3.

RESULT AND DISCUSSION

After four successful readings for each sample of raw, boiled and microwaved potatoes, using

the Kjeldahl’s method were complete, the nitrogen content, and in turn, the protein content was

calculated. First, the moles of ammonia were deduced, by subtracting the moles of hydrochloric

acid and the moles of sodium hydroxide. The number of moles of ammonia were then multiplied

by the molar mass of nitrogen, i.e. 14.00671, to determine the mass of nitrogen present in the

sample. To calculate the percentage of nitrogen in the sample, the mass of nitrogen, determined

in the previous step, was divided by the original weight of the potato sample and multiplied by

100. The nitrogen percentage calculated was multiplied by 6.25 because protein is 16% nitrogen

(100÷16=6.25). Thus, the formula used to calculate the protein content is-

(𝑚𝑜𝑙𝑒𝑠 𝑜𝑓 𝑎𝑐𝑖𝑑 − 𝑚𝑜𝑙𝑒𝑠 𝑜𝑓 𝑏𝑎𝑠𝑒) × 14.0067 × 100 Commented [SC1]: Where is this 6.25 from? I still
Percentage of protein= 6.25 × 𝑤𝑒𝑖𝑔ℎ𝑡 𝑜𝑓 𝑡ℎ𝑒 𝑠𝑎𝑚𝑝𝑙𝑒 don’t know.
Commented [SJ2R1]: Specified maam
Calculation for the first sample of raw potato- Commented [SC3]: What do these boxes denote??

((0.015 ±0.5)−(0.012 ±0.5)) × 14.0067 × 100 Commented [SJ4R3]: The boxes come automatically
6.25 × = 35.89 ±0. 36 when I want to insert an equation.
1.02 ±0.01
Commented [SC5]: You must explain to me how each
digit came to be.
The calculated percentage of protein values for the remaining samples of potatoes are shown in Commented [SJ6R5]: Ive explained the formula now
maam.
the table below- Commented [SC7]: Please get this page printed and
checked (signed) by Ms. Vandana in order for me to
approve it.
Raw/% [±0. 36] Microwaved/% [±0. 31] Boiled/% [±0. 27]
Commented [SC8]: Please get this page printed and
checked (signed) by Ms. Vandana in order for me to
1. 35.89 31.51 27.06 approve it.
Commented [SC9]: Please get this page printed and
2. 35.02 29.88 26.83 checked (signed) by Ms. Vandana in order for me to
approve it.
3. 34.14 31.63 26.47
Commented [SC10]: Please get this page printed and
checked (signed) by Ms. Vandana in order for me to
4. 34.33 29.79 27.13 approve it.

Table 1. Percentage of protein content in the 3 samples of potatoes.

1
"Nitrogen - Atomic Number - Atomic Mass - Density of Nitrogen" 2018