AE-600N PHOTOMETER

SERVICE MANUAL

ERMA INC. JAPAN VER0501

 AE600N SERVICE MANUAL

Contents
1. Introduction------------------------------------------------------------------------2

2. Integral structure-----------------------------------------------------------------3

3. CPU Board--------------------------------------------------------------------------5

4. POW&DRV Board-----------------------------------------------------------------12

5. KEYB&LCD Board---------------------------------------------------------------16

6. PRE Board-------------------------------------------------------------------------19

7. Optical system structure------------------------------------------------------22

8. Configuration of power supply ---------------------------------------------23

9. Machine self-check--------------------------------------------------------------25

10. Malfunction analysis----------------------------------------------------------- 28

11. Part replacement----------------------------------------------------------------- 30

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 AE600N SERVICE MANUAL

1. Introduction

AE600N PHOTOMETER is new developed product adopt advanced electronic and optic
technology & refer to the different characteristic from existing same instrument. In the process of
design, the designer try hard to make hardware steady & safe, software simple & convenience.
During trial-testing in the hospital, it receives a lot of appraisement.
AE600N PHOTOMETER with comprehensive report list printout, can save 2048 testing
results each time and 100 samples maximum. Each sample with 256 test results.
AE600N PHOTOMETER is equipped with big capacity non-volatile storage which can set
96 analysis divided into 6 groups. Each group with 16 analyses.
AE600N PHOTOMETER measuring time can reach 66 minutes (for kinetic measurement),
especially useful for scientific research and pharm. analysis.
AE600N PHOTOMETER adopts module structure. Each component can fulfill a single and
complete function. Such structure is useful for maintenance. The replacement of pump tube, print
paper, lamp and so on consume material can be finished outside instrument, not need to open the
instrument.
All the parts are installed on the steel base for more stable, anti-shaking, electro magnetic
shielding advantage. Power supply with switch power supply, with low electric consuming, wide
range of supply voltage.
AE600N PHOTOMETER can check on the working status of instrument at any time.

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2. MAINFRAME CONSTRUCTION
AE600N PHOTOMETER hardware construction:
* power supply
* CPU
* Driver circuit
* Measuring circuit
* Control circuit
* Input & output circuit
* Optic and temperature control system
Total six parts:
1. Power supply:
Switch power1: supply 3 channel power: +5V for logic circuit and inner printer.
+9.5V for PELTIER positive power, pump motor and filter selection motor driver.
-9.5V for PELTIER negative power.
Switch power2: supply 3 channel power: +12V for lamp power.
-14V for LCD Driver POWER SUPPLY.
-12V for fan.
2. CPU: CPU system is in combination with 78E58B(a product of 8031 family) and periphery chip.
DS1230Y is a 32K NV SRAM, use for storage the data that stored permanently and data need
not stored permanently, such as parameters of analysis and results etc. DS12C887 is a real time
clock, use to supply date and time for instrument. 82C55 is a expanding parallel I/O port, use to
control keyboard, filter select, temperature select.
3. Driver circuit: drive step motors (pump motor and filter selection motor) ,PELTIER and lamp.
4. Measuring circuit: amplify the signal output from photo sensor, covert linear signal to logarithm
signal, control the selection of filter, A/D converter ICL735 convert analogy signal (such as
linear signal of photo sensor, log signal, temperature, +5V, +12V) to digital signal.
5. Control circuit: P1 port of CPU and 82C55 I/O port, temperature select, keyboard reading.
6. Input and output circuit: parameters result and other data display on the LCD, and printer. The
results of sample and control sent to RS232 port as requirement. The operation command input
by keyboard.
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7. Light system: 12V/20W HALOGEN Lamp is the light source. Lens focus light on selected filter
and filter light to monochromatic light, monochromatic light pass through the measuring liquid,
the photo sensor convert light signal to electronic signal..
8. Temperature system: composed by PELTIER and NTC thermal resistance, driver circuit.

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3. AE600 CPU BOARD

Instrument built in 4 circuit board: CPU, POW&DRV, PRE, KEYB&LCD.

