PRODUCT BROCHURE Real-Time PCR Systems

Applied Biosystems 7500 Fast, 7500 and

7300 Real-Time PCR Systems
Real Fast. Real Versatile. Real Value. Real choices from the leader in real-time PCR.
The latest innovations in
Real-Time PCR, built on a
solid reputation for excellence

Since we pioneered real-time PCR over a decade ago, Applied Biosystems has continued
to develop the technology to provide more powerful solutions to labs of all sizes.

Applied Biosystems offers a family of Real-Time PCR instruments with the 96-well block
format. The Applied Biosystems 7500 Fast Real-Time PCR System represents the latest
innovation in real-time technology—Fast thermal cycling, delivering high-quality results in
as little as 30 minutes.

The Applied Biosystems family of real-time solutions combines innovative thermal cycling
systems, powerful software, optimized reagents, your choice of off-the-shelf or custom
assays, and superior support for a variety of applications. And, of course, all systems are
backed by Applied Biosystems’ unmatched track record of quality and long-term reliability.

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Real Fast
The Applied Biosystems 7500 Fast Real-Time PCR System

The Applied Biosystems 7500 Fast Real-Time PCR System

offers maximum performance in the minimum time for labs
running a variety of applications, including High Resolution
Melting (HRM) analysis.

Results in 30 minutes
Fully optimized for Fast cycling, the 7500 Fast System delivers
high-quality results in as little as 30 minutes. The 7500 Fast
System enables more runs per day with the excellent
performance equivalent to that offered by the 7500 System.

The Applied Biosystems 7500 Fast System is the original Fast

solution including validated Fast reagents and over 700,000
TaqMan® Gene Expression Assays.

Built for speed

• Specially designed Fast block ensures thermal uniformity
at top speeds Amplification plot from a 7500 Fast system above shows results from a TaqMan
Fast RNAse P Instrument Verification Plate. Using this assay, the 7500 Fast
• TaqMan Fast Universal PCR Master Mix and Fast SYBR
® ®
platform can distinguish between samples containing 5,000 copies and 10,000
Green Master Mix enable-rapid results with excellent assay DNA template copies respectively, with a statistical confidence level of 99.7%
and a run time of about 30 minutes.
performance equivalent to that of standard real-time PCR
• Fast optical plates ensure excellent precision in 5–30 μL
reaction volumes
• Fast ramp rates enable rapid results without compromising
extension times or assay quality

Supports many applications

Applications include gene expression analysis, absolute
quantitation, SNP genotyping and +/– assays utilizing internal
positive controls. The 7500 Fast Real-Time PCR System is
optimized for use with standard dye sets: FAM™/SYBR® Green I,
VIC®/JOE™, NED™/ TAMRA™/ Cy3™, ROX™/Texas Red®, and Cy5™
dyes. The 7500 Fast can also be used for high resolution melting
analysis using the new Applied Biosystems HRM Software.

To facilitate many of these applications, Applied Biosystems

provides preformulated, ready-to-use, quality-tested, TaqMan®
assays for use with the 7500 Fast System. Use these assays
to reduce your assay optimization effort and expense.
Difference plot generated by Applied Biosystems’ HRM Software. A 96 bp-long
fragment of the human Keratin 23 gene containing a G>A variant was amplified
from DNA samples and melted on the 7500 Fast System. Three replicates of each
genotype are shown, wild-type homozygote A/A (green), variant homozygote G/G
(red), and heterozygote G/A (blue).
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Real Versatile
The Applied Biosystems 7500 Real-Time PCR System

The Applied Biosystems 7500 Real-Time PCR System is a

powerful platform for labs requiring superior performance
and maximum dye versatility.

Built for performance

• Powerful, five-color platform is calibrated for the broadest
range of dyes available: FAM™/SYBR® Green I, VIC®/JOE™,
NED™/ TAMRA™/ Cy3™, ROX™/Texas Red®, and Cy5™ dyes
• Specialized optical system enables easy and accurate
calibration to accommodate new dyes without the addition
of new filter sets
• Advanced multicomponenting algorithm minimizes spectral
crosstalk – superior for multiplexing
• User-friendly software includes plate setup wizards, multi-
plate data viewing capabilities, and advanced analysis tools Complete validation solutions
to make data processing simple and straightforward Applied Biosystems offers IQ/OQ, a validation support
• Thermal cycling block is built into the body of the system solution. A certified Applied Biosystems Service Engineer will
to reduce environmental exposure and contamination risk assist you with your Installation Qualification and Operational
Qualification (IQ/OQ) or Instrument Performance Verification
The SDS v1.4 21 CFR Part 11 Module (IPV) process as part of your overall system validation.
The SDS v1.4 21 CFR Part 11 Module is available for the 7500
and 7500 Fast Systems. This module is a powerful tool for
assisting with 21 CFR Part 11 compliance while still offering
the flexibility of user customizable configuration settings.
• Individual user log-ins can be added for up to four user
groups, each group with designated permission settings
• User-customizable permission settings include fourteen
system activities, e-signature authority designation, and
additional security settings to give you maximum control
over your compliance efforts
• Audit trails can be enabled or disabled depending on your
traceability needs
• A selection of e-signatures is available to ease e-signatures
into your workflow
Easy-to-use 21 CFR Part 11 configuration settings allow the custom
configuration of user permissions and the ability to turn 21 CFR Part 11
features on and off.
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Real Value
The Applied Biosystems 7300 Real-Time PCR System

