EFFECTIVENESS OF AZOLLA PLANT (Azolla pinnata) USE AS AN

ALTERNATIVE FEEDS FOR BROILER CHICKEN (Gallus Domesticus)

A Research Paper Presented to the Faculty of

AGUSAN NATIONAL HIGH SCHOOL

Senior High School Department,

Butuan City, Philippines

In Partial Fulfillment of the Requirements for the Subject

Research Capstone

by

Raymart B. Monteclaro

Kevin Gio A. Palarca

Marry May F. Razon

Jayrico L. Llanos

Ian S. Baclayon

July , 2019
 CHAPTER 1

THE PROBLEM AND REVIEW OF LITERATURE

Background of the Study

Poultry manufacturers around the sector retain to war

with excessive manufacturing charges for rooster feeding,

in large part due to volatility in expenses for simple feed

ingredients (Zootecnica, 2018). Commercially raised

chickens are fed compounds that can be dangerous to human

health, reports a study published inside the magazine

“Environmental health views” in April 2008. Some businesses

have discontinued use of these compounds, but commercially

raised chickens might also nevertheless be eating feed that

includes parts of slaughtered chickens, feces, plastics and

an overabundance of grains. A few feed additionally

contains arsenic, which facilitates make hen meat pink, but

could also be risky to human health. Poor first-class feed

could imply negative best meat (Cespedes, 2010).

Consistent with Arcuri (2019), Chick starter,

precisely what it sounds like, chick starter is for the

primary (generally six) weeks of your child chicks' lives.

That is normally 22 to 24 percentage protein for meat birds

(known as broiler starter) and 20 percentage protein for

laying breeds. You could buy medicated or unmedicated chick

starter. The majority use a medicated feed, however natural

and pastured small farms frequently use unmedicated feed

broiler rations. Those high-protein feeds are for meat

birds, specifically Cornish X Rock crosses that grow

extraordinarily speedy. Broiler rations are usually 18 to

twenty percent protein. This is every now and then referred

to as "grower-finisher" feed. For historical past and

pastured meat birds, protein content material may be

decreased to 16 percentage after 12 weeks of age until

butchering. A few may also pick out to keep the background

meat birds at the higher grower-finisher rations until

slaughter.

Pullet Developer or Finisher. At 14 weeks, younger

pullets may be lowered to a sixteen percentage protein feed

till they start laying. A few feed lines don't distinguish

between this level and the grower stage and just have a

grower-finisher that is someplace inside the center

protein-wise.

In the recent past, few studies have been carried out

in chicken to assess the effect of Azolla meal and

raw Azolla feeding on the performance of chicken. Its

effectiveness use unconventional feed stuffs

like Azolla without reducing the performance, it will

increase the profitability of chicken business. Hence, a

study was carried out to evaluate the effect of

dried Azolla plant vis-a-vis raw Azolla as choice feeding

on the growth. Azolla is very rich in proteins, essential

amino acids, vitamins (vitamin A, vitamin B12, Beta

Carotene), growth promoter intermediaries and minerals like

calcium, phosphorous, potassium, ferrous, copper, magnesium

etc. Azolla, on a dry weight basis, is constituted of 25-

35%.protein

In order to know the effectiveness of Azolla plant as

an alternative commercial feeds for chickens. The

connection between Azolla plant and chickens and its yield

and nutrients and a low costs of money.

In conclusion, our proposed project is more effective

and affordable for the production of chicken broiler in

poultry farms.
Research Objectives

This study is about azolla plant as an alternative

feeds for chicken. Specifically, it aims to study the

effectiveness of using azolla plant for the growth of

chicken at low cost at the same time more yield than

commercial feeds.

1. To determine the significant effects of adding azolla

plant as an alternative commercial feed for chickens.

2. To determine the benefits of using azolla plant as an

alternative feed for chickens.

3. To compare the effectiveness of azolla plant as an

alternative commercial feed for chicken.

Statement of the Problem

The main purpose of the study is to determine the

effectiveness between azolla plants and commercial chicken

feeds. Based on the foregoing major research problem, the

specific problems are as follows:

1. What is the level of effectiveness using azolla plant as

an alternative commercial feeds?

2. What is the mean weight of chicken using azolla plant

as an alternative commercial feeds?

3. Is there a significant difference between azolla plant

as an alternative feed to commercial feeds?

Statement of the Hypothesis

Null Hypothesis (Ho)

The hypotheses of this study highlights the

effectiveness of azolla plant as an alternative feed

against commercial chicken feeds.

