IV.

RESULTS & DISCUSSION

RESULTS

TESTS OBSERVATIONS RESULTS

A1. Separated in half; cloudy white in Positive

upper part and indigo underneath

A. EFFECT OF TEMPERATURE A2. The color of the sample turned Positive

purple
A3: Very dark in color. Color is blue to Positive
dark blue.

B1. Magenta in color Positive

B. EFFECT OF pH B2. Mint green in color Positive

B3. Almost transparent with a shade of Positive

light blue

C1. Black solution with heavy digestion Positive

C2. black solution with average digestion Positive

C. EFEFCT OF ENZYME CONCENTRATION
C3. Black solution with slight digestion Positive

DISCUSSION

The researchers used salivary amylase in the first experiment to investigate how variations in
this enzyme’s concentration influence the rate of reaction with starch. Salivary amylase is a digestive
enzyme contained in saliva that breaks off maltose molecules and degrades starch. This enzymatic
response allows water molecules to be absorbed. During the experiment, there was no reaction in the
starch solution. On the other hand, the one that contains saliva, changed in color (yellow) after boiling.