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ORIGINAL PAPER
A. G. Gopala Krishna
123
J Am Oil Chem Soc
rather than being stored in the body [2]. Coconut oil pos- Co., St. Louis, USA. All reagents and chemicals used were
sesses antiviral, antibacterial and antiprotozoal properties of analytical grade.
due to the presence of C12:0 and C10:0 fatty acids [3].
Monounsaturated and polyunsaturated fatty acids are less Proximate Composition of Starting Materials
in coconut oil (&7–8 %) and, hence, it is highly stable
towards oxidation and eventually provides longer shelf-life The moisture content of the coconut samples was deter-
for the food products prepared in it [4]. mined according to the AOCS method Ac 2–41 [13]. Fat
Coconut oil contains minor components like tocophe- was extracted from dried coconut samples by using hexane
rols, tocotrienols, phytosterols and phenolics which are the in a Soxhlet apparatus according to AOCS O.M.No. Ac
natural antioxidants. Polyphenols are ubiquitous in food 3–44 [13]. The micro-Kjeldahl method was used to deter-
and are found to act as antioxidants, free radical scavengers mine total proteins described by the AOAC Official
and peroxidation inhibitors [5]. It also acts as an anti-car- Method 950.48 [14]. The method described by the AOAC
cinogenic and reduces cardiovascular diseases [6–8]. Phy- Official Method 950.02 [14] was used for crude fiber
tosterols are present predominantly in oilseed plants. In determination. Ash content of the dried and defatted
addition to nuts, they are found in legumes, vegetables and coconut samples was determined gravimetrically according
unrefined vegetable oils [9]. Tocopherols are fat soluble to AOCS O.M.No. Ba 5a-49 [13]. The iron, zinc, sodium,
antioxidants having vitamin E activity. Tocotrienols are potassium and calcium content of coconut samples were
present in coconut oil, palm oil and oil from bran sources analyzed by atomic absorption spectroscopy (AAS) [15].
such as rice bran and wheat bran. No other vegetable oils
contain tocotrienols in significant amounts. They act Acylglyerol Content of the Oil Samples by Column
against the membrane lipid peroxidation of polyunsatu- Chromatography
rated fatty acids [10].
Testa is the brown part covering coconut kernel, i.e., The TAG, DAG and MAG fractions from oil samples were
brown skin. It is obtained from coconut processing indus- separated according to the AOCS Official Method No. Cd
tries as a by-product through paring of wet coconut during 11c-93 [13].
the preparation of products like desiccated coconut, coco-
nut milk and virgin coconut oil. Testa is used as animal Fatty Acid Composition of Oil Samples by GC
feed. Consumption of coconut kernel provides the benefi-
cial effect on human health but the coconut testa is getting The fatty acid methyl esters of the fat extracted from the
wasted. coconut kernels and coconut testa were prepared by
Nevin and Rajamohan [11] have reported that the pre- transesterification according to the AOCS Method [13].
sence of phenolic compounds is mainly responsible for the Analysis was carried out using a gas chromatograph
antioxidant properties of virgin coconut oil. Seneviratne (model-GC-15A, Shimadzu Corporation, Kyoto, Japan)
et al. [12] have reported that the final phenolics content of equipped with a FID detector and a stainless steel column
coconut oil depends on the components of the endosperm. of 3 m length 9 0.5 mm ID, coated with 15 % diethylene
Information on the components of coconut testa is rare. glycol succinate on 60–80 mesh chromosorb WAW. The
Hence, the study focuses on the evaluation of composition operating conditions were as follows: nitrogen flow 40 ml/
and minor components including tocopherols, tocotrienols, min, hydrogen flow 40 ml/min, air flow 300 ml/min, col-
phytosterols and phenolics of oil from coconut testa in umn temperature 180 °C, injector temperature 220 °C and
comparison with coconut whole and coconut white kernel. detector temperature 230 °C. A reference standard FAME
mix (Supelco Inc., Bellefonte, PA, USA) was analyzed
under the same operating conditions to determine the peak
Materials and Methods identity. The FAMEs were expressed as relative area %.