CPU Board
78E58B+74HC573+DS1230Y+74HC139(2)+82C55+DS12887+74HC32+74HC574 compose
CPU system.
 2 74HC139 split the external IO space 0000-FFFFH to 3 grade: 1: 0000-7FFFH/
8000H-FFFFH, 2: 0000-1FFFH/ 2000-3FFFH /4000-5FFFH/ 6000-7FFFH 3:6000-67FFH/
6800-6FFFH/ 7000-77FFH/ 7800-7FFFH.
CS1/=0000-7FFFH
CS2/=8000-FFFFH DS1230Y, 32K NV SRAM
CS3/=0000-1FFFH LCD
CS4/=4000-5FFFH NO USE
CS5/=2000-3FFFH 82C55
CS6/=6000-7FFFH
CS7/=6000-67FFH OUT PRINTER
CS8/=7000-77FFH INNER PRINTER
CS9/-6800-6FFFH DS12C887
CS10/=7800-7FFFH 74HC574
 Address of DS1230y is 8000-FFFFH, use for storing data that need or not need stored
permanently.
 Address of DS12c887 is 6800-6FFFH, it is a real time clock, use to supply date, time signal
for CPU. With battery built in.
 Address of 82C55 is 2000-3FFFH, it is a 24 bit expanding parallel port. PA0, PA1, PA2,
PA3, PA4, PA5, use for read data of keyboard. PA6, PA7, PC0, PC1, PC2, PC3, PC4, PC5,
PC6, PC7, use for read data of A/D converter ICL7135. the data range of A/D converter
output is 00000-19999. PB0, PB1 control temperature select. PB2, PB3, PB4, control AV
adjustment.
 Address of inner printer is 7000-77FFH. Address of outer printer is 6000-67FFH.
 Address of LCD is 0000H-1FFFH.
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 Address of 74HC574 is 7800-7FFF, it is a 8 bit expanding parallel port for output. D0, D1,
D2, D3 control pump motor. D4, D5, D6, D7 control filter selection motor.
 P1.0 of 78E58B is the busy signal of outer printer.
 P1.1 of 78E58B is the busy signal of inner printer.
 P1.2, P1.3 used to control temperature selection.
 P1.4 used to detect the optical switch(initial position of filter).
 P1.5, P1.6, P1.7 use for select A/D channel.
 P1.7 of 78E58B use for control buzzer.
 P3.0, P3.1(RXD, TXD) is RS232 port.
 P3.2 is connected to SQW of DS12C887, is a 2 Hz square wave signal, supply timer signal
for measuring.
 P3.3 is connected to ASP. Button.
 P3.4 use to control buzzer.
 P3.5 is connected to BUSY of ICL7135 A/D converter.

CPU Board connects other board by connector:

*J1 26P IDC flat cable connector, connect outer printer. This is a standard parallel printer port,
the pins configuration is:
J1.1 STB/ J1.2 NC
J1.3 D0 J1.4 NC
J1.5 D1 J1.6 NC
J1.7 D2 J1.8 NC
J1.9 D3 J1.10 GND
J1.11 D4 J1.12 GND
J1.13 D5 J1.14 GND
J1.15 D6 J1.16 GND
J1.17 D7 J1.18 GND
J1.19 NC J1.20 GND
J1.21 P1.0(busy) J1.22 GND
J1.23 GND J1.24 GND
J1.25 NC J1.26 NC
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 AE600N SERVICE MANUAL

*J2 26P IDC flat cable connector, connect inner printer. This is a standard parallel printer port,
the pins configuration is:
J2.1 STB/ J2.2 NC
J2.3 D0 J2.4 NC
J2.5 D1 J12.6 NC
J2.7 D2 J2.8 NC
J2.9 D3 J2.10 GND
J2.11 D4 J2.12 GND
J2.13 D5 J2.14 GND
J2.15 D6 J2.16 GND
J2.17 D7 J2.18 GND
J2.19 NC J2.20 GND
J2.21 P1.1(busy) J2.22 GND
J2.23 GND J2.24 GND
J2.25 NC J2.26 NC

