Determination of Fe (II)

Concentration A510
4.0 0.160
10.0 0.390
16.0 0.630
24.0 0.950
32.0 1.260
40.0 1.580

SLOPE 0.0394924
INT -0.0010079

Calculations

Absorbance 1
Concentration, ppm 25.346823

ERROR ANALYSIS

Sr(stand.error in y) 0.003346
N 6
Sxx 926
Summation Xi 126.0
Summation Xi^2 3572.0
Sm 0.00011
Sb 0.002683
406x - 0.0010079194
47

15.0 20.0 25.0 30.0 35.0 40.0 45.0

f(x) = 0.0394924406x - 0.0010079194

R² = 0.9999689947

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0

Concentration, ppm
 SINGLE STANDARD ADDITION

Concentration,ppm Volume, mL Absorbance

Standard 3.00 5
Analyte unk 25.00 0.276
Standard + Analyte 0.491

Unk (mg/L) 0.7702325581

volume aliquot 25
volume total diluted 500

dilution factor 20

conc in ppm (mg/L) 0.77023 analyte molar mass

conc in mol/L #DIV/0!

conc in g/L 0.0007702326 volume total analyte, mL 25.00

volume total sample, L 0.025

total mass sample, g 3.65

% Unk in sample 0.0105511309

 AxCsVs/
Note Cx= (Ax+s)VX
 SINGLE STANDARD ADDITION

Concentration,ppm Volume, mL Absorbance

Standard 2.75 5.00
Analyte unk 50.00 0.231
Standard + Analyte 0.549

Unk (mg/L) 0.1997641509

volume aliquot 0
volume total diluted 0

dilution factor #DIV/0!

conc in ppm (mg/L) 0.19976 analyte molar mass

conc in mol/L #DIV/0!

conc in g/L 0.0001997642 volume total analyte, mL 0.00

volume total sample, L 0

total mass sample, g 3.65

% Unk in sample #DIV/0!

 AxCsVs/
Note Cx= (Ax+s)VX
Volume Standard A660
0.00 0.209 Absorbance vs. Volume
5.00 0.329 0.9
10.00 0.455
0.8 f(x) = 0.0250285714x + 0.2061428571
15.00 0.581 R² = 0.9999374531
20.00 0.707 0.7

25.00 0.833 0.6

0.5

0.4

Concentration Std 1.00E-05 0.3

C Pd 1.00E+01 0.2

0.1
Slope 0.025028571
Intercept 0.206142857 0
0.00 5.00 10.00 15.00

Conc of Pd 8.24E-06
orbance vs. Volume Standard

x + 0.2061428571

10.00 15.00 20.00 25.00 30.00

Known parameters E(365) Co E(700) Co E(365) Ni
3529 428.9 3228

Data A(365) for mixture A(700) for mixture

0.426 0.026

Solution Iteration
Conc Co 6.745112730993E-05
Conc Ni 5.901608417216E-05 5.901608417216E-05

Predicted A (365)
Predicted A (700)
E(700) Ni
10.2
 MOLE RATIO

Conc Ligand,M Conc Metal, M Conc L/ Conc M Abs

0.6
1.00E-05 3.70E-05 0.2702702703 0.131
2.00E-05 3.70E-05 0.5405405405 0.265
0.5
3.00E-05 3.70E-05 0.8108108108 0.396
4.00E-05 3.70E-05 1.0810810811 0.468 0.4
5.00E-05 3.70E-05 1.3513513514 0.487
6.00E-05 3.70E-05 1.6216216216 0.498 0.3
8.00E-05 3.70E-05 2.1621621622 0.499
0.2
1.00E-04 3.70E-05 2.7027027027 0.5
(above not needed)
0.1

0
0 0

Absorbance vs. M
START HERE

Absorbance
0.6
Conc. Ligand Absorbance
1.00E-05 0.131 0.5
2.00E-05 0.265 f(x) = 50x + 0.495
R² = 1
3.00E-05 0.396 0.4
4.00E-05 0.468 f(x) = 13250x - 0.001
R² = 0.999957282
5.00E-05 0.487 0.3
6.00E-05 0.498
8.00E-05 0.499 0.2

1.00E-04 0.5
0.1

0
0.00E+00 2.00E-05 4.00E-05 6.00E-0
Concentratio

Slope 1 13250
Intercept 1 -0.001
Slope 2 50
Intercept 2 0.495

(conc of ligand) Intersection 3.757576E-05 Intersection Abs 0.498879

Intersection Abs 0.496879
conc metal 3.70E-05 rounded 0.5
conc Ligand 3.757576E-05
 ratio 1.015561016

