BIOSTAT® A

Your professional start in cell cultivation

and microbial fermentation
BIOSTAT® A
BIOSTAT® A is an entry-level bioreactor |
fermenter designed for easy control of cell
growth and fermentation. It is therefore
ideal for educational purposes.
The control tower of the BIOSTAT® A
combines all features and functions for
measurement and control: Fast Load
pumps, aeration module and conveniently
located connections for utilities and sensors.
Featuring a compact footprint, the
BIOSTAT® A saves valuable space in your
laboratory.
 Simple and automatic
aeration system

Intuitive operation for easy control by

beginners and experienced users alike

Integrated recirculating chiller for

fermentation in any lab
Simple and Automatic Aeration Control Intuitive Operation of BIOSTAT® A
Even Outside a Laboratory
The new aeration system of the BIOSTAT® A provides continuous Thanks to the intuitive user interface, operating the BIOSTAT® A
automatic flow control over the full range of each gas used. is ideal for beginners, reduces operating errors and speeds up
As a result, BIOSTAT® A does not require any manual adjustment training. Operation using a tablet or smartphone is a mode
of flow meters and therefore eliminates problems with pulsed offered in the Advanced package and enables you to monitor
aeration. Setting up the bioreactor for use is straightforward: and control the BIOSTAT® A on the move – in the laboratory, in
Just connect the aeration tubing, configure the aeration profile, the office or even at home.
enter the DO setpoint – that’s it!

For cell culture applications, interfaces for four gases (air, O2, CO2
and N2) are available for DO and pH control. The microbial version
features two gas lines (air and O2) for DO control. Easy status checks for your
pH and DO probes
BIOSTAT® A is equipped with digital pH and
DO probes. These help you immediately
recognize whether you can use a probe for
the next cultivation batch. Moreover, the
moisture-resistant plug connector ensures
secure data transmission to the BIOSTAT® A
at all times.

Integrated Chiller
for Microbial Fermentation
Allows fermentation in any laboratory
and minimizes water usage
You won’t need to worry about finding a usage, and you will not require a water
suitable cooling water source for your supply line for cooling installed in your lab.
bio­reactor. Each BIOSTAT® A for microbial The only utility connections you need are
fermentation is equipped with a chiller electricity and gas supply to operate the
that effectively removes heat from your BIOSTAT® A.
culture. This reduces your cooling water
Simple and Automatic Aeration Control Intuitive Operation of BIOSTAT® A
Even Outside a Laboratory
The new aeration system of the BIOSTAT® A provides continuous Thanks to the intuitive user interface, operating the BIOSTAT® A
automatic flow control over the full range of each gas used. is ideal for beginners, reduces operating errors and speeds up
As a result, BIOSTAT® A does not require any manual adjustment training. Operation using a tablet or smartphone is a mode
of flow meters and therefore eliminates problems with pulsed offered in the Advanced package and enables you to monitor
aeration. Setting up the bioreactor for use is straightforward: and control the BIOSTAT® A on the move – in the laboratory, in
Just connect the aeration tubing, configure the aeration profile, the office or even at home.
enter the DO setpoint – that’s it!

For cell culture applications, interfaces for four gases (air, O2, CO2
and N2) are available for DO and pH control. The microbial version
features two gas lines (air and O2) for DO control. Easy status checks for your
pH and DO probes
BIOSTAT® A is equipped with digital pH and
DO probes. These help you immediately
recognize whether you can use a probe for
the next cultivation batch. Moreover, the
moisture-resistant plug connector ensures
secure data transmission to the BIOSTAT® A
at all times.

Integrated Chiller
for Microbial Fermentation
Allows fermentation in any laboratory
and minimizes water usage
You won’t need to worry about finding a usage, and you will not require a water
suitable cooling water source for your supply line for cooling installed in your lab.
bio­reactor. Each BIOSTAT® A for microbial The only utility connections you need are
fermentation is equipped with a chiller electricity and gas supply to operate the
that effectively removes heat from your BIOSTAT® A.
culture. This reduces your cooling water
BIOSTAT® A
It’s the little things ...

... that make your life easier ...

Fast Load peristaltic pumps

Install tubing in three seconds:
Open, insert tubing, close –
done! No more pinched fingers,
torn gloves or tubing slippage
in the peristaltic pump head.

Convenient data storage and

analysis
Save your process data directly
on the BIOSTAT® A; then easily
transfer this data to your PC or
laptop spreadsheet application
for convenient analysis.