*J3 26P flat cable connector, connect board KEYB&LCD, the pins configuration is:
J3.1 +5V J3.2 +5V
J3.3 PA.4 J3.4 PA.5
J3.5 PA.2 J3.6 PA.3
J3.7 PA.0 J3.8 PA.1
J3.9 GND J3.10 GND
J3.11 D7 J3.12 +5V
J3.13 D5 J3.14 D6
J3.15 D3 J3.16 D4
J3.17 D1 J3.18 D2
J3.19 A6 C/D OF LCD J3.20 D0
J3.21 WR/ J3.22 RD/
J3.23 - 12V J3.24 CS/ 0000-1FFFH
J3.25 GND J3.26 GND.
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 AE600N SERVICE MANUAL

*J4 CH3.96-4P connector, connect to DC power supply, pins configuration is:

J4.1 +5V J4.2 GND
J4.3 GND J1.4 +5V

*J5 CH2.54-2P connector, connect to ASP. button.

J5.1 GND J1.2 BUTTON

*J6 IDC 10P flat cable connector, connect to RS232 port, the pins configuration is:
J6.1 NO USE J6.2 RXD
J6.3 TXD J6.4 NO USE
J6.5 GND J6.6 NO USE
J6.7 NO USE J6.8 NO USE
J6.9 NO USE J6.10 NO USE

*J7 IDC 20P flat cable connector, connect to POW&DRV board, the pins configuration is:
J7.1 DGND J7.2 +5V
J7.3 P1.2 SELCET A OF 4052 J7.4 P1.3 SELECT B OF 4052
J7.5 D0 J7.6 D1
J7.7 D2 J7.8 D3
J7.9 D4 J7.10 D5
J7.11 D6 J7.12 D7
J7.13 -12V J7.14 +12V
J71.5 +5V J7.16 -5V
J7.17 P1.4 OPT SWT J7.18 VT(TEMPERATURE)
J7.19 AGND J7.20 AGND
D0---D7 are 8 Bit output of 74HC574, D0-D3 use to control pump motor, D4-D7 use to control
filter motor.
VT is temperature signal output by a resistor circuit combined by a NTC thermo-resistor and a
6.8K fixed resistor.

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*J8 CH2.54-5P connector, connect to PRE board, the pins configuration is:
J8.1 AGND J8.2 -5V
J8.3 V LINEAR J8.4 +5V
J8.5 AGND
VLINEAR is the linear output signal of preamplifier.
*J9 is useless for current version, but reserved for future use.
*J9 2P CH2.54 connector, use to test reference voltage of A/D converter when adjust it. The
range is 1.0000+ 0.0010V.
74HC32 compose printer’s address chip select and write signal to strobe signal of printer port.
CD4051 8-1 multiplexer, use to select signal to be A/D converted from the signal as: +5V,
+12V, GND, TEMPERATURE, LINEAR OUT, LOG OUT.
ICL7135 and other RC component compose the A/D converter circuit, LM385-2.5 provide
reference voltage for ICL7135. Crystal oscillator 500k provide clock signal for ICL7135.
Reference voltage of ICL7135 is 1.000V, so range of input signal is 0.000-1.999V, the output of
ICL7135 is 00000-19999. The output of A/D converter is read to CPU by 82C55.
There 6 trimmers on the CPU board:
VR1 use to adjust display temperature, the displayed temperature increase when turn this
trimmer clockwise, otherwise the displayed temperature decrease.
VR2, VR3 use to adjust out put of linear—log converter.
VR4 use to adjust the bias of amplifier IC17(CA3140E).
VR5 is useless for current version, but reserved for future use.
VR6 use to adjust reference voltage of ICL7135, reference voltage increase when turn this
trimmer clockwise, otherwise the voltage decrease. The reference voltage of ICL7135 is in the
range:0.999--1.001V.
There are 3 jumpers on CPU board, P1 of SW1 connect to P2 of it. SW2 and SW3 use to
measure Voltage when adjust display temperature. When adjust display temperature, connect
2-3 of SW2 by a jumper, adjust VR1, make sure V12=0.6×V23. V12 is voltage between P1 and
P2 of SW3, V23 is voltage between P2 and P3 of SW3.
All the adjustments are done well in factory before instrument delivery, no more
adjustments are needed.

