H3-30 Service Manual V15.00

Revision History
Version Date Modification

15.00 15/04/2015 Initial Release
HEMIX 3-30_Technical Manual_V15.00 | 3

Table of Contents
1. GENERAL OVERVIEW ........................................................................ 15

1.1. FRONT VIEW ................................................................................................................... 15
1.2. REAR VIEW ...................................................................................................................... 16
1.3. IDENTIFYING INTERNAL ASSEMBLIES ............................................................................. 17
1.4. SYSTEM SPECIFICATIONS ................................................................................................ 20
1.4.1. Parameters ....................................................................................................................... 20
1.4.2. Volumes ............................................................................................................................ 21
1.4.3. Dilution ............................................................................................................................. 21
1.4.4. Input/Output .................................................................................................................... 21
1.4.5. Blank Test.......................................................................................................................... 21
1.4.6. Carry Over ......................................................................................................................... 21
1.4.7. Repeatability ..................................................................................................................... 22
1.4.8. Linearity and Precision ...................................................................................................... 22
2. INSTALLATION PROCEDURE ............................................................. 23

2.1. PRE-INSTALLATION ON SITE INSPECTION ....................................................................... 23
2.1.1. Space requirements .......................................................................................................... 23
2.1.2. Location requirements ..................................................................................................... 23
2.1.3. Power requirements ......................................................................................................... 23
2.2. PRE-INSTALLATION INSTRUMENT INSPECTION .............................................................. 23
2.3. UNPACKING / INSTALLING THE HEMIX 3-30................................................................... 23
2.4. PERFORMANCES VERIFICATION ..................................................................................... 27
2.4.1. Background ....................................................................................................................... 27
2.4.2. Stability : CV ...................................................................................................................... 27
2.4.3. Calibration ........................................................................................................................ 28
3. THEORY OF OPERATION ................................................................... 29

3.1. COUNTING BLOOD CELLS ............................................................................................... 29
3.1.1. Coulter principal ............................................................................................................... 29
3.1.2. Treating the signal ............................................................................................................ 30
3.2. HGB READING ................................................................................................................. 30
4. FLUIDICS SYSTEM .............................................................................. 31

4.1. FLUIDICS PHASES ............................................................................................................ 31
4.1.1. Stand by ............................................................................................................................ 31
4.1.2. Dilution ............................................................................................................................. 31
4.1.3. Counting ........................................................................................................................... 31
4.1.4. Cleaning ............................................................................................................................ 32

4.2. FLUIDICS SCHEMATIC ..................................................................................................... 32
4.3. FLUIDICS COMPONENTS ................................................................................................. 35
4.3.1. Counting Chamber Assy .................................................................................................... 35
4.3.2. Dilution block Assy ............................................................................................................ 37
4.3.3. Vacuum Block Assy ........................................................................................................... 40
4.3.4. Sample Probe Washer Assy .............................................................................................. 41
4.3.5. Solenoid Valves ................................................................................................................. 43
5. ELECTRONICS ................................................................................... 45
5.1. SWITCHING POWER SUPPLY ........................................................................................... 45
5.2. CENTRAL COMMAND UNIT, CCU BOARD ....................................................................... 45
5.2.1. Function ............................................................................................................................ 45
5.2.2. Functional Schematic ........................................................................................................ 46
5.2.3. Components Layout .......................................................................................................... 47
5.3. CENTRAL PROCESSING UNIT: CPU BOARD ..................................................................... 50
5.4. LIQUID SENSOR ADJ. /CONNECTION DISTRIBUTION BOARD ......................................... 51
5.5. LCD ADAPTOR BOARD .................................................................................................... 53
5.6. ON BOARD THERMAL PRINTER DRIVER BOARD ............................................................. 53
6. MECHANICS ..................................................................................... 55
6.1. SAMPLE PROBE ARM ...................................................................................................... 55
6.2. DILUTION MACHINE ASSEMBLY ..................................................................................... 56
6.3. VACUUM PUMP MACHINE ............................................................................................. 58
7. SERVICE SOFTWARE.......................................................................... 59
7.1. MECHANICS .................................................................................................................... 59
7.1.1. Electronic Valve ................................................................................................................ 60
7.1.2. Motor ................................................................................................................................ 60
7.1.3. Circuit ................................................................................................................................ 61
7.1.4. Interface............................................................................................................................ 62
7.2. SPECIAL ........................................................................................................................... 62
7.2.1. Factory mode .................................................................................................................... 62
7.2.2. Parameters screen ............................................................................................................ 62
7.3. NETWORK TRANSFERT PROTOCOL ................................................................................. 64
7.3.1. Introduction ...................................................................................................................... 64
7.3.2. Transfer protocol .............................................................................................................. 64
7.3.3. Wiring ............................................................................................................................... 64
7.3.4. Data block format ............................................................................................................. 64
7.3.5. Usage of WBC, RBC, PLT histogram data .......................................................................... 65
7.3.6. Example ............................................................................................................................ 65
7.3.7. Hardware to test communication ..................................................................................... 67
6 | HEMIX 3-30_Technical Manual_V15.00

8. ADJUSTMENTS AND CONTROLS ....................................................... 69
8.1. REAGENT ALARM TEST ................................................................................................... 69
8.2. COOLING FAN TEST......................................................................................................... 70
8.3. VALVES COMMUTATION TEST ........................................................................................ 70
8.4. MICROCONTROLER TEST ................................................................................................ 71
8.4.1. A/D Conversion ................................................................................................................. 71
8.4.2. INT..................................................................................................................................... 71
8.5. POWER SUPPLY: 5VDC .................................................................................................... 71
8.6. HGB BLANK VOLTAGE ..................................................................................................... 71
8.7. ACCURATE COUNTING VACUUM CHECK: MEASURING THE VACUUM .......................... 71
8.8. BURNING FUNCTION CHECK ........................................................................................... 73
8.9. DILUENT TEMPERATURE CHECK ..................................................................................... 73
8.10. SPEAKER CHECK .................................................................................................... 74
8.11. APERTURES VOLTAGES CHECK ............................................................................. 74
8.12. MOTORS CHECK ................................................................................................... 74
8.13. DILUENT DISPENSING CHECK ............................................................................... 74
8.14. REAGENT SENSORS SENSITIVITY CHECK ............................................................... 74
8.15. CHANNELS GAIN ADJUSTMENT ............................................................................ 74
8.16. APERTURE BASELINE VOLTAGE SETTING ............................................................. 75
8.16.1. Entering into factory mode ............................................................................................... 75
8.16.2. Performing Blank test ....................................................................................................... 75
8.16.3. Checking Channel Data Monitor ....................................................................................... 75
8.16.4. Setting Aperture baseline voltage .................................................................................... 75
8.17. SAMPLE PROBE MECHANICAL SYSTEM ................................................................ 76
8.17.1. Adjustment of the high position of the sample probe ..................................................... 76
9. MAINTENANCE ................................................................................. 79
9.1. USER MAINTENANCE ...................................................................................................... 79
9.1.1. Daily maintenance ............................................................................................................ 79
9.1.2. Weekly maintenance ........................................................................................................ 79
9.1.3. On demand maintenance ................................................................................................. 79
9.1.4. Maintenance log ............................................................................................................... 79
9.2. SFRI TECHNICAL MAINTENANCE .................................................................................... 80
9.3. PREVENTIVE MAINTENANCE PROCEDURE ..................................................................... 80
9.3.1. Spare parts, TOOLS and disposable .................................................................................. 80
9.3.2. Procedure ......................................................................................................................... 80
10. DISASSEMBLING/RE-ASSEMBLING ............................................. 93

10.1. REMOVING PANELS .............................................................................................. 93

10.2. THE CCU BOARD ................................................................................................... 94
10.3. THE FLUIDIC ASSEMBLY ........................................................................................ 95
10.3.1. Valves ................................................................................................................................ 96
10.3.2. Dilution Block .................................................................................................................... 97
10.3.3. Vacuum Pump ................................................................................................................... 98
10.3.4. Counting chamber ............................................................................................................ 99
10.3.5. Mechanical assembly ........................................................................................................ 99
10.3.6. Disassembling the sample needle mechanical system ................................................... 100
11. Troubleshooting ........................................................................ 103

11.1. ERROR / WARNING CODES LIST ......................................................................... 103
11.2. BACKGROUND TEST ABNORMAL ....................................................................... 103
11.3. NO DILUENT ....................................................................................................... 104
11.4. NO CLEANER ....................................................................................................... 104
11.5. NO LYSE .............................................................................................................. 104
11.6. HGB BUBBLE OR FAILURE ................................................................................... 104
11.7. HGB VALUE LOW ................................................................................................ 105
11.8. BUBBLE ............................................................................................................... 105
11.9. CLOG ................................................................................................................... 105
11.10. PLT HIGH............................................................................................................. 106
11.11. PLT LOW ............................................................................................................. 106
11.12. WBC HIGH .......................................................................................................... 106
11.13. INSTRUMENT INITIALIZATION FAILED ................................................................ 106
11.14. BOOT UP WHITE DISPLAY ................................................................................... 107
List of appendices
Appendix 1 Safety Symbols ................................................................................................................ 111
Appendix 2 Reagents and Consummables list.................................................................................... 113
Appendix 3 Installation Check List ...................................................................................................... 115
Appendix 4 Preventive Maintenance Check List ................................................................................ 117
Appendix 5 Spare Part List.................................................................................................................. 121
Appendix 6 Preventive Maintenance Kit Content .............................................................................. 123

Statement
SFRI Sarl owns all rights to this unpublished work and intends to maintain this work as confidential.
SFRI Sarl may also seek to maintain this work as an unpublished copyright. This publication is to be
used solely for the purposes of reference, operation, maintenance, or repair of SFRI Sarl equipment.
No part of this can be disseminated for other purposes.
In the event of inadvertent or deliberate publication, SFRI Sarl intends to enforce its rights to this work
under copyright laws as a published work. Those having access to this work may not copy, use, or
disclose the information in this work unless expressly authorized by SFRI Sarl to do so.
All information contained in this publication is believed to be correct. SFRI Sarl shall not be liable for
errors contained herein nor for incidental or consequential damages in connection with the furnishing,
performance, or use of this material. This publication may refer to information and protected by
copyrights or patents and does not convey any license under the patent rights of SFRI Sarl, nor the
rights of others. SFRI Sarl does not assume any liability arising out of any infringements of patents or
other rights of third parties.
Content of this manual is subject to changes without prior notice.
Responsibility on the Manufacturer Party

SFRI Sarl is responsible for safety, reliability and performance of this equipment only on the condition
that:
 All installation, expansion, change, modification and repair of this equipment are conducted
by SFRI Sarl qualified personnel;
 Applied electrical appliance is in compliance with relevant National Standards;
 The analyzer is operated under strict observance of this manual.
Warning
For continued safe use of this equipment, it is necessary that the listed instructions are followed.
However, instructions listed in this manual in no way supersede established medical practices
concerning patient care.
 This equipment is intended for use only by medical professionals in health care institutions.
 To avoid electrical shock, you should not open any cover by yourself. Service must be carried
out by qualified personnel only.
 It is dangerous to expose electrical contact or applicant coupler to normal saline, other liquid
or conductive adhesive. Electrical contact and coupler such as cable connector, power supply
must be kept clean and dry. Once being polluted by liquid, they must be thoroughly dried. If
to further remove the pollution, please contact your biomedical department or SFRI Sarl.
It is important for the hospital or organization that employs this equipment to carry out a reasonable
maintenance schedule. Neglect of this may result in machine breakdown or injury of human health.
Upon request, SFRI Sarl may provide, with compensation, necessary circuit diagrams, calibration
illustration list and other information to help qualified technician to maintain and repair some parts,
which SFRI Sarl may define as user serviceable.

