NAME:__________________________________ SCORE:_____________

DATE:___________________
INTERNSHIP MONTHLY EVALUATION EXAM
THIRD EVALUATION EXAM
CLINICAL MICROSCOPY
A. Answer the following questions accordingly.
1. Identify what type of urine specimen is preferred for the given tests.
A. Glucose determination C. Monitoring Insulin Therapy
B. Catecholamines D. Addis Count
2. What causes uniform turbidity in the urine that cannot be cleared by filtration?
3. A stool sample was received in the CM section for Fecal Occult Blood Test. The patient has clinical findings,
highly suggestive of colorectal cancer. You performed the test and the result came out negative. What are the
possible reasons of false- negative results in FOBT?
A.
B.
4. Interpret the following SSA and Reagent Strip Results for Proteins.
A. (+)SSA & (-) RGT STRIP
B. (+)SSA & (+) RGT STRIP
C. (-) SSA & (+) RGT STRIP
5. Interpret the following Clinitest & Reagent Strip Results for Glucose.
A. (+) CLINITEST & (+) RGT STRIP
B. (-) CLINITEST & (1+) RGT STRIP
C. (+) CLINITEST & (-) RGT STRIP
D. (-) CLINITEST & (4+) RGT STRIP
6. Presence of this analyte in the urine is a result of defective carbohydrate metabolism found in Diabetes
Mellitus.

B.COMPUTATION
1. A specimen kept at 22degC contains 2 g/dL of protein and 1 g/dL of glucose has a specific gravity of 1.034 when
using a refractometer. Calculate the corrected reading.

C. MODIFIED TRUE OF FALSE. Write TRUE if the statement is true, if otherwise, change the underlined word to
make the statement right.
1. Squamous epithelial cells are the largest cells in the urine sediment, have abundant irregular cytoplasm with
eccentric nucleus.
2. Examination of urine for the detection of casts is performed using LPO magnification, observed under subdued
light because cast matrix has a low refractive index.
3. Presence of crystals in freshly voided urine is most frequently associated with concentrated specimens.
4. The most direct contact with a source of infection in the clinical laboratory is through contact with the patient
himself.
5. Universal Precaution states that all body fluids and moist substances should be considered potentially infectious.

D. ESSAY
1. Describe how to properly collect a 24-hour urine specimen?
2. Discuss how Urobilinogen is present in the Urine?

3. How does Watson- Schwartz Test differentiates Urobilinogen from Porphobilinogen?

4. What are possible errors that an unmixed urine specimen tested using reagent strip can cause?

5. All biological hazards in the laboratory should be discarded in a yellow bin. Is this rule having an exception
under the Clinical Microscopy Section?

Prepared by:
S.T. CUSTODIO, RMT
Clinical Instructor
NAME:__________________________________ SCORE:_____________
DATE:___________________
INTERNSHIP MONTHLY EVALUATION EXAM
THIRD EVALUATION EXAM
HISTOPATHOLOGY AND CYTOPATHOLOGY

A. Answer the following questions accordingly.

1. What is the most preferred type of urine specimen for cytopathology.
2. What are the components of Saccomano’s Fixative?
A. B.
3. What are the components of Eosin Azure 50 that is used in Pap Stain?
A. B. C. D. E.
4. What is the most commonly used blueing agent in histopathologic and cytopathologic staining?
5. The following are the main factors affecting the rate of fixation in a Histopathologic specimen. Give the desired
value of each factors to hasten the rate of fixation.
A. Hydrogen ion concentration D. Osmolality
B. Temperature E. Concentration
C. Thickness of section F. Duration of Fixation

B. CALCULATION
1. How many liters of stock formalin solution will be added to 7 liters of distilled water to produce 10% formalin
solution?

C. MODIFIED TRUE OR FALSE. Write TRUE if the statement is true, if otherwise, change the underlined word to
make the statement right.

1. Xylene can greatly affect the quality of impregnation in Histopathologic Techniques.

2. Staining of living cells is done by injecting the dye into any parts of the animal body.
3. The use of adhesives in the slides used for histopathology is essential for methods that require exposure of
sections to acids and alkalis during staining.
4. Mounting medium protects the stained section from deterioration due to oxidation.
5. The slide carrying the section to be mounted is taken from the last xylene bath with the forceps.

B. ESSAY
1. How could an adhesive affect the staining quality of a cytological smear? Name three adhesives that are
commonly used in a cytological smear.
2.
 3. Why is there a variation in the container of reagents that is use in the histopathology lab?

4. If airholes are found in the tissue block during trimming, what is the reason behind this? Give the corresponding
remedy.

5. If the tissue is insufficiently dehydrated, what are the possible problems that will happen and how will you
remedy the faults.

6. How will you properly dispose specimens in histopath laboratory?

Prepared by:
S.T. CUSTODIO, RMT
Clinical Instructor
NAME:__________________________________ SCORE:_____________
DATE:___________________
INTERNSHIP MONTHLY EVALUATION EXAM
THIRD EVALUATION EXAM
IMMUNOLOGY, SEROLOGY AND BLOOD BANKING

A. Answer the following questions accordingly.

1. What is the purpose of Bovine Serum Albumin in performing Rh Typing?
2. What does it mean if there is a presence of agglutination in performing Weak D typing?
3. What are the two stages of agglutination?
A. B.
4. On what phase of Antibody Screening procedure do we detect the presence of cold-reactive antibodies?
5. Give examples of the cold-reactive antibodies
A. D.
B. E.
C. F.
6. Direct Antiglobulin Test is used for the investigation of;
A. C.
B. D.

B. COMPUTATION
1. 7 units of Packed RBCs are needed for transfusion. Laboratory findings indicate that the recipient has antibodies to
the Fya and E antigens. Almost 66% of the general population is positive to Fya antigen and 29% is positive to the
E antigen. How many units of blood should be crossmatched in order to obtain compatible unit?

C. MODIFIED TRUE OR FALSE. Write TRUE if the statement is true, if otherwise, change the underlined word to
make the statement right.

1. The purpose of AHG in Weak D testing is to detect the presence of Rh antigen in the patient’s RBC.
2. All antisera should be stored at 24degC when not in use.
3. Antibody excess in serological tests will result to False positive result.
4. Th antibody titer is defined as the reciprocal of the highest dilution of the patient’s serum in which the antibody is
still detectable.
5. Serum for detection of antibodies should be drawn during the Acute phase of illness only.

D. ESSAY
1. Explain the necessary factors to consider in drawing blood to a patient who had undergone mastectomy.
 2. Why are positive and negative controls essential to the accuracy of a test result?

3. Draw the principle of the test indicated in the picture below. Give the reagents, and specimen that is used in the
test. What is the positive result in this test?

4. Given the following phenotyping results, answer the following.

CASE A: Anti-A Anti-B A1 cells B cells
4+ neg neg neg
1. Identify the phenotype.
2. What is the cause of the problem in the reverse method?

CASE B Anti-A Anti-B Anti-A1 A1 cells B cells

4+ neg neg 3+ 4+

3. Identify the phenotype.

CASE C Anti-A Anti-B A1 cells B cells

2+ 2+ mf neg 4+

4. Identify the phenotype.

5. When and how will you dispose a blood unit that is positive for transfusion transmissible disease?

Prepared by:
S.T. CUSTODIO, RMT
Clinical Instructor