Beruflich Dokumente
Kultur Dokumente
de Flujo y Microscopia
Confocal
10.000 ev/segundo
Laser lines: Blue Laser (488nm), Red Laser (633nm), Violet (405nm)
Software: FACs DiVa (Acquisition dot plot, countour plot and histograms). FCS
2.0-3.0, PDF image format.
Propiedades ideales de un fluorocromo
Características Generales
3. Elevada fotoestabilidad
Granulocitos
Linfocitos
R1
Monocitos
Aplicaciones de la Citometría de Flujo:
marcaje superficial
Aplicaciones de la Citometría de Flujo:
marcaje superficial
Aplicaciones de la Citometría de Flujo:
cantidad de DNA en células
G0/G1
S
M1 G2/M
M3
M2
2n 4n
Aplicaciones de la Citometría de Flujo:
Apoptosis
Apoptóticas
tardías
Necróticas
Viables Apoptóticas
tempranas
CONFOCAL LASER SCANNING
MICROSCOPY, CLSM
Laser lines: Multi Argon Laser (458nm, 488nm, 515nm), HeNe(G) Laser (543nm), LD Laser (405nm,
635nm)
Objectives: Olympus UPLANFL 4x, Olympus UPLANFL 10x, Olympus UPLANFL 20x, Olympus UPLSAPO
40x, Olympus UPLSAPO 60x (Water immersion), Olympus UPLSAPO 100x (Oil immersion)
Software: FV10-ASW 2.0 (Acquisition, 3D preview, line analysis, etc). OIB, OIF and TIFF image format.
The image of the left shows a multinucleated osteoclast cultured on dentine, stained identically to
the osteoclast in the top image. Sytox green and Alexa Fluor-488 have closely overlapping
emission spectra (both detected in the FITC ‘green’ channel).
Image: macrophages immunostained for Rab6 (Alex Fluor-488; green). Golgi apparatus stained using
wheat germ agglutinin-633(red). Bottom right panel shows colocalisation of these fluorophores in an
overlay of these images with Nomarski.
Literatura