Wet coconut whole (WCW) and copra whole (CW) were Triacylglycerols Composition of Oil Samples by HPLC
purchased from the local market. The white kernel and
testa of wet coconut and copra were separated manually to Triacylglycerols composition was determined according to
get wet coconut white kernel (WCWK) and wet coconut Swe et al. [16]. The HPLC system used was equipped with
testa (WCT); copra white kernel (CWK) and copra testa a Shimadzu LC-10A liquid chromatograph with a Refrac-
(CT). Standard gallic acid, cholesterol, FAME mix, toc- tive Index (RI) detector. The column used was a C18
opherols, hydroxybenzoic acid, chlorogenic acid, vanillic Discovery column (Supelco, Bellefonte, USA) with a
acid, syringic acid, coumaric acid, caffeic acid, ferulic acid mobile phase acetone/acetonitrile (70:30, v/v) at the flow
and cinnamic acid were procured from Sigma Chemicals rate of 1 ml/min. The oil samples were dissolved in
123
J Am Oil Chem Soc
acetone. TAG peaks were identified based on the retention 4.6 mm, 5 lm) Bondapack column. Isocratic elution was
time of the TAG standards. carried out with a mobile phase consisting of water:
methanol (82:18 v/v) containing 2 % (v/v) acetic acid, at a
Phenolics Extraction from Oil Samples flow rate of 1 ml/min. A photo diode array (PDA) detector
was used for detection of phenolic acids and the HPLC
The phenolics extraction from the samples was carried out profiles were obtained at 280 and 320 nm. The injection
according to Seneviratne and Dissanayake [17]. The volume for all samples was 10 ll. Identification of phe-
phenolics were extracted using methanol: water (80:20 nolic acids was based on retention times in comparison
v/v). About 5 g of the oil sample was mixed with 1 ml of with standards [21].
80 % methanol and vortexed for 2 min (twice). The sam-
ples were centrifuged at 2,500 rpm for 10 min at room
Analysis of Tocopherols and Tocotrienols of Oil
temperature. The methanol: water layer was collected in
Samples by HPLC
another tube. This step was repeated four times and the
extracts pooled were made up to 4 ml with 80 % methanol.
Tocopherols and tocotrienols were estimated in the oil
samples by HPLC according to the AOCS method O.M.No.
Determination of Total Phenolics Content (TPC) of Oil
Ce 8–89 [13]. The analysis of tocopherols and tocotrienols
Samples by the Colorimetric Method
was achieved by normal phase HPLC separation on a silica
column (Lichrosorb Si60 5 lm) employing Shimadzu
The total phenolics content (TPC) was determined using
HPLC system consisting of a LC-10A pump, injector fitted
the Folin–Ciocalteu reagent. Different aliquots were mixed
with a 20 ll loop and fluorescence detector (FLD). The
with 0.2 ml of Folin–Ciocalteu reagent and were kept for
mobile phase was hexane: isopropyl alcohol (99.5:0.5, v/v)
3 min. About 1 ml of 15 % Na2CO3 solution was added
at the flow rate of 1 ml/min. The excitation wavelength of
and made up to 7 ml with distilled water. The tubes were
290 nm and an emission wavelength of 330 nm were kept
incubated for 45 min and centrifuged at 2,000 rpm for
for the fluorescence detection of all the peaks. The toc-
10 min at room temperature. The absorbance was read at
opherols and tocotrienols were identified using standard
765 nm using a UV–Visible spectrophotometer (Shimadzu
tocopherols and expressed as a-tocopherol.
