S HM525 NX-e.SA - SA.16330.2

Cryostat HM525 NX
Cryostat
Service – Manual – English
Cat. No. 391471 – Issue 2.0 / 2013
Service Manual Cryostat HM525 NX Page 1

Thermo Fisher Scientific
Cat.No. 391471 Issue 2.0 – 2013
Company Information
© Copyright 2013. Thermo Fisher Scientific Inc. All rights reserved.
Thermo Fisher Scientific Inc. (Thermo Fisher Scientific) is the world leader in serving science, offering a unique
combination of innovative technologies. Thermo Scientific is a brand name of Thermo Fisher Scientific.
All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries.
Thermo Fisher Scientific makes every attempt to ensure that the information contained in this supporting document
is correct and clearly stated, but does not accept responsibility for any errors or omissions. The development of
Thermo Scientific products and services is an ongoing process. Please ensure that any published information you use
as a reference is up to date and relates to the condition of the product. If necessary, check with your local Thermo
Fisher Scientific representative.
This document may not, in whole or in part, be copied, photocopied, reproduced, translated, or converted to any
electronic or other form without prior written consent of Thermo Fisher Scientific. All information contained in this
manual is proprietary and confidential, and the exclusive property of Thermo Fisher Scientific and is protected by
copyright.
Contact Addresses:
Microm International, GmbH
Subsidiary of Thermo Fisher Scientific
Otto-Hahn-Strasse 1a
69190 Walldorf

Germany
USA Distributor:
Richard-Allan Scientific
Subsidiary of Thermo Fisher Scientific
4481 Campus drive
Kalamazoo
MI 49008
USA
This instrument conforms to the essential requirements of:

IVD Directive 98/79/EC

Cat.No. 391471 Issue 2.0 – 2013
Important Note Regarding Instrument Variants
This document is designed for use with the Thermo Scientific HM525 NX cryostat.
Only two variants UVC and none UVC are available on this instrument and the instructions
contained in this manual are not specific to any one variant. Thus some features described
may not be available on your instrument.

Cat.No. 391471 Issue 2.0 – 2013
Welcome
This Service Manual is a guide for troubleshooting, field repair and maintenance of the
Cryostat HM525 NX.
This Service Manual should be used along with the User Guide (cat. no. 388159) of the
Cryostat HM525 NX.
It is important that the service technician reads and understands the User Guide before using
this Service Manual.
Errors and omissions excepted. Subject to amendment.
English (printed version): 388159
Print Date: 19/08/2013
Note
This Thermo Scientific product is covered by a guarantee against defects in material or
workmanship. Any parts which may fail during the guarantee period will be repaired or replaced
free of charge. No further liability shall be accepted. Unauthorized repair or alterations by third
parties shall void the guarantee.

Cat.No. 391471 Issue 2.0 – 2013
Table of Contents
1 Safety Information 7
1-1 Symbols 7
1-2 Safety Information 8
1-3 General Safety 8
1-4 Chemical Safety 9
1-5 Environment 9
1-6 Warranty Statement 10
2 Introduction to the CryoStat HM525 NX 11
2-1 Intended Use 11
2-2 Instrument Features 11
3 Technical Specifications 12
3-1 Mechanical Specifications 12
3-2 Electrical Specifications 12
3-3 Cooling Specifications 12
3-4 Environmental Specifications 12
3-5 Microtome and Sectioning Specifications 13
3-6 Default Settings 14
4 Maintenance Methods 15
4-1 Dismantling the Casing 16
4-1-1 Remove the Rear Panel 16
4-1-2 Open the Top Cover 17
4-1-3 Remove the Handwheel 18
4-1-4 Remove the middle Part 19
4-1-5 Remove the front panel 20
4-1-6 Remove the Foot panel 21
4-1-7 Replacement of Electronic Components 22
4-2 Condensing Unit 24
4-2-1 Replace the complete Condensing Unit 24
4-2-2 Replace the Cooling fan 27
4-2-3 Replace the Starting Relay 28
4-2-3-1 Wiring of the Compressor 29
4-3 Heated Window 30
4-4 Evaporator Heater Mat & Sensor 31

Cat.No. 391471 Issue 2.0 – 2013
4-5 Electronic Control Unit (ECU) 33
4-6 C-Touch Panel 34
4-7 Cyrobar Peltier, Blade Holder and Chamber Temp Sensor 35
4-8 Microtome 36
4-9 Installation and Removal of the Cross Roller Bearings 37
4-10 Installation and Removal of the Spindle, Cylinder and Motor 39
4-11 Installation and Removal of the Link Block 41
4-12 Microtome Home Position Switch Adjustment 42
4-13 UV-Light (optional) 43
5 Analysis and Troubleshooting 44
5-1 Enter the Service Menu 44
5-2 Overview of the Service Menu 46
5-2-1 Selftest Final Check 46
5-2-2 Maintenance 51
5-2-3 Inputs 56
5-2-4 Outputs 57
5-2-5 Operating Hours 58
5-2-6 Software Update 60
5-2-7 Update C-Touch Software 61
5-2-8 Update C-ECU Software 63
5-2-9 Factory Settings 64
5-2-11 Reset Errors 67
5-2-12 Error Codes 68
5-2-13 Troubleshooting Trees 69
6 Service Assemblies and Spare Parts 78
Appendix A – Cryostat HM525 NX Service Levels 80
Appendix B – PM Procedure 81
Appendix C – Instrument and Service Module Schematics 82
Appendix C-2 – C-ECU 83
Appendix C-3 – Microtome and Motor 84
Appendix C-4 – Condensing Unit 85
Appendix D – Wiring Diagram 86
Appendix E – Schematic of the Refrigerating System 87

Cat.No. 391471 Issue 2.0 – 2013
1 Safety Information
1-1 Symbols
The following symbols and conventions may be used throughout this document and on the
instrument:
This symbol is used on the equipment, or in a document, to indicate that

instructions must be followed for safe and correct operation. If this symbol

appears on the instrument, always refer to the operator guide.

there may be a biohazard associated with the instrument. Always act with

common sense and be aware of the samples used. Take suitable precautions.
This symbol indicates that a surface is hot. If this symbol appears on the

instrument, always refer to the operator guide. Take suitable precautions.
This symbol indicates that a surface is cold. If this symbol appears on the
instrument, always refer to the operator guide. Take suitable precautions.

This symbol is show in the manual and on the instrument to indicate that

hand injuries are possible due to moving parts. Take suitable precautions.
This symbol is shown in the manual to indicate the risk of injury caused by
sharp microtome blades and knifes. Take suitable precautions.


harmful chemicals are used. Refer to the Material Safety Data Sheets for the
chemicals used. Always act with common sense and be aware of local

laboratory procedures. Take suitable precautions.
Note
Notes give more information about a job or instruction but do not form part of the instructions.
A WARNING IS GIVEN IN THE DOCUMENT IF THERE IS A

DANGER OF PERSONAL INJURY OR DAMAGE TO SAMPLES OR
EQUIPMENT.