Warranty
THIS WARRANTY IS EXCLUSIVE AND IS IN LIEU OF ALL OTHER WARRANTIES, EXPRESSED OR IMPLIED,
INCLUDING WARRANTIES OF MERCHANT ABILITY OR FITNESS FOR ANY PARTICULAR PURPOSE.
Workmanship and Materials

SFRI Sarl guarantees new equipment other than accessories to be free from defects in workmanship
and materials for a period of one year from date of shipment under normal use and service. SFRI Sarl's
obligation under this warranty is limited to repairing, at SFRI Sarl’s option, any part which upon SFRI
Sarl's examination proves defective.
Exemptions
SFRI Sarl's obligation or liability under this warranty does not include any transportation or other
charges or liability for direct, indirect or consequential damages or delay resulting from the improper
use or application of the product or the substitution upon it of parts or accessories not approved by
SFRI Sarl or repaired by anyone other than a SFRI Sarl authorized representative.
This warranty shall not extend to any instrument which has been subjected to misuse, negligence or
accident; any instrument from which SFRI Sarl's original serial number tag or product identification
markings have been altered or removed, or any product of any other manufacturer.
Safety, Reliability and Performance

SFRI Sarl is not responsible for the effects on safety, reliability and performance of the HEMIX 3-30 if:
 Assembly operations, extensions, re-adjusts, modifications or repairs are carried out by
persons other than those authorized by SFRI Sarl.
 Personal unauthorized by SFRI Sarl repairs or modifies the instrument.
Warning
The HEMIX 3-30 is an In Vitro Diagnostic instrument. It may indirectly bring risks to patients and
present a direct risk to operators and the environment during manipulation. To avoid these risks, be
sure to read the following instructions carefully.
This device uses AC 220V alternating courant. Do not open the rear cover before switching
off the power supply. If you need to open the rear cover of the device for maintenance
reasons, please contact our service personnel or professionals.
Patient samples are potentially contaminating agents. Manipulate with care using
appropriate protection equipment (glasses, gloves and laboratory coat) and be sure to
dispose of the waste in accordance with relevant regulations of your hospital.
Do not operate the device unless you have read this Operation Manual carefully

Company Contacts
Address : SFRI Sarl
Lieu-dit Berganton
33127 SAINT JEAN D’ILLAC
FRANCE
Phone : +33 (0) 556 688 050
Fax : +33 (0) 556 217 903
E-mail : support@sfri.com

1. GENERAL OVERVIEW
Class
According to anti-electric shock: Grade II, Pollution 2
According to defend deleterious liquid: General Equipment (Closed Equipment)
According to disinfection method: Recommend by manufactory
According to safety degree: Do not use the equipment under the environment of mixed with
flammable air or the air is mixed with O2 and NO
According to working state: Continuous running Equipment
Règlementation
DIV 98/79
1.1. Front View
Screen
Status
LED
Thermal
Printer Sample
Door Needle
Start
Key
Picture 1-1

1.2. Rear View
11
1
12
2
13
3 14
4 15
16
5
17
6
18
7
19
8
9 10 Picture 1-2
# Description
1 Parallel Printer Port
2 PS2 Mouse Port
3 PS2 Keyboard Port
4 Equipotential Connector
5 Power Switch
6 Lyse Inlet Tube Connector
7 Waste Full Sensor Connector
8 Waste Outlet
9 Cleaner Inlet Tube Connector
10 Diluent Inlet Tube Connector
11 Cooling Fan (Expulses air)
12 USB Port 1
13 USB Port 2
14 VGA Port
15 RS232 Port, COM 1
16 RS232 Port COM 2
17 Cooling Fan (Aspirates air, with air filter)
18 Power Socket with Fuses Housing
19 Switching Power Supply Cooling Fan
Table 1-1

1.3. Identifying internal assemblies
Picture 1-3 : Front view of the fluidic side
Picture 1-4 : 3D View of the complete fluidic side assembly

Picture 1-5 : Exploded view of the 3D fluidic side (locating sub-assemblies)
Mark Designation
1 Vacuum pump assembly
2 Dilution block assembly
3 Counting chamber
4 Three valves support assembly
5 Five valves support assembly
6 One valve support assembly
7 Mechanical assembly
8 Two valves support assembly
Table 1-2

Picture 1-6 : 3D view of the CCU board
Picture 1-7 : 3D view of the internal part of the front panel

Item # Description
1 CCU Board
2 CPU Board
3 Printer Driver Board
4 Start Latch
5 LCD Assy
Table 1-3
1.4. System Specifications

1.4.1. PARAMETERS
20 parameters and 3 histograms
1.4.1.1. MEASURED PARAMETERS
ITEMS UNITS
WBC 109 / L
RBC 1012 / L
MCV fL
HGB G/L
PLT 109 / L
MPV fL
Table 1-4
1.4.1.2. PARAMETERS GIVEN BY HISTOGRAM
ITEMS UNITS
LYM% %
MON% %
GRAN% %
RDW-SD fL
RDW-CV %
PDW %
P-LCR %
Table 1-5
1.4.1.3. CALCULATED PARAMETERS
ITEMS UNITS
LYM# 109 / L
MON# 109 / L
GRAN# 109 / L
HCT %
MCH pg
MCHC g/L
PCT %
Table 1-6

1.4.2. VOLUMES
Sample (µL) Diluent (mL) Cleaner (mL) Lyse (mL)

Venous 9,6 33,3 3,93 0,46
Capillary 9,6 33,3 3,93 0,46
Pre-Diluted 20 32,3 3,93 0,46
Table 1-7
1.4.3. DILUTION
Venous Capillary Pre-Diluted

WBC / HGB 1:300 1:300 1:355
RBC / PLT 1:44600 1:44600 1:44500
Table 1-8
1.4.4. INPUT/OUTPUT
Display Language
8” LCD screen English, French, Spanish
High resolution: 640x480 mm (Software not multilingual, dedicated
Color display Disc On Module requested)
Electrical input x 1 External VGA x 1

Parallel Printer Port x 1 RS-232 Ports x 2
USB Port x 2 PS/2 Ports x2
1.4.5. BLANK TEST
Parameter Result range Data range

WBC ≤0.2×109/L ≤200KB
RBC ≤0.02×1012/L ≤100KB
PLT ≤10×109/L ≤300KB
Table 1-9
1.4.6. CARRY OVER
Parameter Pollution range

WBC ≤0.5％
RBC ≤0.5%
HGB ≤0.5%
PLT ≤1.0%
Table 1-10

1.4.7. REPEATABILITY
Parameter Repeatability (CV %)

WBC ≤2.5%
RBC ≤2.0%
HGB ≤1.5%
MCV ≤0.5%
PLT ≤5.0%
Table 1-11
1.4.8. LINEARITY AND PRECISION
Parameter Linearity Range Parameter Linearity Range

WBC 0.0 -99.9x109/L GRAN# 0 -99.9x109/L
RBC 0.00 -9.99x1012/L HCT 0.0 -100.0%
HGB 00.0 -300g/L MCH 0.0 -999.9pg
PLT 0 -3000x109/L MCHC 0.0 -999.9g/L
MCV 0 -250fL RDW-SD 0.0 -99.9 fL
LYM% 0 -100% RDW-CV 0.0 -99.9%
MON% 0 -100% PDW 0.0 -30.0%
GRAN% 0 -100% MPV 0.0 -30.0fL
LYM# 0 -99.9x109/L PCT 0.0 -9.99%
MON# 0 -99.9x109/L P-LCR 0.0-99.9%
Table 1-12

2. INSTALLATION PROCEDURE
2.1. Pre-Installation on Site Inspection

2.1.1. SPACE REQUIREMENTS
HEMIX 3-30 measures 330(W) x 430(H) x 380(D) mm. There should be adequate space for the
instrument as well as the mouse, keyboard, external printer (optional), external barcode reader
(optional). Make sure that there is at least 15 cm of clearance around the top, sides and back of the
instrument. Ensure that there is adequate space for reagents either under the counter or at counter
level. Instrument weight is 17,5 kg. Make sure you will enough space to perform annual preventive
maintenance or on demand repair.
2.1.2. LOCATION REQUIREMENTS
The HEMIX 3-30 should be located in a controlled environment free from dust, mechanical vibrations,
loud noises and electrical / magnetic interferences. Avoid proximity to brush type motors, flickering
fluorescent lights and electrical contacts that regularly open and close. Avoid placing the instrument
in direct sunlight or in front of a source of heat or drafts. The instrument should be kept in a
temperature controlled environment between 18°C to 35°C (65°F to 95°F) with humidity between 10%
to 90% non-condensing.
2.1.3. POWER REQUIREMENTS
 HEMIX 3-30 requires a properly grounded outlet supplying 100 – 264 Va.c., 47 to 63 Hz. Power
consumption is 150 VA max.
 We recommend the use of a 300 VA UPS.
 Humidity should be less than 70%
 Atmospheric pressure has to be between 86.0 and 106 kPa.
2.2. Pre-Installation Instrument Inspection

A pre-installation inspection of the instrument upon arrival at the distributor’s facilities is strongly
recommended. Even though the HEMIX 3-30 is carefully packed, damage can occur during shipment.
Perform the installation procedures contained within this chapter to verify the operational integrity of
the HEMIX 3-30.
NOTE: theft or damage to the HEMIX 3-30 and related systems is not responsibility of SFRI France. It
is responsibility of the consignee to fill all claims with the freight carrier and to make sure that all
shipments are properly insured.
2.3. Unpacking / Installing the HEMIX 3-30

IMPORTANT: Read this section completely before powering on the unit. Any attempt to power on
without following this procedure will void warranty.

DO NOT SWITCH ON THE UNIT BEFORE COMPLETELY READING THIS SECTION
a) Examine the shipping containers for any damage. If there is any obvious damage,
photograph the damage.
b) Carefully remove the tape from the HEMIX 3-30 shipping container.
c) Open the container and examine the instrument for any signs of damage. If any
damage is present, photograph the damage and report it immediately to the carrier.
d) The instrument must not be installed in direct sunlight or near heating and air
conditioning vents.
e) Do not install the HEMIX 3-30 in carpeted areas. A static discharge may result and
damage sensitive components.
f) Remove the HEMIX 3-30 from the shipping container and place the instrument in its
final location. Save the shipping container and package inserts.
g) With the Allen wrench 2.5 remove the two screws of the right side panel (cf Picture 2-
1), and pull it out by sliding it backward.
Picture 2-1

Internal view:
1: Sample needle mechanical Assy

2: Dilution block Assy
3: Counting Chambers Assy
4: Vacuum pump Assy
Picture 2-2: Fluidic side
h) Take out the tie wrap blocking the sample arm like shown in the photograph bellow
Picture 2-4: 3D View of the tie Wrap
Picture 2-3: photography of guidance

system
i) Take out the tape covering the counting chamber, making sure you are not living any
piece of tape.
 Push gently the vertical guidance system to the left,
 Remove the tape.

Picture 2-5: photography of the vertical guidance
system
Picture 2-6: 3D View of the location of the tape
j) Remove the fluidic ports protectors in the back side of the unit
k) Connect the reagents supply tubes in the inlet fluidic ports (rear panel).
l) Place the reagents near the instrument.
DO NOT INCREASE THE LENGTH OF ANY OF THE REAGENT PICKUP TUBE

ASSEMBLIES.
m) Insert the reagent pickup tubes in their respective reagent. The reagent pickup tubes
have color coded luer caps that correspond to the luer connections on the rear panel
of the instrument. Remove the shipping plugs from the luer connections. Make sure
that the reagent pickup tubes are placed in the proper reagent.
(Green = SFRI Diluent 3.3, Blue = SFRI Cleaner 3.3, and Red = SFRI Lyse 3.3).
n) Connect the waste tube assembly to the back of the HEMIX3-30.
DO NOT INCREASE THE LENGTH OF THE WASTE TUBE ASSEMBLY.

o) Inflate the waste container and attach the waste tube assembly to the container.
Place the waste container on the floor. Waste may also be directed down a sink drain.
DISPOSE OF WASTE IN ACCORDANCE WITH GOVERNMENT REGULATIONS.

p) Save shipping containers in a cool, dry place.

2.4. Performances verification
2.4.1. BACKGROUND
We call background a measurement done without aspirating any blood, but air. In order to avoid
registering the results of background tests in the DATALOG, you may enter 999999 code in patient Id.
Press the Start Key without presenting any tube to the sample probe and let the instrument perform
a complete counting cycle. The results given by the unit should comply with the following table:
Item Range
WBC 0.0 – 0.2 x 109/L
RBC 0.00 – 0.02 x 106/µL
PLT 0 – 6 x 103/µL
HCT 0.0 – 0.5 %
Table 2-1
Should the Background results be out of tolerances, please refer to Trouble shooting section.
2.4.2. STABILITY : CV
Once the system is able to perform at least five backgrounds in range you can go through the stability
verification.
Run 15 replicates of a normal level SFRI blood control in factory mode (Refer to Software Chapter) and
make the instrument calculate for you the CV.
If the CV values are in range (see table 2-2 below), then see if they are in target, ie, if the results comply
with the control target. If they match, the system is ready to be used. If they are not you have to
perform a calibration.
PARAMETERS PRECISION CV%

WBC ≤2.5%
RBC ≤2.0%
HGB ≤2.0%
MCV ≤1.0%
PLT ≤5.0%
Table 2-2
SFRI Blood Control:

Name: BLOODTROL, Item Code: R021005, Expiration (close): 105 days after production, Expiration
(open): 2 weeks after opening the vial.
NEVER CALIBRATE A SYSTEM WHICH IS NOT STABLE

2.4.3. CALIBRATION
IMPORTANT NOTE:
HEMIX 3-30 System has two sets of calibration factors:
 One set is the factory one, and can be seen and adjusted in “Factory” mode of the software.
 The second set is the user one, and can be adjusted manually or automatically by the user, in
“Normal” software mode.
If your system is stable and the values are not in range, you may need to calibrate the unit. Use only
SFRI Calibration Blood, and follow the calibration procedure as described in Operator Manual version
HEMIX-3/30_Operator Manual_ENG_V14.00 or above.