corporation, Kyoto, Japan, model UV—1601). The TPC
(mg/100 g) was calculated using gallic acid as standard
compound [18]. Statistical Analysis
Determination of Total Phytosterols Content of Oil All the analyses were carried out in triplicate and the
Samples by the Colorimetric Method average values ± SD are reported. One-way ANOVA was
used to calculate significant differences among the coconut
Total phytosterols content was analyzed by using the Lie- and oil samples [22]. A two-tailed p value was determined
bermann-Burchard method. About 0.3 g of oil sample was to show the significant differences at a p value B0.001.
dissolved with 1.2 ml of chloroform and 2 ml of the Lie-
bermann–Burchard reagent (0.5 ml of sulphuric acid dis-
solved in 10 ml of acetic anhydride, covered and kept in an Results and Discussion
ice bucket). Final volume was made up to 7 ml with
chloroform and mixed well. The tubes were kept in the Proximate Composition of Starting Materials
dark for 15 min and their absorbance was read at 640 nm
using a UV–Visible spectrophotometer (Shimadzu corpo- The composition of CW, CWK, CT, WCW, WCWK and
ration, Kyoto, Japan, model UV—1601). A blank was WCT were analyzed and are presented in Table 1. The
prepared without the sample. The total phytosterols content composition included moisture content, fat content, protein
was calculated using standard cholesterol [19]. content, carbohydrate content, crude fiber content and ash
content. The moisture content was less in copra (3.8, 4.0
Analysis of Individual Phenolic Acids of Oil Samples and 4.3 %) when compared to the wet coconut (43.5, 32.9
by HPLC and 42.2 %). WCT contained a lower moisture content
than WCW and WCWK. Solangi et al. [23] had reported
The extraction of phenolic acids from coconut oils for that the moisture content of mature coconut ranges from 38
HPLC analysis was done according to Brenes et al. [20]. to 62 % and ash content 0.85–1.26 % for different coconut
The analysis of phenolic acids in the coconut oil was varieties. According to Obasi et al. [24], the moisture
conducted using a Waters Atlantis C18 (250 mm 9 i.d. content of copra was 7.51 %, crude fiber was 7.70 % and
123
J Am Oil Chem Soc
CW 4.3 ± 0.18a 59.8 ± 0.32a 10.2 ± 0.21a 24.3 ± 0.78a 7.0 ± 0.64a 1.4 ± 0.04a
a b b b b
CWK 3.8 ± 0.02 63.6 ± 0.4 8.1 ± 0.35 22.4 ± 0.64 6.6 ± 0.14 2.1 ± 0.06b
a a c c a
CT 4.0 ± 0.01 59.0 ± 0.95 9.3 ± 0.28 26.3 ± 0.78 11.6 ± 0.35 1.4 ± 0.05a
WCW 42.2 ± 0.18b 37.0 ± 0.22c 7.5 ± 0.35b 12.3 ± 0.28d 14.3 ± 0.57c 1.0 ± 0.04c
c d d e b
WCWK 43.5 ± 0.54 38.8 ± 0.46 6.2 ± 0.21 10.6 ± 0.35 11.7 ± 0.35 0.9 ± 0.06d
d e b a d
WCT 32.9 ± 0.21 34.7 ± 0.5 7.1 ± 0.42 24.6 ± 0.85 17.2 ± 0.42 0.7 ± 0.03e
Values are mean ± SD of wet coconut and copra from five individual nuts separately. All analyses carried out in triplicate (n = 15 for five
individual nuts). All are expressed on a wet basis. The values with different superscripts in the column are significantly different at p \ 0.001
CW copra whole, CWK copra white kernel, CT copra testa, WCW wet coconut whole, WCWK wet coconut white kernel, WCT wet coconut testa
CW 120.3 ± 1.0a 15.5 ± 0.5a 14.6 ± 0.6a 7.9 ± 0.5a 2.9 ± 0.5a
CWK 124.1 ± 1.0b 21.0 ± 0.6b 18.1 ± 0.2b 3.2 ± 0.1b 1.6 ± 0.2b
CT 120.3 ± 1.3a,c 22.4 ± 0.8c 17.0 ± 1.0b,c 6.2 ± 0.3c 3.0 ± 0.1a,c
WCW 122.1 ± 0.8a,b,c 21.6 ± 0.4b 18.1 ± 0.4b,c 7.9 ± 1.0a 2.2 ± 0.1b,c
bc b a d
WCWK 123.8 ± 0.5 20.3 ± 0.2 14.0 ± 0.3 1.5 ± 0.3 1.8 ± 0.4b,c
d d c d
WCT 107.8 ± 0.6 29.8 ± 0.9 16.7 ± 0.6 1.9 ± 0.3 1.6 ± 0.1b,c
Values are mean ± SD of wet coconut and copra from five individual nuts separately. All analyses carried out in triplicate (n = 15 for five
individual nuts). All are expressed on a wet basis. The values with different superscripts in the columns are significantly different at p \ 0.001.
