . . . . . . . . . . . . . . . . . .169
362
Alphabetical Index
Continued
Primary Mammalian Neurons 96-well . . . . . . . . . . . . . . . . . . . . . .170 hEGF 3 µg/ml Epidermal Growth Factor . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Astrocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .166 HEPES Buffered Saline Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Neuron . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 hFGF 1 µg/ml Human Fibroblastic Growth Factor . . . . . . . . . . . . . . . . . . 78
Rat Neuron 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .168 Human Transferrin 10 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Oligodendrocyte . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .169 NSF-1 Neural Survivor Factor-1 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Cardiomyocyte Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . 180 ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Rat Cardiomyocyte – Neonatal . . . . . . . . . . . . . . . . . . . . . . . . . . . 180 Retronectin Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Smooth Muscle Cells Nuceofector Kits . . . . . . . . . . . . . . . . . . . . .172 - 173 Retronectin® Recombinant Human Fibronectin Fragment . . . . . . . . . 78
Human Aortic Smooth Muscle Cell (Basic) . . . . . . . . . . . . . . . . . . 172 Trypsin/EDTA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Primary Smooth Muscle Cells (Basic) . . . . . . . . . . . . . . . . . . . . . .173 Trypsin Neutralizing Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
Primary Smooth Muscle Cells 96-well (Basic) . . . . . . . . . . . . . . .173 PrimeFect™ DNA and siRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 - 204
Stem Cell Nucleofector Kits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .174 - 179 Primary Neural Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .73 - 77
Human CD34+ Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .176 Mouse C57 Brain Cortex Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Human MSC (Mesenchymal Stem Cell) . . . . . . . . . . . . . . . . . . . . .178 Mouse C57 Brain Striatum Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Human Stem Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 174 Mouse CD1 Brain Cortex Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Human Stem Cell (H9) 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . .176 Mouse CD1 Brain Mixed Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Human Stem Cells 96-well (Basic) . . . . . . . . . . . . . . . . . . . . . . . . .175 Mouse CD1 Brain Striatum Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Mouse ES Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 Mouse CD57 Brain Mixed Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76
Mouse ES Cell 96-well . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .177 Rat Brain Cortex Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Mouse NSC (Neural Stem Cell) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .179 Rat Brain Cortex Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Rat NSC (Neural Stem Cell) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .179 Rat Brain Cx-Hi-Cp Mix Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Rat Brain Hippocampus Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
■ O Rat Brain Hippocampus Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Rat Brain Striatum Astrocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
OCP Osteoclast Precursor Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Rat Brain Striatum Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Optimized Protocols for Cell Line Transfection . . . . . . . . . . . . . . . . . 183 - 184 Rat Cerebellar Neurons (Granular Cells) . . . . . . . . . . . . . . . . . . . . . . . . . . 74
OsteoAssay™ Human Bone Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .237 Rat Dorsal Root Ganglion Neurons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Osteoclast Precursor Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .92 Primary Neuronal Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .74 - 77
OsteoImage™ Mineralization Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 240 ABM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