3. THEORY OF OPERATION
3.1. Counting blood cells

3.1.1. COULTER PRINCIPAL
The Coulter principle states that particles pulled through an orifice, concurrent with an electric current,
produce a change in impedance that is proportional to the volume of the particle traversing the orifice.
This pulse in impedance originates from the displacement of electrolyte caused by the particle. The
Coulter principle was named for its inventor, Wallace H. Coulter when he was working for the
Department of Naval Research to standardize the size of solid particles in the paint used on US
battleships in order to improve its adherence to the hull. The principle has found commercial success
in the medical industry, in particular in hematology, where it can be applied to count and size the
various cells that make up whole blood.
Cells, being non-conducting particles, alter the effective cross-section of the conductive micro channel.
If these particles are non-conducting, the electrical resistance across the channel increases, causing
the electric current passing across the channel to briefly decrease. By monitoring such pulses in electric
current, the number of particles for a given volume of fluid can be counted. The size of the electric
current change is related to the size of the particle, enabling a particle size distribution to be measured,
which can be correlated to mobility, surface charge, and concentration of the particles.
Picture 3-1

OHM LAW
The Coulter principal basically deals with the well known Ohm low:
U=RxI
Where U is the voltage, R the resistance and I the current.
A constant current generator is applied to the electrodes of two chambers separated by an orifice,
filled with electrolyte (diluent). This current generates a base line voltage whose value changes while
blood cells are crossing the orifice.
As a result, the measured voltage is a direct function of the size of the cells crossing the apertures.
U = f(ps)
Where U is the voltage and ps the particule size.
3.1.2. TREATING THE SIGNAL
THREASHOLDS
Each cell crossing the apertures will create a signal, a voltage pulse, which amplitude will be compared
to triggers, allowing their classification on WBC, RBC and PLT. These thresholds are software values
that are inside the executable program. Neither the user nor the service people are able and allowed
to modify these values.
HISTOGRAMS
Each pulse will be analyzed and stored to a specific memory area, depending on its characteristics.
Once the counting time is over (after 12 seconds), the memorized data is processed and the histograms
are performed. It is then possible to get any histogram derived data.
3.2. HGB READING

Adding lyse in the blood, the red blood cell will be hemolyzed and hemoglobin released. Hemoglobin
and lyse form a new mixture, which can absorb the wavelength of 540nm. Absorbency is then
measured. Through the comparison of the absorbency between the pure diluent and the sample, the
concentration of the sample hemoglobin is calculated.
Picture 3-2

4. FLUIDICS SYSTEM
4.1. Fluidics phases

4.1.1. STAND BY
After initialization and between each sample counting, the unit is in standby mode.
All the reagents are primed and the chambers are filled with diluent.
Vacuum / Pressure chamber is at atmospheric pressure.
The constant current generator is off.
Burn voltage is off.
Dilution mechanism and vacuum /pressure mechanism are at home position while sample probe arm
is positioned ready to pipette a new sample blood.
All valves are off (i.e., not energized). Two ways valves are closed, and three ways valves are in NO
(Normally Opened) position.
HGB lamp is on.
Motors power is off.
4.1.2. DILUTION
The blood tube is presented to the sample probe, start switch is pushed, 9,6 µL of sample is aspirated.
A first dilution is done in the counting chamber. Dilution ratio is 1/300 in venous and capillary mode.
A little amount of volume is then aspirated to prepare the second dilution in the counting chamber:
Dilution ratio will be 1/44600. Lyse is dispensed to counting chamber: 460 µL. HGB is read by the
spectrophotometer. The RBC / PLT dilution will be done after WBC count and chamber cleaning. Air
bubble are sent by the underneath of the chamber in order to mix properly the dilution.
4.1.3. COUNTING
As soon as the first dilution is ready in the chamber, the vacuum/pressure pump generates an accurate
and stable counting vacuum. The cells are then hydro-focused in a fluidic current in order to be counted
during 12 seconds. At this step, only the WBC are counted.
The chamber is then emptied and cleaned in order to have the second dilution prepared. Using the
first dilution volume present in the needle together with additional diluent, the dilution is performed,
mixed, and the PLT and RBC are then counted during 12 seconds.
The software is controlling the consistency of cells circulation in order to detect any fluidics problem.
CAUTION: Vacuum value is not controlled by the system. It is important to run every morning a
quality control blood to make sure this function is performed properly.
In principal if the preventive maintenance is done according to SFRI recommendation, the vacuum
should be operating properly between at least two preventive maintenance visits. Should you suspect
any counting vacuum problem please use an external manometer to make sure this function is
complying with the specs.

4.1.4. CLEANING
Once the counting is finished, the chamber is emptied (to external waste), the counting line is cleaned
with cleaner primed by the vacuum pump. The chamber is cleaned internally with diluent and finally
filled again with diluent, in order to get back to standby mode. In the meantime the probe has been
cleaned internally as well with diluent.
4.2. FLUIDICS SCHEMATIC

See next page.

Picture 4-1

NO. Locations Tube spec.(Inner Dia*Outer Dia*Material) Length（mm） Connection
Sample 1.6*3.5mm*PVC+Temperature 60+Temperature Dilution

G1
needle Sensor+1.6*3.5mm*PVC+2.4*4.2mm*PVC Sensor+840+10 block-3
2.4*4.2mm*PVC+Straight 35+Straight Dilution

G2 V7-C
Through Tube +1.6*3.5mm*PVC Through Tube+130 block-1
Dilution
G3 V10-C 1.6*3.5mm*PVC 120
block-2
Dilution
G4 V8-NC 1.6*3.5mm*PVC 90
block-4
G5 V8-C 1.6*3.5mm*PVC 165 V10-NC
G6 V8-NO 1.6*3.5mm*PVC 210 V9-C
G7 V10-NO 2.4*4.2mm*PVC+DLS+2.4*4.2mm*PVC 160+DLS+200 DLS(green)
2.4*4.2mm*PVC+Straight 35+Straight
G8 V7-NC steel tube-2
Through Tube+1.6*3.5mm*PVC Through Tube+380
G9 V7-NO 2.4*4.2mm*PVC+LYS+2.4*4.2mm*PVC 130+LYS+200 LYS(red)
G10 V3-C 2.4*4.2mm*PVC 140 glass blub-1
G11 V3-NC 2.4*4.2mm*PVC 240 Vacuum-1
35+Straight Washer head-

G12 V2-NC 2.4*4.2mm PVC+1/16*1/8inch*PVC(E-3603)
Through Tube+280 1
G13 V2-C 2.4*4.2mm*PVC 160 Vacuum-2
35+Straight Washer head-

G14 V9-NO 2.4*4.2mm PVC+1/16*1/8inch*PVC(E-3603)
Through Tube+280 2
G15 V9-NC 2.4*4.2mm*PVC 40 Tee-tube
G16 V4-NC 2.4*4.2mm*PVC 35 Tee-tube
G17 V4-C 2.4*4.2mm*PVC+CLS+2.4*4.2mm*PVC 135+CLS+115 CLS（blue）
G18 Tee-tube 1.6*3.5mm*PVC 65 steel tube‐1
G19 V11-NC 2.4*4.2mm*PVC 130 glass blub‐2
G20 V11-C 2.4*4.2mm*PVC 95 Vacuum‐4

G21 V5-NC 2.4*4.2mm*PVC 185 Vacuum‐5

NO. Locations Tube spec.(Inner Dia*Outer Dia*Material) Length（mm） Connection
2.4*4.2mm*PVC+Straight 35+Straight
G22 V5-C steel tube‐2
Through Tube+1.6*3.5mm*PVC Through Tube+75
G23 V6-C 2.4*4.2mm*PVC 75 Vacuum‐6

waste（black
G24 V6-NO 2.4*4.2mm*PVC 200
）
G25 Chamber-1 1.6*3.5mm*PVC 85 steel tube‐1
G26 Chamber-2 1.6*3.5mm*PVC 65 steel tube-3
1.6*3.5mm*PVC+1.75*2.45mm*Teflon+1.6
G27 Chamber-3 40+80+40 Tee-tube
*3.5mm*PVC
G28 Tee-tube 1.6*3.5mm*PVC 35 steel tube-2
G29 Glass bulb 1.6*3.5mm*PVC 105 Tee-tube
G30 \ 2.4*4.2mm*PVC 50 steel tube-3
Table 4-1
4.3. Fluidics components

4.3.1. COUNTING CHAMBER ASSY
ITEM CODE : S020009-02
FUNCTION:
Two dilutions are performed in this chamber to allow blood cells counting.
A first dilution with 9,6 µL of whole blood pipetted by the sample needle directly from the patient tube
will be used to read HGB and count WBC. Before dispensing the Lyse, some volume of this first dilution
will be aspirated and maintained in the needle for a second dilution.
Once the counting is finished, the chamber is cleaned, and emptied, and the second dilution to count
RBC and PLT is done.
The aperture of this chamber has an 80 µm orifice and the aperture voltage range is between 13 Vdc
and 23 Vdc. The ruby separates two areas of the chamber; in each of them an electrode is located,
driving either the counting controlled constant current or the “high AC voltage”, in charge of the
burning function coming from the CCU board.
The electrodes of the chamber are connected to the CCU Board at J15
Picture 4-2 shows a detailes exploded view of the counting chamber.

Picture 4-2: Counting Chamber assembly, exploded view

4.3.2. DILUTION BLOCK ASSY
Picture 4-3: Dilution block (Global view) Picture 4-4: Dilution block (Exploded view)
ITEM CODE: S020024-01
FUNCTION:
The function of this assembly is to prime and aspirate / dispense reagents, sample blood (sample,
control, and calibrator). It is composed of a single Teflon block, in which 3 chambers and their
corresponding pistons are in charge to deliver accurate volumes of blood, diluent and lyse.
The three pistons are mechanically linked and thus are moving at the same time. The valves activation
program allows proper volumes delivery as required.
IMPORTANT: the sample piston has a specific design, making possible to control the other two pistons
without any consequence on the sample one. Two mechanical stops, located at specific position allow
this to be possible. Refer to picture 4-8 below (mark 2).
NEVER TOUCH THE TWO STOP NUTS, THEIR POSITION IS FACTORY ADJUSTED
AND NEVER HAVE TO BE CHANGED.
CAUTION: the part 4 shown in picture 4-8, is a precision Teflon guide. Please handle with care while
performing maintenance on the Dilution Block.

