RESULT

Table 1: Data of initial and final reading for pH, Dissolved Oxygen, TSD, TSS AND TDS

Bottle Initial pH Final pH DO Reading (mg/L) TSD (g) TSS (g) TDS (g)
Number Initial DOI Final DOF Initial Final Initial Final Initial Final
Blank 8.67 8.02
1 6.98 6.83 6.34 4.77 50.7431 50.7460 0.2472 0.2476 43.6305 43.6331
2 7.01 6.92 6.26 4.88 47.1836 47.1841 0.2478 0.2479 49.6582 49.6583
3 6.93 6.81 6.27 5.47 50.688 50.6913 0.2459 0.2468 47.780 47.7808
4 6.85 6.79 6.34 5.26 49.4724 49.4858 50.4423 50.4433 55.0013 55.0355
5 6.96 6.82 6.26 4.80 48.5797 48.5851 55.0759 55.0780 48.5772 48.5797
6 6.91 6.92 6.32 5.20 47.15 47.1576 51.91 51.9120 49.45 49.4556

Table 2: Result of calculation for BOD, TSD, TDS and TSS test.

Bottle Number BOD Result (mg/L) TSD (mg/L) TDS (mg/L) TSS (mg/L)
Blank 0.520 - - -
1 1.256 0.0967 0.0867 0.0133
2 1.104 0.0167 0.003 0.003
3 0.64 0.11 0.0267 0.03
4 0.864 0.4467 1.14 0.03
5 1.168 0.18 0.0833 0.07
6 0.896 0.2533 0.1867 0.0667
 Graft of BOD Content for Each Bottles
1.4
1.2
BOD (mg/L) 1
0.8
0.6
0.4
0.2
0
0 1 2 3 4 5 6 7
Number of Sample (L)

Figure 1: Graft of biochemical oxygen demand for each sample.

Total Solid Dried (g) vs Number of Sample (L)

0.5

0.4
TSD (g)

0.3

0.2

0.1

0
0 1 2 3 4 5 6 7
Number of Sample (L)

Figure 2: Total solid dried for each sample.

Total Dissolved Solid (g) vs Number of Sample (L)

1.2
1
0.8
TSD (g)

0.6
0.4
0.2
0
0 1 2 3 4 5 6 7
Number of Sample (L)

Figure 3: Total dissolved solid for each sample

 Total Suspended Solids (g) vs Number of Sample
(L)
0.08
0.07
0.06
0.05
TSS (g)

0.04
0.03
0.02
0.01
0
0 1 2 3 4 5 6 7
Number of Sample (L)

Figure 4: Total suspended solid for all the sample.

APPENDICES

FIGURES

TSD Initial weight (g) TSD Final weight (g)

Figure A1: Picture of weight for Total solid dried for initial and final

TSS Initial weight (g) TSS Final weight (g)

Figure A2: Picture of weight for Total Suspended Solid for initial and final

TDS Initial weight (g) TDS Final weight (g)

Figure A3: Picture of weight for Total Dissolved Solid for initial and final
CALCULATION

1) Total Solid Dried (TSD)

(𝐀 − 𝐁) 𝐱 𝟏𝟎𝟎𝟎
𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 =
𝐬𝐚𝐦𝐩𝐥𝐞 𝐯𝐨𝐥𝐮𝐦𝐞, 𝐦𝐋

Where;

A = weight of dried residue + dish [mg],

B = weight of dish, [mg]

Bottle Number 1

(50.7460 − 50.7431) x 1000

mg total solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟗𝟔𝟕 𝐦𝐠/𝐋

Bottle Number 2

(47.1841 − 47.1836) x 1000

mg total solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟏𝟔𝟕 𝐦𝐠/𝐋

Bottle Number 3

(50.6913 − 50.688) x 1000

mg total solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟏𝟏 𝐦𝐠/𝐋

Bottle Number 4

(49.4858 − 49.4724) x 1000

mg total solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟒𝟒𝟔𝟕 𝐦𝐠/𝐋

Bottle Number 5

(48.5851 − 48.5797) x 1000

mg total solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟏𝟖 𝐦𝐠/𝐋

Bottle Number 6

(47.1576 − 47.15) x 1000

mg total solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟐𝟓𝟑𝟑 𝐦𝐠/𝐋

2) Total Dissolved Solids (TDS) Dried at 180C

(𝐀 − 𝐁) 𝐱 𝟏𝟎𝟎𝟎
𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐝𝐢𝐬𝐬𝐨𝐥𝐯𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 =
𝐬𝐚𝐦𝐩𝐥𝐞 𝐯𝐨𝐥𝐮𝐦𝐞, 𝐦𝐋

Where;

A = weight of dried residue + dish [mg],

B = weight of dish, [mg]

Bottle Number 1

(43.6331 − 43.6305) x 1000

mg total dissolved solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐝𝐢𝐬𝐬𝐨𝐥𝐯𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟖𝟔𝟕 𝐦𝐠/𝐋

Bottle Number 2

(49.6583 − 49.6582) x 1000

mg total dissolved solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐝𝐢𝐬𝐬𝐨𝐥𝐯𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟎𝟑 𝐦𝐠/𝐋

Bottle Number 3

(47.7808 − 47.780) x 1000

mg total dissolved solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐝𝐢𝐬𝐬𝐨𝐥𝐯𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟐𝟔𝟕𝐦𝐠/𝐋

Bottle Number 4

(55.0355 − 55.0013) x 1000

mg total dissolved solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐝𝐢𝐬𝐬𝐨𝐥𝐯𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟏. 𝟏𝟒 𝐦𝐠/𝐋

