Experiment 2: Prelab Worksheet

(Hand in at the start of Experiment 2)

Name: Date: Jii_ Section: Grade:

All information needed to complete this worksheet can be found in the pre-lab information and
calculations sections of the lab manual. Read this introductory material first!
• Record all values with the correct number ofsiqnificant figures and units.
• Place all answers on the linewhen provided.
• Show calculations for any numerical answers; work must be shown to receive credit.
• See any 102 TA in the help office before your prelab is due if you have any questions.
• Each question isworth 2 points.

1. What is the purpose of chromatography?

i:° 4e Use-I as <^ [¢,Ae, c.i;e.1„t.r.,;,.a_ #a Hc=+<f n`.* tLL Sp;<;;;` MurfuJ±T c;~cJ +¥f ro> .-I C+e'`'-`` 1®1> f](Qqu+ t+ a":a

2. Draw a chromatogram in the box with a spot located at an Rf = 0.25. Use the numbers to
the left of the chromatogram to estimate your values.

Sowenf Fror`+ l`'nlL

3. Draw lines to represent the solvent start and
the solvent front. Label both lines.

4. Show the calculation of this Rf value.

0``,+£Qre. rtye, a.o`<s Crvuro ~ >7&f I:v^L 7D <fcJ+ cv.rbrS

EE=I
D;S/`rlceSc/ucvd~evr.3C:yur^ S+af i I,(^rL -i> So/V Q~+frtoJ IJ`

>is/a.,tc sJveho| r^Ovcs = ae r^rft

S po+ a,S+an`a f+on
ch = lonG|
® oiv a,r,+ a tc`. + a `.vA.
i;;+C7fa 54of /,-®v4> =-Z.I p-M

`n:+,'4' S4ot
•.`...:.,.`.?.... - Zl r`-
= C,. ZS Sc>IV®r.t a+c`r+ I{N,

$ 4- ys-qu

5. What do you call the chromatography paper after the experiment is completed?

Ams;Nor.. _Cl r a n a fo c( On

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6. Identify the 2 mobile phases used in the experiment.
Mobile Phase 1: „¢ /c ,

Mobile phase2: S~ Ajca cI Sc3>/cait<-oh

7. Identify the stationary phase in the lab. Ariswer.. CJqro ipr`io

8. a.) Can you still use your data if the mobile phase in the beaker covers the spot of dye you
are trying to separate? b.) What should you do if this happens?

b.Lr2ZiJ oV~ 7fr`~ +L bi7tL~ha a`J fjr/" A.~ n.+ri.{;I .nJ frjvf.L r.W` r`4>. .j b.L-sA#.ro

9. Which is less soluble in the mobile phase, a spot at the top of the chromatogram or a spot
at the bottom?

Answer: o+ a.+ ZLf< bej+Dv< a/ (I.r®-.J=-3

10.Where do you dispose of the mobile phases in this lab?

AlrvsINe:I.r>;i/-}k Df a,.j!^ fno±;/a all.esj.s ior.fat /:i,vr4 wee/1 <a^t2;v,e; ,a (4bo

I._'

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