SULIT 4551 / 3

Nama :
Biology
Kertas 3
MEI 2018
1 ½ jam Kelas :

______________________________________________
PEPERIKSAAN PERTENGAHAN TAHUN 2018
BIOLOGI KERTAS 3
TINGKATAN EMPAT
4551/3

Satu jam tiga puluh minit

JANGAN BUKA KERTAS SOALAN INI SEHINGGA DIBERITAHU

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Untuk kegunaan pemeriksa

1. Tulis nama penuh dan kelas anda
pada ruang yang disediakan.
Markah Markah
2. Kertas soalan ini adalah dalam Soalan
Penuh Diperoleh
dwibahasa.

3. Soalan dalam bahasa Inggeris 1 33

mendahului soalan yang sepadan
dalam bahasa Melayu. 2 17

4. Calon dibenarkan menjawab

keseluruhan atau sebahagian soalan Jumlah 50
anda sama ada dalam bahasa
Inggeris atau bahasa Melayu.

5. Calon dikehendaki membaca

maklumat di halaman belakang kertas
soalan ini.

Kertas soalan ini mengandungi 12 halaman bercetak

_________________________________________________________________________

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SULIT 4551/3
Answer all queation.
Jawab semua soalan

The effect of different concentrations of sucrose on the size of potato discs was determined
by carrying out the following steps:

Step I: Using a cork borer, 5 cylinders of potato tissue were obtained. Each cylinder was then
sliced to make 10 discs of uniform size.

Step 2: 10 discs were taken and measured as shown in Diagram 1.1

Kesan kepekatan sukrosa yang berbeza ke atas saiz dis kentang telah dikaji mengikut
langkah seperti berikut

Langkah 1: 5 selinder tisu kentang disediakan menggunakan penebuk kentang.setiap

selinder telah dipotong membentuk 10 keping kentang yang sama saiz
Langkah 2: 10 kepingan kentang diambil dan diukur seperti ditunjukkan dalam rajah 1.1

Diagram 1.1 / Rajah 1.1

Step 3: The discs were immersed in 100 ml of distilled water in a Petri dish labelled J.
Step 4: Steps 2 and 3 were repeated for the remaining discs which were then placed in Petri
dishes labelled K,L,M and N, each containing sucrose solutions as shown in
Table 1.1.

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Langkah 3: kepingan kentang telah direndam di dalam piring petri berlabel J berisi 100ml
air suling
Langkah 4: Langkah 2 dan 3 telah diulang dengan larutan sukrosa yang diletak di dalam
piring petri berlabel K, L, M dan N seperti ditunjukkan di dalam jadual 1.1

Petri dish/ piring petri Solution / Larutan

Distilled water
J
air suling
0.2 M sucrose solution
K
0.2M larutan sukrosa
0.4 M sucrose solution
L
0.4M larutan sukrosa
0.6 M sucrose solution
M
0.6M larutan sukrosa
0.8 M sucrose solution
N
0.8M larutan sukrosa

Table 1.1./ jadual 1.1

Step 5: After an immersion period of 30 minutes. the discs in each Petri dish were
measured.The results of the experiment are shown in Table 1.2.

Langkah 5: Selepas merendam selama 30 minit , kepingan kepingan kentang di dalam

piring petri diukur. Keputusan eksperimen ditunjuk di dalam jadual 1.2

Petri
The final length of I0 discs of potato after immersion for 30 minutes(cm) Panjang akhir
dish
10 keping kentang selepas direndam selama 30 minit(cm)
piring
petri

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Table 1.2 // Jadual 1.2

For
Examiner’s
Use
(a) Record the final length of the 10 discs for each of the solutions in the spaces provided in
Table 1.2. 1(a)
Rekodkan panjang akhir untuk 10 kepingan kentang untuk setiap larutan di dalam ruang

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yang disediakan dalam Jadual 1.2. [3 marks] [3

3
markah]

(b) (i) State two different observations made from Table 1.2.
Nyatakan dua pemerhatian yang berbeza yang dibuat daripada Jadual 1.2

Observation 1 // pemerhatian 1:

1(b)(i)
…………………………………………………………………………………………….……

………….………...…………………………………………………………………………… 3

Observation 2 // pemerhatian 2:

…………………………………………………………………………………………………..