Molar Absorptivity 13223.3871 Molar Absorptivity (rounded) 13306.45

Note: (L/molcm)

In mole ratio
conc one reactant constant
conc other reactant varied

1. find mole ratio

2. plot A vs. mole ratio (or plot A vs. conc sa varying)
3. obtain two straight lines
4. intersection is mole ratio of complex
5. kuhaon ang ratio
 Plot of All Points
0.6

0.5

0.4

0.3

0.2

0.1

0
0 0.5 1 1.5 2 2.5 3

Absorbance vs. Mole Ratio Absorbance vs. Mole Ratio

Absorbance

0.6

0.5
f(x) = 50x + 0.495 f(x) = 50x + 0.495
R² = 1 R² = 1
0.4
(x) = 13250x - 0.001 f(x) = 13250x - 0.001
² = 0.999957282 R² = 0.999957282
0.3

0.2

0.1

0
2.00E-05 4.00E-05 6.00E-05 8.00E-05 1.00E-04 1.20E-04 0.00E+00 2.00E-05 4.00E-05 6.00E-05 8.00E-05 1.00E-04
Concentration of L, M Concentration of L, M
vs. Mole Ratio

6.00E-05 8.00E-05 1.00E-04 1.20E-04

entration of L, M
 SLOPE RATIO
1. Plot Abs vs. Conc Metal
Input Data of First (conc L, plot M)
Conc CDA, M 1.00E-03
Absorbance vs. Concentration
C Ni M Absorbance 0.8
5.00E-06 0.051 0.7
f(x) = 10277.11781889x - 0.000381889
1.20E-05 0.123 R² = 0.9999987806
0.6
3.50E-05 0.359
0.5
5.00E-05 0.514

Absorbance
6.00E-05 0.616 0.4
7.00E-05 0.719 0.3

0.2

0.1

0
0.00E+00 1.00E-05 2.00E-05 3.00E-05 4.00E-05 5.00E-05 6.00E-0
SLOPE (Eb/x) 10277.11782 Concentratio of Nickel, M

LINEST VALUES

10277.11781889 -0.000381889 slope int

5.6743144243 0.000257895 SD slope SD int
0.9999987806 0.000332 r2 sd y
3280317.4664313 4 F df
0.3615695591 4.408958E-07 ss reg ss resid

2. Plot Abs vs. Conc Ligand

Input Data of Second (conc M, plot Ligand) Absorbance vs. Ligand Concentrati
Conc of Ni, M 1.00E-03 0.3

0.25
C CDA M Absorbance f(x) = 3426.5679781847x - 0.0002119205
9.00E-06 0.031 0.2 R² = 0.9999840915
Absorbance

1.50E-05 0.051 0.15

2.70E-05 0.092
4.00E-05 0.137 0.1

6.00E-05 0.205 0.05

7.00E-05 0.24
0
0.00E+00 1.00E-05 2.00E-05 3.00E-05 4.00E-05 5.00E-05 6.00
Concentration of Ligand, M

SLOPE (Eb/y) 3426.567978

Slope Ratio (y/x) 2.999245275 y 3

 x 1

Molar Abs 10277.11782

Molar Abs 1 10277.11782

Molar Abs 2 10279.70393

Molar Abs Ave 10278.41088

in slope ratio

assumption
1. in excess ang isa ka reactant to complete rxn
2. Beer's Law followed
3. complex absorbs at wav chosen

steps
1. Plot Abs vs conc of the two line 1 Mx
2. get two eqns of line line 2 Ly
3. get that MxLy
slope of line 1 Eb/x
slope of line 2 Eb/y

4. get ratio
bs vs. Conc Metal

nce vs. Concentration Absorbance vs. Concentration

0.8

0.7
81889x - 0.000381889 f(x) = 10277.11781889x - 0.000381889
06 R² = 0.9999987806
0.6

0.5

Absorbance
0.4

0.3

0.2

0.1

0
3.00E-05 4.00E-05 5.00E-05 6.00E-05 7.00E-05 8.00E-05 0.00E+00 1.00E-05 2.00E-05 3.00E-05 4.00E-05 5.00E-05 6.00E-05 7.00E-05 8.00E-05

Concentratio of Nickel, M Concentratio of Nickel, M

ce vs. Ligand Concentration Absorbance vs. Ligand Concentration

0.3

0.25
f(x) = 3426.5679781847x - 0.0002119205
6.5679781847x - 0.0002119205 0.2 R² = 0.9999840915
9840915
Absorbance