OsteoLyse™ Assay Kit (Human Collagen) . . . . . . . . . . . . . . . . . . . . . . . . . . .238 AGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .75 - 77
AGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .77
■ P PNBM™ Primary Neuron Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . .77
PNGM™-A Primary Neuron Growth Medium - Adult BulletKit® . . . 74, 77
PASMC – Human Pulmonary Artery Smooth Muscle Cells . . . . . . . . . . . . . 67 PNGM™-A Primary Neuron Growth
PBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46, 287 Medium - Adult SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
PDELight® HTS cAMP Phosphodiesterase . . . . . . . . . . . . . . . . . . . . . 232 - 233 PNGM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
Penicillin-Streptomycin Mixture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 285 PNGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74, 76 - 77
Penicillin-Streptomycin-Amphotericin B Mixture . . . . . . . . . . . . . . . . . . . . 285 PNGM™ SingleQuots® Supplement and Growth Factor . . . . . . . . . . . . . .77
Penicillin-Streptomycin -L-glutamine Mixture . . . . . . . . . . . . . . . . . . . . . . 285 ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77
Peptide Transfection Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .201 Pro293™ Chemically Defined, Serum-free Media . . . . . . . . . . . . . . . . . . . . .270
Peripheral Blood CD14+ Monocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .93 ProCHO™ Protein-free CHO Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .267
Phalloidin, Alexa Fluor® 488 Conjugate . . . . . . . . . . . . . . . . . . . . . . . . . . . . 242 ProDoma™ Serum-free Hybridoma Media . . . . . . . . . . . . . . . . . . . . . . . . . . .277
Phosphate Buffered Saline (PBS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46 ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) . . . . . 269
PKLight® HTS Protein Kinase . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 230 - 231 Progenitor Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .87
pmaxFP® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192 HPGM™ Hematopoietic Progenitor Growth Medium . . . . . . . . . . . . . . . .87
pmaxFP®-Green . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .194 Neural Progenitor Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
pmax®-Yellow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195 NPBM™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
pmax®-Red . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196 NPDM™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
pmaxCloning™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 NPMM™ . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .97
Poietics® FAQs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121 ProHT™ Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
Poietics® Technical Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .122 ProNS0™ Chemically Defined, Protein-free Media . . . . . . . . . . . . . . . . . . . .279
Human Mesenchymal Stem Cell Media . . . . . . . . . . . . . . . . . . . . . . . . . .123 ProPer™ 1 Chemically Defined, Serum-free Medium . . . . . . . . . . . . . . . . . .271
Neural Progenitor Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 Prostate Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51 - 53
Osteoclast Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .124 PrEC – Human Prostate Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . .52
Preadipocyte Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .123 PrSC – Human Prostate Stromal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .52
Procedure for Thawing CD34+ and Precursor Cells . . . . . . . . . . . . . . . .122 PrSMC – Human Prostate Smooth Muscle Cells . . . . . . . . . . . . . . . 53, 67
PowerCHO® Chemically Defined, Serum-free CHO Media . . . . . . . . . . . . . 268 Prostate Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
PowerCHO® -GS Chemically Defined, Serum-free CHO Media . . . . . . . . . 268 PrEBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67
Indices
PPiLight® Inorganic Pyrophosphate . . . . . . . . . . . . . . . . . . . . . . . . . . 234 - 235 PrEGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52 - 53, 67