……………………………………………………..……………………………………………
[3 marks]
[3 markah]

(ii) State two inferences which correspond to the observation in 1(b) (i)
Nyatakan dua inferens yang sepadan dengan pemerhatian di 1(b) (i)

Inference from observation 1 // inferen daripada pemerhatian 1:

…………………………………………………………………………………………………
1(b)(ii)

…………..………………………….…………………………………………………………

Inference from observation 2 // inferen daripada pemerhatian 2: 3

…………………………………………………………………………………………………

…………………………………………………………………………………………………
[3 marks]
[3 markah]
c) Complete Table 2 based on this experiment.
For
Lengkapkan Jadual 2 berdasarkan eksperimen ini Examiner’s
Use

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1(c) 3
SULIT 4551/3 (PP)

Variables Method to handle the variable

Pembolehubah Cara mengendalikan pembolehubah

Manipulated variable
Pembolehubah dimanipulasikan ……………………………………………

………………..…………………… ……………………………………………

………………………..…………… ……………………………………………

Responding variable
Pembolehubah bergerak balas ……………………………………………

1(c)
…………...………………………… ……………………………………………

……………………..…….………… ……………………………………………
3
Constant variable
Pembolehubah dimalarkan ……………………………………………

………………………...…………… …………………………………………..

……………………..……………… ……………………………………………

Table 2 // Jadual 2
[3 marks]
[3 markah]
d) State the hypothesis for this experiment.
1(d)
Nyatakan hipotesis bagi eksperimen ini.
……………………………………………………………………………………………...….

…..………………………………………….…………...………………………….………….
[3 marks] 3

[3 markah]

For
e) (i) Construct a table and record all the data collected in this experiment. Your Examiner’s
Use
table should have the following aspects:

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Bina satu jadual dan rekodkan semua data yang dikumpulkan dalam eksperimen ini.
Jadual anda hendaklah mengandungi aspek-aspek berikut:
 Concentration of sucrose solution/ Kepekatan larutan sukrosa
 Initial length of 10 discs/ Panjang asal 10 kepingan kentang
 Final length of 10 discs / Panjang akhir 10 kepingan kentang
 Difference in length of 10 discs/ Perbezaan panjang 10 kepingan kentang
 Percentage difference in length of 10 discs/ Peratus perbezaan panjang bagi
10 kepingan kentang

Percentage difference in length of 10 discs = Difference in length of 10 disc X 100

Initial length of 10 discs

Peratus perbezaan panjang bagi = Perubahan panjang 10 kepingan kentang X 100

10 kepingan kentang Panjang asal 10 kepingan kentang
[3 marks] 1(e)(i)
[3 markah]

(ii) Based on the result of this experiment , Plot a graph percentage difference in length
of 10 discs against the concentration of sucrose solution in the graph paper provided. 1(e)(ii)
Berdasarkan keputusan eksperimen ini, plot dan lukis graf peratus perbezaan panjang
bagi 10 kepingan kentang melawan kepekatan larutan sukrosa menggunakan kertas
3
graf yang disediakan. [3 marks] [3
markah]

Percentage difference in length of 10 discs against the concentration of sucrose solution

Peratus perbezaan panjang bagi 10 kepingan kentang melawan kepekatan larutan sukrosa

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f) Based on the graph that you have drawn in 1(e) (ii) , explain the relationship between the For
Examiner’s
Use
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concentration of the sucrose solution and percentage difference in length of 10 discs of

potato.
Berdasarkan graf yang dilukis, terangkan hubungan antara kepekatan larutan sukrosa
dan peratus perubahan panjang 10 kepingan kentang
1(f)
……………………………………………………………………………………….………

3
………………………………………..………………………………………………………………

…………………………………………………………………….……………………….…………

………………………………………………………………………………………………………..
[3 marks] [3
markah]

g) The experiment is repeated but this time each potato cylinder is sliced into 10 thicker
discs. The initial length of the 10 discs is 11 cm.
Predict and explain the final length of 10 discs of potato if they are immersed in distilled
water. 1(g)
Eksperimen diulangi tetapi tetapi setiap kepingan kentang telah dihiris tebal sebanyak
10 keping. Panjang awal 10 kepingan ialah 11 cm.
3
Ramal dan terangkan panjang akhir 10 kepingan kentang sekira ianya direndam di
dalam air suling

…………………………………………………………………………………………….………

…..………………………………………….…………...……………………………………….