CW copra whole, CWK copra white kernel, CT copra testa, WCW wet coconut whole, WCWK wet coconut white kernel, WCT wet coconut testa.
K potassium, Na sodium, Ca calcium, Fe iron, Zn zinc
fat content was 47.80 %. The chief constituent of coconut Mineral Composition of Coconut Samples
kernel was carbohydrate, followed by lipid. On the wet
basis, the fat content of copra was high ranging from 59.8 The mineral composition of coconut samples is presented
to 63.4 % and the wet coconut was low ranging from 34.7 in Table 2. The coconut testa and kernel have substantially
to 38.8 %. CT had the same amount of fat when compared more potassium than sodium. CW, CWK, CT, WCW,
to CW and CWK. The crude fiber content of copra was less WCWK and WCT had 120.3, 124.1, 120.3, 122.1, 123.8
(6.6–11.6 %) and wet coconut was more (11.7–17.2 %). and 107.8 mg of potassium/100 g, respectively. They had
Both CT (11.6 %) and WCT (17.2 %) had high crude fiber 15–29 mg/100 g of sodium, 14–18 mg/100 g of calcium,
content. The crude fiber content of healthy coconut kernel 1.5–7.9 mg/100 g of iron and 1.6–3 mg/100 g of zinc
was 13.13 % [25]. The ash content of copra was slightly content. CT contained 29.8 mg/100 g of sodium content.
higher than that of the wet coconut. CW had an ash content CW, CT and WCW contained 7.9, 6.2 and 7.9 mg of iron
of 1.4 %, CWK was 2.1 % and CT was 1.4 %. WCW had content/100 g, respectively. CW (2.9 mg/100 g) and CT
an ash content of 1.0 % of which WCWK was 0.9 % and (3.0 mg/100 g) contained slightly high zinc content. CT
WCT was 0.7 %. WCT had less ash content when com- (22.4 %) and WCT (29.8 %) had more sodium content than
pared to WCW and WCWK. CWK had more ash content in other coconut samples. The results are in good agree-
than in CW and CT. Santoso et al. [26] showed that the ash ment with that reported by Solangi et al. [23].
