Alur Identifikasi Mikroba Patogen (Salmonella sp.
Water Soluble Products
Make a 1 in 10 dilution in NaCl-
Peptone Solution pH 7, PBS pH 7,2
or Soybean Casein Digest Broth
Adjust to a pH of 6-8 if necessary
Nonfatty Products Insoluble in
Water
Make a 1 in 10 dilution in NaCl-
Peptone Solution pH 7, PBS pH 7,2
or Soybean Casein Digest Broth
Add a surfactant such as 1 g/L
Polysorbate 80. Adjust to a pH of 6-
8 if necessary
Fluids or Solids in Aerosol
Fatty Products Transdermal Patches
Form
Dissolve in filtered isopropyl Remove the protective cover
Move the sample aseptically to a
myristate or he minimum necessary sheets, place the adhesive side
sterillized container. use a defined
quantity of sterile polysorbate 80 or upwards on sterile plastc trays.
number of metered dose from each
another noninhibitory sterile cover the adhesive with sterile
container tested
surface-active gauze
Transfer patches to a suitable
if necessary heat them in <40°C. volume of the chosen diluent
make 1 in 10 dilution in a containing inactivators (polysorbate
prewarmed of chosen diluent 80 and/or lecithin). shake
vigorously for 30 minutes
 Use the quantity not less than 10 g or 10 mL to inoculate a
suitable amount of Soybean-Casein Digest Broth

Mix, and incubate at 30°C-35°C for 18-24 hours

Transfer 0,1 mL to 10 mL Rappaport Vassiliadis Salmonella

Enrichment Broth

Incubate at 30°C-35°C for 18-24 hours

Subculture on Xylose Lysine Deoxycholate Agar Plate

Incubate at 30°C-35°C for 18-24 hours

The possible presence of Salmonella is indicated by the growth of well-developed, red colonies, with or
without black centers. This is confirmed by identification tests.
The product complies with the test if colonies of the types described are not present or if the confirmatory
identification tests are negative.