Group No.

4 School: Sorsogon National

High School

Names: Grade and Section: XII-STEM

Excellence

Impal, Mark Leo J. Subject: General Biology 1

Jesalva, Ayessa Collette D. Date of June
Martillano, Yaline J. Performance:

Santillan, Lester Jericho A. Date of July 1, 2019

Submission:

Teacher: Larry Domens Rating:

PLANT AND ANIMAL CELL VIEWING

Laboratory Activity Number 1

I. INTRODUCTION

When a part of living matter is examined under the microscope, it is seen to be

composed of very small units—which are termed as the cells. Cell are the smallest
units capable of basic life processes, such as taking in nutrients, expelling wastes and
reproducing. All living things are composed of it. There are only two main types of
cells and one of this is the eukaryotic cells which will be the focus. Under eukaryotic
cells are more different cell types, however plants and animals are the most familiar
eukaryotes wherein the former can be found on plants, and the latter can be found on
animals. Plant and animal cells have similarities and as well as differences on a variety
of aspects such as its components and shape. Cell is very small that it can only be
observed under a microscope, wherein it will be done in this activity.

II. OBJECTIVES
At the end of this activity, you should be able to:
 Describe the major features of a eukaryotic cell,
 prepare a wet amount of human cheek cells and rhoeo discolor cells,
 identify the different organelles of plant and animal cells visible under
compound light microscope,
 compare and contrast animal (cheek) cells and plant (Rhoeo dis color) cells.
III. MATERIALS
Materials Used:
*laboratory face mask *laboratory gloves *medicine dropper *iodine solution
*rheo discolor leaves *scalpel *methylene blue solution *toothpick
*slide *cover slip *microscope

IV. PROCEDURES
Plant Cells (Rhoeo discolor)
1. First, the student researchers prepared a wet amount of rhoeo discolor skin, took a
piece and obtained a thin layer of epidermal tissue.
2. Then they got a clean slide and place the obtained tissue on the slide.
3. After preparing the slide, they dropped iodine solution to stain. Wherein it allowed
the stain to remain for a few minutes. The staining was neither too dark nor too
light.
Caution: Iodine solution can stain clothes and skin
4. The cover slip was placed carefully to minimize ‘bubbles’. It was compressed
gently, and its excess liquid was removed.
5. The specimen was placed on the stage and was observed under 10X and 40X
respectively. After the cells were observed, it was documented (Figure 1-10X and
figure 2-40X) and labeled with all its visible components plus its indicated total
magnification power.
6. The slide was removed for the next specimen to be examined.

Animal Cells (Cheek)

1. First, a drop of methylene blue on the slide was put.

2. Next, the inside of a student researcher’s cheek was gently scraped using a
toothpick to smear the cells (tip of the toothpick) into the stain.
3. Over the sink, slide was tilted and was carefully rinsed with the excess stain bu
using a clean dropper. It allowed the stain to remain for a few minutes.
4. The cover slip was carefully placed to minimize ‘bubbles’ and was compressed
gently to remove excess fluid.
5. The specimen was placed on the stage and was under observation in 10X and 40X
respectively. Then, the cheek cells that are observed were documented(Figure 3-
 10X and Figure 4-40X) and labeled with all visible components plus its indicated
total magnification power.
6. Finally, the slide was removed and cleaning of the area was conducted.

GUIDE QUESTIONS:

1. Describe the cheek cells and rhoeo discolor cells in terms of

a. Size
b. Shape
c. Arrangement

Answer:

2. Identify and describe the structures present in both check and rhoeo discolor cells that are
visible under the compound light microscope.
Answer:
3. What organelle/s is/are present in a rhoeo discolor cell (visible under compound
microscope) that is/are not found in the cheek cell? Describe its structure and function.
Answer:
4. What structures (organelles) in both rhoeo discolor and cheek cells appear darker when
viewed under the microscope? Why is this so?
Answer:
5. What is the significance of the iodine and methylene blue in viewing cells under the
microscope?
Answer:
V. CONCLUSION
 VI. DOCUMENTATION

Figure 1.

Figure 2.

Figure 3.
References