Flexibility made easy

Select the type and size of your
culture vessel on the BIOSTAT® A
menu, and all settings including
PID parameters will be
automatically adjusted.

... and enable you to take the next step.

Higher cell densities with

fed-batch processes
The Advanced package version
of the BIOSTAT® A with fed-
batch control lets you achieve
higher cell densities than with
standard batch control. Easily
implement substrate profiles in
the menu to control an
external variable-speed pump.
Glass and Single-use
for More Flexibility – Every Day
The BIOSTAT® A is available with single-wall glass vessels in a choice of working volumes
of 1, 2 or 5 liters maximum. Whether you select our reusable UniVessel® Glass version or
our disposable UniVessel® SU unit with a maximum 2-liter working volume, you can use
the same system controller.

Both the UniVessel® Glass and the UniVessel® SU feature the classic stirred-tank design
to give you comparable results. The advantage: The same motor drives the stirrer of both
vessels so you can easily change over from reusable to single-use or vice versa.

UniVessel® Glass
−− Classic stirred-tank design for easy
scaling to larger volumes with all
characterization data available up
to 2,000 L
−− Proven sterile design and robustness
−− Also fits in small autoclaves, saving you
additional investment costs
UniVessel® SU
−− Easy handling as the culture vessel
is fully pre-assembled and sterilized:
no parts to mount, no cleaning – just
connect to get started
−− No room for an autoclave to sterilize
your culture vessel? No problem!
The UniVessel® SU is single-use, from
the vessel to the sensors
−− Eliminates preliminary setup and
post-cleaning procedures so you can
better meet your tight experimental
deadlines and utilize your time for more
important tasks
Multi-parallel Cultivation
for Faster Results ...
Perform screening studies efficiently with multi-parallel cultivation.
Screen your process variables and define the conditions for optimal cell growth
and enhanced protein expression. Download your trial data from the BIOSTAT® A to
your notebook, tablet or smartphone. For fast and accurate analysis of your data,
use our Chemometrics Toolbox installed on your notebook or PC.
... Powered by BioPAT ® MODDE and BioPAT ® SIMCA

Quick results with little effort

Our Chemometrics Toolbox assists you in efficiently optimizing key process parameters, such
as titer or cell density, so you will obtain meaningful results faster from your experiments.

DoE
BioPAT ® MODDE
Thanks to its intuitive user guidance, this
leading DoE software is the ideal tool for
beginners in experimental design planning
and performing trial runs, as well as
analyzing and optimizing media and
parameters for cultivation.
MVDA
BioPAT ® SIMCA
With BioPAT ® SIMCA, you can quickly
and easily compare the historical data of
different cultivation batches to identify
trends, correlations and dependencies.
The graphical user interface enables you
to select the software functions intuitively.
In addition, the Analysis Advisor guides
you through the whole analysis process
and generates easy-to-interpret graphs.

By performing only a few experiments,

you can quickly and easily determine the
relationship between process parameters
and the relevant target figures, such as
titer and product quality.
BIOSTAT® A – Technical Specifications
Base Unit
Control Tower
Weight 13.5 kg | 29.7 lbs.
Footprint bench surface (W + H + D) 200 + 495 + 300 mm | 7.9 + 19.5 + 11.8 in.
Required space incl. connectors, pump 320 + 495 + 475 mm | 12.6 + 19.5 + 18.7 in.
heads etc. (W + H + D)
Power supply 100 to 240 VAC 50/60Hz, 8 A
Housing Metal housing, coated
Operating Temperature 0 °C – 40 °C
Maximum relative humidity 80% for temperatures up to 31 °C decreasing linearly to 50% relative humidity at 40 °C
Motor drive – Maintenance-free
– Quiet direct drive
– 150 W
Motor rpm, 1 L glass: 30 – 1,400 rpm
direct coupling 2 L glass: 30 – 1,100 rpm
5 L glass: 30 – 800 rpm
2 L SU: 30 – 400 rpm
Interfaces – 2 + external signal inputs 4 - 20 mA
– Ethernet
– Potential-free alarm contact
– USB
– Interface to chiller
– Interface to external pump
Regulatory compliance CE, NRTL recognized by the U.S. OSHA (Occupational Safety and Health Administration)
System requirements for operation iOS 7 or higher; Android 4 or higher
by tablet and smartphone

