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ABSTRACT
Acne vulgaris is a disease of pilosebaceous unit which is characterized by non-inflammatory and inflammatory lesions. Common therapies that are used for the
treatment of acne include topical, systemic, hormonal, herbal and combination therapy. So, for the treatment of acne herbal anti acne cream is prepared
containing Withania somnifera extract, which is known for its anti-inflammatory action and antioxidant property. Herbal medications are considered safer than
allopathic medicines because allopathic medications are associated with different side-effects such as allergy, local irritation, scaling, photosensitivity reaction,
itching, peeling, redness etc. The study includes macroscopical and microscopical evaluation of Withania somnifera containing ash value, loss on drying,
water extractive value, Fourier Transform Infrared Spectroscopy, Differential Scanning Calorimetry and Ultraviolet Spectroscopy. The prepared formulation
was optimized on the basis of in vitro release study. The optimized formulation was evaluated on the basis of greasiness, spreadability, after feel, skin
irritancy, viscosity, pH and release kinetics. Anti bacterial study by disc diffusion technique was performed. The result obtained was zone of inhibition of test
25.66 ± 1.12 and 5.89 ± 1.18 at 500 ppm, this proved that optimized formulation has possible anti acne property. So, further investigation such as in vivo
studies will help this formulation to be a potential candidate as marketed product.
Keywords: Withania somnifera, Acne vulgaris, anti acne cream, FTIR, skin feel test.
Ingredients F1 F2 F3
Withania somnifera extract 10 % 10 % 10 %
Sulphurated potash 3% 3% 3%
Roghan Aneesi 0.04 % 0.04 % 0.04 %
Roghan laung 0.04 % 0.04 % 0.04 %
Kushta zast 3% 3% 3%
Zinc oxide 5% 5% 5%
Khushboo rose 1.25 % 1.25 % 1.25 %
Stearic acid 0.9 % 0.7 % 1.1 %
Cetyl alcohol 16.22 % 16.22 % 16.22 %
Tri ethanolamine 0.51 % 0.51 % 0.51 %
Isopropylmyristate 0.95 % 1% 0.90 %
Methyl paraben 0.095 % 0.095 % 0.095 %
Glycerine 0.9 % 1.15 % 0.65 %
Water 58 % 57.99 % 58.19 %
Evaluation of Cream
pH
pH of the formulation was found to be 6.9 ± 0.5.
Viscosity
Viscosity of the formulation was measured after 24 h of the Figure 3: DSC curve of withanolide
formulation and was found to be 33.5 ± 0.3 cps.
The melting point of extract was found to be 247.6330C
Spreadability which is found to be in accordance with the melting point of
Spreadability of the cream was found to be 40.8 ± 0.5 g/sec. withanolide i.e. 2450 C – 2490C. This shows that withanolide
is present in the extract of Withania somnifera.
After Feel Effect
Cream was found to be of emollient nature and non greasy.
The test was performed to confirm the safety of the cream. It REFERENCES
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