Cat.No. 391471 Issue 2.0 – 2013
1-2 Safety Information
Thermo Fisher Scientific instruments are designed for convenient and reliable service; however, improper use or
handling by a user may damage the instrument, or cause a hazard to health. The instrument must not be used in a
manner not specified by Thermo Fisher Scientific. Correct maintenance procedures are essential for consistent
performance. It is recommended that users secure a maintenance contract with our service department.
Any problems and queries should be referred to your Thermo Fisher Scientific service department.
The following sections contain important information for the safe setup and use of the instrument,
and should be read and understood by the user before using the instrument.
1-3 General Safety

This instrument, as supplied, conforms to IEC 61010-1 and IEC 61010-2-101; however, the
addition of chemicals introduces potential hazards. Good Laboratory Practice must be employed

and consideration must be given to the potential for hazard when dealing with these chemicals.
Do not use the instrument in close proximity to strong electromagnetic radiation, as these may
interfere with the proper operation. The electromagnetic environment should be evaluated prior to

operation of the device.
Good Laboratory Practice must be used when handling tissue samples to prevent cross
contamination and infection. The user should complete a risk assessment to determine any potential
hazards related to tissue handling.
 Do not introduce any source of ignition into, or near, the instrument once it has been

loaded with reagents.
 Do not remove any panels or access covers, unless specifically instructed to do so. The
instrument does not have any user serviceable parts. Potentially lethal voltages are present
inside the instrument.
 The instrument must be properly connected to a good earth (ground) via the Mains input
supply and positioned such that it is possible to interrupt the Mains supply at the source by
removing the plug from the socket.
 Use only factory approved accessories or replacement parts within the instrument.
 Only use reagents recommended in the operator guide.

Cat.No. 391471 Issue 2.0 – 2013
1-4 Chemical Safety
The introduction of chemicals creates potential hazards. Thermo Fisher Scientific has adopted the following position
with regard to the subject of volatile chemicals used in laboratories:
 Customers using non-specified chemicals in the instrument, do so at their own risk.
 All chemicals recommended by Thermo Fisher Scientific have auto-ignition temperatures

considerably above any surface temperatures that can be reached during a single fault failure on
the instrument.
 The instrument contains no source of ignition in any areas of the instrument where chemicals are
stored, or likely to leak into, in a single fault condition.
 The operator is fully aware of the contents of the specification documents detailing the properties
of the chemicals they are using.
 The operator has carried out any legally required assessment of chemicals used and is using Good
Laboratory Practice.
 Some chemicals which may be used during operation are flammable - do not sue sources of
ignition in the vicinity of the instrument when it is loaded with reagents.
 Harmful chemical vapours such as Xylene or Toluene may be emitted during the normal
operation of some instruments and the operator should be aware of suitable precautions and

safety measures.
1-5 Environment
This product is required to comply with the European Union's Waste Electrical and
Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following
symbol:
Thermo Fisher Scientific has contracted with one or more recycling / disposal companies in
each EU Member State, and this product should be disposed of or recycled through them.
Further information on Thermo Fisher Scientific’s compliance with these Directives, the
recyclers in your country, and information on Thermo Fisher Scientific products which may
assist the detection of substances subject to the RoHS Directive are available at:
www.thermo.com/WEEERoHS

Cat.No. 391471 Issue 2.0 – 2013
1-6 Warranty Statement
We at Thermo Fisher Scientific are proud of our quality, reliability and of our after-sales
service. We continuously strive to improve our service to our customers.
Please ask you distributor or Thermo Fisher Scientific representative about Service Contracts
which can keep your purchase in peak condition for many years to come.
Warranty provisions necessarily vary to comply with differences in national and regional
legislation. Specific details can be found in the delivery documentation or from your dealer
or representative.
Please note that your warranty may be invalidated if:
 This instrument is modified in any way.

 Accessories and reagents which have not been approved by Thermo Fisher Scientific are used.
 The instrument is not operated or maintained in accordance with the instructions in the Operator
Guide.

Cat.No. 391471 Issue 2.0 – 2013
2 Introduction
Introduction to the Cryostat HM525 NX

2-1 Intended Use
The Thermo Scientific HM525 NX is a high-performance cryostat designed to prepare tissue
sections of frozen samples in medical, pharmaceutical and research laboratories. Only trained
laboratory personnel may operate the HM525 NX.
The instrument may only be operated within the scope of its intended use as described above
and as per the instructions given in this manual.
Any other use of this instrument is considered as improper operation.
2-2 Instrument Features

The HM525 NX is a precision-engineered, ergonomically-designed, high-performance
cryostat in modular design with a stainless steel cooling chamber. Some of its features are:
 Colour touch-screen user interface.

 A Lighted window for easy positioning.
 Intricate freezing device down to max. -55°C via the cryobar
 Automatic or manual defrost
 Stainless Steel cooling chamber
 Chamber temperature regulation variable from +5°C down to -35°C
 Additional knee room.


Cat.No. 391471 Issue 2.0 – 2013
3 Technical Specifications
3-1 Mechanical Specifications

Depth 760 mm (29.9 in)
Width 640 mm (25.19 in) w/o handwheel
Height Range 1150 mm ( 45.3 in)
Maximum Weight 143 kg (315.2 lb)
3-2 Electrical Specifications

Dedicated line recommended.
Power Requirements 100V, 50Hz, 7A, +/-10%, 110-120V, 60Hz,
7A, +/-10%
220-240V, 50/60Hz, 4A, +/-10%
BTU 1020BTU/hour
3-3 Cooling Specifications

Cryobar Cooling (Peltier Element) Max. - 55°C (-67°F)
Defrosting Options Programmed / Immediate
3-4 Environmental Specifications

For Indoor Use Only
+5°C to +35°C (+41°F to +95°F)
Temperature (Operating Limits)
(at a max. Relative humidity of 60%)
+15°C to +30°C (+59°F to +86°F)
Temperature (Recommended
Note: Performance may deteriorate when operated outside
Operation)
of this range.
Temperature (Transport and

-20°C to +50°C (-4°F to 122°F)
Storage)
Relative Humidity Max. 60% RH up to 35°C
Altitude Up to 2000 m (6,500 ft)
Pollution Degree 2
Over Voltage Category II
Floor Loading Requirements 230 kg/m² (507 lb/ft²)
Safety Class I
45 dB (A) - measured with 1m distance to the

Sound Emission
instrument.

Cat.No. 391471 Issue 2.0 – 2013
R404a, filling amount: 100...120V 175g
Refrigerant
220...240V 175g
3-5 Microtome and Sectioning Specifications

Section Thickness Range 1 – 500 µm
Fine Section Thickness Range 1 – 100 µm
1 – 10 µm in 1 µm steps
Fine Section Thickness Resolution 10 µm – 20 µm in 2 µm steps

20 µm – 50 µm in 5 µm steps
50 µm – 100 µm in 10 µm step
Trimming Thickness Range 5 – 500 µm
5 – 30 µm in 5 µm steps
Trimming Thickness Resolution
Specimen Retraction (Return Travel) 20 µm
Vertical Specimen Stroke 64 mm
Horizontal Specimen Movement 28 mm
Specimen Approach Automated via arrow buttons
30, 40 mm (additional special sizes are

Chuck Size
available)
x - and y - axes universal 8° with zero

Specimen Orientation
positioning; z - axis up to 360°
Coarse Feed Motorized
Cutting Drive Manual via handwheel
Handwheel Lock Mechanical
Clearance Angle Disposable blade carrier 8- 16°

Adjustment

Cat.No. 391471 Issue 2.0 – 2013
3-6 Default Settings
Counter Mode C=0
Retraction ON
Language English
Specimen head -20°C
Fine 0.5 µm
Trim 5 µm
Chamber Light 100%
Screen Brightness 100%
Stepper Motor Initialization Yes
Cryobar OFF
Disinfection, Cycle (Automatic) ON (03:00 Night)
Defrost Cycle (Automatic) ON (00:00 Night)
Section Counter 0
Section Sum 0

Cat.No. 391471 Issue 2.0 – 2013
4 Maintenance Methods
ELECTRICAL SAFETY, DISCONNECT THE CRYOSTAR FROM THE
MAINS SUPPLY BEFORE CARRYING OUT ANY OF THESE
PROCEDURES.
MECHANICAL SAFETY, THE CRYOSTAR CONTAINS MOVING