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 AE600N SERVICE MANUAL

SCHEMATIC DIAGRAM

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 AE600N SERVICE MANUAL

CONFIGURATION OF CPU BOARD

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 AE600N SERVICE MANUAL

4. AE600 KEYB&LCD Board

Key board/display IO board: with LCD bus and backlit power , and 45 keyboard encoder
74C923 on this board.
● J1 26P IDC flat cable connector, connect to J3 of CPU board. The pins configuration is:
J1.1 +5V J1.2 +5V
J1.3 PA.4 J1.4 PA.2
J1.5 PA.3 J1.6 PA.4
J1.7 PA.0 J1.8 PA.1
J1.9 GND J1.10 GND
J1.11 D7 J1.12 +5V
J1.13 D5 J1.14 D6
J1.15 D3 J1.16 D4
J1.17 D1 J1.18 D2
J1.19 A6 C/D J1.20 D0
J1.21 WR/ J1.22 RD/
J1.23 -12V J1.24 CS/
J1.25 GND J1.26 GND
● J2 9P connector, connect to keyboard. The pins configuration is:
J4.1 SL0 J4.2 SL1
J4.3 SL2 J4.4 SL3
J4.5 SL4 J4.6 DL0
J4.7 DL1 J4.8 DL2
J4.9 DL3
● J3 40P IDC flat cable connector, connect to LCD, the pins configuration is:
J3.1 –J3.2 FM GND
J3.3 –J3.4 DGND
J3.5 –J3.6 D+5V
J3.7 –J3.8 VD -14V(driving power)
J3.9 –J3.10 WR/
J3.11 –J3.12 RD/
J3.13 –J3.14 CS/
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 AE600N SERVICE MANUAL

J3.15 -J3.16 A6 C/D

J3.17 -J3.18 RESET
J3.19 -J3.20 D0
J3.21 -J3.22 D1
J3.23 -J3.24 D2
J3.25 -J3.26 D3
J3.27 -J3.28 D4
J3.29 -J3.30 D5
J3.31 -J3.32 D6
J3.33 -J3.34 D7
J3.35 -J3.36 GND
J3.37 -J3.38 GND
J3.39 -J3.40 V0
Note: J3.2is connected to J3.1, J3.4is connected to J3.3, …… ,so on.
Note: After changing of LCD& KEYB board or LCD display, please adjust VR1 on LCD&KEYB
board, make sure the contrast of LCD display is comfortable.

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 AE600N SERVICE MANUAL

SCHEMATIC DIAGRAM OF KEYB&LCD BOARD

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 AE600N SERVICE MANUAL

CONFIGURATION OF KEYB&LCD BOARD

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5. POW&DRV BOARD
POW&DRV BOARD is a driver board of PELTIER, step motors. Connect with CPU board,
DC power supply, and temperature control system.
T1 7912 is a -12V voltage regulator, regulate –14V to –12V.
T2 7805 is a +5V voltage regulator, regulate +9.5V to +5V.
T3 7905 is a -5V voltage regulator, regulate –9.5 to –5V.
T4 TIP 122, T5 TIP 127 is a pair of Darlington, use to drive PELTIER.
T6, T7, T8, T9 IRF530, are four MOS transistor, use to drive pump motor.
T10, T11, T12, T13 IRF530, are four MOS transistor, use to drive filter selection motor.

● J1 20P IDC flat cable connector, connect to CPU board, the pins configuration is:
J1.1 DGND J1.2 +5V
J1.3 P1.2 SELCET A OF 4052 J1.4 P1.3 SELECT B OF 4052
J1.5 D0 J1.6 D1
J1.7 D2 J1.8 D3
J1.9 D4 J1.10 D5
J1.11 D6 J1.12 D7
J1.13 -12V J1.14 +12V
J11.5 +5V J1.16 -5V
J1.17 P1.4 OPT SWT J1.18 VT(TEMPERATURE)
J1.19 AGND J1.20 AGND
D0---D7 are 8 Bit output of 74HC574, D0-D3 use to control pump motor, D4-D7 use to control
filter motor.
VT is temperature signal output by a resistor circuit combined by a NTC thermo-resistor and a
6.8K fixed resistor.

 J2 CH3.96-6P connector, connect with DC power supply, the pins configuration is :

J2.1 GND J2.2 +12V
J2.3 -14V J2.4 +9.5V
J2.5 -9.5V J2.6 GND

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 AE600N SERVICE MANUAL

 J3 CH2.54-4P connector, connect to optical switch.