Preadipocyte Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 PrEGM™ SingleQuots® Supplements and Growth Factors . . . . . . . 53, 67
Preadipocyte Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91 ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
PrEC – Human Prostate Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 SCBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
Primary Cell Culture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 SCGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
Ascorbic Acid 25 .5 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 SCGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . .53
Bovine Brain Extract (BBE) 9 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78 SmBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .53
Bovine Pituitary Extract (BPE) 13 mg/ml . . . . . . . . . . . . . . . . . . . . . . . . . 78 SmGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67
363
Alphabetical Index
Continued
SmGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . .53 SmGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .53
PrSC – Human Prostate Stromal Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .52 Schneiders' Drosophila Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
PrSMC – Human Prostate Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . 53, 67 Sera . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 289 - 297
PyroGene® Recombinant Factor C Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . .329 Serum Supreme . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 295
PYROGENT® Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326 siRNA Test Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 200
PYROGENT® Plus Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 326 Skeletal Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58, 60
PYROGENT® Ultra Gel Clot LAL Assay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .327 NHAC-kn – Normal Human Articular Chondrocytes, Knee . . . . . . . . . .59
PYROSPERSE® Dispensing Agent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330 NHOst – Normal Human Osteoblasts . . . . . . . . . . . . . . . . . . . . . . . . . . . .59
Skeletal Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
■ Q Ascorbic Acid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Calcium Chloride Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
QCL-1000® Endpoint Chromogenic LAL Assay . . . . . . . . . . . . . . . . . . . . . . 328 CBM Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Quadratox 4 Species Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CDM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Baboon Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CDM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Canine Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CDM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .60
Human Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59, 60
Murine Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .85 CGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .60
Quick Reference - Primary Cells and Media . . . . . . . . . . . . . . . . . . . . . . . . 3 - 6 Chondrocyte ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . .60
Sodium Chloride Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
■ R OBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
OGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59, 60
Rat Cardiac Myocyte Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
OGM™ Differentiation SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . .60
RCGM Rat Cardiac Myocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
OGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . .60
Rat Cardiac Myocyte Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
RCBM Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
SkBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
RCGM BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Sodium Alginate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
RCGM SingleQuots® Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
Sodium Citrate Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Rat Hepatocytes – Sprague-Dawley & Media . . . . . . . . . . . . . . . . . . . . . . . . . 17
Skeletal Muscle Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61 - 63
Ready Heps™ Fresh Human Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
HSMM – Human Skeletal Muscle Myoblasts . . . . . . . . . . . . . . . . . . . . . .62
SkMC – Human Skeletal Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
. . . . . . . . . . . . . . . . . . . 20, 35, 39, 44, 48, 50, 53, 57, 60, 63, 68, 77, 78
Skeletal Muscle Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
REGM™ Renal Epithelial Cell Growth Medium . . . . . . . . . . . . . . . . . . . . . . . . . . 11
DPBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
REBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
REGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11, 55
SkBM®-2 Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
REGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . 11
SkGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62 - 63
Renal Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .54 - 57
SkGM®-2 SingleQuots® Supplements and Growth Factors . . . . . . . . . . 63
HRCE – Human Renal Cortical Epithelial Cells . . . . . . . . . . . . . . . . . . . . .55
SkGM® BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62, 63
HRE – Human Renal Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55
SkGM® SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . 63
NHMC – Normal Human Mesangial Cells . . . . . . . . . . . . . . . . . . . . . . . . .56
SkMC – Human Skeletal Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
RPTEC – Human Renal Proximal Tubule Epithelial Cells . . . . . . . . . . . .56
Smooth Muscle Cells & Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .64 - 68
Renal Epithelial Cell Model . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10, 11
AoSMC – Human Aortic Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . 65
Plated cells – polyester Transwell® Membranes . . . . . . . . . . . . . . . . . . 11
BdSMC – Human Bladder Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . 65
Renal Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
BSMC – Normal Human Bronchial Smooth Muscle Cells . . . . . . . . . . .66
MsBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
CASMC – Human Coronary Artery Smooth Muscle Cells . . . . . . . . . . . .66
MsGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .56 - 57
PASMC – Human Pulmonary Artery Smooth Muscle Cells . . . . . . . . . . 67
MsGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . 57
PrSMC – Human Prostate Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . 67
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
UASMC – Human Umbilical Artery Smooth Muscle Cells . . . . . . . . . . . .68
REBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
UtSMC – Human Uterine Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . .68
REGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .55 - 57
Smooth Muscle Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65 - 68
REGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . . 57
PrEGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Retronectin Dish . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78, 204
PrEBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Retronectin® Recombinant Human Fibronectin Fragment . . . . . . . . 78, 204
PrEGM™ SingleQuots® Supplements and Growth Factors . . . . . . . . . . . 67
rhTGF Beta 3 for hMSC Chondrogenic Differentiation
ReagentPack™ Subculture Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . .68
Media Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90
SmBM® Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 - 68
Richter’s CM Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258
SmGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22, 65 - 68
RPMI Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 258 - 259
SmGM®-2 SingleQuots® Supplements and Growth Factors . .22, 65, 68
RPTEC – Human Renal Proximal Tubule Epithelial Cells . . . . . . . . . . . . . . . .56
Sodium Chloride . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
rt NHEPS® – Rat Hepatocytes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Sodium Alginate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60
Indices
Sodium Chloride Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .60