Picture 4-5: Dilution block assembly

SAMPLE PISTON ASSY DETAIL:
Picture 4-6: Sample piston assembly

4.3.3. VACUUM BLOCK ASSY
ITEM CODE : S020022
FUNCTION:
 Counting vacuum: provides accurate and stable vacuum for the counting phase
 Mixing: Provide positive pressure to send air bubbles to the chambers, mixing function
 Priming the cleaner during cleaning phase
 Waste the vacuum pump contain
Picture 4-7: Vacuum block assembly (Global view) Picture 4-8: Vacuum block assembly (Exploded view)
Picture 4-9: Vacuum block assembly

4.3.4. SAMPLE PROBE WASHER ASSY
ITEM CODE: S020026-01
FUNCTION:
Wash the external part of the sample probe after blood aspiration
Picture 4-10: Local exploded view of the sample probe washer assembly

Picture 4-11: Sample probe washer assembly

4.3.5. SOLENOID VALVES
ITEM CODE: S020007-02 (Two ways valve) ITEM CODE: S020008-02 (Three ways valve)
Picture 4-12 Picture 4-13
FUNCTION:
Valve Number Valve Type Valve Function

V2 Two ways Sample Probe Washer Aspiration
V3 Two ways Atmospheric pressure port
V4 Two ways Cleaner dispensing valve
V5 Two ways Counting valve
V6 Two ways Waste valve
V7 Three ways Lyse priming valve
V8 Three ways Diluent path selection (dilution or washer / aperture – V9)
V9 Three ways Diluent path selection (washer or aperture)
V10 Three ways Diluent priming valve
V11 Three ways Counting chamber draining valve
Table 4-2 j

5. ELECTRONICS
5.1. Switching Power Supply

ITEM CODE : S020056
FUNCTION :
 Provide +12Vdc and +5Vdc to CPU board (via CCU, and jumper JP4) and CCU board
 +5Vdc / 4A is intended to power the CPU board
 +12 Vdc is intended to power the CCU board
 Provide +5Vdc and +12Vdc to
NOTE:
 An internal trimmer allows the adjustment of these voltages, paying attention that the CPU
voltage mustn’t be lower than 4,5 Vdc
 Two fuses of 3,15A (delayed)
5.2. Central Command Unit, CCU board

5.2.1. FUNCTION
5.2.1.1. ACTUATORS
 Motors driver
o Motor A (1): Sample probe vertical movement
o Motor B (2): Sample probe horizontal movement
o Motor C (3): Dilution block movement
o Motor D (4): Vacuum / Pressure pump
 2 way valves
 3 way valves
 Constant current generator (coulter principal cells counting)
 High AC voltage for “burn” function
5.2.1.2. SENSORS
 Start switch (mechanical)

 Waste full (resistive)
 Reagents presence (optical): diluents, cleaner and lyse
 Diluent Temperature (resistive)
 HGB spectrometer
 Cells counts (resistive - WBC, RBC, PLT)

5.2.2. FUNCTIONAL SCHEMATIC
HEMIX 3-30
Switching CCU Board
Power To PC 104
Supply CPU
Probe Motor Home

Vertical A Sensor
Driver A
MAIN Micro Valves Solenoid
Controller FPGA Driver Valves
Probe Motor Home
Horizon B Sensor
tal Driver B
Dilution Motor Home

Block C Sensor
Driver C
From
Vacuum Motor Home
Block D Sensor
Aquisition Counting
RBC A/D
Driver D
Conv
Chamber
Micro
contr
olle
Diluent t°
T° sensor Sensor Filtering
Aquisition
HGB Sensor HGB

Assy, Counting Sensor PLT A/D
Chamber Controlle Micro Conv
r contr erter
olle Amplifying
Reagents
Reagents
Presence /
Optical
Waste
Sensors
Sensors
Internal
On board Printer WBC A/D
Thermal Driver Micro Conv
Printer contr erter
olle
Burn Constant
From Burn
Voltage Current
Transformer
Controller Generat
110 Vac
or
Mechanical Relay (Selection

« Burn » or Constant Current to
Electrodes)
To Counting
Chamber
Diagram Block
Picture 5-1

5.2.3. COMPONENTS LAYOUT
5.2.3.1. FUNCTIONAL VIEWS
Picture 5-2: Front view of the CCU board

Picture 5-3: Back view of the CCU board

1: HGB process module 8: Power supply IC
2: Analog amply circuit 9: Pump, valve drive
3: High-voltage burn 10: Step–motor drive
4: Liquid system module 11: Internal Thermal Printer
5: Temperature detect 12: Status LED, front face
6: A/D Data collect 13: Burn Transformer
7: Digital signal process 14: Counting chamber acquisition
Table 5-1 Functional View
5.2.3.2. CONNECTORS AND TEST POINTS
Picture 5-4

J1: Power Supply Connector (From Switching
TP1: Platelets signal
Power Supply
TP2: WBC signal J2: Factory purposes
J3: Motors A to D flat cable connector (To
TP3: HGB signal
distribution Board)
TP4: 5 Vdc J4: PC104 CPU connector
TP5: 2.5 Vdc J5: PC104 CPU connector
J6: Solenoid Valves flat cable connector (To
TP6: 5 Vdc
distribution Board)
J7: Reagent sensors, Start, Waste Full… To
TP7: 5 Vdc
distribution Board)
TP8: 2.5 Vdc J8: Not used
TP9: 2.5 Vdc J9: Diluent temperature sensor
TP10: Ground J10: HGB sensor
Table 5-2 Main test points and connectors
5.3. Central Processing Unit: CPU board

FUNCTION :
This is the HID of the instrument. This industrial computer provides all basic input and output system
for the user, and communication with machine level software, ie, communication with the CCU
firmware through a PC104 bus.
A compact flash memory, or a Disc On Module, connected to the CPU, contains all BIOS, OS and
application software, allowing the unit to operate. The picture below shows all the connectors detail.

Picture 5-5
5.4. Liquid Sensor Adj. /Connection Distribution

Board
FUNCTION :
This board interfaces the connections between the CCU board and the actuators and sensors.

It also has a dedicated electronics to adjust the optical reagent detection sensitivity, as shown
below.
⑥ ⑦ ⑧
⑨ ⑩
① ② ③
④ ⑤
Picture 5-6
# Area contain Description

1 J2 Lyse optical detector
2 J4 Cleaner optical detector
3 J6 Diluent optical detector
4 TP1 Test point for lyse Optical Sensor adjustment
TP2 Test point for cleaner Optical Sensor adjustment
TP3 Test point for diluent Optical Sensor adjustment
VR1 Lyse OS sensitivity adjustment trimer
VR2 Cleaner OS sensitivity adjustment trimer
VR3 Diluent OS sensitivity adjustment trimer
5 J5 Waste sensor connector
J7 Start switch connector
Electronics To adjust the sensitivity of the Optical Sensors
6 J1 Transfers all the above data to CCU Board
TP4 Ground for TP1 to TP3
7 J10 Valves command entry (from CCU Board)
8 J25 Motors and home sensors signals from and to CCU
9 V2 to V11 Valve connectors
10 MA to MD Motors A, B, C and D
OSA to OSD Home sensors for motors A to D
Table 5-4

5.5. LCD Adaptor Board
ITEM CODE: S020067
FUNCTION:
The main function of LCD Adaptor Board is to connect the screen to the CPU with cable. It interfaces
TFT through FPC soft cable.
Picture 5-7
5.6. On Board Thermal Printer Driver Board

ITEM CODE: S020028 (Complete assembly, included printer head)
Picture 5-8

6. MECHANICS
6.1. Sample probe arm

Picture 6-1

N° Component N°Article Description
1 NA Linear guide
2 NA Washer upper part
3 NA Washer lower part
4 S020025-01 Sample Needle
5 NA Circlips
6 NA Toothed pulley 1
7 S020055 Belt
9 NA M3 Screw
10 S020021 Stepper Motor
11 S020021 Stepper Motor
12 S020005-02 Optical coupler assy, home sensor
13 NA Linear guide
14 NA Rod
15 NA Circlips
17 S020055 Belt
19 NA M3 Screw
Table 6-1
FUNCTION:
This mechanical assembly allows the system to position the sample probe at operator disposal, to
aspirate the sample, calibrator or control blood and then to the counting chambers, in order to
perform the dilutions.
While performing maintenance, the mechanical system will cover the same function but SFRI Clair 3.3
or SFRI CleanEZ 3.3 will replace the blood.
Two movements are carried out to perform these positioning: vertical and horizontal.
Thus, two stepper motors are involved, Motor A (vertical movement), and Motor B (horizontal
movement). Each movement has a home position, controlled by an optical sensor.
6.2. Dilution Machine Assembly

ITEM CODE: S020023
FUNCTION:
The rotational movement of the stepper motor is transformed in a linear vertical movement by a gear
and a rod. An optical sensor stops the mechanics in its home position.
The rod drives vertically a bracket that maintains the three pistons of the dilution block together. As a
results all the pistons but the sample one are moving together. The sample piston has a particular
design that allows the piston to remain in place while the others are moving.
The bearings bracket and the pulley are helping the vertical movement to be done smoothly.

Picture 6-4
Item Number Item Code Description

1 NA Chassis
2 NA Bearing
3 S020021 Stepper motor
4 S020054 Gear
5 NA Spacer
6 NA Metal bracket
7 NA Bearing
8 NA PTFE Pulley
9 NA Rod holder
10 NA
11 S020066 Rod for dilution machine
12 NA Pistons bracket
13 NA Home sensor flag
14 S020005- Home sensor
15 NA Screws
Table 6-2

6.3. Vacuum Pump Machine
ITEM CODE:S020064
FUNCTION:
The rotational movement of the stepper motor is transformed in a linear vertical movement by a gear
and a rod. An optical sensor stops the mechanics in its home position.
Picture 6-5
Item number Item Code Description

1 NA Motor bracket
2 S020053 Metal bracket with bearings
3 S020021 Stepper motor
4 NA Screws
5 NA Bearing
6 NA Bearing
7 S020054 Gear
8 NA PTFE Pulley
9 NA Spacers
Table 6-3

7. SERVICE SOFTWARE
7.1. Mechanics
This Service Program allows the service engineer to troubleshoot, by checking actuators and sensors.
This program sends single commands to the CCU.
From the main screen, select Service, Mechanic.
Picture 7-1

Enter password 6800 when prompted. You will reach the following screen.
Picture 7-2
We do have four main parts in this screen:
7.1.1. ELECTRONIC VALVE
Here we can activate each valve for a couple of seconds by clicking on its “T” command.
Doing so, you have to hear the commutation sound, which is louder for the two-way valves.
NOTE: Valves 1, is not used. The “T” command is grey, and a click on it has no effect.
7.1.2. MOTOR
A click on “T” command of each motor will generate a movement to get the mechanical part to home
position. If the mechanism is already at home position, the firmware will move the flag out and in
again. If the mechanism is out of home position, the firmware will move the flag toward home position.
If the system does not detect any error on the movement the software will send an “Ok” status,
otherwise “Failed” will be prompted.
 Motor A: Vertical Movement of the Sample Probe Arm
 Motor B: Horizontal Movement of the Sample Probe Arm
 Motor C: Dilution Block Movement
 Motor D: Vacuum Pump Movement

7.1.3. CIRCUIT
This part of the Mechanic software is dedicated to test the CCU board
7.1.3.1. ANALOGICAL / DIGITAL CONVERTOR
WBC AD / RBC AD / PLT AD: test of the Analogical to Digital converters for each channel. There are
three converters, one by cell type. The system sends a value to convert and the software tests the
feedback. The system returns “OK” if the returned value is the good one and “Failed” if not.
7.1.3.2. INTERRUPT REQUEST / MICRO CONTROLLER
This function allows to test the interruptions control between main micro-controller of the CCU and
the dedicated 3 micro-controllers in charge of the cell pulses acquisition (WBC, RBC, PLT pulses).
7.1.3.3. 5V POWER
This is an integrated voltmeter returning the actual +5 Vdc value.
7.1.3.4. HGB
This function returns the actual HGB sensor reading. Please refer to Adjustments and Controls chapter
for more details.
7.1.3.5. VACUUM
This function creates a specific vacuum value (counting vacuum). Activating this command will make
the vacuum piston move up to predefined specific position.
7.1.3.6. DC POWER
This is the “Enable” signal sent by the software to the CCU board.
7.1.3.7. CC POWER
This function allows you to test the Constant Current generator. A click on its “T” command will activate
relay K3, as a result the Constant Current will be sent to the electrodes of the counting chamber in
order to generate the base line voltage.
7.1.3.8. BURN
This function sends 110 Vac directly to the electrodes of the counting chamber in order to clean the
aperture and prevent clogs that could appear due to orifice diameter reduction while proteins get
agglutinated. Relay K4 is activated to send this “high” AC voltage to the chamber.
7.1.3.9. R. TEMP
This function returns the actual diluents’ temperature, measured by the sensor integrated in the
sample line.
7.1.3.10. SPEAKER
Internal speaker test. You should hear a little “pock” sound.

7.1.3.11. HOLE
This function is returning the aperture voltage value while clicking on “T1”.
The system will generate a vacuum, open the counting valve V5, and send the constant current to the
electrodes in order to generate the base line voltage. The current aperture voltage will be displayed
after a couple of seconds.
The values have to be as follow: Hole voltage between 11 and 25 Vdc
Then the system goes back to stand by, ie, stops the constant current and the diluent circulation. The
measured value remains in the screen.
An action on the T2 command will refresh the actual baseline voltage value that has to drop below 3
Vdc. Typical value is around 1,5 Vdc.
7.1.4. INTERFACE
You can test the three peripheral dedicate to receive results.