J3.1 + OF LED J3.2 GND
J3.3 E OF TANSISITOR J3.4
 J4 CH2.54-6P socket, connect with pump motor.
J4.1 + 9.5V J4.2 Ф1
J4.3 Ф2 J4.4 Ф3
J4.5 Ф4 J4.6 +9.5V
 J5 CH2.54-6P socket, connect to filter selection motor.
J5.1 + 9.5V J5.5 Ф1
J5.3 Ф2 J5.5 Ф3
J5.5 Ф4 J5.5 +9.5V

 J6 CH3.96-4P socket, connect to temperature control

J4.3 RNTC J4.4 +12V
J4.5 VPELTIER J4.6 GND

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 AE600N SERVICE MANUAL

SCHEMATIC DIAGRAM OF POW&DRV BOARD

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 AE600N SERVICE MANUAL

CONFIGURATION OF POW&DRV BOARD

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 AE600N SERVICE MANUAL

6．AE600 PRE BOARD

PRE is pre-amplifier of photo signal. On this board, photo sensor convert optic signal to
electronic signal, amplifier amplify the output of photo sensor.
● J1 CH2.54-5P connector, connect to CPU BOARD, the pins configuration is:
J1.1 AGND J1.2 -5V
J1.3 VLINEAR J1.4 +5V
J1.5 AGND

There are 1 trimmer on this board, use to adjust bias of amplifier.

Note: all the adjustment is done well in factory before delivery, no any re-adjustment is necessary.

SHEMATIC DIAGRAM OF PRE (Refer to SCHEMATICA DIAGRAM OF KEYB&LCD)

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CONFIGURATION OF PRE BOARD

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AE600N SERVICE MANUAL

7. Configuration of power supply

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8. Optical and temperature control system structure

The structure of optic system as following graph:

12V/20W halogen lamp is light source. Heat isolator absorb infrared light with
wavelength >1000nm. Lens focus light on filer, and light change to monochromatic light.
Monochromatic light pass throw measuring liquid, and shine on optical sensor. The optical sensor
transfer light signal to electro-signal, and amplified by pre-amplifier.
There are 8 positions of filter on filter wheel, and for current version, 6 filters built in(340nm,
405nm, 505nm, 546nm, 578nm, 630nm.). Step motor drive filter wheel rotate according to software
command, and optical position switch detect the initial position of filter wheel.
When changing filter, the filter wheel rotate clockwise to the initial position, and then rotate steps as
number stored in memory for this filters. When filter is selected, instrument adjust AV(amplify
Value) to the value stored in memory.

Temperature control system composed by Peltier , NTC thermal resistance and relevant drive circuit.
The thermal resistance Rx=R25*{EXP[(K*)(1/Tx-1/T25)]}, R25=52.3KΩ±1%, K=4041.
PELTIER cool or heat the cuvette housing according to the drive voltage.
The trimmers VR1, VR2, VR3 VR4 on POW&DRV board supply the reference voltage of 25, 30,
32, 37℃ separately, they are 1.10V – 1.30V, 1.60V – 1.80V, 4.80V – 2.00V, 2.10V – 2.30V, increase
the reference voltage could raise the cuvette housing temperature, decrease the reference could
reduce the temperature.
The trimmer VR1 in board CPU are for adjusting temperature display.
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9. Instrument check and malfunction analysis of instrument
The instrument has a self check function CHE for inspecting working status of instrument.
In the main menu, select CHE and press confirm:

Test Cal. Par. Rep. CHE.