■ S
Sodium Bicarbinate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
SAEC – Human Small Airway Epithelial Cells . . . . . . . . . . . . . . . . . . . . . . . . . 19 Sodium Pyruvate Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
SAGM™ Small Airway Epithelial Cell Growth Media . . . . . . . . . . . . . . . . . 19, 20 Specialty Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 261 - 279
SCGM™ BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37, 39, 52, 53 Cytogenetics Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273
SCBM™ Basal Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39, 53 Amniochrome® II Modified Medium . . . . . . . . . . . . . . . . . . . . . . . . .273
SCGM™ SingleQuots® Supplement Growth Factors . . . . . . . . . . . . . . . . . 39, 53 Amniochrome® Plus Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273
SmGM®-2 BulletKit® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53, 67 Amniochrome® Pro Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .273
364
Alphabetical Index
Continued
Hybridoma Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .274 Unprocessed Human Bone Marrow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83

HL-1™ Chemically Defined, Serum-free Media . . . . . . . . . . 274 - 275 UtMVEC-Myo – Human Uterine Microvascular Endothelial Cells . . . . . . . .34
ProDoma™ Serum-free Hybridoma Media . . . . . . . . . . . . . . . . . . .277 UtSMC – Human Uterine Smooth Muscle Cells . . . . . . . . . . . . . . . . . . . . . . . .68
UltraDOMA-PF™ Protein-free Hydridoma Media . . . . . . . . . . . . . . .277
UltraDOMA™ Serum-free Hybridoma Medium . . . . . . . . . . . . . . . .276 ■ V
Insect-XPRESS™ Protein-free Insect Cell Medium . . . . . . . . . . . . . . . . .278
IVF Culture Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272 Vectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .192
Human Tubal Fluid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272 pmaxCloning™ Vector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .197
Lymphochrome™ Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .272 pmaxFP® Fluorescent Protein Expression . . . . . . . . . . . . . . . . . . . . . . .192
PC-1™ Chemically Defined, Serum-free Medium . . . . . . . . . . . . . . . . . .263 pmaxFP®-Green Vectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .194
PowerCHO® Chemically Defined, Serum-free CHO Media . . . . . . . . . 268 pmaxFP®-Red Vectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
Pro293™ Chemically Defined, Serum-free Media . . . . . . . . . . . . . . . . .270 pmaxFP® Vector Backbone Information . . . . . . . . . . . . . . . . . . . . . . . . .193
ProCHO™ Protein-free CHO Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .267 pmaxFP®-Yellow Vectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .195
ProFreeze™-CDM, NAO, Chemically Defined Freeze Medium (2X) . . 269 Veronal Buffer (5X) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .287
ProNS0™ Chemically Defined, Protein-free Media . . . . . . . . . . . . . . . . .279 Versene® (EDTA) 0 .02% . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46, 284
ProPer™ 1 Chemically Defined, Serum-free Medium . . . . . . . . . . . . . .271 ViaLight® Cell Proliferation and Cytotoxicity . . . . . . . . . . . . . . . . . . . 223 - 225
ProVero™ 1 Serum-free Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .270 Vybrant® DiI Cell-Labeling Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
UltraCHO™ Serum-free CHO Cell Media . . . . . . . . . . . . . . . . . . . . . . . . . . 266 Visceral Preadipocyte Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
UltraCULTURE™ Serum-free Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262
UltraMDCK™ Chemically Defined, Serum-free Renal Cell Medium . . 269 ■ W
UltraMEM™ Reduced Serum Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 264
Water for Cell Culture . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284
X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Media 265
Waymouth's MB 752/1 Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
StellARray™ Gene Expression Systems . . . . . . . . . . . . . . . . . . . . . . . 209 - 216
Williams' Medium E . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
GeneSieve™ Query . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 210
WinKQCL® 4 Software Package . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
Global Pattern Recognition™ (GPR) Analysis Tool . . . . . . . . . . . . . . . . . 211
StellARray™ qPCR Arrays . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 211
Human Gene StellARray™ qPCR Arrays . . . . . . . . . . . . . 211, 212, 213
■ X
Mouse Gene StellARray™ qPCR Arrays . . . . . . . . . . . . . 214, 215, 216 X-VIVO™ Chemically Defined, Serum-free Hematopoietic Cell Media . . . .265
Subculturing Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90
DPBS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .90
Trypsin/EDTA (for use with human primary cells) . . . . . . . . . . . . . . . . .90
Subcutaneous Preadipocyte Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .91
SYTO® Green 11 Nucleic Acid Stain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
SYTOX® Green Nucleic Acid Stain . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 244
■ T
TC 100 Insect Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .259
TL HEPES Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .287
ToxiLight® Non-destructive Cytotoxicity . . . . . . . . . . . . . . . . . . . . . . 226 - 227
Transfection Reagents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .191
HiFect® . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .191
PrimeFect™ DNA and siRNA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 203 - 204
Transport™ Protein Delivery Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . 202
Transport™ Reagents FAQ's . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205
Transfection Services . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 342
Transport™ Protein Delivery Reagent . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 202
Transport™ Reagents FAQ's . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 205
Tris Buffer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 330
Trypan Blue . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .283
Trypsin 1:250 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284
Trypsin/EDTA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78, 284
Trypsin/EDTA (for use with human primary cells) . . . . . . . . . . . . . . . . . . . .90
Trypsin/EDTA Solution . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .46
Trypsin-Versene® (EDTA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284
Trypsin Neutralizing Solution (TNS) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46, 78
■ U
Indices
UASMC – Human Umbilical Artery Smooth Muscle Cells . . . . . . . . . . . . . . .68