……………………………………………………………………………………………………

……………………………………………………………………………………………………
[3 marks]
[3 markah]

For
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SULIT 4551/3 (PP)

(h) Based on the result of the experiment, state the operational definition for osmosis?
Berdasarkan keputusan eksperimen ini, nyatakan definisi secara operasi bagi osmosis

…………………………………………………………………………………………….……

…..………………………………………….…………...…………………………….………. 1(h)

……………………………………………………………………………………………….…
3

…………………………………………………………………………………………………
[3 marks]
[3 markah]

(i) Classify the following as material or apparatus as used in this experiment.

Klasifikasikan bahan atau radas yang digunakan dalam eksperimen ini

Distilled water, sucrose solution, petri dishes, measuring cylinder, ruler, potato

Air suling, larutan sukrosa, piring petri, silinder penyukat, pembaris, ubi
kentang

Material Apparatus
bahan Radas

1(i)

Total 1
[3 marks ]
[3 markah]
33
2.

The rate of enzyme reaction is influenced by temperature. When the temperature is low,

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the enzyme is not active. When the temperature increases, the enzymes are more active.
Enzymes worked best at the optimum temperature and become denatured if the
temperature is increased above the optimum temperature.

Kadar tindak balas enzim dipengaruhi oleh kesan suhu.Apabila suhu rendah, enzyme
tidak aktif. Apabila suhu meningkat, enzim semakin aktif. Enzim sangat aktif pada suhu
optimum dan mengalami denaturasi apabila suhu melebihi suhu optimum.

Based on this statement, design an experiment to show the effect of temperature on the rate
of reaction on the enzyme amylase on starch.
Berdasarkan pernyataan ini, reka bentuk satu experimen makmal untuk mengkaji kesan
suhu ke atas kadar tindak balas enzim amilase.

Plan your experiment based on the following aspects:

Perancangan eksperimen anda hendaklah meliputi aspek-aspek berikut:
 Problem statement/Pernyataan masalah
 Hypothesis/Hipotesis
 Variables/Pembolehubah
 List of apparatus and materials/Senarai radas dan bahan
 procedure of experiment /Prosedur ekperimen
 Presentation of data/Persembahan data
[17 Mark]
[17 Markah]

END OF QUESTION PAPER

KERTAS SOALAN TAMAT

PEPERIKSAAN PERTENGAHAN TAHUN 2018 4551/3(PP)

TINGKATAN EMPAT

Biology
Kertas 3
MEI 2018
1 ½ jam Satu jam tiga puluh minit

UNTUK KEGUNAAN PEMERIKSA SAHAJA

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AMARAN

Peraturan pemarkahan ini SULIT dan Hak Cipta Panitia Biologi.

Kegunaannya khusus untuk pemeriksa yang berkenaan sahaja.
Sebarang maklumat dalam peraturan pemarkahan ini tidak boleh
dimaklumkan kepada sesiapa. Peraturan pemarkahan ini tidak
boleh dikeluarkan dalam apa-apa bentuk media.

_________________________________________________________________________
Peraturan pemarkahan ini mengandungi 11 halaman bercetak

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Question 1
No Answer Score
1(a) Able to record all 5 readings for The final length of I0 discs of potato after 3
immersion for 30 minutes(cm)

Sample answer:
Petri dish The final length of I0 discs of potato
after immersion for 30 minutes(cm)

J 11.4
K 11.3
L 10.6
M 10.4
N 10.2
Able to record any four readings correctly. 2
Able to record any two to three readings correctly 1
None of the above or no response 0
1(b)(i) Able to state two different observations correctly.(both have 3K) 3

(Observation must have value for MV and RV)

MV - the concentration of sucrose solution
RV- The final length of I0 discs of potato after immersion for 30 minutes(cm)
Sample answer:
1. In distilled water, the final length of the 10 discs is the 11.4 cm
2. In 0.8 M sucrose solution, the final length of the 10 discs is the
10.2 cm.
Able to state one different observations correctly and one inaccurate 2
observations(have any 2K) or both different observation only have any 2K)

