PRIST UNIVERSITY

M.Phil BIOTECHNOLOGY
Paper II ADVANCED BIOTECHNOLOGY (Sub. Code:193BTC12)
UNIT – I

INTRODUCTION TO GENETIC ENGINEERING

Salient features of cloning vectors, restriction enzymes and their mode of action,
recombinant DNA- types of cloning vectors, plasmids, cosmids, M-13 Phage. Microinjection, particle
bombardment, electroporation (Vector less mode), Construction of genomic DNA library and cDNA
library.

UNIT– II

PLANT BIOTECHNOLOGY
Vectors for gene transfer ( Ti , Ri plasmids, cointegrate, intermediate and helper plamids) Binary
vectors, viruses as vectors gene transfer techniques using Agrobacterium, selectable markers, reporter
genes and promoters , transgenic plants, crop improvement , resistance to herbicide insects, pests and
viruses, cytoplasmic male sterility, delayed fruit ripening, antibody vaccine and interferon production
in plants, synthetic seeds.

UNIT – III

BIOPROCESS
Bioreactor designs and types of fermentation and fermentors. Concepts & basic modes of
fermentation - Batch, fed batch and continuous fermentation. Conventional fermentation versus
biotransformation. Solid substrate, surface and submerged fermentation. Fermentation economics and
fermentation media. Fermenter design - mechanically agitated, pneumatic and hydrodynamic fermenters.
Large scale animal and plant cell cultivation and air sterilization. Upstream processing - media formulation,
sterilization, aeration and agitation. Measurement and control of bioprocess parameters, scale up and scale
down process

UNIT- IV
TECHNIQUES IN BIOTECHNOLOGY
PCR- key concept- applications- PCR variants- analysis of amplified product by gel electrophoresis- Nucleic
acid blotting techniques- sequencing – Sangers method, Maxam and Gilbert method- primer walking-
automated sequencing- DNA chips and microarray-DNA fingerprinting.

UNIT – V

BIOTECHNOLOGY – INTELLECTUAL PROPERTY

i) Intellectual property of rights, patents, trade secrets, copyrights, trade mark, choice of Intellectual
Property Protection (IPR), and Plant genetic Resources ( PGR), GATT ( Genetic Agreement of Tariff
and Trade), TRIP ( trade Related Intellectual property).
ii) Patents for higher plants, patenting transgenic organism and isolated genes, patenting genes and DNA
sequences, Plant Breeders Rights ( PBR) and farmers Right.
iii) Ethical issues in animal biotechnology. Management aspects of biotechnology and genetic
engineering.
Paper II ADVANCED BIOTECHNOLOGY (Sub. Code:153BTC12) Question Bank

1. Explain the construction of genomic DNA library.

2. Elaborately discuss the vectors used in cloning.
3. Describe vector less cloning methods in detail.
4. Describe agrobacterium mediated gene transfer.
5. Explain how biotechnology can be used to increase the herbicide resistance in plants.
6. Write notes on Antibody vaccine & Synthetic seeds
7. Given detailed account on Types of Bioreactor.
8. Elaborate on the Batch and Fed-batch culture
9. Explain in detailed about Upstream and Downstream Process.
10. Discuss the mapping of human genome.
11. Discuss the role of genetic engineering in vaccine and hormone production.
12. Elaborate on the gene sequencing methods.
13. Discuss the ethical issues related to biotechnology.
14. What is Intellectual Property Rights? Discuss it in detail.
15. Define patent? Discuss the steps involved in patenting.
 PRIST UNIVERSITY

M.PHIL - BIOTECHNOLOGY

PAPER- III - ENVIRONMENTAL BIOTECHNOLOGY (Sub. Code: 153BTE13)

UNIT – I
Environment : Basic concepts –Environmental Pollution : Types of pollution, Pollution
monitoring and measurement; Methodology of environmental management – The problem
solving approach and its limitations.

UNIT – II
Biofertilizers- Defination, Distinguished Features of Biofertilizers and
Organic Manures. Role of symbiotic and asymbiotic nitrogen fixing bacteria in the
enrichment of soil, Algal and fungal biofertilixers (VAM). Bioleaching- Ore
Leaching and Role of Microbes in Mines ( copper, and Uranium). Environmental
significance of Genetically modified microbes, plants and animals. Bio- assessment
of environmental quality.

UNIT – III
Sewage/Waste water treatment - collection, Primary treatment, Secondary treatment –
Aerobic process: Activated sludge, Oxidation ditches, Trickling filters, towers, rotating discs,
rotating drums, oxidation ponds. Anaerobic processes: Anaerobic digestion, Anaerobic
filters, Tertiary treatment. Waste water treatment for some industries - dairy, distillery,
tannery, sugar, antibiotic industries. Water recycling.

UNIT – IV
Solid waste treatment and disposal - : Sources and management - Composting, vermiculture
and methane production. Biodegradation and bioremediation - Xenobiotics Hydrocarbons, oil
pollution, surfactants, pesticides.

UNIT – V
Global environmental problems: Ozone depletion, Green house effect and acid rain, their
Bioremediation of contaminated soils and waste land.
.

Reference book:
 Biotechnology by U.Sathyanarayana 2008. Books and allied (P) Ltd.

 Fundamental of Environmental Studies by Bharrgava, D.S

 PRIST UNIVERSITY

M.Phil - BIOTECHNOLOGY

PAPER III - ENVIRONMENTAL BIOTECHNOLOGY (Sub. Code: 193BTE13)

Question Bank

1. Write elaborately on environmental pollution.

2. What is pollution? Discuss how it is measured and monitored.

3. Discuss elaborately on the methodology of environmental management. Add a note on its merits
and demerits.
4. Given detailed acoount on Biofertilizers.
5. Write an account on classification of Bioleaching
6. Give a general account on biomonitoring and biomineing
7. Describe the functioning of trickling filters in purification of industrial effluents.
8. Discuss the anaerobic process of waste water treatment.

9. Write notes on the waste water treatment of effluents from a) dairy b) tannery and c) sugar industries.
10. Explain the types and applications of bioremediation.
11. Discuss the biodegration and bioremediation of Xenobiotics and pesticides.
12. Give a general account of vermin-composting.
13. Write notes on a) acid rain, b) green house effect.
14. Write elaborately on ozone depletion and its consequences.
15. Discuss the methods of reclamation of waste lands.