1. There is no level of effectiveness using azolla

plants as an alternative commercial feeds.

2. The mean weight of chickens using azolla plant as

an alternative commercial feeds is zero.

3. There is no significant difference between azolla

plant and commercial feeds.

Theoretical Framework

Azolla plant has been used for many years as an

alternative feeds for livestock throughout Asia, South

Africa. Using Azolla plant containing high content of

protein, essential amino acid, vitamins (Vitamin A, Vitamin

B12, Beta Carotene). Growth promoter intermediaries and

minerals. (Kamalasanana , 2010)

Conceptual Framework

The independent variable is a pre-test data of the

broiler chicken yield which the chicken feed of azolla

plant. Temperature, climate, location serves as intervening

due to the fact that temperature and climate can affect the

growth of the chicken as well as the location. The

dependent variable is the result of this process.

This study focuses on the effectiveness of using

Azolla plant as an alternative feed against commercial

feeds. The materials that will be used are poultry

 facilities and essential equipment such as chicken poultry

house, waterers, feeding troughs 5watts incandescent bulb,

pail, stick broom, clean empty sacks, record book and

weighing scale.

Independent Intervening Dependent

Variables Variables Variables

• azolla plants • Temperature • Yield

(Azolla Plant)
• commercial feeds • Climate
• Yield
• Location (Commercial feed)

Figure 1. Schematic Diagram of the interrelationship among

variables used in the study.
Research Paradigm

The azolla plant will be used as feeds for broiler

chicken. The drying of azolla plant will processed through

air to prevent volatilization at least ¾ day before mixing

to commercial feeds. The yield of the chicken will depend

on the amount of the feeds.

Experimental Azolla Broiler

Chicken Feeds

Yield

Commercial
Control
Broiler Chicken
Feed

Figure 2. Paradigm of Azolla Plant (Azolla pinnata) Use as

an Alternative Feeds for Broiler Chicken

Scope of Limitation

This study is limited only to the use of azolla plant

as an alternative feed for boiler chicken, located at

Magallanes, Dahican, Agusan Del Norte. The further

experimentation of the subjects will conducted in Butuan

City. During the experimentation there will only 10 boiler

chicken shall be taken up. The researchers will only use

50% of existing feed and adding of 50% of azolla plant. It

will be expected that the experiment will take a 4 weeks

including preparatory process. After the experiment the

researcher will send the experimented specimens and shall

be subjected for analysis and result.

Significance of Study

This study can determine the effectiveness of Azolla

plant (Azolla pinnata) as an alternative commercial feed

for the chicken. Aside from its high protein the Azolla

plant is also low cost and abundant it can easily grow in

ditches, ponds and Azolla cultivation. The researchers

believe that the following would benefit from the results

of this study:

Students. This will help students gather new ideas or

knowledge for future research and possibly related or in

line with this study.

Farmers. This study will help the farmers to produce a

low cost and abundant.

Future Researchers. This study will help them in

searching new ideas about the future research.

 Community. They may have an opportunity to think what

kind of feed they will for a healthy consumption and

effective cultivation.

Definition of Terms

For better understanding of the study, the following

terms were operationally defined.

Azolla Plant. Is a genus of seven species of aquatic ferns

in the family salviniaceae, used in feeds for broiler

chicken.

Commercial Feeds. Commercial feed includes all materials

distributed for use as feed or for mixing in feed for

animals other than man.

Broiler Chicken. Any chicken (Gallus gallus domesticus)

that is bred and raised specifically for meat production.

Weight. A body's relative mass or the quantity of matter

contained by it, giving rise to a downward force; the

heaviness of a person or thing. Used as one of the major

instruments for analysis.

Temperature. The degree or intensity of heat present in a

substance or object, it affects the growth of the boiler

chicken.

Climate. The weather conditions prevailing in an area in

general or over a long period. Which it affect the

condition of the broiler chicken.

Location. A particular place or position. Which the broiler

chicken are being kindered.

Amount. The quantity of the azolla and commercial feed to

feed the broiler chicken (Gallus Domesticus).

Review of Related literature

Azolla Plant

Azolla is used as food supplement for variety of

animals including pigs, rabbits, chickens, ducks and fish.

Azolla is harvested in large quantities and utilized as

fodder for cattle and pigs. It was also found that broilers

feed with Azolla resulted in growth and body weight values

similar to those resulting from the use of maize-soya bean

meal. Azolla slurry remaining after biogas production was

suitable as fish pond fertilizer same levels as with

conventional feeds.