Sample answer:
1. In distilled water, the final length of the 10 discs is the 11.4.
2. In 0.8 M sucrose solution, the final length of the 10 discs is the
shortest

Able to state one inaccurate observations(have any 2K) and one at idea level 1
(only have 1K) or both at idea level (only have any 1K)
Sample answer:
1. When they are immersed in distilled water, the final length is
longest
2. The final length of the discs is the increased/increased/change

None of the above or no response 0

 Summary of scoring for observation and inferent:
Score Correct Inaccurate Idea Wrong
3 2 - - -

1 1 - -
2 - 2 - -

1 - 1
- - 2 -
1 1 - - 1
- 1 1 -

0 - 1 - 1
0 - - 1 1

No Answer Score
(b)(ii) Able to make two inferences correctly (have 1 K1 and 1 K2/k3) 3
K1- the concentration of sucrose solution is low//high
K2- the size of potato become big//small // the diameter of potato become
long/short
K3- water molecule diffuse in/out by osmosis
Sample answer:

1. when the concentration of sucrose solution is low ,the size of the potato
discs big because the water molecule diffuse in to discs by osmosis

2. when the concentration of sucrose solution is high ,the size of the potato
discs small because the water molecule diffuse out the discs by osmosis

Note : Inferences must match observation.

Able to make one correct inference and one inaccurate inference (only have 2
1K).

Sample answer ( Inaccurate ):

1. when the concentration of sucrose solution is low ,the size of the
potato discs increased
2. the size of the potato discs small because the water diffuse in to
the discs by osmosis.
Able to state two inaccurate inferences OR 1 inaccurate inference and/ 1
inference at idea level.

Sample answer:
1. the size of the potato discs increased/decreased
2. water move in /out into the discs by osmosis

None of the above or no response 0

(c) Able to state all 3 variables and the 3 methods to handle the variable. 3
Sample Answer:
Variable Method to handle the variable
Manipulated variable Use different concentration of
Concentration of sucrose solution (distilled
sucrose solution water,0.2M,0.4M, 0.6M and 0.8M )
Responding variable
Final length of 10 discs Measure and record Final
length of 10disc by using a ruler

percentage difference in Calculate and record the percentage

length difference in length using formula

Constant variable
Volume of sucrose solution Fix the volume of sucrose solution as
100ml
Initial diameter of potato disc Use the same diameter of potato disc

Able to state any 4 — 5 correct 2

Able to state any 2 — 3 correct 1
No response /I response or incorrect response. 0

No Answer Score
(d) Able to state a hypothesis relating the manipulated variable and the
responding variable correctly with 3P
3
P1 - manipulated variable – Concentration of sucrose solution
P2 - responding variable – Final length of 10 discs// difference
In length of 10 discs// Percentage difference
in length of 10 discs
P3 - relationship between P1 and P2.—increases/decrease and
increase/decrease

Sample answer :
As the Concentration of sucrose solution increases, the final length of
10 discs // difference In length of 10 discs// Percentage difference in
length of 10 discs decrease
 Able to state a hypothesis relating the manipulated variable and the
responding variable correctly with 2P
2
Sample answer :
1. – Concentration of sucrose solution influences the Final length of 10
discs
2. The size of potato disc affected by c oncentration of sucrose solution

Able to state a hypothesis relating the manipulated variable and the 1

responding variable correctly with 1P
None of the above or no response 0

(e((i) Able to construct a table correctly with 3K 3

K1 - Able to state the 5 titles with units correctly.
K2 - Able to record all the data correctly.
K3 - Able to calculate and record the data correctly

lnitial Final difference Percentage

Concentration, length length in difference in
of sucrose of 10 of 10 length of length of 10
solution discs discs 10 discs discs
(M) (cm) (cm) (cm) (cm)

0 11 11.4 0.4 3.64

0.2 11 11.2 0.2 1.82
0.4 11 10.6 -0.4 -3.64
0.6 11 10.4 -0.6 -5.46
0.8 11 10.2 -0.8 -7.27

