q CESCO a IN PULP & PAI RECYCLING MILL Cesco E-200 esterase enzyme is used in liquid form to hydrolyze stickies at various pulp and papei recycling mills, CASE A pulp & paper recycling mill producing 90 to cardboard (OCC), applied the Ci per day of package paper grades, using old corrugated se composition for stickies hydrolysis. ‘The product was dosed into the Pulper at 40 milliliters per ton OCC. Graph No. 3 shows average monthly values of micro- stickies measured at the wire pit, The red bar indicates the average base line value (not using Cesco E-200 sterase). Micro-stickies counts were made by using the hemocytometer method and units are measured a millions of micro-stickies per milliliter ‘The data shows that the Cesco E-200 esterase product produced a removal efficieney of 74% micro- stickies by hydrolysis in the paper manufacturing process. Graph 1 - EFFECT OF CESCO E-200 ENZYME ON OCC STICKIES HYDROLYSIS. CASE2 A pulp & paper recycling mill, producing 140 tons per day of package paper grades using OCC as raw ‘material, began the application of Cesco E-200 esterase for stickies hydrolysis. The product was dosed into the Pulper at a rate of 40 milliliters per ton of recycled paper. Graph No. 2 shows values of micro- stickies counts measured at the head box before and during the product application. The red bars indicate the base line values (before using Cesco E-200 esterase). Micro-stickies counts were determined using the hemocytometer method and units are measured as millions of micto-stickies per milliliter. ters /Production 15 frongate Road, Bellingham WA 98226 duction Facility: 5623 Barkaloo R own 1K 7752‘The data show that Cesco B-200 esterase provided a micro-stickies removal etic hydrolysis in the paper manufacturing process. HYDROLYs GRAPH 2- EFFECT OF CESCO E-200 ENZYME ON STICKIES 0.00 0.00 20.00 20.00 millions/mt. 10.00 acca eee eee 3 eer aeee a 2 2 2 2 2 2 g 8 2 4a cues a g a Peet a Date ‘A paper mill producing 40 tons per day of tissue paper grades using recycled paper as raw material began using Cesco B-200 esterase for stickies hydrolysis due to low converting productivity caused by continuous paper breaks. The esterase was dosed into the Pulper at a rate of 40 milliliters per ton of recycled furnish, The mill was able to eliminate converting paper breaks and increase productivity. Normal converting production without using the Cesco E-200 esterase was 14 tons/day. Two days after beginning to use the esterase, the mill was able to increase production to 19 tons/day. After three days. using the Cesco E-200 esterase formulation, converting productivity reached 24 tons/day (70% improvement). CASE4 A paper mill producing 70 tons per day of tissue paper grades using recycled paper applied Cesco E-200 esterase for stickies hydrolysis. The product was added to the Pulper at a rate of 40 milliliters per ton of furnish. Graph No.3 shows macro-stickies counts at different points in the process (left to right is downstream direction). The graph demonstrates how macro-stickies are removed along the process by the hydrolytic action of Cesco E-200 esterase. Macro-stickies counts were made by using the hemocytometer method and the units are # of macro-stickies per milliliter. The data shows that the esterase composition removed 97% of macro-stickies along the process.GRAPH 3 - EFFECT OF CESCO E-200 ENZYME ON MACRO. AT UNIT OPERATION SAMPLE POINTS IN A PAPER MANUFACTURING PROCESS. KIES. 20 200 | Removal of Macro-stickies ‘along the process is, 97% Macrostikies per ml so | PROCESS EQUIPMENT- DOWNSTREAM DIRECTION > a Homogenizer Collin Cellout Tower Tower?——Head Box ‘The following photomicrographs show macro-stickies density in the hemocytometer at the initial process sample point (homogenizer) and at the final process sample point (head box). ‘There is a dramatic difference in the macto-stickies count between the homogenizer and headbox sample points, supporting, the data presented in Graph 3 and supporting the performance of the Cesco E-200 esterase in stickies, hydrolysis HOMOGENIZER HEADBOX |