 On board thermal printer (Recorder)
 External Printer
 RS232 Serial Port
7.2. Special
7.2.1. FACTORY MODE
From main screen :

 Service -> Special -> Enter password « 6666 ».
 Press F12
 Check that the HEMIX3-30 is actually in Factory Mode (you should see SFRI France in the
bottom left corner of the screen, and “Factory” appears in the main title screen (Top center).
The two principal functions you access in factory mode are:

 The factory calibration factors
 CV calculation functionality
NOTE: In factory mode, the apertures voltages are displayed in the left bottom area of the screen, in
red. This functionality allows you to check if the aperture voltages are stable and in range.
Pressing F12 will switch from normal to factory mode unless you enter again 6666 password as
explained above, or instrument is powered off.
IMPORTANT NOTE: never leave the unit with factory mode accessible for the end user.
All the standard functions of the unit are the same than in user mode.
7.2.2. PARAMETERS SCREEN
You should print and save these parameters before sending the unit at customer site
Access to this screen: Service -> Special -> Enter password 6868

The “Parameters Adjust” screen is shown here below:
Picture 7-3
The parameters adjust screen is divided in two parts.
7.2.2.1. PARA
Do not attempt to adjust these parameters, they are factory set and do not need any adjustment in
the field, unless SFRI authorized specialist indicates you to perform changes there.
7.2.2.2. FLUIDICS
WBC & RBC Time (s): Software fixed at 12 seconds. This value must not be changed.
WBC & RBC Hole: These are the reference values that the system uses in order to return a clog
situation. Refer to adjustment chapter for procedure.
WBC & RBC threshold: Factory values that do not have to be changed.
Temp. Low: sets the minimum room temperature allowed to the system to work.
Temp High: sets the maximum room temperature allowed to the system to work.
Motor A, B, C and D: motor steps for mechanics positioning. Factory adjusted, they do not have to be
modified.

7.3. Network Transfert Protocol
7.3.1. INTRODUCTION
SFRI HEMIX3-30 hematology analyzer uses RS-232 port as a network transmission. There are
three data lines on transmission: TxD, RxD, GND. The data transfer to the receiving device by
a one-way transmission, not response of data request for any external device. This protocol
adopts characteristic identification method and data block on transmission. All the decimal
points could not be transferred in practice.
7.3.2. TRANSFER PROTOCOL
Baud Rate 9600

Data Bits 8
Parity None
Stop Bits 1
Table 7-1
7.3.3. WIRING
Picture 7-4
7.3.4. DATA BLOCK FORMAT
Start-identifier of data block: @ MCH: ###.#

Identifier of data block: a Alarm: # (#: normal, h: high, l: low)
Sample No.: ########### MCHC: ####
Year: #### Alarm: # (#: normal, h: high, l: low)
Month: ## RDW-SD: ##.#
Date: ## Alarm: # (#: normal, h: high, l: low)
Hour: ## RDW-CV: ##.#
Minute: ## Alarm: # (#: normal, h: high, l: low)
Second: ## PLT: ###

WBC: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) MPV: ##.#
LYM%: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) PDW: ##.#
MID%: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) PCT: #.##
GRAN%: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) P-LCR: ##.#
LYM#: ##.# Alarm: # (#: normal, h: high, l: low)
Alarm: # (#: normal, h: high, l: low) Reserved: ########
MID#: ##.# Low temperature: #(#: normal, t: temperature)
Alarm: # (#: normal, h: high, l: low) WBC bubble: #(#: normal, b: bubble)
GRAN: ##.# WBC clog: #(#: normal, c: clog)
Alarm: # (#: normal, h: high, l: low) RBC bubble: #(#: normal, b: bubble)
RBC: ##.# RBC clog: #(#: normal, c: clog)
Alarm: # (#: normal, h: high, l: low) Reserved: ########
HGB: ### RM: #(#: normal, r:alarm)
Alarm: # (#: normal, h: high, l: low) R1: #(#: normal, r:alarm)
HCT: ##.# R2: #(#: normal, r:alarm)
Alarm: # (#: normal, h: high, l: low) R3: #(#: normal, r:alarm)
MCV: ###.# R4: #(#: normal, r:alarm)
Alarm: # (#: normal, h: high, l: low) PM: #(#: normal, r:alarm)
Reserved: ########
Lower limit of WBC first line: ###
Upper limit of WBC first line: ###
Lower limit of WBC final line: ###
Upper limit of WBC final line: ###
Lower limit of RBC: ###
Upper limit of RBC: ###
Lower limit of PLT: ###
Upper limit of PLT: ###
Reserved: ########
WBC histogram: ### (total number: 256)
RBC histogram: ### (total number: 256)
PLT histogram: ### (total number: 256)
End-identifier of data block: &
7.3.5. USAGE OF WBC, RBC, PLT HISTOGRAM DATA
The total number of the received data between (0,255) is 256. If there are 256 points on the
X-axis of the histogram, set the proportion of Y-coordinates as “a”. Divide the 256 received
data respectively by “a”, the results as the values of Y-coordinates. Draw the corresponding X-
coordinates in the received order.
7.3.6. EXAMPLE
The following is a complete data flow example.
Start-identifier of data block: @ Alarm: #

Identifier of data block: a HCT: 361
Sample No.: 050603008 Alarm: #

Year: 2005 MCV: 0806
Month: 06 Alarm: #
Date: 03 MCH: 0226
Hour: 21 Alarm: #
Minute: 43 MCHC: 0263
Second: 08 Alarm: #
WBC: 069 RDW-SD: 465
Alarm: # Alarm: #
LYM%: 269 RDW-CV: 150
Alarm: # Alarm: #
MID%: 121 PLT: 117
Alarm: # Alarm: #
GRAN%: 610 MPV: 097
Alarm: # Alarm: #
LYM#: 019 PDW: 113
Alarm: # Alarm: #
MID#: 008 PCT: 011
Alarm: # Alarm: #
GRAN: 042 P-LCR: 141
Alarm: # Alarm: #
RBC: 420 Reserved: ########
Alarm: # Low temperature: #
HGB: 095 WBC bubble: #
WBC clog: #
RBC bubble: #
RBC clog: #
Reserved: ########
RM: #
R1: #
R2: #
R3: #
R4: #
PM: #
Reserved: ########
Lower limit of WBC first line: 020
Upper limit of WBC first line: 057
Lower limit of WBC final line: 082
Upper limit of WBC final line: 255
Lower limit of RBC: 010
Upper limit of RBC: 164
Lower limit of PLT: 010
Upper limit of PLT: 214
Reserved: ########
WBC histogram: ###
RBC histogram: ###
PLT histogram: ###
End-identifier of data block: &
Here is the flow itself :
@a05060300820050603214308069#269#121#610#019#008#042#420#095#361#0860#0226#0263#
465#150#117#097#113#011#141####################################0200570822550101640

10214########00000000000000000000000000000000000000000000000000000000100200200300
500700901201201401902402903704205005806607007507607808007707607006906606005404704
303803703002602502202001901701501801701802002102302402902903203403303403403603403
503403003103002802602502302202202102002001901801801901901902002102102102202202302
302502802903303103403603804004004204504704705005205305605705906006206206005805605
505405405004804604404103903803603503403503403503302802802502402102001801801901601
601601501501501501401401201201201201101001000900900700600600700700600700700900800
700700700800700700600700700700700500500500500500500400400500500500500500500500500
400400400400400400400500500500500500500400400400400500500500500500600600600500500
500500500500500500600700600700801001001001001001001000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000100100100200200300
300400500500600700800901101301401801902302503003103503904304605005305206006306506
907007307308007507507407207106906706206105805605305004704504404103703603303202902
702402302101901801601501401401201101000900900800700600600500500500400400400400400
300300200200200200200200200200200200200200200200100100100100100100100100000100000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000300300700701401402202203003003903
904804805605606306306806807007006906906506506206205905905705705305305005004704704
604604604604604604604604504504604604604604504504304304104103903903703703603603203
203003002702702502502502502402402202202002001901901701701701701601601401401301301
201201201201201201101101001000900900900900900900800800700700700700600600600600500
500500500500500500500600600500500400400300300300300300300300300300300300300300300
400400500500600600600600600600600600600600500500400400300300300300200200200200200
200200200300300300300300300300300300300300300300300300300300300300300400400500500
600600600600600600600600600600600600600600600600600600600600700700700700800800900
900900900900900900900900900900900900900900900900904804804804805605600000000000000
000000000000000000000000000000000000000000800800000004304300000002102100000000300
300000000600600000021321300700700000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
024024021621603203208908900000001501500000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
000000000000000000000000000000000000000000000000000000000000000000000000000000000
00000000000000000&
7.3.7. HARDWARE TO TEST COMMUNICATION
Use Hyper Terminal or HTerm and a female / female DB9 crossed RS232 cable, using only RxD, TxD
and GND.
The data reception with HTerm communication software is illustrated bellow.

Picture 7-5
The related data of the transfer is shown in the following result printout
Picture 7-6

8. ADJUSTMENTS AND CONTROLS
After each Preventive Maintenance, or as required during troubleshooting, error or warning messages,
some controls and / or adjustments may be performed. You will read bellow the detailed explanation
/ procedure for each adjustment and control.
8.1. Reagent Alarm Test

Select Settings — Setting — Config, as show in picture 8-1, in the pop-up menu (Picture 8-2), set Alert
in the Sound to On, select Ok to return.
Picture 8-1
In this screen you can allow automatic printing, set the Sleep time and set the clean period.
You can also decide to print or not the limits.

Picture 8-2
At this time, it should issue a crisp Di Di sound, Waste Full alarm will be displayed in the right bottom
area of the screen.
When the system runs out of reagent, Diluent Empty, Lyse Empty and Cleaner Empty alarm
information will be displayed in the error message area of the screen.
Unplug the waste liquid short circuit connector on the back board of the HEMIX3-30, power off and
on. The instrument should be able to start, without any warning message.
8.2. Cooling Fan Test

In the back panel of the unit two fans are in charge to keep proper internal working temperature.
One fan is aspirating air from outside (this fan has a dust filter and it is located at the bottom), the
other is extracting warm air from the inside of the unit, it is located on the top.
Make sure those fans are operating smoothly, without noise, and that the air filter is clean.
Replace Fan and clean or replace air filter as required.
8.3. Valves Commutation Test

Select Service, Mechanic, enter password 6800.
The Electronic Valve area allows you to commutate each valve separately in order to ensure it is
operating properly.
Clicking on T button makes the corresponding valve commutate, remaining open for a couple of
seconds. You must hear the mechanical sound of commutation, taking in account that two way valves
have a louder sound than the 3 way valves.

8.4. MICROCONTROLER TEST
8.4.1. A/D CONVERSION
The analogical to digital converter is automatically checked by activating the T command of each A/D
converter (WBC, RBC and PLT). The software sends a command with data to convert and collects the
expected result. The status screen will display OK or FAILED.
8.4.2. INT
The Interruption Controller is responsible for the cells signals acquisition. To check the interruptions
request click T command of each channel, each microcontroller. The status screen will return OK or
FAILED.
8.5. Power Supply: 5Vdc

Click on T command of the 5V Power. The status screen have to display 5 Vdc +/- 0,5. An adjustment
can eventually be done on the switching power supply itself. A trimmer adjusts both together the +12
Vdc and the +5 Vdc.
8.6. HGB Blank Voltage

Click T command of the HGB, actual value is displayed. The target is 4,31 Vdc +/- 0,2 Vdc. If the value
has to be adjusted, using a special trimmer screwdriver, adjust VR5 trimmer on CCU board (figure 8-
3). To refresh the displayed current value while adjusting the trimmer, you have to click on the T button
simultaneously.
NOTE: turning VR5 clockwise decreases HGB value / counterclockwise increases HGB value.
IMPORTANT: you have to make sure before adjusting that the chamber is clean and filled with diluent,
properly primed.
Refer to section 5.2.4.1 for trimmer location.
IMPORTANT: two error messages are related to the HGB, depending on the blank voltage value read
during the test.
HGB BUBBLES: if the blank voltage is lower than 3 Vdc

HGB LAMP FAILURE: blank voltage higher than 4,8 Vdc
8.7. Accurate Counting Vacuum Check: Measuring

the vacuum
i. Open the right panel of the unit by unscrewing the two Allen screws (2.5 mm)
You can access to the fluidics panel
ii. Disconnect the tubing as shown in photograph 8-3 :

Picture 8-3
iii. In the same fitting of the vacuum pump, connect the vacuum meter tubing, as shown I
photograph 8-4
Picture 8-4
Note: make sure you gauge is at scale (0 at atmospheric pressure).
iv. Enter into the “Mechanic” function of the software
v. Click on “Vacuum” button, piston pump will go up…

Picture 8-5
You have to read negative pressure around 250 mbar +/- 10 mbar.
Decrease of this vacuum have to be less than 2 mbar per 2 minutes.
vi. Click again on T button of Vacuum function. The piston will go down to home position.
vii. Disconnect the manometer tubing, and put in place the original tubing.
8.8. Burning Function Check

When click T button of Burning function, you can hear a sound directly from the chamber (be careful,
the chamber should be fill of diluent).
Using a voltmeter with chambers full of diluent:
 Open the right panel of the unit by unscrewing the two Allen screws (2.5 mm), you can access
to the fluidics panel
 Set voltmeter to Vac
 Introduce the red probe of the voltmeter inside the WBC/RBC chamber and apply the black
probe to ground,
 Click on T button of Burning function
 You should read a value around 90 Vac (the relay remains switched a very short time, so the
reading of the target value is furtive).
8.9. Diluent Temperature Check

Click on T command of R. Temp function. The system returns on status field the diluent temperature
in the piece of tube where the sensor is located.
This value indicates the diluent chamber temperature +/- 5°C.
Use an external calibrated thermometer in order to check the value given by the HEMIX 3-30.
NOTE: HEMIX 3-30 has been designed to work between 18°C and 35°C.
Make sure the room temperature where the unit is located complies with this requirement.