Pump Module
Integrated Pumps
Quantity 3
Controller Fixed speed (regulated on|off)
Speed 43 rpm
Pump head Watson Marlow 114, Fast Load pump head
Flow rates ID 0.5 mm: 0.8 ml/min
(tube wall thickness 1.6 mm) ID 0.8 mm: 1.7 ml/min
ID 1.6 mm: 6.0 ml/min
ID 2.4 mm: 12.5 ml/min
ID 3.2 mm: 20.2 ml/min
External Pumps
Quantity 1 (only in the Advanced package)
Controller Regulated rpms
Speed Max. 200 rpm
Pump head Watson Marlow 120, Fast Load pump head

Process Control and Measurements

Probes and Controllers
Temperature, separate sensor
Temperature integrated in pH probe
DO probe, reusable
DO sensor, single-use
pH probe, reusable
UniVessel® Glass UniVessel® SU
Temperature control 0 – 40°C •
Display resolution 0.1°C
Temperature control 0 – 60°C •
Display resolution 0.1°C
Polarographic probe •
Digital communication with Control Tower
Range: 0 –100%
Display resolution: 0.1%
DO sensor patch •
Range: 0 –100% air saturation
Display resolution: 0.1% air saturation
Combination electrode, digital communication with •
Control Tower
Range: 2 – 12 pH
Display resolution: 0.01 pH
Probes and Controllers UniVessel® Glass UniVessel® SU
pH sensor, single-use pH sensor patch •
Range: 6 – 8 pH
Display resolution: 0.1 pH
Foam, alternative level Electrical conductivity sensor, stainless steel, •
ceramic insulated

Aeration Module
Gas inlet – Gas pressure 1.5 barg (21.76 psig), ±10%
– Gases: dry, oil and dust-free
– Gas inlet connections with hose barb for 6 + 3 mm (0.24 x 0.12 in.) reinforced tubing
Gas outlet – Gas outlet pressure: max. 0.8 barg (11.6 psig)
–O utlets to the culture vessel with hose barb for 3.2 + 1.6 mm (0.12 x 0.06 in.) silicone
tubing
Two-gas System for Microbial Fermentations
Continuous and automatic aeration control for air and O2
Gas flow control units Total of two; one per gas segment
Flow rates of the gas flow control Air and O2: 100 – 7,500 ccm
units (all vessel sizes)
Measuring and control accuracy of the ± 5% full scale
gas flow control units
Four-gas System for Cell Culture Application
Continuous and automatic aeration control for air, O2, N2 and CO2
Gas flow control units Total of four; one per gas segment
Flow rates of the gas flow control Air and N2: 10 – 500 ccm | O2 and CO2: 5 – 250 ccm
units (all vessel sizes)
Measuring and control accuracy ± 5% full scale
of the gas flow control units

Temperature Control Module

Cooling (only included in the BIOSTAT® A for microbial fermentation)
Chiller with automatic cooling water valves for recirculated cooling of the culture vessel and exhaust cooler via cooling finger
Weight 19.5 kg | 43 lbs.
Dimensions (W + H + D) 220 + 495 + 325 mm | 8.6 + 19.49 + 12.8 in.
Volume of cooling water tank 1.5 L
Power supply - BB-8822002: 230VAC, 50/60 Hz, T2.5 A
- BB-8822003: 115VAC, 60 Hz, T4A
Supply temperature 8°C ±2°C at an ambient temperature of 21°C
Cooling capacity 150 W at an ambient temperature of 21°C
International protection rating IP21 (protected from solid objects > 0.5 inch and vertically dripping water)
Cooling water hardness Max. 12 dH
Connection with cooling finger via quick-connect coupling
Heating
Temperature control with electric heating jacket for culture vessel
Electrical filter heating for exhaust air filter (only included in the BIOSTAT® A for cell culture applications)
Culture vessel temperature control See above
range
Heating power - 1 L Glass:   66 W
- 2 L Glass: 112 W
- 5 L Glass: 264 W
- 2 L Single-use: 132 W
UniVessel® Glass Culture Vessel
Autoclavable, single-walled glass vessel 1L 2L 5L
Material Borosilicate glass, stainless steel AISI 316L, EPDM
Total volume [L] 1.6 3 6.6
Working volume [L] 0.4–1 0.6-2 0.6-5
Cover ports 19 mm | 12 mm | 6 mm 3 | 2 | 6 3 | 2 | 9 3 | 3 | 8
Weight1 kg (lbs.) 6 (13.2) 7 (15.4) 12 (26.5)
Space requirements
[W + H + D in mm] 200 + 540 + 270 230 + 600 + 300 260 + 730 + 340
[W + H + D in inches] 7.9 + 21 + 10.6 9.1 + 23.6 + 11.8 10.2 + 28.7 + 13.4
Space requirements, reduced²
[W + H + D in mm] 480 + 410 + 300 530 + 510 + 410
[W + H + D in inches] 18.9 + 16.1 + 11.8 20.9 + 20.1 + 16.1
1
With stand and cover, without medium
2
Exhaust air cooler adapters for height reduction in the autoclave are available as accessories