PARTS; TAKE ALL NECESSARY PRECAUTIONS BEFORE CARRYING
OUT ANY OF THESE PROCEDURES.
MECHANICAL SAFETY, REMOVE ALL BLADES OR KNIVES BEFORE

WORKING IN THE CHAMBER OR AROUND THE MICROTOME.
BIOHAZARD, ENSURE THAT THE CRYO CHAMBER HAS BEEN

DECONTAMINATED BEFORE CARRYING OUT ANY PROCEDURE
THAT REQUIRES WORKING INSIDE THE CRYO CHAMBER.
POTENTIALLY HAZARDOUS SUBSTANCES, THE CRYOSTAR

CONTAINS POTENTIALLY HAZARDOUS SUBSTANCES (E. G.
DISINFECTANT). TAKE ALL NECESSARY PRECAUTIONS WHEN
WORKING WITH THESE SUBSTANCES.
MICROTOME MAY BE COLD TO HANDLE AFTER POWER SHUT

OFF, HANDLE WITH PROTECTIVE GLOVES

Cat.No. 391471 Issue 2.0 – 2013
4-1 Dismantling the Casing
Note
All metal casing parts are secured with a grounding connection, to remove the casing parts you
have to disconnect this connection. Please ensure that you reconnect this connection
when you reassemble the casing parts again.
4‐1‐1 Remove the Rear Panel
Tools required:
 Phillips Screw Driver
Parts required:
 None
Procedure:
Use a Phillips Screw Driver to remove the 10 Phillips screws that secure the rear panel. Carefully lift the
rear panel off and place it to the side.

Cat.No. 391471 Issue 2.0 – 2013

4‐1‐2 Open the Top Cover
Tools required:
 Screw Driver
Parts required:
 None
Procedure:
Open the heated window.
Use a Screw Driver to remove the two screws from inside the front of the top cover. Lift off the top cover.
The cover has a fixation on the side, if you lift it off to the end it will stay in this position.
Service
Manual Cryostat HM525 NX Thermo Fisher Scientific
Page 17
Cat.No. 391471 Issue 2.0 – 2013

4‐1‐3 Remove the Handwheel
Tools required:
 6 mm Allen Key
 Screw Driver
Parts required:
 404410 Cover disk
Procedure:
THE HANDWHEEL IS HEAVY. TAKE CARE THAT THE HANDWHEEL IS

SECURED BY ACTIVATING THE MECHANICAL BRAKE WHILE
UNSCREWING IT.
Use a screw driver to carefully remove the cap from the middle of the handwheel.
Use the 6 mm Allen Key to remove the screw holding the handwheel.
Note
The handwheel handle should be in the DOWN position before removing.
Be careful of the spring and the bolt of the mechanical brake mechanism when you slide the handwheel
down.
To reassemble the handwheel again, position it in the high position and the specimen head also. Now you
can see the bolt through the hole in the handwheel. Push them together and fix the handwheel with the
Allen screw.

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4‐1‐4 Remove the middle part
Tools required:
 3 mm Allen Key
Parts required:
 None
Procedure:
Open the top cover – see 4-1-2 Open the top cover.
Use the 3 mm Allen Key to remove the two screws that secure the middle part. Carefully lift the part off
and place it to the side.
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4‐1‐5 Remove the front panel
Tools required:
 3 mm Allen Key
Parts required:
 None
Procedure:
Open the top cover – see 4-1-2 open the top cover.
Use the 3 mm Allen Key to remove the three screws at the front and lift off the front panel and place it to
the side.

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4‐1‐6 Remove the Foot panel
Tools required:
 3 mm Allen Key
 8 mm Wrench
Parts required:
 None
Procedure:
Remove the rear panel – see 4-1-1 remove the rear panel. Open the top cover – see 4-1-2 open the top
cover. Remove the front panel – see 4-1-5 remove the front panel.
Use the 3 mm Allen Key to remove the two screws at the right side and the one on the bottom. Use the 8
mm wrench to undo the nuts on the other side of the foot panel.

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Cat.No. 391471 Issue 2.0 – 2013

4‐1‐7 Replacement of Electronic Components

Following replacement of any electronic component (see list below), carry out the following steps to
check the functionality of Cryostat.
‐ Turn OFF the Cryostat
‐ Check the wiring connections between the new component and the C‐ECU, according to the
wiring diagram
‐ Insert the Thermo USB‐stick.
‐ Turn ON the Cryostat; the Login‐Screen appears.
‐ Press LOGIN and enter the Service password.
YOU MUST CARRY OUT THE FOLLOWING STEPS TO TEST ALL

ELEMENTS.
‐ Press the „SOFTWARE UPDATE“‐button to check the on‐board software versions. If required
update the appropriate software to the most‐ current version.
‐ Press the “INPUTS”‐button. The Service‐Inputs screen appears. Check the indicated switches.
The switches light barrier top reversal point and Microtome home position are normally on,
as indicated by a yellow light. The remaining switches are normally off and no yellow light
shows. The test is successful when the indicator changes color.
‐ Press the „OUTPUT“‐button. The service‐Output Screen appears.
‐ Press the “ON”‐button for chamber light. Press the “INPUTS”‐button and review the color
status of the relevant components. Green = OK, Red = current very high, yellow = not
connected or current very low, White = not ON or not connected.
‐ For other components press the relevant button (e.g. Compressor) and follow the above
procedure.
‐ To resolve/repair any red‐ or yellow – colored value follow the procedure in the relevant
troubleshooting tree.
‐ Create a service protocol to log all repairs completed.
‐ Perform the fully automated test but abort the test on upon reaching the COOLING A step.
‐ Now perform the Semi automated test to ensure full system functionality.
‐ Check the Service log‐file to ensure that all error messages have been cleared.
‐ When all “INPUTS“ and “OUTPUTS“ are checked press the “RETURN”‐button to display the
main screen. The chamber light is now illuminated and the Specimen head advances to the
home position.
‐ Press the “DATA LOG”‐button on the menu‐settings screen. Ensure that all error messages
have been cleared.
‐ Remove the USB‐Stick and restart the Cryostat.
Service
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Cat.No. 391471 Issue 2.0 – 2013

Note
In case of replacement of any electronic component, always perform the above procedure.
List of Electronic Components
‐ Replace defrost heater, defrost sensor, Cryobar Peltier
‐ Replace Compressor, Fan, chamber temp sensor and ambient temp. sensor
‐ Replace C‐ECU box and Cables
‐ Replace Power Supply (24V)
‐ Replace heating window , chamber light and rear contact switch
‐ Replace Electronic Connector panel (line filter, main switch, USB etc.)
‐ Replace Touch screen/TFT
‐ Replace fuses
‐ Replace C‐ECU Fan

Service
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Cat.No. 391471 Issue 2.0 – 2013

4-2 Condensing Unit
4‐2‐1 Replace the complete Condensing Unit
Tools required:
 5 mm Allen Key
 22 mm Wrench
 24 mm Wrench
 10 mm Wrench
 Evacuation Equipment
Parts required:
 435380 (115V) / 435370 (230V) Condensing Unit assembly
 261690 Armaflex Insulation tape
Procedure:
Power down unit and unplug it.

Remove the rear panel of the instrument – see 4-1-1 Dismantle the Rear Panel.
Use the Allen Key to unscrew the two screws on the bottom left hand side and remove the nut in the
middle with the 10 mm wrench. Lift up the whole condensing unit a little bit and move it to the left side
approximately 10 cm (4 inches).