8.10. Speaker Check
Click on T command of Speaker allows the system to issue a sound from the buzzer of the CPU board.
8.11. Apertures Voltages Check

Clicking T1 command will initialize the fluidics to check the apertures voltages (Vacuum will be
generated and counting valve opened). CCU board will send to the electrodes the constant current,
and the related voltage will be read by the system.
The voltages values have to be in the following ranges:
hole voltage: 11 to 23 Vdc
Clicking T2 command will switch off the constant current to the electrodes. At that moment the
residual voltage displayed have to be less than 3 Vdc for both chambers.
8.12. Motors Check

This function will bring the motors A, B, C or D to their home position. Should any of them be at home
position already, it will be driven out and in again.
The intent of this function is to check the motion, smooth, without noise, and the home sensor
positioning.
8.13. Diluent Dispensing Check

Click “Settings”, “Sample modes”, “Prediluted” to enter into the prediluted measuring mode, and then
click “Service”---“Diluent”, put a 10ml graduated cylinder under the sampling needle, press the start
button, continuous add diluent 5 times, the total amount should be 8.0±0.4ml, and click “exit”.
Set sample mode back to Venous.
8.14. Reagent Sensors Sensitivity Check

Set voltmeter to DC, and connect black probe to ground and the red probe to TP1, TP2, and TP3 of
cable board, monitor the relative voltage, there are the output voltage of lyse, cleaner and diluents.
When reagent is present in the liquid sensor, the voltage should be 3 - 5Vdc, when no reagent is
present in the sensor the voltage should equal to or less than 1,5Vdc, otherwise adjust the VR1, VR2
or VR3.
8.15. Channels Gain Adjustment

This procedure is provided during technical training from SFRI. Gains are factory adjusted and do not
need to be touched in the field.

8.16. Aperture Baseline Voltage Setting
8.16.1. ENTERING INTO FACTORY MODE
Click “Service”—“Special”, in the pop-up message box input password: “6666”, click OK, press F12 on
the keyboard, to make the host enter into “Factory” mode “the system interface above should display
“factory”
8.16.2. PERFORMING BLANK TEST
Under the venous mode, run 5 backgrounds, blank test (press start switch without presenting any
sample). Each background result should be in specs. If the blank tests are out of tolerances, cleaning
or strong cleaning procedure may be required, as well as burn function.
8.16.3. CHECKING CHANNEL DATA MONITOR
After each blank test, press F5, F6 and F7 in order to monitor the pulse graph and data amount for
WBC channel, RBC channel and PLT channel, the pulse graph of each channel should have no disturb
noise “the good pulse picture should smooth ”, the data amount should meet the requirement below.
WBC ≤200KB (Displayed 200 000)
RBC ≤100KB
PLT ≤300KB
8.16.4. SETTING APERTURE BASELINE VOLTAGE
Record the W.H.V.S. and R.H.V.S. after each test, calculate the average of 5 times W.H.V.S. and
R.H.V.S., and input into the Parameters (click Service----Special, in the pop-up message box input WBC
Hole and RBC Hole under password 6868)
Picture 8-6

8.17. Sample Probe Mechanical System
8.17.1. ADJUSTMENT OF THE HIGH POSITION OF THE SAMPLE PROBE
The function of the retaining ring shown in the picture bellow is to ensure the good positioning of the
sample probe if ever you have to take off the probe from the system for maintenance.
Picture 8-7
There are two possibilities:
1. YOU WANT TO TAKE OUT THE RETAINING RING FOR THE MAINTENANCE OF THE SAMPLE
PROBE :
For the reassembly, you need to find the exact position of the sample probe, using the adjustment
tool below, and following the instructions.
Picture 8-8: Sample probe adjustment tool
a) To take out the sample probe, you need to loosen the retaining ring,

b) Position the sample probe and the adjustment tool as scrupulously as shown on the pictures
below.
Picture 8-11

Picture 8-12
The tool has to be inserted by the underneath of the washer, and lift to the top. The probe
has to be pulled down till it touches the adjustment tool.
c) Now you can fix this position by tightening the retaining probe ring with its little screw
2. YOU DO NOT NEED TO TAKE OUT THE RETAINING RING :

You just have to replace the sample needle correctly as shown on the pictures below.
The retaining ring gives the proper positioning.

9. MAINTENANCE
9.1. User maintenance

9.1.1. DAILY MAINTENANCE
 Ask the user to perform a background before starting the working day. Check that the result is
in range.
 Run a QC SFRI bloodtrol and check that the result is in target.
 At the end of the day, perform a Shutdown using the SFRI CleanEZ 3.3 when the instrument
prompts you.
 Clean the unit panels with neutral detergent. Clean the screen, keyboard and mouse as
requested with adequate cleaner.
9.1.2. WEEKLY MAINTENANCE
At the end of a working week, it is recommended to run the maintenance procedure, launching it from
Service, Maintenance, and using SFRI Clair 3.3
9.1.3. ON DEMAND MAINTENANCE
For any brake down, malfunction, that the user is not able to handle, the end user representative will
have to contact the local SFRI representative technical service, in order to proceed immediately to the
relevant repair actions.
9.1.4. MAINTENANCE LOG
Frequency Operation Reagent / Material Potential trouble

Incorrect backgrounds
Daily Run “shut down” SFRI CleanEZ 3.3 Inaccurate results
Clogs in fluidic
Neutral detergent
Daily Clean up front panel Contamination
Cloth
Abnormal results
Weekly Run “Enhanced Flush” SFRI Clair 3.3 Incorrect backgrounds
Clogs in fluidic
Abnormal results
Weekly Run “Maintain program” SFRI CleanEZ 3.3 Incorrect backgrounds
Clogs in fluidic
Electronic troubles
Overheat
Monthly Clean up the air filter Brush
Abnormal counts
Clogs
Electronic troubles
Neutral detergent Abnormal counts
Monthly Clean up the analyzer
Cloth Clogs
Biological contamination
Table 9-1

9.2. SFRI Technical Maintenance
SFRI strongly recommends performing a complete preventive maintenance at least once a year, or
every six months if the number of samples per day is equal or higher than 30.
This maintenance has to be performed by a trained certified service engineer working for an official
SFRI distributor. Any authorized service engineer is able to prove his habilitation by presenting an
official SFRI authorization document.
The main operations that have to be performed during preventive maintenance (normally performed
in a three hours), are summarized in the PM (Preventive Maintenance) checklist in appendix 4, and the
main steps explained in the procedure bellow.
9.3. Preventive Maintenance Procedure

9.3.1. SPARE PARTS, TOOLS AND DISPOSABLE
 Ultrasonic Cleaning Device

 Distilled water
 Hemaclair / Diluclair
 Pressure meter, mulimeter
ITEM DESIGNATION: Preventive Maintenance Kit HEMIX 3-30 - 1 year

ITEM CODE: PMK0212
9.3.2. PROCEDURE
9.3.2.1. REPLACING SEALS AND CLEANING
9.3.2.1.1. VACUUM PUMP
The seal of the vacuum pump piston must be replaced after one year (or after 6 months if number of
samples / day > 30), to ensure proper functioning (stable and accurate vacuum counting value).
Picture 9-1: Vacuum pump assembly with the rod Picture 9-2: the rod
a) Disassemble the bearings bracket, by unscrewing the 3 screws, which allow you to take out
easily the rod and the piston from the block (1, 2 and 3).

Pictures 9-4
Pictures 9-3
b) Unscrew the 3 screws as shown on the picture,
Here is the mechanical assembly remove the bearing bracelet.
alone, with the bearing bracket.
Pictures 9-5
c) Take out the tubes from the vacuum pump, push up gently the rod and the piston to take them
out from the block.
Pictures 9-6

Pictures 9-7
d) Disassemble the parts to clean.
e) Clean the vacuum pump piston with an ultrasonic washing device or distilled water.
Picture 9-8

Picture 9-10: Partial vacuum pump assembly
Picture 9-9: Rod S0200XXX
f) Clean the rod and the gear from old grease g) Unscrew the 4 Screws on the top of the
and lubrifiate again. block
Picture 9-11: Vacuum pump housing and the seal Picture 9-12: Vacuum piston seal S020033
h) Take out the seal from the block and clean the vacuum pump with ultrasonic washing device
or distilled water.
i) Replace the seal with a new one. Re-assemble the parts and respect the order of assembling.
Be careful with the sense of the seal when you replace it

9.3.2.1.2. DILUTION BLOCK
The seals of the dilution block must be replaced after one year (or after 6 months if number of samples
/ day > 30), to ensure the proper functioning.

Pictures 9-13 and 9-14
a) Remove the retaining Teflon bracket, by unscrewing the five screws.
b) Take out gently the two pistons (Lyse piston and Diluent piston) from the block by pushing
down the metal part to create a space and the pistons for disassembling from the block.
c) Unscrew the five screws to remove Teflon bracket covering seals and O-ring.
d) Pull out the sample piston from its chamber.
e) Use the sample piston to turn the cable gland. “a quarter turn!”

f) Take out the Diluents and Lyse seals from the block.
g) Replace the seals with new spare parts.
Diluent piston seal; item code: Lyse piston seal; item code: O-ring sample piston; item
S020032 S020030 code: S020034
Pictures 9-21, 9-22 and 9-23
h) Clean all the parts of the dilution block by using the cleaning ultrasonic device or any equivalent
cleaning equipment. Re-assemble the sample piston as shown.

Picture 9-24
MANDATORY: respect the de-assembling / assembling order.

9.3.2.1.3. COUNTING CHAMBER
In case the counting chamber needs to be cleaned (PLT background or carry over issue), you have to
follow the bellow procedure.
a) Unscrew the screw to disconnect the electrical grounding wire (black cable) from the chamber
(not forget to disconnect also from the CCU Board)
b) Unscrew screws behind to desassemble the block.

c) Check the integrity of the chamber (no cracks in the plastic).
Pictures 9-31 & 9-32
d) Clean the RBC chamber using an ultrasonic device.

e) Clean the ruby and the silicon spacers with the ultrasonic device or SFRI Clair 3.3.
Pictures 9-33: Ruby Pictures 9-34: Silicon spacers
CAUTION: there is only one correct way to place the ruby. Please refer to draft bellow.
Picture 9-35
9.3.2.1.4. WASHING HEAD
The seal of the washing head must be replaced after one year (or after 6 months if number of samples
/ day > 30), to ensure the proper functioning.

Picture 9-36: Definition drawing of the washing head assembly
a) Take out the tubes from the washing head (2 tubes) and unscrew the two screws on the top
of the washing head.
b) Gently push down the washing head, to take it out from the sample probe.

c) Disassemble the top part from the block to take out the seal from the washing head.
Picture 9-37: Washing probe assembly
Picture 9-38: Washing probe assembly, exploded view Picture 9-39: O-ring probe
Washing head, item code:
S020034
d) Clean the parts of washing head with ultrasonic device.
Pictures 9-40 & 9-41
e) Replace the O-Ring with a new one.