The Applied Biosystems 7300 Real-Time PCR System is an

economical solution setting the standard for labs requiring a
quality platform at an affordable price.

Proven performance and superior data quality

at an excellent value
The 7300 Real-Time PCR System balances exceptional quality
with budget sensitivity. Advanced multicolor detection
capabilities allow you to perform a wide variety of applications
with the highest confidence in your results.

Applications include gene expression analysis, absolute

quantitation, SNP genotyping, and +/– assays utilizing internal
positive controls. The 7300 System is optimized for use with
standard dye sets: FAM™/SYBR® Green I, VIC®/JOE™, NED™,
TAMRA™, and ROX™. Log of change in normalized reporter fluorescence plotted against PCR cycle
number. This plot from the 7300 System illustrates 9 logs of linear dynamic
Built for flexibility range for a TaqMan ® assay of plasmid DNA containing the hCCNB1 target
sequence in tenfold serial dilutions (hCCNB1 is human g2/mitotic specific
•Set up the plate before or after the completing the run cyclin b1, chromosome 5, 5q12).
• Correct plate setup mistakes after the run without losing
collected data
• One-click graphical export to PowerPoint or JPEG for
top-notch presentations
• Flexible system can be used as a plate reader or a regular
thermal cycler
• Real-time amplification monitoring and protocol options
enable extra PCR cycles to be added during a run
• Lamp-lifetime monitoring ensures highest possible
instrument performance

Results you can trust—every time

The Applied Biosystems 7300 System offers both proven
reliability and superior data quality—exactly what you would
expect from the leader in real-time PCR systems.
Multiplexed TaqMan ® assays on the 7300 System showing amplification of
cDNA (96 samples) using probes labeled with VIC ® (green) and FAM ™ (black)
reporters for the 18S and TGF-beta target sequences, respectively.

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Real Powerful
New 7500 Software v2.0

The Applied Biosystems 7500 Software v2.0 sets a new

standard for simplicity and usability. This software features
intuitive workflows that help you get expert results quickly
and easily.

7500 Software v2.0 Software Highlights

• Design Wizard to walk you through experimental design

• Advanced setup option offers flexibility for more complex

applications, such as multiplexing
• Quick-start setup option allows you to begin a run
immediately and enter plate information later
• Pipetting protocols and recipes help you set up
experiments quickly
• Troubleshooting flags to help you diagnose and resolve
problematic experiments
Plate setup wizard is streamlined for each application and walks you through
• Email notification when a run is complete unknowns, standards, and controls. Copy and paste sample names from Microsoft
Excel. Save templates for future use—never set up the same plate twice.
• One–click graphical export to PowerPoint and JPEG

New High Resolution Melting (HRM) Software

Use the Applied Biosystems High Resolution Melting (HRM)
Software to perform more sophisticated melting analysis
with an easy to follow workflow and minimal subjective data
analysis steps. The HRM Software is available on the 7500 Fast
Real-Time PCR System, which delivers precise results in
a standard 96-well format.
• Shortens analysis time by autocalling genotypes and
automatically omitting the no template controls
• Minimizes subjective analysis by automatically grouping
unknown variant clusters
• Allows easy data review with customizable multi-plot views,
expandable windows and one–click color assignment to
highlight curves of interest
• Facilitates data presentation with the option to export data The Applied Biosystems High Resolution Melting (HRM) Software is the
easiest to use melting analysis software enabling real-time PCR melt curve
and graphs directly to PowerPoint or as JPEG files
assays to be used more accurately for mutation scanning and genotyping.
• No temperature shift required — distinguish between
homozygous mutants and wild type more easily

