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O GUIDE
S E NT I A L
TH E E S
AC K E R ’ S G U I D E
BI O H English
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WORLD I O
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D I Y - B
U R N E Y IN THE
YO U R JO W L E D G M ENTS
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2 The essential Biohacker’s guide - BioHackGuide SPAC BioHackGuide - The essential Biohacker’s guide 3
THE ESSENTIAL In 2008, DIYbio.org launched as a channel of commu-
nication for DIYers who wanted to build a community
around it. In 2010, the first biohacker spaces opened
BIOHACKER’S
English
in California (BioCurious) and Nueva York (GenSpace).
1.1
Version Today DIYbio.org maintains a list of the biohacker spa-
ces around the world: 41 in North America, 28 in Eu-
GUIDE rope, 6 in Latin America, 5 in Asia, and 4 in Oceania
(consulted 05/27/2016). This shows that in eight years, This manual is released under Crea-
the movement has become a global phenomenon.
BEGIN YOUR JOURNEY IN THE DIY-BIO WORLD tive Commons copyright license type
Attribution-NonCommercial-Sha-
In 2015 several of the groups in Latin America came to- reAlike 4.0 International - CC BY-
gether with the aim of promoting the movement in this -NC-SA. This license lets others mix,
region, creating the Biohacker Spaces Network of Latin tweak and build upon this work non-
Introduction commercially, as long as credit is gi-
America SyntechBio. Among the goals of this group is
The DIY-Bio (Do-it-yourself biology) movement began as an answer to ven to the creators of the manual and
the expansion of knowledge and the creation of tools for
the necessity for to public access and the democratization of Genetic En- new creations are released under
DIY-Bio in Latin America. It has driven the creation of this
gineering. This technology is known by many names, including Molecular the same terms. Links and documen-
biohacker Guide. This manual seeks to provide simple
Biology, Biotechnology, and Synthetic Biology. Genetic Engineering is key ts referenced by this manual do not
and punctual information to enable the creation of new
in the production of biomaterials, biofuels, medicines and biosensors, etc. belong to the authors of the manual,
spaces dedicated to DIY-Bio/Biohacking, helping with the this manual simply arranges the links
(Table 1).
democratization of this technology. This document is a for easy access and use. The selec-
compendium of the experiences of the pioneering groups ted links are in English language.
The beginnings of the movement can be tracked to an early promoter of DIY-
in the implementation of these spaces in Latin America.
Bio, named Rob Carlson, who in a 2005 Wired article showed that $1,000
was all it cost to start using this technology and pointed to online resources.
4 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 5
Within the DIY-Bio we start with four types of basic activities: Microbiology,
Molecular Biology, Cell Biology and Bioinformatics (Table 2). For each one of these
types of activity you need certain resources (Table 3).
6 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 7
TABLE 3 – RESOURCES
8 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 9
This equipment is optional in microbiology, the
A simple one can be built by following this
Vortex It serves to mix small amounts of liquid evenly. good use of a Bunsen burner may be enough to
video. Or a more elaborate as in this other. This tutorial shows how to build a laminar
keep the experiments free of contamination.For
Laminar flow cabinet some experiments it may be that in addition to flow cabinet. While this tutorial shows how
to build a Glovebox.
There are several alternatives to build a being sterile they need be anaerobic, and they re-
It allows to visualized microorganisms, cells, and microscope using; paper, 3D printer, using quire a GloveBox.
Microscope some cellular structures. a Smartphone or purchasing one that can be
adapted to a Smartphone.
We find this kit and this list of pH-meters at
Used to measure the pH. It is important in the reasonable prices. This equipment can also
One can be built using this tutorial. You can pH-meter preparation of culture media and solutions. be built, following this guide or any of these
It serves to stir solutions allowing them to be ho-
Magnetic stirrer choose a version with temperature control, tutorials: tutorial_1, tutorial_2.
mogeneous.
as in this tutorial.
10 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 11
Molecular Biology
Equipment Description DIY-Bio alternative
Cell biology (with animal or plant cells)
There are several low-cost models Equipment Description DIY-Bio alternative
such as this or this. There are also
This machine makes copies of DNA. It is also used to iden- It is essential for cell culture because these cultures are highly
tutorials to build one like this, which
Thermal cycler tify if a sample has a specific region of DNA using specific Laminar flow susceptible to contamination by fungi from the environment. It This tutorial shows how to build
was developed by our group in col-
primers. cabinet is also necessary to have what was already described for mi- one.
laboration with the Lab de Garagem
crobiology.
in Brazil.
