PMID- 31453123

OWN - NLM
STAT- PubMed-not-MEDLINE
LR - 20190829
IS - 2225-4110 (Print)
IS - 2225-4110 (Linking)
VI - 9
IP - 4
DP - 2019 Oct
TI - Antioxidant properties and membrane stabilization effects of methanol extract
of
Mucuna pruriens leaves on normal and sickle erythrocytes.
PG - 278-284
LID - 10.1016/j.jtcme.2017.08.002 [doi]
AB - This study was aimed at investigating the antioxidant properties and membrane
stabilization effects of Mucuna pruriens leaves on sickle erythrocyte as a
possible means of sickle cell disease management. Pulverized plant material
was
extracted with methanol, filtered and concentrated at reduced pressure with a
rotary evaporator. Phytochemical analysis and antioxidant studies of the
extract
were carried out using standard methods. Blood samples of volunteer sickle
cell
patients and healthy individuals used in the study were collected from the
University of Nigeria Medical Centre and University campus community, Nsukka
respectively. The genotypes of the individuals were confirmed by cellulose
acetate paper electrophoresis. Water induced haemolysis of human red blood
cell
was used to assess membrane stabilization of the erythrocytes. Phytochemical
result of the extract showed the presence of alkaloids, flavonoids, tannins,
proteins, terpenoids, saponins, cardiac glycosides and anthraquinones.
Antioxidant vitamins C and E were present in concentrations of 495.36 mg/100
g
and 101.03 mg/100 g respectively. The percentage (%) scavenging activity of
the
1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and hydroxyl radical by the
extract
was significant. The extract exhibited membrane stabilization on both normal
and
sickle erythrocytes. The percentage (%) inhibition of haemolysis by the
extract
in both normal and sickle erythrocytes at different concentrations of 100,
200,
400, 600 and 800 mug/ml were significant and concentration dependent. We
conclude
that M. pruriens leaves have antioxidant properties and erythrocyte membrane
stabilizing potentials and could be recommended for use in the management of
patients with sickle cell anaemia.
FAU - Anosike, Chioma Assumpta
AU - Anosike CA
AD - Department of Biochemistry, University of Nigeria, Nsukka, Enugu State,
Nigeria.
FAU - Igboegwu, Odinaka Ngozi
AU - Igboegwu ON
AD - Department of Biochemistry, University of Nigeria, Nsukka, Enugu State,
Nigeria.
FAU - Nwodo, Okwesilieze Fred Chiletugo
AU - Nwodo OFC
AD - Department of Biochemistry, University of Nigeria, Nsukka, Enugu State,
Nigeria.
AD - Department of Biochemistry, University of Mkar, Mkar, Nigeria.
LA - eng
PT - Journal Article
DEP - 20180928
PL - Netherlands
TA - J Tradit Complement Med
JT - Journal of traditional and complementary medicine
JID - 101605474
PMC - PMC6702234
OTO - NOTNLM
OT - Antioxidants
OT - Erythrocytes
OT - Haemolysis
OT - Membrane stabilization
OT - Mucuna pruriens
OT - Sickle cell
EDAT- 2019/08/28 06:00
MHDA- 2019/08/28 06:01
CRDT- 2019/08/28 06:00
PHST- 2017/02/21 00:00 [received]
PHST- 2017/05/19 00:00 [revised]
PHST- 2017/08/01 00:00 [accepted]
PHST- 2019/08/28 06:00 [entrez]
PHST- 2019/08/28 06:00 [pubmed]
PHST- 2019/08/28 06:01 [medline]
AID - 10.1016/j.jtcme.2017.08.002 [doi]
AID - S2225-4110(17)30094-9 [pii]
PST - epublish
SO - J Tradit Complement Med. 2018 Sep 28;9(4):278-284. doi:
10.1016/j.jtcme.2017.08.002. eCollection 2019 Oct.