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More Powerful Gene Expression Study Package
• Import an unlimited number of Comparative Ct
(Relative Quantitation) plates to one study
• View data by biological replicate group or technical
replicate group
• Normalize to multiple endogenous controls
• Enter known efficiencies to adjust RQ values for each target
• View amplification plot, multicomponent plot and QC summary
within the study to easily identify and eliminate outliers
• Preview the effect of modified analysis settings before
permanently applying them to results
Gene Expression Study Package allows simultaneous analysis of unlimited
96-well plates of data without restricting viewing to dye-layer tabs. You can
specify the color and order of gene targets, then export the graph directly to
PowerPoint.
With the unknowns plotted on the standard curve, removal of outliers is simple
and straightforward.
Expanded Melt Curve Options
• Option to use either a continuous melt for standard post
PCR melt curves, or step and hold melt curve for melting
experiments that require more resolution and accuracy
• More flexible melt curve protocols allow you to customize
the ramp rate, temperature span and data acquisition
• Identify the Tm of up to three melt curve products per well
• Melt curve data can be imported into the Applied
Biosystems High Resolution Melting (HRM) Software for in
depth analysis
Flexible melt curve options enable a variety of melting curve based
applications, including HRM.
A SNP autocaller automatically determines genotypes, and generates an
intuitive graphic representation of results in a cluster plot report that helps
you view data across populations or samples.
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Real Convenient
The whole genome at your service and ready to use
Applied Biosystems TaqMan® Genomic Assays are moving
genetic discovery to the next level. Both public and Celera
genomic information is used to design optimal primer and probe
sets. Based on our 5’ nuclease chemistry, these assays are
ideal for both quantitative gene expression and SNP genotyping
applications. All TaqMan Genomic Assays are provided in a
single-tube format and designed to run using universal cycling
conditions that require no additional optimization.
Our current offering of more than 700,000 TaqMan® Gene
Expression Assays is a comprehensive collection of pre-designed
primer and probe sets that enable researchers to quickly and
easily perform quantitative gene expression studies on human,
mouse, rat, monkey, dog, Arabidopsis, and Drosophila genes.
Additionally, our collection of over 4.5 million TaqMan® SNP
Genotyping Assays for human and mouse provide a flexible
technology for detecting must-have SNPs.
To complement our offering of off-the-shelf products, our
custom TaqMan® assays are the best solution for customer-
specific gene expression or genotyping assays. Simply submit
your sequence, using a secure and confidential ordering
mechanism, and we will deliver a single-tube format of either
your gene expression or genotyping assay for any species.
TaqMan® Genomic Assays
You can search for TaqMan® Genomic Assays by gene symbol,
public accession numbers, molecular function, or biological
process. You can find more about TaqMan Gene Expression
Assays at www.allgenes.com and more about TaqMan SNP
Genotyping Assays at www.allsnps.com. You’ll also find useful
data and valuable links to relevant references at both sites.
Now you can take your real-time PCR as far as you need to go
To learn more about the new Applied Biosystems 7500 Fast,
7500 or 7300 Fast Real-Time PCR Systems, call 1.650.638.5800,
or visit us on the Web at www.appliedbiosystems.com

ORDERING INFORMATION
Description Part Number
7300 Real-Time PCR System with Notebook Computer 4351101
7300 Real-Time PCR System with Tower Computer 4351103
7500 Real-Time PCR System with Notebook Computer and SDS Software 4351104
7500 Real-Time PCR System with Tower Computer and SDS Software 4351105
7500 Fast Real-Time PCR System with Notebook Computer and SDS Software 4351106
7500 Fast Real-Time PCR System with Tower Computer and SDS Software 4351107
7500 Fast System Upgrade Kit 4362143
7500 System SDS v1.4 Software 21 CFR Part 11 Module 4377354
7500 Fast System SDS v1.4 Software 21 CFR Part 11 Module 4377355
Notebook Computer for 7300/7500/7500 Fast System 4359286
Tower Computer for 7300/7500/7500 Fast System 4359284
17” Flat-Panel Monitor 4346944

For Research Use Only. Not for use in diagnostic procedures.

NOTICE TO PURCHASER
The Applied Biosystems 7300, 7500 and 7500 Fast Real-Time PCR Systems are real-time thermal cyclers covered by one or more of US Patents Nos. 7,504,241, 5,475,610,
5,602,756, 6,703,236, 6,814,934, 6,677,151, 7,295,316, 7,289,217, 7,238,517, 6,982,166, 7,008,789, 7,183,103 and corresponding claims in their non-US counterparts, owned by
Life Technologies Corporation. No right is conveyed expressly, by implication or by estoppel under any other patent claim, such as claims to apparatus, reagents, kits, or methods
such as 5’ nuclease methods. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive,
Foster City, California 94404, USA.
© 2009 Life Technologies Corporation. All rights reserved. Trademarks of Life Technologies Corporation and its affiliated companies: Applied Biosystems®, AB (Design)®, FAM™,
JOE™, NED™, ROX™, SYBR®, TAMRA™, VIC®. TaqMan is a registered trademark of Roche Molecular Systems, Inc. All other trademarks are the sole property of their respective
owners.
Printed in the USA. 04/2009 Publication 117BR05-09

Headquarters International Sales

850 Lincoln Centre Drive | Foster City, CA 94404 USA For our office locations please call the division
Phone 650.638.5800 | Toll Free 800.345.5224 headquarters or refer to our Web site at
www.appliedbiosystems.com www.appliedbiosystems.com/about/offices.cfm