9.3.2.1.5. VALVES
In case any valve needs to be cleaned and/or checked internally.
TWO WAY VALVE:
a) Unscrew the two screws and take out

the two flat scals.
b) Now, we can separate the valves from

its base. The spiral 4 inside the
component 3 may follow the
disassembly of the valves, do not forget
it.
Picture 9-43

c) Disassemble the component 3 from the
seal 2. Do not disassemble the seal.
Picture 9-45:Detail of the component 3 Picture 9-46
When the disassembly is done, you must obtain the same number of components as shows the
picture.

Picture 9-47
THREE WAY VALVE:
Same as above, just pay attention to the fact that the third way is an independent component of the
whole three ways valve system. Refer to the following picture.
Picture 9-48

10. DISASSEMBLING/RE-ASSEMBLING
FOR YOUR SAFETY, BEFORE THE ASSEMBLY OR DISASSEMBLY OF ANY PART OF THE
MACHINE, DON’T FORGET TO SHUT DOWN COMPLETELY THE COMPUTER, TO PUT THE
POWER SUPPLY SWITCH ON “O” POSITION AND TO UNPLUG THE MACHINE.
10.1. Removing panels
Picture 10-1
Picture 10-2
a) There are six M2 screws on the back of the machine. Unscrew them to take out the three
panels.
b) You can slowly slide each of them off.
Picture 10-3

Picture 10-2
Picture 10-3: View of the CCU board

Picture 10-4: View of the fluidic side
10.2. The CCU board

a) Unplug all the connections of the board, without forgetting the ones behind it.
Picture 10-4

Picture 10-5
b) Unscrew all the screws on the board, and take out the board.
Picture 10-6
10.3. The Fluidic Assembly

BE CAREFUL WHEN YOU WANT TO DISCONNECT THE TUBES FROM THE VALVES, PUMP ETC… A LITTLE
QUANTITY OF LIQUID COULD ESCAPE FROM IT. WEAR GLOVES DURING THE PROCESS.

10.3.1. VALVES
a) Take out the screws from the façade to disconnect the valves assemblies.
Picture 10-7
b) To disassemble completely the

valves, unscrew the other screws
from the support, and refer to
the page 89 for more
explanations of the two and
three ways valves systems.
Picture 10-8

10.3.2. DILUTION BLOCK
a) Unscrew the four screws in the dilution

block, and the two ones on the fixing plate
below. Be careful to maintain with the
other hand the dilution block system, for
it not to fall.
b) Take out the dilution block. Refer to the

page 81 for more explanations about the
disassembly of the dilution block
assembly.
Picture 10-10
c) Take out the plate slowly by maintaining

the position of the pistons.
Picture 10-9
Picture 10-11

10.3.3. VACUUM PUMP
a) Unscrew the four screws of the vacuum

pump base, and the one maintaining
the mechanical system which tracts the
piston.
b) Take out the vacuum pump. Refer to

the page 41 for more explanations
about the disassembly of the vacuum
block assembly.
Picture 10-12
Picture 10-13

10.3.4. COUNTING CHAMBER
Follow all the instructions of the disassembly described page 91.
Picture 10-14
10.3.5. MECHANICAL ASSEMBLY
Unscrew all the screws. For more details, refer to the page 84.

10.3.6. DISASSEMBLING THE SAMPLE NEEDLE MECHANICAL SYSTEM
Here is the mechanical system for the sample probe.
Picture 10-1
5
a) Take out the two circlips shown below.
Pictures 10-16, 10-17, 10-18 and 10-19

b) Unscrew all the screws and take out the
motor. You can disassemble the pulley-belt
system.
c) As shown on the picture below, unscrew

the two screws maintaining the slide.
Picture 10-20
Picture 10-23

d) For the horizontal guidance system, you have to take off all the fixing elements as screws or
circlips, same as above. Then, you will be able to disassemble the second pulley-belt system.
Picture 10-24
Picture 10-25

11. Troubleshooting
This chapter gives instructions for identifying, troubleshooting, and correcting instrument problems. If
you cannot solve the problems according to this guidance or you wish to get more information, please
contact your local distributor.
11.1. Error / Warning codes list

No. Alarm show Description
1 L Test result lower than reference lower limit
2 H Test result higher than reference higher limit
3 **.* Test result is invalid
4 HGB Bubble or failure HGB Blank voltage abnormal
5 BUBBLE Bubble in test
6 CLOG Sample chamber aperture clog
7 R1 Lymphocytes regional on the left abnormal
8 R2 Between lymph cell and middle cell regional abnormal
9 R3 Between middle cell and granulocyte cell regional abnormal
10 R4 granulocyte regional on the right abnormal
11 RM Much area exists abnormal
12 PM Platelets and red blood cells junction area abnormal
11.2. Background test abnormal

No. Possible cause Solution
1 The power supply voltage instability or share power Connect the power supply voltage stabilizer
2 Environment noise Good grounding connection
3 The temperature of reagent low Improve reagent temperature
4 Diluent pollution Replace diluent
5 Diluent block dirty Clean the diluent block
6 Sample needle can’t clean completely Replace seal washer
7 Sample chamber dirty Clean chamber
8 Sample chamber broken Replace sample chamber
9 2-way valve dirty or air leakage Replace 2-way valve
10 CCU board broken Replace CCU board

11.3. No diluent
1 Diluent tube bent Check diluent liquid road
2 No diluent Replace diluent
3 Diluent seal washer broken Replace diluent seal washer
4 Liquid sensor failure Replace liquid sensor
5 3-way valve leakage Replace 3-way valve
6 Liquid detection board voltage wrong Adjust the voltage to 3-5v
11.4. No cleaner
1 Cleaner liquid tube bent Check the cleaner liquid tube
2 No cleaner Replace cleaner
3 Cleaner liquid sensor failure Replace cleaner liquid sensor
5 The voltage of liquid detection board abnormal Adjust voltage to 3-5 V
11.5. No Lyse
1 Lyse tube bent Check lyse tube
2 No lyse Replace lyse
3 Lyse seal washer broken Replace seal washer
4 Liquid sensor failure Replace liquid sensor
6 The voltage of liquid detection board abnormal Adjust voltage to 3-5 V
11.6. HGB bubble or failure

1 Didn’t use original reagent Use original reagent
2 Sample chamber dirty Clean sample chamber
3 Sample chamber bubble inside Clean sample chamber
4 HGB blank voltage abnormal Adjust HGB voltage to4.31±0.02v
5 HGB data line broken Replace sample chamber

11.7. HGB value low
1 Calibration factor low Re-calibrate
2 Lyse not enough Prime lyse
3 HGB lamp broken Replace sample chamber
11.8. Bubble
1 Sample chamber bubble inside Exclude bubble
2 Add reagent too less Re-prime reagent
3 Set up count time too long Set up the count time again
4 Sample aperture broken Replace aperture
5 Count channel leakage Replace part of leakage tube
6 Between back cover and sample chamber Reassemble sample chamber back
leakage cover
7 2-way failure Replace 2-way valve
11.9. Clog
Negative pressure not enough Replace the vacuum pump seal washer
1 Replace gear
Replace Vacuum pump rack
2 Chamber aperture clog Clean sample aperture
3 The liquid road of detection unit broken or loose Check tube road
4 2-way valve clog Replace 2-way valve
5 MTB board Replace MTB board
Chamber wiring broken, faulty connection to Connect properly the chamber,
CCU, defective CCU. In that case the hole voltage Replace the chamber.
6
value is 28,50, which is not a real value, it Replace CCU board.
indicates that there is an open circuit.

11.10. PLT high
1 The power supply voltage instability or share power Connect the power supply voltage stabilizer
2 Environment noise Good grounding connection
3 The temperature of reagent low Improve reagent temperature
4 Diluent pollution Replace diluent
5 Diluent block dirty Clean the diluent block
6 Sample needle can’t clean completely Replace seal washer
7 Sample chamber dirty Clean chamber
8 Sample chamber broken Replace sample chamber
9 2-way valve dirty or air leakage Replace 2-way valve
11 Calibrate factor of PLT too high Adjust the calibrate factor of PLT.
11.11. PLT low

1 Anticoagulant not enough Replace qualified consumables
2 Calibrate factor wrong Re-calibrate
11.12. WBC high

1 Sample needle dirty Clean sample needle or replace seal washer
2 No lyse Replace lyse
3 Lyse seal washer become small Replace seal washer
4 V9 valve failure Replace v9 valve
5 Sample chamber dirty Clean sample chamber
6 Sample chamber failure Replace sample chamber
7 CCU board failure Replace CCU board
8 Calibrate factor of WBC too high Adjust the calibrate factor of WBC.
11.13. Instrument initialization failed

1 CF card interface loose Re-connect CF card
2 CF card broken Replace CF card
3 CCU board power interface loose Re-connect CCU board power supply line

11.14. Boot up white display
1 LCD cable loose Re-connect LCD cable
2 LCD cable failure Replace LCD cable
3 Display failure Replace display
4 CPU board power interface loose Re-connect CPU board power supply line
5 CPU board broken. Replace CPU board
6 Power supply failure Replace power supply

Appendices-TSB
Appendix 1 Safety Symbols ................................................................................................................ 111

Appendix 2 Reagents and Consummables list.................................................................................... 113
Appendix 3 Installation Check List ...................................................................................................... 115
Appendix 4 Preventive Maintenance Check List ................................................................................ 117
Appendix 5 Spare Part List.................................................................................................................. 121
Appendix 6 Preventive Maintenance Kit Content .............................................................................. 123

Appendix 1
Safety Symbols
SYMBOL WARNING CONDITION ACTIONS

Biohazard: Consider all materials Wear standard laboratory attire,
(specimens, reagents, controls, glove and follow safe laboratory
calibrators, or components that contain procedures when handling any
or have contacted human blood) as being material in the laboratory.
potentially infectious.
Electrical Shock Hazard: The electrical Before continuing, unplug the BA-88
warning icon alerts users to the analyzer from the electrical outlet.
possibility of electrical shock in the
described activity or at the posted
location.
Probe Hazard: The probe may contain Avoid any unnecessary contact with
biohazard materials, including controls the probe and probe area.
and calibrators.
NOTE: Indicates to the operator
information of importance in the
procedure to be performed. This heading
is also used to indicate specific sample
handling techniques that are helpful in
operating the instrument.
CAUTION: Indicate to the operator
information of importance that could
result in damage to the instrument or
affect the test due to improper operation
if these instructions are not followed.
WARNING: Indicate to the operator
information regarding potential hazards
that may cause personal harm to the
operator if these instructions are not
followed.

Appendix 2
Reagents and Consummables list
Reagents
REAGENTS REFERENCE VOLUME
SFRI Diluent 3.3 HSL324 20 liters
SFRI Lyse 3.3 HSL304 0,5 liters
SFRI Detergent 3.3 HSG304 2 Liters
SFRI CleanerEZ 5.3 (Conc. Cleaner) HSC304 60 mL
Strong Cleaner HSC104 60 mL
Blood controls and calibrator

NAME REFERENCE QUANTITY
BloodTrol 16, Normal Level R021005 3 x 3 mL
BloodTrol 16, LNH* levels R021001 3 x 3 mL
BloodTrol 16, Normal Level R021002 6 x 3 mL
BloodTrol 16, LNH* R021006 6 x 3 mL
* : LNH means Low, Normal, High
Blood calibrator
NAME REFERENCE QUANTITY
BloodCal R021003 1 x 3 mL

Appendix 3
Installation Check List
HEMIX 3-30
INSTALLATION CHECK LIST
Customer : Date :
Serial Number :
Environement
Item to check Range
Room temperature 18°C to 35°C
Humidity ≤ 70%
Atmospheric Pressure 86.0 kPa to 106.0 kPa
Avoid direct sunshine
Avoid dusty atmosphere
Check Bench dimensions
CheckBench supported weight
Power Supply
Item to check Range

Power Supply 110 - 220 Vac +/- 10%
50 - 60 Hz
Power Consumption 150 VA
Installation

Remove tie-wrap blocking the arm
Remove protecting tape from counting chamber
Connect reagents with supply external tubes
Connect the equipotential cable to an equipotential point
Connect the PS2 mouse and PS2 Key board
Starting
Start-up self test ok
Re-prime Lyse to avoid Lyse empty warning message
Run a few background to remove any micro-bubbles
Get 5 backgrounds within specs
Perform a CV with normal range SFRI blood control

(15 replicates, get the CV from factory mode)
Check if the results are in range
Calibrate if requiered with SFRI blood calibrator

(Never calibrate a non
stable system)
Training
Train all the users to routine operation
Train all users to periodic maintenance
Report
Send back to SFRI all requested documents
to start warranty support
SFRI Distributor Name Lab Name
Technician Name Customer Name
Signature and Stamp Signature and Stamp