content of wet coconut and copra (dry matter) was 1.15 and
2.11 %, respectively. The protein content of CW, CWK, Acylglycerol and Fatty Acid Compositions of Oil
CT, WCW, WCWK and WCT were 10.2, 8.1, 9.3, 7.5, 6.2 Samples
and 7.1 % respectively [23, 24]. The carbohydrate content
was calculated by difference. The carbohydrate content of The acylglycerol and fatty acid compositions of oil samples
WCT (24.6 %) and CT (26.3 %) was more than other are presented in Table 3. CW, CWK, CT, WCW, WCWK
coconut samples (10.6–24.3 %). and WCT oils had a TAG content of 90–98.2 %, DAG of
123
J Am Oil Chem Soc
1–8 % and MAG of 0.4–2 %. CW contain a high amount WCT oils (82.51–71.59 %) was marginally less when
of DAG, i.e. 8.4 %, CT oil with 5.3 % and WCT oil with compared to CW, CWK, WCW and WCWK oils
3.2 %. WCWK oil contained a high amount of TAG (92–95 %); MUFA of CT was 12.19 %, WCT oils was
(98.2 %) and CW oil was 90.00 %. Oils from wet coconut 17.82 % and CW, CWK, WCW and WCWK oils were
(96.4–97.7 %) contained a slightly high TAG content when 4–6 %; PUFA of CT and WCT oils was 5.32 % and
compared to that of copra oils (90–94.1 %). Whereas for 10.6 %, respectively, and CW, CWK, WCW and WCWK
DAG content, it was more in CW oil (8.4 %) and less in oils were 1–2 %. MCFA was less in CT (48.25 %) and
WCWK oil (1.1 %). DAG content was higher in copra WCT (36.16 %) oils whereas CW, CWK, WCW and
(4.6–8.4 %) than in wet coconut (1.1–3.2 %). MAG con- WCWK oils were 64–68 %. The CT and WCT oils have
tent was also slightly higher in copra (0.6–2.0 %) when higher PUFA and MUFA as compared to other coconut oil
compared to the wet coconut (0.4–0.6 %). DAG is used in samples. The low SFA and MCFA in CT and WCT are
small quantity in foods as an emulsifier [27, 28]. most probably due to the presence of a high amount of
The fatty acid compositions of coconut oil samples are MUFA and PUFA than in other samples. Oils from CT and
reported in Table 3. Lauric acid is the major fatty acid of WCT were significantly different from other coconut oil
coconut oil [1]. The lauric acid content of CT and WCT samples in the fatty acids composition. The fatty acids
oils was 40.94 and 32.4 %, respectively whereas CW, composition of whole coconut kernel oil was in good
CWK, WCW and WCWK oils were 50–53 %. The caprylic agreement with that reported by Bhatnagar et al. [3].
acid (C8:0) and capric acid (C10:0) content of CT and WCT
oils were slightly less when compared to other oil samples. Triacylglycerol Composition of Oil Samples
Palmitic acid (C16:0) content of CT (11.31 %) and WCT
(14.07 %) oils were higher than the other oil samples, The major TAGs in the coconut oils were CCLa, CLaLa,
which is varying from 6.8 % for WCW to 7.4 % for LaLaLa and LaLaM; these counted for around 50–75 % of
WCWK and CWK oils. MCW (2.3 %) had more linolenic the total TAG composition (Table 4). The sum of these
acid (C18:0) followed by CWK (1.9 %), WCWK (1.9 %), four TAGs in CW, CWK, CT, WCW, WCWK and WCT
CT (1.6 %), WCT (1.2 %) and CW (1.1 %). CT and WCT oils were 51.82, 64.96, 50.4, 74.61, 76.67 and 39.97 %,
oils contained 12.19 % and 17.82 %, respectively, of oleic respectively. WCT oil showed comparatively slightly
acid (C18:1) and 5.32 and 10.6 %, respectively, of linoleic higher LaLaO (6.42 %), LaMO (8.04 %), LaMP (8.0 %),
acid (C18:2) content whereas CW, CWK, WCW and LaSO (3.46 %), MOO (3.48 %), MPO (2.79 %), OOO
WCWK oils contained 4–6 % of C18:1 and 1–2 % of C18:2. (3.35 %), POO (2.22 %) than CWK, CT, CW, WCWK and
The oils from CT and WCT were found to be having more WCW oils. CCLa (4.64 %), CLaLa (8.56 %), LaLaLa
C18:1 and C18:2 than other oil samples. The SFA of CT and (12.34 %) and LaLaM (14.43 %) were less in WCT oil as
123
J Am Oil Chem Soc
Table 4 Triacylglycerol composition of oils from coconut kernel and The TAG composition of wet coconut kernel and copra
testa were similar to that of what Gopala Krishna et al. [1] had
Triacylglycerol CW CWK CT WCW WCWK WCT reported. The TAG composition of oils from testa showed
% some differences when compared to other oils.
