Relative Expression

Relative Expression
A.

E.

0
1
2
3
0.0
0.5
1.0
1.5
2.0
C.

G.
#1 #2
5
Pr
#1 Pre
5 #2 e % of total Non B/Non T cells tSNE2
Po Po

Day:
Post
Pre
#2 st #1 st

0
20

-20

0
20
40
60
4 4
#2 Pre #1 Pre
4 4

CCR6
CCR6
Po Po

tSNE1

#2
st st

-20

19
Relative Expression Relative Expression

0
1
2
3
4
5
#2

3.0
3.5
4.0
4.5
5.0
5.5
#1

#14
Pr
5 #2 e
#1 Pre Po
5 #1 st
Po
4

59
#2 st

*
CCR4
4 #1 Pre
4

#15
0

*
#2 Pre Po

Cluster ID
4

CCR4
Po st
st

Relative Expression

#24
Pre

0
1
2
3
Relative Expression

110

Post
#2

2.0
2.5
3.0
3.5
4.0
4.5
Pr
#1 #2 e
5 Po
#1 Pre #1 st
5

*
Po 4 20
#1 Pre

*
CCR7
#2 st 4

*
4

203
Po

*
Pre-splenectomy (n=5)

#2 Pre

CD14
st
4
Po
st

Relative Expression
Relative Expression
tSNE2

241

0
1
2
3
4
0.00
0.05
0.10
0.15
0.20
0.25
F.
#1
-20
0
20

5 #2
#1 Pre Pr
5 #2 e
Po Po
tSNE1

#24
#15
#14
#2
#2 st

*
#1 st
4 4
-20

*
*
#2 Pre #1 Pre
4 4

CD123
CD16

CD11c
Po Po
st st
Tbet
-20

CD11c
Relative Expression
Relative Expression EOMES

0
1
2
3
4
5
Monocytes

0.0
0.5
1.0
1.5
Neutrophils

#1
5 #2
CCR7
0

#1 Pre Pr
5 #2 e
Dendritic cells

Po Po CD8a
0

#2 st st

*
#1

*
4 4 CD33

*
#2 Pre

CD33
#1 Pre

*
CD127
4 4
NK and Other cells

Po ICOS
D.

Po
st st
CD11b
Relative Expression CD27
Relative Expression
20
20

0.0
0.2
0.4
0.6
CXCR3

0.0
0.5
1.0
1.5
#1 #2
5 Pr
#2 e PD1
Post-splenectomy (n=5)

#1 Pre
5 Po
Po #1 st FoxP3

*
4

*
#2 st
4

*
#1 Pre CD45RO
4

PD-L1
HLA-DR

*
#2 Pre
4 Po
Po
st
st CCR4
CD123
Relative Expression
B.

Relative Expression CCR5 % of total Non B/Non T cells

0
1
2
3
4
5
0.0
0.5
1.0
1.5
2.0
2.5
#1
5 #2
Pr CD7
Pre-splenectomy (n=5)

#1 e Pr
5 e
0
20
40
60
80
100

#2

*
Po Po CD38
#2 st

*
#1 st

*
4 4 CD86

*
CD33
#2 Pre

*
#1 Pre
4 4

HLA-DR
Po
st
Po CCR6
st
Low

CD1c
-20

Relative Expression CD127

Relative Expression

0
1
2
3
4
5
#2 PD-L1

0.0
0.5
1.0
1.5
2.0
#1 Pr
5 #2 e
Po CD25
0

#1 Pre
5 #1 st

*
Po 4 CXCR5

*
#2 st #1 Pre

*
4 4
CD16

HLA-DR

CD56
Po

*
Protein Expression

#2 Pre
4 st
Po CD4
20

High

st
Pre

Colored by phenotype

CD56
Post

Post-splenectomy (n=5)