Appendix 4
Preventive Maintenance Check List
PREVENTIVE MAINTENANCE GUIDELINES

HEMIX 3-30 PM Checklist
Customer : Date :
Serial Number :
GENERAL CHECK
1/ Check for latest User Manual presence

Report its version : _________________
2/ Report the current software version
________________________
3/ Check for User Maintenance proper execution
Perform and train end user if required
4/ Check for external / internal cleanliness, clean as required
Particularly the external air filter (user maintenance)
5/ Ask the user for last error messages
Investigate consequently and document in report
6/ Run #5 backgrounds
Check if the results are within specs, print and attach
7/ Perform the CV before maintenance procedure
sfri blood control, fifteen replicates
Print and attach
MECHANICS CHECK
1/ Clean and lubricate Probe axis (vertical movement - Motor A)
Use PM kit grease if available or similar, clean with tissu paper and acetone
2/ Clean and lubricate Probe Arm axis (horizontal movement - Motor B)
Use recommended grease, clean old grease with tissu paper and acetone
3/ Clean and lubricate Dilution Block axis (Motor C)
4/ Clean and lubricate Vacuum / Pressure Pump Block (Motor D)

5/ Clean / Replace air entry fan filter (rear panel)
Remove dust, replace if necessary
6/ Make sure the grounding nut on CCU board is tight
FLUIDICS CHECK
1/ Check all tubings (integrity, cleanliness, sealing…) and fittings
(replace any tube, valve or fittings as required)
2/ Check for any foreign body presence in the counting chamber
Remove and clean as required
3/ Check if the chamber is not cracked
Replace if necessary
4/ Check sample probe integrity
Replace if necessary
FLUIDICS CHECK… Continue…

5/ Check proper reagent priming
SPARE PARTS REPLACEMENT

1/ Replace spare parts included in PMKIT
Part number PMK0212…
Refer to procedure...
Replacing Lyse, Diluent seals
Replacing sample piston o-ring
Replacing vacuum pump seal
Replacing Lyse tubing line
ADJUSTMENTS AND CONTROLS

1/ Check all valves for proper commutation
You should hear the commutation sound
2/ Check HGB blank value : 4,31 +/- 0,2 Vdc
Adjust as required
3/ Vacuum Pump Negative Pressure : 256 mbar
Report Value ___________
4/ Vacuum Pump Leak rate : ≤ 2 mbar / 2 min
Report Value ___________
5/ Power Supply
Measure with voltmeter on CCU board Power Supply connector
+12 Vdc ___________ Vdc
+5 Vdc ___________ Vdc

6/ Diluent Temperature
Should match sample cup t°, ± 5°C
7/ Aperture Voltage Reference Value
Mean of 5 background replicates (Factory mode)
W.H.V.S. R.H.V.S.
1
2
3
4
5
MEAN
8/ Check Burn Function

Use voltmeter AC measurment in chamber
9/ Check Reagents Sensors Voltages
Use voltmeter DC measurment on Distribution Board
PERFORMENCES CHECK
1/ Run #5 backgrounds
Check if the results are within specs, print and attach
2/ Perform the CV before maintenance procedure
sfri blood control, fifteen replicates
Print and attach
3/ Calibrate if require
Attach the new calibration factors

Appendix 5
Spare Part List
Item Code Item Designation

FLUKE_177 Multimeter, Fluke 177
PCE-P15 Manometer, digital, PCE-P15
PMK0212 Preventive Maintenance Kit HEMIX 3-30
S020001-03 CPU Board V2 6154 (w/o memory stick w/o CF card)
S020002-04-EN Disc On Module HEMIX 3-30, English
S020002-04-FR Disc On Module HEMIX 3-30, French
S020002-04-SP Disc On Module HEMIX 3-30, Spanish
S020003-03 CCU Board, HEMIX 3-30
S020005-02 Optical Coupler Assy, Mechanical Home Sensor (V2)
S020007-02 2 Way Valve (HEMIX 3-30)
S020008-02 3 Way Valve (HEMIX 3-30)
S020009-02 Counting Chamber Assy, HEMIX-3-30
S020013-02 Reagent Detection Sensor (V2, Optical)
S020019 Burn Transformer (220 Vac - 110 Vac)
S020021 Stepper Motor
S020022 Vacuum pump Assembly
S020023 Dilution Machine
S020024-01 Dilution Block - New
S020025 Sample Probe
S020025-01 Sample Probe - New
S020026 Sample Probe Washer Assy
S020026-01 Sample Probe Washer Assy - New
S020028 Thermal Printer Assy
S020029 Start Switch Assy
S020030 Lyse Piston Seal
S020032 Diluent Piston Seal
S020033 Vacuum Piston Seal
S020034 O-ring (1,4 x 1,52)
S020034-01 Sample Probe Washer O-ring, New (Pkg x 5)
S020035 LCD Display Assy
S020040 Fuse T3,15A / 250 V / 5 x 20 mm (X10)
S020049-01 Diluent connection tube (complete line)
S020050-01 Lyse connection tube (complete line)
S020051-01 Rinse connection tube (complete line)
S020052-01 Waste connection tube (complete line)
S020053 Metal braket with bearings for pumps

Item Code Item Designation
S020054 Vacuum Pump Gear
S020054-01 Vacuum Pump Bearings Bracket
S020055 Driver Belt
S020056 Switching Power Supply Assy
S020058 Tygon Tube S50HL 2,4 x 4 mm
S020059 Tygon Tube S50HL 1,6 x 3,2 mm
S020060 Sample Piston O-ring, Old Model (2 x 1.8 mm)
S020061 SDRAM for CPU Board V1
S020062-02 Sample Machine (V2)
S020063-03 Distribution Board (HEMIX 3-30)
S020064 Vacuum Pump Machine
S020065 Rod for Vacuum Pump
S020066 Rod for Dilution Machine
S020067 LCD Adapter Board
S020068 TFT Cable CPU 615X
S020071 Inverter Power Supply Delay
S020072 PS2 QWERTY Keyboard
S020073 High Voltage Inverter Board
S020074 Tygon Tube S50HL 2,4 x 4 mm - 15 m roll
S020075 Tygon Tube S50HL 1,6 x 3,2 mm - 15 m roll
S020077 Waste Tool - BNC Load 50 ohms
S020078 Clavier AZERTY PS2
S020079 RS232-Crossed Connection Cable
S020080 USB to DB9 Serial Adapter Cable
S020081 PS2 Mouse
S020082 Cable / Distrib. Board to CCU - Motors and sensors
S020083 Cable / Distrib. Board to CCU - Reagent Sensors
S020084 Cable / Distrib. Board to OSB, OSC, OSD
S020085 Cable / Distrib. Board to OSA
S020086 Cable / Distrib. Board to Reagent OS
S020087 Cable / Distrib. Board to Motors
S020088 Counting Chamber Holder
S020089 Temperature Sensor

Appendix 6
Preventive Maintenance Kit Content
HEMIX3-30 PM Kit (p/n PMK0212) content

Part Number Designation Qty
HSC101 Hemaclair 1
HSC301 Diluclair 1
S020030 Lyse piston seal 1
S020032 Diluent piston seal 1
S020033 Vacuum piston seal 1
S020034* Sample needle washer O-ring (1,4 x 1,52) 1
S020050-01 Lyse connection tube (Complete line) 1
S020058*** Tygon tube S50HL, 2.4 x 4 mm (1 m) 1
S020059**** Tygon tube S50HL, 1.6 x 3.2 mm (1 m) 1
S020060** Sample piston O-ring, old model (2 x 1.8 mm) 1
*: Starting from s/n 0201XXXX, part is different, p/n is S020034-01

**: Starting from s/n 0201XXXX, part is different, p/n is S020034 same as old washer O-ring
***: Starting from s/n 0201XXXX, tube is different, p/n is S02XXXX
****: Starting from s/n 0201XXXX, tube is different, p/n is S02XXXX

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Revision History

Version Date Modification

HEMIX 3-30_Technical Manual_V15.00 | 3

1. GENERAL OVERVIEW ........................................................................ 15

2. INSTALLATION PROCEDURE ............................................................. 23

3. THEORY OF OPERATION ................................................................... 29

4. FLUIDICS SYSTEM .............................................................................. 31

HEMIX 3-30_Technical Manual_V15.00 | 5

6 | HEMIX 3-30_Technical Manual_V15.00

10. DISASSEMBLING/RE-ASSEMBLING ............................................. 93

HEMIX 3-30_Technical Manual_V15.00 | 7

11. Troubleshooting ........................................................................ 103

8 | HEMIX 3-30_Technical Manual_V15.00

Content of this manual is subject to changes without prior notice.

Responsibility on the Manufacturer Party

HEMIX 3-30_Technical Manual_V15.00 | 11

Workmanship and Materials

Safety, Reliability and Performance

12 | HEMIX 3-30_Technical Manual_V15.00

HEMIX 3-30_Technical Manual_V15.00 | 13

1.1. Front View

HEMIX 3-30_Technical Manual_V15.00 | 15

16 | HEMIX 3-30_Technical Manual_V15.00

Picture 1-3 : Front view of the fluidic side

Picture 1-4 : 3D View of the complete fluidic side assembly

HEMIX 3-30_Technical Manual_V15.00 | 17

18 | HEMIX 3-30_Technical Manual_V15.00

Picture 1-7 : 3D view of the internal part of the front panel

HEMIX 3-30_Technical Manual_V15.00 | 19

1.4. System Specifications

20 parameters and 3 histograms

1.4.1.1. MEASURED PARAMETERS

1.4.1.2. PARAMETERS GIVEN BY HISTOGRAM

1.4.1.3. CALCULATED PARAMETERS

20 | HEMIX 3-30_Technical Manual_V15.00

Sample (µL) Diluent (mL) Cleaner (mL) Lyse (mL)

Venous Capillary Pre-Diluted

Electrical input x 1 External VGA x 1

1.4.5. BLANK TEST

Parameter Result range Data range

1.4.6. CARRY OVER

Parameter Pollution range

HEMIX 3-30_Technical Manual_V15.00 | 21

Parameter Repeatability (CV %)

1.4.8. LINEARITY AND PRECISION

Parameter Linearity Range Parameter Linearity Range

22 | HEMIX 3-30_Technical Manual_V15.00

2.1. Pre-Installation on Site Inspection

2.1.2. LOCATION REQUIREMENTS

2.1.3. POWER REQUIREMENTS

2.2. Pre-Installation Instrument Inspection

2.3. Unpacking / Installing the HEMIX 3-30

HEMIX 3-30_Technical Manual_V15.00 | 23

24 | HEMIX 3-30_Technical Manual_V15.00

1: Sample needle mechanical Assy

Picture 2-2: Fluidic side

Picture 2-4: 3D View of the tie Wrap

Picture 2-3: photography of guidance

HEMIX 3-30_Technical Manual_V15.00 | 25

Picture 2-6: 3D View of the location of the tape

l) Place the reagents near the instrument.

DO NOT INCREASE THE LENGTH OF ANY OF THE REAGENT PICKUP TUBE

n) Connect the waste tube assembly to the back of the HEMIX3-30.

Sample 1.63.5mmPVC+Temperature 60+Temperature Dilution

2.44.2mmPVC+Straight 35+Straight Dilution

G6 V8-NO 1.63.5mmPVC 210 V9-C

G7 V10-NO 2.44.2mmPVC+DLS+2.44.2mmPVC 160+DLS+200 DLS(green)

G9 V7-NO 2.44.2mmPVC+LYS+2.44.2mmPVC 130+LYS+200 LYS(red)

G10 V3-C 2.44.2mmPVC 140 glass blub-1

G11 V3-NC 2.44.2mmPVC 240 Vacuum-1

G13 V2-C 2.44.2mmPVC 160 Vacuum-2

G15 V9-NC 2.44.2mmPVC 40 Tee-tube

G16 V4-NC 2.44.2mmPVC 35 Tee-tube

G17 V4-C 2.44.2mmPVC+CLS+2.44.2mmPVC 135+CLS+115 CLS（blue）

G18 Tee-tube 1.63.5mmPVC 65 steel tube‐1

G19 V11-NC 2.44.2mmPVC 130 glass blub‐2

G20 V11-C 2.44.2mmPVC 95 Vacuum‐4

G23 V6-C 2.44.2mmPVC 75 Vacuum‐6

G26 Chamber-2 1.63.5mmPVC 65 steel tube-3

G29 Glass bulb 1.63.5mmPVC 105 Tee-tube

G30 \ 2.44.2mmPVC 50 steel tube-3