Increase in body weight of the chicks upon feeding

with Azolla was observed. Azolla is a rich source of

protein and essential amino acids and contains several

vitamins such as vitamin-A, vitamin B-12 and beta carotene.

It is also rich in minerals such as Calcium, Phosphorous,

Potassium, Magnesium, Copper and Zinc etc. (Parashuramulu

2013).

Incorporation of Azolla as a feed ingredient in

poultry ration up to 5% has shown growth, high in energy

and high in protein comparable to the birds on normal

ration, and had no deleterious health effect (Basak, 2014).

Our study revealed that the mean average change in the

body weight during 16 weeks with the use of Azolla in the

trial period was 446.95g in Azolla fed group which is

significantly higher than the farmers practice with

supplemented group of backyard poultry bird (Sujatha,

2013).

The use of Azolla in broiler diets be improve the

economic performance of broilers. Azolla is a free floating

fern which can valuable food source. This plant with its
thin roots is like a carpet that has been spread on the

water surface. This plant's leaves are delicate, little and

sensitive and have different colors depending on what point

of time they have grown (Namra, 2010).

Experimental work of showed that Azolla food

supplementation to the ration of broiler chickens improved

feed conversion ratio than control groups and this effect

is due to high weight gain of birds fed Azolla. (Alalade

and Iyayi, 2010).

The Azolla have long been recognized as the cheapest

and most abundant potential source of proteins because of

their ability to synthesize amino acids from a wide range

of virtually unlimited and readily available primary

materials (Fasuyi and Aletor, 2014).

A tropical species of Azolla has reportedly been used

as a feed for pigs and ducks in Indochina; for cattle,

fish, and poultry in Vietnam; and for pigs in Singapore and

Formosa. The North Vietnamese describe Azolla as an

excellent substitute for green forage for cattle and

suggest that it may replace 50% of the rice bran used as

feed for pigs. They also reported that the crude protein of

Azolla was 13% of dry matter and that lysine and tryptophan

were low compared to rice protein. (Thuyet and Tuan, 2016)

 Azolla can be used as an ideal feed substitute for

cattle, fish, pig and poultry, apart from its utility as a

biofertilizer for wetland paddy. It is popular and

cultivated widely in other countries like China, Vietnam,

and Philippines. The production technology has to be

standardized to the diverse and different zones of the

country, to enable its wider spread (Kamalasanana, 2014).

Broiler Chicken

A broiler is any chicken (Gallus gallus domesticus) th

at is grown and raised specifically for the production of m

eat.[1 ] Most commercial broilers achieve slaughter weight

between the ages of four[2 ] and seven weeks, although slow

er increasing breeds achieve slaughter weight at about 4 we

eks.

Basak reported content of Azolla assorted between 3.0

to 3.5%, in current study more or less value was acquired.

The agreement root of CF (Crude Fiber) content of is 4.3%.

(Basak, 2010). Dhumal also observed higher restores in

chicken fed ration where in 5% protein root was substitute

by Azolla. It concluded that dietary replacement of 5%

protein source by Azolla has a good effect on enhancement

in terms of body weight (Dhumal, 2010).

According to Parthasarathy said that feed is the most

expensive of all intakes the product cost is accounted for

feed alone. The poultry nutritionists have been trying to

discover alternative cost productive and progressive feed

ingredients (Parthasarathy, 2010).

The small preparedness in harvesting the benefits and

its capability to take on in all the climates assumption

over the other ventures. In India is closely 30% eggs, 35%

meat is produced by the rural poultry working system. In

evolving countries, the status is unlikely and necessarily

come up to their socio-economic necessity. Generally of the

farmers are poor and nearly 80-85% population is either

landless or small land owner. The status of India is more

or less and the expansion of human population force,

diminishing land resources to urbanization etc.,

monopolistic marketing network and want to make situation

more complex (Rai et al. 2011).

Azolla is historically cultured in the rice-farming

system in China and Southeast Asian countries. The fern is

able to double its biomass in 3–5 days and N input of

110.330 kg N/hm2 per annum has been obtained under optimal

conditions. The importance of Azolla for lowland rice

production has been evaluated in numerous investigations

(Xiaofeng Liu, 2017).