Used in almost every experiment, from extraction of DNA to Incubator with For animal tissues it may be necessary to maintain certain con-
Centrifuge working with proteins. In general, a centrifuge that reaches It can be constructed by following CO2 and CO2
ditions of temperature and concentration of CO2.
11,000rpms is ideal. It is use to quickly separate different this tutorial or this. tanks
components of a solution based on their densities.
These tutorials: tutorial_1 and tuto- Consult this book: Laboratory
Useful for enzymatic reactions and transformation of cells Materials of It varies depending on the method of extraction to be used:
Water heater rial_2, explain how to build it. It can Handbook for the Fractionation
by heat-shock.
also be replaced by a thermal cycler. glass for distilla- fractional distillation, extraction by reflux, percolation, extraction of Natural Extracts by Peter J.
tion Soxhlet, etc.
Necessary for all molecular biology experiments (2uL, Houghton and Raman Amala.
Micropipettes and 20uL, 200uL and 1000uL). Used to measure and move They can be purchased or built by
disposable tips using this tutorial or this tutorial.
small volumes of liquids. Rotary evapo- It uses low pressure and temperature to separate the molecules
Used to separate DNA, RNA and proteins by size and rator extracted using solvents that are volatile.
There are tutorials such as: tu-
Electrophoresis mass, so they can be visualized later. There are vertical
torial_1, tutorial_2 and tutorial_3 There are different methods such as; thin layer chromatogra-
chamber (for DNA and RNA) and horizontal chambers (usually pro-
that explain how to build one. phy (TLC), liquid chromatography (HPLC), gas chromatography
teins). Chromatography (GC-MS), among others. While some require only a container Here see how to build DIY chro-
Used to view electrophoresis gels. It uses UV light. Handle This tutorial explains how to build equipment matography equipment.
of glass and solvents (TLC) and others use specialized equip-
Transilluminator with care. one. ment (HPLC, GC-MS).
This tutorial shows how to build
Used to break the cell wall allowing the extraction of some
Ultrasonic tank one. You can also see this other tu- There are systems to grow plants under relatively controlled conditions that can be built like this.
molecules of the cells.
torial. Other items Others are already creating robots to help grow their plants or conduct automated laboratory proto-
Used for enzymatic reactions and other experiments (0.2ml and 1.5ml) cols. Still others are already building bioprinters.
Microcentrifuge
tubes (Brand name: Eppendorf, there are cheaper options on the market)
12 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 13
Bioinformatics
Equipment Description
Preferably with an emulator of Terminal or an operating system or virtual machine
Computers and Internet access with Linux or MacOs. -mFold by Michael Zuker - Predicted secondary structures of RNA and DNA using Tm and free energy
RNA calculations.
Storage in the cloud Dropbox, Google Drive, Amazon server, etc.
The majority of bioinformatics tools can be used on-line, others are for download, -DeepView by GlaxoSmithKline & Swiss Institute of Bioinformatics - Protein structures display.
Software usually free (Mega, PDB Viewer, etc.). -Molecular Flipbook by Andrej Sali - Used for visualization in 3D of protein structures.
-VMD/NAMD by James C. Phillips et al. - Molecular display of protein structures. Molecular dynamics
*For people that is going to start working in this area we recommend reading this publication, which was developed Simulator.
Proteins
by the Leukippos Institute in collaboration with our group -ExPASy Proteomics server by the Swiss Institute of Bioinformatics - Links to calculate protein param-
eters.
-Modeller by Sali Lab - 3D modeler using homology.
14 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 15
C- Chemical and biological substances
Microbiology It is used to conserve strains of micro-
organisms for long periods of freezing You can get it in stores as Glycerin. Before use
Substance Description Formulation (-80°C). Normally, a stock of 50% is used you must sterilize the glycerol using a filter as
Glycerol
It is used to isolate and grow bacteria. You The preparation of the LB medium can be to conserve the microorganisms at a 25% explained in this video.
can consult this website for more information seen in this video, an alternative to the LB of glycerol.
LB medium
about its composition. medium can be consulted here.
