RECOMBINANT GROWTH HORMONE APLICATION FOR GROWTH AND

IMMUNITY IMPROVEMENT OF HUMPBACK GROUPER CULTURE

Suci Antoro1, Asmanik1 and Amran1

1
) Main Center for Mariculture, Minister of Marine and Fisheries Affairs Indonesia.
Hanura-Lampung, Indonesia 35454
suciantoro@yahoo.com

Humpback grouper Cromileptes altivelis (Valenciennes, 1828) take a long time to

grow to marketable size. In this study, 50 mg of crude recombinant Epinephelus
lanceolatus growth hormone (rElGH) was added in each kg of pellet feed and applied
as daily feed for humpback grouper culture. The objective of this study is to evaluate
the effect of rElGH aplication in accelerate their growth and immunity.

The single dose of crude rElGH in Table 1. Survival Rate (SR), initial weight (W 0),
final weight (W t), specific growth rate (SGR), feed
commercial diet was applied daily for eficiency (FE), weight gain (∆W) and relative
120 days of nursery culture. The weight gain of treatment group to control (%∆W:C)
stocking density was 233 fish per m3 in of humpback grouper juvenile after rElGH orally
fiber glass tank with continuous flowing treatment for 120 days.
water. Feed consumption and mortality
Groups SR W0 Wt SGR FE (%) ∆W %∆
was recorded daily and total body (%) (g) (g) (%/days) (g) W:K
weight sampling was recorded for every control 93.14 7.26 33.73 1.28 67.59 26.47
2 weeks. At the end of the treatment, treatment 95.29 7.26 38.56 1.39 72.25 31.3 18.25
the LD50 challenge test of patogenic
bacteria Vibrio fulnificus was conducted
to measure the immunity level of fish 45

studied. b
o
40
35
d
30
y
25
Survival rate (SR), specific growth rate
(

w g 20
(SGR), feed eficiency (FE) and finally
)

e 15
i 10
relative weight gain of the treatment g
h
5
0
group after feed rElGH was higher than t I II III IV V VII VIII IX X
weeks
control group (Table 1). Relative weight
gain of the treatment group (%∆W:C) control treatment

was 18.25% higher than control. SR of Figure 1. Growth rate of humpback grouper
rElGH orally treatment after challenge juvenile after rElGH orally treatment for 120 days.
test with LD50 of patogenic bacteria V.
fulnificus was 55% higher than control 150
which was only 30% (Figure 2). S
100
R
50
These results suggested that orally
(

treatment of the rElGH able to stimulate 0

%
0 1 2 3 4 5 6 7 8
)

growth and immunity. However, this

treatment need to be continued until
consumption size so the culture
eficiency can be calculate in order to
minimize cost and maximize profit.
days after challenge test
control treatment
Figure 2. Survival rate of humpback grouper
juvenile after LD50 of patogenic bacteria Vibrio
fulnificus challenge test