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Cat.No. 391471 Issue 2.0 – 2013

Remove the Armaflex insulation from the tubing around the two quick couplings.
Use two wrenches size 22/24 mm to disconnect the two quick couplings.
Disconnect the wiring from the condensing unit and remove it.
Insert the new condensing unit assembly into the instrument in reverse order.
THIS AND ALL OTHER WORK MUST ONLY BE CARRIED OUT BY
AUTHORIZED REFRIGERATION TECHNICIANS WHO HAVE THE
CORRESPONDING TOOLS AND DEVICES AT THEIR DISPOSAL!
Use a refrigeration evacuation and filling system (i.e. ATP Promax Minimax®) to remove all old
refrigerants from the system for recycling.
Note
Dispose of the refrigerant in accordance with your national rules and regulations.

Cat.No. 391471 Issue 2.0 – 2013

Evacuate the system and refill it with refrigerant (R404A – 175g +/- 2g).
Note
The evacuation process takes a minimum of 45 minutes.
Turn on the instrument and plug in the service USB-Stick.

Enter the Service Menu and execute the condensing unit test procedure – see chapter 5, Test Procedures.
Service
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Cat.No. 391471 Issue 2.0 – 2013

4‐2‐2 Replace the Cooling Fan

Tools required:
 5mm Allen Key
 Ratchet
 10 mm socket
 250 mm Extension
Parts required:
 525080 (115V) / 525090 (230V) Cooling Fan assembly
Procedure:
Disconnect the fan from the connector box at the compressor.
Unscrew the two nuts (1) from the fan bracket mounted to the frame and remove the fan assembly.
Insert the new fan assembly and tighten the two nuts.
Reconnect the fan with the connector box.
Fix the condensing unit assembly with the two screws to the frame.
Reassemble the rear panel of the instrument.

Cat.No. 391471 Issue 2.0 – 2013

4‐2‐3 Replace the Starting Relay

Tools required:
 5mm Allen Key
 Phillips Screwdriver
Parts required:
 362810 (115V)/ 362820 (230V) Starting Relay
Procedure:
Remove the cover from the connector box, which is attached to the compressor, to access the starting
relay.
Remove the wiring from the relay.

Remove the faulty relay and insert the new one
Reconnect the relay.
Reinstall the cover of the connector box.
Reassemble the rear panel of the instrument.


Cat.No. 391471 Issue 2.0 – 2013

4‐2‐3‐1 Wiring of the Compressor
Service
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4-3 Heated Window

Tools required:
 3 mm Allen Key
 Wire Cutter
Parts required:
 625940 Heated Window assembly
Procedure:
Open the top cover – see 4-1-2 Open the Top Cover.
Disconnect the heated window from the socket and cut the wire tie.
Remove the heated window and replace it with a new one if required.
Note
Ensure that the window moves freely and that there is no excessive side to side movement. Use
the long guide rail to set a gap of 1 mm, side to side, between the heated window and the rail.
Reconnect the heated window and close the top cover.
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4-4 Evaporator Heater Mat & Sensor
THERE MAY BE SHARP EDGES IN THIS AREA; CARE SHOULD BE

TAKEN WHEN WORKING NEAR THE EVAPORATOR.
Tools required:
 2.5 mm Allen Key
 3 mm Allen Key
 Long Nose Pliers (or similar)
Parts required:
 626470 Heater Mat
 320900 Evaporator Temp. Sensor
 2 Cable ties
Procedure:
Remove the rear panel – see 4-1-1 Dismantle the Rear Panel.
Open the top cover – see 4-1-2 Open the Top Cover.
Remove the heated window.
Remove all screws from the evaporator cover.
Remove the socket for the heated window connector.
Remove the white screen from the front of the evaporator.
Heater Mat
Disconnect the heater mat from the C-ECU at the connector X15-A, 1+2.
Use long nose pliers (or similar) to ease the heater mat forward from underneath the evaporator and out of
the instrument.
Push the new heater mat under the evaporator.
Reconnect the heater mat.
Note
The heater mat is not fixed in position.
After replace ensure bottom of the heater mat is uniformly touching along the bottom of the
metal tray, this will ensure equal heating

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Evaporator Temp Sensor
Disconnect the electrical connection to the temp sensor at X15-A, 3+4.
1 Heater Mat 626470 2 Evaporator Temp. Sensor 320900
Ease the insulation upwards to gain access to the temp sensor.
BE CAREFUL NOT TO DAMAGE THE INSULATION.
Cut the cable ties that are holding the temp sensor in position and remove it.
Use new cable ties to hold the new temp sensor in position.
Connect the sensor.
Replace the white screen in front of the evaporator.
Replace the insulation.
Refit the evaporator cover, the heated window and the top cover.
Note
Check the movement of the heated window and adjust it if required.

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4-5 Electronic Control Unit (C-ECU)

Tools required:
 5 mm Allen Key
Parts required:
 435590 Electronic Control Unit assembly
Note
The C-ECU is protected by a metal cover and is replaced as a complete assembly.
Procedure:
Remove the rear panel– see 4-1-1 Dismantle the Rear Panel.
Disconnect all plugs from the C-ECU assembly.
Note
Note the positions for reconnection.
Use the 5 mm Allen Key to remove the four M6 Allen screws that secure the C-ECU and remove it.
Replace the C-ECU assembly as required and secure it to the chassis using the four M6 Allen screws (as
above).
Reconnect all of the plugs.
Replace the rear panel.

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4-6 C-Touch Panel

Tools required:
 2.5 mm Allan Key
Parts required:
 436210 Touch Screen
Procedure:
Remove the two screws from the rear of the touch panel housing and move the touch panel forward.
Disconnect the electrical connections to the touch panel and remove it from the top cover assembly.
Remove the SD-Card from the old Touch Screen
Insert it into the new Touch Screen
Replace the touch panel as required.
Reconnect the electrical connections.
Fit the touch panel back into the housing ensuring that the outer gasket is retained in position.
Refit the two screws that secure the touch panel screen to the housing.
Restore Data:
The SD-Card is a backup of the data from the Touch Screen. Information like operating hours, serial
number and quality test are saved on this card. To keep this data in case of a defective Touch Screen,
insert the card from the old Touch Screen in the new one.
After restarting the instrument with the new Touch Screen the window below will appear:
Please push this button to copy the files from the SD-Card to the new Touch Screen.
Note
If you don’t perform this copy procedure the Touch Screen will overwrite the SD-Card and all
old information on the card will be deleted.

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4-7 Cryobar Peltier, Blade Holder and Chamber Temp

Sensor
BIO-HAZARD, MAKE SURE THAT THE CRYO CHAMBER HAS BEEN
DECONTAMINATED BEFORE WORKING INSIDE THE CHAMBER.
REMOVE ALL KNIVES / BLADES BEFORE WORKING IN THE

CHAMBER.
Cryobar Peltier
Tools required:
 2 mm Allen Key
Parts required:
 336140 Peltier Element
Procedure:
Use the Allen Key to remove the fast freezing station from the cryobar.
Disconnect the electrical connection to the cryobar at the plug.
Remove the peltier from the cryobar assembly.
Clean out any residual adhesive and thermal compound.