You can get in stores of chemical reagents. Sub-
It is used to isolate and grow yeast. You can It is used to acidify the culture media, when
The preparation of a homemade medium Acid solution sequently the 1N solution must be prepared as
PDA culture medium consult this website, for more information it is required to lower the pH. It is usually
PDA can be seen in this video. (HCL) explained in this video, save the solution proper-
about its composition. prepare a stock solution at 1N.
ly tagged in amber glass jars.
16 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 17
Molecular Biology They are used to enable the flow of electric
It can be prepared in the biohacker space follow-
current in the electrophoresis runs and it is
TAE or TBE ing the instructions on this page for the TAE buffer
Substance Description Formulation used in the preparation of electrophoresis
buffer and this for the TBE buffer. Also, there are alternatives
gels. It is usually a prepared stock solution of
Used to cut the DNA at specific sites. You to these two buffers as described in this article.
The restriction enzymes with best ratio cost/ 50X that is used at a 1X final solution.
can check this video for more information and
functionality are those described in the stan-
this website to optimize your reaction. Also
Restriction enzymes dard of Biobricks Assembly: EcoRI, XbaI, SpeI, The agarose is a polymer extracted from al- Agarose remains a powder until used to prepare the
check this website and this website to trouble-
PstI and NotI. There are also useful: BamHI, gae that has the capability of gelling aqueous gel of electrophoresis, as this video shows. To learn
shoot the main problems if your reaction is not Agarose
BglII, XhoI, NheI and EcoRV. solutions. It is commonly used to prepare more about its preparation check this page and in this
working.
electrophoresis gels. other.
It is necessarily to buy it. Another option for
Used to identify fragments of DNA, RNA, or
DNA is to prepare a reaction of digestion with a
Molecular weight complete proteins by size or molecular mass Acrylamide solutions should be prepared with extreme
sequence of known DNA that produces a pat- Polyacrylamide is the result of a chemical
markers using electrophoresis gels. For more informa- caution. The resulting solution must be handled with
tern with different sizes of fragments and use it reaction resulting in the polymerization of its
tion you can refer to this website. precaution. To prepare a polyacrylamide gel follow the
as a marker. Polyacrylamide components, generating a gel that unlike the steps in this video. You can also check this page to
agarose gel is not affected by the temperature
learn more about SDS-PAGE, or watch this video and
They are used in cloning and expression, both and with smaller pore size than the agarose.
They can be donated by a laboratory of mo- this other.
bacteria and plants. This page is a reposito-
lecular biology or purchased and subsequently
Plasmids ry of plasmids where you can get more infor-
produced and purified in the biohacker space.
mation and order them. You can also check This buffer is used to run the polyacrylamide
As explained in this video or this other.
this video to learn more. SDS-PAGE gel in the electrophoresis chamber. Prepare
It can be prepared as it is explained on this protocol.
running buffer a stock solution at 10X, which can be used
at 1X.
It is used in molecular biology experiments. It
It can be produced in the biohacker space by
is purified and deionized water. It does not
Water passing distilled water through various filtration
contain salts, microorganisms, substances Loading This buffer serves to linearize the protein It can be prepared following this protocol. The steps
(ultrapure MilliQ) systems: reverse osmosis, UV light and filter
that may interfere with experiments. You can SDS-PAGE samples to be loaded on the electrophoresis can also be watched on this video. Consider prepar-
(20 µm).
consult this site for more information. buffer gels. ing several vials of this buffer.
18 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 19
One of the dyes used to stain proteins is The dye for proteins LAWSONA can be extract-
Tetramethylethylenediamine (TEMED). It is Coomassie blue. However there are more ed from the leaves of Henna following this proto-
To do polyacrylamide gels check this protocol for DNA Stains for proteins rapid and economic alternatives that may be col. There are dyes such as red #3 (Azorubina)
TEMED used to polymerize the polyacrylamide gels.
gels or this other for protein electrophoresis. performed in a biohacker space. that can also be used following this protocol.
Used with ammonium persulfate.
Enzyme Taq This enzyme produces copies of DNA during
It can be produced following this protocol.
polymerase the PCR, it is resistant to high temperatures.
Dodecyl sodium sulphate (SDS). Breaks Ethylene diamine tetraacetic acid. Chelating agent that catches minerals as the Mg2+ to avoid
EDTA that some enzymes from degradation of DNA or RNA.
non-covalent links from proteins, which is It can be consulted here to see the preparation of
SDS needed to perform electrophoresis. It is slight- SDS-PAGE gels.
ly irritating. It is used to join DNA fragments, usually to join DNA fragments that want to be cloned in plas-
Enzyme ligase mids that have been cut with restriction enzymes.
