ANALYTICAL CHEMISTRY

ITS IMPORTANCE IN THE BIOLOGICAL SCIENCES

What is Cholesterol? What is
Cholesterol Testing?
-Cholesterol is a soft waxy, fat-like substance -Cholesterol test is simply a blood test
-maintains the structural integrity and fluidity of cell -Also known as lipid panel or lipid profile
membranes -Measures four types of lipids or fats in the blood:
-”Base Steroid” Total cholesterol, Low-density lipoprotein (LDL),
-Participates in the biosynthesis of bile acids in the liver High-density lipoprotein (HDL), and triglycerides
-LDL and HDL -VAP Cholesterol Testing
Why test for cholesterol?
-Cholesterol has a bad reputation due to its
association with cardiovascular diseases.
-Evaluates a person’s risk with CHD
-As of 2016, Department of Science and
Technology ’ s Food and Nutrition Research
Institute (DOST-FNRI) reported that one in two
Filipino adults faces borderline to high-
cholesterol levels
-46.9 percent Filipinos aged 20 and above at
increased risk of cardiovascular diseases
Gas Chromatography- Mass Spectrometry
-ideal for the analysis of the hundreds of relatively low molecular weight compounds
found in environmental materials
-used for routine cholesterol determination
-used to separate cholesterol from other interfering species
-used for determination of free, esterified and total cholesterol levels (based on
sample pretreatment)
-More accurate than classical chemical and enzymatic methods
 Liquid Chromatography-
Mass Spectrometry
-capable of analyzing a much wider range of
components
-used for routine cholesterol determination
-used to separate cholesterol from other interfering
species
-used to separate cholesterol from other interfering
species
-More accurate than classical chemical and
enzymatic methods
 MALDI-IM- IMS
-ideal for the analysis of the hundreds -Matrix-assisted laser
of relatively low molecular weight desorption/ionization-ion-mobility
compounds found in environmental mass spectrometry
materials -coat samples with metal, which
-using sputtered silver nanoparticles increases the conductivity of insulating
instead of matrix substrates, such as tissues or cells.
 REFERENCES
• Alarming cholesterol levels among Filipinos. (2016, August 3). Retrieved November 20, 2018, from
Manila Standard Lifestyle: http://www.manilastandard.net/lifestyle/wellness-
environment/212293/alarming-cholesterol-levels-among-filipinos.html
• Bull, I. D. (2005, April 27). High Performance Liquid Chromatography Mass Spectrometry (HPLC/MS).
Retrieved November 22, 2018, from University of Bristol:
http://www.bris.ac.uk/nerclsmsf/techniques/hplcms.html
• Bull, I. D. (2008, January 14). Gas Chromatography Mass Spectrometry (GC/MS). Retrieved
November 20, 2018, from University of Bristol: http://www.bris.ac.uk/nerclsmsf/techniques/gcms.html
• Cholesterol Test. (2018). Retrieved November 20, 2018, from healthline:
https://www.healthline.com/health/cholesterol-test
• Cholesterol Testing. (2015, June 18). Retrieved November 20, 2018, from Virtual Medicine Testing:
https://www.myvmc.com/investigations/cholesterol-testing/
• Li, L.-H., Dutkiewicz, E. P., Huang, Y.-C., Zhou, H.-B., & Hsu, C.-C. (2018, September 1). Analytical
methods for cholesterol quantification. Retrieved November 20, 2018, from Journal of Food and Drug
Analysis: https://www.jfda-online.com/article/S1021-9498(18)30146-7/fulltext#sec2.1
• Mocon-Ciriaco, C. (2016, July 13). Cholesterol levels of Pinoys rising–survey. Retrieved November 20,
2018, from BusinessMirror: https://businessmirror.com.ph/cholesterol-levels-of-pinoys-rising-survey/
• Simon, E. J., Dickey, J. L., Hogan, K. A., & Reece, J. B. (2016). Campbell Essential Biology with
Physiology (Fifth ed.). Harlow, Essex, England: Pearson Education Limited.