BSN – 1H 10/2/2019

Group 3

Pre-Laboratory Assignment

Give the factors affecting the enzyme activity and give the normal conditions for each factor to make
enzyme perform in its optimum activity

Factors:

1. Concentration of Enzyme
The higher the enzyme level the faster the reaction will proceed noting that the
substance is in excess.

2. Concentration of substrate

The number of enzyme present is proportional to the increase of substrate

concentration until it reaches the limit rate, after further increase in the concentration it
produces no significant change in the reaction rate.

3. pH
Each enzyme has a pH value that it works at with their optimal pH level that stabilizes at
pH 7.0 – 8.0. Changes in pH may denature the enzyme.

4. Temperature
Each enzyme is extremely sensitive to thermal changes. Hence, there are certain
temperatures at which they are more likely active. 37o C to 40o C are their optimal
temperature or normal temperature. 40o C – 50o C will let the enzyme go under
denaturation.

5. Activators
To have their optimum activity some enzymes require certain inorganic metallic cations
like Mg2+, Mn2+, Zn2+ Ca2+, Co2+, Cu2+, etc. But rarely anions are needed for enzyme activity
like Chloride Ion (Cl-) for amylase.

6. Inhibitors
Inhibitors are chemicals that block or distort the active site. Inhibitors that occupy and
compete with substrate preventing it from binding to the enzyme are called Active site –
directed or competitive inhibitors. Inhibitors that attach to other parts of the enzymes
perchance distorting its shape are called non-active site – Directed or non-competitive
inhibitors.
Members:

Coronel, Jonji Dominic

Genabe, Jane Christine
Demegillo, Julianna Emm
Difuntorum, Aemilytte
Ferolin, Jasmine
Cantuja, John Paul
Eco, Allyn Eve
Gamutin, Jhulia Anne

References:

RSC Advancing the Chemical Sciences. Retrieved from

https://www.rsc.org/Education/Teachers/Resources/cfb/enzymes.htm

A- Level Biology. (2015-2019). Factors Affecting Enzyme Activity. Retrieved from

https://alevelbiology.co.uk/notes/factors-affecting-enzyme-activity/