UltraCHO™ Serum-free CHO Cell Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 266
UltraDOMA-PF™ Protein-free Hydridoma Media . . . . . . . . . . . . . . . . . . . . . . .277
UltraDOMA™ Serum-free Hybridoma Medium . . . . . . . . . . . . . . . . . . . . . . . .276
UltraGlutamine Supplement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 284
UltraMDCK™ Chemically Defined, Serum-free Renal Cell Medium . . . . . 269
UltraCULTURE™ Serum-free Medium . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .262
UltraSaline A . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .287
365
Notes
Notes
366
Notes
Notes
367
Notes
Notes
368
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Cell Discovery

Research Products Catalog

Clonetics® Primary Cells and Media

■ New! Amaxa® Nucleofector® Transfection Technology – unique technology for the

■ MycoAlert® Mycoplasma Detection System - detect mycoplasma contamination in your

The Lonza Cell Discovery Team

32 (0) 87 321 611 www.lonza.com/research

Ordering Information – Outside the U.S.

Lonza Walkersville, Inc.

Terms and Conditions

Warranty Product Use

Clonetics® Primary Cells & Media Introduction 81

Human 14 Neural Progenitor Cells Media 97

Rat 17 Custom Cell Isolation Services 98

Human Cells & Media

Animal Cells & Media

THESE PRODUCTS ARE FOR RESEARCH USE ONLY.

PRODUCT WARRANTY – CULTURES HAVE A FINITE LIFESPAN IN VITRO.

CD1 Mouse Brain - Mixed M-AsM-430 AGM™ CC-3186 76

Cells and Media

Rat Brain - Striatum R-CpAs-522 AGM™ CC-3186 75

Epithelial Cells (with Retinoic Acid)

Dermal - Neonatal CC-2509 FGM®-2 CC-3132 38

Periodontal Ligament CC-7049 SCGM™ CC-3205 37

Epidermal - Neonatal C2503A KGM®-2 CC-3107 42

Neural Progenitor Cells

Cells and Media

Rat Brain - Hippocampus R-Hi-501 PNGM™ CC-4461 74

CD133+ Cells 2M-102 2C-102 2L-102 87

Leukopak Peripheral Blood Cells 1A-125 83

Cells and Media

Clonetics® Media Products have been specially designed

express normal function and phenotype.

Available Cell Types 33°C – Large T-antigen Active Immortalization

— Human skeletal muscle cells

Corneal Epithelial Model Ordering Information

Renal Epithelial Cell Model

renal tubular epithelial monolayer as assessed through ■ Research Applications

g-GTP is expressed primarily on apical surface Cells Ordering Information

Recommended Medium: REGM™ BulletKit®

Cat. No. Size Price

100 Plated cells – polyester Transwell® Membranes

Media Ordering Information

REGM™ SingleQuots® Supplements and Growth Factors

0 Lot 1 Lot 2 Lot 3

Blood Brain Barrier Model

between the blood circulatory system and the brain. The

■ Research Applications ■ Other Non-Routine Testing

– Tight junction research – Permeability of [14 C] sucrose across the cell

Functional P-gp pump Cells Ordering Information

Cat. No. Size Price

Media Ordering Information

Cat. No. Size Price

EMVB SingleQuots® Supplements and Growth Factors

0 Lot No. 1 Lot No. 2 Lot No. 3 Lot No. 4

Permeability of [14 C] Sucrose across Clonetics® Bovine Brain Microvascular

hepatocyte cultures and performance is guaranteed.

■ Performance and Quality Tests

Human hepatocytes on Matrigel®

Human hepatocytes stained for albumin

Cells Ordering Information Media Ordering Information

Cat. No. Size Price Hepatocyte Culture Media