0.15

0.1

0.05

0
0.00E+00 1.00E-05 2.00E-05 3.00E-05 4.00E-05 5.00E-05 6.00E-05 7.00E-05 8.00E-0
05 3.00E-05 4.00E-05 5.00E-05 6.00E-05 7.00E-05 8.00E-05
Concentration of Ligand, M Concentration of Ligand, M

Ratio= sl 1/sl2
 ation

-05 6.00E-05 7.00E-05 8.00E-05

M

Concentration

0002119205

05 5.00E-05 6.00E-05 7.00E-05 8.00E-05

of Ligand, M
 CONTINUOUS VARIATION
CM 1.25E-04
CL 1.25E-04

0.800
VL VM VM+VL VL/(VM+VL) Absorbance f(x) = 1.7x + 0.005
R² = 0.998339095
0 10 10 0 0.000 0.700
1 9 10 0.1 0.174
0.600
2 8 10 0.2 0.353
3 7 10 0.3 0.530 0.500

Absorbance
4 6 10 0.4 0.672
5 5 10 0.5 0.723 0.400

6 4 10 0.6 0.673
0.300
7 3 10 0.7 0.537
8 2 10 0.8 0.358 0.200
9 1 10 0.9 0.180
0.100
10 0 10 1 0.000
0.000
0 0.2

Slope 1 1.7
Intercept 1 0.0058
Slope 2 -1.8
Intercept 2 1.8
Volume Ratio 0.51262857 x
y

in continuous variation

1. metal and ligand kay same ang concentration

2. total volume and total moles is constant
3. mole ratio varies eg. 9:1, 8:2

steps
1. get corrected absorbance
2. plot Absorbance versus (VM/(VM+VL) Vm= volume metal Vl= volume ligand
Optional

plot Absorbance versus (VM/(VM+VL)

plot Absorbance versus (VL/(VM+VL)

then divide 1/2- ratio

 Absorbance vs. VL/(VL+VM) 0.800 Ab
0.800 f(x) = 1.7x + 0.0058
f(x) = 1.7x + 0.0058 f(x) = - 1.789x + 1.7894 R² = 0.9983390954
R² = 0.9983390954 R² = 0.9999956257 0.700
0.700

0.600
0.600

0.500
0.500

Absorbance
Absorbance

0.400
0.400

0.300
0.300

0.200
0.200

0.100
0.100

0.000
0.000 0 0.2
0 0.2 0.4 0.6 0.8 1 1.2
(VL/ (VM +VL), mL
 0.800 Absorbance vs. VL/(VL+VM)
f(x) = 1.7x + 0.0058 f(x) = - 1.789x + 1.7894
R² = 0.9983390954 R² = 0.9999956257
0.700

0.600

0.500
Absorbance

0.400

0.300

0.200

0.100

0.000
0 0.2 0.4 0.6 0.8 1 1.2
(VL/ (VM +VL), mL
 CONTINUOUS VARIATION
CM 1.25E-04
CL 1.25E-04

VL VM VM+VL VM/(VM+VL) Absorbance Absorba

0 10 10 1 0.000 0.800
1 9 10 0.9 0.174 f(x) = 1.789x + 0.0004
R² = 0.9999956257
2 8 10 0.8 0.353 0.700
3 7 10 0.7 0.530
0.600
4 6 10 0.6 0.672
5 5 10 0.5 0.723 0.500

Absorbance
6 4 10 0.4 0.673
0.400
7 3 10 0.3 0.537
8 2 10 0.2 0.358 0.300
9 1 10 0.1 0.180
10 0 10 0 0.000 0.200

0.100

Slope 1 -1.769 0.000

0 0.2
Intercept 1 1.7679
Slope 2 1.789
Intercept 2 0.0004
Volume Ratio 0.49676785 x
y

molar Abs Abs intersection 0.889118

0.889118
sol1 1392 1392
sol2 2824 1412 Kf complex 0.000626
sol3 4240 1413.333
sol8 2864 1432
sol9 1440 1440
sol7 4296 1432

molar E Abs 1420.222

in continuous variation

1. metal and ligand kay same ang concentration

2. total volume and total moles is constant
3. mole ratio varies eg. 9:1, 8:2

steps
1. get corrected absorbance
2. plot Absorbance versus (VM/(VM+VL) Vm= volume metal Vl= volume ligand