Nucleotides (ATCG) that constitute DNA, they are used for the synthesis of copies of DNA
Trisaminometano and hydrochloric acid. Acid dNTP’s during PCR.
It is prepared from pure HCl by diluting it with distilled
TRIS-HCl buffer that is used to lower the pH of a solu-
water and TRIS.
tion.
Reagents for It is used for the purification of plasmids.
Miniprep
Trisaminometano and sodium hydroxide. Al-
It is prepared from NaOH powder, diluted in distilled
TRIS-NaOH kaline buffer that is used to increase the pH
water and TRIS. Tubes with resin Tubes covered in treated silica so nucleic acids are trapped in their walls while the other cellular
of a solution. of silica to purify components pass through these.
nucleotides
Methyl Green is a dye that has been widely used in
Usually commonly used dyes to see the DNA histology for staining of tissues, but it is also an excel-
Stains for DNA after electrophoresis. However, there are low lent alternative for double stranded DNA as you can Chloroform, acetic acid, calcium alginate, albumin (used to stabilize enzymes/proteins solu-
Other tions).
cost alternatives that can be implemented. see in this paper. Also, you can use the blue of meth-
ylene or the violet of gentian, following this protocol.
20 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 21
Cell biology (using plant cells) Cell biology (using animal cells)
Substance Description Formulation
Substance Description
Medium of Murashige and Skoog. It contains the basic There are recipes on the internet,
DMEM medium Basic medium for cell culture.
MS medium nutrients for plant tissue cultures, the recipes may vary many modifications possible for best
depending on the type of plant. results.
Trypsin-EDTA It is used to lift the cells (which grow adhering on the walls of the flask).
Anthocyanins, cytokinins, gibberellins, ethylene, Abscisic Extracts from plants such as banana
Plant growth It maintains pH 7.2.
acid, etc. They promote the growth and/or differentiation or coconut are rich in minerals and Phosphate buffer
regulators of cells (complete plant or specific organs) plant growth regulators.
Fetal bovine serum Culture medium for animal cells.
Used to avoid contamination by bacteria and fungi/ Some plant extracts possess antibac- Antibiotics Avoid contamination of bacteria and fungi/yeasts.
Antibiotics yeasts. terial and/or antifungal properties.
22 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 23
BIOSAFETYAND
BIOHACKER SPACES REGULATION
THE DIY-BIO COMMUNITY CONTINUES TO GROW, THANKS
TO THE DEMOCRATIZATION OF TECHNOLOGY AND THE FREE
ACCESS TO THE SCIENTIFIC LITERATURE. This manual is focused on the use of beneficial organisms,
nothing mentioned in this document can or should be used
to work with other types of organisms that could represent
biohazards, have toxins or be pathogens. We believe that
More people are joining this movement, which is already getting positive results in the good practices regarding biosafety will help to a better
different countries. However, the Latin American community is still young. Therefore, diffusion of the biohackers work and towards the demo-
certain guides to operate out of an academic or business environment, and also to cratization of science. In this section we will address good
avoid to produce fear in the population are extremely valuable. The DIY-Bio community practices within a biohacker space, codes of ethics of the
philosophy includes making sure to not develop anything that could cause damage. For worldwide DIY-Bio community, international and local regu-
this reason, one of the goals of this guide is to provide basic information regarding re- lations, and regulatory agencies in Brazil, Mexico and Peru.
gulations and biosafety in the Latin American region.
24 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 25
A- LIST OF GOOD PRACTICES B- CODE OF
FOR WORKING IN ETHICS WE COMPILE A UNIVERSAL CODE OF ETHICS
AND/OR CONDUCT FOR BIOHACKERS,
A BIOHACKER SPACE OR CONDUCT USING AS BASIS THE CODES USED
BY THE COMMUNITY OF NORTH AMERICA
-Have the appropriate facilities, these faci- -Do not use your mouth to operate gra- AND OF EUROPE LISTED IN DIYBIO.ORG
lities depend on the activity and regulation duated pipettes.
of each country. -Do not eat, drink or smoke in the labora-
-Dispose the materials and reagents used tory area, and avoid the use of products
according to its nature and biosafety regu- for the skin (make-up, sunscreen, etc.) -Transparency: Promote the transparency -Peaceful purposes: Biotechnology should
lations of each country. during the activities. and share ideas, knowledge, data and re- only be used for peaceful purposes.