UniVessel® SU Culture Vessel

Disposable pre-sterilized polycarbonate culture vessels for cell culture applications
Material Polycarbonate
Sizes [L] 2
Total volume [L] 2.6
Working volume [L] 0.6 – 2
Equipment Packages
BIOSTAT® A is available in pre-defined product packages. The standard equipment packages include
everything you need for cultivation. With the advanced package, you can conveniently control your
BIOSTAT® A using your table or smartphone and perform fed-batch processes.

BIOSTAT® A package BIOSTAT® A package

for microbial fermentation for cell culture applications
Standard equipment package Standard equipment package with UniVessel® Glass
BIOSTAT® A Control Tower incl. motor BIOSTAT® A Control Tower incl. motor
Two-gas aeration (air, O2) Four-gas aeration (air, O2, N2, CO2)
Three internal pumps, fixed speed Three internal pumps, fixed speed
Digital probes for pH and DO Digital probes for pH and DO
Foam | Level probe Foam | Level probe
Two external analog signal inputs, 4–20 mA Two external analog signal inputs, 4–20 mA
pH control, acid base pH control, acid | CO2 base
DO control DO control
Combined foam | level control Combined foam | level control
Two external analog signal inputs, 4–20 mA Two external analog signal inputs, 4–20 mA
Chiller for culture vessel and exhaust cooler Filter heater
Exhaust cooler UniVessel® Glass 1 L, 2 L or 5 L, single-walled
UniVessel® Glass 1 L, 2 L or 5 L, single-walled Motor coupling
Motor coupling Three-blade segment impeller
Six-blade disk impeller Agitator shaft
Agitator shaft Heating blanket
Cooling finger Blind plugs for all ports
Heating blanket Micro-sparger
Blind plugs for all ports Four-way addition port
Ring sparger Manual sampler
Four-way addition port Universal adapter
Manual sampler Harvest pipe, adjustable height
Baffles Inoculation port
Harvest pipe, adjustable height Air filter
Inoculation port Tool and accessory set
Air filter Laptop
Tool and accessory set
Laptop
Standard equipment package with UniVessel® SU
BIOSTAT® A Control Tower incl. motor
Four-gas aeration (air, O2, N2, CO2)
Three internal pumps, fixed speed
Two external analog signal inputs, 4–20 mA
pH control, acid | CO2 base
DO control
Filter heater
Two UniVessel® SU 2L
Heating blanket
UniVessel® SU motor adapter
UniVessel® SU holder
UniVessel® SU holder adapter ring, 2 L
Temperature sensor, Pt100
Safety valve station
Advanced equipment package Advanced equipment package
Operation using tablet or smartphone* Operation using tablet or smartphone*
Substrate controller in the operator software for fed-batch Substrate controller in the operator software for fed-batch
process management process management
External, variable speed pump for substrate External, variable speed pump for substrate