Bottle Number 5

(48.5797 − 48.5772) x 1000

mg total dissolved solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐝𝐢𝐬𝐬𝐨𝐥𝐯𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟖𝟑𝟑 𝐦𝐠/𝐋

Bottle Number 6

(49.4556 − 49.4500) x 1000

mg total dissolved solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐝𝐢𝐬𝐬𝐨𝐥𝐯𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟏𝟖𝟔𝟕 𝐦𝐠/𝐋

3) Total Suspended Solids (TSS) Dried at 103 – 105C

(𝐀 − 𝐁) 𝐱 𝟏𝟎𝟎𝟎
𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐮𝐬𝐩𝐞𝐧𝐝𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 =
𝐬𝐚𝐦𝐩𝐥𝐞 𝐯𝐨𝐥𝐮𝐦𝐞, 𝐦𝐋

Where;

A = weight of dried residue + dish [mg],

B = weight of dish, [mg]

Bottle Number 1

(0.2476 − 0.2472) x 1000

mg total suspended solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐮𝐬𝐩𝐞𝐧𝐝𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟏𝟑𝟑 𝐦𝐠/𝐋

Bottle Number 2

(0.2479 − 0.2478) x 1000

mg total suspended solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐮𝐬𝐩𝐞𝐧𝐝𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟎𝟑 𝐦𝐠/𝐋

Bottle Number 3

(0.2468 − 0.2459) x 1000

mg total suspended solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐮𝐬𝐩𝐞𝐧𝐝𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟑 𝐦𝐠/𝐋

Bottle Number 4

(50.4433 − 50.4423) x 1000

mg total suspended solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐮𝐬𝐩𝐞𝐧𝐝𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟑 𝐦𝐠/𝐋

Bottle Number 5

(55.0780 − 55.0759) x 1000

mg total suspended solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐮𝐬𝐩𝐞𝐧𝐝𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟕 𝐦𝐠/𝐋

Bottle Number 6

(51.9120 − 51.91) x 1000

mg total suspended solids/L =
30, mL

𝐦𝐠 𝐭𝐨𝐭𝐚𝐥 𝐬𝐮𝐬𝐩𝐞𝐧𝐝𝐞𝐝 𝐬𝐨𝐥𝐢𝐝𝐬/𝐋 = 𝟎. 𝟎𝟔𝟔𝟕 𝐦𝐠/𝐋

Determination of BOD in sample from Cherana Putih

For blank sample:

𝐁𝐥𝐚𝐧𝐤 = 𝐁𝐥𝐚𝐧𝐤 𝐃𝐎𝐢 − 𝐁𝐥𝐚𝐧𝐤 𝐃𝐎𝐟

Where DOi = initial DO of seed control, mg O2/L

DOf = final DO (after five days incubation), mg O2/L

Blank = (8.67 – 8.02) mg/L

Blank = 0.65 mg/L

For seed control samples:

𝐒𝐞𝐞𝐝 𝐟𝐚𝐜𝐭𝐨𝐫 = (𝟐/𝟏𝟎) ( 𝐒𝐞𝐞𝐝 𝐃𝐎𝐢 − 𝐒𝐞𝐞𝐝 𝐃𝐎𝐟 )

Where DOi = initial DO of seed control, mg O2/L

DOf = final DO of seed control, mg O2/L

2/10 = ratio of seed amount in sample (2ml) and seed control (10 ml)

Blank

Seed factor = (2/10) (8.67 – 8.02)

Seed factor = 0.13 mg/L

Bottle Number 1

Seed factor = (2/10) (6.34 – 4.77)

Seed factor = 0.314 mg/L

Bottle Number 2

Seed factor = (2/10) (6.26 – 4.88)

Seed factor = 0.276 mg/L

Bottle Number 3

Seed factor = (2/10) (6.27 – 5.47)

Seed factor = 0.160 mg/L

Bottle Number 4

Seed factor = (2/10) (6.34 – 5.26)

Seed factor = 0.216 mg/L

Bottle Number 5

Seed factor = (2/10) (6.26 – 4.80)

Seed factor = 0.292 mg/L

Bottle Number 6

Seed factor = (2/10) (6.32 – 5.20)

Seed factor = 0.224 mg/L

For sample

𝐁𝐎𝐃𝟓 = [(𝐒𝐚𝐦𝐩𝐥𝐞 𝐃𝐎𝐢 − 𝐒𝐚𝐦𝐩𝐥𝐞 𝐃𝐎𝐟 ) − 𝐒𝐞𝐞𝐝 𝐅𝐚𝐜𝐭𝐨𝐫 ](𝟑𝟎𝟎⁄ 𝐕)

Where DOi = initial DO, mg O2/L

DOf = final DO (after five days incubation), mg O2/L

V = sample volume, added tp 300 ml BOD bottle, ml

Blank

BOD5 = [(8.67 – 8.02) – 0.130] (300/300)

BOD5 = 0.520 mg/L

Bottle Number 1

BOD5 = [(6.34 – 4.77) – 0.314] (300/300)

BOD5 = 1.256 mg/L

Bottle Number 2

BOD5 = [(6.26 – 4.88) – 0.276] (300/300)

BOD5 = 1.104 mg/L

Bottle Number 3

BOD5 = [(6.27 – 5.47) – 0.160] (300/300)

BOD5 = 0.64 mg/L

Bottle Number 4

BOD5 = [(6.34 – 5.26) – 0.216] (300/300)

BOD5 = 0.864 mg/L

Bottle Number 5
BOD5 = [(6.26 – 4.80) – 0.292] (300/300)

BOD5 = 1.168 mg/L

Bottle Number 6

BOD5 = [(6.32 – 5.20) – 0.224] (300/300)

BOD5 = 0.896 mg/L