Insert the cable from the new peltier through the access hole in the cryobar assembly.
Attach the new peltier to the cryobar assembly using the adhesive thermal compound.
Replace the fast freezing station.
Note
Don’t over tighten the screw because it might crack or damage the peltier
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4-8 Microtome
BIO-HAZARD, MAKE SURE THAT THE CRYO CHAMBER HAS BEEN
DECONTAMINATED BEFORE WORKING INSIDE THE CHAMBER.
REMOVE ALL KNIVES / BLADES BEFORE WORKING IN THE

CHAMBER.
ENSURE MICROTOME IS DRY AND SAFE TO HANDLE DUE TO COLD
TEMPERTURE
Note
In order to remove the Microtome it is first necessary to remove the Waste Tray, Right Chamber
Cover, Left Chamber Cover and the Blade Holder. Disconnect the electrical connector on the left
side of the microtome and remove the temperature sensor.
Tools required
 5 mm Allen Key
Parts required
 None
Procedure
Open the top cover see 4-1-2, remove the heated window see 4-3, use the 5 mm Allen Key to
remove the M5 screw that secure the complete Microtome in the chamber.
Note
Turn the hand wheel into the lower position, i.e. the Specimen Head is also at the lower reversal
point.
Slightly move the Microtome to the left approximately 2cm until the clutch on the right side of the
Microtome is free.
Remove it carefully from the chamber.
To reinstall the Microtome follow the steps in reverse order.
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4-9 Installation and Removal of the Cross Roller Bearings

Tools required
 2 mm Allen Key
 2.5 mm Allen Key
 3 mm Allen Key
 Torque Key
Parts required
 518330 Cross Roller Bearings
Procedure
Removing:
Remove the Microtome from the chamber see 4-8 and place it on a bench. Loosen the Allen screws which
fix the cover on the Microtome and lift it off.
To remove the specimen head undo the 5 mm Allen Key screw in the centre of the head. To get
access to the cross roller bearings remove the 4 Allen key screws that fixes the plastic plate
Remove the right roller bar.

Remove the two cross roller bars from the mono block.
Remove the cross roller bar from the left.

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Installing:
E D C B
A
1
Assemble all cross roller bars as disassembled, don’t tighten the screws. Place the screws slightly in place so
that the screw heads are touching the bottom of the cross roller bar and the cross roller bars touch the
base.
Make sure the cross roller bearings are pushed all the way to the right. Push the row (B) against the wall
and tighten it. Use the following sequence of fastening:
○ 1
○ 2
○ 2
○ 3
○ 3
Set a torque of 9Ncm with the torque key on the adjustment screws (A) on the left side. Fasten the screws
beginning at the right bar (C).
Repeat this step for all cross roller bars from C to E.
Reassemble the specimen head and the blade holder.
Use the fastening sequence for all rows.
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4-10 Installation and Removal of the Spindle, Cylinder and

Motor
Tools required
 362200 Adjusting Face Spanner
 362110 Key for Micrometer Spindle
 362090 Key for Micrometer Nut
 2 mm Allen Key
 3 mm Allen Key
 Pliers
Parts required
 550220 Spindle, Nut and Bearings
Procedure
Remove the microtome – see 4-8 Microtome. Remove the specimen head- see 4-9. First loosen the Multi
Function Cable. Next, dismount the Microtome. Loosen the Flexible Coupling from the end of the
Micrometer Assy. by unscrewing the 2mm screw through one of the access holes on the Distance Sleeve.
Now you can remove the 4 3mm bolts that fix the Stepper Motor to the Distance Sleeve.
To remove the Micrometer mechanism from the Cylinder proceed as follows:

Use the Adjusting Face Spanner (cat. no. 362200) to hold the Threaded Ring in place and at the same
time use the Key for the Micrometer Spindle (cat. no. 362110) to turn the Spindle from the front in a
counter clockwise direction. Now you are able to remove the Equalizing Discs, the axial bearing discs, the
axial needle wheel and the axial disc from the micrometer spindle. Next use the Offset Key for the
Micrometer Nut (cat. no. 362090) to remove the Spindle and Nut Complete. To further disassemble the
micrometer nut and the Counter nut, unscrew them from micrometer spindle.
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4-11 Installation and Removal of the Link Block

Tools required
 Allen Key 2.5 mm
 Allen Key 4 mm
Parts required
 520110 Link Block Service Set
Procedure
Remove the Microtome – see 4-8 – take off the metal cover that you have access to the shaft
with bearing. Remove the 4 Allen key screws and carefully pull out the shaft with bearings.
Note
Before installing a new link block, clean all bearing surfaces.
Remove old grease and lubricate the bearings surfaces as well as the cylinder pin with isoFlex
grease (cat.no 350140).
Check the link block for smooth movement on the guide rail. If the movement on the guide
rail is not smooth, carefully sand the link block with sandpaper (1000 grade).
Note
Be careful! Do not over-sand.
Besides the nominal size of 15.00 mm, link blocks with diverging tolerances of 15.02 mm or 14.98 mm
are available for special needs:
Standard size: 15.00 mm, cat. no. 421050

Oversize: 15.02 mm, cat. no. 421060
Undersize: 14.98 mm, cat. no. 420630
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4-12 Microtome home position switch adjustment

Tools required
 3 mm Allen Key
 Screw driver
 614150 Checking line for Microtome
Parts required
 none
Procedure
Move the Specimen head to its end position by pushing the button on the display (furthest position away
from the blade holder).
Switch off the instrument.
Remove the Microtome see 4-8, remove the specimen head see 4-9 that you have access to the spindle.
Use the micrometer spindle tool and rotate counter clockwise until you reach the mechanical end position.
From this point rotate the spindle in the opposite direction by 3-5 revolutions. Check if the home position
light barrier is still engaged. Flag (2) shouldn’t be engaging light barrier board if so then adjust the flag
with the screws (3).
Note:
Ensure that the Flag is absolutely parallel to the light barrier to have a clear switching point
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4-13 UV-Light (optional)

Tools required
 Screw driver
Parts required
 346230 UV-lamp
Procedure
Open the Top cover see, 4-1-2 open the top cover. Turn the heated window over so that you have access
to the UV lamp. Disconnect the UV lamp and put the new one in.
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5 Analysis and Troubleshooting

This chapter supports service technicians in self-contained searches for malfunctions, their corresponding
analysis and elimination.
Because of the technical and mechanical complexity of the CryoStat HM525 NX, this chapter is divided
into the different modules. This structure provides the reader with a more efficient overview.
The described failures and malfunctions might occur during the daily use of the CryoStaT HM525 NX.
Note!
If this information is not sufficient to fix a malfunction or failure, please contact the Factory
Service Support Team immediately.
5-1 Enter the Service Menu

The Service Menu provides the software tools and test procedures for the service technician.
To enter the Service Menu:
Press the “Menu” button on the Main screen.
The Menu screen appears.
Press the “Service” button.

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The Password screen appears.
Note
The password to enter the Service menu is walldorf. All in small letters.
Press the “Selection” button once to highlight the small letter “a”.
The keyboard will show small letters.
Use the keyboard to type in the password.
Press the “OK” button to enter the service menu.
Press the “Back” button to return to the Main screen.

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5-2 Overview of the Service Menu

The buttons on this screen show the available Service options.
Press the “Back” button to return to the Main screen
5‐2‐1 Selftest Final Check

Enter the Service menu as described above.
Press the “Selftest Final Check” button.
The “Service – Selftest Final Check” screen will appear.

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To perform the Fully Automated Test (FAT):
Note
The Fully Automated Tests checks all electromechanical functions of the CryoStaT. An on-screen
status report is provided at the conclusion of the Fully Automatic Test.
Press the “Fully Automated Test” button.
The “Service - Selftest Final Check - Fully Automated Test” screen appears.
Press the “Start” button to start the Fully Automated Test.
The “Service - Selftest Final Check - Fully Automated test” screen appears.
Note
The ‘‘Back’’ button is deactivated during this test routine.
Press the “ABORT” button to stop the test routine.