* Tablet | smartphone not included

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Sartorius
Sartorius
Stedim
Stedim
Sartorius
Stedim
Australia
Australia
Australia
StedimPty.Pty.
Australia
Ltd.
Pty.Ltd.
Ltd. Pty. Technology
Ltd. Technology
Technology Park
Technology
Park
Park
Malaysia
Malaysia
Malaysia
Park Malaysia
2092
2092 Budakeszi
Budakeszi
Post
Post Box
Box Sartorius
Sartorius Stedim Australia
StedimDriveAustralia Pty.Pty. Ltd.
BukitLtd.
Bukit
Bukit
JalilJalilBukitTechnology
Jalil Technology
Jalil Park
Park Malaysia
Malaysia
hone
Phone
e +36.23.457.227
+36.23.457.227
+36.23.457.227
Phone +36.23.457.227 01001
01001
01001
Kiev,Kiev,
Kiev,
Ukraine
01001
UkraineKiev,440
Ukraine 440 “B”
Ukraine“B” UnitUnit
Unit
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Drive 57000 Bukit
Bukit Jalil
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Phone
Phone +36.23.457.227
+36.23.457.227 01001
01001 Kiev,
Kiev, Ukraine
Ukraine Dandenong
Dandenong
Dandenong South
Dandenong
South
South
Vic 3175
Vic
South
3175
3175 Vic 3175 57000
57000
Kuala
Kuala
Kuala
57000
Lumpur,
Lumpur,
Lumpur,
Kuala
Malaysia
Malaysia
Lumpur,
MalaysiaMalaysia
+36.23.457.147
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Fax+36.23.457.147
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Dandenong South South Vic Vic 3175
3175 57000
57000 Kuala Kuala Lumpur,
Lumpur, Malaysia
Malaysia
Fax
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+36.23.457.147 Phone
Phone
Phone
+380.44.411.4918
+380.44.411.4918
+380.44.411.4918
Phone +380.44.411.4918
Phone
Phone +380.44.411.4918
+380.44.411.4918 Phone
Phone
Phone
+61.3.8762.1800
+61.3.8762.1800
+61.3.8762.1800
Phone +61.3.8762.1800 Phone
Phone
Phone
+60.3.8996.0622
+60.3.8996.0622
+60.3.8996.0622
Phone +60.3.8996.0622
FaxFax
+380.50.623.3162
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+380.50.623.3162
Fax +380.50.623.3162 Phone
Phone +61.3.8762.1800
+61.3.8762.1800 Phone
Phone +60.3.8996.0622
+60.3.8996.0622
Fax
Fax +380.50.623.3162
+380.50.623.3162 FaxFax
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Fax+61.3.8762.1828
+61.3.8762.1828
Fax +61.3.8762.1828 FaxFax
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Fax+60.3.8996.0755
+60.3.8996.0755
Fax +60.3.8996.0755
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aly Italy Fax
Fax +61.3.8762.1828
+61.3.8762.1828 Fax
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+60.3.8996.0755
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artorius
Sartorius Italy
Italy
Stedim
Stedim
Sartorius
Stedim
Italy
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S.p.A.
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S.p.A.
S.p.A.
Italy S.p.A.
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Sartorius
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dell’Antella,
dell’Antella,
Via 76/A Stedim
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76/A
76/A 76/A Italy
Italy S.p.A.
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Singapore
Singapore Singapore
0012
50012 Via
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2 Antella-Bagnodell’Antella,
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50012 Antella-Bagno
a Ripoli 76/A
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Stedim
Singapore
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Stedim Pte.
Singapore
Pte.
Ltd.
Pte.Ltd.
Ltd.Pte. Ltd.
50012
50012 Antella-Bagno
Antella-Bagno aa Ripoli Ripoli (FI)
(FI) 1 Science
1 1Science
Science
Park1Park Sartorius
Science
Park
Road,Road, ParkStedim
Sartorius
Road, Road, Singapore
Stedim Singapore Pte. Pte
hone
Phone
e +39.055.63.40.41
+39.055.63.40.41
+39.055.63.40.41
Phone +39.055.63.40.41 11 Science
Science Park
Park Road,
Road,
Publication No.: SBI1518-e160404 · Order No.: 85037-544-83 · Ver. 04 | 2016

Phone
Phone +39.055.63.40.41
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+39.055.63.40.526 Singapore
Singapore
Singapore Singapore
Science
Science
Science
Park Science
ParkII II II Park
Park II
Singapore
Singapore
Singapore 117528 Singapore
117528Singapore
Singapore
117528 117528 Science
Science Park Park IIII
Singapore
Singapore 117528 117528
Phone
Phone
Phone Phone +65.6872.3966
+65.6872.3966
+65.6872.3966
+65.6872.3966
FaxFax
+65.6778.2494
Fax+65.6778.2494
+65.6778.2494Phone
Phone +65.6872.3966
Fax +65.6778.2494 +65.6872.3966
Fax
Fax +65.6778.2494
+65.6778.2494
South
South
South
Korea South Korea
Korea
Korea
Sartorius
Sartorius
Sartorius
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Korea South
Sartorius
South
Korea
Biotech Korea
Korea
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Biotech
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Co., Biotech
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GyeongGi-Do,
PanGyoYeok-Ro
PanGyoYeok-Ro 463-400
463-400
463-400
220, 463-40
220, BunDang-
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Phone
Phone
Phone PhoneSeongNam-Si,
SeongNam-Si,
+82.31.622.5700 +82.31.622.5700
+82.31.622.5700
+82.31.622.5700 GyeongGi-Do,
GyeongGi-Do, 46 46
FaxFax Fax +82.31.622.5799
+82.31.622.5799
Fax+82.31.622.5799
+82.31.622.5799
Phone
Phone +82.31.622.5700
+82.31.622.5700
Fax
Fax +82.31.622.5799
+82.31.622.5799

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