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I.

TITLE: CALIBRATION OF VOLUMETRIC GLASSWARE

II. INTRODUCTION

Burettes, pipettes and volumetric flask are used for accurate quantitative measurements. When
using apparatus of standard quality, it is not desirable to attempt calibration of such apparatus for three
reasons. Firstly, the process of calibration is time consuming. Secondly, due to possibility of introduction
of errors in calibrating the apparatus, the apparatus thus calibrated by an inexperienced beginner
maybe less accurate that the apparatus calibrated by the manufacturer. Thirdly, the error permitted in a
determination may take care of any inaccurate in the calibration of apparatus. However, if the
apparatus is being use is not of standard quality, it is advisable to check the calibration of apparatus
before performing any measurements with such an apparatus. In this experiment, you will perform the
calibration of pipette, a burette and a volumetric flask.

Volumetric flask (measuring flask or graduated flask) is a piece of laboratory apparatus, a type of
laboratory flask, calibrated to contain a precise volume at a certain temperature. Volumetric flasks are
used for précised dilution and preparation of standard solutions. These flasks are usually pear-shaped,
with a flat bottom, and made of glass and plastic. The flasks mouth is either furnish with a plastic
snap/screw cap or fitted with a joint to accommodate a PTFE or a glass stopper. The neck of volumetric
flask is elongated and narrow with an etched ring graduation marking. The marking indicates the volume
of liquid contained when filled up to a point. The marking typically calibrated “to contain” (marked “TC”
or “IN”) at 20°C indicated correspondingly on a label. The flask’s label also indicates the nominal volume,
tolerance, precision class, relevant manufacturing standard and the manufacturer’s logo. Volumetric
flasks are of various sizes, containing from 1 millimeter to 20 liters of liquid.

A burette is a graduated glass tube with a tap at one end, for delivering known volumes of a liquid,
especially in titrations. It is a long, graduated glass tube, with stopcock at its lower end a tapered
capillary tube to the burette tip is controlled by the stopcock valve. There are two main types of burette,
the volumetric burette and the piston burette or digital burette. A volumetric burette delivered
measured volumes of liquid. Piston burettes are similar to syringes, but with a precision bore and a
plunger. Piston burettes may be manually operated or may be motorized. A weight burette delivers
measured weights of liquid.

A pipette is a laboratory tool commonly used in chemistry, biology and medicine to transport a
measured volume of liquid, often as a media dispenser. Pipettes come in several designs for various for
various purposes with differing levels of accuracy and precision, from single piece glass pipettes to more
complex adjustable or electronic pipettes. Many pipettes work by creating a partial vacuum above the
liquid-holding chamber and selectively releasing this vacuum to draw up and dispense liquid.
Measurement accuracy varies greatly depending on the style of laboratory pipette being used.
III. OBJECTIVE

This experiment is designed to introduce you to proper analytical laboratory techniques

and to the methods for evaluating analytical data.

IV. MATERIALS

 125-ml Volumetric flask

 25-ml Volumetric flask

 25-ml Buret

V. PROCEDURE

CALIBRATION OF VOLUMETRIC FLASK

 Place the empty volumetric flask on the balance and write down the weight of the

flask.

 Remove the flask from the balance.

 Then, carefully add distilled water just until the bottom of the meniscus rest on

the top of the mark on the neck of the flask.

 Measure the temperature of the water used.

 Reweigh

 Discard the waters and refill the flask.

 Repeat two more times.

 Calculate the true volume of the flask using the buoyancy correction factor.

 Calculate the mean and the correction factor.


CALIBRATION OF A PIPET

 Fill the pipet with room temperature distilled water. (record the

temperature of the water).

 Deliver the water to the weighing bottle as directed above.

 Reweigh the bottle. Taking care not to touch the bottle with your fingers.

 Repeat this procedure a minimum of 2 times.

 Record the temperature in the laboratory.

CALIBRATION OF BURET

 Record the temperature in the laboratory.

 Wash the buret as you did your pipet and allow it to drain.

 Close the stop cock.

 Hold the buret in your hand and using a transfer pipet small amount to

rinse your buret with the solution you are going to use to fill it.

 Drain completely into a waste beaker.

 Properly place the buret into the buret clamp.

 Use a funnel and refill the buret with your titrant.

 Open the stopcock and drain the buret to the 0.00 ml mark.

 Be sure that there are no air bubbles in the buret or in the stopcock tip.

 Touch the beaker to the tip of the buret to remove the hanging drop.

 Allow the buret to stand for 5 minutes.

 Weigh the mass of the empty weighing bottle.

 Record this as the initial reading of the volume in the buret.


 Place the weighing bottle under the buret.

 Open the stopcock allow 5ml of water to drain into the bottle. Close

stopcock.

 Wait 30 second for the film of liquid on the walls of the buret to drain.

 Read the buret to the nearest 0.011.ml.

 Weight the weighing bottle to the nearest to 0.1mg. Subtract original

weight to new weight.

 Repeat the procedure in 5ml increments to 25ml. Same weighing bottle.

Delivered 25ml from buret at this point.

 Refill the buret and repeat 2 more times.

 Record the temperature in the laboratory.


V1. DATA GATHERED

 PIPET

Calibration of pipet 10 ml
Mean ( Correction mL) 7.8915 ml
Mean True Value 6.9024 ml

 BURET

TRIAL # 1 TRIAL # 2 TRIAL # 3


Mass of the bottle (g) 99.6803 99.6803 99.6803
Mass of the bottle plus water 126.9708 126.9998 126.9353
Mass of water (g) 27.2905 27.3195 27.255
Final Reading buret (ml) 27.6155 27.8123 27.5624
Initial reading buret (ml) 0.5 10.01 20.45
Volume delivered buret (ml) 27.1155 34.8023 24.4124
Total volume delivered, buret (ml) 44.1155 34.8023 24.4124
True volume calculated from mass of water (ml) 27.3725 27.4932 27.423
Correction (ml) -0,082 -0.3191 -0.1394

 VOLUMETRIC FLASK

VII. DISCUSSION

VIII. CONCLUSION

VIIII. DOCUMENTATION

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