UI 0.38 0.4 0.13 – – –
Composition of Oil Samples
UI 0.83 1.61 1.55 1.09 – –
UI 0.77 2.01 0.54 0.04 – 0.32
In the coconut industry, the testa is removed during oil
UI – 1.44 0.64 – – –
extraction as it gives a yellowish color to oil. The oils
CpCC 1.23 1.88 0.75 0.49 0.14 0.19
extracted along with the testa gave little higher phenolics
CpCpLa 1.21 1.42 1.68 1.44 0.95 0.29
content than the oil extracted without testa. Nutraceuticals
CpCLa 2.24 2.91 7.91 5.26 4.17 0.93
content and compositions of oil samples are presented in
CCLa 7.41 12.12 12.20 19.69 16.26 4.64
Table 5. The total phytosterols content of CW, CWK, CT,
CLaLa 12.18 16.45 18.76 23.53 20.14 8.56
WCW, WCWK and WCT oil were 49.89, 33.31, 42.52,
LaLaLa 16.23 20.01 14.30 23.04 22.28 12.34 50.27, 30.66 and 50.97 mg/100 g respectively. The total
LaLaM 16.0 16.38 5.04 10.41 15.93 14.43 phytosterol content was more in CW (49.89 mg/100 g) and
LaLaO 3.87 3.07 13.42 1.73 2.37 6.42 WCW (50.27 mg/100 g) than in CWK (33.31 mg/100 g)
LaMM 13.73 9.77 4.91 5.33 8.91 15.9 and WCWK (30.66 mg/100 g). This was may be due to the
LaMO 4.62 2.93 6.21 1.54 1.33 8.04 presence of testa while extracting the oil. The total phy-
LaMP 6.81 4.86 0.29 2.70 3.85 8.00 tosterols content of oils from CT and WCT was almost
MPL 0.54 0.33 1.92 0.08 0.20 0.18 similar to that of oils from CW and WCW whereas the oils
LaCO 0.26 0.60 1.46 0.83 0.84 2.75 from CWK and WCW were less when compared to other
LaPP 1.66 0.97 1.43 0.47 1.12 1.15 coconut oils. Raja Rajan et al. [29] had reported that the
LaSO 1.45 0.10 1.32 – 0.16 3.46 total phytosterols content of whole coconut oils was
MOO 1.97 0.54 1.10 0.87 0.19 3.48 87 mg %.
MPO 1.26 0.22 0.89 0.56 0.62 2.79 Oil samples showed fewer amounts of phenolics. CW,
OOO 1.53 – 0.13 0.53 0.14 3.35 CWK, CT, WCW, WCWK and WCT had 1.1, 1.9, 1.4, 0.2,
POO 1.23 – – 0.20 – 2.22 0.5 and 0.7 mg of TPC/100 g oil, respectively. TPC was
POP – – – 0.18 – 0.17 more in the copra than in the wet coconut. Henna et al. [30]
UI-unidentified mono and diacylglycerols. Values provided are mean
reported that virgin coconut oil contained 0.65 mg of total
of triplicate samples and the cv is \1 % phenolics/100 g of oil. All the samples showed the pre-
CW copra whole, CWK copra white kernel, CT copra testa, WCW wet sence of gallic acid, hydroxybenzoic acid, vanillic acid,
coconut whole, WCWK wet coconut white kernel, WCT wet coconut syringic acid, coumaric acid, caffeic acid, ferulic acid and
testa cinnamic acid in significant amounts. The total phenolic
acids content of oils from WCT and CT (388.9 and
compared to other samples. The trilaurin (LaLaLa) is less 313.1 lg/100 g, respectively) were higher when compared
in oils from WCT (12.34 %) and CT (14.30 %) whereas to that of the WCW, WCWK, CWK and CW oils (291.1,
WCW, CW, CWK, WCWK showed 23.04, 16.23, 20.01 94.7, 141.8 and 131.1 lg/100 g of oil, respectively).