Optional

plot Absorbance versus (VM/(VM+VL)

plot Absorbance versus (VL/(VM+VL)

then divide 1/2- ratio

 Absorbance vs. Vm/(Vm+VL)
0.800
f(x) = 1.789x + 0.0004 f(x) = - 1.769x + 1.7679
R² = 0.9999956257 R² = 0.9999699628
0.700

0.600

0.500

0.400

0.300

0.200

0.100

0.000
0 0.2 0.4 VM/ (VM+VL)
0.6 0.8 1 1.2

Absorbance vs. Vm/(Vm+VL)

0.800
f(x) = 1.789x + 0.0004 f(x) = - 1.769x + 1.7679
R² = 0.9999956257 R² = 0.9999699628
0.700

0.600

0.500
Absorbance

0.400

0.300

0.200

0.100

0.000
0 0.2 0.4 VM/ (VM+VL)
0.6 0.8 1 1.2
CM 1.00E-04 1.2
CL 1.00E-04 f(x) = 1.968x - 0.0034
R² = 0.9998079389
1

VL VM VM+VL VL/(VM+VL) ABS

0.8
0 10 10 0 0.002
1 9 10 0.1 0.188
0.6
2 8 10 0.2 0.387
3 7 10 0.3 0.588
4 6 10 0.4 0.786 0.4

5 5 10 0.5 0.931
6 4 10 0.6 0.993 0.2
7 3 10 0.7 1.059
8 2 10 0.8 0.972 0
0 0.2 0.4
9 1 10 0.9 0.588
10 0 10 1 0.001

Slope 1 1.968
Intercept 1 -0.0034
Slope 2 -5.87
Intercept 2 5.871
Volume Ratio 0.74947691
1.2
f(x) = 1.968x - 0.0034 f(x) = - 5.87x + 5.871
R² = 0.9998079389 R² = 1
1

0.8

0.6

0.4

0.2

0
0 0.2 0.4 0.6 0.8 1 1.2
F Fo Conc Fo/F
180 180 0.000 1.0000 Chart Title
180 87.5 0.005 2.0571
14.0000
180 58 0.010 3.1034
180 43.2 0.015 4.1667 12.0000
180 35 0.020 5.1429 f(x) = 209.9461725408x + 1.0091546076
10.0000 R² = 0.9998800963
180 28.5 0.025 6.3158
180 24.5 0.030 7.3469 8.0000
180 19.1 0.040 9.4241
6.0000
180 15.7 0.050 11.4650
4.0000

2.0000

0.0000
0.000 0.010 0.020 0.030 0.040

slope 209.9462 KSV

T 1
Kq 209.9462
LINEST
209.9462 1.009155 #N/A
0.868963 0.023127 #N/A
0.99988 0.040292 #N/A NOTE:
58373.19 7 #N/A kq less than or equal to10^10
94.7664 0.011364 #N/A greater than 10^10
#N/A #N/A #N/A

1+kqT=
1+Ksv[Q]= Fo/F
 Chart Title

725408x + 1.0091546076
963

0.020 0.030 0.040 0.050 0.060

dynamic
static
Known parameters E(480) Pd E(635) Pd E(480) Au
3.55E+03 5.64E+02 2.96E+03

Data A(480) for mixture A(635) for mixture

0.533 0.59

Solution Iteration
Conc Pd 3.601782612222E-05
Conc Au 0.0001201091 0.0001201091

Predicted A (365)
Predicted A (700)
E(635) Au
1.45E+04
Volume Standard A660
0.00 0.24 Absorbance vs. Volu
5.00 0.437 1.2
10.00 0.621
15.00 0.809 1
f(x) = 0.0382x + 0.2412
20.00 1.009 R² = 0.999805964
0.8

0.6

Concentration Std 1.11E+01 0.4

Volume of Aliquot Sample 1.00E+01
0.2
Slope 0.0382
Intercept 0.2412 0
0.00 5.00 10.00

Conc of Analyte 7.01E+02

Cx= bCs/mVx

if naa kay aliquots with different volumes nga imong gibutang sa volume total
dayon gibutangan nimong lain2 nga volume sa standard with known concentration
 Absorbance vs. Volume Standard

0382x + 0.2412
99805964

5.00 10.00 15.00 20.00 25.00

Single Standard

Cx= A1CsVs/ (A2Vt-A1Vx)

Cs 0.05
Vs 1
Vx 25

A1 0.428
A2 0.517

Vt 26

Cx 0.007805

dilution factor