Laboratory Requisition Form - STUDENT

Title of experiment:

How does the rate of respiration, measured by the change in color of hydrogen
carbonate indicator through the UV Spectrophotometer, differ between
germinating chia, sunflower, pumpkin, flax and poppy seeds?

Submitted by: Madeline Choi Name of Mrs Gobin

(name of supervisor:
pupil)

Year / Group: Mobile 96462459

5BiSL1 (5TCT) no:

Date of 28/08/19 Email: madeline.choi@acsinternational.com.sg

submission:

Type of experiment: Science External Essay/Internal Assessment

Date required: Time /

Period:

Apparatus / Chemicals QUANTITY CONCENTRATION

Germinating chia seeds 75g

Germinating sunflower seeds 75g
Germinating flax seeds 75g
Germinating pumpkin seeds 75g
Germinating fennel seeds 75g
UV Spectrometer 1
Hydrogen carbonate indicator 500cm^3
solution
Stopwatch 1
Plastic vials with cover 25
Dropper 25
Cuvettes 25

Other Remarks / Requests:

 Risk Assessment
Work Description

Environmental Hazard
New Trial

Oxidizing/ Explosive
Repetitive Work

Health hazard
Flammable
(A) Hazard Identification –

Corrosive

Harmful
Services Used

Irritant
Toxic
Chemical Substances Electricity
Water
Name of Hazardous Substances/
Compressed Gas
Reactant/ Products Used Others (List Details) (e.g. N2(Liq.) , Butane Can)
_________________________________ ________________ Heating Flame
_________________________________ ________________ Oven
_________________________________ ________________ Spectrophotometer
_________________________________ ________________ Water Bath (Temp _________oC)
_________________________________ ________________

(B) Hazard Identification - Materials

Persons at Risk: New
Activity Done: Hazards Identified:
I.E.: Who could be Risk Factor: Actions taken to Risk Factor:
I.E.: What is taking place as I.E.: what can cause
harmed by the (High, Med or minimize risk: (High, Med
part of the event? harm?
hazard? Low) or Low)
Myself Turn off the gas once done,
E.G.: Using Bunsen burner Can cause fire or burn Medium Low
and others no flammable chemicals nearby

(C) Controls Adopted for Risk Minimization OFFICAL USE ONLY(by School):
1. Flammability 3. State health issues that may 5. Body Contact (D) Conclusion of Risk
Volume – Small/ Med/ Large impact your practical: Safety Goggles Assessment to Health
No Ignition Sources E.G. Skin Allergies, Eczema Face Shield No significant risk
Use in Fume Hood __________________________________ Gloves (no supervision required)
Temperature of Reaction __________________________________ Masks Significant risk but adequate controls
_______oC __________________________________ Lab Coats in place
Eye Wash High risk (need vigilant supervision)
2. Toxicity 4. Awareness of safety equipment location Safety Shower
Use in Fume Hood Safety Shower/ Eye Wash First Aid Box Proper Footwear Approved By:
Use in Laminar Flow Hood Fire Extinguisher Fire Blanket ____________________
(Name & Signature of Supervisor/Teacher)
Date: __________________________