RESPONSE OF DUNDRAFF TO DIFFERENT CONCENTRATION LEVEL OF

OKRA SHAMPOO

A Research Paper Proposal To

Anao High School Department
Anao, Tarlac

In Partial Fulfillment of the Requirements For

Practical Research 2
(Quantitative Research)
 INTRODUCTION

Dandruff is a condition affecting the scalp, wherein the old cells of the scalp are

replaced by new cells at a faster rate than normal, leading to a flaky appearance of the skin,

followed by itching. Though the exact cause of dandruff is not yet known, scientists

speculate that poor hygiene and several other factors may contribute to its development.

Other skin conditions like people with skin conditions such as eczema (a chronic,

inflammatory skin condition) or psoriasis (a skin condition marked by a rapid build-up of

rough, dry, dead skin cells that form thick scales) may appear to have dandruff. There are

different types of dandruff. On dry scalps, dandruff is made up of dry, small, fine white

flakes that easily shed and are thus visible on dark clothing. On oilier scalps, the flakes are

larger and thicker and tend to stick to the scalp at the base of the hair. Dandruff can also be

accompanied by an itchy scalp. Seborrheic dermatitis of the scalp is dandruff at its worst,

causing the skin around the hairline to become red and flaky. Dandruff is a common scalp

disorder affecting almost half of the population at the pre-pubertal age and of any gender

and ethnicity. No population in any geographical region would have passed through freely

without being affected by dandruff at some stage in their life. The word dandruff (dandruff,

dandriffe) is of Anglo-Saxon origin, a combination of ‘tan’ meaning ‘tetter’ and ‘drof’

meaning ‘dirty’. It has been well established that keratinocytes play a key role in the

expression and generation of immunological reactions during dandruff formation. The

severity of dandruff may fluctuate with season as it often worsens in winter.

Most Filipinos have dandruffs and almost everyone is asking for a cheap remedy.

Dandruff is harmless, so you can treat it at home without a prescription. Though effective

anti-dandruff agents are present, they are expensive and thus leading to the main reason
why many Filipinos were not able to afford them. There’s no other way to treat dandruff

aside from applying anti-dandruff agents. The overall goal of dandruff treatment is to

prevent and fight dandruff from growing through the scalp. After shampooing, we use to

apply conditioner for shiny hair. Since some conditioner causes dandruff, we came up with

the idea of “Homemade anti-dandruff conditioner from okra fruit”. Aside from it reduces

and prevents dandruff, it also adds shine and bounce to your hair.

Okra is one of the most therapeutic vegetables. This therapeutic vegetable is grown

all throughout the tropical and warm mild districts around the globe for its stringy fruits or

“pods”. Okra can be consumed as a vegetable. Okra aka “Lady’s finger” is in with the

Malvaceae (mallows) family and is named deductively as Abelmoschus esculentus. Okra

is very effective in treating scalp dandruffs which might occur to due to lack of proper care

of your hair. This is achieved due to its moisturizing effect on hair. Okra is beneficial in

improving the overall scalp condition and prevents dandruffs. Okra (Abelmoschus

esculentus) is rich in vitamins, minerals and various antioxidants such as catechin,

epicathechin, quercetin, procyandin, rutin. B1 (thiamne) and B2 (rivoflavin). These

antioxidants help prevent damaged cells caused by stressed and environmental factors.

There is lacking scientific reported study on these plant properties despite its wide usage

as medicinal plant. Therefore, the main objective of this study is to investigate the

underlying mechanism on the natural anti-dandruff substances effect in (Abelmoschus

esculentus).

Shampoos are cleaning formulations used for a wide range of applications,

including personal care, pet use, and carpets. Most are manufactured in roughly the same
manner. They are composed primarily of chemicals called surfactants that have the special

ability to surround oily materials on surfaces and allow them to be rinsed away by water.

Most commonly, shampoos are used for personal care, especially for washing the hair.

Most people use a shampoo to wash their hair on a regular basis; however, many of those

people aren't aware that this common hair care product may contain ingredients that could

be harming their health. For many, their choice of shampoo comes down to cost, brand,

perceived effectiveness or even fragrance.

OBJECTIVE OF THE STUDY

This study aims to investigate whether Okra (Abelsmoschus esculentus,

Malvaceae) has the activity of fighting or preventing the formation of dandruff in scalp.

Specifically, it aimed to do the following;

1. To measure the effectiveness of using okra shampoo in preventing dandruff in

scalp.

2. To determine which level of okra concentration is ideal in fighting dandruff

formation in scalp.

TIME AND PLACE OF THE STUDY

Our research will be conducted on December 15,2019 in Anao Senior High School

at San Francisco East, Anao, Tarlac.

 REVIEW OF LITERATURE

VARIETY OF OKRA

Okra Lee produces dwarf plants that average 2.5 to 3 feet with no large lateral

branches and begin fruiting earlier than other pollinated varieties. Tapered very dark green

pod are strictly pentagonal in shape and are best harvested when 4 to 5 inches long. Relative

maturity is 45 to 55 days. (CDCI, 2019)

BENEFITS OF OKRA

Okra is rich in vitamins A,C, and K, as well as minerals like magnesium and folate.

