Beruflich Dokumente
Kultur Dokumente
Biochemistry
Class Test Question Collection
1/2019 batch
Biochemistry Eindra
1 Enzymes 2
2 Blood Biochemistry 4
3 Respiratory Biochemistry 6
7 Molecular Biology 12
8 Cell Signaling 14
9 Acid-Base Balance 16
13 Biomolecules 23
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Biochemistry Eindra
( 1/2019 ) Batch
Enzymes
UM-1
1. Define Enzyme and coenzyme and state their chemical natures. ( 5 mks )
2. What are the mechanisms that regulate the activity of the rate limiting enzyme of a
biosynthesis pathway. ( 10 mks )
3. State the mechanism of enzyme catalysis. ( 5mks )
UM-2
UMM
UMMG
2
Biochemistry Eindra
UMTG
1. List the factors that decrease the velocity of enzyme catalyzed reaction. ( 4mks )
2. Which factors cause denaturation of enzymes? How these factors cause denaturation of
enzymes ? ( Mention abt only TWO factors ) ( 11 mks )
3. Name 3 suicide inhibitors of enzymes and explain ONE. ( 9 mks )
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Biochemistry Eindra
Blood Biochemistry
UM – 1
1. State the principle of Benedict’s test and describe its clinical application. ( 5 mks )
2. A 12-yr-old boy was referred by his family physician for Jaundice and recurrent acute
bone pain. Blood film revealed normocytes and some sickle cells.
a. Explain Heme Breakdown and discuss the cause of jaundice in this patient.
( 10 mks )
b. Give the genetic and phenotypic explantion of development of sickle cell. (5 mks)
3. Explain the transport of various molecules by plasma proteins. ( 5mks )
4. Describe the role of Tissue type Plasminogen Activator in dissolving fibrin clot. ( 5mks )
UM- 2
UMM
UMMG
1. State the factors that cause changes in plasma protein concentrations. ( 7 mks )
2. Describe heme breakdown in liver. ( 6 mks )
3. What is hyperbilirubinemia and what is Jaundice ? ( 4 mks )
4. What is Van de Berg’s test? Explain its importance in differentiation of Jaundice. (
5mks )
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Biochemistry Eindra
UMTG
5
Biochemistry Eindra
Respiratory Biochemistry
UM -1
1. Draw and label the Oxygen-Hb dissociation curve. ( 4mks )
2. Explain how the Oxygen delivery form Hb to the exercising muscle is increased.
( 11mks )
UM- 2
1. State the structure of Hemoglobin A. ( 5mks )
2. Discuss the role of Hemoglobin in CO2 transport. ( 10 mks )
3. Short Note : CO poisoning ( 5 mks )
UMM
UMMG
1. Why does fetal Hemoglobin ( HbF ) have increased Oxygen affinity than maternal
hemoglobin( Hb A ) ? ( 5mks )
2. Draw the Oxygen-Hb dissociation curve and explain why it shifts to the right during
exercise. ( 10 mks )
3. Short Note : CO poisoning ( 5 mks )
4. Describe the structure and functions of Hemoglobin A1. ( 8mks )
UMTG
6
Biochemistry Eindra
UM – 2
UMM
UMMG
UMMTG
1. A 4-yr old boy was brought for consultation for hematuria, edema of lower extremities
as well as swollen right leg. He was the 12th born in a poor family, where one previous
child died from malnutrition and dehydration in the period of infancy. The child was fed
only with cow’s milk and biscuits.
From the period of five mnths, the boy manifested irritability, sweating, poor appetite
and cried when somebody touched him.
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Biochemistry Eindra
At admission the baby was afebrile, pale and malnourishe ; his hair was dry and cracked.
Clinical evaluation showed no organomegaly, no neurological signs, gingival bleeding
was there and only one tooth was present.
a. What is the probable diagnosis for this child and define this defect? ( 5mks )
b. Explain the coenzyme functions and anti-oxidant function of this defective
vitamin. ( 10 mks )
2. Name the enzyme and coenzyme/cofactor for each of the following reactions and write
down complete equation. ( 15 mks )