According to Chen under suitable climatic weather,

Azolla undergoes its asexual multiplication by the breakage

of side branches. During vigorous growth in spring or

autumn seasons, its daily multiplication rate reaches as

much as 100 g/m2; annual biomass production is 150–225

t/hm2. Trails proved that its doubling time will be clearly

shortened when grown in artificial controlled environmental

condition,

In addition the above characters of Azolla led us to

evaluate the use of Azolla in CELSS systems. We conducted

the following experiments over the past several years

Generally, Azolla only needs 25–50% full sunlight for its

normal growth; slight shade is of benefit to Azolla growth

in field condition. That is why Chinese farmers culture

Azolla in rice field at early growth stage of rice

seedlings. However, when the light intensity is lower than

1500 lux, the biomass production of Azolla will be greatly

decreased (Liu Chungchu, 2014).

Plant species reported to have medicinal value and

fungicidal properties on fungal genera were collected in

the Philippines and extracted using water. Crude water

extracts of 7 of the 127 plant species screened in vitro

for their fungicidal properties showed inhibitory activity

against Azolla pathogens and Crude garlic extract was found

most effective against Azolla pathogens. Since garlic was

observed to be the most effective plant extract against all

Azolla pathogens, it was given priority in greenhouse tests

to determine protective and therapeutic effects. Results of

the protective and eradicative effects of the garlic

extracts on various Azolla pathogens showed that they have

better therapeutic than protective effect (Lawas, 2015).

Moreover, higher levels of deoxyanthocyanins in Azolla

fronds correlated with feeding deterrence against adult

Lymnea swinhoei snails and Polypedates leucomystax

tadpoles. After 7 days in co-culture, the growth yield of

Azolla filiculoides was almost twice that of Azolla

pinnata, which contained approximately 20-fold higher

levels of deoxyanthocyanins. Inclusion of snails upon

initiating a plant co-culture resulted in an A. pinnata

growth yield that was 1.5-fold higher than that of

filiculoides (Cohen, 2012).

An experiment was carried out to investigate the

effect of dietary inclusion of sun dried and ground Azolla

(Azolla pinnata) on production performance of broiler

chicken. The study was conducted for a period of six weeks

with 160, day-old commercial broiler chicks. The chicks

were divided into four treatments with four replicates of

ten chicks each (Peethambaran, 2015).

There is no clear report available on the nutritional

value of the aquatic fern Azolla, which is capable of

assimilating atmospheric nitrogen efficiently due to the

presence of an algal symbiont in the leaves (Moore, 2016).

Commercial broiler feed in Cameroon is expensive and

therefore out of the reach of small-holder farmers. Maize

and protein concentrates traditionally comprise up to 80%

of farm-made diets. This use of feedstuffs makes the

situation of farm-made diets critical as well. The price of

locally produced maize is increasing. Concentrates

containing animal proteins used to be imported. However, as

from February 2001 the use of meat meals in livestock

feeds, including broilers, has been banned in Cameroon.

Thus, feedstuffs alternative to maize and meat meals should

be sought. Small-holder farmers control up to 72% of the

national broiler production (Djoukam and Teguia 2010),

 The efficiency of their production is low. It is

necessary to look for locally available, cheap, safe and

nutritionally adequate substitutes for maize and meat meals

in broiler feeds. Identification of such feedstuffs would

help resource-poor farmers not only to cut down their

production costs, but also to improve the efficiency of

their production (Teguia and Beynen 2013).

The broiler production industry has, for many years,

continuously improved animal performance, reflected by ever

decreasing feed conversion (kg feed consumed per kg body

weight) and reduced time to achieve market weight (Zuidhof

et al., 2014).

Different feed ingredients are used in poultry diets

in different regions around the world, with wheat and corn

the most predominant cereals, and soya the most important

protein source. Substituting (partly) these high‐quality

feed ingredients with alternative feed materials, often by‐

products from other industries or local products, could be

a valuable way to further enhance sustainability of poultry

production worldwide. Such alternative feed sources are

often cheaper but may be less digestible, so animal

performance can be reduced and the incidence of gut health

problems can increase (Ravindran, 2013).

 In commercial poultry, the production of broiler feed

contributes up to 70% of the total production cost. Due to

increases in global feed prices, there is now a tendency in

the poultry industry to move towards alternative or

unconventional feed ingredients. This move is however

limited by several issues: high and low fibre and protein

contents and the presences of antinutritional factors (ANF)

in unconventional feed ingredients that can reduce feed

digestibility. Previous studies have shown that

fermentation increased crude protein content but decreased

crude fibre content , several ANF and toxic compounds in

feed ingredients (Sugiharto et al., 2015a).