-Have an internal Biosafety Committee. -Wash hands before leaving the labora- sults. -Respect: Respect humans and all living
-The Biosafety Committee must train new tory. -Security: Adopt safe practices. systems.
members of the group in the proper use of -Use clothing and equipment for personal -Democratization: Promote citizen science -Responsibility: Acknowledge the complexity
equipment and experiments. protection (gloves, apron, mask, etc.) in and decentralize access to biotechnology. and dynamics of living systems and our res-
-Mark all substances and solutions indica- accordance with the activity to be carried -Education: Help to educate the public about ponsibility towards them.
ting its biological or chemical risk. Always out in the laboratory. biotechnology, its benefits and its implica- -Accountability: Be accountable for your ac-
add to the label the date of expiration, and -Store the reagents according to their che- tions. tions and for the defense of this code.
who made it. mical characteristics by isolating the ha- -Humility: Recognize that you do not know -Environment: Respect the environment.
-Consult and comply with the biological or zardous materials. everything. -Experiment: Experimenting with biology
chemical risk of substances that are to be -Avoid touching the face, eyes, mouth or -Community: Listen carefully to any concer- leads to discovery, discover leads to innova-
used. hair to avoid contamination. ns and question and answer honestly. tion.
-Flammable or toxic products must be -For more information see this Manual.
worked on, in the chemical hood.
26 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 27
C- INTERNATIONAL D- NATIONAL
REGULATIONS REGULATIONS
The most important protocols and conventions
at the international level are:
28 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 29
-Which areas will be certified? Any experiment involving genetically mo-
I. BRAZIL
-Do you have containment equipment? dified organisms can´t be done without
Profile of regulation in Brazil -Do you have equipment for the prevention having a project approved by the CIBIO.
and management of accidents?, such as
showers to wash body and eyes.
The national technical Commission of biosecurity -Which types of GMOs will be used One of the key points in the spaces is proper
-The construction.
(CTNBio) is the entity responsible for regulating and the risk classification of these? waste disposal. This disposal is regulated by
-The culture.
labor in connection with GMO (genetically modified -What level of risk the lab will have the standard PNRS (National Solid Waste Po-
-Handling.
organisms). This Commission issues The Quality (I, II, III or IV)? licy), which explains the characteristics of the-
-Transport or transfer.
Certificate in Biosecurity (CQB) that regulates -Does the laboratory meets the minimum se waste and which treatments or procedures
-The import or export.
institutions (public or private) wishing to requirements listed by the CTNBio? for disposal must be done.
-Storage, release into
conduct the following activities using GMOs: -Who will be the responsible technician
the environment or disposal.
for the laboratory?
32 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 33
A. COMMUNITY
HOW TO OPEN A
Biohackers: People who want to share information
freely about the biological sciences. To start a biohacker
movement in your town or country, you must work to
BIOHACKER
English
form a critical mass. This minimum group of people
1.1 will generate an effect on more people and create
s pace
Version
a community. This interaction should be
both virtual and face-to-face.
The community can have a leader or a support In the field of Biohacking, there is the Ne-
team, which will facilitate the organization of the twork of Biohacker Spaces of Latin Ame-
BIOHACKER SPACES NEED SOME BASIC ELEMENTS proposed activities (either by the community or rica - SyntechBio, which also has repre-
the leadership team) and the implementation of sentatives in Argentina, Brazil, Chile,
FOR ALLOWING THE FREE EXPLORATION
them in the best way. It is also Colombia, Mexico, and
OF THE BIOLOGICAL SCIENCES. important to seek alliances Peru. This network se-
with organizations that pro- eks to inspire and help
We will describe five basic elements that are common mote groups or communities. to create an ecosystem
to all biohacker spaces: In Latin America, one of these of Biohacking and Syn-
A. COMMUNITY organizations is the Associa- thetic Biology in Latin
tion of entrepreneurs of Latin America. One of the pro-
B. SPACE
America, which has repre- jects of the network is
C. EQUIPMENT AND INSTRUMENTATION sentatives in the countries of Argentina, Chile, this manual. This document aims to help
D. REAGENTS Colombia, Mexico and Peru. They promote the to open new spaces in the region, and
entrepreneurship in the region and support to the further democratize science in a more
E. FINANCING RESOURCES
new actors of this ecosystem. open way to society.
