Result

Biological Molecule Test name Reagants Procedure

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1) Prepare the food sample. Iodine color

Starch Iodine test Iodine solution 2) Add 1 - 2 drops of iodine solution (Red & yellow & brown) Blue and dark blue

1) Prepare sample (3 - 5ml)

Protein Biuret test Biuret (NaHO) (CuSO4) 2)Add biuret reagents (Same volume) Blue Shade of purple

1) Prepare sample (3-5ml) Milky

2) Add ethanol (Same volume) Remain clear
Ethanol or
Lipid Ethanol emulsion test 3) Shake &
Water cloudy white
4) Add water (Same volume) Transparant emulsion
5) Shake

1) Prepare sample (3-5ml) Green -> Yellow -> Orange

Benedict test Blue or
Reducing sugar Benedict's solution 2) Add Benedict's solution (Same volume) -> Brown -> Red
for reducing sugar deep blue
3) Heat in waterbath (80° - 100°) for 3-5 min -> Brick red

1) Negative for reducing sugar test

Benedict's solution 2) Prepare fresh sample (3-5 ml)
+HCl 3) Add HCl (1 drop / ml sample) Green -> Yellow -> Orange
Benedict's test for Blue or
Non-reducing sugar +NaHCO3 -> Brown -> Red
non-reducing sugar 4) Heat (80° - 100°) for 3-5 min deep blue
or -> Brick red
NaOH & PH paper 5) Cool & neutralise (Base until PH ± 7)
6) Do Benedicts' test for reducing sugar