Name: _______________ Date: __________

1. (a) What is a tissue?

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(b) Name a tissue found in plants

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(c) Give a function of the tissue referred to in part (b)

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(d) Name a tissue found in animals

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(e) Give a function of the tissue referred to in part (d)

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(f) Give one way in which the tissue referred to in (ii) is adapted to carry out its function(s).

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(f) Explain what is meant by the term tissue culture

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(g) Give two examples of the use of tissue culture

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2. Organs are found in both plants and animals. What is meant by the term
organ? Name one organ found in plants.
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3. a) State a function of the cell membrane
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(b) State one feature that would allow you to identify a eukaryotic cell
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4. The diagram shows the structure of Amoeba.

a) Is the cell of Amoeba prokaryotic or eukaryotic?

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b) Give a reason for your answer to part (a)
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5. Select the correct cell component from the following list and write it opposite
its partner in column B.
ribosome, vacuole, chloroplast, cell membrane, mitochondrion
Column A Column B
Contains chlorophyll
 Site of protein formation
Site of energy release
Site of storage of water, salts and sugars

Allows osmosis to occur

6. A microscope has an eyepiece lens marked ×10 and an objective lens marked
×20. What is the total magnification of the image?
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7. What is a selectively permeable (semi-permeable) membrane?
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8. In one of your laboratory activities you isolated DNA from a plant tissue.
Where in plant cells is DNA found?
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9. In school, a light microscope is normally used to examine cells and tissues.
Name a more powerful type of microscope that is used to show what cells are
made of in much greater detail (cell ultrastructure).
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10. For what purpose did you use each of the following in the course of your
practical activities?
i) Cover slip.
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ii) Methylene blue.
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9. (a) Name the parts of the light microscope labelled A and B.
___________

___________

(b) Answer the following questions in relation to obtaining and staining a sample
of plant cells and viewing them under the microscope.
(i) From what plant did you obtain the cells? __________________
(ii) How did you obtain a thin piece of a sample of the cells and prepare it for
examination? _______________________________________________________
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(iii) What stain did you use on the cells?
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(iv) Describe how you applied the stain.
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(v) The objective lenses on a microscope are usually labelled 40X, 10X, and 4X.
Which objective lens should you begin with when using the microscope?
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(vi) Give one cell structure that you observed that indicated that the cells were
plant cells.
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10. The cells of an organism are described as eukaryotic.
Give two characteristic features of eukaryotic cells.
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11. Give an example of a catabolic reaction

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12. The graph shows how the rate of reaction of a carbohydrate-digesting enzyme
in the human alimentary canal varies with pH.

(a) A is said to be the enzyme’s ________________pH

(b) Suggest a temperature at which human enzymes work best ________

(c) What term best describes the shape of an enzyme?

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13. To which group of molecules do enzymes belong? ______________________

What is a denatured enzyme? ______________________________________________

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14. Answer the following by reference to an experiment that you carried out
to investigate the effect of pH on the rate of enzyme action.
a) Name the enzyme and substrate that you used.
b) Draw a labelled diagram of the apparatus that you used.
c) Explain how you varied the pH.
d) State one factor that you kept constant in this experiment.
e) How did you ensure that this factor was kept constant?
f) Label the axes and draw a graph of the results that you obtained.
15. a) Immobilised enzymes are sometimes used in bioreactors.

(i) What is a bioreactor?

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(ii) State one advantage of using an immobilised enzyme in a bioreactor.

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b) Answer the following questions in relation to an experiment that you carried

out to immobilize an enzyme and use that immobilised enzyme.

(i) Name the enzyme that you used _________________

Draw a labelled diagram of the apparatus that you used to immobilise the
enzyme.

(ii) Describe how you used this apparatus to immobilise the enzyme. In
your answer name the solutions that you used and explain their
purpose.

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___________________________________________________________________
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(iii) Describe briefly how you used the immobilised enzyme.

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(v) Describe how you carried out the investigation. In your answer you must refer
to the way that you measured the enzyme’s activity

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(vi) State the results that you obtained.

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16. Name four factors that influence the activity of an enzyme.

__________________
_______
_____________ _________________
_____________ _________________
17. As part of your practical activities you investigated the effect of temperature
on the rate of activity of an enzyme.
(i) Name the enzyme that you used
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(ii) Name the substrate with which the enzyme reacts
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(iii) How did you vary the temperature?
___________________________________________________________________
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(iv) How did you keep a constant pH during the investigation?
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(v) How did you measure the rate of activity of the enzyme?
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(vi) What was the result of your investigation?
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Indicate whether each of the following statements is true (T) or false (F) by
drawing a circle around T or F.

Example: The pulmonary artery carries blood to the lungs T F

Immobilised enzymes can act as catalysts T F

A nucleus is absent from human red blood cells. T F

If the eyepiece lens of a microscope is marked X10 and the objective lens is
marked X4, the total magnification is X14 T F

Plant cells have chloroplasts, animal cells do not have chloroplasts T F

Chloroplasts contain DNA. T F

The microscope lenses closest to the stage are the eyepiece lenses. T F

Plant cell walls are fully permeable. T F

Animal cells do not have membranes. T F

Cell membranes let only some molecules pass through T F

Human chromosomes are found in the nucleus T F

Bonus Question (Optional)

4. The following graph shows how the rate of photosynthesis varied when a plant was
subjected to varying levels of light intensity or carbon dioxide concentration.

(a) What is happening at A? _____________________________________________

(b) What is happening at B? _____________________________________________

(c) Suggest a reason for your answer in (b)

_________________________________________________________________________
_________________________________________________________________________

(d) Where in a cell does photosynthesis take place? ___________________________

(e) Give two sources of the carbon dioxide that is found in the atmosphere.

______________________________________________________________________________
______________________________________________________________________________

(f) Suggest one way in which the rate of photosynthesis of plants in a greenhouse
could be increased

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