Nowadays, various commercial feed mills are producing

different forms of broiler feed for different age group of

bird. Pelleting is a processing method that is employed by

the feed manufactures to improve farm animal performance.

The physical form of feed (mash, pellet and crumble) is a

crucial factor in meat yield of broiler. Different types of

feed forms have been evolved in broiler production at the

present time. Various feed forms pellet, mash or crumble

that to be supplied to broiler are the most important

factor which directly influence the cost of mixed feed and

production performance of broiler. The major objective of

poultry feeding is the conversion of feedstuff into human

food. Mash is a form of a complete feed that is finely

ground and mixed so that birds cannot easily separate out

ingredients; each mouthful provides a well balanced diet.

However, ground feed is not so palatable and does not

retain their nutritive value so well as ungrounded feed

(Mendes et al., 2011).

Broilers were fed the six types of feed during the

starter phase between 0-5 weeks of age and during the

finisher phase between 5-9weeks. Results showed that

although one of the commercial feeds outperformed all other

feeds in terms of weight gain and feed efficiency, the

overall growth performance on on-farm feeds were comparable

to those on commercial feeds even where one of the on-farm

feeds contained sub-optimal levels of energy. However, feed

cost kg weight gain was significantly lower for on-farm

feeds (Kehinde, 2014).

Feeds

Many Commercial poultry farms had collapse while a

good number of them experienced slow growth as a result of

sudden increases in the cost of poultry feeds (Ogundipe,

2012 and Onimisi, 2014). However, farmers may not have a

direct influence on the feed cost when feed is purchased

from the commercial Feed Millers. Many farmers are looking

for an alternative ways of boosting their profits, many

farmers change from one commercial feed to another in

search of a better feed (Ogundipe et-al.1986)

While a good number have decided to be producing their

own feeds. as a matter of fact, feed cost as well as the

quality of the feeds are among the factors which dictate

farmers preference for commercial or self- compounded feeds

(Umeh and Odo 2012).

Conducted an experiment to study the effect of three

levels of Fe (20, 40 80 mg/kg), among other minerals on the

broiler performance and tissue trace mineral contents. The

diets organically supplemented with 40 mg/kg Fe achieved a

superior feed conversion and lower mineral excretion than

the inorganic control. The other trace minerals had similar

effects at specific levels. So organically complexed Cu,

Fe, Mn and Zn appeared to meet broiler requirements at

lower levels than inorganic supplements and did not

compromise broiler growth (Bao et al , 2013).

Determined the effects of iron sulphate

supplementation of diet containing 15% cottonseed meal on

the live weight gain, feed consumption, feed efficiency and

some haematological parameters of broilers. At the end of

the 4 weeks experiment, mean live body weights in Fe group

was significantly greater than that of the control group.

The values of feed conversion ratio of control and Fe

groups were 2.72 and 2.40, respectively (Oguz et al, 2011).

In this study, 183 livestock feeds and 85 animal

manure samples were collected from commercial farms in

England and Wales and analysed to determine their heavy

metal (zinc, copper, nickel, lead, cadmium, arsenic,

chromium and mercury) contents. Zinc and copper

concentrations ranged from 150±2920 mg Zn/kg dm (dry

matter) and 18±217 mg Cu/kg dm in pig feeds, depending on

the age of the pigs. In poultry feeds, concentrations

ranged from 28±4030 mg Zn/kg dm and 5±234 mg Cu/kg dm, with

laying hen feeds generally having higher heavy metal

contents than broiler feeds. Concentrations of Zn and Cu in

dairy and beef cattle feeds were much lower than in pig and

poultry feeds. Pig manures typically contained c.500 mg

Zn/kg dm and c.360 mg Cu/kg dm, re¯ecting metal

concentrations in the feeds. Typical concentrations in

poultry manures were c.400 mg Zn/kg dm and c.80 mg Cu/kg

dm, and in cattle manures c.180 mg Zn/kg dm and c.50 mg

Cu/kg dm. The dry matter content of cattle and pig slurries
was a useful indicator of heavy metal concentrations on a

fresh weight basis (Nicholson , 2015).