and 22.28 %, respectively. CT oil contained the least Among the phenolic acids, coumaric acid content was
amount of dilaurin (LaLaM) (5.04 %) than CWK found to be more in WCT oil (230.6 lg/100 g) and hy-
(16.38 %), CW (16.0 %), WCWK (15.93 %), WCT droxybenzoic acid content in CT oil (126.4 lg/100 g).
(14.43 %) and WCW (10.41 %) oils. WCT oil had a low Seneviratne et al. [31] had reported the same phenolic acids
amount of CCLa (4.64 %) and CLaLa (8.56 %). WCT oil composition in coconut kernel. The results are in agree-
(8.04 %) and CT oil (6.21 %) showed comparatively ment with the reports of Seneviratne and Dissananyake
higher LaMO content than oils from CWK (2.93 %), CW [17]. Marina et al. [32] had reported that the phenolic acids
(4.62 %), WCWK (1.33 %) and WCW (1.54 %). CT oil composition of coconut kernel oil varies with different
had 0.29 % and WCT oil had 8.00 % of LaMP content. extraction methods and contained protocatechuic acid,
MPL was marginally more in CT oil (1.92 %) and very less vanillic acid, caffeic acid, syringic acid, ferulic acid and p-
in CWK (0.33 %), CW (0.54 %), WCWK (0.20 %), MCT coumaric acid. Phenolic acids were found to be more in
(0.18 %) and WCW (0.08 %) oils. Triolein content was mature coconut than in copra. The oils from testa were
more in WCT oil (3.35 %) whereas CT, CW, WCWK and found to have higher total phenolic acids than CW and
WCW oils had 0.13, 1.53, 0.14 and 0.53 % respectively. WCW followed by CWK and WCWK.
123
J Am Oil Chem Soc
Total phytosterols (mg/100 g) 49.89 ± 1.9a 33.31 ± 2.1b 42.52 ± 0.9c 50.27 ± 1.5a 30.66 ± 0.2b 50.97 ± 0.1a
a b c d e
TPC (mg/100 g) 1.4 ± 0.19 1.1 ± 0.11 1.9 ± 0.12 0.7 ± 0.02 0.2 ± 0.04 0.5 ± 0.02d
Phenolic acids (lg/100 g)
Gallic acid 24.7 ± 1.2a 30.3 ± 1.1b 32.1 ± 2.1b 103.9 ± 2.2c 15.9 ± 0.7d 12.6 ± 2.8d
a b c c d
Hydroxybenzoic acid 7.6 ± 0.1 94.1 ± 0.9 126.4 ± 0.6 127.4 ± 1.7 34.7 ± 1.1 55.1 ± 1.1e
a
Vanillic acid 63.8 ± 0.3 nd nd nd nd nd
Syringic acid 17.9 ± 0.4a nd nd nd 37.3 ± 1.1b 26.6 ± 0.9c
a a b b
Coumaric acid 10.0 ± 0.7 11.2 ± 1.1 42.1 ± 3.1 48.9 ± 0.0 nd 230.6 ± 3.5d
Caffeic acid 3.1 ± 0.5a 4.9 ± 0.3a 12.8 ± 1.3b nd nd 27.4 ± 2.0c
a b b
Ferulic acid 1.7 ± 0.5 nd 47.5 ± 2.04 5.4 ± 0.4 nd 5.0 ± 1.0c
a a b c d
Cinnamic acid 2.4 ± 0.9 1.3 ± 0.6 4.1 ± 0.8 9.8 ± 0.3 6.9 ± 0.9 31.7 ± 0.2e
Total 131.2 141.8 313.9 291.4 94.8 389.0
Tocopherols (mg/100 g)
aT 0.6 ± 0.02a nd nd 3.6 ± 0.0b 2.5 ± 0.0c 0.04 ± 0.0d
b ? cT 2.1 ± 0.4a 3.7 ± 0.0b 2.8 ± 0.2c nd nd 4.0 ± 0.1b
dT nd nd nd nd nd 2.9 ± 0.4a
a b c a
aT3 0.2 ± 0.0 2.1 ± 0.2 16.6 ± 2.4 0.1 ± 0.0 Trace 90.2 ± 5.0d
a b a
b ? cT3 Trace 0.9 ± 0.1 2.2 ± 0.1 0.4 ± 0.0 nd 2.9 ± 0.6c
a a
dT3 nd nd 0.7 ± 0.0 0.3 ± 0.1 nd nd
a a b a a
Total (T ? T3) mg/100 g 2.9 ± 0.4 6.7 ± 0.5 22.3 ± 1.6 4.4 ± 0.8 2.5 ± 0.0 100.1 ± 8.5c
All analyses carried out in triplicates (n = 15 for five individual nuts). The values with different superscripts in the rows are significantly
different at p \ 0.001.