Press R/L key select function

Press ASP. key wash flow cell.
2004 03/20 10:30:25
S/N:050403008

The instrument would display as follows:

CHE
+5V=5.0234 L12V=12.134
CH AV POS. LIN. LOG.
1 65 23 12000 02500
T=37 Pump Set:
PRN: ON
2003 03/20 14:06:55

In this display, +5V power voltage is showed behind +5V. Range is 4.8000 to 5.2000.
Move cursor down wards, L12V represents the lamp voltage. Range is 11.800 to 12.200.
Move cursor down wards, the number displayed under CH represents selected channel No., press
number keys 1,2,3,4,5,6 can select channel 1 (340nm), channel 2 (405nm), channel 3 (505nm),
channel 4 (546nm), channel 5 (578nm), channel 6 (630nm).
The value displayed under AV is amplify value of selected channel, AV of selected channel can be
modified by press number key 8 or 9. Press number key 8 once, AV increase one unit, and press
number key 9, AV decrease one unit. AV value is expressed as 0.1, for example displayed AV is 78,
the real amplify value is 7.8. The maximum of AV is 109, and the minimum is 10.
The value displayed under POS. is the position of selected filter, it is the number of steps motor to
rotate from initial position to put selected in optical path. Press ZERO key or cursor key ↓ can
change POS. Press once, POS increase or decrease one step.

Move cursor down wards, adjust temperature:

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 AE600N SERVICE MANUAL

CHE
+5V=5.0234 L12V=12.134
CH AV POS. LIN. LOG.
1 65 23 12000 02500

T=37 37.1 Pump Set:

PRN: ON
2003 03/20 14:06:55

The first displayed value behind T= is temperature setting, the second is cuvette housing
temperature. Select T=, press ENTER, then press 0, 1, 2, or 3 to select temperature 25, 30, 32, 37.
After selecting, press ENTER key, the temperature would be adjusted to setting one.
The temperature adjuster is on POW&DRV board，VR1, VR2, VR3, VR4，adjust cuvette housing
temperature of 25, 30, 32, 37℃ correspondingly. For example, press number key select 37℃, insert a
mercury thermometer with range 0-50℃ and resolution 0.1℃ into temperature testing adapter with
about 100μL water in it, wipe off the over flow water on surface of adapter, and then put adapter
with mercury thermometer into cuvette housing, read cuvette housing temperature from
thermometer. Adjust VR4, the temperature read from thermometer will change slowly. Adjust VR4,
make sure that the difference of temperature read from thermometer and the temperature setting is
little than 0.2℃.
Note: Each time, after adjusting temperature, wait 2-3 minutes for temperature stable.
The displayed temperature can be modified by software:
1. Before modify display temperature, adjust cuvette housing temperature correctly firstly.
2. Move cursor to L12V display, wait 1-2 minutes for display stabling. Press ZERO key,
instrument will record data of L12V automatically, and the display of L12V will set to 12.000.
3. Move cursor to temperature setting, and set temperature to25℃, watch the change of display
temperature. Wait about 2-3 minutes, display temperature will be stabling, and then press
ZERO key, instrument will record the data of 25℃ automatically, and the display temperature
will change to 25.0℃.
4. Set temperature to 37℃, watch the change of display temperature. Wait about 2-3 minutes,
display temperature will be stabling, and then press ZERO key, instrument will record the data
of 37℃ automatically, and the display temperature will change to 37.0℃.
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5. Remove the thermometer and adaptor, install cuvette and tube kit, temperature display
modification is finished.
Move cursor downwards, select PUMP SET, press ENTER to display the last one pump setting
value. To process pump calibration, press ASP. key to aspirate 1 ml distilled water, press ASP. key
again to finish pump calibration. The new calibration value would be displayed.
Move cursor down wards, select PRN. Behind the PRN, there is ON or OFF, which shows the
status of automatic printing. If the status is ON, the instrument would print out the measuring result
automatically. If the status is OFF, the instrument would not print out automatically. Press ENTER
key, the automatic printing status would switchover and print self test report:
PRINTER TEST OK!
PRINTER TEST OK!
Move cursor downwards to choose date and time, press ENTER key to set date and time. Press left
and right key to select modified place. After setting, press ENTER key again.
The sequence of date and time as follows:
Year month/date hour: minute: second

After self test, press EXIT return to main menu.

Here, press PRINT key, inner printer print out screen display.