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Press the “Back” button to return to the Service – Self Test Final Check screen.
Press the “Back button to return to the Service menu.
Notes
The elapsed time since the „START‘‘-button was activated is shown on the top left of the screen.
Errors are listed in the uppermost grey field. The middle grey field is the status bar. The length of
the yellow bar reflects the progress of the test. The lowest grey field indicates the test routine
currently in progress.
An on-screen status report is provided at the conclusion of the ‘‘Fully-Automated Test’’.

The Fully Automated Test checks all of the following components:
Components
1. Chamber lighting LED (front)

2. Fan (C-ECU)
3. Window Heater
4. Defrost Heater
5. Peltier element (cryobar)
6. Home Switch Microtome & Stepper Motor
7. Compressor temperature sensor
8. Ambient temperature sensor
9. Defrost temperature sensor
10. General cooling test (approx 9 hours)
The Routine during fully Automated Test:

Cooling A 3 min to 6 hours -35°C/6h Chamber Temperature
Cooling B 1 hour -25°C Chamber Temperature
Cooling C 1 hour -20°C Chamber Temperature
Defrost Max. 60 minutes
Test Finished
Reboot and Start Normal GUI

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Press the “Semi Automated Test” button.
The “Service - Selftest Final Check - Semi Automated Test” screen appears.
Note
The upper gray field is the status bar. The length of the yellow bar reflects the progress of the
current test. The lower gray field identifies the test in progress. On completion of the test, the next
test is identified on the lower grey field. Press SKIP/ FAIL/ OK to proceed.
Press the “OK” button to start the Semi Automated Test.
Press the “SKIP” button to skip the current test and to move on to the next
Press the “FAIL” button to record a failed test in the error log and to display the next available test.
Press the “Back” button to return to the previous menu.

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To perform the Semi Automated Test (SAT):

The “Semi Automated Test” provides the capability to test selectable, individual system functions. Neither
temperature tests nor checking of temperature sensors is included in the “Semi Automated Test”. In the
“Semi Automated Test” the following test options are presented sequentially. If the test passes press the
“OK” button to display the next available test. If the test fails, press the “FAIL” button to record the
failure in the error log and to display the next available test.
1. Press Trim key to test 10µm ?

2. Press Trim key to test 500µm ?
3. Open Heated Window
4. Close Heated Window
5. Does the Cryobar Temp Reach its Max Temp within 2 Minutes ?
6. Cryobar Cooling Test Timed Out
7. Semi-Automated Test Completed
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5‐2‐2 Maintenance
This menu provides access to the following tasks:
 View and export error logs.

 View and export maintenance logs with time stamps and PM record.
 Service maintenance protocol.
 Log-File listing all service events.
To enter the “Service – Maintenance” screen:
Press the “Maintenance” button on the Service screen to access the “Service – Maintenance” screen.
The “Service - Maintenance” screen appears.
Logfile:
To export the logfiles:
Press the “Logfile” button.

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The “Service - Maintenance - Logfile” screen appears.
Press the “Export” button to save the log files to a USB-Stick.
Note
The log files can be subsequently exported to an Excel file.
Press the “Back” button to return to the “Service - Maintenance” screen.
The following events are automatically saved in the logfile:
 Complete listing of error-messages.

 Error log information will be saved for a period of one year following FIFO (“first-in, first-out”)
and then archived to a SD-memory card.
 Log File information is maintained in a temporary file which, when full, automatically archives
the information to the SD-memory card.
 The log file information also provides the serial number of the cryostat as well as system
configuration information in logfile header.
 The following information is saved on the SD-memory card: user logs, error logs, PM-protocols,
operating settings, instrument serial number and build configuration.
Note
To export the user log file in the user Application you must enter the Password
‘‘dataLog’’ with a big ‘‘L’’. When you export this file it will include the Quality Test
results, the Maintenance Protocols, the Service and user Log files.

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Maintenance Protocols:
This menu provides access to the following tasks and information:
 Information on Service tasks and completion dates.

 Creating of a maintenance-worklist.
 Editing according to PM-protocol.
 Checkbox on every single test, must be timestamp.
 Space for inclusion of the Field Service Engineer’s name (must be filled in) and for notes
(optional).
To access the “Service - Maintenance - Maintenance Protocols” screen:
Press the “Maintenance Protocols” button.
The “Service - Maintenance - Maintenance Protocols” appears.
Press on an existing maintenance protocol to review or export it.
To create a new maintenance protocol:
Press the “New Protocol” button.

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The “Service - Maintenance - Maintenance Protocols - (date)” screen appears.
Notes
Unchecked tasks are marked with a question mark.
Checked tasks are marked with ‘‘Done’’.
Press the “Question mark” button to mark the completed task as “Done”.
Note
It is mandatory to fill in the name of the Service technician performing maintenance on the
instrument.
Use the scroll bar on the left side to scroll down to the “Name” field.
Press on the “Name” button to enter your name using the appearing keyboard.
Press the “OK” button to save your name and to return to the “Service - Maintenance - Maintenance
Protocols - (date)” screen.
Press the “Export” button to export the maintenance protocol to the USB-Stick.

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Check that the following information is saved to the SD-memory card: user logs, error logs, PM-protocols,
operating settings, instrument serial number and build configuration.
Overview of the available Service Maintenance Items:
1. Condenser
2. Condenser Fan
3. Link Block
4. Knife Carrier Clamps
5. Microtome Guide Bars
6. Microtome Spindle
7. Microtome Advance Movement
8. Object Chuck Clamps
9. Cooling Performance
10. Defrost Function
11. C-Touch
12. Handwheel Zero Position
13. Handwheel Belt
14. Handwheel Brake Function
15. Crossroller Guide Bars
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5‐2‐3 Inputs
The “Service - Inputs” screen provides a status report on all system inputs, using the following color
highlighting:
White: Not connected

Green: O.K.
Red: Over Limit
Yellow: Below threshold value
To access the “Service - Inputs” screen:
Press the “Inputs” button on the Service screen to access the “Service - Inputs” screen.
The “Service - Inputs” screen appears.
Note
This screen displays inputs as ON/OFF, current (mA) and temperature (°C)

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5‐2‐4 Outputs
This menu is used to test the outputs of the CryoStar.
 Test the individual components of the cryostat.

 Use the “Inputs” and “Outputs” buttons to toggle between the two screens.
 Pressing the “Back” button cancels any changes made in the output screen.
To access the “Service - Outputs” screen:
Press the “Outputs” button on the Service screen to access the “Service - Outputs” screen.
The “Service - Outputs” screen appears.
This menu is used to:
 Check all outputs that can be directly tested.

 Check if input value change by pressing the appropriate ON-button.
 Check if all values are “OK” by toggling between the “Service - Input” and “Service - Output”
screens.
 Check if the switching between “Inputs” and “outputs” is working.
 Use the scroll-bar on the left to scroll up and down.
Press the “Back” button to return to the Service menu.

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5‐2‐5 Operating Hours
This menu provides access to Operating Hours functions. It provides the following tasks and information:
 Tabulates elapsed hours of operating for all electrical subsystems.

 Export of the operating hours logfile to a USB-Stick.
 The operating hours for all listed subsystems can be reset.
Notes
The operating hours log is saved for the life time of the instrument.
The operating power meter is automatically saved and provided with the maintenance protocol ---
see 5-2-2 Maintenance
To enter the “Service - Operating Hours” screen:
Press the “Operating Hours” button on the Service screen to access the “Service - Operating Hours”
screen.
The “Service - Operating Hours” screen appears.
Use the scroll bar on the right side to scroll through the screen.
Press the “Reset” button to reset the selected counter.

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A warning message appears.
Press the “OK” button to reset the selected counter.

