Beruflich Dokumente
Kultur Dokumente
SL/Lab Manual/WM/2018
2018.11.10
1.0 Introduction
Laboratory waste Management Guideline provide procedural information for laboratory workers to
follow when disposing of waste generated in laboratories in order to minimize risks associated with
the disposal of laboratory waste. These guidelines apply to all workers, and visitors who work within a
laboratory environment
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1.2 Mixed Hazardous Waste
Waste that contains multiple hazards is classified as “co-mingled”. Co-mingled hazardous waste
should be disposed in a manner that best addresses ALL hazards. Segregation of the same type of
waste contaminated with different hazards may be necessary.
Eg: contaminated sharps into separate, labelled containers to segregate chemical and biological
contamination. Never mix incompatible waste in the one bin or bag.
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2.2 Chemicals Waste
Bin colour: yellow base with orange lid
Final disposal method: incineration
Waste chemicals can take various forms including solvents, aqueous solutions, dry powders, and
unwanted old chemicals. The SDS for each chemical must be checked to ensure compatibility of
materials for mixed chemical residue containers. Where possible, mixing of chemicals should be
avoided to prevent unexpected reactions from occurring. A bulging waste container must be dealt with
immediately.
The waste container should be compatible with the residue material placed within. If the waste is a
liquid, residue containers are approved strong, plastic sealable containers. Only containers up to 5
litres can be accepted by the Waste Store. Containers up to 20L can be collected from their location ( a
Waste Tracking Log is to be taken to the Waste Store in place of the container). No hazardous
chemical substances should be disposed down drains. Generally chemical waste should be segregated
according to its properties, such as:
Aqueous acidic
Aqueous alkaline
Halogenated
Non-halogenated
General hazardous waste – powders etc.
Toxic
Cytotoxic
Completed Hazardous Waste labels should be put to the hazardous waste residue container. If a
chemical reagent bottle has lost its label and the identity of the substance is unknown, label with
“Caution unknown substance - Do not use”. These bottles can be taken directly to Waste Store for pick
up.
Hazardous waste is collection day and time should be defined at the Waste Store and Store opening
dates should be listed and sent to each department.
A Waste Tracking Log is required when leaving waste at the Waste Store. It is advisable for each
laboratory to keep a copy of the Waste Tracking Log.
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2.3 Cytotoxic Waste
Cytotoxic waste is any substance contaminated with any residue or preparations that contain materials
that are toxic to cells principally by their action on cell reproduction. All cytotoxic waste (class 6.1)
should be placed in an approved purple cytotoxic bag or container. When the residue container is full,
place in purple labelled cytotoxic waste wheelie bin kept in secure area. Although the final disposal
method for cytotoxic waste is the same as chemically contaminated waste, it must be treated more
securely prior to incineration due its mutagenic potential.
Liquid Waste
Eg: buffer solutions, stock solutions, discarded disinfectants, discarded Formalin, infected secretions,
aspirated body fluids, liquid from laboratories and floor washings, cleaning, house-keeping and
disinfecting activities, silver X Ray developing liquid etc
Keep record of amount of EtBr in solution
Place in spill proof container with bunding or secondary containment
Apply Cytotoxic Waste Disposal Identification Label (and label for any secondary hazards)
Dispose via Hazardous Waste Store
The chemical liquid waste of the hospital must be collected through a separate drainage system
leading to an Effluent Treatment Plant (ETP). Hospitals with large laboratory shouldl install ETP for
separate collection and disinfection of infectious waste from laboratory prior to mixing the same with
rest of the wastewater from hospital for further treatment. For middle and small healthcare facilities
having no system of separate ETP the liquid waste is needed to be onsite chemically disinfected with
chlorine solution in a tank before mixing the same with other wastewater.