CW copra whole, CWK copra white kernel, CT copra testa, WCW wet coconut whole, WCWK wet coconut white kernel, WCT wet coconut testa.
TPC and total phytosterol content by the colorimetric method and expressed as gallic acid and cholesterol, respectively, nd not detected
The coconut kernel oils contained less tocopherols extracted from coconut testa were found to be rich in
(T) and tocotrienols (T3) content when compared to oils minor components like tocopherols, phenolics and phy-
from testa. The T and T3 content of CW, CWK, WCW and tosterols than oil extracted from kernel or whole coconut.
WCWK oils were only 2.9, 6.7, 4.4 and 2.5 mg/100 g of They contained &50 mg of total phytosterols/100 g of
oil, respectively. However, CT and WCT oils had 22.3 and oil. CT and WCT oils contained slightly higher DAG
100.1 mg/100 g oil of T and T3 content. CW (2.1 mg/ content than WCWK, WCW and CWK oils. WCT oil
100 g) and CWK (3.7 mg/100 g) oils had more of b ? c contained more amounts of coumaric acid whereas CT oil
tocopherols content whereas WCW (3.6 mg/100 g) and was found to have more of hydroxybenzoic acid than CW,
WCWK (2.5 mg/100 g) oils had more of a-tocopherol CWK, WCW and WCWK oils. The oil from WCT had
content. Oils from CT and WCT were found to have higher less trilaurin content and slightly higher triolein content
tocotrienols concentration than other coconut oil samples. when compared with other coconut oil samples. To our
There was 90.2 mg/100 g for WCT oil followed by CT oil, knowledge, reports on composition and natural antioxi-
16.6 mg/100 g. WCT oil contained 2.9 mg/100 g of d- dants/minor components of oil from coconut testa have
tocopherol content. CT oil contained 0.7 mg/100 g of d- not been reported so far. Dried CT and WCT yield same
tocotrienols content. Raja Rajan et al. [29] had reported amount of fat like copra and dry coconut kernel; there-
that whole coconut oil contained 1.7–3.1 mg/100 g of total fore, they can also be used as a good oil source. Oils
tocopherols and tocotrienols content. extracted from WCT and CT could be used for regular
consumption as they contain a similar fatty acid compo-
sition, acylglycerol and triacylglycerol profile, minor
Conclusion components like phenolics, phytosterols, tocopherols and
tocotrienols for beneficial health effects.
Coconut testa and kernel samples are rich in potassium
Acknowledgments The authors are thankful to Director CSIR-
content. The CT and WCT oils have higher PUFA and CFTRI, Mysore for providing infrastructural facilities and The
MUFA as compared to other coconut oil samples. Oils Coconut Development Board, Kochi, for funding the project.
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J Am Oil Chem Soc
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