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Microbiology Lecture Others

 P – synthesis of nucleic acid

Nutrition, Laboratory Culture, and Metabolism of  S – amino acids, vitamins
Microorganism  K – enzymes
 Mg – stabilize ribosomes, membranes
Microbial Metabolism and its Importance  Ca – stabilize cell walls, heat stability
total of all chemical reactions in the cell and is (spores)
divided into two parts
 Na – for their habitat e.g. salt water
 Fueling reactions Reqs. for Carbon, Hydrogen, and Oxygen
Often satisfied together
 Energy-conserving reactions
 Carbon source provides H,O , and
 Provide ready source or reducing power
 Generate precursors for biosynthesis
 Use organic molecules as carbon
sources which often also serve as energy
 The synthesis of complex organic
molecules from simpler ones
 Can use a variety of carbon sources
 Requires energy from fueling reactions
Microbial Metabolism
 Use carbon dioxide as their sole or
 Microbes have representatives in all five
principal carbon source
major nutritional types
 Must obtain energy from other sources
 Contribute to cycling of elements in
 Prokaryotes that build all of their cellular
structures from CO2
Microbial Nutrition
 Metabolism – catabolism (energy
 Agents produced by cells to obtain iron
releasing) & anabolism (energy
from insoluble minerals; blind iron and
transport it into the cell
 Chemoorganotrophs – organic cpds for
carbon & energy
Nutritional Types of Organisms
 Macronutrients – elements required in Based on energy source
fairly large amounts, structure &
metabolism Majority of microorganisms known
 Micronutrients & growth factors –  Photolithoautotrophs (photoautotrophs)
metals & organic compounds needed in
 Chemoorganicheterotrophs
very small amounts
Microorganisms may change Nutritional Type
 Some have great flexibility based on
 Carbon – 50% cell, major elements
environmental requirements
required, Aa, fatty acids, organic acids,
sugars, N
Chemoorganic Fueling Processes – Respiration
 Nitrogen – (12%) 2nd most important,
 Iron – cellular respiration
 Aerobic
 Far higher yield of ATP
- Oxygen is present
- Then, glucose is oxidized
completely to Carbon dioxide

Bacterial Respiration
 Aerobic Bacteria (obligate aerobes)
- They require free oxygen to
carry out cellular respiration
 Obligate Anaerobes
- They do not require free oxygen
for cellular respiration
- They cannot live in the presence
of O2
 Facultative Anaerobes
- They can live in either the
presence or absence of O2