34 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 35
B. SPACE C. EQUIPMENT AND INSTRUMENTATION
Place where the biohackers gather to share Usually the biohacker spaces do not have top line
information and build projects, may be public laboratory equipment, this must be compensated
spaces or private spaces. The laboratories for, with creativity. You can build the necessary
set-up will depend on the resources of the group. equipment, find this information in the Table3: Re-
source (Chapter 1). Another option is get the equi-
pment through donations. This can be done directly
from universities and research centers, or indirectly,
A strategy to carry out this idea, is presen- Another strategy is using a space of one of the through platforms on the web as Seeding Labs.
ting the proposal for the biohacker space to a members of the group. Biohacker spaces recog-
College, a University, a Research Center, an nized at the international level have started in
incubator for business, among others. The a garage, attic or kitchen, among others. The
advantage is that you won’t need to rent a selection of these areas depends on whether
space and you may to obtain co-financing they are public or private, and to how many peo- D. REAGENTS
from the organization who welcome the pro- ple it is intended to accommodate. Freedom of
ject. In addition, professionals of the entity decisions and actions is the main advantage of
that houses the lab can help more frequent- this option. The disadvantage is that it becomes The reagents are used, along with equipment, to carry out
ly in the activities proposed. The disadvan- more difficult to raise money for activities or pro- experiments and/or reproduce existing protocols, within the
tage is that the space shall be subjected to jects, which in the majority of cases comes from biohacker space. Before purchasing any type of reagent,
the policies of the host organization, which personal funds, family and friends. However, you must assess the following points:
is not always a good thing for the biohacker there are other ways to get financing and resour-
space. ces, this is discussed in the rest of this chapter. -Corresponding biosafety local and international
regulations (Chapter 2).
-Budget of the project or space, to be
assigned to the reagents.
-Suppliers.
36 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 37
KICKSTARTER -It is a platform that accepts any type of project such as art, accessories,
E. FINANCING RESOURCES events and spaces, ideas and experiences that are new.
Projects cannot be used to raise funds for charity.
Economic resources are an important aspect to keep in mind for both -When a project involves -Create a project requires -Prices: The fees are only
the projects and the space. Each space should consider a business the manufacture and that the entity or organization charged if you arrive to the
model that allows its sustainability. It is important to find support for distribution of something is registered in the country total targeted amount. Kick-
this stage. Advice can be taken from those in the area of business or complex, such as a device, where the project will take starter takes 5% and the pay-
business incubators. the creator must show a place. The responsibility of ment institution: 3% + $0.20
prototype to the sponsors. finishing a project is exclusi- per contribution. Less than $10
There are different strategies to finance the biohacker spaces, which Is not allowed the repre- ve of the creator of the pro- contributions incur a Special
range from private to collective financing systems or better known as sentations in rendering of ject. Kickstarter does not hold Commission by “micro-contribu-
Crowdfunding. Next, we will share some of the most popular options realism photo. funds on behalf of the crea- tion” of 5% + $0.05 per contribu-
of financing. tor, does not offer refunds. tion. This for the USA.
-Two types of financing. Fixed financing: If you fail the targeted amount of money There are other platforms that can interest the reader, depending on the needs
donations are returned. Flexible financing: It does not require a fixed amount of and/or countries where the space is located: Capitall Cell, Futsci, Sciencestarter,
money to be achieved, if you fail to get all the amount the money is not returned. Fundly, Rocket Hub, Endeavorist, among others.
38 The essential Biohacker’s guide - BioHackGuide BioHackGuide - The essential Biohacker’s guide 39
II. COMPETENCES AND III. COMPETENCIES AND
INTERNATIONAL FUNDS NATIONAL FUNDS
A L
-Hello Tomorrow.
I
BRAZIL
NT
-Get it the ring.
S E
-500 StartUps. -BioMinas.
E E S
-StartUp Chile.
TH
-Seed Starts. MEXICO:
R ’ S
-StartUp Battlefield.
E
-StartUp México.
C K
-Pitch Competition. -Premio Nacional del Emprendedor.
H A
-Premio Santander.
BIO
-Premio de Innovación UNAM.
PERU:
English
1.s1 GUIDE
-StartUp Perú.
-Ideas Audaces.
IV. PRODUCTS AND SERVICES Verion
D THE SPACE CAN OFFER
-Training.
-Workshops.
-Broadcast events of science.
-Basic Kits for science activities.
-Coworking space.
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