Press the “Abort” button to return to the “Service - Operating Hours” screen without resetting the
counter.
Overview Operating Hours of electrical devices:
Reset not possible Reset possible
1. Unit X
2. Compressor X
3. Compressor Fan X
4. Stepper Motor X
5. Chamber LED Light X
6. UV-Lamp X
7. Power Supply X
8. C-Touch X
9. Display (Back LEDs operating hours) X
10. C-ECU X

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5‐2‐6 Software Update

This menu enables the Service Technician to perform Software updates on the instrument.
The following tasks can be performed:
 Software updates for

C-ECU
C-TOUCH
Language file updates
User Guide & Service Manual updates
To enter the “Service - Software Update” screen:
Press the “Software Update” button on the Service screen to access the “Service - Software Update” screen.
The “Service - Software Update” screen appears.
Note
The first field on this screen provides important information about the current installed software
version.
Update performed by a Service Technician:
The user GUI file can be updated directly by placing it in the /Service/Update/ path. All other files (user
GUI, Language, Service GUI and Service Manual files and folder) must be packed in a zip folder as
“ServicePack.zip” and placed on the USB-stick under /Service/Update/, or locally under /C-
Touch/Service/Update/.
Note
The files in ‘‘ServicePack.zip’’ will overwrite the old files.


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5‐2‐7 Update C‐Touch Software

The user GUI file can be updated directly by placing in the /Service/Update/ path.
Insert the USB-Stick in the USB-port on the connections panel.
The “Service - Software Update - C-Touch” screen appears.
Press the “check USB-stick” button.
When the USB-stick is recognised the name of the update file appear on the screen.
Press the file to highlight it.
Press the “Copy?” button.
Press the “Start C-Touch Update” button.


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Note
Within a short period the name of the new software version appears on the top left of the screen.
Reboot the C-Touch.

All other files (user GUI, Language, Service GUI and Service Manual files and folders) must be packed in
a zip folder as “ServicePack.zip” and placed on the USB-stick under /Service/Update/.
Insert the USB-Stick in the USB-port on the connections panel.
The “Service - Software Update - C-Touch” screen appears.
When the USB-stick is recognised the name of the update file appears on the screen.
Press the “Copy?” button.
Press the “Start additional Update” button.
Note
Within a short period the name of the new software version appears on the top left of the screen.
Reboot the C-Touch.
Press the “check local” button if the files have been saved locally on C-Touch and then update the files.

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5‐2‐8 Update C‐ECU Software

The user C-ECU firmware can be updated directly by placing in the /Service/Update/ path.
Insert the USB-Stick in the USB-port on the connections panel and reboot the instrument.
Lock in as Service.
Enter the Service menu and press the “Software Update” button on the Service screen to access the
“Service - Software Update” screen.
The “Service - Software Update - C-ECU” screen appears.
When the USB-stick is recognised the name of the update file appears on the screen.

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Press the “Load?” button.
Press the “Start Firmware Update” button.
Notes
The system now goes into the Bootloader Mode. It will take about 2 minutes to update the
software.
When the update is complete, the identity of the new software version and ‘‘Success’’ appears at
the top left of the screen.
Restart the instrument.
5‐2‐9 Factory Settings

This menu provides information about:
 System configuration or build options.

 Serial numbers and software versions.
To enter the “Service - Factory Settings” menu:
Press the “Factory Settings” button on the Service screen.
The “Service - Factory Settings” screen appears.

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System Configuration:
Press the “System Configuration” button.
The “Service - Factory Settings - System Configuration” screen appears.
Note
This screen is used to review the system build options for the Cryostat HM 525NX.
This screen provides a status report on system configuration, using the following color highlighting:
Yellow: This build option is implemented and available for use.

White: This build option is available but not implemented.
Note
The selected model is highlighted in yellow.
Pressing on the white button UV-Disinfection enables this option in the system software.
Press the “Back” button to return to the previous screen.
Serial Numbers:
This submenu provides information about the serial numbers of the installed electronic boards.
Press the “Serial Number” button on the “Service - Factory Settings” screen.

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The “Service - Factory Settings - Serialnumber” screen appears.
Note
Installed electronic boards: C-Touch and C-ECU
Press the “Back” button to return to the “Service - Factory Settings” screen.
Language Selection:
From the Main Menu, press the Language Selection button. The following screen will be
shown:
Select the required language and then press the return arrow button to go back to the main
screen.

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5‐2‐11 Reset Errors

Press the “Reset Errors” button on the Service screen to check on the effectiveness of a repair procedure.
Notes
The repair procedure is effective if the error message disappears from the screen and does not
return.
If the specific error message returns, recheck the original repair or look for another root cause for
the problem.
To verify and troubleshoot all error codes refer to the ‘‘ErrCodes-V0xx_Thermo.xlsx and
Limits.xlsx

Cat.No. 391471 Issue 2.0 – 2013

5‐2‐12 Error Codes

ErrorNo Name
001-001 Defrost Heating mat error
001-002 Evaporator temperature error
001-003 Defrost cycle interrupted either by User or by power failure. Defrost incomplete
Defrost time should not overlap with disinfection cycle.
001-004
Disinfection will begin 2 hours after completion of defrost cycle
001-005 Cryobar Peltier error
001-006 CPU fan error
001-007 Chamber light error
001-008 Defrost sensor error
002-002 Ambient Temperature error
004-001 Stepper home position error
004-007 Window heating error
004-011 Chamber temperatur sensor error
005-001 C-Touch communication error

Defrost time should not overlap with disinfection cycle.
007-002
Disinfection will begin 2 hours after completion of defrost cycle
007-003 Disinfection cycle interrupted either by User or by power failure. Disinfection incomplete
007-004 Close window to start disinfection cycle
007-009 Close window to start Disinfection
007-012 Close Window & Start Disinfection cycle once again
007-014 Efficiency of the UV-lamp reduced due to operating hours
008-002 Change UV-lamp

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5‐2‐13 Troubleshooting Trees

Error code Troubleshooting tree number Page
001-001 TT 05 Evaporator Heating 73

001-002/001-008 TT 06 Evaporator Sensor 74
001-005 TT 09 Cryobar Peltier 76
001-006 TT 01 CPU Fan 70
001-007 TT 03 Chamber Light 72
008-002 TT 04 UV-Light 72
002-002 TT 07 Ambient Sensor 74
004-001 TT 08 Contact Switch Microtome 75
004-007 TT 02 Window Heating 71
TT Startup Temerature error 77

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6 Service Assemblies and Spare Parts
Module / Description Part Number
Module: Chassis
Top Panel 443100
Front Panel 419650
Middle Panel Cryo Chamber 419660
Lower Cover Cryo Chamber (foot panel) 419670
Rear Panel 419630
Door 419640
Waste Bottle 261980
Heated Window 625940
Heated Window with UV-light (230V) 625950
Heated Window with UV-light (115V) 625960
Hand Wheel Assy, with screw and Cover Disk 534200
Cover Disk 404410
Window OPEN/CLOSE microswitch 322010
UV-lamp 346230
Module: Refrigeration
Refrigeration Module, 115 V 435380
Refrigeration Module, 230 V 435370
Fan Assembly Refrigeration Module, 115 V 525080
Start Relays Assembly, 115 V 362810
Fan Assembly Refrigeration Module, 230 V 525090
Start Relays Assembly, 230 V 362820
Cryobar Peltier 336140
Evaporator Temp Sensor 320900
Ambient Temp Sensor 320900
Evaporator Heater Mat 626470
Module: Electronic
Touch Screen 436210
C-ECU, Main PCB Box 435590
Power Supply Assy 630440
Main Plug (115V) 329620
Main Plug (230V) 321100
Line Filter 265020
USB-Stick 612070
Fuse Kit for C-ECU 626310