According to goohairdiaries.com,” The protein and oil contained I the seeds of okra serves

as the source of first-rate vegetable protein. It is enriched with amino acids on the likes of

tryptophan, cystine and other sulfur amino acids. “All of these nutrients work together to

create a hair cure-all that covers range concerns. It is usually used as a hair gel since it

offers great definition to curls and can offer lasting hold without crunchiness. Okra helps

with hair growth. It helps by keeping the scalp healthy since the scalp is the bedrock of

growth. (M. Wolff, 2017)

CAUSE OF DANDRUFF

The cause of dandruff is a naturally occurring microbe on the scalp called

Malassezia Globosa. It is found on everybody’s scalp, although it will only cause dandruff

in around 50% of people. Malassezia Globosa feeds on natural scalp oils called sebum, this

oil is what keeps the hair and scalp moisturized. As the sebum is broken down, it produces
a by-product called oleic acid. One in two people are sensitive to oleic acid and the scalp

responds by becoming irritated. In response to the irritation, the scalps start to become

inflamed, red and itchy. The body signals to the brain to shed skin cells faster than usual,

essentially, an attempt to shed the irritant. This shedding of skin is what causes visible

flakes to appear on the scalp, often falling off. (Procter and Gamble, 2019)
 RESEARCH METHODOLOGY

In this chapter, this will discuss the research and design method, the subject, instruments

and procedures that will be use.

RESEARCH DESIGN AND METHOD

The design was based on the purpose of this study, which is to determine the

effectiveness of Okra (Abelmoschus esculentus) as an anti-dandruff. To accomplish this,

an experimental design is to be used. A randomized test is to be done which is achieved

through culturing the causative agent of dandruff which is Malassezia globosa. The

cultured malassezia globosa will be treated with okra shampoo.

The researchers will BE going to harvest the okra and they will store it in temperature 8-

10⁰C in a plastic container. And they will perform the experiment in the University of Saint

Louis, Tuguegarao City Pharmacy Laboratory.

Identification of Malassezia on skin has been aided by the application of molecular

or DNA based techniques. These investigations show that the Malassezia species causing

most skin diseases in humans the most common cause of dandruff and Seborrhoeic

dermatitis is M. globosa. (De Angelis et al., 2007). These yeasts are naturally found on skin

surfaces of many animals including man.

The researchers will isolate and identify the fungus M. globosa, the dandruff-

causing and they will treat it with the formulated different concentrations of the okra

shampoo and the positive control and they will compare who will be the most effective.
PARTICIPANTS/SUBJECT/RESPONDENTS

The subject for this study will be the causative agent of dandruff (M.

globosa).

MATERIALS/INSTRUMENTS

Materials used in formulating okra shampoo

 The researches will use 10 young okras, 11 oz (325 ml) of water, four beakers (500

ml), slicer, strainer, stirring rods, container and lavender oil (7 drops).

 Materials in collecting the sample and preparing the cultured Malassezia globosa.

 In collecting the sample, the researchers will use sharp and sterile forceps, 70%

ethanol, dark sampling paper. In preparing the culture media the samples that will

be collected will be cultured on BAP (Blood Agar Plate), chloramphenicol, petri

dish, sterile forceps.

 Materials in testing the anti-dandruff activity of the shampoo

 The researchers will use a cultured Malassezia globosa to test the effectiveness of

okra

PROCEDURES

PROCEDURES IN MAKING THE DIFFERENT CONCENTRATIONS OF OKRA

SHAMPOO

In making different concentrations the researchers will use 50ml of the formulated

shampoo and add 50ml of water, 75ml of the formulated shampoo and add 25ml of water

and 100% concentrated of the formulated shampoo.

SAMPLE COLLECTION PROCEDURE

Sharp and sterile forceps will be used to detach the hair and scalp samples from the

infected persons (Cheesbrough, 2000). The infected region will first wash with 70%

ethanol followed by scraping with the sterile epilation forceps which was held at an angle

of 90o with the head. The specimen will be transferred into a dark sampling paper to

prevent exposure to sunlight. Each sample will be labelled with the patient’s name.

PROCEDURE IN MAKING THE CULTURE MEDIA

The samples collected will be cultured on petri dishes with agar incorporated with

chloramphenicol to rid the media of bacterial contaminants. Small amounts of the samples

collected will be introduced into Petri dishes containing the media using sterile forceps.

The Petri dishes will be then labelled and will be incubated at room temperature (25oC)

for three days and then, up to a week (Kindo et al., 2004; Cheesbrough, 2000).

How to prepare Petri Dish with Agar:

1. Measure the recommended amount of agar and distilled water in to a clean,

sterile flask or beaker

2. Using heat resistant hand protection, hold the beaker/flask over a flame and stir

the mixture gently using a sterile stir rod while heating

3. Continue boiling the mixture for about one minute, and then remove from heat

4. Place a sterile lab thermometer in the mixture and monitor until its temperature

falls to about 47 degrees (45- 50 degrees)

5. Pour melted agar in to the Petri dish to cover the bottom (about a quarter) and

replace the lid immediately

 6. Allow the agar plate to cool and set (the medium will set like gelatin at room

temperature)

7. It is ready for storage once it sets

Requirements

 Agar powder

 Lab thermometer

 Distilled water

 Glass stir rod

 Heat resistant hand protection

 Boiling mixture

 Sterile Petri dish

 Beaker/flask

How to make homemade shampoo with Okra (Abelmoschus esculentus) as an anti-

dandruff (Naturopathy at home October 2010)

1. Remove the stalk

2. Cut each okra vertically / cross sectionally into thin slices.

3. Boiled the okras and water for about 15 minutes on a medium heat. In time,

the brew should become slimmy and slightly brown

4. Cool the brew

5. Strain the brew to separate the liquid from the fiber. The liquid is the base

from your shampoo

6. Add the essential oil (lavender) 7 drops

DATA GATHERING

STATISTICAL ANALYSIS

The data collected on different growth and yield parameters will be subjected to

Analysis of Variance (ANOVA) by using Microsoft Excel Computer Software. Mean

separation will be carried out using least significant difference (LSD) at 5% probability

level statistically.