Able to construct a table correctly with 2K 2

Able to construct a table correctly with 1K 1
None of the above or no response 0

(e) (ii) Able to draw the graph correctly with 3K 3

K1 - Correct title with unit on both horizontal and vertical axis

and uniform scale )
K2 – All points plotted correctly, as in the table
K3 – Able to draw a smooth graph with positif gradient
Able to draw the graph correctly with 2K 2
Able to draw the graph correctly with 1K 1
None of the above or no response 0
No Answer Score
(f) Able to interpret data correctly and explain with 3K 3

K1 – relationship between concentration of the sucrose solution and the

percentage difference in length of 10 discs

K2 – at high concentration of sucrose :the sucrose solution is hypertonic to

the cell sap of potato tissue // at low concentration of sucrose: the
sucrose solution is hypotonic to the cell sap of potato tissue

K3 - water diffused out// in potato tissue by osmosis

Sample answer:

When the concentrations of sucrose is increased, percentage difference in

length of 10 discs decreased, because at high concentration of
sucrose,the sucrose solution is hypertonic to the cell sap of potato tissue
and water diffused out to potato tissue by osmosis

When immersed at lower concentrations of sucrose, the percentage

difference in length of 10 discs increased because at low concentration of
sucrose solution, the sucrose solution is hypotonic to the cell sap of potato
tissue and water diffused in to potato tissue by osmosis

Able to interpret data correctly and explain with 2K 2

Able to interpret data correctly and explain with 1K 1
None of the above or no respons 0
 (g) Able to predict and explain the outcome of the experiment correctly 3
with 3K
K1 – The final length of the potato discs decreased/will be less than 11.4
cm (any value less than 11.4cm)
K2 – because the thicker discs have a smaller surface area to volume ratio,
K3 – the rate of diffusion of water into the discs the slow

The final length of the potato discs is 11.0 cm. This is because the thicker
discs have a smaller surface area to volume ratio, so the rate of diffusion of
water into the discs. the slow
Able to predict and explain the outcome of the experiment correctly with 2
2K
Able to predict and explain the outcome of the experiment correctly with 1
1K
None of the above or no response 0

(h) Able to define operationally based on the result of the experiment 3

with 3K
K1 – the diffusion of water molecule//water diffused in//out in to the disc of
potato
K2 – the different in length of 10 disc of potato
K3 – influence by the concentration of sucrose solution//hypothesis
statement

Osmosis is the diffusion of water molecule into the disc of potato and it
shows by the difference in length of 10 disc of potato. The diffusion of
water influence by the concentration of sucrose solution.

Able to define operationally based on the result of the experiment with 2K 2

Able to define operationally based on the result of the experiment with 1
1K// hypothesis statement
None of the above or no response 0

No Answer Score
(i) Able to classify correctly 3
6 correct – 3mark
Sample answer:

Materials Apparatus
Distilled water Petri dishes
sucrose solutions measuring cylinder
potato ruler

4-5 correct – 2 mark 2

2-3 correct – 1 mark 1

1 - None of the above or no response 0

 Question 2:

Aspect Mark scheme Marks

Able to state a problem statement base on the following criteria:

Problem
statement: P1: Effect of temperature
01 P2: Activity of the enzyme amylase //the rate of enzyme amylase 3m
reaction
3 mark P3: Relation between P1 and P2 in question form (question mark
included)

Sample answer:
What is the effect of temperature on the activity of the enzyme
amylase?

Able to state a problem statement base on any two the following criteria. 2m

Able to state a problem statement at idea level. 1m

Wrong response 0m
No
Able to state a hypothesis base on the following criteria:
Hypothesis
02 P1: temperature
P2: Activity of the enzyme amylase //the rate of enzyme amylase 3m
3 mark reaction// the time taken for the iodine stop change the colour//the time
taken for hydrolysis of starch.
P3: increase-increase//decreased-decreased

Sample answer:
When the temperature is increased, the rate of enzyme amylase
reaction is increased.

When the temperature is increased, the time taken for the iodine stop
change the colour is faster.

Able to state hypothesis base on any two the following criteria. 2m

Able to state a hypothesis at idea level. 1m

Wrong response 0m
No

Sample answer:
Variables Manipulated variables: temperature 1m

3 mark Responding variables: Activity of the enzyme amylase //the rate of 1m

enzyme amylase reaction// the time taken for the iodine stop change the
colour//the time taken for hydrolysis of starch.