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Solid Waste
Eg: gels, powder, contaminated consumables (eg gloves, paper, used tea bags, etc)
Place waste in laboratory bin lined with purple cytotoxic bag
If deemed is necessary, first put waste in sealed bag/container to avoid unnecessary exposure
or contact
When bin/bag is full, place in larger secure purple base/purple lid cytotoxic bin which is in a
secure location
Final disposal method: autoclave then landfill. Disposal of the anatomical waste in the deep burial pit
should not be practiced unless the hospitals is located in remote isolated place. Use of deep burial pit
should be as authorised by the respective officer. This waste should be disposed through twin
chambered compact incinerator with 2 seconds retention time in secondary combustion chamber and
air pollution control devices as specified. Items contaminated with blood, body fluids like dressings,
plaster casts, cotton swabs (soiled waste) should be disposed through twin chambered compact
incinerator with 2 seconds retention time in secondary combustion chamber and air pollution control
devices as specified. Soiled waste can also be disposed in captive deep burial pits only in case of the
hospitals located in remote isolated place. Use of deep burial pit should be as authorized.
All the microbiological waste, blood bags, cultures, dishes and other highly infectious waste is needed
to be pre-treated by disinfection up to microbial kill before handing over the waste. Pre-treatment
should be done by autoclave / microwave / hydroclave. Disinfection can also be adopted as pre-
treatment method by using non-chlorinated chemical disinfectants like calcium oxide, phenolic
compounds etc. The pre-treated waste bags should be handed over on daily basis. Incineration in twin
chambered compact incinerator with 2 seconds retention time in secondary combustion chamber and
air pollution control devices Pre-treated waste can be disposed in captive deep burial pits in case of the
hospitals located in remote isolated place. Use of deep burial pit should be as authorized.
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2.5 Sharps Waste
Sharps are objects or devices that have acute, rigid corners, edges, points or protuberances capable of
cutting or penetrating the skin e.g. hypodermic needles, broken glass, scalpel blades, lancets, syringes
with needles, razor-blades.
Final disposal method: Dependent upon primary contamination. Dispose of the sharp after
chemical disinfection and dispose in captive concrete waste sharp pit. Alternatively treat by
Autoclaving or Dry Heat Sterilization followed by shredding or mutilation or encapsulation in
metal container; combination of shredding cum autoclaving prior to disposal in sharp pit and send
to iron foundries
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2.6 Glass Waste
Bin colour: green base with brown lid (brown glass)
green base with white lid (clear glass)
Final disposal method: recycling after autoclaving or Dispose of in sharp pits.
Glass, whether broken or unbroken, should not be placed in general waste bins. The bottle cap can be
removed and disposed in the general waste bin. Once clean, place glass in waste bin based on glass
colour. When the laboratory glass bin is ¾ full, the lid should be placed on the bin and the contents
transferred to the larger solid waste bins.
Broken Glass
Broken glass should be treated as Sharps waste. If pieces of broken glass are too large for a sharps
container, they should be placed into an impervious container with a secure lid, and then placed in
appropriate wheelie bin.
Contaminated Glass
Any glass that has been contaminated, and unable to be safely decontaminated, should be treated as
other waste of the same hazard eg: Chemically Contaminated Waste Biological/Clinical, GMO and
Biosecurity Waste, cytotoxic. Contaminated glass containers or laboratory glass such as beakers,
volumetric flasks of other Pyrex items cannot be placed in general recycling bins.
Radioactive waste should be packaged according to its primary hazard eg: Chemically Contaminated
Waste or Biological/Clinical, GMO and Biosecurity Waste. It will be kept in the Radioactive Waste
Store to “delay and decay” prior to final disposal as non-radioactive waste.
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3.0 General Waste
General waste consists of all the waste which has not been in contact with any hazardous or infectious,
chemical or biological secretions and does not include any waste sharps. This waste consists of mainly
the papers, cardboards, food, textile, general discharge, , C&D wastes, horticulture wastes, etc. These
general wastes are further classified as dry wastes and wet wastes and should be collected separately.