a. Succinate -------------- Fumarate
b. Acetyl CoA ------------- Malonyl CoA
c. Pyruvate --------------- Alanine
d. Pyruvate --------------- Acetyl CoA
e. Hydrogen peroxide --------- Water
f. Lysyl residues ------------- Allysine
g. Homocysteine ---------- Methionine
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Biochemistry Eindra
UM – 1
1. State the important functions of carbohydrate related to the body energy provision.
( 6mks )
2. List the anthropometric measures to assess obesity. Give the nutritional status of you
after calculating the BMI from your height and weight. ( 5mks )
3. Short Note : Positive energy balance. ( 5mks )
UM – 2
1. The following table shows dietary record of 19 yr old , female, 2 nd MBBS student on last
Sunday. Calculate her calorie intake and proportion of carbohydrate, fat and protein in
the diet. Does she have adequate diet? Recommend how adequate diet should be
planned. ( 15mks )
UMM
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Biochemistry Eindra
UMMG
1. How will you plan a balanced diet to maintain well-being of an individual ? ( 5mks )
2. Short Note : PEM ( 5mks )
3. Describe the role of leptin in energy regulation. ( 5mks )
4. Write short account on calorimetry. ( 5mks )
5. Short Note : Diet Induced Thermogenesis ( 5mks )
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Biochemistry Eindra
UM – 1
1. Explain why mitochondria is referred to as the power house of the cell. ( 15mks )
UM – 2
1. Outline the flow of electrons from NADH ( produced by NAD+ linked dehydrogenases )
in Electron Transport Chain ( ETC ) . ( 7mks )
2. Explain the mechanism how electron flow in ETC is linked to ATP synthesis. ( 8mks )
UMM
1. What are the energy rich compounds ? Describe the role of ATP synthase complex in
synthesis of ATP. ( 8mks )
UMMG
UMTG
11
Biochemistry Eindra
Molecular Biology
UM – 1
1. How does a cell system respond when it sense an error in DNA replication ? And explain
how the error is repaired. ( 6mks )
2. Draw a diagram of functional eukaryotic gene with necessary labeling. ( 4mks )
3. What is cell cycle ? What are the phases of cell cycle? ( 5mks )
4. Explain how a cell progresses in G1 phase and overcomes the restriction point to enter
the S phase. ( 5mks )
5. Give a short account on DNA packaging. ( 5mks )
6. List different types of RNA. State the functions of them. ( 10mks )
UM – 2
UMM
12
Biochemistry Eindra
UMMG
UMTG
13
Biochemistry Eindra
Cell Signaling
UM -1
1. Explain how growth factors signal the stem cells to initiate proliferation. ( 5mks )
2. Give an account on Guanine nucleotide binding protein and its biomedical importance.
( 8mks )
3. Explain the mechanism of how Vit D increases blood Calcium from dietary calcium.
( 7mks )
4. Explain insulin synthesis in pancreas and how it is trafficked into the blood. ( 8mks )
5. Give general description on hormone secretion in Hypothalmo-pituitary-thyroidal axis.
( 7mks )
UM – 2
UMM
UMMG
1. What are glycoprotein hormones? Describe the signaling mechanisms of TSH in thyroid
hormone synthesis and secretion. ( 10 mks )
2. What are intracellular mediators ? ( 5mks )
3. List the hormones derived from tyrosine. Describe the signaling mechanism of TSH in
thyroid hormone synthesis and secretion. ( 10mks )
4. State the biochemical significance of Receptors. ( 2mks )
5. Explain the hormonal interactions with examples. ( 5mks )
6. Short Note : Aromatase ( 5mks )
14
Biochemistry Eindra
UMTG
15
Biochemistry Eindra
Acid-Base Balance
UM – 1
UM – 2
1. Discuss the importance of bicarbonate buffer system in respiratory and renal regulation
of acid-base homeostasis. ( 15mks )
UMM
UMMG
UMTG
1. A 60 yr old man was brought to hospital in a very serious condition. The patient
complained of constant vomiting containing several hundred ml of dark brown fluid
from the previous two days plus several episodes of melaena. Past history of alcoholism,
cirrhosis, portal hypertension and a previous episode of bleeding varices were there.
Arterial Blood Gases revealed :
pH – 7.10
pCO2 – 13.8 mmHg
PO2 - 103 mmHg
HCO3 - 14.1 mmol/L
Na – 135 mmol/L
Cl - 85 mmol /L
K - 4.2 mmol/L
Glucose – 52 mmol/L
Urea – 38.6 mg/dl
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Biochemistry Eindra
17
Biochemistry Eindra
1. Describe the mechanism of iron absorption in GI tract and explain its regulation in a case
of iron overload. ( 7mks )
2. Describe the digestion of protein in GI tract. ( 8mks )
UM – 2
1. Discuss the digestion process of major foodstuffs in luminal phase of the small intestine.
( 15 mks )
UMM
UMMG
UMTG
18
Biochemistry Eindra
1. List the components of bile and state the functions of bile. ( 5mks )
2. Discuss epoxide metabolism . ( 4mks )
3. Describe the carbohydrate metabolism in liver. ( 6mks )
4. Explain the biochemistry of gall stone formation. ( 7.5mks )
UM – 2
UMM
1. A man aged 55yr presented with ascites and generalized odema. Liver function tests
were done to confirm the diagnosis. Lab results are
total plasma protein - 5.5 g/dl
Serum Albumin – 1g/dl
Serum globulin - 4.5 g/dl
Prothrombin time - 10 sec
a. Write short account on role of liver in protein metabolism . ( 5mks )
b. Interpret the lab data of this patient. ( 10mks )
UMMG
19
Biochemistry Eindra
UMTG
20
Biochemistry Eindra
UM – 2
UMM
UMMG
21
Biochemistry Eindra
UMTG
22
Biochemistry Eindra
Biomolecules
UM – 1
UM -2
UMM
UMMG
UMTG
23
Biochemistry Eindra
10. What are caveolae and lipid rafts ? State the importance of caveolae and lipid rafts.
( 5mks )
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