Constant variables: volume of the starch solution //volume of amylase

 solution 1m

Able to write all the apparatus and materials listed correctly:

Apparatus 3m
Materials Apparatus: Beaker, test tube, thermometer, syringe, stopwatch,
(AM) Bunsen burner, tripod, wire gauze, dropper , glass rod, white tiles
Materials: 1% starch suspension, saliva / amylase suspension, 6A+ 5 M
05 water, ice cube, iodine solution 0r
Water bath &
3 mark compulsary: starch suspension, saliva / amylase suspension 3A + 4m

water bath = bunsen burner + wire gause+ tripod, beaker, thermometer,

water …. 3A + 1 m

Able to write at least 2m

4 – 5 A + amylase + starch + 1M

Able to write at least 1m

Any 3 A + amylase + starch // water bath + amylase + starch

Wrong response 0m
No
 Able to write all the following criteria: (5K)
Procedure
04 K1: Preparation of materials and apparatus. (any 4)- [no 1-7]
 Diagram must have at least 5 correct labels
3 mark  Use a syringe to put 5ml of 1% starch suspension into each of
the test tubes,
 Use a second syringe to put 2 ml amylase solution into each of
the test tubes,
 Immerse test tubes A1and A2, B1 and B2, C1 and C2 and D1
and D2, El and E2 into five different beakers contain water bath
 After 5 minutes of immersion, pour the starch suspension test
3m
tube A1 into test tube A2
 Start stop watch
 Use a dropper to put a drop of mixture from test tube A, onto the
iodine solution in the first groove of the white tile
 Record the data in the table

K2: Operating fixed variable. (any 1)- [n0 1 and 2]

 Fix the same volume of the starch suspension. (eg: 5ml of
starch suspension)
 Fix the same volume of amylase. (eg: 2 ml)

K3: Operating responding variable. (any 1) [no 8]

 Record the time taken for……………………

K4: Operating manipulated variable. (any 1) [no 3 and no10]

 five different beakers contain water bath with temperatures kept
constant at 0 °C, 28 °C, 37°C, 45 °C and 60°C.

 Repeat experiment with different temperature 28 °C, 37°C,

45 °C and 60°C.

K5: Precaution / Accuracy of experiment. (any 1) [no 7 and 9]

 Rinse the dropper after each sampling
 Rinse the mouth before collecting saliva
 Repeat experiment to get average reading

Able to write 3 to 4k only 2m

Able to write 2K only 1m

Able to write 1K only 0m

Wrong response 0m
No
5K = 3 M
3-4K = 2M
2K = 1M
1K = 0M
 Sample answer:
Procedure:
1) Use a syringe to put 5ml of 1% starch suspension into each of
the test tubes, labelled A1,B1, C1,D1, E1.
2) Use a second syringe to put 2 ml amylase solution into each of
the test tubes, labelled A2, B2, C2, D2 and E2
3) Immerse test tubes A1and A2, B1 and B2, C1 and C2 and D1
and D2, El and E2 into five different beakers contain water bath
with temperatures kept constant at 0 °C, 28 °C, 37°C,
45 °C and 60°C.
4) After 5 minutes of immersion, pour the starch suspension test
tube A1 into test tube A2
5) Use a dropper to put a drop of mixture from test tube A, onto the
iodine solution in the first groove of the white tile and start
stopwatch.
6) Repeat the iodine test for every minute for 10 minutes..
7) Rinse the dropper after each sampling.
8) Record the time taken for the completion the hydrolysis of
starch// the time taken for iodine solution to remain yellow.
9) Keep the test tubes with the mixture in their respective water
baths throughout the experiment
10) Repeat the other experiment with other temperature 28 °C,
37°C, 45 °C and 60°C.
11) Record the result in the table.

Result
Sample answer:
2 mark 2m

1 mark 1 mark

Test the time taken for the rate of enzyme

tube Temperature the iodine solution amylase reaction
(°C) to remain yellow (1/time)
(min)
A 0
B 28
C 37
D 45
E 60
 Total Mark 17

END OF MARKING SCHEME

PERATURAN PEMARKAHAN TAMAT