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Hazardous Waste should be segregated at the point of generation by the person who is
generating the waste in designated colour coded bin/ container
Hazardous Waste and General Waste shall not be mixed. Hazardous Waste and General Waste
shall not be mixed. Storage time of waste should be as less as possible so that waste storage,
transportation and disposal is done within 48 hours.
Chlorinated plastic bags for collection of biomedical waste should not be used. All efforts
should be made to minimize the chlorinated plastics in Hazardous waste.
Secondary handling of waste should not be done at healthcare facility.
Only Laboratory and Highly infectious waste should be pre-treated onsite before sending
for
final treatment or disposal.
All bags or containers containing segregated Hazardous waste should be labelled before such
waste goes for final disposal.
Responsibility of various categories of staff in regards to BMW Management is given in the
Table 01.
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4.1 Hazardous waste Segregation
It is required segregate waste at the point of generation as per the colour coding mentioned above
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3.2 Packaging
Hazardous waste bags and sharps containers should be filled to no more than three
quarters full. Once this level is reached, they should be sealed ready for collection.
Plastic bags should never be stapled but may be tied or sealed with a plastic tag or tie.
Replacement bags or containers should be available at each waste-collection location so
that full ones can immediately be replaced.
Colour coded waste bags and containers should be printed with the bio-hazard symbol,
labelled with details such as date, type of waste, waste quantity, senders name and
receivers details as well as bar coded label to allow them to be tracked till final
disposal.
Ensure that Bar coded stickers are pasted on each bag as per the guidelines
3.3 Labeling
All the bags/ containers/ bins used for collection and storage of hazardous waste, must be labelled with
the warning Symbol of Bio Hazard or Cytotoxic Hazard as the case may be as per the type of waste
All the bags and containers to be transported must also be labeled with following details:
Date of Generation
Type of waste category
Waste Quantity in kg
Name and Address of the hospital
Contact Person Name and Phone Number
Contact Details in case of any Emergency
Receivers contact details ( Name, Address and Contact Details)
Please Refer to Annexure: Label for Hazardous Waste Containers & Bags
Please refer to Annexure: Label for Transporting of hazardous Waste and Containers
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Figure 04: Label for collected Waste Bag
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Figure 07: Examples of Waste Collection Trolleys for Transportation:
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Such center must be roofed and manned and is under lock and key under the responsibility
of designated person.
The entrance of this center must be accessible through a concrete ramp for easy
transportation of waste collection trolleys.
Flooring should be of tiles or any other glazed material with slope so as to ease the cleaning
of the area.
During construction it is to be ensured that the center is kept ventilated through the use of
exhaust fan or by use of wire meshes for ventilation.
It is to be ensured by the health care facility that such central storage station is safety
inspected for potential fire hazard and based on such inspection preventive measure has to
be taken by the health care facility like installation of fire extinguisher, smoke detector etc.
There should also be provision for water supply adjacent to central waste storage area for
cleaning and washing of this station and the containers. The drainage from the storage and
washing area should be routed to the Effluent Treatment Plant.
Sign boards indicating relevant details such as contact person and the telephone number
should be provided.
The entrance of this station must be labeled with “ENTRY FOR AUTHORIZED PERSONAL
ONLY” and Logo of hazardous Waste Hazard.
It is to be ensured that no general waste is stored in the central waste collection area.
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6.0 Handing over the Hazardous Waste
6.1 Minimum Requirements
Following Steps are needed to be undertaken by Health Care Facility while handing over the bio
medical waste.
All the hazardous waste should be collected in the colour coded bags/ bins/ containers and
should not be mixed with the general waste generated by the health care facility.
All the bags or containers containing bio- medical waste, to be sent out of the premises must
also be labeled as per BMWM Rules and also with the unique bar code.
Collect or receive a receipt generated from bar-code scanning system.
The laboratory must ensure that there is no secondary handling of the waste (the waste is
handed over directly from laboratory to central waste collection center).
Each bag should be labelled with barcode for identification of waste and ensure that the details
of weight and time of collection
All the bags/ containers used for collection and storage of hazardous waste must be labeled as
described earlier.