The basic requirement for a feed fraction to have an

effect on digestibility is for its content to be correlated

with its digestible quantity in the diet. This is the so-

called Lucas test and is an essential component of modeling

studies. Further, for any component to have a consistent

effect upon digestibility it must have a true causative

effect. Lignin is such a component, but its effect is

limited to the plant cell wall, which is in turn another

variable. These points are the foundation of the summative

equation for estimating digestibility (Van Soest and Jones

2015).
 Chapter 2

METHODOLOGY

Research Design

In this study, Two-Group experimental design will be

used for the effectiveness of azolla as a feeds for broiler

chicken. According to Michael (2015) a two-group design is

a kind of research format that posttest-only randomized

experiment. It has two lines -- one for each team -- with

an R at the starting of each line to indicate that

the businesses had been randomly assigned.

One group gets the cure or program (the X) and

the different group is the evaluation team and doesn't get

the program. This study aims to determine the effectiveness

of the experimental group known as to be a Azolla (Azolla

pinnata) as an alternative feed for broiler chicken (Gallus

Domesticus). Thus, two-group research design will be

considered suitable for this study.

Two-group design between the control group (commercial

chicken feeds) and experimental group (Azolla plant). The

data that will be gathered between the two is determined by

recording the weight (kilograms) of Broiler Chicken(Gallus

Domesticus) at time intervals of 15 days and 30 days.

 Table 1. Control and experimental group data observation

Yield

Commercial Chicken feeds Azolla Plant(Azolla pinnata)

Poultry
(Control) (Experimental)

0 days 15 days 30 days 0 days 15 days 30 days

..

10

Statistical treatment

The statistical tools to be used in analyzing,

interpreting of data and testing the null hypotheses of the

proposed study are the mean and t-test.

Weighted mean will be used to calculate the average of

the gathered result. The formula for the weighted mean is:

𝑥𝑖
𝑥̅ = ∑
𝑛

Where, 𝑥𝑖 is the measurement, and 𝑛 is the number of

measurements.
 After getting the weighted mean, t-test will be used

to determine the significant difference in the

effectiveness of Azolla (Azolla pinnata) as an alternative

feeds for boiler chicken (Gallus Domesticus). According to

Ostertag (2013) analysis of variance (ANOVA) is a

statistical treatment concerned with comparing means of

several samples to assess if mean differences exist on one

continuous dependent variable by an independent variable.

The formula for the t-test as follows:

(𝑥̅1 − 𝑥2 ) − (𝜇1 − 𝜇2 )
𝑡0 =
𝑠2 𝑠2
√ 1+ 2
𝑛1 𝑛2

The level of significance that will be used in the

study is 5% or 95% level of confidence to determine the

significance of the findings.

PROCEDURE

Gathering of Azolla Plant

A. Azolla Plant

Azolla Plant will be freshly taken in Barangay

Dahican, Magallanes. Approximately two (2) sacks of Azolla

plant using.

Making Poultry House

1. Cut piece of wood past 2x2x18 inches

18 inches

2 inches

2. Cut 2 pieces of 2x2x36 piece of wood for the vertical

base.

2 inches

36 inches
3. Join the past and vertical base together and nail
4. Cut 10x36 inches of screen for the floor
5. Buy 1 Pack of bamboo wood and nail for side walling

6. Put a light inside of the poultry house

7. Place a fly wood under the screen

Preparation of Azolla Broiler Chicken Feeds

Before the Azolla broiler chicken feeds is going

to be applied to the broiler chicken. First, The Azolla

plant was ground. Then, The dried Azolla is mixed with

commercial feeds with the ratio of 1:1 with 40 grams. It

measured using a weighing scale.

Application of Azolla broiler chicken feeds

The Broiler chicken(Gallus Domesticus) will be

placed on the separated poultry house which is labelled by

the controlled or experimental variable (Each poultry house

must consist of 10 Broiler chicken (Gallus Domesticus) and

will be feed two times a day between the hours of 6-7 am,

and 5-6 pm with the amount of 80 grams of Azolla broiler

chicken feeds each chick per day.

Data Analysis

The obtained result were used to compare the Azolla

broiler chicken feeds and the Commercial broiler chicken

feeds , Mean and t-test will be analyzed using the Data

analysis of Microsoft Excel.

Illustration of Poultry House

Control-G Treatment-G (Experimental)

Control Treatment
group group
(Experimental)

C1 T1

C2 T2

C3 T3

C… T…

C10 T10

Commercial Azolla
Broiler Broiler
chicken feeds chicken feeds
Flowchart Diagram of the Procedure

Collection of Azolla Plant

Making of Poultry House

Preparation of the Azolla

broiler chicken feeds

Application of Azolla

broiler chicken feeds.

Data Analysis

Figure 2. The Flowchart of the Procedure