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9. malfunction analysis

Malfunction Checking Solution

No display after switch on Check power supply Connect power supply properly
Check fuse Replacing new fuse.
After replacing pump tube, Pump tube too short Replacing a standard tube
pump would not rotate when
aspirating
After replacing pump tube, Pump tube too long Replacing a standard tube
pump would rotate when Pump tube do not fixed on rack Fix the pump tube in rack.
aspiration, but the liquid could Pump tube fixed inversely Reinstall the pump tube.
not been aspirated
The outer printer could not Check power supply and Connect power supply and
make printouts communication cable communication cable
After replacing aspirating tube, The aspirating tube is not Screwed tight aspirating tube
bubbles arise continuously in screwed tightly
flow cell
The instrument has not reaction Instrument is under testing Please wait for finishing the
after pressing corresponding Instrument is saving parameter undergoing work.
key Instrument is printing
Temperature rise continuously, Take off plug J3 of board If thermal resistance is
could not reach setting one POW&DRV, testing thermal defective, replacing new one
resistance, normal value within
30K-80K
Temperature could not rise or Take off plug J3 of board If PELTIER is defective,
descend POW&DRV, testing both ends replacing new one
of PELTIER, bi-directional
testing should conduct in
normal status
Peristaltic pump do not rotate The screw in pump head loose Screw down the three screws in
pump head
Step motor do not rotate Check plug J4 in board Connect plug J4
POW&DRV
Absorbance displayed unstable Check if there is air bubble in Empty the liquid in flow cell.
flow cell. There would be Re-aspirating could remove the
influence even very small bulb bubble.
The bubble in the flow cell There is lip or other dust in Washing flow cell with
could not be removed. flow cell detergent
The flow cell leak Screw down the connector of
Pump tube are worn out flow cell
Replacing new pump tube
Each channel value could not Position of lamp is changed Adjust the position of lamp
reach requirements when self The bulb is aging Replacing new lamp
testing Filter is aging or mildew Replacing filter
Cuvette has not been taken off Taking off flow cell
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After replacing lamp, each The position of lamp is not Adjust the position of bulb
channel value could not reach adjusted properly Replacing the lamp supplied by
requirements The model of lamp is wrong manufacture
It is very important for ensuring the normal use of instrument to make routine maintenance as per
requested.
 Each day, after testing, washing cuvette for one minute by distilled water.
 Taking off pump tube from the rack after rinse.
 Cover the instrument with dust proof cover after switch off.
 Each week, drop 3 to 5 drops of detergent in 50ml distilled water, rinsing cuvette and tube.
After rinse, washing flow cell with 50ml distilled water.
 Every 4-6 months, replacing pump tube or replacing it when necessary.
 Check print paper regularly, make replacement when necessary.
 Ensure the cleaning of instrument appearance.

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11. Parts Replacement

More detailed descriptions about parts replacement refer to instruction of part replacement, a
windows words file in CD, with photograph of parts replacement.
1. Replacing pump tube
Taking off pump tube from pump head, hold one end of metal connection tube, pull out metal hoop
from another end with strength, then pull out the pump tube from metal connection tube. Taking off
another end of pump tube in the same way.
Insert one end of new pump tube to metal connection tube, penetrate two metal hoops to pump tube.
Please note one end of hoop with bigger inside diameter should aim at metal connection tube.
Interlink metal connection tube with one metal hoop. Then insert another end of pump tube to the
other metal connection tube and interlink with metal hoop.
The pump calibration is required after replacing new pump tube.
2. Replacing print paper
Clamp gap at the bottom of printer with thumb of right hand, taking off the cover board upwards
with strength. Taking out the used paper shaft. Tear the new print paper, cut the paper head into
triangle, penetrate paper lead shaft, put the scroll in, put the paper lead shaft on the rack. Insert the
paper head into the paper feed slot which is under the ribbon. Press paper feed key to feed paper.
Pull the paper head slightly into right way, penetrate the paper cutter of cover board and cover the
board.
4. Replacing lamp:
The rated life for lamp is 2000 hours, the performance would descend after long time usage. It is
necessary to replace lamp.
Note: The surface of lamp is very hot, not touch it with hand. Please wait for some minutes to
cool down after switch off instrument power.
Remove lamp chamber cover, unscrew 2 screws fix lamp holder to optical assembly, take off lamp
holder from instrument. Loosen 2 screws fix lamp to lamp holder, take off old lamp, and put on new
one. Drive tight 2 screws to fix lamp to lamp holder, not tight thoroughly, let the lamp position can
be adjust by hand. Adjust the lamp position, let the filament of lamp in center of lamp holder, and
then drive tight 2 screws thoroughly.
Drive tight 2 screws to fix lamp holder on to optical assembly, not tight thoroughly, let the position
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of lamp holder can be adjusted by hand. Check channel value in self check menu, adjust lamp
holder position, make sure the channel value is the maximum but not exceed 19000. Drive tight 2
screws thoroughly.
Please pay attention not to touch the surface of lamp bulb. Use clean textiles rub the surface of lamp
if it is not clean.