It is the responsibility to ensure that any untreated anatomical waste; soiled waste should not
be stored in the health care facility's waste collection area beyond a period 48 Hours.
If the waste collection agency does not collect the waste within agreed time, which must not
exceed beyond 48 hrs, it is the responsibility to immediately notify to the prescribing authority
about any such lapse.
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Management Responsibility
Each healthcare facility has to perform certain roles and responsibility in order to successfully
implement the Bio Medical Waste Management process.
Handling of waste through waste segregation, collection, its pre-treatment and storage
activities ensuring protection of environment and human health from any adverse effect, which
laboratory.
There should be a person having administrative control on waste management in the
laboratory.
The In charge of the laboratory should (Define with designation) take all necessary steps to
ensure that bio-medical waste is handled without any adverse effect to human health and the
environment and in accordance with environmental authority ensuring properly segregated,
handled, stored, packaged, transported and disposed of, as per these guidelines to ensure
successful implementation of hazardous waste management.
Ensure that all the legal requirements related to the Bio Medical Waste Management are
complied with and are regularly updated
Ensure that annual reports and accidents reports are submitted in a timely manner.
Ensure that bio-medical waste is handled without any adverse effect to human health and the
environment.
Make a provision within the premises for a safe, ventilated and secured location for storage of
segregated biomedical waste
Ensure that there shall be no secondary handling, pilferage of recyclables or inadvertent
scattering or spillage by animals
Ensure that bo-medical waste from central waste collection storage or premises shall be
directly transported to the common bio-medical waste treatment facility for the appropriate
treatment and disposal
Ensure pre-treatment of all the laboratory waste, microbiological waste, blood samples and
blood bags before handling to over to for final disposal.
Ensure that the solid waste other than BMW is disposed of as per relevant rules and laws and
there is no mixing of hazardous/biomedical and solid waste
Ensure all the staffs of HCFs are provided regular training on BMW handling both at the time
of induction and on annual basis as well
Ensure occupational safety of all the employees through annual health checkups, immunization
and provisions of appropriate and adequate PPEs
Ensure that biomedical/hazardous waste Register is maintained & updated on day to day basis.
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Ensure that monthly and annual records of the waste generated from the facility is
uploaded
on its own website as well as on State Pollution Control Website(SPCB)
Immediately inform the SPCB in case of any lapse by waste collection agency in collection of
waste.
Ensure that all the activities of waste management are monitored and reviewed
Ensure that the committee formed for monitoring and review of BMW management is
functioning properly.
Ensure that all the records related to BMW Management are maintained.
Ensure that all the requirements related to establishment of a treatment facility within its
premises are fully complied with
All the listed responsibilities are detailed in these guidelines,
laying down all the steps which are needed to be undertaken by health care facility in order to
fulfill these responsibilities.
Establish an accident reporting system
Record maintenance and define record retention period
Ensure Occupational safety
o Providing adequate and appropriate Personal Protective Equipment (PPE) to the staff
handling Bio Medical Waste
o Conducting health check-up of all the employees at the time of induction and also at
least once in a year.
o Ensure that all the staff of the health care facility involved in handling of BMW is
immunized at least against the Hepatitis B and Tetanus.
o Taking remedial steps in accordance to any accident occurred.
o Ensure wearing PPE by all health care workers
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Budget Allocation for Waste Management
Ministry of health should allocate annual budget foe waste management implementation as per the
requirement of hospital laboratories and maintain the un interrupted waste management process
Following completion of any review, the program will be revised and, if necessary, updated in order to
correct any deficiencies.
Related Documents
Biosafety Manual
Hazardous Waste Disposal Flowchart
Hazardous Waste Disposal Guidelines
Hazardous Waste Management Contact List
Radioactive Waste Disposal Guidelines
Working with Sharps Guidelines
Laboratory Waste Disposal SOPs
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Annexure: Preparation of Sodium Hypochlorite solutions
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