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Module: Microtome/ Blade Holder

Stepper Motor 320910
Light Barrier Board 550430
Crossroller Bearings 518330
Spindle, Nut and Bearings 550220
Specimen Head cpl. 556070
Link Block Service Set 520110
Shaft with bearing 403250
Guidance with socket 550240
Knife Holder C cpl. 705460
Blade Holder EC cpl. 705630
Blade Holder EC Upper Part 566210
Glas Anti Roll Plate 449980
Chamber Temp Sensor 320900
Link Block Kit 520110
Cryostat Oil 350040
Iso Flex Grease 350140
Special Tools:
Torque Key 362060
Inserts for Torque Key 362290
Dial Gauge 362070
Dial Gauge Holder 648010
Spring scale for micrometer mechanism 362080
Torque measurement device for micrometer spindle 358170
Key for micrometer spindle 362110
Adjusting face spanner Size 2 362200
Key for micrometer nut 362090
Checking line for Microtome 2.5m 614150
Service
Page 79
Cat.No. 391471 Issue 2.0 – 2013

Appendix A – Cryostat HM525 NX Service Levels

Maintenance levels
Level I: User maintenance
Installation
Turning-on
Cleaning
Installing accessories
Level II: Service technician maintenance

Routine maintenance
Installing accessories
Diagnostics
Replacement of assemblies and parts
Executing service contracts
Level III: Factory maintenance

Repair of assemblies and parts
Repair of major damage
Functions listed in the maintenance Level I can be carried out by the user and are included in the User
Guide. Work listed in Level III is carried out at the factory. For this, it is necessary to return the cryostat
microtome in its original packing.
Note
Maintenance Levels II and III may only be performed by trained service technicians.
Unauthorized modification or repair by third party persons will void the warranty.
Field maintenance techniques

Service and maintenance work listed in Level II is carried out by a trained service technician.
A routine Preventive Maintenance should be performed once a year:
DEFECTIVE ASSEMBLIES AND PARTS SHOULD BE RETURNED TO THE FACTORY FOR

REPAIR OR REPLACEMENT .A DESCRIPTION OF THE MALFUNCTION AS WELL AS THE
SERIAL NUMBER OF THE INSTRUMENT IS REQUIRED.
Service
Page 80
Cat.No. 391471 Issue 2.0 – 2013

Appendix B – PM Procedure
Tools required:
 Syringe
 Allen Keys (different sizes)
Parts required:
 520110 Link Block Kit
 350040 Cryostat Oil
 350140 Iso Flex Grease
Procedure and Checklist:
Step Done
Check the movable parts for good surface quality, dimensional accuracy and wear and
□
tear.
Check the Cross Roller Bearings for noise as well as the various torques. □
Check the feed and retraction behavior of the Stepper Motor via a Dial Gauge. □
Check the Drive Belt Tension and adjust if necessary. Check the smoothness as well as
□
the impact of the Handwheel and adjust if necessary.
Check/Set the Real/Defrosting Clock □

Check Cooling Power (and Temperature Control) according to the Specifications. □
Clean Condenser □
Lubricate all movable parts, Cross Roller Bearings with Cryostat Oil the Link Block and
□
the Microtome Columns with ISO FLEX Grease.
Empty the Waste Container Bottle. □
Service
Page 81
Cat.No. 391471 Issue 2.0 – 2013

Appendix C – Instrument and Service Modules Schematics
Appendix C‐1 Instrument Overview
No. Description See

1 C-ECU Module Appendix C-2
2 Condensing Unit Module Appendix C-3
not shown Microtome Appendix C-4

Service
Page 82
Cat.No. 391471 Issue 2.0 – 2013

Appendix C‐2 C‐ECU
1 C-ECU 435590 Fuse kit 626310

Cat.No. 391471 Issue 2.0 – 2013

Appendix C‐3 Microtome and Motor
1 Microtome 4 Stepper Motor 320910

2 Specimen Head cpl. 556070 5 Shaft with bearing 403250
3 Guidance with socket 550240 6 Light barrier board 550430
Service
Page 84
Cat.No. 391471 Issue 2.0 – 2013

Appendix C‐4 Condensing Unit
3 5
7 2
1 Condensing Unit 435380 5 Quick couplings

assembly(115V)
Condensing Unit 435370 6 Staring Relay(115V) 362810
assembly(230)
2 Fan assembly(115V) 525080 Staring Relay(230V) 362820
Fan assembly(230) 525090 7 Compressor
3 Evacuation outlets
4 Condenser

Service
Page 85
Cat.No. 391471 Issue 2.0 – 2013

Appendix D – Wiring Diagram

Cat.No. 391471 Issue 2.0 – 2013

Appendix E – Schematic of the Refrigerating System

Cat.No. 391471 Issue 2.0 – 2013

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Cryostat HM525 NX

Service Manual Cryostat HM525 NX Page 1

Microm International, GmbH

Subsidiary of Thermo Fisher Scientific

Subsidiary of Thermo Fisher Scientific

4481 Campus drive

This instrument conforms to the essential requirements of:

IVD Directive 98/79/EC

Service Manual Cryostat HM525 NX Page 2

Service Manual Cryostat HM525 NX Page 3

Errors and omissions excepted. Subject to amendment.

English (printed version): 388159

Print Date: 19/08/2013

Service Manual Cryostat HM525 NX Page 4

Service Manual Cryostat HM525 NX Page 5

Service Manual Cryostat HM525 NX Page 6

This symbol is used on the equipment, or in a document, to indicate that

appears on the instrument, always refer to the operator guide.

This symbol is used on the equipment, or in a document, to indicate that

This symbol is used on the equipment, or in a document, to indicate that

laboratory procedures. Take suitable precautions.

A WARNING IS GIVEN IN THE DOCUMENT IF THERE IS A

Service Manual Cryostat HM525 NX Page 7

1-3 General Safety

 Only use reagents recommended in the operator guide.

Service Manual Cryostat HM525 NX Page 8

 Customers using non-specified chemicals in the instrument, do so at their own risk.

 All chemicals recommended by Thermo Fisher Scientific have auto-ignition temperatures

Service Manual Cryostat HM525 NX Page 9

Please note that your warranty may be invalidated if:

 This instrument is modified in any way.

Service Manual Cryostat HM525 NX Page 10

Introduction to the Cryostat HM525 NX

Any other use of this instrument is considered as improper operation.

2-2 Instrument Features

 Colour touch-screen user interface.

Service Manual Cryostat HM525 NX Page 11

3-1 Mechanical Specifications

3-2 Electrical Specifications

3-3 Cooling Specifications

3-4 Environmental Specifications

Temperature (Transport and

Relative Humidity Max. 60% RH up to 35°C

Altitude Up to 2000 m (6,500 ft)

Over Voltage Category II

Floor Loading Requirements 230 kg/m² (507 lb/ft²)

45 dB (A) - measured with 1m distance to the

Service Manual Cryostat HM525 NX Page 12

3-5 Microtome and Sectioning Specifications

Fine Section Thickness Range 1 – 100 µm

Fine Section Thickness Resolution 10 µm – 20 µm in 2 µm steps

Trimming Thickness Range 5 – 500 µm

200 µm – 500 µm in 50 µm steps

Specimen Retraction (Return Travel) 20 µm

Vertical Specimen Stroke 64 mm

Horizontal Specimen Movement 28 mm

Specimen Approach Automated via arrow buttons

30, 40 mm (additional special sizes are

x - and y - axes universal 8° with zero

Coarse Feed Motorized

Cutting Drive Manual via handwheel

Handwheel Lock Mechanical

Clearance Angle Disposable blade carrier 8- 16°