5. Replacing pump head

The pump head is fixed in motor axle with three screws, loose screws, pull out pump head upwards.
Screw on the screws in new pump head, but the screws should not be drive down. Insert new pump
to motor axle, adjusting the position of pump head to make the bottom of pump head 1mm away
from instrument case, then screw down three screws. After screw down pump head, when rotating,
stop its rotation by hand, this time, there is no slide between motor shaft and pump head.

6. Replacing flow cell

New flow cell is supplied together with aspiration tube, connection tube, pump tube and waste tube.
For replacing flow cell, just taking out old one and insert new one. Please note the light cross
aperture of flow cell should be the same direction with the one of flow cell chamber. Using
detergent to wash flow cell and tube line after replacement.

7. Replacing fuse tube

The fuse tube is in the bottom half of outlet in the back of instrument, draw out the fuse tube slot
and take out the old fuse tube, replace new one and insert the slot.

8. Replacing CPU board

The CPU board installed in the bottom panel of instrument, take off all cables and wires connected
to CPU board, and unscrew 4 screws fix CPU board to bottom panel. Take off the old CPU board,
replace new one, drive tight 4 screws fix CPU board to bottom panel, connect cables and wires.
Note: After replacement, re-adjust temperature is required.
Note: The S/N of instrument is programmed in CPU chip 78E58B. If the S/N is not included in
the part order, no 78E58B built in CPU board. Please take of it from old board and put on to
new board.
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Note: After CPU board replacement, the parameters such as AV, POS, temperature, pump
setting, and parameters of items will be changed, please modify or recalibrate, or take off
DS1230Y from old board and put on to new board.

9. Replacing POW&DRV board

The POW&DRV board is in the backside of instrument, take off cables and wires connected to it,
and unscrew 4 screws fix it to instrument case, take off old board and put on new one, drive 4
screws to fix board to instrument case. Connect cables and wires to board.
Note: After POW&DRV board replacement, must adjust the cuvette housing temperature.
10. Replacing KEYB&LCD board
The KEYB&LCD board is in the top of instrument, remove all the cables and wires connected to it,
unscrew 4 screws fix it to instrument case. Take off the old KEYB&LCD board, replace new circuit
KEYB&LCD board, drive tight 4 screws to fix board to instrument case, connect cables and wires.
Note: After replacing the new KEYB&LCD board, adjusting VR1 to make the contrast of
LCD suitable.
11. Replacing PRE board
The PRE board is installed in one black aluminum box, unscrew 4 screws fix black box to optical
assembly, take off black box. Replace new PRE board.
Note: Before installing PRE board, clear away mess in the light sensitive surface of cell. After
replacement, please adjust AV in self check menu to make sure the channel value is within
range 10000-18000.
12. Replacing PELTIER
Loose four screws fix heat sink to optical assembly about 4mm, solder down PELTIER electrode,
pull out old PELTIER downwards and insert the new PELTIER in the same direction. Making the
top of PELTIER align with the top of cuvette housing and screwing down four screws, but not too
tight. Press the flow cell chamber to bottom and screw down four bolts, then solder PERLTIER
electrode to the former position.
Note: Spread heat conduction silicone grease equably before install new one. During the process of
screwing down screws, please use strength equably to avoid damaging PELTIER.
13. Replacing power supply
Power supply 1, power supply 2 are all fixed on bottom panel, take off the connected wire, loose the
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fixed bolts, after replacement, screw down the bolt. Connect 220V power supply, adjusting voltage:
the power supply 1+5V output is within the range of 5.000-5.010V, power 2 +12V output is within
the range of 11.9000-12.000. Connect the wire as the previous position.
Note: switch off instrument power and remove power cord when replacing power supply.

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